pycom10g23300

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000533
Physical Location & Seq
Forward (+)
25495394 .. 25496769
1376 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g23300.1

Sequence Viewer

Length: 621 bp
ATGCAGATGCAGGAATCAACAGTGTCAGCTATACTACTGGTGTTACTCCTCTATGTTCTTCATCTTCAATCAGGGGTTCACTCGCTAGCCCCTCCTCATGCTCACTATAATTTTGGTTACTTGAAATTTGTGTACAATGCTACTGATCTTCCGTTAGGAAAAAAATACGACTTCATTGTAGTTGGAGGGGGCACATCAGGGTGTCCATCGGCAGCAACTTTATCTGCAAAATACTCAGTGCTCGTTCTAGAAAGGGGCAGCCTTCCTACAGCATATCCGAACTTATTGAATCAAGATGGGTTTCTATACAATCTCCAGCAAGAAGATGATGGAAAGACACCGGTTCAAAGGATCATGTCTGAAGATGGCATTCCTACCGTAAGAGGCAGGATCATAGGGGTCATGAGCATGATCAACGCCGGCATCCATGCTCGAGCTAACTATCATTCTATAATGAGTCAGGAATTGAATGGGACATGGATTTGGTTAATAAGACATATAAGTCGGTTGAAAACACTATTGTGGGTAGGCCAAATAATTCGGTCAGTGGTTGGCAATCTGTTGCGCAAAAAGTATTTTTGGAGGCTGGTGGATATGATCCAAACAACGGATTCGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko01110 map00460 map01110
Pfam Domains
Protein Families
GMC_oxred_C GMC_oxred_N

Protein Analysis

207

Amino Acids

23.09

Weight (kDa)

9.35

Isoelectric Point (pI)

