AT5G60250

Reverse transcriptase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000411
Physical Location & Seq
Forward (+)
24252138 .. 24255900
3763 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G60250.1

Sequence Viewer

Length: 1968 bp
ATGGATTTCGATTCCGAGTATGCTTTTCGGTTACAGATGGAGGAGGCACTCGCGGCTTCTCTATCTTCTCAATCTCGCACTCCGCAACGACCACCGTCACCTCCGATTGTGGCTACGTGTGGTGTCCTTATCGTTGAAAACAATCCAAACGATTCGAAGACGGCGAAAACAAGTAGCGGTTACGGTTTCGATTTTAAGAGAGCCGTCGGCGGTGGAAACTCTAAAGCAAAAGGCAAAACCCACGAGTCTGTCACTGGTGTACGTACAGATGAACGAAACCCTAACATTGGGCTCGGTAACTCCCGGAGTACGTCTACCGGACATGGCAATACGAAACCCTTGCCTCAGGAAACTGTTCGTCCGGCGCAATTAGTCGGTGAGGGAAGTTCGAGGGTTAACACTGCTGCCGGAAAAATCAATGGAAACCTCTTGTATAGGCTTTACTTTAAGGGTTTGGTTAGCGATGAGAACGGGAAGGGGAAGATGACGGACGTAGTGTCTGGGTTTGGGGTTGCTATTTGTGACCAGAGGGATAATTTGTTGTTTGAGATGAAGGGACCATTGATAGACAATGGCATGAGTCGTCAGGGAGCAGAGCTTAAGGCATTGATCCGAGGACTAACCGAAGCATTGAAGTTGGGGATTAAGCATATTGTTTTCTTTTGCGATTCTTATCCGATTTTCCAATACGTCACGGGAAAATGGATGGCTAAGCAAAAGAAGATTTCCCTGTTGTTAGATGATCTTCAAAGCATCATGCAACACTTTTCATCTTATCAGCATGTTCTGGTGGCTAGAAATGATGTTAAATTTGCTTATAAACTTGCAAGGGAATCAATACTTTCTATGGTCACCCCTCATGAAGATCCTCGCCAAGCAAAAGCTGTTCTGAAAGAGGAGTGTGCTATCTGTTTCAACGACATCGTTGCTGAGGGTATGTTTTCTGTTGACAAATGCCGTCACCGTTTTTGCTTTCAATGTGTGAAACAGCATGTAGAGGTGAAGCTGCTTCATGGAATGGCTCCAAAATGTCCTCACGATGGCTGCAAGTCTGAGCTGGTTATCGATGCATGTGGCAAGCTTTTGACTCCAAAGCTGAGTAAATTGTGGCAACAAAGACTTCAAGAGAATGCAATACCTGTCACAGAGAGAGTTTACTGCCCTTACCCGAGGTGCTCGGCTTTGATGTCCAAAACCAAGATTTCTGAATCTGCCAAGAGCTTACTGTCTCTTTACCCTAAATCTGGAGTTAGGAGATGTGTTGAATGTCGTGGCCTCTTCTGTGTGGACTGTAAAGTTCCGTGGCATGGAAATCTGTCCTGCACTGAGTATAAGAAATTGCATCCTGAGCCTCCGGCAGATGATGTGAAGCTCAAATCTCTTGCAAATAATAAGATGTGGCGCCAGTGTGGCAAGTGCCAACACATGATTGAACTTTCACAAGGATGCAATCACATAACTTGCAGATGCGGGCATGAGTTTTGCTACAATTGCGGAGGTGGATGGAACAAGATAATGGGGACTTGTTTAAATCGCTGCCCAACCTGGAACGAAGAATACATCACACGACAAGATCCAGCGCGTGCCAATGTGGCAGCTAACAACTACTTTGATGATGAGGATGATGATGATGTTGATGGTGATTACGACTATGATGACTTCTTCCACCCGCATTTTGGACATGCTATGAATGATCGTAATCCTGAAGAGCCCTTCGACCCCTTCTTCGACTTGCCAGATGGTGTGCTCTTTACCCCCCAGATGTTGTCTTCCAAGAGTCGTCAGCAATTGTACTTGCCGGATTCAGATGATTCAGATGATAATGCGACAACACAAAATGCTCCTGCTGCAGATGAGAATTGCCCATACTTTGGGAAGTATGGAAAGTACACTATAATGTACGACTCCGACGGCTATGAACTCGAGGATTTCACGAACCCTTTCCATCCTGACTATCCTCACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR RVT_3 zf-C3HC4

