MD00G1013000.v1.1

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000533
Physical Location & Seq
Reverse (-)
1626538 .. 1631166
4629 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1013000.v1.1.491

Sequence Viewer

Length: 1650 bp
ATGGAGAAATCAACAACTGCTGCTATATTATTGGTCTTTTACATTTTTGTCTTGTGTCCTCAACCAGGGATTCATTCACTTGCTCCTCCATCTGACGTTCATTTTAGCTACTTGAAATTTGTACGTAACGCAACTGATCTACCATTACAAGAAGAATATGACTACATTGTAGTTGGAGGAGGCACGGCAGGGTGCCCATTGGCAACAACTTTATCTGCAAACTACTCGGTGCTCCTTCTAGAAAGGGGCGATATTCCAACAACATATCCAAGTGTGTCGACTGTCGAAGGGATTCTTGAAAATTTCATGCTAGAAGATGACGGTACGACGCCGCTCCAGAGGTTCGTATCAGAAGATGGTGTAGCTAATGTAAGAGGACGTATCCTAGGCGGGACAAGTATGGTCAGTGGTGGTGCATACTCAAGAGCCGACAGTGAATTCTACAAGAAATCAGGAATTAAATTGGACATGAACTTAGTCAATAAGTCATATGAATGGGTTGAAGACACTATCGTATTCCGTCCGAATGTATCACAGTGGCAATCTGTTGTGAAAGATGCATTGTTAGAAGCTGGTGTTCGTCCAGACAATGGATTCACTTTGGATAGCGTTGAGGGAACTAAAATCTCGGGTGCGTTGTTTGATAATCGTGGAAGAAGATATGGAGCTGTGGAACTGCTAAATAAAGGACATCCGAAAAACCTGAGAGTTGCAATTCATGCCACTGTAGAGAGGATCATTTTCTCTTCCAAAGCATCAGATCCGTCAGCTAAAGGAATCATATACAATGACTCAAACGGGAGGTCTCACTGGGCATCGATACGTGGTAAGGGTGAGGTTATATTGAGTGCAGGGGCAATTGGGAGTCCTCAGCTTCTACTACTTAGTGGTGTTGGCCCAAAGTCCTACCTTACATCTCTTAAAATCCCAGTTGTTCACCCACAACCTTACGTTGGAAAGTTTATGCGTGACAATCCTCGTAGTAACATTATCATTTTGCCCCCATCTCCAATAGTACCTACTTATTCTCAGATTGCTGGTTTCACAAGTGATTTCGATATAGAGTCTATCTCTGGCACGCCATATTCTAGTCAGGCCTATAGTATTTTCCCTAATCCAACTATTCCCGTGACGATAAATTCAAGTTTCGGGTTCTTTATGGTCAAAGTTAGAGGACCCATATTGTCCCATGGTTCTCTTAAACTGCAATCATCGTATGATGCCAAAGTAGCCCCAAATGTCAAATTCAACTACTTTGCAAAAGAGGGGGACCTTTCTCAATGTGTTAGTGCCATGGGGAAGATGCGTGATCTATTAAAAACAAACGCATTGAAACCGTTTAAGACTCGAGATTTGCCAGGTCTAGAAGGTTTTAACCTTTTTAAACCATCTTTACCAATGAACCAGTCGGATGATGCATCTTTTTGTCGAGATACGGTAGCCACACATTGGCATTACCATGGTGGATGCTCCGCCGGAAAGGTAGTTGATGGAGATCTCCGGGTCACGGGGATCAAGGCATTACGTGTTGTTGATGGATCTATATTCAATTCATCACCAGGGACCAATCCTCAGGCCACTCTTATGATGTTAGGCAGGTATGTTGGCCTTCGAATACTGGAAGAAAGATCAGCTAGCAAGGGGCGTTGA

Protein Analysis

550

Amino Acids

59.64

Weight (kDa)

8.82

Isoelectric Point (pI)

35.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_N PF00732 53 - 322 5.7e-19 GMC oxidoreductase
