AT3G45555

protein polyubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000411
Physical Location & Seq
Reverse (-)
16712368 .. 16713009
642 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G45555.1

Sequence Viewer

Length: 642 bp
ATGGAAACAATAGATTGTGAAATCAGCTTACTTGTGGATCCTGAGACTTGCAGTATATGTTTCAATGATGATTTCAAATCTGAGCAAATGTATTATGTTGCTTTATGCAATCATAAATTCTGTTTGGAATGCATGAAACGATATATAGAAGTGAGGCTTCTAGAGGGAACTGTACTGATATGTCCTTATTATCAATGTGAGTCTAAGCTGACTCTTAAAAGTTGTTTTCATATTTTGACATCTAAACTAAAAGCGATGTGGGAACAAAAGATTGAAGAGGAATCCATTCCAGTAACGGAGAGATTTTATTGCCCGAACCCGAGGTGCTCGGCTTTAATGTCGAAAATCGAGCTCTCTAAATCTACCTTAGAAGATGGATTTGTGAGATGCTTCCAATGCGGGGAGCGCTTTTGCATTAACTGCAAAGTTTCATGGCAAAGTAACTTGTCATGTGACAATTGCAAGAAATTGGGTAATAATCCTACATCAGATGATAAGATGCTAAAAGTTCTTGCAAATGAGAAAAAGTGGCGCCAATGTGAAAAGTGCCAACATATGATCAAACTTTCGGAAGGATGCATCCATGTAACTTGCATGTATCTCTCAAATCATTTTCTTAATTATAATCCATTCAAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR RVT_3 zf-C3HC4 zf-RING_2

Protein Analysis

213

Amino Acids

24.96

Weight (kDa)

6.89

Isoelectric Point (pI)

