(R)-mandelonitrile lyase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 110, 351 |
| AcuI | CTGAAG | 1 cut(s) | 326 |
| AfaI | GTAC | 1 cut(s) | 99 |
| AjiI | CACGTC | 1 cut(s) | 438 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 65, 97 |
| AluBI | AGCT | 2 cut(s) | 22, 47 |
| AluI | AGCT | 2 cut(s) | 22, 47 |
| AoxI | GGCC | 1 cut(s) | 172 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 65, 97 |
| AseI | ATTAAT | 1 cut(s) | 12 |
| AspS9I | GGNCC | 2 cut(s) | 81, 173 |
| AsuHPI | GGTGA | 1 cut(s) | 291 |
| AsuII | TTCGAA | 1 cut(s) | 71 |
| AvaII | GGWCC | 1 cut(s) | 81 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 337, 369 |
| BccI | CCATC | 3 cut(s) | 153, 228, 412 |
| BfaI | CTAG | 1 cut(s) | 290 |
| Bme18I | GGWCC | 1 cut(s) | 81 |
| BmgBI | CACGTC | 1 cut(s) | 438 |
| BmgT120I | GGNCC | 2 cut(s) | 81, 173 |
| BmiI | GGNNCC | 2 cut(s) | 83, 143 |
| BmrI | ACTGGG | 1 cut(s) | 200 |
| BmuI | ACTGGG | 1 cut(s) | 200 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 332, 364 |
| Bpu14I | TTCGAA | 1 cut(s) | 71 |
| Bse1I | ACTGG | 2 cut(s) | 129, 206 |
| BseGI | GGATG | 2 cut(s) | 220, 427 |
| BseNI | ACTGG | 2 cut(s) | 129, 206 |
| BshFI | GGCC | 1 cut(s) | 174 |
| BslFI | GGGAC | 2 cut(s) | 67, 201 |
| BsmFI | GGGAC | 2 cut(s) | 67, 201 |
| BsnI | GGCC | 1 cut(s) | 174 |
| Bsp119I | TTCGAA | 1 cut(s) | 71 |
| BspACI | CCGC | 2 cut(s) | 110, 351 |
| BspANI | GGCC | 1 cut(s) | 174 |
| BspLI | GGNNCC | 2 cut(s) | 83, 143 |
| BspT104I | TTCGAA | 1 cut(s) | 71 |
| BsrI | ACTGG | 2 cut(s) | 129, 206 |
| BstBI | TTCGAA | 1 cut(s) | 71 |
| BstDEI | CTNAG | 1 cut(s) | 415 |
| BstF5I | GGATG | 2 cut(s) | 220, 427 |
| BsuRI | GGCC | 1 cut(s) | 174 |
| BtrI | CACGTC | 1 cut(s) | 438 |
| BtsCI | GGATG | 2 cut(s) | 220, 427 |
| BtsIMutI | CAGTG | 1 cut(s) | 136 |
| Cfr13I | GGNCC | 2 cut(s) | 81, 173 |
| Csp6I | GTAC | 1 cut(s) | 98 |
| CviAII | CATG | 1 cut(s) | 58 |
| CviJI | RGCY | 5 cut(s) | 22, 47, 144, 174, 420 |
| CviKI_1 | RGCY | 5 cut(s) | 22, 47, 144, 174, 420 |
| CviQI | GTAC | 1 cut(s) | 98 |
| DdeI | CTNAG | 1 cut(s) | 415 |
| Eco47I | GGWCC | 1 cut(s) | 81 |
| Eco57I | CTGAAG | 1 cut(s) | 326 |
| EcoO109I | RGGNCCY | 1 cut(s) | 81 |
| FaeI | CATG | 1 cut(s) | 61 |
| FaiI | YATR | 6 cut(s) | 59, 63, 226, 242, 246, 338 |
| FaqI | GGGAC | 2 cut(s) | 67, 201 |