GMC_oxred_C PF05199 396 - 532 1.6e-25 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1587
AccB1I GGYRCC 1 cut(s) 192
AccB7I CCANNNNNTGG 2 cut(s) 590, 1449
AccBSI CCGCTC 1 cut(s) 334
AccI GTMKAC 1 cut(s) 278
AciI CCGC 3 cut(s) 332, 390, 1473
AclWI GGATC 4 cut(s) 743, 755, 1520, 1546
AcsI RAATTY 5 cut(s) 116, 301, 437, 1138, 1244
AcyI GRCGYC 1 cut(s) 329
AfaI GTAC 3 cut(s) 123, 325, 1017
AfiI CCNNNNNNNGG 5 cut(s) 65, 590, 953, 1449, 1507
AflIII ACRYGT 1 cut(s) 1525
AgsI TTSAA 7 cut(s) 115, 299, 503, 1143, 1249, 1333, 1549
AjnI CCWGG 3 cut(s) 64, 1357, 1558
AluBI AGCT 7 cut(s) 108, 365, 572, 668, 770, 874, 1634
AluI AGCT 7 cut(s) 108, 365, 572, 668, 770, 874, 1634
Alw21I GWGCWC 1 cut(s) 234
Alw26I GTCTC 1 cut(s) 810
AlwI GGATC 4 cut(s) 743, 755, 1520, 1546
Ama87I CYCGRG 2 cut(s) 628, 1347
AoxI GGCC 4 cut(s) 895, 1095, 1575, 1606
ApeKI GCWGC 1 cut(s) 20
ApoI RAATTY 5 cut(s) 116, 301, 437, 1138, 1244
ArsI GACNNNNNNTTYG 4 cut(s) 788, 820, 1336, 1368
Asp700I GAANNNNTTC 1 cut(s) 291
AspA2I CCTAGG 1 cut(s) 385
AspS9I GGNCC 4 cut(s) 896, 1175, 1270, 1563
AsuC2I CCSGG 1 cut(s) 1502
AsuHPI GGTGA 3 cut(s) 845, 929, 1548
AsuII TTCGAA 1 cut(s) 1612
AsuNHI GCTAGC 1 cut(s) 1634
AvaI CYCGRG 2 cut(s) 628, 1347
AvaII GGWCC 3 cut(s) 1175, 1270, 1563
AvrII CCTAGG 1 cut(s) 385
AxyI CCTNAGG 1 cut(s) 1572
BaeGI GKGCMC 1 cut(s) 197
BanI GGYRCC 1 cut(s) 192
BbsI GAAGAC 1 cut(s) 510
Bbv12I GWGCWC 1 cut(s) 234
BbvCI CCTCAGC 1 cut(s) 870
BbvI GCAGC 1 cut(s) 7
BccI CCATC 6 cut(s) 97, 350, 1012, 1396, 1484, 1529
BceAI ACGGC 1 cut(s) 201
BcgI CGANNNNNNTGC 2 cut(s) 207, 241
BciT130I CCWGG 3 cut(s) 66, 1359, 1560
BciVI GTATCC 1 cut(s) 392
BcnI CCSGG 1 cut(s) 1502
BcoDI GTCTC 1 cut(s) 810
BfaI CTAG 6 cut(s) 239, 311, 386, 1089, 1364, 1635
BfmI CTRYAG 2 cut(s) 726, 1099
BfuAI ACCTGC 1 cut(s) 1587
BfuI GTATCC 1 cut(s) 392
BglII AGATCT 1 cut(s) 1495
BisI GCNGC 2 cut(s) 21, 332
BlnI CCTAGG 1 cut(s) 385
BlsI GCNGC 2 cut(s) 22, 333
Bme1390I CCNGG 4 cut(s) 66, 1359, 1502, 1560
Bme18I GGWCC 3 cut(s) 1175, 1270, 1563
BmeT110I CYCGRG 2 cut(s) 628, 1347
