MD10G1279300.v1.1

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000533
Physical Location & Seq
Forward (+)
37012809 .. 37014142
1334 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1279300.v1.1.491

Sequence Viewer

Length: 918 bp
ATGCAGGAATCATCAATGTCAGCTATACTACTGGTGTTACTCCTCTTTGTTCTTCATCTTCAATCAGGGGTTCACTCGCTAGCCCCTCCTCATGCTCACGATTTTGGTTACTTGAAATTTGTGTACAATGCTACTGATCTTCCGTTAGGAGCAAAATACGACTACATCGTAGTTGGAGGGGGCACATCAGGGTGTCCATTGGCAGCAACTTTATCTGCAAAATACTCAGTGCTCGTTCTAGAAAGGGGCAACCTTCCTACAGCATATCCGAACGTATTGAATCAAGATGGGTTTCTATACAATCTCCACCAAGAAGATGATGGAAATACACCGGTTCAAAGGATCATGTCTGAAGATGGCATTCCTACCGTAAGAGGCAGGATCCTCGGGGGCACGAGCATGATCAACGCCGGTGTCCATGCTCGAGCTAACATATCATTCTATAATGAGTCAGGAATTGAATGGGACATGGATTTGGTTAATAAGACATATAAGTGGGTTGAAAACACTATTGTGGGTAGGCCAAATAATTCGGTCGGTGGTTGGCAATCTGTTGCGCAAAAAGCATTTTTGGAGGCTGGTGGATATGATCCAAACAATGGATTCGTTTTAGATCACATAGAAGGAACTAGATTTACAGATTTGTCAGCTACAGGAGTTATATATAGGGATTCTAATGGAAGGTCTCATCGGGCACTTGTACACGATAAAGGAGAAATTATATTAAGTTCTGGGACAATTGCGTCTCCTCAACTTCTTTTACTTAGTGGTATTGGCCCAAAGTCTTACCTATCATCTCTAAAAATCCCAGTTGTTCTTGATCTTCCCGATGTTGGGCAGTTTATGTATGACAACCCTCGCAATTTCATTAACATTTTGACCCCAAATGATGTTAGGAATGTCGGTACTACAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko01110 map00460 map01110
Pfam Domains
Protein Families
GMC_oxred_C GMC_oxred_N

Protein Analysis

306

Amino Acids

33.18

Weight (kDa)

5.82

Isoelectric Point (pI)

