Rroxscaffold_2G00081630

OST-HTH/LOTUS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000679
Physical Location & Seq
Reverse (-)
4676326 .. 4678328
2003 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00081630.1

Sequence Viewer

Length: 1344 bp
ATGACGGAAATGAAAGAAACCCCTCTGCTTTTCGCTGTGAAGAATGAGAGCAAGGACTGTGCTAAGCTTCTTTTGACCTGGGGAGCAAATAGTGAAGTCCTTAATCTACGGCATACTCGTGCGTGTATATATCTTTACTTTGTTCATGGTTCTCAAGTTGTTACCCCTTTGAATGCTGGAAATGTCAACTCAAGCACCAAGGCAGACGTTTGTAAGTACTTCAACTGTCATCAAGCATGTCTCAGAGGAGCAAAGTGCTTTAATGCACATTGTGAGGAGTCTCAGAAAGTGAAGGAGGGAGAAGACAGGAATCATTCTTCTGCTGCTAAACTACTCAAACGCAAAATCTTTGTTGGAGGACTTCCTCCTTCAGTGGAGTCTGATGCTTTGAGAAAGCTCTGTGAAGAAGAGTTTGGATCGGTGGAAGATGCTGTAGTTATTGTAACTCAAATAGAAAACAAGATACAATCTCGAGGCTTTGGTTTTGTCACATTTAGAGGGGAGAAATCTGCTTCAGAAGCCATGCTAGAGCGCTACATCACCTTGAGAGGCAAGAAAGTTGAAATAAAAAGTTTGATTCCAAAATTGGTTTTGGGAGCTGACCTTGAGAAAATGTCACCTCAACCACAAGATCAAGAGAAGAATTTCCAATACCAGCTTCCACTGCAGAAGCCGAACGAGAATATTGTGGAAGGTGATAACCCTGAACAAAGTTCTTGGGCTGATAGATTAGTCCTTAAACAACCAGCAGCTTGTTCCATTGAACCTCAAGCACATGTTACCAAGAGCTCTGAGGACCCAAGCACTCCTACATGGCTAAAGGTCTGTCAAAGCATTCAAATTATGCTCTCTTCTCTAAAAGCTGACTTCAGGGCTAAATTTGGGTTAGAACTGGATCACTCATCTGTGGGCTACTCTAAGCTCAGTGACTTCCTGAAGCGTGTCTCCGATTTATGTACCATCAAGATTCTCCCCATATGCAAACATGGAACTCCAACTCACATGATCCTCCGTCCAAAATTTTCTAGTCCTCAATGCCGCCTCCTTCCTACACTCAGAACAACTCACACTCTGTCTTTGTCTGCATTTATTGTGAATGGTGGTGATTCAAAGTGTCTTTTATCCCTCTTAAGTAGCAATGGTGGTGATTTAAAGGATCTTCAGAACCTTTCAACTGACAGCAGTAGTGATGAGAAGTGTCTCCAGGACCTTCCAACTGACAATGCTATTGATTCAAAGTGCCTTCAGGACCTTTCACTTGATTCTGGTGGTGATTCAGAGTGTATTGGAGACGAAATGTATGGCTCAAGTGTCACTAGCCATGGTCTGGGTATCCTGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 OST-HTH RRM_1

Protein Analysis

447

Amino Acids

49.23

Weight (kDa)

6.61

Isoelectric Point (pI)