42.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 501
Acc16I TGCGCA 1 cut(s) 566
AclWI GGATC 3 cut(s) 359, 398, 592
AcsI RAATTY 1 cut(s) 125
AcuI CTGAAG 1 cut(s) 381
AfaI GTAC 1 cut(s) 134
AfiI CCNNNNNNNGG 1 cut(s) 607
AgeI ACCGGT 1 cut(s) 340
AgsI TTSAA 6 cut(s) 68, 124, 289, 347, 469, 511
AleI CACNNNNGTG 1 cut(s) 520
AluBI AGCT 2 cut(s) 29, 437
AluI AGCT 2 cut(s) 29, 437
Alw21I GWGCWC 1 cut(s) 243
AlwI GGATC 3 cut(s) 359, 398, 592
Ama87I CYCGRG 1 cut(s) 432
AoxI GGCC 1 cut(s) 529
ApeKI GCWGC 2 cut(s) 212, 258
ApoI RAATTY 1 cut(s) 125
AsiGI ACCGGT 1 cut(s) 340
AspLEI GCGC 1 cut(s) 567
AsuNHI GCTAGC 1 cut(s) 85
AvaI CYCGRG 1 cut(s) 432
BaeGI GKGCMC 1 cut(s) 194
Bbv12I GWGCWC 1 cut(s) 243
BbvI GCAGC 2 cut(s) 224, 270
BccI CCATC 4 cut(s) 214, 290, 323, 359
BclI TGATCA 1 cut(s) 411
BfaI CTAG 2 cut(s) 86, 248
BfmI CTRYAG 1 cut(s) 267
BisI GCNGC 2 cut(s) 213, 259
BlsI GCNGC 2 cut(s) 214, 260
BmeT110I CYCGRG 1 cut(s) 432
BmsI GCATC 1 cut(s) 432
BmtI GCTAGC 1 cut(s) 89
BpmI CTGGAG 1 cut(s) 299
BsaWI WCCGGW 1 cut(s) 340
Bsc4I CCNNNNNNNGG 1 cut(s) 607
Bse118I RCCGGY 2 cut(s) 340, 419
Bse1I ACTGG 1 cut(s) 42
BseGI GGATG 1 cut(s) 423
BseLI CCNNNNNNNGG 1 cut(s) 607
BseMII CTCAG 1 cut(s) 249
BseNI ACTGG 1 cut(s) 42
BseRI GAGGAG 2 cut(s) 38, 84
BseSI GKGCMC 1 cut(s) 194
BseXI GCAGC 2 cut(s) 224, 270
BshFI GGCC 1 cut(s) 531
BshTI ACCGGT 1 cut(s) 340
BsiHKAI GWGCWC 1 cut(s) 243
BsiHKCI CYCGRG 1 cut(s) 432
BsiSI CCGG 2 cut(s) 341, 420
BslFI GGGAC 1 cut(s) 487
BslI CCNNNNNNNGG 1 cut(s) 607
BsmFI GGGAC 1 cut(s) 487
BsmI GAATGC 1 cut(s) 369
BsnI GGCC 1 cut(s) 531
BsoBI CYCGRG 1 cut(s) 432
Bsp1286I GDGCHC 2 cut(s) 194, 243
Bsp1407I TGTACA 1 cut(s) 132
Bsp143I GATC 5 cut(s) 145, 351, 390, 411, 597
BspANI GGCC 1 cut(s) 531
BspCNI CTCAG 1 cut(s) 248
BspHI TCATGA 1 cut(s) 402
BspOI GCTAGC 1 cut(s) 89
BspPI GGATC 3 cut(s) 359, 398, 592
BsrFI RCCGGY 2 cut(s) 340, 419
BsrGI TGTACA 1 cut(s) 132
BsrI ACTGG 1 cut(s) 42
BssAI RCCGGY 2 cut(s) 340, 419
BssMI GATC 5 cut(s) 145, 351, 390, 411, 597
Bst4CI ACNGT 2 cut(s) 22, 379
BstAUI TGTACA 1 cut(s) 132
BstC8I GCNNGC 2 cut(s) 87, 421
BstDEI CTNAG 1 cut(s) 235
BstF5I GGATG 1 cut(s) 423
BstHHI GCGC 1 cut(s) 567
BstKTI GATC 5 cut(s) 148, 354, 393, 414, 600
BstMBI GATC 5 cut(s) 145, 351, 390, 411, 597
BstSFI CTRYAG 1 cut(s) 267
BstSLI GKGCMC 1 cut(s) 194
BstV1I GCAGC 2 cut(s) 224, 270
BsuRI GGCC 1 cut(s) 531
BtsCI GGATG 1 cut(s) 423
BtsIMutI CAGTG 3 cut(s) 27, 243, 552
Cac8I GCNNGC 2 cut(s) 87, 421
CciI TCATGA 1 cut(s) 402
CfoI GCGC 1 cut(s) 567
Cfr10I RCCGGY 2 cut(s) 340, 419
Csp6I GTAC 1 cut(s) 133
CspAI ACCGGT 1 cut(s) 340
CviAII CATG 6 cut(s) 98, 355, 403, 409, 428, 477
CviJI RGCY 6 cut(s) 29, 89, 261, 437, 531, 586
CviKI_1 RGCY 6 cut(s) 29, 89, 261, 437, 531, 586
CviQI GTAC 1 cut(s) 133
DdeI CTNAG 1 cut(s) 235
DpnI GATC 5 cut(s) 147, 353, 392, 413, 599
DpnII GATC 5 cut(s) 145, 351, 390, 411, 597
DrdI GACNNNNNNGTC 1 cut(s) 501
DseDI GACNNNNNNGTC 1 cut(s) 501
Eco57I CTGAAG 1 cut(s) 381
Eco88I CYCGRG 1 cut(s) 432
FaeI CATG 6 cut(s) 101, 358, 406, 412, 431, 480
FaqI GGGAC 1 cut(s) 487
FatI CATG 6 cut(s) 97, 354, 402, 408, 427, 476