Protein Analysis

655

Amino Acids

73.47

Weight (kDa)

6.38

Isoelectric Point (pI)

40.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 159 - 278 2.1e-32 Reverse transcriptase-like
IBR PF01485 373 - 441 1.2e-10 IBR domain, a half RING-finger domain
IBR PF01485 457 - 509 1.7e-08 IBR domain, a half RING-finger domain
IBR_1 PF22191 469 - 503 3.3e-07 IBR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000411)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G45470 AT3G45555 AT5G60250
fragaria_vesca FvH4_3g33450 FvH4_3g33450 FvH4_3g33450 FvH4_3g33450 FvH4_3g33450 FvH4_3g33470 FvH4_3g33471 FvH4_3g33480 FvH4_3g33480
malus_domestica MD03G1130800.v1.1 MD11G1125600.v1.1 MD11G1125700.v1.1 MD11G1125800.v1.1 MD11G1126100.v1.1 MD11G1152600.v1.1
prunus_persica Prupe.6G094100_v2.0.a1 Prupe.6G094100_v2.0.a1 Prupe.6G094200_v2.0.a1 Prupe.6G094500_v2.0.a1 Prupe.6G094500_v2.0.a1 Prupe.6G115600_v2.0.a1 Prupe.6G115700_v2.0.a1 Prupe.6G115700_v2.0.a1 Prupe.6G115700_v2.0.a1 Prupe.6G115700_v2.0.a1
pyrus_communis pycom11g10590 pycom11g10600 pycom11g10620 pycom11g10640 pycom11g12510
rosa_chinensis RchiOBHm_Chr5g0060811 RchiOBHm_Chr5g0060821 RchiOBHm_Chr5g0060851 RchiOBHm_Chr5g0060881 RchiOBHm_Chr5g0060891
rosa_laevigata RLG00000035404 RLG00000035405 RLG00000035407 RLG00000035408
rosa_multiflora Rmu_sc0000420.1_g000048 Rmu_sc0000420.1_g000049 Rmu_sc0000727.1_g000006 Rmu_sc0000727.1_g000007 Rmu_sc0000727.1_g000024 Rmu_sc0000727.1_g000025 Rmu_sc0024212.1_g000001 Rmu_sc0024212.1_g000003 Rmu_sc0027900.1_g000003 Rmu_sc0030258.1_g000001
rosa_roxburghii Rroxscaffold_1G00019780 Rroxscaffold_1G00019790 Rroxscaffold_1G00019800 Rroxscaffold_1G00019810 Rroxscaffold_1G00019820 Rroxscaffold_1G00019840 Rroxscaffold_1G00019850
rosa_rugosa Rorug05G0334200 Rorug05G0334300 Rorug05G0334400 Rorug05G0334500 Rorug05G0334600 Rorug05G0334700
rosa_samantha Rh4BG359600 Rh4CG373900 Rh5AG397000 Rh5AG397100 Rh5AG397200 Rh5AG397300 Rh5AG397400 Rh5AG397500 Rh5AG397600 Rh5AG397700 Rh5BG409300 Rh5BG409400 Rh5BG409800 Rh5BG409900 Rh5BG410000 Rh5BG410100 Rh5CG434100 Rh5CG434200 Rh5CG434600 Rh5CG434700 Rh5CG434800 Rh5CG434900 Rh5CG435000 Rh5DG424100 Rh5DG424200 Rh5DG424400 Rh5DG424500 Rh5DG424600 Rh5DG424800
