Rroxscaffold_5G00348350

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000533
Physical Location & Seq
Reverse (-)
20467257 .. 20472380
5124 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00348350.1

Sequence Viewer

Length: 1251 bp
ATGATCAATGCTGGTTTCTACTCCAAAGCTGACAGGGAATTCTTTGTTAAATCTGGAATTAAATGGGACATGGATTTGGTTGATAAGGCATATCACTGGGTTGAAAGCACTGTTGTCTCTCGTCCAAATCTGTCACGTTCAAATTGGCAATCTATTGTGAAAGAAGCATTGTTGGAGGCTGGTGTTGGTCCAAACAATGGATTCAGTTTGGATCACATTAAGGGAACTAAAGTTGGCGGTTCTACTTTTGATACACATGGAAGGAGACACGGAGCTGTTGAATTGCTTAACAGAGGAAACCTAAAGAACTTGCAAGTGGGAATTTATGCCACAGTCGAGAAAATCATCTTCTCTTCCAAAGAATCAGGTGTTTCGGCTCGAGGGGTGGTATATACTGATTCTAATGGGAGGTCTCATCGGGCATTTGTACGTGGTAAGGGAGAGGTTATTCTGAGTGCTGGGGCAATTGGGAGTCCTCAGCTTCTACTTCTTAGCGGTGTTGGCCCTCAGTCCTATCTATCATCTTTGAAAATCCCAGTTGTTCACCCCCAACCTCACACTGGAAACTTTATGTATGACAACCCTCGTAATTTCGTTACCATTTTATCCCCGTTTCCACTACAGGCCTCAACTGTAAAGATTGTCGGCATTACCGACCATTTCTACGTAGAGACCTTGTCTAGCATGCCCATTTCTACTACACCCTACAGTGTTTTTCCAGATCCGGCAAAACTTATAAAGATAAATTCAAGTTTAGGACTCATTGCCATCAAATTAGTAGGACCTTTGTCTTATGGTACTCTGAAGCTTCAATCATCATCTGATGTGAGAGTCGGTCCAAATGTCCGCTTCAACTACTTTGCACATCCCTTGGACCTTGCTCGTTGTGTTTCTGGCATGAAGAAGATTGGGGAGTTATTAAATACCAAGTCACTGAAAGCATTTAAGGTTCAAGCTTCGTCGAGCACAAAAGCTTACAATTTTTATGGTCCAACTTTACCTATGAACCAGACAGATGATGCATCTTTTGGAGAATTTTGTCGTGATACAGTATCAACAATTTGGCATTTCCACGGAGGATGCCTTGTCGGAAAGGTGGTCGATGGCAGCTTGCGGGTCATGGGAATCGATGCTTTGCGTGTTGTTGACGCATCCACATTCAATTTCACACCGGGGACAAATCCTCAGGCCACACTTATGATGTTGGGCAGGTACATTGGCATTAAGATGCTGCAACAAAGATCTTCTTAA

Protein Analysis

416

Amino Acids

45.32

Weight (kDa)

9.64

Isoelectric Point (pI)