49.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 116 - 185 1.4e-11 RNA recognition motif
OST-HTH PF12872 281 - 322 1.3e-07 OST-HTH/LOTUS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1327
AciI CCGC 1 cut(s) 1039
AclWI GGATC 4 cut(s) 424, 903, 1000, 1164
AcsI RAATTY 3 cut(s) 643, 878, 1019
AcuI CTGAAG 6 cut(s) 354, 498, 853, 956, 1145, 1229
AdeI CACNNNGTG 1 cut(s) 272
AfaI GTAC 2 cut(s) 218, 958
AfeI AGCGCT 1 cut(s) 533
AfiI CCNNNNNNNGG 1 cut(s) 1327
AflII CTTAAG 1 cut(s) 1129
AflIII ACRYGT 1 cut(s) 775
AgsI TTSAA 8 cut(s) 172, 223, 563, 764, 839, 1110, 1173, 1236
AjnI CCWGG 2 cut(s) 77, 1203
AjuI GAANNNNNNNTTGG 4 cut(s) 396, 428, 642, 674
AluBI AGCT 8 cut(s) 67, 397, 599, 658, 752, 789, 863, 922
AluI AGCT 8 cut(s) 67, 397, 599, 658, 752, 789, 863, 922
Alw21I GWGCWC 1 cut(s) 791
Alw26I GTCTC 5 cut(s) 245, 285, 949, 1205, 1284
AlwI GGATC 4 cut(s) 424, 903, 1000, 1164
Ama87I CYCGRG 1 cut(s) 471
Aor51HI AGCGCT 1 cut(s) 533
ApeKI GCWGC 2 cut(s) 323, 749
ApoI RAATTY 3 cut(s) 643, 878, 1019
Asp700I GAANNNNTTC 1 cut(s) 644
AspLEI GCGC 1 cut(s) 534
AspS9I GGNCC 3 cut(s) 796, 1207, 1249
AsuHPI GGTGA 6 cut(s) 532, 609, 707, 1115, 1157, 1283
AvaI CYCGRG 1 cut(s) 471
AvaII GGWCC 3 cut(s) 796, 1207, 1249
BanII GRGCYC 1 cut(s) 791
BauI CACGAG 1 cut(s) 117
BbsI GAAGAC 1 cut(s) 309
Bbv12I GWGCWC 1 cut(s) 791
BbvI GCAGC 2 cut(s) 310, 761
BccI CCATC 1 cut(s) 968
BceAI ACGGC 1 cut(s) 125
BciT130I CCWGG 2 cut(s) 79, 1205
BciVI GTATCC 1 cut(s) 1343
BcoDI GTCTC 5 cut(s) 245, 285, 949, 1205, 1284
BfaI CTAG 3 cut(s) 527, 1026, 1317
BfmI CTRYAG 2 cut(s) 432, 665
BfoI RGCGCY 1 cut(s) 535
BfrI CTTAAG 1 cut(s) 1129
BfuI GTATCC 1 cut(s) 1343
BisI GCNGC 3 cut(s) 324, 750, 1039
BlpI GCTNAGC 1 cut(s) 63
BlsI GCNGC 3 cut(s) 325, 751, 1040
BmcAI AGTACT 1 cut(s) 218
Bme1390I CCNGG 2 cut(s) 79, 1205
Bme18I GGWCC 3 cut(s) 796, 1207, 1249
BmeT110I CYCGRG 1 cut(s) 471
BmgT120I GGNCC 3 cut(s) 796, 1207, 1249
BmiI GGNNCC 1 cut(s) 798
BmrFI CCNGG 2 cut(s) 79, 1205
BmsI GCATC 2 cut(s) 373, 418
BpiI GAAGAC 1 cut(s) 309
BpmI CTGGAG 1 cut(s) 1187
Bpu1102I GCTNAGC 1 cut(s) 63
BpuEI CTTGAG 6 cut(s) 138, 175, 565, 626, 753, 1291
BsaJI CCNNGG 3 cut(s) 78, 198, 1321
Bsc4I CCNNNNNNNGG 1 cut(s) 1327
Bse1I ACTGG 1 cut(s) 897
Bse3DI GCAATG 1 cut(s) 1144
BseBI CCWGG 2 cut(s) 79, 1205
BseDI CCNNGG 3 cut(s) 78, 198, 1321
BseLI CCNNNNNNNGG 1 cut(s) 1327
BseMI GCAATG 1 cut(s) 1144
BseMII CTCAG 5 cut(s) 256, 296, 783, 937, 1069
BseNI ACTGG 1 cut(s) 897
BseRI GAGGAG 2 cut(s) 261, 290
