(R)-mandelonitrile lyase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1196 |
| Acc36I | ACCTGC | 1 cut(s) | 1659 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 656 |
| AccII | CGCG | 1 cut(s) | 729 |
| AciI | CCGC | 6 cut(s) | 696, 729, 954, 1306, 1536, 1573 |
| AclWI | GGATC | 2 cut(s) | 678, 1175 |
| AcoI | YGGCCR | 1 cut(s) | 132 |
| AcsI | RAATTY | 4 cut(s) | 497, 780, 1204, 1493 |
| AcuI | CTGAAG | 2 cut(s) | 432, 1283 |
| AfaI | GTAC | 5 cut(s) | 25, 96, 888, 1258, 1673 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 202, 656, 867, 1019 |
| AluBI | AGCT | 7 cut(s) | 165, 279, 395, 734, 1267, 1415, 1433 |
| AluI | AGCT | 7 cut(s) | 165, 279, 395, 734, 1267, 1415, 1433 |
| Alw21I | GWGCWC | 3 cut(s) | 70, 313, 1427 |
| Alw26I | GTCTC | 3 cut(s) | 580, 718, 876 |
| AlwI | GGATC | 2 cut(s) | 678, 1175 |
| Ama87I | CYCGRG | 1 cut(s) | 837 |
| AoxI | GGCC | 3 cut(s) | 132, 961, 1131 |
| ApeKI | GCWGC | 2 cut(s) | 260, 1690 |
| ApoI | RAATTY | 4 cut(s) | 497, 780, 1204, 1493 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 348 |
| AspS9I | GGNCC | 7 cut(s) | 647, 962, 1132, 1241, 1295, 1333, 1448 |
| AsuC2I | CCSGG | 1 cut(s) | 1632 |
| AsuHPI | GGTGA | 2 cut(s) | 185, 995 |
| AvaI | CYCGRG | 1 cut(s) | 837 |
| AvaII | GGWCC | 5 cut(s) | 647, 1241, 1295, 1333, 1448 |
| BalI | TGGCCA | 1 cut(s) | 134 |
| Bbv12I | GWGCWC | 3 cut(s) | 70, 313, 1427 |
| BbvI | GCAGC | 2 cut(s) | 272, 1677 |
| BccI | CCATC | 3 cut(s) | 371, 410, 1235 |
| BceAI | ACGGC | 1 cut(s) | 1549 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 264, 298, 427, 461 |
| BclI | TGATCA | 2 cut(s) | 151, 462 |
| BcnI | CCSGG | 1 cut(s) | 1632 |
| BcoDI | GTCTC | 3 cut(s) | 580, 718, 876 |
| BfaI | CTAG | 1 cut(s) | 1140 |
| BfmI | CTRYAG | 2 cut(s) | 1079, 1165 |
| BfuAI | ACCTGC | 1 cut(s) | 1659 |
| BglII | AGATCT | 1 cut(s) | 1700 |
| BisI | GCNGC | 2 cut(s) | 261, 1691 |
| BlsI | GCNGC | 2 cut(s) | 262, 1692 |
| Bme1390I | CCNGG | 1 cut(s) | 1632 |
| Bme18I | GGWCC | 5 cut(s) | 647, 1241, 1295, 1333, 1448 |
| BmeT110I | CYCGRG | 1 cut(s) | 837 |
| BmgT120I | GGNCC | 7 cut(s) | 647, 962, 1132, 1241, 1295, 1333, 1448 |
| BmrFI | CCNGG | 1 cut(s) | 1632 |
| BmrI | ACTGGG | 2 cut(s) | 565, 989 |
| BmsI | GCATC | 8 cut(s) | 149, 175, 1468, 1490, 1529, 1579, 1619, 1677 |
| BmuI | ACTGGG | 2 cut(s) | 565, 989 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1246 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1121, 1153 |
| BpuEI | CTTGAG | 2 cut(s) | 439, 1629 |
