FvH4_4g08950

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000533
Physical Location & Seq
Reverse (-)
9958143 .. 9960069
1927 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g08950.t1

Sequence Viewer

Length: 1485 bp
ATGGAGCAATCGACTAGAGCCGCTAGACTTCTGCTCTTGAATCTTTTTATGCTTGGCGTTCATGCTCACTCAGCAGTTCATTCATTCGCCAATGCATCTGCATCTGATCATGTTGGTGGTGGAACAGCAGGTTGTCCATTAGCAGCAACATTATCAGCAAACTACTCGGTGATTGTCCTTGAAAGGGGTGGTGCTCCTACTGCACATCCAAGTGTCTTGCAAGAAGAAGGGTTTCTGAGTACTCTCATGCAGGAAGATGATGGAAAGACCACACCAGCTGAGAGGTTTACATCAGAAGATGGTGTTGCTATTGTTAGAGGAAGGGTTTTAGGTGGATCGAGCATGATCAATGGCGGTTTCTTCTCCAAAGCAGACGAAGAATTCTTTGATAAATACGGAATTGAATGGGACATGGATTTGGTTGAAAAGGCGTATCACTGGGTTGAAAACACTGTTGTCTTTCATCCAAATTTTTCATACACAAATTGGCAATCTGTTGTGAAAGAAGCATTGTTGGAGGCTGGTGTTGGTCCGAACAACGGATTCAGTTTGGATCACATCAAGGGAACTAAAGTTGGTGGTTCTACTTTTGATATACATGGAAGGAGACACGGAGCTGTGGAGTTGCTTAACCAAGGAAACCTAAAGAACTTGGAGTGGGAATTCATGCCACAGTCGAGAAGATCATCTTCTCTTCCAAGAGATCAAGGAGAGGTTATTCTGAGTGCAGGGGCAATTGGGAGTCCTCAGCTTCTACTTCTTAGCGGTGTTGGCCCTCAGTCCTACCTATCCTCTTTGAAAATCCCAGTTGTTCACCCCCAACCTCACACTGGACAATTCATGTATGACAATCCTCGTAATTCCATTAGCATTTTATCCCCGTTTCCACTAGAGCCCTCATTGATAAAGATTGCTGGTATTACCGACAATTACTACATAGAGGCCTTCAGTGTTGGCACTCCAATTTCTACAACACCCTTCAGTATTTTTCCGGATCCAACTAAACATATTGAGATAAATTCAAGTTGGGGACAGATTTGTATGAAAATCGAGGGACCATCTTATGGTAATCTTAAGCTTCAATCATCATCTGATGTGAGAGTCGGTCCAAATGTCCGGTTCAACTACTTTGCAGATCCATTGGACCTTGCTCATTGTGTTTCTGGCATGAAAAAGATTGGAGAGTTGTTAAACACCAAGGCACTGCAAGCATTTAAGGTTGAAGCTTCGTCGAGCACAAAAGCTTACAAGTTTTATGGTCCCACTTTACCCATGAACCAGACAAACGATGCATCTTTCGGAGAATTTTGCCGTGATACAGTATCAACAATTTGGCATTACCACGGCGGATGCCTCGTCGGAAAGGTGGTTGATAGCAGCCTGCGGGTCATGGGAATCGATACGTCACGTGTTGTTGACGCATCCACATTCAATTTCACACCGGGGACAAATCCTCAGGCAACCCTTATGATGCTGGGCCGCTGA

Protein Analysis

495

Amino Acids

53.62

Weight (kDa)

5.97

Isoelectric Point (pI)