rosa_wichuraiana Rw5G037420 Rw5G037430 Rw5G037480 Rw5G037490 Rw5G037500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 819
AccB1I GGYRCC 1 cut(s) 1401
AccB7I CCANNNNNTGG 1 cut(s) 1871
AccI GTMKAC 1 cut(s) 314
AccII CGCG 2 cut(s) 53, 1582
AciI CCGC 7 cut(s) 53, 83, 177, 210, 1470, 1494, 1670
AclWI GGATC 3 cut(s) 604, 860, 1568
AcsI RAATTY 1 cut(s) 809
AcuI CTGAAG 1 cut(s) 1725
AcyI GRCGYC 1 cut(s) 1402
AfaI GTAC 6 cut(s) 261, 265, 310, 1793, 1889, 1901
AfiI CCNNNNNNNGG 6 cut(s) 287, 1040, 1244, 1307, 1676, 1871
AflII CTTAAG 1 cut(s) 599
AflIII ACRYGT 1 cut(s) 116
AgsI TTSAA 8 cut(s) 137, 634, 749, 916, 977, 1124, 1265, 1433
AhdI GACNNNNNGTC 1 cut(s) 496
AjnI CCWGG 1 cut(s) 1544
AluBI AGCT 9 cut(s) 598, 884, 1006, 1057, 1081, 1096, 1221, 1372, 1598
AluI AGCT 9 cut(s) 598, 884, 1006, 1057, 1081, 1096, 1221, 1372, 1598
Alw21I GWGCWC 2 cut(s) 1178, 1749
Alw26I GTCTC 1 cut(s) 1233
AlwI GGATC 3 cut(s) 604, 860, 1568
Ama87I CYCGRG 2 cut(s) 1168, 1922
AoxI GGCC 1 cut(s) 1273
ApeKI GCWGC 6 cut(s) 404, 1006, 1044, 1536, 1595, 1847
ApoI RAATTY 1 cut(s) 809
ArsI GACNNNNNNTTYG 2 cut(s) 1709, 1741
Asp700I GAANNNNTTC 2 cut(s) 354, 1940
AspLEI GCGC 3 cut(s) 367, 1404, 1582
AspS9I GGNCC 1 cut(s) 557
AsuC2I CCSGG 1 cut(s) 304
AsuHPI GGTGA 6 cut(s) 90, 389, 844, 953, 1012, 1652
AsuII TTCGAA 1 cut(s) 155
AvaI CYCGRG 2 cut(s) 1168, 1922
AvaII GGWCC 1 cut(s) 557
AxyI CCTNAGG 1 cut(s) 345
BanI GGYRCC 1 cut(s) 1401
BanII GRGCYC 2 cut(s) 294, 1713
BauI CACGAG 1 cut(s) 242
BbsI GAAGAC 2 cut(s) 164, 1761
Bbv12I GWGCWC 2 cut(s) 1178, 1749
BbvCI CCTCAGC 1 cut(s) 930
BbvI GCAGC 6 cut(s) 391, 993, 1031, 1523, 1607, 1834
BccI CCATC 7 cut(s) 31, 700, 1034, 1497, 1631, 1733, 1953
BceAI ACGGC 4 cut(s) 177, 188, 942, 1927
BcgI CGANNNNNNTGC 4 cut(s) 322, 356, 908, 942
BciT130I CCWGG 1 cut(s) 1546
BcnI CCSGG 1 cut(s) 304
BcoDI GTCTC 1 cut(s) 1233
BfaI CTAG 1 cut(s) 795
BfmI CTRYAG 1 cut(s) 1848
BfoI RGCGCY 1 cut(s) 1405
BfrI CTTAAG 1 cut(s) 599
BglI GCCNNNNNGGC 2 cut(s) 1410, 1592
BisI GCNGC 7 cut(s) 54, 405, 1007, 1045, 1537, 1596, 1848