BmgT120I GGNCC 4 cut(s) 896, 1175, 1270, 1563
BmiI GGNNCC 4 cut(s) 194, 1177, 1271, 1564
BmrFI CCNGG 4 cut(s) 66, 1359, 1502, 1560
BmrI ACTGGG 2 cut(s) 820, 923
BmsI GCATC 8 cut(s) 547, 764, 824, 1210, 1293, 1405, 1427, 1457
BmtI GCTAGC 1 cut(s) 1638
BmuI ACTGGG 2 cut(s) 820, 923
BpiI GAAGAC 1 cut(s) 510
BplI GAGNNNNNCTC 2 cut(s) 1055, 1087
BpmI CTGGAG 1 cut(s) 320
Bpu10I CCTNAGC 1 cut(s) 870
Bpu14I TTCGAA 1 cut(s) 1612
BpuEI CTTGAG 1 cut(s) 406
BpuMI CCSGG 1 cut(s) 1502
Bsa29I ATCGAT 1 cut(s) 818
BsaAI YACGTR 3 cut(s) 125, 824, 1526
BsaBI GATNNNNATC 1 cut(s) 1494
BsaHI GRCGYC 1 cut(s) 329
BsaI GGTCTC 1 cut(s) 810
BsaJI CCNNGG 6 cut(s) 65, 385, 1189, 1293, 1459, 1559
Bsc4I CCNNNNNNNGG 5 cut(s) 65, 590, 953, 1449, 1507
Bse1I ACTGG 4 cut(s) 815, 929, 1405, 1623
Bse21I CCTNAGG 1 cut(s) 1572
Bse8I GATNNNNATC 1 cut(s) 1494
BseBI CCWGG 3 cut(s) 66, 1359, 1560
BseCI ATCGAT 1 cut(s) 818
BseDI CCNNGG 6 cut(s) 65, 385, 1189, 1293, 1459, 1559
BseGI GGATG 3 cut(s) 691, 1417, 1472
BseJI GATNNNNATC 1 cut(s) 1494
BseLI CCNNNNNNNGG 5 cut(s) 65, 590, 953, 1449, 1507
BseMII CTCAG 4 cut(s) 695, 884, 1043, 1586
BseNI ACTGG 4 cut(s) 815, 929, 1405, 1623
BseRI GAGGAG 2 cut(s) 75, 192
BseSI GKGCMC 1 cut(s) 197
BseXI GCAGC 1 cut(s) 7
BsgI GTGCAG 1 cut(s) 870
BshFI GGCC 4 cut(s) 897, 1097, 1577, 1608
BshNI GGYRCC 1 cut(s) 192
BshVI ATCGAT 1 cut(s) 818
BsiHKAI GWGCWC 1 cut(s) 234
BsiHKCI CYCGRG 2 cut(s) 628, 1347
BsiSI CCGG 2 cut(s) 1476, 1501
BslFI GGGAC 4 cut(s) 406, 1171, 1283, 1576
BslI CCNNNNNNNGG 5 cut(s) 65, 590, 953, 1449, 1507
BsmAI GTCTC 1 cut(s) 810
BsmFI GGGAC 4 cut(s) 406, 1171, 1283, 1576
BsnI GGCC 4 cut(s) 897, 1097, 1577, 1608
Bso31I GGTCTC 1 cut(s) 810
BsoBI CYCGRG 2 cut(s) 628, 1347
Bsp119I TTCGAA 1 cut(s) 1612
Bsp1286I GDGCHC 2 cut(s) 197, 234
Bsp143I GATC 8 cut(s) 136, 735, 760, 1309, 1495, 1512, 1538, 1628
Bsp19I CCATGG 3 cut(s) 1189, 1293, 1459
BspACI CCGC 3 cut(s) 332, 390, 1473
BspANI GGCC 4 cut(s) 897, 1097, 1577, 1608
BspCNI CTCAG 4 cut(s) 696, 883, 1042, 1585
BspDI ATCGAT 1 cut(s) 818
BspLI GGNNCC 4 cut(s) 194, 1177, 1271, 1564