BseXI GCAGC 2 cut(s) 310, 761
BsiHKAI GWGCWC 1 cut(s) 791
BsiHKCI CYCGRG 1 cut(s) 471
BslI CCNNNNNNNGG 1 cut(s) 1327
BsmAI GTCTC 5 cut(s) 245, 285, 949, 1205, 1284
BsmBI CGTCTC 1 cut(s) 1284
BsmI GAATGC 2 cut(s) 178, 834
BsoBI CYCGRG 1 cut(s) 471
Bsp1286I GDGCHC 1 cut(s) 791
Bsp143I GATC 5 cut(s) 416, 631, 895, 1005, 1156
Bsp1720I GCTNAGC 1 cut(s) 63
Bsp19I CCATGG 1 cut(s) 1321
BspACI CCGC 1 cut(s) 1039
BspCNI CTCAG 5 cut(s) 255, 295, 784, 936, 1068
BspLI GGNNCC 1 cut(s) 798
BspMAI CTGCAG 1 cut(s) 669
BspPI GGATC 4 cut(s) 424, 903, 1000, 1164
BspTI CTTAAG 1 cut(s) 1129
BsrDI GCAATG 1 cut(s) 1144
BsrI ACTGG 1 cut(s) 897
BssECI CCNNGG 3 cut(s) 78, 198, 1321
BssMI GATC 5 cut(s) 416, 631, 895, 1005, 1156
BssSI CACGAG 1 cut(s) 117
BssT1I CCWWGG 2 cut(s) 198, 1321
Bst2BI CACGAG 1 cut(s) 117
Bst2UI CCWGG 2 cut(s) 79, 1205
Bst4CI ACNGT 2 cut(s) 59, 227
Bst6I CTCTTC 2 cut(s) 402, 856
BstAFI CTTAAG 1 cut(s) 1129
BstDEI CTNAG 7 cut(s) 63, 242, 282, 792, 918, 923, 1055
BstDSI CCRYGG 1 cut(s) 1321
BstH2I RGCGCY 1 cut(s) 535
BstHHI GCGC 1 cut(s) 534
BstKTI GATC 5 cut(s) 419, 634, 898, 1008, 1159
BstMAI GTCTC 5 cut(s) 245, 285, 949, 1205, 1284
BstMBI GATC 5 cut(s) 416, 631, 895, 1005, 1156
BstMWI GCNNNNNNNGC 2 cut(s) 518, 664
BstNI CCWGG 2 cut(s) 79, 1205
BstNSI RCATGY 2 cut(s) 240, 779
BstSCI CCNGG 2 cut(s) 77, 1203
BstSFI CTRYAG 2 cut(s) 432, 665
BstV1I GCAGC 2 cut(s) 310, 761
BstV2I GAAGAC 1 cut(s) 309
BstX2I RGATCY 1 cut(s) 1156
BstYI RGATCY 1 cut(s) 1156
BsuI GTATCC 1 cut(s) 1343
BtgI CCRYGG 1 cut(s) 1321
BtsI GCAGTG 1 cut(s) 662
BtsIMutI CAGTG 3 cut(s) 378, 662, 931
CfoI GCGC 1 cut(s) 534
Cfr13I GGNCC 3 cut(s) 796, 1207, 1249
Csp6I GTAC 2 cut(s) 217, 957
CviAII CATG 8 cut(s) 146, 237, 523, 776, 813, 986, 1003, 1322
CviQI GTAC 2 cut(s) 217, 957
DdeI CTNAG 7 cut(s) 63, 242, 282, 792, 918, 923, 1055
DpnI GATC 5 cut(s) 418, 633, 897, 1007, 1158
DpnII GATC 5 cut(s) 416, 631, 895, 1005, 1156
DraI TTTAAA 1 cut(s) 1152
DraIII CACNNNGTG 1 cut(s) 272
Eam1104I CTCTTC 2 cut(s) 402, 856
EarI CTCTTC 2 cut(s) 402, 856
Ecl136II GAGCTC 1 cut(s) 789
Eco130I CCWWGG 2 cut(s) 198, 1321
Eco24I GRGCYC 1 cut(s) 791
Eco47I GGWCC 3 cut(s) 796, 1207, 1249
Eco47III AGCGCT 1 cut(s) 533
Eco53kI GAGCTC 1 cut(s) 789
Eco57I CTGAAG 6 cut(s) 354, 498, 853, 956, 1145, 1229
Eco88I CYCGRG 1 cut(s) 471
EcoICRI GAGCTC 1 cut(s) 789
EcoO109I RGGNCCY 3 cut(s) 796, 1207, 1249
EcoRII CCWGG 2 cut(s) 77, 1203
EcoT14I CCWWGG 2 cut(s) 198, 1321
EcoT38I GRGCYC 1 cut(s) 791