BlpI GCTNAGC 1 cut(s) 711
BlsI GCNGC 7 cut(s) 55, 406, 1008, 1046, 1538, 1597, 1849
Bme1390I CCNGG 2 cut(s) 304, 1546
Bme18I GGWCC 1 cut(s) 557
BmeRI GACNNNNNGTC 1 cut(s) 496
BmeT110I CYCGRG 2 cut(s) 1168, 1922
BmgT120I GGNCC 1 cut(s) 557
BmiI GGNNCC 3 cut(s) 558, 1023, 1403
BmrFI CCNGG 2 cut(s) 304, 1546
BmsI GCATC 5 cut(s) 762, 1057, 1351, 1436, 1457
BoxI GACNNNNGTC 1 cut(s) 94
BpiI GAAGAC 2 cut(s) 164, 1761
BpmI CTGGAG 1 cut(s) 1266
Bpu10I CCTNAGC 2 cut(s) 930, 1347
Bpu1102I GCTNAGC 1 cut(s) 711
Bpu14I TTCGAA 1 cut(s) 155
BpuMI CCSGG 1 cut(s) 304
Bsa29I ATCGAT 1 cut(s) 1065
BsaAI YACGTR 2 cut(s) 117, 263
BsaBI GATNNNNATC 2 cut(s) 672, 1698
BsaHI GRCGYC 1 cut(s) 1402
BsaJI CCNNGG 3 cut(s) 613, 1169, 1301
BsaWI WCCGGW 1 cut(s) 317
Bsc4I CCNNNNNNNGG 6 cut(s) 287, 1040, 1244, 1307, 1676, 1871
Bse1I ACTGG 2 cut(s) 259, 1405
Bse21I CCTNAGG 1 cut(s) 345
Bse8I GATNNNNATC 2 cut(s) 672, 1698
BseBI CCWGG 1 cut(s) 1546
BseCI ATCGAT 1 cut(s) 1065
BseDI CCNNGG 3 cut(s) 613, 1169, 1301
BseGI GGATG 6 cut(s) 711, 1342, 1451, 1508, 1627, 1945
BseJI GATNNNNATC 2 cut(s) 672, 1698
BseLI CCNNNNNNNGG 6 cut(s) 287, 1040, 1244, 1307, 1676, 1871
BseMII CTCAG 6 cut(s) 359, 921, 1044, 1088, 1317, 1338
BseNI ACTGG 2 cut(s) 259, 1405
BseRI GAGGAG 2 cut(s) 56, 911
BseXI GCAGC 6 cut(s) 391, 993, 1031, 1523, 1607, 1834
BsgI GTGCAG 1 cut(s) 1306
Bsh1236I CGCG 2 cut(s) 53, 1582
BshFI GGCC 1 cut(s) 1275
BshNI GGYRCC 1 cut(s) 1401
BshVI ATCGAT 1 cut(s) 1065
BsiHKAI GWGCWC 2 cut(s) 1178, 1749
BsiHKCI CYCGRG 2 cut(s) 1168, 1922
BsiSI CCGG 6 cut(s) 304, 318, 362, 408, 1355, 1799
BslFI GGGAC 2 cut(s) 570, 1534
BslI CCNNNNNNNGG 6 cut(s) 287, 1040, 1244, 1307, 1676, 1871
BsmAI GTCTC 1 cut(s) 1233
BsmFI GGGAC 2 cut(s) 570, 1534
BsmI GAATGC 1 cut(s) 1135
BsnI GGCC 1 cut(s) 1275
BsoBI CYCGRG 2 cut(s) 1168, 1922
Bsp119I TTCGAA 1 cut(s) 155
Bsp1286I GDGCHC 4 cut(s) 294, 1178, 1713, 1749
Bsp143I GATC 5 cut(s) 609, 742, 865, 1573, 1693
Bsp1720I GCTNAGC 1 cut(s) 711
BspACI CCGC 7 cut(s) 53, 83, 177, 210, 1470, 1494, 1670
BspANI GGCC 1 cut(s) 1275
BspCNI CTCAG 6 cut(s) 358, 922, 1045, 1089, 1318, 1339
BspDI ATCGAT 1 cut(s) 1065
BspFNI CGCG 2 cut(s) 53, 1582
BspHI TCATGA 1 cut(s) 859
