E3 ubiquitin-protein ligase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 557 |
| AccB1I | GGYRCC | 2 cut(s) | 624, 1096 |
| AclWI | GGATC | 1 cut(s) | 392 |
| AcoI | YGGCCR | 2 cut(s) | 15, 30 |
| AcsI | RAATTY | 1 cut(s) | 857 |
| AcuI | CTGAAG | 2 cut(s) | 306, 1089 |
| AcyI | GRCGYC | 2 cut(s) | 625, 1097 |
| AdeI | CACNNNGTG | 1 cut(s) | 893 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 24, 272 |
| AhlI | ACTAGT | 1 cut(s) | 164 |
| AjnI | CCWGG | 2 cut(s) | 802, 947 |
| AluBI | AGCT | 3 cut(s) | 127, 303, 929 |
| AluI | AGCT | 3 cut(s) | 127, 303, 929 |
| Alw26I | GTCTC | 1 cut(s) | 980 |
| AlwI | GGATC | 1 cut(s) | 392 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1067 |
| AoxI | GGCC | 4 cut(s) | 15, 30, 66, 950 |
| ApeKI | GCWGC | 3 cut(s) | 119, 642, 870 |
| ApoI | RAATTY | 1 cut(s) | 857 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 887, 919 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1030 |
| AspLEI | GCGC | 3 cut(s) | 345, 627, 1099 |
| AsuC2I | CCSGG | 1 cut(s) | 19 |
| AsuHPI | GGTGA | 2 cut(s) | 110, 607 |
| AsuII | TTCGAA | 1 cut(s) | 683 |
| BalI | TGGCCA | 1 cut(s) | 32 |
| BanI | GGYRCC | 2 cut(s) | 624, 1096 |
| BanII | GRGCYC | 1 cut(s) | 150 |
| BbsI | GAAGAC | 1 cut(s) | 1063 |
| BbvI | GCAGC | 3 cut(s) | 106, 654, 882 |
| BccI | CCATC | 2 cut(s) | 767, 1034 |
| BciT130I | CCWGG | 2 cut(s) | 804, 949 |
| BcnI | CCSGG | 1 cut(s) | 19 |
| BcoDI | GTCTC | 1 cut(s) | 980 |
| BcuI | ACTAGT | 1 cut(s) | 164 |
| BfaI | CTAG | 4 cut(s) | 45, 165, 726, 963 |
| BfmI | CTRYAG | 3 cut(s) | 579, 640, 954 |
| BfoI | RGCGCY | 2 cut(s) | 628, 1100 |
| BfuAI | ACCTGC | 1 cut(s) | 557 |
| BisI | GCNGC | 3 cut(s) | 120, 643, 871 |
| BlpI | GCTNAGC | 1 cut(s) | 123 |
| BlsI | GCNGC | 3 cut(s) | 121, 644, 872 |
| Bme1390I | CCNGG | 3 cut(s) | 19, 804, 949 |
| BmiI | GGNNCC | 3 cut(s) | 321, 626, 1098 |
| BmrFI | CCNGG | 3 cut(s) | 19, 804, 949 |
| BpiI | GAAGAC | 1 cut(s) | 1063 |
| BpmI | CTGGAG | 3 cut(s) | 101, 498, 782 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 123 |
| Bpu14I | TTCGAA | 1 cut(s) | 683 |
| BpuEI | CTTGAG | 1 cut(s) | 149 |
| BpuMI | CCSGG | 1 cut(s) | 19 |
| BsaAI | YACGTR | 1 cut(s) | 890 |
| BsaHI | GRCGYC | 2 cut(s) | 625, 1097 |
| BsaJI | CCNNGG | 2 cut(s) | 18, 803 |
| BsaWI | WCCGGW | 1 cut(s) | 55 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 24, 272 |
| Bse1I | ACTGG | 6 cut(s) | 609, 626, 628, 1061, 1098, 1100 |
| Bse3DI | GCAATG | 2 cut(s) | 118, 240 |
| BseBI | CCWGG | 2 cut(s) | 804, 949 |
| BseDI | CCNNGG | 2 cut(s) | 18, 803 |
| BseGI | GGATG | 1 cut(s) | 10 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 24, 272 |
| BseMI | GCAATG | 2 cut(s) | 118, 240 |
| BseMII | CTCAG | 2 cut(s) | 98, 114 |
| BseNI | ACTGG | 6 cut(s) | 609, 626, 628, 1061, 1098, 1100 |
| BseXI | GCAGC | 3 cut(s) | 106, 654, 882 |
| BseYI | CCCAGC | 2 cut(s) | 698, 704 |