ErhI CCWWGG 2 cut(s) 198, 1321
Esp3I CGTCTC 1 cut(s) 1284
FaeI CATG 8 cut(s) 149, 240, 526, 779, 816, 989, 1006, 1325
FatI CATG 8 cut(s) 145, 236, 522, 775, 812, 985, 1002, 1321
FauNDI CATATG 1 cut(s) 977
Fnu4HI GCNGC 3 cut(s) 324, 750, 1039
FriOI GRGCYC 1 cut(s) 791
Fsp4HI GCNGC 3 cut(s) 324, 750, 1039
FspBI CTAG 3 cut(s) 527, 1026, 1317
GlaI GCGC 1 cut(s) 533
GluI GCNGC 3 cut(s) 324, 750, 1039
GsuI CTGGAG 1 cut(s) 1187
HaeII RGCGCY 1 cut(s) 535
HhaI GCGC 1 cut(s) 534
Hin1II CATG 8 cut(s) 149, 240, 526, 779, 816, 989, 1006, 1325
Hin6I GCGC 1 cut(s) 532
HinP1I GCGC 1 cut(s) 532
HincII GTYRAC 1 cut(s) 187
HindII GTYRAC 1 cut(s) 187
HindIII AAGCTT 1 cut(s) 65
HinfI GANTC 9 cut(s) 278, 310, 377, 577, 967, 1106, 1232, 1262, 1274
HphI GGTGA 6 cut(s) 532, 609, 707, 1115, 1157, 1283
Hpy166II GTNNAC 1 cut(s) 187
Hpy188I TCNGA 9 cut(s) 245, 285, 382, 517, 793, 949, 1058, 1164, 1279
Hpy188III TCNNGA 6 cut(s) 471, 635, 934, 964, 1247, 1336
Hpy8I GTNNAC 1 cut(s) 187
HpyAV CCTTC 6 cut(s) 286, 378, 686, 1055, 1220, 1253
HpyCH4III ACNGT 2 cut(s) 59, 227
HpyCH4IV ACGT 1 cut(s) 207
HpyCH4V TGCA 4 cut(s) 266, 667, 981, 1085
HpyF10VI GCNNNNNNNGC 2 cut(s) 518, 664
HpyF3I CTNAG 7 cut(s) 63, 242, 282, 792, 918, 923, 1055
HpySE526I ACGT 1 cut(s) 207
Hsp92II CATG 8 cut(s) 149, 240, 526, 779, 816, 989, 1006, 1325
HspAI GCGC 1 cut(s) 532
Kzo9I GATC 5 cut(s) 416, 631, 895, 1005, 1156
LmnI GCTCC 3 cut(s) 83, 248, 596
Lsp1109I GCAGC 2 cut(s) 310, 761
LweI GCATC 2 cut(s) 373, 418
MaeI CTAG 3 cut(s) 527, 1026, 1317
MaeII ACGT 1 cut(s) 207
MaeIII GTNAC 7 cut(s) 160, 442, 487, 615, 778, 926, 1312
MalI GATC 5 cut(s) 418, 633, 897, 1007, 1158
MboI GATC 5 cut(s) 416, 631, 895, 1005, 1156
MboII GAAGA 9 cut(s) 52, 309, 314, 416, 419, 437, 652, 843, 1151
MflI RGATCY 1 cut(s) 1156
MhlI GDGCHC 1 cut(s) 791
MluCI AATT 5 cut(s) 584, 643, 840, 878, 1019
MlyI GAGTC 2 cut(s) 287, 386
MmeI TCCRAC 3 cut(s) 334, 1019, 1238
MroXI GAANNNNTTC 1 cut(s) 644
MseI TTAA 5 cut(s) 102, 261, 738, 1130, 1151
MslI CAYNNNNRTG 1 cut(s) 117
MspCI CTTAAG 1 cut(s) 1129
MspR9I CCNGG 2 cut(s) 79, 1205
Mva1269I GAATGC 2 cut(s) 178, 834
MvaI CCWGG 2 cut(s) 79, 1205
MwoI GCNNNNNNNGC 2 cut(s) 518, 664
NcoI CCATGG 1 cut(s) 1321
NdeI CATATG 1 cut(s) 977
NdeII GATC 5 cut(s) 416, 631, 895, 1005, 1156
NlaIII CATG 8 cut(s) 149, 240, 526, 779, 816, 989, 1006, 1325
NlaIV GGNNCC 1 cut(s) 798
NmuCI GTSAC 4 cut(s) 487, 615, 926, 1312
NspI RCATGY 2 cut(s) 240, 779
PaeR7I CTCGAG 1 cut(s) 471