| BpuMI | CCSGG | 1 cut(s) | 1632 |
| Bsa29I | ATCGAT | 1 cut(s) | 1587 |
| BsaAI | YACGTR | 1 cut(s) | 890 |
| BsaI | GGTCTC | 1 cut(s) | 876 |
| BsaJI | CCNNGG | 4 cut(s) | 30, 1329, 1531, 1631 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 202, 656, 867, 1019 |
| Bse1I | ACTGG | 3 cut(s) | 560, 995, 1024 |
| Bse3DI | GCAATG | 1 cut(s) | 1221 |
| BseCI | ATCGAT | 1 cut(s) | 1587 |
| BseDI | CCNNGG | 4 cut(s) | 30, 1329, 1531, 1631 |
| BseGI | GGATG | 5 cut(s) | 190, 322, 1324, 1544, 1610 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 202, 656, 867, 1019 |
| BseMI | GCAATG | 1 cut(s) | 1221 |
| BseMII | CTCAG | 3 cut(s) | 344, 902, 950 |
| BseNI | ACTGG | 3 cut(s) | 560, 995, 1024 |
| BseXI | GCAGC | 2 cut(s) | 272, 1677 |
| BsgI | GTGCAG | 2 cut(s) | 129, 936 |
| Bsh1236I | CGCG | 1 cut(s) | 729 |
| BshFI | GGCC | 3 cut(s) | 134, 963, 1133 |
| BshVI | ATCGAT | 1 cut(s) | 1587 |
| BsiHKAI | GWGCWC | 3 cut(s) | 70, 313, 1427 |
| BsiHKCI | CYCGRG | 1 cut(s) | 837 |
| BsiSI | CCGG | 1 cut(s) | 1631 |
| BslFI | GGGAC | 2 cut(s) | 539, 1648 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 202, 656, 867, 1019 |
| BsmAI | GTCTC | 3 cut(s) | 580, 718, 876 |
| BsmBI | CGTCTC | 1 cut(s) | 718 |
| BsmFI | GGGAC | 2 cut(s) | 539, 1648 |
| BsnI | GGCC | 3 cut(s) | 134, 963, 1133 |
| Bso31I | GGTCTC | 1 cut(s) | 876 |
| BsoBI | CYCGRG | 1 cut(s) | 837 |
| Bsp1286I | GDGCHC | 3 cut(s) | 70, 313, 1427 |
| Bsp143I | GATC | 5 cut(s) | 151, 462, 670, 1180, 1700 |
| BspACI | CCGC | 6 cut(s) | 696, 729, 954, 1306, 1536, 1573 |
| BspANI | GGCC | 3 cut(s) | 134, 963, 1133 |
| BspCNI | CTCAG | 3 cut(s) | 345, 903, 949 |
| BspDI | ATCGAT | 1 cut(s) | 1587 |
| BspFNI | CGCG | 1 cut(s) | 729 |
| BspHI | TCATGA | 1 cut(s) | 154 |
| BspMI | ACCTGC | 1 cut(s) | 1659 |
| BspPI | GGATC | 2 cut(s) | 678, 1175 |
| BspTNI | GGTCTC | 1 cut(s) | 876 |
| BsrDI | GCAATG | 1 cut(s) | 1221 |
| BsrI | ACTGG | 3 cut(s) | 560, 995, 1024 |
| BssECI | CCNNGG | 4 cut(s) | 30, 1329, 1531, 1631 |
| BssMI | GATC | 5 cut(s) | 151, 462, 670, 1180, 1700 |
| BssT1I | CCWWGG | 2 cut(s) | 30, 1329 |
| Bst4CI | ACNGT | 7 cut(s) | 121, 201, 571, 793, 1169, 1510, 1654 |
| Bst6I | CTCTTC | 1 cut(s) | 817 |
| BstBAI | YACGTR | 1 cut(s) | 890 |
| BstC8I | GCNNGC | 1 cut(s) | 277 |
| BstDEI | CTNAG | 5 cut(s) | 18, 353, 911, 936, 950 |
| BstDSI | CCRYGG | 1 cut(s) | 1531 |
| BstF5I | GGATG | 5 cut(s) | 190, 322, 1324, 1544, 1610 |
| BstFNI | CGCG | 1 cut(s) | 729 |
| BstKTI | GATC | 5 cut(s) | 154, 465, 673, 1183, 1703 |
