E3 ubiquitin-protein ligase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 885 |
| Acc36I | ACCTGC | 2 cut(s) | 764, 885 |
| AccB1I | GGYRCC | 1 cut(s) | 831 |
| AcoI | YGGCCR | 3 cut(s) | 15, 30, 305 |
| AcsI | RAATTY | 1 cut(s) | 212 |
| AcuI | CTGAAG | 1 cut(s) | 513 |
| AcyI | GRCGYC | 1 cut(s) | 832 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 24, 479 |
| AgsI | TTSAA | 9 cut(s) | 143, 169, 218, 269, 356, 384, 437, 542, 863 |
| AluBI | AGCT | 3 cut(s) | 127, 311, 510 |
| AluI | AGCT | 3 cut(s) | 127, 311, 510 |
| Alw26I | GTCTC | 1 cut(s) | 329 |
| AoxI | GGCC | 4 cut(s) | 15, 30, 66, 305 |
| ApeKI | GCWGC | 3 cut(s) | 119, 225, 849 |
| ApoI | RAATTY | 1 cut(s) | 212 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 242, 274 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 379 |
| AspA2I | CCTAGG | 1 cut(s) | 245 |
| AspLEI | GCGC | 2 cut(s) | 552, 834 |
| AsuC2I | CCSGG | 1 cut(s) | 19 |
| AsuHPI | GGTGA | 2 cut(s) | 110, 814 |
| AsuNHI | GCTAGC | 1 cut(s) | 311 |
| AvrII | CCTAGG | 1 cut(s) | 245 |
| BalI | TGGCCA | 2 cut(s) | 32, 307 |
| BanI | GGYRCC | 1 cut(s) | 831 |
| BanII | GRGCYC | 1 cut(s) | 150 |
| BbvI | GCAGC | 3 cut(s) | 106, 237, 861 |
| BceAI | ACGGC | 1 cut(s) | 406 |
| BcnI | CCSGG | 1 cut(s) | 19 |
| BcoDI | GTCTC | 1 cut(s) | 329 |
| BfaI | CTAG | 4 cut(s) | 45, 246, 312, 372 |
| BfmI | CTRYAG | 2 cut(s) | 285, 847 |
| BfoI | RGCGCY | 1 cut(s) | 835 |
| BfuAI | ACCTGC | 2 cut(s) | 764, 885 |
| BisI | GCNGC | 3 cut(s) | 120, 226, 850 |
| BlnI | CCTAGG | 1 cut(s) | 245 |
| BlpI | GCTNAGC | 1 cut(s) | 123 |
| BlsI | GCNGC | 3 cut(s) | 121, 227, 851 |
| Bme1390I | CCNGG | 1 cut(s) | 19 |
| BmiI | GGNNCC | 3 cut(s) | 243, 528, 833 |
| BmrFI | CCNGG | 1 cut(s) | 19 |
| BmtI | GCTAGC | 1 cut(s) | 315 |
| BoxI | GACNNNNGTC | 2 cut(s) | 182, 393 |
| BpmI | CTGGAG | 2 cut(s) | 101, 705 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 123 |
| BpuEI | CTTGAG | 2 cut(s) | 149, 174 |
| BpuMI | CCSGG | 1 cut(s) | 19 |
| BsaHI | GRCGYC | 1 cut(s) | 832 |
| BsaJI | CCNNGG | 2 cut(s) | 18, 245 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 24, 479 |
| Bse1I | ACTGG | 4 cut(s) | 308, 816, 833, 835 |
| Bse3DI | GCAATG | 4 cut(s) | 118, 220, 322, 709 |
| BseDI | CCNNGG | 2 cut(s) | 18, 245 |
| BseGI | GGATG | 2 cut(s) | 10, 337 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 24, 479 |
| BseMI | GCAATG | 4 cut(s) | 118, 220, 322, 709 |
| BseMII | CTCAG | 3 cut(s) | 98, 137, 308 |
| BseNI | ACTGG | 4 cut(s) | 308, 816, 833, 835 |
| BseXI | GCAGC | 3 cut(s) | 106, 237, 861 |
| BsgI | GTGCAG | 3 cut(s) | 383, 485, 807 |
| BshFI | GGCC | 4 cut(s) | 17, 32, 68, 307 |
| BshNI | GGYRCC | 1 cut(s) | 831 |