PciI ACATGT 1 cut(s) 775
PcsI WCGNNNNNNNCGW 1 cut(s) 115
PctI GAATGC 2 cut(s) 178, 834
PdmI GAANNNNTTC 1 cut(s) 644
PfeI GAWTC 7 cut(s) 310, 577, 967, 1106, 1232, 1262, 1274
PflMI CCANNNNNTGG 1 cut(s) 1327
PfoI TCCNGGA 1 cut(s) 1203
PkrI GCNGC 3 cut(s) 325, 751, 1040
PleI GAGTC 2 cut(s) 286, 385
PpsI GAGTC 2 cut(s) 286, 385
PpuMI RGGWCCY 3 cut(s) 796, 1207, 1249
PscI ACATGT 1 cut(s) 775
Psp124BI GAGCTC 1 cut(s) 791
Psp5II RGGWCCY 3 cut(s) 796, 1207, 1249
Psp6I CCWGG 2 cut(s) 77, 1203
PspGI CCWGG 2 cut(s) 77, 1203
PspN4I GGNNCC 1 cut(s) 798
PspPI GGNCC 3 cut(s) 796, 1207, 1249
PspPPI RGGWCCY 3 cut(s) 796, 1207, 1249
PstI CTGCAG 1 cut(s) 669
PsuI RGATCY 1 cut(s) 1156
RsaI GTAC 2 cut(s) 218, 958
RsaNI GTAC 2 cut(s) 217, 957
RseI CAYNNNNRTG 1 cut(s) 117
SacI GAGCTC 1 cut(s) 791
SaqAI TTAA 5 cut(s) 102, 261, 738, 1130, 1151
SatI GCNGC 3 cut(s) 324, 750, 1039
Sau3AI GATC 5 cut(s) 416, 631, 895, 1005, 1156
Sau96I GGNCC 3 cut(s) 796, 1207, 1249
ScaI AGTACT 1 cut(s) 218
SchI GAGTC 2 cut(s) 287, 386
ScrFI CCNGG 2 cut(s) 79, 1205
SduI GDGCHC 1 cut(s) 791
SfaNI GCATC 2 cut(s) 373, 418
SfcI CTRYAG 2 cut(s) 432, 665
Sfr274I CTCGAG 1 cut(s) 471
SinI GGWCC 3 cut(s) 796, 1207, 1249
SlaI CTCGAG 1 cut(s) 471
SmiMI CAYNNNNRTG 1 cut(s) 117
SmlI CTYRAG 8 cut(s) 153, 190, 471, 544, 605, 768, 1129, 1306
SmoI CTYRAG 8 cut(s) 153, 190, 471, 544, 605, 768, 1129, 1306
Sse9I AATT 5 cut(s) 584, 643, 840, 878, 1019
SsiI CCGC 1 cut(s) 1039
SspI AATATT 1 cut(s) 685
SspMI CTAG 3 cut(s) 527, 1026, 1317
SstI GAGCTC 1 cut(s) 791
StyD4I CCNGG 2 cut(s) 77, 1203
StyI CCWWGG 2 cut(s) 198, 1321
TaaI ACNGT 2 cut(s) 59, 227
TaiI ACGT 1 cut(s) 210
TaqI TCGA 1 cut(s) 472
TasI AATT 5 cut(s) 584, 643, 840, 878, 1019
TatI WGTACW 1 cut(s) 216
TauI GCSGC 1 cut(s) 1041
TfiI GAWTC 7 cut(s) 310, 577, 967, 1106, 1232, 1262, 1274
Tru1I TTAA 5 cut(s) 102, 261, 738, 1130, 1151
Tru9I TTAA 5 cut(s) 102, 261, 738, 1130, 1151
TscAI CASTG 3 cut(s) 378, 669, 931
TseFI GTSAC 4 cut(s) 487, 615, 926, 1312
TseI GCWGC 2 cut(s) 323, 749
Tsp45I GTSAC 4 cut(s) 487, 615, 926, 1312
TspDTI ATGAA 2 cut(s) 26, 134
TspGWI ACGGA 2 cut(s) 20, 1001
TspRI CASTG 3 cut(s) 378, 669, 931
Van91I CCANNNNNTGG 1 cut(s) 1327
Vha464I CTTAAG 1 cut(s) 1129
VpaK11BI GGWCC 3 cut(s) 796, 1207, 1249
XapI RAATTY 3 cut(s) 643, 878, 1019
XceI RCATGY 2 cut(s) 240, 779
XhoI CTCGAG 1 cut(s) 471
XmnI GAANNNNTTC 1 cut(s) 644
XspI CTAG 3 cut(s) 527, 1026, 1317
ZrmI AGTACT 1 cut(s) 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.