| BstMAI | GTCTC | 3 cut(s) | 580, 718, 876 |
| BstMBI | GATC | 5 cut(s) | 151, 462, 670, 1180, 1700 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 317, 960 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1246 |
| BstSCI | CCNGG | 1 cut(s) | 1630 |
| BstSFI | CTRYAG | 2 cut(s) | 1079, 1165 |
| BstUI | CGCG | 1 cut(s) | 729 |
| BstV1I | GCAGC | 2 cut(s) | 272, 1677 |
| BstX2I | RGATCY | 2 cut(s) | 1180, 1700 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1234 |
| BstYI | RGATCY | 2 cut(s) | 1180, 1700 |
| Bsu15I | ATCGAT | 1 cut(s) | 1587 |
| BsuRI | GGCC | 3 cut(s) | 134, 963, 1133 |
| BsuTUI | ATCGAT | 1 cut(s) | 1587 |
| BtgI | CCRYGG | 1 cut(s) | 1531 |
| BtsCI | GGATG | 5 cut(s) | 190, 322, 1324, 1544, 1610 |
| BtsIMutI | CAGTG | 4 cut(s) | 553, 567, 1017, 1174 |
| BveI | ACCTGC | 1 cut(s) | 1659 |
| Cac8I | GCNNGC | 1 cut(s) | 277 |
| CciI | TCATGA | 1 cut(s) | 154 |
| Cfr13I | GGNCC | 7 cut(s) | 647, 962, 1132, 1241, 1295, 1333, 1448 |
| ClaI | ATCGAT | 1 cut(s) | 1587 |
| CseI | GACGC | 2 cut(s) | 735, 1616 |
| Csp6I | GTAC | 5 cut(s) | 24, 95, 887, 1257, 1672 |
| CviQI | GTAC | 5 cut(s) | 24, 95, 887, 1257, 1672 |
| DdeI | CTNAG | 5 cut(s) | 18, 353, 911, 936, 950 |
| DpnI | GATC | 5 cut(s) | 153, 464, 672, 1182, 1702 |
| DpnII | GATC | 5 cut(s) | 151, 462, 670, 1180, 1700 |
| EaeI | YGGCCR | 1 cut(s) | 132 |
| Eam1104I | CTCTTC | 1 cut(s) | 817 |
| EarI | CTCTTC | 1 cut(s) | 817 |
| EciI | GGCGGA | 1 cut(s) | 1551 |
| Eco130I | CCWWGG | 2 cut(s) | 30, 1329 |
| Eco31I | GGTCTC | 1 cut(s) | 876 |
| Eco47I | GGWCC | 5 cut(s) | 647, 1241, 1295, 1333, 1448 |
| Eco57I | CTGAAG | 2 cut(s) | 432, 1283 |
| Eco88I | CYCGRG | 1 cut(s) | 837 |
| EcoO109I | RGGNCCY | 2 cut(s) | 1132, 1241 |
| EcoRI | GAATTC | 2 cut(s) | 497, 780 |
| EcoT14I | CCWWGG | 2 cut(s) | 30, 1329 |
| EcoT22I | ATGCAT | 2 cut(s) | 142, 1483 |
| ErhI | CCWWGG | 2 cut(s) | 30, 1329 |
| Esp3I | CGTCTC | 1 cut(s) | 718 |
| FalI | AAGNNNNNCTT | 1 cut(s) | 1690 |
| FaqI | GGGAC | 2 cut(s) | 539, 1648 |
| FauI | CCCGC | 1 cut(s) | 1566 |
| FbaI | TGATCA | 2 cut(s) | 151, 462 |
| Fnu4HI | GCNGC | 2 cut(s) | 261, 1691 |
| FokI | GGATG | 5 cut(s) | 197, 309, 1311, 1551, 1597 |
| Fsp4HI | GCNGC | 2 cut(s) | 261, 1691 |
| FspBI | CTAG | 1 cut(s) | 1140 |
| GluI | GCNGC | 2 cut(s) | 261, 1691 |
| HaeIII | GGCC | 3 cut(s) | 134, 963, 1133 |
| HapII | CCGG | 1 cut(s) | 1631 |
| HgaI | GACGC | 2 cut(s) | 735, 1616 |
| HincII | GTYRAC | 1 cut(s) | 1606 |
| HindII | GTYRAC | 1 cut(s) | 1606 |