46.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IBR PF01485 90 - 151 3e-10 IBR domain, a half RING-finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000411)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G45470 AT3G45555 AT5G60250
fragaria_vesca FvH4_3g33450 FvH4_3g33450 FvH4_3g33450 FvH4_3g33450 FvH4_3g33450 FvH4_3g33470 FvH4_3g33471 FvH4_3g33480 FvH4_3g33480
malus_domestica MD03G1130800.v1.1 MD11G1125600.v1.1 MD11G1125700.v1.1 MD11G1125800.v1.1 MD11G1126100.v1.1 MD11G1152600.v1.1
prunus_persica Prupe.6G094100_v2.0.a1 Prupe.6G094100_v2.0.a1 Prupe.6G094200_v2.0.a1 Prupe.6G094500_v2.0.a1 Prupe.6G094500_v2.0.a1 Prupe.6G115600_v2.0.a1 Prupe.6G115700_v2.0.a1 Prupe.6G115700_v2.0.a1 Prupe.6G115700_v2.0.a1 Prupe.6G115700_v2.0.a1
pyrus_communis pycom11g10590 pycom11g10600 pycom11g10620 pycom11g10640 pycom11g12510
rosa_chinensis RchiOBHm_Chr5g0060811 RchiOBHm_Chr5g0060821 RchiOBHm_Chr5g0060851 RchiOBHm_Chr5g0060881 RchiOBHm_Chr5g0060891
rosa_laevigata RLG00000035404 RLG00000035405 RLG00000035407 RLG00000035408
rosa_multiflora Rmu_sc0000420.1_g000048 Rmu_sc0000420.1_g000049 Rmu_sc0000727.1_g000006 Rmu_sc0000727.1_g000007 Rmu_sc0000727.1_g000024 Rmu_sc0000727.1_g000025 Rmu_sc0024212.1_g000001 Rmu_sc0024212.1_g000003 Rmu_sc0027900.1_g000003 Rmu_sc0030258.1_g000001
rosa_roxburghii Rroxscaffold_1G00019780 Rroxscaffold_1G00019790 Rroxscaffold_1G00019800 Rroxscaffold_1G00019810 Rroxscaffold_1G00019820 Rroxscaffold_1G00019840 Rroxscaffold_1G00019850
rosa_rugosa Rorug05G0334200 Rorug05G0334300 Rorug05G0334400 Rorug05G0334500 Rorug05G0334600 Rorug05G0334700
rosa_samantha Rh4BG359600 Rh4CG373900 Rh5AG397000 Rh5AG397100 Rh5AG397200 Rh5AG397300 Rh5AG397400 Rh5AG397500 Rh5AG397600 Rh5AG397700 Rh5BG409300 Rh5BG409400 Rh5BG409800 Rh5BG409900 Rh5BG410000 Rh5BG410100 Rh5CG434100 Rh5CG434200 Rh5CG434600 Rh5CG434700 Rh5CG434800 Rh5CG434900 Rh5CG435000 Rh5DG424100 Rh5DG424200 Rh5DG424400 Rh5DG424500 Rh5DG424600 Rh5DG424800
rosa_wichuraiana Rw5G037420 Rw5G037430 Rw5G037480 Rw5G037490 Rw5G037500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 624
AccB1I GGYRCC 1 cut(s) 531
AciI CCGC 1 cut(s) 399
AclWI GGATC 2 cut(s) 32, 45
AcsI RAATTY 2 cut(s) 116, 635
AcyI GRCGYC 1 cut(s) 532
AfaI GTAC 1 cut(s) 174
AfeI AGCGCT 1 cut(s) 407
AfiI CCNNNNNNNGG 1 cut(s) 400
AgsI TTSAA 4 cut(s) 64, 76, 275, 634
AluBI AGCT 3 cut(s) 27, 208, 352
AluI AGCT 3 cut(s) 27, 208, 352
Alw21I GWGCWC 2 cut(s) 329, 354
Alw26I GTCTC 1 cut(s) 38
AlwI GGATC 2 cut(s) 32, 45
Ama87I CYCGRG 1 cut(s) 319
Aor51HI AGCGCT 1 cut(s) 407
ApoI RAATTY 2 cut(s) 116, 635
Asp700I GAANNNNTTC 1 cut(s) 285
AspLEI GCGC 2 cut(s) 408, 534
AvaI CYCGRG 1 cut(s) 319
BamHI GGATCC 1 cut(s) 37
BanI GGYRCC 1 cut(s) 531