| BsgI | GTGCAG | 2 cut(s) | 176, 1034 |
| BshFI | GGCC | 4 cut(s) | 17, 32, 68, 952 |
| BshNI | GGYRCC | 2 cut(s) | 624, 1096 |
| BsiSI | CCGG | 2 cut(s) | 18, 56 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 24, 272 |
| BsmAI | GTCTC | 1 cut(s) | 980 |
| BsnI | GGCC | 4 cut(s) | 17, 32, 68, 952 |
| Bsp119I | TTCGAA | 1 cut(s) | 683 |
| Bsp1286I | GDGCHC | 1 cut(s) | 150 |
| Bsp143I | GATC | 1 cut(s) | 397 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 123 |
| BspANI | GGCC | 4 cut(s) | 17, 32, 68, 952 |
| BspCNI | CTCAG | 2 cut(s) | 99, 115 |
| BspHI | TCATGA | 1 cut(s) | 268 |
| BspLI | GGNNCC | 3 cut(s) | 321, 626, 1098 |
| BspMAI | CTGCAG | 3 cut(s) | 583, 644, 958 |
| BspMI | ACCTGC | 1 cut(s) | 557 |
| BspPI | GGATC | 1 cut(s) | 392 |
| BspQI | GCTCTTC | 1 cut(s) | 579 |
| BspT104I | TTCGAA | 1 cut(s) | 683 |
| BspT107I | GGYRCC | 2 cut(s) | 624, 1096 |
| BsrDI | GCAATG | 2 cut(s) | 118, 240 |
| BsrI | ACTGG | 6 cut(s) | 609, 626, 628, 1061, 1098, 1100 |
| BssECI | CCNNGG | 2 cut(s) | 18, 803 |
| BssMI | GATC | 1 cut(s) | 397 |
| BssNI | GRCGYC | 2 cut(s) | 625, 1097 |
| Bst2UI | CCWGG | 2 cut(s) | 804, 949 |
| Bst4CI | ACNGT | 1 cut(s) | 392 |
| Bst6I | CTCTTC | 2 cut(s) | 579, 660 |
| BstACI | GRCGYC | 2 cut(s) | 625, 1097 |
| BstBAI | YACGTR | 1 cut(s) | 890 |
| BstBI | TTCGAA | 1 cut(s) | 683 |
| BstC8I | GCNNGC | 1 cut(s) | 954 |
| BstDEI | CTNAG | 3 cut(s) | 107, 123, 447 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 270 |
| BstF5I | GGATG | 1 cut(s) | 10 |
| BstH2I | RGCGCY | 2 cut(s) | 628, 1100 |
| BstHHI | GCGC | 3 cut(s) | 345, 627, 1099 |
| BstKTI | GATC | 1 cut(s) | 400 |
| BstMAI | GTCTC | 1 cut(s) | 980 |
| BstMBI | GATC | 1 cut(s) | 397 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 710, 926 |
| BstNI | CCWGG | 2 cut(s) | 804, 949 |
| BstSCI | CCNGG | 3 cut(s) | 17, 802, 947 |
| BstSFI | CTRYAG | 3 cut(s) | 579, 640, 954 |
| BstV1I | GCAGC | 3 cut(s) | 106, 654, 882 |
| BstV2I | GAAGAC | 1 cut(s) | 1063 |
| BstX2I | RGATCY | 1 cut(s) | 397 |
| BstYI | RGATCY | 1 cut(s) | 397 |
| BsuRI | GGCC | 4 cut(s) | 17, 32, 68, 952 |
| BtgZI | GCGATG | 1 cut(s) | 488 |
| BtsCI | GGATG | 1 cut(s) | 10 |
| BtsI | GCAGTG | 1 cut(s) | 973 |
| BtsIMutI | CAGTG | 8 cut(s) | 266, 602, 619, 635, 973, 1011, 1091, 1107 |
| BveI | ACCTGC | 1 cut(s) | 557 |
| Cac8I | GCNNGC | 1 cut(s) | 954 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1067 |
| CciI | TCATGA | 1 cut(s) | 268 |
| CfoI | GCGC | 3 cut(s) | 345, 627, 1099 |
| CseI | GACGC | 1 cut(s) | 50 |
| CviAII | CATG | 4 cut(s) | 253, 269, 340, 493 |
| DdeI | CTNAG | 3 cut(s) | 107, 123, 447 |
| DinI | GGCGCC | 2 cut(s) | 626, 1098 |
| DpnI | GATC | 1 cut(s) | 399 |
| DpnII | GATC | 1 cut(s) | 397 |
| DraIII | CACNNNGTG | 1 cut(s) | 893 |
| EaeI | YGGCCR | 2 cut(s) | 15, 30 |
| Eam1104I | CTCTTC | 2 cut(s) | 579, 660 |
| EarI | CTCTTC | 2 cut(s) | 579, 660 |
| Eco24I | GRGCYC | 1 cut(s) | 150 |