26.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_N PF00732 17 - 172 1.9e-12 GMC oxidoreductase
GMC_oxred_C PF05199 262 - 403 8.1e-26 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 737
Acc36I ACCTGC 1 cut(s) 1200
AccB7I CCANNNNNTGG 1 cut(s) 197
AciI CCGC 4 cut(s) 237, 495, 847, 1114
AclWI GGATC 2 cut(s) 219, 716
AcsI RAATTY 4 cut(s) 38, 321, 745, 1034
AcuI CTGAAG 1 cut(s) 824
AfaI GTAC 3 cut(s) 429, 799, 1214
AfiI CCNNNNNNNGG 2 cut(s) 197, 560
AgsI TTSAA 9 cut(s) 104, 141, 281, 529, 750, 812, 853, 953, 1162
AluBI AGCT 7 cut(s) 29, 275, 481, 808, 956, 974, 1110
AluI AGCT 7 cut(s) 29, 275, 481, 808, 956, 974, 1110
Alw21I GWGCWC 1 cut(s) 968
Alw26I GTCTC 4 cut(s) 121, 259, 417, 665
AlwI GGATC 2 cut(s) 219, 716
Ama87I CYCGRG 1 cut(s) 378
AoxI GGCC 3 cut(s) 502, 624, 1188
ApeKI GCWGC 2 cut(s) 1107, 1231
ApoI RAATTY 4 cut(s) 38, 321, 745, 1034
AspS9I GGNCC 6 cut(s) 188, 503, 782, 836, 874, 989
AsuC2I CCSGG 1 cut(s) 1173
AsuHPI GGTGA 1 cut(s) 536
AvaI CYCGRG 1 cut(s) 378
AvaII GGWCC 5 cut(s) 188, 782, 836, 874, 989
AxyI CCTNAGG 1 cut(s) 1185
Bbv12I GWGCWC 1 cut(s) 968
BbvCI CCTCAGC 1 cut(s) 477
BbvI GCAGC 2 cut(s) 1119, 1218
BccI CCATC 2 cut(s) 776, 1097
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 1 cut(s) 1173
BcoDI GTCTC 4 cut(s) 121, 259, 417, 665
BfaI CTAG 1 cut(s) 681
BfmI CTRYAG 2 cut(s) 620, 706
BfuAI ACCTGC 1 cut(s) 1200
BglII AGATCT 1 cut(s) 1241
BisI GCNGC 2 cut(s) 1108, 1232
BlsI GCNGC 2 cut(s) 1109, 1233
Bme1390I CCNGG 1 cut(s) 1173
Bme18I GGWCC 5 cut(s) 188, 782, 836, 874, 989
BmeT110I CYCGRG 1 cut(s) 378
BmgT120I GGNCC 6 cut(s) 188, 503, 782, 836, 874, 989
BmrFI CCNGG 1 cut(s) 1173
BmrI ACTGGG 2 cut(s) 106, 530
BmsI GCATC 6 cut(s) 1009, 1031, 1070, 1120, 1160, 1218
BmuI ACTGGG 2 cut(s) 106, 530
BoxI GACNNNNGTC 1 cut(s) 787
Bpu10I CCTNAGC 1 cut(s) 477
BpuMI CCSGG 1 cut(s) 1173
Bsa29I ATCGAT 1 cut(s) 1128
BsaAI YACGTR 2 cut(s) 431, 667
BsaI GGTCTC 2 cut(s) 417, 665
BsaJI CCNNGG 3 cut(s) 870, 1072, 1172
Bsc4I CCNNNNNNNGG 2 cut(s) 197, 560
Bse1I ACTGG 3 cut(s) 101, 536, 565
Bse21I CCTNAGG 1 cut(s) 1185
Bse3DI GCAATG 1 cut(s) 762
BseCI ATCGAT 1 cut(s) 1128
BseDI CCNNGG 3 cut(s) 870, 1072, 1172
BseGI GGATG 3 cut(s) 865, 1085, 1151
BseLI CCNNNNNNNGG 2 cut(s) 197, 560
BseMI GCAATG 1 cut(s) 762
BseMII CTCAG 4 cut(s) 443, 491, 521, 1199
BseNI ACTGG 3 cut(s) 101, 536, 565
BseXI GCAGC 2 cut(s) 1119, 1218
BseYI CCCAGC 1 cut(s) 458