BspLI GGNNCC 3 cut(s) 558, 1023, 1403
BspMAI CTGCAG 1 cut(s) 1852
BspPI GGATC 3 cut(s) 604, 860, 1568
BspQI GCTCTTC 1 cut(s) 1701
BspT104I TTCGAA 1 cut(s) 155
BspT107I GGYRCC 1 cut(s) 1401
BspTI CTTAAG 1 cut(s) 599
BsrI ACTGG 2 cut(s) 259, 1405
BssECI CCNNGG 3 cut(s) 613, 1169, 1301
BssMI GATC 5 cut(s) 609, 742, 865, 1573, 1693
BssNI GRCGYC 1 cut(s) 1402
BssSI CACGAG 1 cut(s) 242
Bst2BI CACGAG 1 cut(s) 242
Bst2UI CCWGG 1 cut(s) 1546
Bst4CI ACNGT 6 cut(s) 96, 185, 355, 965, 1227, 1292
Bst6I CTCTTC 2 cut(s) 1283, 1701
BstACI GRCGYC 1 cut(s) 1402
BstAFI CTTAAG 1 cut(s) 599
BstBAI YACGTR 2 cut(s) 117, 263
BstBI TTCGAA 1 cut(s) 155
BstC8I GCNNGC 3 cut(s) 1079, 1472, 1584
BstDEI CTNAG 7 cut(s) 345, 711, 930, 1053, 1097, 1326, 1347
BstDSI CCRYGG 1 cut(s) 1301
BstEII GGTNACC 1 cut(s) 850
BstF5I GGATG 6 cut(s) 711, 1342, 1451, 1508, 1627, 1945
BstFNI CGCG 2 cut(s) 53, 1582
BstH2I RGCGCY 1 cut(s) 1405
BstHHI GCGC 3 cut(s) 367, 1404, 1582
BstKTI GATC 5 cut(s) 612, 745, 868, 1576, 1696
BstMAI GTCTC 1 cut(s) 1233
BstMBI GATC 5 cut(s) 609, 742, 865, 1573, 1693
BstMWI GCNNNNNNNGC 6 cut(s) 53, 1348, 1410, 1491, 1592, 1847
BstNI CCWGG 1 cut(s) 1546
BstNSI RCATGY 4 cut(s) 785, 995, 1074, 1685
BstPAI GACNNNNGTC 1 cut(s) 94
BstPI GGTNACC 1 cut(s) 850
BstSCI CCNGG 2 cut(s) 302, 1544
BstSFI CTRYAG 1 cut(s) 1848
BstSNI TACGTA 1 cut(s) 263
BstUI CGCG 2 cut(s) 53, 1582
BstV1I GCAGC 6 cut(s) 391, 993, 1031, 1523, 1607, 1834
BstV2I GAAGAC 2 cut(s) 164, 1761
BstX2I RGATCY 2 cut(s) 865, 1573
BstYI RGATCY 2 cut(s) 865, 1573
Bsu15I ATCGAT 1 cut(s) 1065
Bsu36I CCTNAGG 1 cut(s) 345
BsuRI GGCC 1 cut(s) 1275
BsuTUI ATCGAT 1 cut(s) 1065
BtgI CCRYGG 1 cut(s) 1301
BtgZI GCGATG 1 cut(s) 477
BtsCI GGATG 6 cut(s) 711, 1342, 1451, 1508, 1627, 1945
BtsI GCAGTG 1 cut(s) 399
BtsIMutI CAGTG 4 cut(s) 252, 399, 1323, 1412
Cac8I GCNNGC 3 cut(s) 1079, 1472, 1584
CciI TCATGA 1 cut(s) 859
CfoI GCGC 3 cut(s) 367, 1404, 1582
Cfr13I GGNCC 1 cut(s) 557
ClaI ATCGAT 1 cut(s) 1065
Csp6I GTAC 6 cut(s) 260, 264, 309, 1792, 1888, 1900
CviQI GTAC 6 cut(s) 260, 264, 309, 1792, 1888, 1900
DdeI CTNAG 7 cut(s) 345, 711, 930, 1053, 1097, 1326, 1347
DinI GGCGCC 1 cut(s) 1403
DpnI GATC 5 cut(s) 611, 744, 867, 1575, 1695