| Eco57I | CTGAAG | 2 cut(s) | 306, 1089 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 270 |
| EcoRII | CCWGG | 2 cut(s) | 802, 947 |
| EcoT22I | ATGCAT | 1 cut(s) | 494 |
| EcoT38I | GRGCYC | 1 cut(s) | 150 |
| EgeI | GGCGCC | 2 cut(s) | 626, 1098 |
| EheI | GGCGCC | 2 cut(s) | 626, 1098 |
| FaeI | CATG | 4 cut(s) | 256, 272, 343, 496 |
| FatI | CATG | 4 cut(s) | 252, 268, 339, 492 |
| FauNDI | CATATG | 1 cut(s) | 648 |
| Fnu4HI | GCNGC | 3 cut(s) | 120, 643, 871 |
| FokI | GGATG | 1 cut(s) | 17 |
| FriOI | GRGCYC | 1 cut(s) | 150 |
| Fsp4HI | GCNGC | 3 cut(s) | 120, 643, 871 |
| FspBI | CTAG | 4 cut(s) | 45, 165, 726, 963 |
| GlaI | GCGC | 3 cut(s) | 344, 626, 1098 |
| GluI | GCNGC | 3 cut(s) | 120, 643, 871 |
| GsaI | CCCAGC | 2 cut(s) | 702, 708 |
| GsuI | CTGGAG | 3 cut(s) | 101, 498, 782 |
| HaeII | RGCGCY | 2 cut(s) | 628, 1100 |
| HaeIII | GGCC | 4 cut(s) | 17, 32, 68, 952 |
| HapII | CCGG | 2 cut(s) | 18, 56 |
| HgaI | GACGC | 1 cut(s) | 50 |
| HhaI | GCGC | 3 cut(s) | 345, 627, 1099 |
| Hin1I | GRCGYC | 2 cut(s) | 625, 1097 |
| Hin1II | CATG | 4 cut(s) | 256, 272, 343, 496 |
| Hin6I | GCGC | 3 cut(s) | 343, 625, 1097 |
| HinP1I | GCGC | 3 cut(s) | 343, 625, 1097 |
| HinfI | GANTC | 7 cut(s) | 76, 281, 351, 359, 480, 680, 757 |
| HpaII | CCGG | 2 cut(s) | 18, 56 |
| HphI | GGTGA | 2 cut(s) | 110, 607 |
| Hpy166II | GTNNAC | 2 cut(s) | 388, 637 |
| Hpy188I | TCNGA | 3 cut(s) | 108, 286, 738 |
| Hpy188III | TCNNGA | 5 cut(s) | 80, 269, 395, 477, 761 |
| Hpy8I | GTNNAC | 2 cut(s) | 388, 637 |
| HpyAV | CCTTC | 6 cut(s) | 137, 176, 266, 585, 885, 976 |
| HpyCH4III | ACNGT | 1 cut(s) | 392 |
| HpyCH4IV | ACGT | 1 cut(s) | 889 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 710, 926 |
| HpyF3I | CTNAG | 3 cut(s) | 107, 123, 447 |
| HpySE526I | ACGT | 1 cut(s) | 889 |
| Hsp92I | GRCGYC | 2 cut(s) | 625, 1097 |
| Hsp92II | CATG | 4 cut(s) | 256, 272, 343, 496 |
| HspAI | GCGC | 3 cut(s) | 343, 625, 1097 |
| KasI | GGCGCC | 2 cut(s) | 624, 1096 |
| Kzo9I | GATC | 1 cut(s) | 397 |
| LguI | GCTCTTC | 1 cut(s) | 579 |
| LmnI | GCTCC | 2 cut(s) | 249, 319 |
| Lsp1109I | GCAGC | 3 cut(s) | 106, 654, 882 |
| MaeI | CTAG | 4 cut(s) | 45, 165, 726, 963 |
| MaeII | ACGT | 1 cut(s) | 889 |
| MaeIII | GTNAC | 6 cut(s) | 38, 160, 600, 719, 784, 893 |
| MalI | GATC | 1 cut(s) | 399 |
| MboI | GATC | 1 cut(s) | 397 |
| MboII | GAAGA | 6 cut(s) | 85, 596, 677, 766, 864, 1068 |
| MfeI | CAATTG | 4 cut(s) | 177, 520, 590, 1035 |
| MflI | RGATCY | 1 cut(s) | 397 |
| MhlI | GDGCHC | 1 cut(s) | 150 |
| MlsI | TGGCCA | 1 cut(s) | 32 |
| MluNI | TGGCCA | 1 cut(s) | 32 |
| Mly113I | GGCGCC | 2 cut(s) | 625, 1097 |
| MlyI | GAGTC | 2 cut(s) | 290, 489 |
| Mox20I | TGGCCA | 1 cut(s) | 32 |
| Mph1103I | ATGCAT | 1 cut(s) | 494 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1030 |
| MscI | TGGCCA | 1 cut(s) | 32 |
| MseI | TTAA | 4 cut(s) | 243, 375, 900, 1108 |