FbaI TGATCA 1 cut(s) 411
Fnu4HI GCNGC 2 cut(s) 213, 259
FokI GGATG 1 cut(s) 410
Fsp4HI GCNGC 2 cut(s) 213, 259
FspBI CTAG 2 cut(s) 86, 248
FspI TGCGCA 1 cut(s) 566
GlaI GCGC 1 cut(s) 566
GluI GCNGC 2 cut(s) 213, 259
GsuI CTGGAG 1 cut(s) 299
HaeIII GGCC 1 cut(s) 531
HapII CCGG 2 cut(s) 341, 420
HhaI GCGC 1 cut(s) 567
Hin1II CATG 6 cut(s) 101, 358, 406, 412, 431, 480
Hin6I GCGC 1 cut(s) 565
HinP1I GCGC 1 cut(s) 565
HinfI GANTC 4 cut(s) 14, 289, 457, 611
HpaII CCGG 2 cut(s) 341, 420
Hpy166II GTNNAC 2 cut(s) 79, 133
Hpy188I TCNGA 2 cut(s) 279, 361
Hpy188III TCNNGA 4 cut(s) 248, 293, 403, 461
Hpy8I GTNNAC 2 cut(s) 79, 133
HpyAV CCTTC 1 cut(s) 272
HpyCH4III ACNGT 2 cut(s) 22, 379
HpyCH4V TGCA 3 cut(s) 4, 10, 227
HpyF3I CTNAG 1 cut(s) 235
Hsp92II CATG 6 cut(s) 101, 358, 406, 412, 431, 480
HspAI GCGC 1 cut(s) 565
KroI GCCGGC 1 cut(s) 419
KroNI GCCGGC 1 cut(s) 421
Ksp22I TGATCA 1 cut(s) 411
Kzo9I GATC 5 cut(s) 145, 351, 390, 411, 597
LpnPI CCDG 9 cut(s) 23, 57, 183, 329, 354, 373, 433, 446, 572
Lsp1109I GCAGC 2 cut(s) 224, 270
LweI GCATC 1 cut(s) 432
MaeI CTAG 2 cut(s) 86, 248
MaeIII GTNAC 2 cut(s) 42, 116
MalI GATC 5 cut(s) 147, 353, 392, 413, 599
MboI GATC 5 cut(s) 145, 351, 390, 411, 597
MboII GAAGA 5 cut(s) 50, 56, 140, 335, 374
MhlI GDGCHC 2 cut(s) 194, 243
MluCI AATT 4 cut(s) 109, 125, 464, 537
MlyI GAGTC 1 cut(s) 466
MmeI TCCRAC 1 cut(s) 163
MnlI CCTC 6 cut(s) 59, 102, 105, 179, 377, 576
MroNI GCCGGC 1 cut(s) 419
MseI TTAA 1 cut(s) 488
MslI CAYNNNNRTG 3 cut(s) 199, 407, 520
MspI CCGG 2 cut(s) 341, 420
Mva1269I GAATGC 1 cut(s) 369
NaeI GCCGGC 1 cut(s) 421
NdeII GATC 5 cut(s) 145, 351, 390, 411, 597
NgoMIV GCCGGC 1 cut(s) 419
NheI GCTAGC 1 cut(s) 85
NlaIII CATG 6 cut(s) 101, 358, 406, 412, 431, 480
NsbI TGCGCA 1 cut(s) 566
OliI CACNNNNGTG 1 cut(s) 520
PaeR7I CTCGAG 1 cut(s) 432
PagI TCATGA 1 cut(s) 402
PctI GAATGC 1 cut(s) 369
PdiI GCCGGC 1 cut(s) 421
PfeI GAWTC 3 cut(s) 14, 289, 611
PinAI ACCGGT 1 cut(s) 340
PkrI GCNGC 2 cut(s) 214, 260
PleI GAGTC 1 cut(s) 465
PpsI GAGTC 1 cut(s) 465
PspXI VCTCGAGB 1 cut(s) 432
RsaI GTAC 1 cut(s) 134
RsaNI GTAC 1 cut(s) 133
RseI CAYNNNNRTG 3 cut(s) 199, 407, 520
SaqAI TTAA 1 cut(s) 488
SatI GCNGC 2 cut(s) 213, 259
Sau3AI GATC 5 cut(s) 145, 351, 390, 411, 597
SchI GAGTC 1 cut(s) 466
SduI GDGCHC 2 cut(s) 194, 243
SetI ASST 2 cut(s) 31, 439
SfaNI GCATC 1 cut(s) 432
SfcI CTRYAG 1 cut(s) 267
Sfr274I CTCGAG 1 cut(s) 432
SlaI CTCGAG 1 cut(s) 432
SmiMI CAYNNNNRTG 3 cut(s) 199, 407, 520
SmlI CTYRAG 1 cut(s) 432
SmoI CTYRAG 1 cut(s) 432
Sse9I AATT 4 cut(s) 109, 125, 464, 537
SspMI CTAG 2 cut(s) 86, 248
TaaI ACNGT 2 cut(s) 22, 379
TaqI TCGA 1 cut(s) 433
TaqII GACCGA 1 cut(s) 531
TasI AATT 4 cut(s) 109, 125, 464, 537
TatI WGTACW 1 cut(s) 132
TfiI GAWTC 3 cut(s) 14, 289, 611
Tru1I TTAA 1 cut(s) 488
Tru9I TTAA 1 cut(s) 488
TscAI CASTG 3 cut(s) 27, 243, 552
TseI GCWGC 2 cut(s) 212, 258
TspDTI ATGAA 2 cut(s) 50, 163
TspGWI ACGGA 1 cut(s) 141
TspRI CASTG 3 cut(s) 27, 243, 552
XapI RAATTY 1 cut(s) 125
XbaI TCTAGA 1 cut(s) 247
XhoI CTCGAG 1 cut(s) 432
XspI CTAG 2 cut(s) 86, 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.