| BsiSI | CCGG | 1 cut(s) | 18 |
| BslFI | GGGAC | 1 cut(s) | 263 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 24, 479 |
| BsmAI | GTCTC | 1 cut(s) | 329 |
| BsmFI | GGGAC | 1 cut(s) | 263 |
| BsnI | GGCC | 4 cut(s) | 17, 32, 68, 307 |
| Bsp1286I | GDGCHC | 1 cut(s) | 150 |
| Bsp143I | GATC | 1 cut(s) | 297 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 123 |
| BspANI | GGCC | 4 cut(s) | 17, 32, 68, 307 |
| BspCNI | CTCAG | 3 cut(s) | 99, 136, 307 |
| BspHI | TCATGA | 1 cut(s) | 475 |
| BspLI | GGNNCC | 3 cut(s) | 243, 528, 833 |
| BspMAI | CTGCAG | 1 cut(s) | 851 |
| BspMI | ACCTGC | 2 cut(s) | 764, 885 |
| BspOI | GCTAGC | 1 cut(s) | 315 |
| BspQI | GCTCTTC | 2 cut(s) | 204, 786 |
| BspT107I | GGYRCC | 1 cut(s) | 831 |
| BsrDI | GCAATG | 4 cut(s) | 118, 220, 322, 709 |
| BsrI | ACTGG | 4 cut(s) | 308, 816, 833, 835 |
| BssECI | CCNNGG | 2 cut(s) | 18, 245 |
| BssMI | GATC | 1 cut(s) | 297 |
| BssNI | GRCGYC | 1 cut(s) | 832 |
| BssT1I | CCWWGG | 1 cut(s) | 245 |
| Bst4CI | ACNGT | 1 cut(s) | 599 |
| Bst6I | CTCTTC | 2 cut(s) | 204, 786 |
| BstACI | GRCGYC | 1 cut(s) | 832 |
| BstC8I | GCNNGC | 2 cut(s) | 309, 313 |
| BstDEI | CTNAG | 3 cut(s) | 107, 123, 294 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 477 |
| BstF5I | GGATG | 2 cut(s) | 10, 337 |
| BstH2I | RGCGCY | 1 cut(s) | 835 |
| BstHHI | GCGC | 2 cut(s) | 552, 834 |
| BstKTI | GATC | 1 cut(s) | 300 |
| BstMAI | GTCTC | 1 cut(s) | 329 |
| BstMBI | GATC | 1 cut(s) | 297 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 281 |
| BstNSI | RCATGY | 1 cut(s) | 434 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 182, 393 |
| BstSCI | CCNGG | 1 cut(s) | 17 |
| BstSFI | CTRYAG | 2 cut(s) | 285, 847 |
| BstV1I | GCAGC | 3 cut(s) | 106, 237, 861 |
| BsuRI | GGCC | 4 cut(s) | 17, 32, 68, 307 |
| BtsCI | GGATG | 2 cut(s) | 10, 337 |
| BtsI | GCAGTG | 1 cut(s) | 473 |
| BtsIMutI | CAGTG | 4 cut(s) | 473, 809, 826, 842 |
| BveI | ACCTGC | 2 cut(s) | 764, 885 |
| Cac8I | GCNNGC | 2 cut(s) | 309, 313 |
| CciI | TCATGA | 1 cut(s) | 475 |
| CfoI | GCGC | 2 cut(s) | 552, 834 |
| CseI | GACGC | 2 cut(s) | 50, 143 |
| CspCI | CAANNNNNGTGG | 1 cut(s) | 872 |
| CviAII | CATG | 4 cut(s) | 151, 431, 476, 700 |
| DdeI | CTNAG | 3 cut(s) | 107, 123, 294 |
| DinI | GGCGCC | 1 cut(s) | 833 |
| DpnI | GATC | 1 cut(s) | 299 |
| DpnII | GATC | 1 cut(s) | 297 |
| EaeI | YGGCCR | 3 cut(s) | 15, 30, 305 |
| Eam1104I | CTCTTC | 2 cut(s) | 204, 786 |
| EarI | CTCTTC | 2 cut(s) | 204, 786 |
| Eco130I | CCWWGG | 1 cut(s) | 245 |
| Eco24I | GRGCYC | 1 cut(s) | 150 |
| Eco57I | CTGAAG | 1 cut(s) | 513 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 477 |
| EcoT14I | CCWWGG | 1 cut(s) | 245 |