| Msp20I | TGGCCA | 1 cut(s) | 32 |
| MspI | CCGG | 2 cut(s) | 18, 56 |
| MspR9I | CCNGG | 3 cut(s) | 19, 804, 949 |
| MunI | CAATTG | 4 cut(s) | 177, 520, 590, 1035 |
| MvaI | CCWGG | 2 cut(s) | 804, 949 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 710, 926 |
| NarI | GGCGCC | 2 cut(s) | 625, 1097 |
| NciI | CCSGG | 1 cut(s) | 19 |
| NdeI | CATATG | 1 cut(s) | 648 |
| NdeII | GATC | 1 cut(s) | 397 |
| NlaIII | CATG | 4 cut(s) | 256, 272, 343, 496 |
| NlaIV | GGNNCC | 3 cut(s) | 321, 626, 1098 |
| NmuCI | GTSAC | 4 cut(s) | 38, 600, 719, 893 |
| NsiI | ATGCAT | 1 cut(s) | 494 |
| NspV | TTCGAA | 1 cut(s) | 683 |
| PagI | TCATGA | 1 cut(s) | 268 |
| PciSI | GCTCTTC | 1 cut(s) | 579 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1030 |
| PfeI | GAWTC | 5 cut(s) | 76, 351, 359, 680, 757 |
| PkrI | GCNGC | 3 cut(s) | 121, 644, 872 |
| PleI | GAGTC | 2 cut(s) | 289, 488 |
| PluTI | GGCGCC | 2 cut(s) | 628, 1100 |
| PpsI | GAGTC | 2 cut(s) | 289, 488 |
| Ppu21I | YACGTR | 1 cut(s) | 890 |
| Psp6I | CCWGG | 2 cut(s) | 802, 947 |
| PspFI | CCCAGC | 2 cut(s) | 698, 704 |
| PspGI | CCWGG | 2 cut(s) | 802, 947 |
| PspN4I | GGNNCC | 3 cut(s) | 321, 626, 1098 |
| PstI | CTGCAG | 3 cut(s) | 583, 644, 958 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1067 |
| PsuI | RGATCY | 1 cut(s) | 397 |
| SapI | GCTCTTC | 1 cut(s) | 579 |
| SaqAI | TTAA | 4 cut(s) | 243, 375, 900, 1108 |
| SatI | GCNGC | 3 cut(s) | 120, 643, 871 |
| Sau3AI | GATC | 1 cut(s) | 397 |
| SchI | GAGTC | 2 cut(s) | 290, 489 |
| ScrFI | CCNGG | 3 cut(s) | 19, 804, 949 |
| SduI | GDGCHC | 1 cut(s) | 150 |
| SfcI | CTRYAG | 3 cut(s) | 579, 640, 954 |
| SfoI | GGCGCC | 2 cut(s) | 626, 1098 |
| SfuI | TTCGAA | 1 cut(s) | 683 |
| SmlI | CTYRAG | 1 cut(s) | 128 |
| SmoI | CTYRAG | 1 cut(s) | 128 |
| SpeI | ACTAGT | 1 cut(s) | 164 |
| SspDI | GGCGCC | 2 cut(s) | 624, 1096 |
| SspI | AATATT | 1 cut(s) | 866 |
| SspMI | CTAG | 4 cut(s) | 45, 165, 726, 963 |
| StyD4I | CCNGG | 3 cut(s) | 17, 802, 947 |
| TaaI | ACNGT | 1 cut(s) | 392 |
| TaiI | ACGT | 1 cut(s) | 892 |
| TaqI | TCGA | 3 cut(s) | 683, 744, 1024 |
| TfiI | GAWTC | 5 cut(s) | 76, 351, 359, 680, 757 |
| Tru1I | TTAA | 4 cut(s) | 243, 375, 900, 1108 |
| Tru9I | TTAA | 4 cut(s) | 243, 375, 900, 1108 |
| TscAI | CASTG | 8 cut(s) | 266, 609, 626, 635, 973, 1018, 1098, 1107 |
| TseFI | GTSAC | 4 cut(s) | 38, 600, 719, 893 |
| TseI | GCWGC | 3 cut(s) | 119, 642, 870 |
| Tsp45I | GTSAC | 4 cut(s) | 38, 600, 719, 893 |
| TspDTI | ATGAA | 3 cut(s) | 230, 285, 1101 |
| TspRI | CASTG | 8 cut(s) | 266, 609, 626, 635, 973, 1018, 1098, 1107 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 270 |
| XapI | RAATTY | 1 cut(s) | 857 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 76 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1030 |
| XspI | CTAG | 4 cut(s) | 45, 165, 726, 963 |
| Zsp2I | ATGCAT | 1 cut(s) | 494 |