BanII GRGCYC 1 cut(s) 354
Bbv12I GWGCWC 2 cut(s) 329, 354
BccI CCATC 1 cut(s) 368
BclI TGATCA 1 cut(s) 558
BcoDI GTCTC 1 cut(s) 38
BfaI CTAG 1 cut(s) 161
BfoI RGCGCY 2 cut(s) 409, 535
BmeT110I CYCGRG 1 cut(s) 319
BmiI GGNNCC 2 cut(s) 39, 533
BmsI GCATC 4 cut(s) 377, 489, 566, 588
BsaHI GRCGYC 1 cut(s) 532
BsaJI CCNNGG 1 cut(s) 320
Bsc4I CCNNNNNNNGG 1 cut(s) 400
Bse1I ACTGG 1 cut(s) 290
BseDI CCNNGG 1 cut(s) 320
BseGI GGATG 2 cut(s) 579, 581
BseLI CCNNNNNNNGG 1 cut(s) 400
BseMII CTCAG 2 cut(s) 33, 72
BseNI ACTGG 1 cut(s) 290
BshNI GGYRCC 1 cut(s) 531
BsiHKAI GWGCWC 2 cut(s) 329, 354
BsiHKCI CYCGRG 1 cut(s) 319
BslI CCNNNNNNNGG 1 cut(s) 400
BsmAI GTCTC 1 cut(s) 38
BsmI GAATGC 1 cut(s) 134
BsoBI CYCGRG 1 cut(s) 319
Bsp1286I GDGCHC 2 cut(s) 329, 354
Bsp143I GATC 2 cut(s) 37, 558
BspACI CCGC 1 cut(s) 399
BspCNI CTCAG 2 cut(s) 34, 73
BspLI GGNNCC 2 cut(s) 39, 533
BspPI GGATC 2 cut(s) 32, 45
BspT107I GGYRCC 1 cut(s) 531
BsrI ACTGG 1 cut(s) 290
BssECI CCNNGG 1 cut(s) 320
BssMI GATC 2 cut(s) 37, 558
BssNI GRCGYC 1 cut(s) 532
Bst4CI ACNGT 1 cut(s) 172
Bst6I CTCTTC 1 cut(s) 270
BstACI GRCGYC 1 cut(s) 532
BstAPI GCANNNNNTGC 1 cut(s) 420
BstDEI CTNAG 4 cut(s) 42, 81, 204, 367
BstF5I GGATG 2 cut(s) 579, 581
BstH2I RGCGCY 2 cut(s) 409, 535
BstHHI GCGC 2 cut(s) 408, 534
BstKTI GATC 2 cut(s) 40, 561
BstMAI GTCTC 1 cut(s) 38
BstMBI GATC 2 cut(s) 37, 558
BstMWI GCNNNNNNNGC 3 cut(s) 396, 405, 420
BstNSI RCATGY 1 cut(s) 598
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
BtgZI GCGATG 1 cut(s) 269
BtsCI GGATG 2 cut(s) 579, 581
CfoI GCGC 2 cut(s) 408, 534
Csp6I GTAC 1 cut(s) 173
CviAII CATG 5 cut(s) 133, 432, 450, 584, 595
CviJI RGCY 5 cut(s) 27, 157, 208, 332, 352
CviKI_1 RGCY 5 cut(s) 27, 157, 208, 332, 352
CviQI GTAC 1 cut(s) 173
DdeI CTNAG 4 cut(s) 42, 81, 204, 367
DinI GGCGCC 1 cut(s) 533
DpnI GATC 2 cut(s) 39, 560
DpnII GATC 2 cut(s) 37, 558
Eam1104I CTCTTC 1 cut(s) 270
EarI CTCTTC 1 cut(s) 270
Ecl136II GAGCTC 1 cut(s) 352
Eco24I GRGCYC 1 cut(s) 354
Eco47III AGCGCT 1 cut(s) 407
Eco53kI GAGCTC 1 cut(s) 352
Eco88I CYCGRG 1 cut(s) 319
EcoICRI GAGCTC 1 cut(s) 352
EcoT22I ATGCAT 2 cut(s) 134, 581
EcoT38I GRGCYC 1 cut(s) 354
EgeI GGCGCC 1 cut(s) 533
EheI GGCGCC 1 cut(s) 533
FaeI CATG 5 cut(s) 136, 435, 453, 587, 598
FalI AAGNNNNNCTT 2 cut(s) 141, 173
FatI CATG 5 cut(s) 132, 431, 449, 583, 594
FauI CCCGC 1 cut(s) 392
FauNDI CATATG 1 cut(s) 555
FbaI TGATCA 1 cut(s) 558
FokI GGATG 2 cut(s) 566, 588
FriOI GRGCYC 1 cut(s) 354
FspBI CTAG 1 cut(s) 161
GlaI GCGC 2 cut(s) 407, 533
HaeII RGCGCY 2 cut(s) 409, 535
HhaI GCGC 2 cut(s) 408, 534
Hin1I GRCGYC 1 cut(s) 532
Hin1II CATG 5 cut(s) 136, 435, 453, 587, 598
Hin6I GCGC 2 cut(s) 406, 532
HinP1I GCGC 2 cut(s) 406, 532