BspMI ACCTGC 1 cut(s) 1587
BspOI GCTAGC 1 cut(s) 1638
BspPI GGATC 4 cut(s) 743, 755, 1520, 1546
BspT104I TTCGAA 1 cut(s) 1612
BspT107I GGYRCC 1 cut(s) 192
BspTNI GGTCTC 1 cut(s) 810
BsrBI CCGCTC 1 cut(s) 334
BsrI ACTGG 4 cut(s) 815, 929, 1405, 1623
BssECI CCNNGG 6 cut(s) 65, 385, 1189, 1293, 1459, 1559
BssMI GATC 8 cut(s) 136, 735, 760, 1309, 1495, 1512, 1538, 1628
BssNI GRCGYC 1 cut(s) 329
BssT1I CCWWGG 4 cut(s) 385, 1189, 1293, 1459
Bst2UI CCWGG 3 cut(s) 66, 1359, 1560
Bst4CI ACNGT 7 cut(s) 283, 323, 434, 537, 727, 1338, 1438
Bst6I CTCTTC 1 cut(s) 751
BstACI GRCGYC 1 cut(s) 329
BstAPI GCANNNNNTGC 1 cut(s) 719
BstBAI YACGTR 3 cut(s) 125, 824, 1526
BstBI TTCGAA 1 cut(s) 1612
BstC8I GCNNGC 2 cut(s) 1079, 1636
BstDEI CTNAG 6 cut(s) 475, 704, 870, 884, 1029, 1572
BstDSI CCRYGG 3 cut(s) 1189, 1293, 1459
BstENI CCTNNNNNAGG 1 cut(s) 63
BstF5I GGATG 3 cut(s) 691, 1417, 1472
BstKTI GATC 8 cut(s) 139, 738, 763, 1312, 1498, 1515, 1541, 1631
BstMAI GTCTC 1 cut(s) 810
BstMBI GATC 8 cut(s) 136, 735, 760, 1309, 1495, 1512, 1538, 1628
BstMWI GCNNNNNNNGC 2 cut(s) 719, 1229
BstNI CCWGG 3 cut(s) 66, 1359, 1560
BstSCI CCNGG 4 cut(s) 64, 1357, 1500, 1558
BstSFI CTRYAG 2 cut(s) 726, 1099
BstSLI GKGCMC 1 cut(s) 197
BstSNI TACGTA 1 cut(s) 125
BstV1I GCAGC 1 cut(s) 7
BstV2I GAAGAC 1 cut(s) 510
BstX2I RGATCY 3 cut(s) 760, 1495, 1538
BstYI RGATCY 3 cut(s) 760, 1495, 1538
Bsu15I ATCGAT 1 cut(s) 818
Bsu36I CCTNAGG 1 cut(s) 1572
BsuI GTATCC 1 cut(s) 392
BsuRI GGCC 4 cut(s) 897, 1097, 1577, 1608
BsuTUI ATCGAT 1 cut(s) 818
BtgI CCRYGG 3 cut(s) 1189, 1293, 1459
BtsCI GGATG 3 cut(s) 691, 1417, 1472
BtsIMutI CAGTG 5 cut(s) 412, 439, 542, 723, 808
BveI ACCTGC 1 cut(s) 1587
Cac8I GCNNGC 2 cut(s) 1079, 1636
Cfr13I GGNCC 4 cut(s) 896, 1175, 1270, 1563
ClaI ATCGAT 1 cut(s) 818
CseI GACGC 1 cut(s) 337
Csp6I GTAC 3 cut(s) 122, 324, 1016
CviAII CATG 6 cut(s) 307, 469, 719, 1190, 1294, 1460
CviQI GTAC 3 cut(s) 122, 324, 1016
DdeI CTNAG 6 cut(s) 475, 704, 870, 884, 1029, 1572
DpnI GATC 8 cut(s) 138, 737, 762, 1311, 1497, 1514, 1540, 1630
DpnII GATC 8 cut(s) 136, 735, 760, 1309, 1495, 1512, 1538, 1628