38.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_N PF00732 199 - 280 2.2e-08 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 742
Acc16I TGCGCA 1 cut(s) 558
AccB7I CCANNNNNTGG 1 cut(s) 599
AclWI GGATC 4 cut(s) 350, 376, 389, 584
AcsI RAATTY 1 cut(s) 116
AcuI CTGAAG 1 cut(s) 372
AfaI GTAC 3 cut(s) 125, 702, 907
AfiI CCNNNNNNNGG 3 cut(s) 599, 833, 834
AgeI ACCGGT 1 cut(s) 331
AgsI TTSAA 6 cut(s) 62, 115, 280, 338, 461, 503
AleI CACNNNNGTG 1 cut(s) 512
AluBI AGCT 3 cut(s) 23, 428, 650
AluI AGCT 3 cut(s) 23, 428, 650
Alw21I GWGCWC 1 cut(s) 234
Alw26I GTCTC 2 cut(s) 690, 750
AlwI GGATC 4 cut(s) 350, 376, 389, 584
Ama87I CYCGRG 2 cut(s) 386, 423
AoxI GGCC 2 cut(s) 521, 775
ApeKI GCWGC 1 cut(s) 203
ApoI RAATTY 1 cut(s) 116
AsiGI ACCGGT 1 cut(s) 331
AspLEI GCGC 1 cut(s) 559
AspS9I GGNCC 1 cut(s) 776
AsuNHI GCTAGC 1 cut(s) 79
AvaI CYCGRG 2 cut(s) 386, 423
BaeGI GKGCMC 3 cut(s) 185, 395, 697
BamHI GGATCC 1 cut(s) 381
BauI CACGAG 1 cut(s) 394
Bbv12I GWGCWC 1 cut(s) 234
BbvI GCAGC 1 cut(s) 215
BccI CCATC 3 cut(s) 281, 314, 350
BcgI CGANNNNNNTGC 2 cut(s) 367, 401
BclI TGATCA 1 cut(s) 402
BcoDI GTCTC 2 cut(s) 690, 750
BfaI CTAG 3 cut(s) 80, 239, 630
BfmI CTRYAG 2 cut(s) 258, 651
BisI GCNGC 1 cut(s) 204
BlsI GCNGC 1 cut(s) 205
BmeT110I CYCGRG 2 cut(s) 386, 423
BmgT120I GGNCC 1 cut(s) 776
BmiI GGNNCC 1 cut(s) 383
BmrI ACTGGG 1 cut(s) 803
BmtI GCTAGC 1 cut(s) 83
BmuI ACTGGG 1 cut(s) 803
BsaI GGTCTC 1 cut(s) 690
BsaJI CCNNGG 1 cut(s) 385
BsaWI WCCGGW 1 cut(s) 331
Bsc4I CCNNNNNNNGG 3 cut(s) 599, 833, 834
Bse118I RCCGGY 2 cut(s) 331, 410
Bse1I ACTGG 2 cut(s) 36, 809
BseDI CCNNGG 1 cut(s) 385
BseLI CCNNNNNNNGG 3 cut(s) 599, 833, 834
BseMII CTCAG 1 cut(s) 240
BseNI ACTGG 2 cut(s) 36, 809
BseRI GAGGAG 3 cut(s) 32, 78, 738
BseSI GKGCMC 3 cut(s) 185, 395, 697
BseXI GCAGC 1 cut(s) 215
Bsh1285I CGRYCG 1 cut(s) 537
BshFI GGCC 2 cut(s) 523, 777
BshTI ACCGGT 1 cut(s) 331
BsiEI CGRYCG 1 cut(s) 537
BsiHKAI GWGCWC 1 cut(s) 234
BsiHKCI CYCGRG 2 cut(s) 386, 423
BsiSI CCGG 2 cut(s) 332, 411
BslFI GGGAC 2 cut(s) 479, 748
BslI CCNNNNNNNGG 3 cut(s) 599, 833, 834
BsmAI GTCTC 2 cut(s) 690, 750
BsmBI CGTCTC 1 cut(s) 750
BsmFI GGGAC 2 cut(s) 479, 748
BsmI GAATGC 1 cut(s) 360
BsnI GGCC 2 cut(s) 523, 777
Bso31I GGTCTC 1 cut(s) 690
BsoBI CYCGRG 2 cut(s) 386, 423
Bsp1286I GDGCHC 4 cut(s) 185, 234, 395, 697
Bsp1407I TGTACA 2 cut(s) 123, 700
Bsp143I GATC 7 cut(s) 136, 342, 381, 402, 589, 613, 820
BspANI GGCC 2 cut(s) 523, 777
BspCNI CTCAG 1 cut(s) 239
BspLI GGNNCC 1 cut(s) 383
BspOI GCTAGC 1 cut(s) 83
BspPI GGATC 4 cut(s) 350, 376, 389, 584
BspTNI GGTCTC 1 cut(s) 690