37.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_C PF05199 354 - 493 6.8e-24 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 119
AccB7I CCANNNNNTGG 1 cut(s) 1064
AccIII TCCGGA 1 cut(s) 991
AciI CCGC 6 cut(s) 21, 354, 765, 1347, 1384, 1480
AclWI GGATC 5 cut(s) 343, 561, 989, 1002, 1130
AcsI RAATTY 5 cut(s) 380, 469, 662, 1018, 1304
AcuI CTGAAG 2 cut(s) 931, 964
AcvI CACGTG 1 cut(s) 1409
AfaI GTAC 1 cut(s) 241
AfiI CCNNNNNNNGG 4 cut(s) 184, 539, 830, 1064
AflII CTTAAG 1 cut(s) 1073
AflIII ACRYGT 1 cut(s) 1408
AluBI AGCT 6 cut(s) 278, 617, 751, 1078, 1226, 1244
AluI AGCT 6 cut(s) 278, 617, 751, 1078, 1226, 1244
Alw21I GWGCWC 2 cut(s) 196, 1238
Alw26I GTCTC 1 cut(s) 601
AlwI GGATC 5 cut(s) 343, 561, 989, 1002, 1130
Aor13HI TCCGGA 1 cut(s) 991
AoxI GGCC 3 cut(s) 772, 942, 1477
ApeKI GCWGC 2 cut(s) 143, 1377
ApoI RAATTY 5 cut(s) 380, 469, 662, 1018, 1304
Asp700I GAANNNNTTC 1 cut(s) 231
AspS9I GGNCC 7 cut(s) 530, 773, 1055, 1106, 1144, 1259, 1477
AsuC2I CCSGG 1 cut(s) 1443
AsuHPI GGTGA 2 cut(s) 181, 806
AvaII GGWCC 5 cut(s) 530, 1055, 1106, 1144, 1259
AxyI CCTNAGG 1 cut(s) 1455
BamHI GGATCC 1 cut(s) 994
BanII GRGCYC 1 cut(s) 897
BbrPI CACGTG 1 cut(s) 1409
Bbv12I GWGCWC 2 cut(s) 196, 1238
BbvCI CCTCAGC 1 cut(s) 747
BbvI GCAGC 2 cut(s) 155, 1389
BccI CCATC 3 cut(s) 254, 293, 1066
BceAI ACGGC 2 cut(s) 1296, 1360
BcgI CGANNNNNNTGC 2 cut(s) 147, 181
BclI TGATCA 2 cut(s) 106, 345
BcnI CCSGG 1 cut(s) 1443
BcoDI GTCTC 1 cut(s) 601
BfaI CTAG 3 cut(s) 15, 24, 890
BfrI CTTAAG 1 cut(s) 1073
BfuAI ACCTGC 1 cut(s) 119
BisI GCNGC 4 cut(s) 21, 144, 1378, 1480
BlsI GCNGC 4 cut(s) 22, 145, 1379, 1481
BmcAI AGTACT 1 cut(s) 241
Bme1390I CCNGG 1 cut(s) 1443
Bme18I GGWCC 5 cut(s) 530, 1055, 1106, 1144, 1259
BmgT120I GGNCC 7 cut(s) 530, 773, 1055, 1106, 1144, 1259, 1477
BmiI GGNNCC 3 cut(s) 996, 1056, 1261
BmrFI CCNGG 1 cut(s) 1443
BmrI ACTGGG 2 cut(s) 448, 800
BmsI GCATC 7 cut(s) 104, 110, 1279, 1301, 1340, 1430, 1461
BmuI ACTGGG 2 cut(s) 448, 800
Bpu10I CCTNAGC 1 cut(s) 747
BpuMI CCSGG 1 cut(s) 1443
Bsa29I ATCGAT 1 cut(s) 1398
BsaAI YACGTR 1 cut(s) 1409
BsaJI CCNNGG 4 cut(s) 634, 1197, 1342, 1442
BsaWI WCCGGW 2 cut(s) 991, 1116
Bsc4I CCNNNNNNNGG 4 cut(s) 184, 539, 830, 1064
Bse1I ACTGG 3 cut(s) 443, 806, 835
Bse21I CCTNAGG 1 cut(s) 1455
BseAI TCCGGA 1 cut(s) 991
BseCI ATCGAT 1 cut(s) 1398
BseDI CCNNGG 4 cut(s) 634, 1197, 1342, 1442
BseGI GGATG 4 cut(s) 205, 463, 1355, 1421
BseLI CCNNNNNNNGG 4 cut(s) 184, 539, 830, 1064
BseMII CTCAG 7 cut(s) 84, 227, 270, 713, 761, 791, 1469