DpnII GATC 5 cut(s) 609, 742, 865, 1573, 1693
DraI TTTAAA 1 cut(s) 1530
DriI GACNNNNNGTC 1 cut(s) 496
Eam1104I CTCTTC 2 cut(s) 1283, 1701
Eam1105I GACNNNNNGTC 1 cut(s) 496
EarI CTCTTC 2 cut(s) 1283, 1701
Eco105I TACGTA 1 cut(s) 263
Eco24I GRGCYC 2 cut(s) 294, 1713
Eco47I GGWCC 1 cut(s) 557
Eco57I CTGAAG 1 cut(s) 1725
Eco81I CCTNAGG 1 cut(s) 345
Eco88I CYCGRG 2 cut(s) 1168, 1922
Eco91I GGTNACC 1 cut(s) 850
EcoO65I GGTNACC 1 cut(s) 850
EcoRII CCWGG 1 cut(s) 1544
EcoT22I ATGCAT 1 cut(s) 1072
EcoT38I GRGCYC 2 cut(s) 294, 1713
EgeI GGCGCC 1 cut(s) 1403
EheI GGCGCC 1 cut(s) 1403
FaqI GGGAC 2 cut(s) 570, 1534
FauI CCCGC 2 cut(s) 1463, 1677
FblI GTMKAC 1 cut(s) 314
Fnu4HI GCNGC 7 cut(s) 54, 405, 1007, 1045, 1537, 1596, 1848
FokI GGATG 6 cut(s) 718, 1329, 1458, 1515, 1634, 1932
FriOI GRGCYC 2 cut(s) 294, 1713
Fsp4HI GCNGC 7 cut(s) 54, 405, 1007, 1045, 1537, 1596, 1848
FspBI CTAG 1 cut(s) 795
GlaI GCGC 3 cut(s) 366, 1403, 1581
GluI GCNGC 7 cut(s) 54, 405, 1007, 1045, 1537, 1596, 1848
GsuI CTGGAG 1 cut(s) 1266
HaeII RGCGCY 1 cut(s) 1405
HaeIII GGCC 1 cut(s) 1275
HapII CCGG 6 cut(s) 304, 318, 362, 408, 1355, 1799
HhaI GCGC 3 cut(s) 367, 1404, 1582
Hin1I GRCGYC 1 cut(s) 1402
Hin6I GCGC 3 cut(s) 365, 1402, 1580
HinP1I GCGC 3 cut(s) 365, 1402, 1580
HincII GTYRAC 2 cut(s) 397, 949
HindII GTYRAC 2 cut(s) 397, 949
HindIII AAGCTT 1 cut(s) 1079
HpaI GTTAAC 1 cut(s) 397
HpaII CCGG 6 cut(s) 304, 318, 362, 408, 1355, 1799
HphI GGTGA 6 cut(s) 90, 389, 844, 953, 1012, 1652
Hpy166II GTNNAC 7 cut(s) 260, 315, 397, 949, 1156, 1288, 1890
Hpy188III TCNNGA 9 cut(s) 347, 860, 1037, 1124, 1245, 1346, 1703, 1933, 1949
Hpy8I GTNNAC 7 cut(s) 260, 315, 397, 949, 1156, 1288, 1890
Hpy99I CGWCG 2 cut(s) 209, 1913
HpyAV CCTTC 4 cut(s) 469, 547, 1723, 1732
HpyCH4III ACNGT 6 cut(s) 96, 185, 355, 965, 1227, 1292
HpyCH4IV ACGT 5 cut(s) 116, 262, 311, 492, 690
HpyF10VI GCNNNNNNNGC 6 cut(s) 53, 1348, 1410, 1491, 1592, 1847
HpyF3I CTNAG 7 cut(s) 345, 711, 930, 1053, 1097, 1326, 1347
HpySE526I ACGT 5 cut(s) 116, 262, 311, 492, 690
Hsp92I GRCGYC 1 cut(s) 1402
HspAI GCGC 3 cut(s) 365, 1402, 1580
KasI GGCGCC 1 cut(s) 1401
KspAI GTTAAC 1 cut(s) 397
Kzo9I GATC 5 cut(s) 609, 742, 865, 1573, 1693