BsrFI RCCGGY 2 cut(s) 331, 410
BsrGI TGTACA 2 cut(s) 123, 700
BsrI ACTGG 2 cut(s) 36, 809
BssAI RCCGGY 2 cut(s) 331, 410
BssECI CCNNGG 1 cut(s) 385
BssMI GATC 7 cut(s) 136, 342, 381, 402, 589, 613, 820
BssSI CACGAG 1 cut(s) 394
Bst2BI CACGAG 1 cut(s) 394
Bst4CI ACNGT 1 cut(s) 370
BstAUI TGTACA 2 cut(s) 123, 700
BstC8I GCNNGC 1 cut(s) 81
BstDEI CTNAG 2 cut(s) 226, 764
BstHHI GCGC 1 cut(s) 559
BstKTI GATC 7 cut(s) 139, 345, 384, 405, 592, 616, 823
BstMAI GTCTC 2 cut(s) 690, 750
BstMBI GATC 7 cut(s) 136, 342, 381, 402, 589, 613, 820
BstMCI CGRYCG 1 cut(s) 537
BstMWI GCNNNNNNNGC 1 cut(s) 563
BstSFI CTRYAG 2 cut(s) 258, 651
BstSLI GKGCMC 3 cut(s) 185, 395, 697
BstV1I GCAGC 1 cut(s) 215
BstX2I RGATCY 1 cut(s) 381
BstYI RGATCY 1 cut(s) 381
BsuRI GGCC 2 cut(s) 523, 777
BtsIMutI CAGTG 1 cut(s) 234
Cac8I GCNNGC 1 cut(s) 81
CfoI GCGC 1 cut(s) 559
Cfr10I RCCGGY 2 cut(s) 331, 410
Cfr13I GGNCC 1 cut(s) 776
CseI GACGC 1 cut(s) 732
Csp6I GTAC 3 cut(s) 124, 701, 906
CspAI ACCGGT 1 cut(s) 331
CviAII CATG 5 cut(s) 92, 346, 400, 419, 469
CviJI RGCY 7 cut(s) 23, 83, 428, 523, 578, 650, 777
CviKI_1 RGCY 7 cut(s) 23, 83, 428, 523, 578, 650, 777
CviQI GTAC 3 cut(s) 124, 701, 906
DdeI CTNAG 2 cut(s) 226, 764
DpnI GATC 7 cut(s) 138, 344, 383, 404, 591, 615, 822
DpnII GATC 7 cut(s) 136, 342, 381, 402, 589, 613, 820
DrdI GACNNNNNNGTC 1 cut(s) 742
DseDI GACNNNNNNGTC 1 cut(s) 742
Eco31I GGTCTC 1 cut(s) 690
Eco57I CTGAAG 1 cut(s) 372
Eco88I CYCGRG 2 cut(s) 386, 423
Esp3I CGTCTC 1 cut(s) 750
FaeI CATG 5 cut(s) 95, 349, 403, 422, 472
FaqI GGGAC 2 cut(s) 479, 748
FatI CATG 5 cut(s) 91, 345, 399, 418, 468
FbaI TGATCA 1 cut(s) 402
Fnu4HI GCNGC 1 cut(s) 204
Fsp4HI GCNGC 1 cut(s) 204
FspBI CTAG 3 cut(s) 80, 239, 630
FspI TGCGCA 1 cut(s) 558
GlaI GCGC 1 cut(s) 558
GluI GCNGC 1 cut(s) 204
HaeIII GGCC 2 cut(s) 523, 777
HapII CCGG 2 cut(s) 332, 411
HgaI GACGC 1 cut(s) 732
HhaI GCGC 1 cut(s) 559
Hin1II CATG 5 cut(s) 95, 349, 403, 422, 472
Hin6I GCGC 1 cut(s) 557
HinP1I GCGC 1 cut(s) 557
HinfI GANTC 5 cut(s) 8, 280, 449, 603, 671
HpaII CCGG 2 cut(s) 332, 411
Hpy166II GTNNAC 3 cut(s) 73, 124, 703
Hpy188I TCNGA 2 cut(s) 270, 352
Hpy188III TCNNGA 6 cut(s) 98, 239, 284, 453, 818, 827
Hpy8I GTNNAC 3 cut(s) 73, 124, 703
HpyAV CCTTC 3 cut(s) 263, 617, 675
HpyCH4III ACNGT 1 cut(s) 370
HpyCH4IV ACGT 1 cut(s) 273
HpyCH4V TGCA 2 cut(s) 4, 218
HpyF10VI GCNNNNNNNGC 1 cut(s) 563
HpyF3I CTNAG 2 cut(s) 226, 764
HpySE526I ACGT 1 cut(s) 273
Hsp92II CATG 5 cut(s) 95, 349, 403, 422, 472
HspAI GCGC 1 cut(s) 557
Ksp22I TGATCA 1 cut(s) 402
Kzo9I GATC 7 cut(s) 136, 342, 381, 402, 589, 613, 820
LmnI GCTCC 1 cut(s) 149