BseNI ACTGG 3 cut(s) 443, 806, 835
BseXI GCAGC 2 cut(s) 155, 1389
BseYI CCCAGC 1 cut(s) 1474
BsgI GTGCAG 2 cut(s) 186, 747
BshFI GGCC 3 cut(s) 774, 944, 1479
BshVI ATCGAT 1 cut(s) 1398
BsiHKAI GWGCWC 2 cut(s) 196, 1238
BsiSI CCGG 3 cut(s) 992, 1117, 1442
BslFI GGGAC 5 cut(s) 422, 1044, 1068, 1245, 1459
BslI CCNNNNNNNGG 4 cut(s) 184, 539, 830, 1064
BsmAI GTCTC 1 cut(s) 601
BsmFI GGGAC 5 cut(s) 422, 1044, 1068, 1245, 1459
BsnI GGCC 3 cut(s) 774, 944, 1479
Bsp1286I GDGCHC 3 cut(s) 196, 897, 1238
Bsp13I TCCGGA 1 cut(s) 991
Bsp143I GATC 8 cut(s) 106, 335, 345, 553, 683, 703, 994, 1135
BspACI CCGC 6 cut(s) 21, 354, 765, 1347, 1384, 1480
BspANI GGCC 3 cut(s) 774, 944, 1479
BspCNI CTCAG 7 cut(s) 83, 228, 271, 714, 760, 790, 1468
BspDI ATCGAT 1 cut(s) 1398
BspEI TCCGGA 1 cut(s) 991
BspLI GGNNCC 3 cut(s) 996, 1056, 1261
BspMI ACCTGC 1 cut(s) 119
BspPI GGATC 5 cut(s) 343, 561, 989, 1002, 1130
BspTI CTTAAG 1 cut(s) 1073
BsrI ACTGG 3 cut(s) 443, 806, 835
BssECI CCNNGG 4 cut(s) 634, 1197, 1342, 1442
BssMI GATC 8 cut(s) 106, 335, 345, 553, 683, 703, 994, 1135
BssT1I CCWWGG 2 cut(s) 634, 1197
Bst4CI ACNGT 3 cut(s) 454, 675, 1321
Bst6I CTCTTC 1 cut(s) 699
BstAFI CTTAAG 1 cut(s) 1073
BstBAI YACGTR 1 cut(s) 1409
BstC8I GCNNGC 2 cut(s) 1209, 1382
BstDEI CTNAG 8 cut(s) 70, 236, 279, 722, 747, 761, 777, 1455
BstDSI CCRYGG 1 cut(s) 1342
BstF5I GGATG 4 cut(s) 205, 463, 1355, 1421
BstKTI GATC 8 cut(s) 109, 338, 348, 556, 686, 706, 997, 1138
BstMAI GTCTC 1 cut(s) 601
BstMBI GATC 8 cut(s) 106, 335, 345, 553, 683, 703, 994, 1135
BstMWI GCNNNNNNNGC 4 cut(s) 71, 200, 771, 1208
BstSCI CCNGG 1 cut(s) 1441
BstV1I GCAGC 2 cut(s) 155, 1389
BstX2I RGATCY 2 cut(s) 994, 1135
BstYI RGATCY 2 cut(s) 994, 1135
Bsu15I ATCGAT 1 cut(s) 1398
Bsu36I CCTNAGG 1 cut(s) 1455
BsuRI GGCC 3 cut(s) 774, 944, 1479
BsuTUI ATCGAT 1 cut(s) 1398
BtgI CCRYGG 1 cut(s) 1342
BtsCI GGATG 4 cut(s) 205, 463, 1355, 1421
BtsI GCAGTG 1 cut(s) 1202
BtsIMutI CAGTG 5 cut(s) 436, 450, 828, 955, 1202
BveI ACCTGC 1 cut(s) 119
Cac8I GCNNGC 2 cut(s) 1209, 1382
Cfr13I GGNCC 7 cut(s) 530, 773, 1055, 1106, 1144, 1259, 1477
ClaI ATCGAT 1 cut(s) 1398
CseI GACGC 1 cut(s) 1427
Csp6I GTAC 1 cut(s) 240
CviQI GTAC 1 cut(s) 240
DdeI CTNAG 8 cut(s) 70, 236, 279, 722, 747, 761, 777, 1455
DpnI GATC 8 cut(s) 108, 337, 347, 555, 685, 705, 996, 1137
DpnII GATC 8 cut(s) 106, 335, 345, 553, 683, 703, 994, 1135
Eam1104I CTCTTC 1 cut(s) 699
EarI CTCTTC 1 cut(s) 699
EciI GGCGGA 1 cut(s) 1362
Eco130I CCWWGG 2 cut(s) 634, 1197