| FatI | CATG | 1 cut(s) | 57 |
| FokI | GGATG | 2 cut(s) | 207, 414 |
| FspBI | CTAG | 1 cut(s) | 290 |
| HaeIII | GGCC | 1 cut(s) | 174 |
| Hin1II | CATG | 1 cut(s) | 61 |
| HinfI | GANTC | 3 cut(s) | 54, 68, 179 |
| HphI | GGTGA | 1 cut(s) | 291 |
| Hpy166II | GTNNAC | 2 cut(s) | 6, 299 |
| Hpy188I | TCNGA | 2 cut(s) | 31, 306 |
| Hpy8I | GTNNAC | 2 cut(s) | 6, 299 |
| HpyCH4IV | ACGT | 1 cut(s) | 437 |
| HpyCH4V | TGCA | 2 cut(s) | 92, 134 |
| HpyF3I | CTNAG | 1 cut(s) | 415 |
| HpySE526I | ACGT | 1 cut(s) | 437 |
| Hsp92II | CATG | 1 cut(s) | 61 |
| LmnI | GCTCC | 1 cut(s) | 141 |
| LpnPI | CCDG | 3 cut(s) | 142, 189, 219 |
| MaeI | CTAG | 1 cut(s) | 290 |
| MaeII | ACGT | 1 cut(s) | 437 |
| MaeIII | GTNAC | 1 cut(s) | 257 |
| MboII | GAAGA | 1 cut(s) | 319 |
| MfeI | CAATTG | 1 cut(s) | 135 |
| MluCI | AATT | 3 cut(s) | 13, 135, 264 |
| MnlI | CCTC | 4 cut(s) | 72, 106, 264, 312 |
| MseI | TTAA | 6 cut(s) | 12, 18, 162, 198, 263, 267 |
| MunI | CAATTG | 1 cut(s) | 135 |
| NlaIII | CATG | 1 cut(s) | 61 |
| NlaIV | GGNNCC | 2 cut(s) | 83, 143 |
| NspV | TTCGAA | 1 cut(s) | 71 |
| PacI | TTAATTAA | 1 cut(s) | 267 |
| PfeI | GAWTC | 3 cut(s) | 54, 68, 179 |
| PpuMI | RGGWCCY | 1 cut(s) | 81 |
| PshBI | ATTAAT | 1 cut(s) | 12 |
| Psp5II | RGGWCCY | 1 cut(s) | 81 |
| PspN4I | GGNNCC | 2 cut(s) | 83, 143 |
| PspPI | GGNCC | 2 cut(s) | 81, 173 |
| PspPPI | RGGWCCY | 1 cut(s) | 81 |
| RsaI | GTAC | 1 cut(s) | 99 |
| RsaNI | GTAC | 1 cut(s) | 98 |
| SaqAI | TTAA | 6 cut(s) | 12, 18, 162, 198, 263, 267 |
| Sau96I | GGNCC | 2 cut(s) | 81, 173 |
| SetI | ASST | 9 cut(s) | 24, 40, 49, 83, 117, 304, 319, 400, 440 |
| SfuI | TTCGAA | 1 cut(s) | 71 |
| SgeI | CNNG | 8 cut(s) | 70, 88, 141, 188, 218, 267, 302, 336 |
| SinI | GGWCC | 1 cut(s) | 81 |
| Sse9I | AATT | 3 cut(s) | 13, 135, 264 |
| SsiI | CCGC | 2 cut(s) | 110, 351 |
| SspMI | CTAG | 1 cut(s) | 290 |
| TaiI | ACGT | 1 cut(s) | 440 |
| TaqI | TCGA | 1 cut(s) | 71 |
| TasI | AATT | 3 cut(s) | 13, 135, 264 |
| TfiI | GAWTC | 3 cut(s) | 54, 68, 179 |
| Tru1I | TTAA | 6 cut(s) | 12, 18, 162, 198, 263, 267 |
| Tru9I | TTAA | 6 cut(s) | 12, 18, 162, 198, 263, 267 |
| TscAI | CASTG | 1 cut(s) | 136 |
| TspRI | CASTG | 1 cut(s) | 136 |
| VpaK11BI | GGWCC | 1 cut(s) | 81 |
| VspI | ATTAAT | 1 cut(s) | 12 |
| XspI | CTAG | 1 cut(s) | 290 |