HinfI GANTC 3 cut(s) 200, 211, 281
Hpy188I TCNGA 3 cut(s) 82, 490, 571
Hpy188III TCNNGA 2 cut(s) 41, 161
HpyAV CCTTC 1 cut(s) 566
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4V TGCA 9 cut(s) 51, 108, 132, 414, 423, 462, 515, 579, 594
HpyF10VI GCNNNNNNNGC 3 cut(s) 396, 405, 420
HpyF3I CTNAG 4 cut(s) 42, 81, 204, 367
Hsp92I GRCGYC 1 cut(s) 532
Hsp92II CATG 5 cut(s) 136, 435, 453, 587, 598
HspAI GCGC 2 cut(s) 406, 532
KasI GGCGCC 1 cut(s) 531
Ksp22I TGATCA 1 cut(s) 558
Kzo9I GATC 2 cut(s) 37, 558
LmnI GCTCC 1 cut(s) 403
LpnPI CCDG 2 cut(s) 54, 303
LweI GCATC 4 cut(s) 377, 489, 566, 588
MaeI CTAG 1 cut(s) 161
MaeIII GTNAC 4 cut(s) 292, 440, 452, 586
MalI GATC 2 cut(s) 39, 560
MboI GATC 2 cut(s) 37, 558
MboII GAAGA 2 cut(s) 287, 383
MfeI CAATTG 1 cut(s) 457
MflI RGATCY 1 cut(s) 37
MhlI GDGCHC 2 cut(s) 329, 354
MluCI AATT 5 cut(s) 116, 457, 467, 619, 635
Mly113I GGCGCC 1 cut(s) 532
MlyI GAGTC 2 cut(s) 205, 209
MnlI CCTC 4 cut(s) 147, 157, 271, 315
Mph1103I ATGCAT 2 cut(s) 134, 581
MroXI GAANNNNTTC 1 cut(s) 285
MseI TTAA 4 cut(s) 216, 335, 417, 618
MunI CAATTG 1 cut(s) 457
Mva1269I GAATGC 1 cut(s) 134
MwoI GCNNNNNNNGC 3 cut(s) 396, 405, 420
NarI GGCGCC 1 cut(s) 532
NdeI CATATG 1 cut(s) 555
NdeII GATC 2 cut(s) 37, 558
NlaIII CATG 5 cut(s) 136, 435, 453, 587, 598
NlaIV GGNNCC 2 cut(s) 39, 533
NmeAIII GCCGAG 1 cut(s) 308
NmuCI GTSAC 1 cut(s) 452
NsiI ATGCAT 2 cut(s) 134, 581
NspI RCATGY 1 cut(s) 598
PctI GAATGC 1 cut(s) 134
PdmI GAANNNNTTC 1 cut(s) 285
PfeI GAWTC 1 cut(s) 281
PleI GAGTC 2 cut(s) 205, 208
PluTI GGCGCC 1 cut(s) 535
PpsI GAGTC 2 cut(s) 205, 208
PsiI TTATAA 1 cut(s) 624
Psp124BI GAGCTC 1 cut(s) 354
PspN4I GGNNCC 2 cut(s) 39, 533
PsuI RGATCY 1 cut(s) 37
RsaI GTAC 1 cut(s) 174
RsaNI GTAC 1 cut(s) 173
SacI GAGCTC 1 cut(s) 354
SaqAI TTAA 4 cut(s) 216, 335, 417, 618
Sau3AI GATC 2 cut(s) 37, 558
SchI GAGTC 2 cut(s) 205, 209
SduI GDGCHC 2 cut(s) 329, 354
SetI ASST 5 cut(s) 29, 210, 326, 354, 368
SfaNI GCATC 4 cut(s) 377, 489, 566, 588
SfoI GGCGCC 1 cut(s) 533
Sse9I AATT 5 cut(s) 116, 457, 467, 619, 635
SsiI CCGC 1 cut(s) 399
SspDI GGCGCC 1 cut(s) 531
SspMI CTAG 1 cut(s) 161
SstI GAGCTC 1 cut(s) 354
TaaI ACNGT 1 cut(s) 172
TaqI TCGA 2 cut(s) 341, 348
TasI AATT 5 cut(s) 116, 457, 467, 619, 635
TatI WGTACW 1 cut(s) 172
TfiI GAWTC 1 cut(s) 281
Tru1I TTAA 4 cut(s) 216, 335, 417, 618
Tru9I TTAA 4 cut(s) 216, 335, 417, 618
TseFI GTSAC 1 cut(s) 452
Tsp45I GTSAC 1 cut(s) 452
TspDTI ATGAA 3 cut(s) 149, 218, 420
TspGWI ACGGA 1 cut(s) 311
XapI RAATTY 2 cut(s) 116, 635
XbaI TCTAGA 1 cut(s) 160
XceI RCATGY 1 cut(s) 598
XmnI GAANNNNTTC 1 cut(s) 285
XspI CTAG 1 cut(s) 161
Zsp2I ATGCAT 2 cut(s) 134, 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.