Eco147I AGGCCT 1 cut(s) 944
Eco24I GRGCYC 1 cut(s) 897
Eco47I GGWCC 5 cut(s) 530, 1055, 1106, 1144, 1259
Eco57I CTGAAG 2 cut(s) 931, 964
Eco72I CACGTG 1 cut(s) 1409
Eco81I CCTNAGG 1 cut(s) 1455
EcoRI GAATTC 2 cut(s) 380, 662
EcoT14I CCWWGG 2 cut(s) 634, 1197
EcoT22I ATGCAT 2 cut(s) 97, 1294
EcoT38I GRGCYC 1 cut(s) 897
ErhI CCWWGG 2 cut(s) 634, 1197
FalI AAGNNNNNCTT 2 cut(s) 673, 705
FaqI GGGAC 5 cut(s) 422, 1044, 1068, 1245, 1459
FauI CCCGC 1 cut(s) 1377
FbaI TGATCA 2 cut(s) 106, 345
Fnu4HI GCNGC 4 cut(s) 21, 144, 1378, 1480
FokI GGATG 4 cut(s) 192, 450, 1362, 1408
FriOI GRGCYC 1 cut(s) 897
Fsp4HI GCNGC 4 cut(s) 21, 144, 1378, 1480
FspBI CTAG 3 cut(s) 15, 24, 890
GluI GCNGC 4 cut(s) 21, 144, 1378, 1480
GsaI CCCAGC 1 cut(s) 1478
HaeIII GGCC 3 cut(s) 774, 944, 1479
HapII CCGG 3 cut(s) 992, 1117, 1442
HgaI GACGC 1 cut(s) 1427
HincII GTYRAC 1 cut(s) 1417
HindII GTYRAC 1 cut(s) 1417
HindIII AAGCTT 3 cut(s) 1076, 1224, 1242
HinfI GANTC 5 cut(s) 40, 543, 742, 1101, 1395
HpaII CCGG 3 cut(s) 992, 1117, 1442
HphI GGTGA 2 cut(s) 181, 806
Hpy166II GTNNAC 3 cut(s) 288, 814, 1417
Hpy188I TCNGA 8 cut(s) 106, 237, 295, 534, 723, 1093, 1301, 1361
Hpy188III TCNNGA 3 cut(s) 37, 678, 992
Hpy8I GTNNAC 3 cut(s) 288, 814, 1417
Hpy99I CGWCG 2 cut(s) 1234, 1361
HpyAV CCTTC 5 cut(s) 221, 315, 597, 955, 988
HpyCH4III ACNGT 3 cut(s) 454, 675, 1321
HpyCH4IV ACGT 2 cut(s) 1403, 1408
HpyCH4V TGCA 9 cut(s) 95, 101, 203, 220, 250, 728, 1133, 1207, 1292
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 200, 771, 1208
HpyF3I CTNAG 8 cut(s) 70, 236, 279, 722, 747, 761, 777, 1455
HpySE526I ACGT 2 cut(s) 1403, 1408
Kpn2I TCCGGA 1 cut(s) 991
Ksp22I TGATCA 2 cut(s) 106, 345
Kzo9I GATC 8 cut(s) 106, 335, 345, 553, 683, 703, 994, 1135
LmnI GCTCC 3 cut(s) 4, 199, 614
Lsp1109I GCAGC 2 cut(s) 155, 1389
LweI GCATC 7 cut(s) 104, 110, 1279, 1301, 1340, 1430, 1461
MaeI CTAG 3 cut(s) 15, 24, 890
MaeII ACGT 2 cut(s) 1403, 1408
MaeIII GTNAC 1 cut(s) 1404
MalI GATC 8 cut(s) 108, 337, 347, 555, 685, 705, 996, 1137
MboI GATC 8 cut(s) 106, 335, 345, 553, 683, 703, 994, 1135
MboII GAAGA 8 cut(s) 236, 266, 308, 352, 389, 681, 686, 693
MfeI CAATTG 1 cut(s) 735
MflI RGATCY 2 cut(s) 994, 1135
MhlI GDGCHC 3 cut(s) 196, 897, 1238
MlyI GAGTC 2 cut(s) 751, 1110
MmeI TCCRAC 3 cut(s) 495, 1022, 1339
Mph1103I ATGCAT 2 cut(s) 97, 1294
MroI TCCGGA 1 cut(s) 991
MroXI GAANNNNTTC 1 cut(s) 231
MseI TTAA 4 cut(s) 630, 1074, 1190, 1215
MslI CAYNNNNRTG 2 cut(s) 114, 210
MspA1I CMGCKG 2 cut(s) 278, 1482
MspCI CTTAAG 1 cut(s) 1073