BshFI GGCC 3 cut(s) 504, 626, 1190
BshVI ATCGAT 1 cut(s) 1128
BsiHKAI GWGCWC 1 cut(s) 968
BsiHKCI CYCGRG 1 cut(s) 378
BsiSI CCGG 2 cut(s) 725, 1172
BslFI GGGAC 2 cut(s) 80, 1189
BslI CCNNNNNNNGG 2 cut(s) 197, 560
BsmAI GTCTC 4 cut(s) 121, 259, 417, 665
BsmFI GGGAC 2 cut(s) 80, 1189
BsnI GGCC 3 cut(s) 504, 626, 1190
Bso31I GGTCTC 2 cut(s) 417, 665
BsoBI CYCGRG 1 cut(s) 378
Bsp1286I GDGCHC 1 cut(s) 968
Bsp143I GATC 4 cut(s) 3, 211, 721, 1241
BspACI CCGC 4 cut(s) 237, 495, 847, 1114
BspANI GGCC 3 cut(s) 504, 626, 1190
BspCNI CTCAG 4 cut(s) 444, 490, 520, 1198
BspDI ATCGAT 1 cut(s) 1128
BspMI ACCTGC 1 cut(s) 1200
BspPI GGATC 2 cut(s) 219, 716
BspTNI GGTCTC 2 cut(s) 417, 665
BsrDI GCAATG 1 cut(s) 762
BsrI ACTGG 3 cut(s) 101, 536, 565
BssECI CCNNGG 3 cut(s) 870, 1072, 1172
BssMI GATC 4 cut(s) 3, 211, 721, 1241
BssT1I CCWWGG 1 cut(s) 870
Bst4CI ACNGT 5 cut(s) 112, 334, 634, 710, 1051
Bst6I CTCTTC 1 cut(s) 358
BstBAI YACGTR 2 cut(s) 431, 667
BstC8I GCNNGC 2 cut(s) 686, 1112
BstDEI CTNAG 5 cut(s) 452, 477, 491, 507, 1185
BstDSI CCRYGG 1 cut(s) 1072
BstF5I GGATG 3 cut(s) 865, 1085, 1151
BstKTI GATC 4 cut(s) 6, 214, 724, 1244
BstMAI GTCTC 4 cut(s) 121, 259, 417, 665
BstMBI GATC 4 cut(s) 3, 211, 721, 1241
BstMWI GCNNNNNNNGC 1 cut(s) 501
BstNSI RCATGY 1 cut(s) 688
BstPAI GACNNNNGTC 1 cut(s) 787
BstSCI CCNGG 1 cut(s) 1171
BstSFI CTRYAG 2 cut(s) 620, 706
BstSNI TACGTA 1 cut(s) 667
BstV1I GCAGC 2 cut(s) 1119, 1218
BstX2I RGATCY 2 cut(s) 721, 1241
BstYI RGATCY 2 cut(s) 721, 1241
Bsu15I ATCGAT 1 cut(s) 1128
Bsu36I CCTNAGG 1 cut(s) 1185
BsuRI GGCC 3 cut(s) 504, 626, 1190
BsuTUI ATCGAT 1 cut(s) 1128
BtgI CCRYGG 1 cut(s) 1072
BtsCI GGATG 3 cut(s) 865, 1085, 1151
BtsIMutI CAGTG 5 cut(s) 94, 108, 558, 715, 932
BveI ACCTGC 1 cut(s) 1200
Cac8I GCNNGC 2 cut(s) 686, 1112
Cfr13I GGNCC 6 cut(s) 188, 503, 782, 836, 874, 989
ClaI ATCGAT 1 cut(s) 1128
CseI GACGC 1 cut(s) 1157
Csp6I GTAC 3 cut(s) 428, 798, 1213
CviAII CATG 5 cut(s) 70, 257, 685, 898, 1120
CviQI GTAC 3 cut(s) 428, 798, 1213
DdeI CTNAG 5 cut(s) 452, 477, 491, 507, 1185
DpnI GATC 4 cut(s) 5, 213, 723, 1243
DpnII GATC 4 cut(s) 3, 211, 721, 1241
Eam1104I CTCTTC 1 cut(s) 358
EarI CTCTTC 1 cut(s) 358
Eco105I TACGTA 1 cut(s) 667
Eco130I CCWWGG 1 cut(s) 870
Eco147I AGGCCT 1 cut(s) 626
Eco31I GGTCTC 2 cut(s) 417, 665
Eco47I GGWCC 5 cut(s) 188, 782, 836, 874, 989
Eco57I CTGAAG 1 cut(s) 824
Eco81I CCTNAGG 1 cut(s) 1185