DraI TTTAAA 1 cut(s) 1384
Eam1104I CTCTTC 1 cut(s) 751
EarI CTCTTC 1 cut(s) 751
EciI GGCGGA 1 cut(s) 1462
Eco105I TACGTA 1 cut(s) 125
Eco130I CCWWGG 4 cut(s) 385, 1189, 1293, 1459
Eco147I AGGCCT 1 cut(s) 1097
Eco31I GGTCTC 1 cut(s) 810
Eco47I GGWCC 3 cut(s) 1175, 1270, 1563
Eco81I CCTNAGG 1 cut(s) 1572
Eco88I CYCGRG 2 cut(s) 628, 1347
EcoNI CCTNNNNNAGG 1 cut(s) 63
EcoO109I RGGNCCY 2 cut(s) 1175, 1270
EcoRI GAATTC 1 cut(s) 437
EcoRII CCWGG 3 cut(s) 64, 1357, 1558
EcoT14I CCWWGG 4 cut(s) 385, 1189, 1293, 1459
EcoT22I ATGCAT 2 cut(s) 562, 1420
ErhI CCWWGG 4 cut(s) 385, 1189, 1293, 1459
FaeI CATG 6 cut(s) 310, 472, 722, 1193, 1297, 1463
FalI AAGNNNNNCTT 2 cut(s) 279, 311
FaqI GGGAC 4 cut(s) 406, 1171, 1283, 1576
FatI CATG 6 cut(s) 306, 468, 718, 1189, 1293, 1459
FauI CCCGC 1 cut(s) 383
FauNDI CATATG 1 cut(s) 490
FblI GTMKAC 1 cut(s) 278
Fnu4HI GCNGC 2 cut(s) 21, 332
FokI GGATG 3 cut(s) 678, 1424, 1479
Fsp4HI GCNGC 2 cut(s) 21, 332
FspBI CTAG 6 cut(s) 239, 311, 386, 1089, 1364, 1635
GluI GCNGC 2 cut(s) 21, 332
GsuI CTGGAG 1 cut(s) 320
HaeIII GGCC 4 cut(s) 897, 1097, 1577, 1608
HapII CCGG 2 cut(s) 1476, 1501
HgaI GACGC 1 cut(s) 337
Hin1I GRCGYC 1 cut(s) 329
Hin1II CATG 6 cut(s) 310, 472, 722, 1193, 1297, 1463
HincII GTYRAC 1 cut(s) 279
HindII GTYRAC 1 cut(s) 279
HinfI GANTC 8 cut(s) 70, 292, 594, 777, 791, 865, 1064, 1345
HpaII CCGG 2 cut(s) 1476, 1501
HphI GGTGA 3 cut(s) 845, 929, 1548
Hpy166II GTNNAC 2 cut(s) 279, 937
Hpy188I TCNGA 7 cut(s) 94, 352, 525, 696, 760, 1032, 1411
Hpy188III TCNNGA 9 cut(s) 239, 296, 337, 423, 453, 584, 1349, 1364, 1430
Hpy8I GTNNAC 2 cut(s) 279, 937
Hpy99I CGWCG 1 cut(s) 331
HpyAV CCTTC 4 cut(s) 245, 281, 1361, 1619
HpyCH4III ACNGT 7 cut(s) 283, 323, 434, 537, 727, 1338, 1438
HpyCH4IV ACGT 6 cut(s) 96, 124, 379, 823, 951, 1525
HpyCH4V TGCA 8 cut(s) 218, 416, 560, 713, 851, 1207, 1259, 1418
HpyF10VI GCNNNNNNNGC 2 cut(s) 719, 1229
HpyF3I CTNAG 6 cut(s) 475, 704, 870, 884, 1029, 1572
HpySE526I ACGT 6 cut(s) 96, 124, 379, 823, 951, 1525
Hsp92I GRCGYC 1 cut(s) 329
Hsp92II CATG 6 cut(s) 310, 472, 722, 1193, 1297, 1463