LguI GCTCTTC 1 cut(s) 1701
LmnI GCTCC 3 cut(s) 590, 1027, 1846
Lsp1109I GCAGC 6 cut(s) 391, 993, 1031, 1523, 1607, 1834
LweI GCATC 5 cut(s) 762, 1057, 1351, 1436, 1457
MaeI CTAG 1 cut(s) 795
MaeII ACGT 5 cut(s) 116, 262, 311, 492, 690
MalI GATC 5 cut(s) 611, 744, 867, 1575, 1695
MboI GATC 5 cut(s) 609, 742, 865, 1573, 1693
MfeI CAATTG 2 cut(s) 1489, 1787
MflI RGATCY 2 cut(s) 865, 1573
MhlI GDGCHC 4 cut(s) 294, 1178, 1713, 1749
MluCI AATT 8 cut(s) 368, 535, 809, 1103, 1337, 1489, 1787, 1858
Mly113I GGCGCC 1 cut(s) 1402
MlyI GAGTC 5 cut(s) 254, 589, 1081, 1786, 1898
MmeI TCCRAC 1 cut(s) 1932
Mph1103I ATGCAT 1 cut(s) 1072
MroXI GAANNNNTTC 2 cut(s) 354, 1940
MseI TTAA 7 cut(s) 195, 396, 447, 600, 645, 807, 1529
MslI CAYNNNNRTG 2 cut(s) 1444, 1895
MspCI CTTAAG 1 cut(s) 599
MspI CCGG 6 cut(s) 304, 318, 362, 408, 1355, 1799
MspR9I CCNGG 2 cut(s) 304, 1546
MunI CAATTG 2 cut(s) 1489, 1787
Mva1269I GAATGC 1 cut(s) 1135
MvaI CCWGG 1 cut(s) 1546
MvnI CGCG 2 cut(s) 53, 1582
MwoI GCNNNNNNNGC 6 cut(s) 53, 1348, 1410, 1491, 1592, 1847
NarI GGCGCC 1 cut(s) 1402
NciI CCSGG 1 cut(s) 304
NdeII GATC 5 cut(s) 609, 742, 865, 1573, 1693
NlaIV GGNNCC 3 cut(s) 558, 1023, 1403
NmeAIII GCCGAG 1 cut(s) 1157
NmuCI GTSAC 7 cut(s) 96, 250, 521, 691, 850, 959, 1141
NsiI ATGCAT 1 cut(s) 1072
NspI RCATGY 4 cut(s) 785, 995, 1074, 1685
NspV TTCGAA 1 cut(s) 155
PaeR7I CTCGAG 1 cut(s) 1922
PagI TCATGA 1 cut(s) 859
PciSI GCTCTTC 1 cut(s) 1701
PcsI WCGNNNNNNNCGW 1 cut(s) 161
PctI GAATGC 1 cut(s) 1135
PdmI GAANNNNTTC 2 cut(s) 354, 1940
PfeI GAWTC 7 cut(s) 11, 152, 668, 833, 1208, 1802, 1811
PflMI CCANNNNNTGG 1 cut(s) 1871
PfoI TCCNGGA 1 cut(s) 302
PkrI GCNGC 7 cut(s) 55, 406, 1008, 1046, 1538, 1597, 1849
PleI GAGTC 5 cut(s) 253, 588, 1081, 1785, 1898
PluTI GGCGCC 1 cut(s) 1405
PpsI GAGTC 5 cut(s) 253, 588, 1081, 1785, 1898
Ppu21I YACGTR 2 cut(s) 117, 263
PshAI GACNNNNGTC 1 cut(s) 94
PsiI TTATAA 1 cut(s) 819
Psp6I CCWGG 1 cut(s) 1544
PspEI GGTNACC 1 cut(s) 850
PspGI CCWGG 1 cut(s) 1544
PspN4I GGNNCC 3 cut(s) 558, 1023, 1403
PspPI GGNCC 1 cut(s) 557
PspXI VCTCGAGB 1 cut(s) 1922
PsrI GAACNNNNNNTAC 2 cut(s) 1541, 1573
PstI CTGCAG 1 cut(s) 1852
PsuI RGATCY 2 cut(s) 865, 1573