Eco88I CYCGRG 1 cut(s) 378
EcoO109I RGGNCCY 1 cut(s) 782
EcoRI GAATTC 1 cut(s) 38
EcoT14I CCWWGG 1 cut(s) 870
EcoT22I ATGCAT 1 cut(s) 1024
ErhI CCWWGG 1 cut(s) 870
FaeI CATG 5 cut(s) 73, 260, 688, 901, 1123
FalI AAGNNNNNCTT 1 cut(s) 1231
FaqI GGGAC 2 cut(s) 80, 1189
FatI CATG 5 cut(s) 69, 256, 684, 897, 1119
FauI CCCGC 1 cut(s) 1107
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 2 cut(s) 1108, 1232
FokI GGATG 3 cut(s) 852, 1092, 1138
Fsp4HI GCNGC 2 cut(s) 1108, 1232
FspBI CTAG 1 cut(s) 681
GluI GCNGC 2 cut(s) 1108, 1232
GsaI CCCAGC 1 cut(s) 462
HaeIII GGCC 3 cut(s) 504, 626, 1190
HapII CCGG 2 cut(s) 725, 1172
HgaI GACGC 1 cut(s) 1157
Hin1II CATG 5 cut(s) 73, 260, 688, 901, 1123
HincII GTYRAC 1 cut(s) 1147
HindII GTYRAC 1 cut(s) 1147
HindIII AAGCTT 3 cut(s) 806, 954, 972
HinfI GANTC 7 cut(s) 201, 362, 398, 472, 759, 831, 1125
HpaII CCGG 2 cut(s) 725, 1172
HphI GGTGA 1 cut(s) 536
Hpy166II GTNNAC 2 cut(s) 544, 1147
Hpy188I TCNGA 4 cut(s) 453, 804, 823, 1091
Hpy188III TCNNGA 4 cut(s) 54, 337, 719, 1043
Hpy8I GTNNAC 2 cut(s) 544, 1147
Hpy99I CGWCG 1 cut(s) 964
HpyAV CCTTC 1 cut(s) 255
HpyCH4III ACNGT 5 cut(s) 112, 334, 634, 710, 1051
HpyCH4IV ACGT 3 cut(s) 136, 430, 666
HpyCH4V TGCA 4 cut(s) 313, 863, 1022, 1234
HpyF10VI GCNNNNNNNGC 1 cut(s) 501
HpyF3I CTNAG 5 cut(s) 452, 477, 491, 507, 1185
HpySE526I ACGT 3 cut(s) 136, 430, 666
Hsp92II CATG 5 cut(s) 73, 260, 688, 901, 1123
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 4 cut(s) 3, 211, 721, 1241
LmnI GCTCC 1 cut(s) 272
Lsp1109I GCAGC 2 cut(s) 1119, 1218
LweI GCATC 6 cut(s) 1009, 1031, 1070, 1120, 1160, 1218
MaeI CTAG 1 cut(s) 681
MaeII ACGT 3 cut(s) 136, 430, 666
MaeIII GTNAC 3 cut(s) 132, 595, 930
MalI GATC 4 cut(s) 5, 213, 723, 1243
MboI GATC 4 cut(s) 3, 211, 721, 1241
MboII GAAGA 5 cut(s) 340, 345, 913, 916, 1236
MfeI CAATTG 1 cut(s) 465
MflI RGATCY 2 cut(s) 721, 1241
MhlI GDGCHC 1 cut(s) 968
MlyI GAGTC 3 cut(s) 481, 753, 840
MmeI TCCRAC 3 cut(s) 153, 1016, 1069
Mph1103I ATGCAT 1 cut(s) 1024
MseI TTAA 8 cut(s) 48, 60, 219, 288, 920, 945, 1224, 1249
MslI CAYNNNNRTG 2 cut(s) 1196, 1226
MspI CCGG 2 cut(s) 725, 1172
MspR9I CCNGG 1 cut(s) 1173
MunI CAATTG 1 cut(s) 465
MwoI GCNNNNNNNGC 1 cut(s) 501
NciI CCSGG 1 cut(s) 1173
NdeII GATC 4 cut(s) 3, 211, 721, 1241
NlaIII CATG 5 cut(s) 73, 260, 688, 901, 1123
NmuCI GTSAC 2 cut(s) 132, 930
NsiI ATGCAT 1 cut(s) 1024
NspI RCATGY 1 cut(s) 688