Kzo9I GATC 8 cut(s) 136, 735, 760, 1309, 1495, 1512, 1538, 1628
LmnI GCTCC 5 cut(s) 88, 237, 339, 665, 1475
Lsp1109I GCAGC 1 cut(s) 7
LweI GCATC 8 cut(s) 547, 764, 824, 1210, 1293, 1405, 1427, 1457
MaeI CTAG 6 cut(s) 239, 311, 386, 1089, 1364, 1635
MaeII ACGT 6 cut(s) 96, 124, 379, 823, 951, 1525
MaeIII GTNAC 5 cut(s) 125, 968, 983, 1129, 1504
MalI GATC 8 cut(s) 138, 737, 762, 1311, 1497, 1514, 1540, 1630
MbiI CCGCTC 1 cut(s) 334
MboI GATC 8 cut(s) 136, 735, 760, 1309, 1495, 1512, 1538, 1628
MboII GAAGA 9 cut(s) 164, 326, 365, 515, 666, 669, 738, 1312, 1634
MfeI CAATTG 1 cut(s) 858
MflI RGATCY 3 cut(s) 760, 1495, 1538
MhlI GDGCHC 2 cut(s) 197, 234
MlyI GAGTC 4 cut(s) 785, 874, 1073, 1339
MmeI TCCRAC 5 cut(s) 154, 281, 934, 1142, 1389
Mph1103I ATGCAT 2 cut(s) 562, 1420
MroXI GAANNNNTTC 1 cut(s) 291
MseI TTAA 7 cut(s) 459, 921, 1200, 1316, 1341, 1374, 1383
MslI CAYNNNNRTG 3 cut(s) 493, 1458, 1583
MspI CCGG 2 cut(s) 1476, 1501
MspR9I CCNGG 4 cut(s) 66, 1359, 1502, 1560
MunI CAATTG 1 cut(s) 858
MvaI CCWGG 3 cut(s) 66, 1359, 1560
MwoI GCNNNNNNNGC 2 cut(s) 719, 1229
NciI CCSGG 1 cut(s) 1502
NcoI CCATGG 3 cut(s) 1189, 1293, 1459
NdeI CATATG 1 cut(s) 490
NdeII GATC 8 cut(s) 136, 735, 760, 1309, 1495, 1512, 1538, 1628
NheI GCTAGC 1 cut(s) 1634
NlaIII CATG 6 cut(s) 310, 472, 722, 1193, 1297, 1463
NlaIV GGNNCC 4 cut(s) 194, 1177, 1271, 1564
NmuCI GTSAC 3 cut(s) 968, 1129, 1504
NsiI ATGCAT 2 cut(s) 562, 1420
NspV TTCGAA 1 cut(s) 1612
PaeR7I CTCGAG 1 cut(s) 1347
PceI AGGCCT 1 cut(s) 1097
PdmI GAANNNNTTC 1 cut(s) 291
PfeI GAWTC 4 cut(s) 70, 292, 594, 777
PflMI CCANNNNNTGG 2 cut(s) 590, 1449
PkrI GCNGC 2 cut(s) 22, 333
PleI GAGTC 4 cut(s) 785, 873, 1072, 1339
PpsI GAGTC 4 cut(s) 785, 873, 1072, 1339
Ppu21I YACGTR 3 cut(s) 125, 824, 1526
PpuMI RGGWCCY 2 cut(s) 1175, 1270
Psp5II RGGWCCY 2 cut(s) 1175, 1270
Psp6I CCWGG 3 cut(s) 64, 1357, 1558
PspGI CCWGG 3 cut(s) 64, 1357, 1558
PspN4I GGNNCC 4 cut(s) 194, 1177, 1271, 1564
PspPI GGNCC 4 cut(s) 896, 1175, 1270, 1563
PspPPI RGGWCCY 2 cut(s) 1175, 1270
PsuI RGATCY 3 cut(s) 760, 1495, 1538