| EcoT22I | ATGCAT | 1 cut(s) | 701 |
| EcoT38I | GRGCYC | 1 cut(s) | 150 |
| EgeI | GGCGCC | 1 cut(s) | 833 |
| EheI | GGCGCC | 1 cut(s) | 833 |
| ErhI | CCWWGG | 1 cut(s) | 245 |
| FaeI | CATG | 4 cut(s) | 154, 434, 479, 703 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 183, 215 |
| FaqI | GGGAC | 1 cut(s) | 263 |
| FatI | CATG | 4 cut(s) | 150, 430, 475, 699 |
| FauNDI | CATATG | 1 cut(s) | 855 |
| Fnu4HI | GCNGC | 3 cut(s) | 120, 226, 850 |
| FokI | GGATG | 2 cut(s) | 17, 344 |
| FriOI | GRGCYC | 1 cut(s) | 150 |
| Fsp4HI | GCNGC | 3 cut(s) | 120, 226, 850 |
| FspBI | CTAG | 4 cut(s) | 45, 246, 312, 372 |
| GlaI | GCGC | 2 cut(s) | 551, 833 |
| GluI | GCNGC | 3 cut(s) | 120, 226, 850 |
| GsuI | CTGGAG | 2 cut(s) | 101, 705 |
| HaeII | RGCGCY | 1 cut(s) | 835 |
| HaeIII | GGCC | 4 cut(s) | 17, 32, 68, 307 |
| HapII | CCGG | 1 cut(s) | 18 |
| HgaI | GACGC | 2 cut(s) | 50, 143 |
| HhaI | GCGC | 2 cut(s) | 552, 834 |
| Hin1I | GRCGYC | 1 cut(s) | 832 |
| Hin1II | CATG | 4 cut(s) | 154, 434, 479, 703 |
| Hin6I | GCGC | 2 cut(s) | 550, 832 |
| HinP1I | GCGC | 2 cut(s) | 550, 832 |
| HinfI | GANTC | 5 cut(s) | 76, 413, 488, 566, 687 |
| HpaII | CCGG | 1 cut(s) | 18 |
| HphI | GGTGA | 2 cut(s) | 110, 814 |
| Hpy166II | GTNNAC | 2 cut(s) | 595, 844 |
| Hpy188I | TCNGA | 4 cut(s) | 57, 108, 297, 493 |
| Hpy188III | TCNNGA | 6 cut(s) | 80, 158, 169, 476, 684, 691 |
| Hpy8I | GTNNAC | 2 cut(s) | 595, 844 |
| HpyAV | CCTTC | 5 cut(s) | 137, 240, 473, 583, 866 |
| HpyCH4III | ACNGT | 1 cut(s) | 599 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 281 |
| HpyF3I | CTNAG | 3 cut(s) | 107, 123, 294 |
| Hsp92I | GRCGYC | 1 cut(s) | 832 |
| Hsp92II | CATG | 4 cut(s) | 154, 434, 479, 703 |
| HspAI | GCGC | 2 cut(s) | 550, 832 |
| KasI | GGCGCC | 1 cut(s) | 831 |
| Kzo9I | GATC | 1 cut(s) | 297 |
| LguI | GCTCTTC | 2 cut(s) | 204, 786 |
| LmnI | GCTCC | 2 cut(s) | 456, 526 |
| Lsp1109I | GCAGC | 3 cut(s) | 106, 237, 861 |
| MaeI | CTAG | 4 cut(s) | 45, 246, 312, 372 |
| MaeIII | GTNAC | 5 cut(s) | 38, 184, 580, 807, 878 |
| MalI | GATC | 1 cut(s) | 299 |
| MboI | GATC | 1 cut(s) | 297 |
| MboII | GAAGA | 5 cut(s) | 85, 191, 368, 561, 803 |
| MfeI | CAATTG | 3 cut(s) | 384, 727, 797 |
| MhlI | GDGCHC | 1 cut(s) | 150 |
| MlsI | TGGCCA | 2 cut(s) | 32, 307 |
| MluCI | AATT | 6 cut(s) | 212, 269, 345, 384, 727, 797 |
| MluNI | TGGCCA | 2 cut(s) | 32, 307 |
| Mly113I | GGCGCC | 1 cut(s) | 832 |
| MlyI | GAGTC | 2 cut(s) | 497, 696 |
| MnlI | CCTC | 7 cut(s) | 58, 76, 94, 109, 124, 274, 483 |
| Mox20I | TGGCCA | 2 cut(s) | 32, 307 |
| Mph1103I | ATGCAT | 1 cut(s) | 701 |
| MroXI | GAANNNNTTC | 1 cut(s) | 379 |