| HindIII | AAGCTT | 3 cut(s) | 1265, 1413, 1431 |
| HinfI | GANTC | 8 cut(s) | 5, 660, 821, 857, 931, 940, 1218, 1584 |
| HpaII | CCGG | 1 cut(s) | 1631 |
| HphI | GGTGA | 2 cut(s) | 185, 995 |
| Hpy166II | GTNNAC | 3 cut(s) | 387, 1003, 1606 |
| Hpy188I | TCNGA | 9 cut(s) | 354, 412, 434, 912, 939, 1185, 1263, 1282, 1550 |
| Hpy188III | TCNNGA | 7 cut(s) | 155, 296, 513, 796, 1178, 1215, 1502 |
| Hpy8I | GTNNAC | 3 cut(s) | 387, 1003, 1606 |
| Hpy99I | CGWCG | 1 cut(s) | 1423 |
| HpyAV | CCTTC | 2 cut(s) | 338, 714 |
| HpyCH4III | ACNGT | 7 cut(s) | 121, 201, 571, 793, 1169, 1510, 1654 |
| HpyCH4IV | ACGT | 2 cut(s) | 595, 889 |
| HpyCH4V | TGCA | 9 cut(s) | 140, 146, 320, 367, 772, 917, 1322, 1481, 1693 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 317, 960 |
| HpyF3I | CTNAG | 5 cut(s) | 18, 353, 911, 936, 950 |
| HpySE526I | ACGT | 2 cut(s) | 595, 889 |
| Ksp22I | TGATCA | 2 cut(s) | 151, 462 |
| Kzo9I | GATC | 5 cut(s) | 151, 462, 670, 1180, 1700 |
| LmnI | GCTCC | 2 cut(s) | 316, 731 |
| Lsp1109I | GCAGC | 2 cut(s) | 272, 1677 |
| LweI | GCATC | 8 cut(s) | 149, 175, 1468, 1490, 1529, 1579, 1619, 1677 |
| MaeI | CTAG | 1 cut(s) | 1140 |
| MaeII | ACGT | 2 cut(s) | 595, 889 |
| MaeIII | GTNAC | 2 cut(s) | 591, 1389 |
| MalI | GATC | 5 cut(s) | 153, 464, 672, 1182, 1702 |
| MboI | GATC | 5 cut(s) | 151, 462, 670, 1180, 1700 |
| MfeI | CAATTG | 1 cut(s) | 924 |
| MflI | RGATCY | 2 cut(s) | 1180, 1700 |
| MhlI | GDGCHC | 3 cut(s) | 70, 313, 1427 |
| MlsI | TGGCCA | 1 cut(s) | 134 |
| MluNI | TGGCCA | 1 cut(s) | 134 |
| MlyI | GAGTC | 3 cut(s) | 14, 940, 1212 |
| MmeI | TCCRAC | 5 cut(s) | 183, 612, 1208, 1475, 1528 |
| Mox20I | TGGCCA | 1 cut(s) | 134 |
| Mph1103I | ATGCAT | 2 cut(s) | 142, 1483 |
| MroXI | GAANNNNTTC | 1 cut(s) | 348 |
| MscI | TGGCCA | 1 cut(s) | 134 |
| MslI | CAYNNNNRTG | 3 cut(s) | 327, 1655, 1685 |
| Msp20I | TGGCCA | 1 cut(s) | 134 |
| MspI | CCGG | 1 cut(s) | 1631 |
| MspR9I | CCNGG | 1 cut(s) | 1632 |
| MunI | CAATTG | 1 cut(s) | 924 |
| MvnI | CGCG | 1 cut(s) | 729 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 317, 960 |
| NciI | CCSGG | 1 cut(s) | 1632 |
| NdeII | GATC | 5 cut(s) | 151, 462, 670, 1180, 1700 |
| NmuCI | GTSAC | 2 cut(s) | 591, 1389 |
| NsiI | ATGCAT | 2 cut(s) | 142, 1483 |
| PaeR7I | CTCGAG | 1 cut(s) | 837 |
| PagI | TCATGA | 1 cut(s) | 154 |
| PdmI | GAANNNNTTC | 1 cut(s) | 348 |
| PfeI | GAWTC | 5 cut(s) | 660, 821, 857, 940, 1584 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 656 |
| PkrI | GCNGC | 2 cut(s) | 262, 1692 |