RsaI GTAC 3 cut(s) 123, 325, 1017
RsaNI GTAC 3 cut(s) 122, 324, 1016
RseI CAYNNNNRTG 3 cut(s) 493, 1458, 1583
SalI GTCGAC 1 cut(s) 277
SaqAI TTAA 7 cut(s) 459, 921, 1200, 1316, 1341, 1374, 1383
SatI GCNGC 2 cut(s) 21, 332
Sau3AI GATC 8 cut(s) 136, 735, 760, 1309, 1495, 1512, 1538, 1628
Sau96I GGNCC 4 cut(s) 896, 1175, 1270, 1563
SchI GAGTC 4 cut(s) 785, 874, 1073, 1339
ScrFI CCNGG 4 cut(s) 66, 1359, 1502, 1560
SduI GDGCHC 2 cut(s) 197, 234
SfaNI GCATC 8 cut(s) 547, 764, 824, 1210, 1293, 1405, 1427, 1457
SfcI CTRYAG 2 cut(s) 726, 1099
Sfr274I CTCGAG 1 cut(s) 1347
SfuI TTCGAA 1 cut(s) 1612
SinI GGWCC 3 cut(s) 1175, 1270, 1563
SlaI CTCGAG 1 cut(s) 1347
SmiMI CAYNNNNRTG 3 cut(s) 493, 1458, 1583
SmlI CTYRAG 2 cut(s) 421, 1347
SmoI CTYRAG 2 cut(s) 421, 1347
SnaBI TACGTA 1 cut(s) 125
SseBI AGGCCT 1 cut(s) 1097
SsiI CCGC 3 cut(s) 332, 390, 1473
SspMI CTAG 6 cut(s) 239, 311, 386, 1089, 1364, 1635
StuI AGGCCT 1 cut(s) 1097
StyD4I CCNGG 4 cut(s) 64, 1357, 1500, 1558
StyI CCWWGG 4 cut(s) 385, 1189, 1293, 1459
TaaI ACNGT 7 cut(s) 283, 323, 434, 537, 727, 1338, 1438
TaiI ACGT 6 cut(s) 99, 127, 382, 826, 954, 1528
TaqI TCGA 7 cut(s) 278, 285, 818, 1056, 1348, 1429, 1612
TauI GCSGC 1 cut(s) 334
TfiI GAWTC 4 cut(s) 70, 292, 594, 777
Tru1I TTAA 7 cut(s) 459, 921, 1200, 1316, 1341, 1374, 1383
Tru9I TTAA 7 cut(s) 459, 921, 1200, 1316, 1341, 1374, 1383
TscAI CASTG 5 cut(s) 412, 439, 542, 730, 815
TseFI GTSAC 3 cut(s) 968, 1129, 1504
TseI GCWGC 1 cut(s) 20
Tsp45I GTSAC 3 cut(s) 968, 1129, 1504
TspDTI ATGAA 8 cut(s) 62, 89, 295, 485, 507, 707, 1415, 1542
TspGWI ACGGA 2 cut(s) 509, 753
TspRI CASTG 5 cut(s) 412, 439, 542, 730, 815
Van91I CCANNNNNTGG 2 cut(s) 590, 1449
VpaK11BI GGWCC 3 cut(s) 1175, 1270, 1563
XagI CCTNNNNNAGG 1 cut(s) 63
XapI RAATTY 5 cut(s) 116, 301, 437, 1138, 1244
XbaI TCTAGA 2 cut(s) 238, 1363
XhoI CTCGAG 1 cut(s) 1347
XmaJI CCTAGG 1 cut(s) 385
XmiI GTMKAC 1 cut(s) 278
XmnI GAANNNNTTC 1 cut(s) 291
XspI CTAG 6 cut(s) 239, 311, 386, 1089, 1364, 1635
Zsp2I ATGCAT 2 cut(s) 562, 1420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.