PaeI GCATGC 1 cut(s) 688
PaeR7I CTCGAG 1 cut(s) 378
PceI AGGCCT 1 cut(s) 626
PcsI WCGNNNNNNNCGW 1 cut(s) 651
PfeI GAWTC 4 cut(s) 201, 362, 398, 1125
PflFI GACNNNGTC 1 cut(s) 676
PflMI CCANNNNNTGG 1 cut(s) 197
PkrI GCNGC 2 cut(s) 1109, 1233
PleI GAGTC 3 cut(s) 480, 753, 839
PpsI GAGTC 3 cut(s) 480, 753, 839
Ppu21I YACGTR 2 cut(s) 431, 667
PpuMI RGGWCCY 1 cut(s) 782
PshAI GACNNNNGTC 1 cut(s) 787
PsiI TTATAA 1 cut(s) 737
Psp5II RGGWCCY 1 cut(s) 782
PspFI CCCAGC 1 cut(s) 458
PspPI GGNCC 6 cut(s) 188, 503, 782, 836, 874, 989
PspPPI RGGWCCY 1 cut(s) 782
PspXI VCTCGAGB 1 cut(s) 378
PsuI RGATCY 2 cut(s) 721, 1241
PsyI GACNNNGTC 1 cut(s) 676
RsaI GTAC 3 cut(s) 429, 799, 1214
RsaNI GTAC 3 cut(s) 428, 798, 1213
RseI CAYNNNNRTG 2 cut(s) 1196, 1226
SaqAI TTAA 8 cut(s) 48, 60, 219, 288, 920, 945, 1224, 1249
SatI GCNGC 2 cut(s) 1108, 1232
Sau3AI GATC 4 cut(s) 3, 211, 721, 1241
Sau96I GGNCC 6 cut(s) 188, 503, 782, 836, 874, 989
SchI GAGTC 3 cut(s) 481, 753, 840
ScrFI CCNGG 1 cut(s) 1173
SduI GDGCHC 1 cut(s) 968
SfaNI GCATC 6 cut(s) 1009, 1031, 1070, 1120, 1160, 1218
SfcI CTRYAG 2 cut(s) 620, 706
Sfr274I CTCGAG 1 cut(s) 378
SinI GGWCC 5 cut(s) 188, 782, 836, 874, 989
SlaI CTCGAG 1 cut(s) 378
SmiMI CAYNNNNRTG 2 cut(s) 1196, 1226
SmlI CTYRAG 1 cut(s) 378
SmoI CTYRAG 1 cut(s) 378
SnaBI TACGTA 1 cut(s) 667
SphI GCATGC 1 cut(s) 688
SseBI AGGCCT 1 cut(s) 626
SsiI CCGC 4 cut(s) 237, 495, 847, 1114
SspMI CTAG 1 cut(s) 681
StuI AGGCCT 1 cut(s) 626
StyD4I CCNGG 1 cut(s) 1171
StyI CCWWGG 1 cut(s) 870
TaaI ACNGT 5 cut(s) 112, 334, 634, 710, 1051
TaiI ACGT 3 cut(s) 139, 433, 669
TaqI TCGA 5 cut(s) 336, 379, 962, 1101, 1128
TaqII GACCGA 1 cut(s) 824
TfiI GAWTC 4 cut(s) 201, 362, 398, 1125
Tru1I TTAA 8 cut(s) 48, 60, 219, 288, 920, 945, 1224, 1249
Tru9I TTAA 8 cut(s) 48, 60, 219, 288, 920, 945, 1224, 1249
TscAI CASTG 5 cut(s) 101, 115, 565, 715, 939
TseFI GTSAC 2 cut(s) 132, 930
TseI GCWGC 2 cut(s) 1107, 1231
Tsp45I GTSAC 2 cut(s) 132, 930
TspDTI ATGAA 2 cut(s) 914, 1019
TspGWI ACGGA 2 cut(s) 285, 1089
TspRI CASTG 5 cut(s) 101, 115, 565, 715, 939
Tth111I GACNNNGTC 1 cut(s) 676
Van91I CCANNNNNTGG 1 cut(s) 197
VpaK11BI GGWCC 5 cut(s) 188, 782, 836, 874, 989
XapI RAATTY 4 cut(s) 38, 321, 745, 1034
XceI RCATGY 1 cut(s) 688
XcmI CCANNNNNNNNNTGG 1 cut(s) 557
XhoI CTCGAG 1 cut(s) 378
XspI CTAG 1 cut(s) 681
Zsp2I ATGCAT 1 cut(s) 1024
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.