Lsp1109I GCAGC 1 cut(s) 215
MaeI CTAG 3 cut(s) 80, 239, 630
MaeII ACGT 1 cut(s) 273
MaeIII GTNAC 2 cut(s) 36, 107
MalI GATC 7 cut(s) 138, 344, 383, 404, 591, 615, 822
MboI GATC 7 cut(s) 136, 342, 381, 402, 589, 613, 820
MboII GAAGA 6 cut(s) 44, 50, 131, 326, 365, 815
MfeI CAATTG 1 cut(s) 738
MflI RGATCY 1 cut(s) 381
MhlI GDGCHC 4 cut(s) 185, 234, 395, 697
MluCI AATT 6 cut(s) 116, 456, 529, 717, 738, 862
MlyI GAGTC 1 cut(s) 458
MmeI TCCRAC 1 cut(s) 154
MnlI CCTC 9 cut(s) 53, 96, 99, 170, 368, 395, 568, 759, 867
MseI TTAA 3 cut(s) 480, 725, 870
MslI CAYNNNNRTG 4 cut(s) 190, 398, 493, 512
MspI CCGG 2 cut(s) 332, 411
MunI CAATTG 1 cut(s) 738
Mva1269I GAATGC 1 cut(s) 360
MwoI GCNNNNNNNGC 1 cut(s) 563
NdeII GATC 7 cut(s) 136, 342, 381, 402, 589, 613, 820
NheI GCTAGC 1 cut(s) 79
NlaIII CATG 5 cut(s) 95, 349, 403, 422, 472
NlaIV GGNNCC 1 cut(s) 383
NsbI TGCGCA 1 cut(s) 558
OliI CACNNNNGTG 1 cut(s) 512
PaeR7I CTCGAG 1 cut(s) 423
PcsI WCGNNNNNNNCGW 1 cut(s) 165
PctI GAATGC 1 cut(s) 360
PfeI GAWTC 4 cut(s) 8, 280, 603, 671
PflMI CCANNNNNTGG 1 cut(s) 599
PinAI ACCGGT 1 cut(s) 331
PkrI GCNGC 1 cut(s) 205
PleI GAGTC 1 cut(s) 457
PpsI GAGTC 1 cut(s) 457
PspN4I GGNNCC 1 cut(s) 383
PspPI GGNCC 1 cut(s) 776
PspXI VCTCGAGB 1 cut(s) 423
PsrI GAACNNNNNNTAC 2 cut(s) 619, 651
PsuI RGATCY 1 cut(s) 381
RsaI GTAC 3 cut(s) 125, 702, 907
RsaNI GTAC 3 cut(s) 124, 701, 906
RseI CAYNNNNRTG 4 cut(s) 190, 398, 493, 512
SaqAI TTAA 3 cut(s) 480, 725, 870
SatI GCNGC 1 cut(s) 204
Sau3AI GATC 7 cut(s) 136, 342, 381, 402, 589, 613, 820
Sau96I GGNCC 1 cut(s) 776
SchI GAGTC 1 cut(s) 458
SduI GDGCHC 4 cut(s) 185, 234, 395, 697
SetI ASST 7 cut(s) 25, 255, 276, 430, 652, 686, 792
SfcI CTRYAG 2 cut(s) 258, 651
Sfr274I CTCGAG 1 cut(s) 423
SgrAI CRCCGGYG 1 cut(s) 410
SlaI CTCGAG 1 cut(s) 423
SmiMI CAYNNNNRTG 4 cut(s) 190, 398, 493, 512
SmlI CTYRAG 1 cut(s) 423
SmoI CTYRAG 1 cut(s) 423
Sse9I AATT 6 cut(s) 116, 456, 529, 717, 738, 862
SspMI CTAG 3 cut(s) 80, 239, 630
TaaI ACNGT 1 cut(s) 370
TaiI ACGT 1 cut(s) 276
TaqI TCGA 1 cut(s) 424
TaqII GACCGA 1 cut(s) 523
TasI AATT 6 cut(s) 116, 456, 529, 717, 738, 862
TatI WGTACW 2 cut(s) 123, 700
TfiI GAWTC 4 cut(s) 8, 280, 603, 671
Tru1I TTAA 3 cut(s) 480, 725, 870
Tru9I TTAA 3 cut(s) 480, 725, 870
TscAI CASTG 1 cut(s) 234
TseI GCWGC 1 cut(s) 203
TspDTI ATGAA 2 cut(s) 44, 856
TspGWI ACGGA 1 cut(s) 132
TspRI CASTG 1 cut(s) 234
Van91I CCANNNNNTGG 1 cut(s) 599
XapI RAATTY 1 cut(s) 116
XbaI TCTAGA 1 cut(s) 238
XcmI CCANNNNNNNNNTGG 1 cut(s) 317
XhoI CTCGAG 1 cut(s) 423
XspI CTAG 3 cut(s) 80, 239, 630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.