MspI CCGG 3 cut(s) 992, 1117, 1442
MspR9I CCNGG 1 cut(s) 1443
MunI CAATTG 1 cut(s) 735
MwoI GCNNNNNNNGC 4 cut(s) 71, 200, 771, 1208
NciI CCSGG 1 cut(s) 1443
NdeII GATC 8 cut(s) 106, 335, 345, 553, 683, 703, 994, 1135
NlaIV GGNNCC 3 cut(s) 996, 1056, 1261
NmuCI GTSAC 1 cut(s) 1404
NsiI ATGCAT 2 cut(s) 97, 1294
PceI AGGCCT 1 cut(s) 944
PdmI GAANNNNTTC 1 cut(s) 231
PfeI GAWTC 3 cut(s) 40, 543, 1395
PflMI CCANNNNNTGG 1 cut(s) 1064
PkrI GCNGC 4 cut(s) 22, 145, 1379, 1481
PleI GAGTC 2 cut(s) 750, 1109
PmaCI CACGTG 1 cut(s) 1409
PmlI CACGTG 1 cut(s) 1409
PpsI GAGTC 2 cut(s) 750, 1109
Ppu21I YACGTR 1 cut(s) 1409
PspCI CACGTG 1 cut(s) 1409
PspFI CCCAGC 1 cut(s) 1474
PspN4I GGNNCC 3 cut(s) 996, 1056, 1261
PspPI GGNCC 7 cut(s) 530, 773, 1055, 1106, 1144, 1259, 1477
PsuI RGATCY 2 cut(s) 994, 1135
PvuII CAGCTG 1 cut(s) 278
RsaI GTAC 1 cut(s) 241
RsaNI GTAC 1 cut(s) 240
RseI CAYNNNNRTG 2 cut(s) 114, 210
SaqAI TTAA 4 cut(s) 630, 1074, 1190, 1215
SatI GCNGC 4 cut(s) 21, 144, 1378, 1480
Sau3AI GATC 8 cut(s) 106, 335, 345, 553, 683, 703, 994, 1135
Sau96I GGNCC 7 cut(s) 530, 773, 1055, 1106, 1144, 1259, 1477
ScaI AGTACT 1 cut(s) 241
SchI GAGTC 2 cut(s) 751, 1110
ScrFI CCNGG 1 cut(s) 1443
SduI GDGCHC 3 cut(s) 196, 897, 1238
SfaNI GCATC 7 cut(s) 104, 110, 1279, 1301, 1340, 1430, 1461
SinI GGWCC 5 cut(s) 530, 1055, 1106, 1144, 1259
SmiMI CAYNNNNRTG 2 cut(s) 114, 210
SmlI CTYRAG 1 cut(s) 1073
SmoI CTYRAG 1 cut(s) 1073
SseBI AGGCCT 1 cut(s) 944
SsiI CCGC 6 cut(s) 21, 354, 765, 1347, 1384, 1480
SspMI CTAG 3 cut(s) 15, 24, 890
StuI AGGCCT 1 cut(s) 944
StyD4I CCNGG 1 cut(s) 1441
StyI CCWWGG 2 cut(s) 634, 1197
TaaI ACNGT 3 cut(s) 454, 675, 1321
TaiI ACGT 2 cut(s) 1406, 1411
TaqI TCGA 6 cut(s) 11, 338, 677, 1050, 1232, 1398
TaqII GACCGA 1 cut(s) 1094
TatI WGTACW 1 cut(s) 239
TauI GCSGC 2 cut(s) 23, 1482
TfiI GAWTC 3 cut(s) 40, 543, 1395
Tru1I TTAA 4 cut(s) 630, 1074, 1190, 1215
Tru9I TTAA 4 cut(s) 630, 1074, 1190, 1215
TscAI CASTG 5 cut(s) 443, 457, 835, 955, 1209
TseFI GTSAC 1 cut(s) 1404
TseI GCWGC 2 cut(s) 143, 1377
Tsp45I GTSAC 1 cut(s) 1404
TspGWI ACGGA 3 cut(s) 411, 555, 627
TspRI CASTG 5 cut(s) 443, 457, 835, 955, 1209
Van91I CCANNNNNTGG 1 cut(s) 1064
Vha464I CTTAAG 1 cut(s) 1073
VpaK11BI GGWCC 5 cut(s) 530, 1055, 1106, 1144, 1259
XapI RAATTY 5 cut(s) 380, 469, 662, 1018, 1304
XcmI CCANNNNNNNNNTGG 1 cut(s) 827
XmnI GAANNNNTTC 1 cut(s) 231
XspI CTAG 3 cut(s) 15, 24, 890
ZrmI AGTACT 1 cut(s) 241
Zsp2I ATGCAT 2 cut(s) 97, 1294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.