| MscI | TGGCCA | 2 cut(s) | 32, 307 |
| MseI | TTAA | 3 cut(s) | 255, 450, 623 |
| Msp20I | TGGCCA | 2 cut(s) | 32, 307 |
| MspI | CCGG | 1 cut(s) | 18 |
| MspR9I | CCNGG | 1 cut(s) | 19 |
| MunI | CAATTG | 3 cut(s) | 384, 727, 797 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 281 |
| NarI | GGCGCC | 1 cut(s) | 832 |
| NciI | CCSGG | 1 cut(s) | 19 |
| NdeI | CATATG | 1 cut(s) | 855 |
| NdeII | GATC | 1 cut(s) | 297 |
| NheI | GCTAGC | 1 cut(s) | 311 |
| NlaIII | CATG | 4 cut(s) | 154, 434, 479, 703 |
| NlaIV | GGNNCC | 3 cut(s) | 243, 528, 833 |
| NmuCI | GTSAC | 3 cut(s) | 38, 184, 807 |
| NsiI | ATGCAT | 1 cut(s) | 701 |
| NspI | RCATGY | 1 cut(s) | 434 |
| PagI | TCATGA | 1 cut(s) | 475 |
| PaqCI | CACCTGC | 1 cut(s) | 885 |
| PciSI | GCTCTTC | 2 cut(s) | 204, 786 |
| PdmI | GAANNNNTTC | 1 cut(s) | 379 |
| PfeI | GAWTC | 3 cut(s) | 76, 413, 566 |
| PkrI | GCNGC | 3 cut(s) | 121, 227, 851 |
| PleI | GAGTC | 2 cut(s) | 496, 695 |
| PluTI | GGCGCC | 1 cut(s) | 835 |
| PpsI | GAGTC | 2 cut(s) | 496, 695 |
| PshAI | GACNNNNGTC | 2 cut(s) | 182, 393 |
| PspN4I | GGNNCC | 3 cut(s) | 243, 528, 833 |
| PstI | CTGCAG | 1 cut(s) | 851 |
| SapI | GCTCTTC | 2 cut(s) | 204, 786 |
| SaqAI | TTAA | 3 cut(s) | 255, 450, 623 |
| SatI | GCNGC | 3 cut(s) | 120, 226, 850 |
| Sau3AI | GATC | 1 cut(s) | 297 |
| SchI | GAGTC | 2 cut(s) | 497, 696 |
| ScrFI | CCNGG | 1 cut(s) | 19 |
| SduI | GDGCHC | 1 cut(s) | 150 |
| SfcI | CTRYAG | 2 cut(s) | 285, 847 |
| SfoI | GGCGCC | 1 cut(s) | 833 |
| SmlI | CTYRAG | 2 cut(s) | 128, 189 |
| SmoI | CTYRAG | 2 cut(s) | 128, 189 |
| Sse9I | AATT | 6 cut(s) | 212, 269, 345, 384, 727, 797 |
| SspDI | GGCGCC | 1 cut(s) | 831 |
| SspMI | CTAG | 4 cut(s) | 45, 246, 312, 372 |
| StyD4I | CCNGG | 1 cut(s) | 17 |
| StyI | CCWWGG | 1 cut(s) | 245 |
| TaaI | ACNGT | 1 cut(s) | 599 |
| TaqI | TCGA | 1 cut(s) | 744 |
| TasI | AATT | 6 cut(s) | 212, 269, 345, 384, 727, 797 |
| TfiI | GAWTC | 3 cut(s) | 76, 413, 566 |
| Tru1I | TTAA | 3 cut(s) | 255, 450, 623 |
| Tru9I | TTAA | 3 cut(s) | 255, 450, 623 |
| TscAI | CASTG | 4 cut(s) | 473, 816, 833, 842 |
| TseFI | GTSAC | 3 cut(s) | 38, 184, 807 |
| TseI | GCWGC | 3 cut(s) | 119, 225, 849 |
| Tsp45I | GTSAC | 3 cut(s) | 38, 184, 807 |
| TspDTI | ATGAA | 2 cut(s) | 437, 492 |
| TspGWI | ACGGA | 1 cut(s) | 599 |
| TspRI | CASTG | 4 cut(s) | 473, 816, 833, 842 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 477 |
| XapI | RAATTY | 1 cut(s) | 212 |
| XceI | RCATGY | 1 cut(s) | 434 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 76 |
| XmaJI | CCTAGG | 1 cut(s) | 245 |
| XmnI | GAANNNNTTC | 1 cut(s) | 379 |
| XspI | CTAG | 4 cut(s) | 45, 246, 312, 372 |
| Zsp2I | ATGCAT | 1 cut(s) | 701 |