RsaI GTAC 6 cut(s) 261, 265, 310, 1793, 1889, 1901
RsaNI GTAC 6 cut(s) 260, 264, 309, 1792, 1888, 1900
RseI CAYNNNNRTG 2 cut(s) 1444, 1895
SapI GCTCTTC 1 cut(s) 1701
SaqAI TTAA 7 cut(s) 195, 396, 447, 600, 645, 807, 1529
SatI GCNGC 7 cut(s) 54, 405, 1007, 1045, 1537, 1596, 1848
Sau3AI GATC 5 cut(s) 609, 742, 865, 1573, 1693
Sau96I GGNCC 1 cut(s) 557
SchI GAGTC 5 cut(s) 254, 589, 1081, 1786, 1898
ScrFI CCNGG 2 cut(s) 304, 1546
SduI GDGCHC 4 cut(s) 294, 1178, 1713, 1749
SfaNI GCATC 5 cut(s) 762, 1057, 1351, 1436, 1457
SfcI CTRYAG 1 cut(s) 1848
SfoI GGCGCC 1 cut(s) 1403
Sfr274I CTCGAG 1 cut(s) 1922
SfuI TTCGAA 1 cut(s) 155
SinI GGWCC 1 cut(s) 557
SlaI CTCGAG 1 cut(s) 1922
SmiMI CAYNNNNRTG 2 cut(s) 1444, 1895
SmlI CTYRAG 2 cut(s) 599, 1922
SmoI CTYRAG 2 cut(s) 599, 1922
SnaBI TACGTA 1 cut(s) 263
Sse9I AATT 8 cut(s) 368, 535, 809, 1103, 1337, 1489, 1787, 1858
SsiI CCGC 7 cut(s) 53, 83, 177, 210, 1470, 1494, 1670
SspDI GGCGCC 1 cut(s) 1401
SspMI CTAG 1 cut(s) 795
StyD4I CCNGG 2 cut(s) 302, 1544
TaaI ACNGT 6 cut(s) 96, 185, 355, 965, 1227, 1292
TaiI ACGT 5 cut(s) 119, 265, 314, 495, 693
TaqI TCGA 8 cut(s) 9, 155, 189, 389, 1065, 1716, 1728, 1923
TasI AATT 8 cut(s) 368, 535, 809, 1103, 1337, 1489, 1787, 1858
TatI WGTACW 2 cut(s) 1791, 1887
TauI GCSGC 1 cut(s) 56
TfiI GAWTC 7 cut(s) 11, 152, 668, 833, 1208, 1802, 1811
Tru1I TTAA 7 cut(s) 195, 396, 447, 600, 645, 807, 1529
Tru9I TTAA 7 cut(s) 195, 396, 447, 600, 645, 807, 1529
TscAI CASTG 4 cut(s) 259, 406, 1330, 1412
TseFI GTSAC 7 cut(s) 96, 250, 521, 691, 850, 959, 1141
TseI GCWGC 6 cut(s) 404, 1006, 1044, 1536, 1595, 1847
Tsp45I GTSAC 7 cut(s) 96, 250, 521, 691, 850, 959, 1141
TspDTI ATGAA 7 cut(s) 285, 566, 759, 876, 1001, 1703, 1932
TspGWI ACGGA 2 cut(s) 503, 1290
TspRI CASTG 4 cut(s) 259, 406, 1330, 1412
Van91I CCANNNNNTGG 1 cut(s) 1871
Vha464I CTTAAG 1 cut(s) 599
VpaK11BI GGWCC 1 cut(s) 557
XapI RAATTY 1 cut(s) 809
XceI RCATGY 4 cut(s) 785, 995, 1074, 1685
XcmI CCANNNNNNNNNTGG 1 cut(s) 1673
XhoI CTCGAG 1 cut(s) 1922
XmiI GTMKAC 1 cut(s) 314
XmnI GAANNNNTTC 2 cut(s) 354, 1940
XspI CTAG 1 cut(s) 795
Zsp2I ATGCAT 1 cut(s) 1072
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.