| PleI | GAGTC | 3 cut(s) | 13, 939, 1212 |
| PpsI | GAGTC | 3 cut(s) | 13, 939, 1212 |
| Ppu21I | YACGTR | 1 cut(s) | 890 |
| PpuMI | RGGWCCY | 1 cut(s) | 1241 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1246 |
| PsiI | TTATAA | 1 cut(s) | 1196 |
| Psp5II | RGGWCCY | 1 cut(s) | 1241 |
| PspPI | GGNCC | 7 cut(s) | 647, 962, 1132, 1241, 1295, 1333, 1448 |
| PspPPI | RGGWCCY | 1 cut(s) | 1241 |
| PspXI | VCTCGAGB | 1 cut(s) | 837 |
| PsuI | RGATCY | 2 cut(s) | 1180, 1700 |
| RsaI | GTAC | 5 cut(s) | 25, 96, 888, 1258, 1673 |
| RsaNI | GTAC | 5 cut(s) | 24, 95, 887, 1257, 1672 |
| RseI | CAYNNNNRTG | 3 cut(s) | 327, 1655, 1685 |
| SatI | GCNGC | 2 cut(s) | 261, 1691 |
| Sau3AI | GATC | 5 cut(s) | 151, 462, 670, 1180, 1700 |
| Sau96I | GGNCC | 7 cut(s) | 647, 962, 1132, 1241, 1295, 1333, 1448 |
| SchI | GAGTC | 3 cut(s) | 14, 940, 1212 |
| ScrFI | CCNGG | 1 cut(s) | 1632 |
| SduI | GDGCHC | 3 cut(s) | 70, 313, 1427 |
| SfaNI | GCATC | 8 cut(s) | 149, 175, 1468, 1490, 1529, 1579, 1619, 1677 |
| SfcI | CTRYAG | 2 cut(s) | 1079, 1165 |
| Sfr274I | CTCGAG | 1 cut(s) | 837 |
| SinI | GGWCC | 5 cut(s) | 647, 1241, 1295, 1333, 1448 |
| SlaI | CTCGAG | 1 cut(s) | 837 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 327, 1655, 1685 |
| SmlI | CTYRAG | 3 cut(s) | 454, 837, 1644 |
| SmoI | CTYRAG | 3 cut(s) | 454, 837, 1644 |
| SsiI | CCGC | 6 cut(s) | 696, 729, 954, 1306, 1536, 1573 |
| SspMI | CTAG | 1 cut(s) | 1140 |
| StyD4I | CCNGG | 1 cut(s) | 1630 |
| StyI | CCWWGG | 2 cut(s) | 30, 1329 |
| TaaI | ACNGT | 7 cut(s) | 121, 201, 571, 793, 1169, 1510, 1654 |
| TaiI | ACGT | 2 cut(s) | 598, 892 |
| TaqI | TCGA | 7 cut(s) | 70, 795, 838, 877, 1421, 1560, 1587 |
| TatI | WGTACW | 1 cut(s) | 94 |
| TfiI | GAWTC | 5 cut(s) | 660, 821, 857, 940, 1584 |
| TscAI | CASTG | 4 cut(s) | 560, 574, 1024, 1174 |
| TseFI | GTSAC | 2 cut(s) | 591, 1389 |
| TseI | GCWGC | 2 cut(s) | 260, 1690 |
| Tsp45I | GTSAC | 2 cut(s) | 591, 1389 |
| TspDTI | ATGAA | 7 cut(s) | 113, 117, 185, 773, 1042, 1373, 1478 |
| TspGWI | ACGGA | 2 cut(s) | 957, 1603 |
| TspRI | CASTG | 4 cut(s) | 560, 574, 1024, 1174 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 656 |
| VpaK11BI | GGWCC | 5 cut(s) | 647, 1241, 1295, 1333, 1448 |
| XapI | RAATTY | 4 cut(s) | 497, 780, 1204, 1493 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1016 |
| XhoI | CTCGAG | 1 cut(s) | 837 |
| XmnI | GAANNNNTTC | 1 cut(s) | 348 |
| XspI | CTAG | 1 cut(s) | 1140 |
| Zsp2I | ATGCAT | 2 cut(s) | 142, 1483 |