(R)-mandelonitrile lyase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 1176 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 173 |
| AciI | CCGC | 5 cut(s) | 213, 471, 823, 1053, 1090 |
| AclWI | GGATC | 2 cut(s) | 195, 692 |
| AcsI | RAATTY | 4 cut(s) | 31, 297, 721, 1010 |
| AcuI | CTGAAG | 1 cut(s) | 800 |
| AfaI | GTAC | 3 cut(s) | 405, 775, 1190 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 173, 536 |
| AgsI | TTSAA | 8 cut(s) | 97, 257, 505, 726, 788, 829, 929, 1138 |
| AluBI | AGCT | 8 cut(s) | 22, 251, 457, 784, 892, 932, 950, 1086 |
| AluI | AGCT | 8 cut(s) | 22, 251, 457, 784, 892, 932, 950, 1086 |
| Alw21I | GWGCWC | 1 cut(s) | 944 |
| Alw26I | GTCTC | 3 cut(s) | 114, 235, 393 |
| AlwI | GGATC | 2 cut(s) | 195, 692 |
| Ama87I | CYCGRG | 1 cut(s) | 354 |
| AoxI | GGCC | 3 cut(s) | 478, 648, 1164 |
| ApeKI | GCWGC | 1 cut(s) | 1207 |
| ApoI | RAATTY | 4 cut(s) | 31, 297, 721, 1010 |
| AspS9I | GGNCC | 7 cut(s) | 164, 479, 649, 758, 812, 850, 965 |
| AsuC2I | CCSGG | 1 cut(s) | 1149 |
| AsuHPI | GGTGA | 1 cut(s) | 512 |
| AvaI | CYCGRG | 1 cut(s) | 354 |
| AvaII | GGWCC | 5 cut(s) | 164, 758, 812, 850, 965 |
| AxyI | CCTNAGG | 1 cut(s) | 1161 |
| Bbv12I | GWGCWC | 1 cut(s) | 944 |
| BbvCI | CCTCAGC | 1 cut(s) | 453 |
| BbvI | GCAGC | 1 cut(s) | 1194 |
| BccI | CCATC | 1 cut(s) | 752 |
| BceAI | ACGGC | 1 cut(s) | 1066 |
| BcnI | CCSGG | 1 cut(s) | 1149 |
| BcoDI | GTCTC | 3 cut(s) | 114, 235, 393 |
| BfaI | CTAG | 1 cut(s) | 657 |
| BfmI | CTRYAG | 1 cut(s) | 682 |
| BfuAI | ACCTGC | 1 cut(s) | 1176 |
| BglII | AGATCT | 1 cut(s) | 1217 |
| BisI | GCNGC | 1 cut(s) | 1208 |
| BlsI | GCNGC | 1 cut(s) | 1209 |
| Bme1390I | CCNGG | 1 cut(s) | 1149 |
| Bme18I | GGWCC | 5 cut(s) | 164, 758, 812, 850, 965 |
| BmeT110I | CYCGRG | 1 cut(s) | 354 |
| BmgT120I | GGNCC | 7 cut(s) | 164, 479, 649, 758, 812, 850, 965 |
| BmrFI | CCNGG | 1 cut(s) | 1149 |
| BmrI | ACTGGG | 2 cut(s) | 99, 506 |
| BmsI | GCATC | 6 cut(s) | 985, 1007, 1046, 1096, 1136, 1194 |
| BmuI | ACTGGG | 2 cut(s) | 99, 506 |
| BoxI | GACNNNNGTC | 1 cut(s) | 763 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 638, 670 |
| Bpu10I | CCTNAGC | 1 cut(s) | 453 |
| BpuMI | CCSGG | 1 cut(s) | 1149 |
| Bsa29I | ATCGAT | 1 cut(s) | 1104 |
| BsaAI | YACGTR | 1 cut(s) | 407 |
| BsaI | GGTCTC | 1 cut(s) | 393 |
| BsaJI | CCNNGG | 3 cut(s) | 846, 1048, 1148 |
| BsaWI | WCCGGW | 1 cut(s) | 984 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 173, 536 |
| Bse1I | ACTGG | 3 cut(s) | 94, 512, 541 |
| Bse21I | CCTNAGG | 1 cut(s) | 1161 |
| Bse3DI | GCAATG | 1 cut(s) | 738 |
| BseCI | ATCGAT | 1 cut(s) | 1104 |
| BseDI | CCNNGG | 3 cut(s) | 846, 1048, 1148 |
| BseGI | GGATG | 4 cut(s) | 791, 841, 1061, 1127 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 173, 536 |
| BseMI | GCAATG | 1 cut(s) | 738 |
| BseMII | CTCAG | 4 cut(s) | 419, 467, 497, 1175 |
| BseNI | ACTGG | 3 cut(s) | 94, 512, 541 |
| BseXI | GCAGC | 1 cut(s) | 1194 |
| BsgI | GTGCAG | 1 cut(s) | 453 |
| BshFI | GGCC | 3 cut(s) | 480, 650, 1166 |
| BshVI | ATCGAT | 1 cut(s) | 1104 |
| BsiHKAI | GWGCWC | 1 cut(s) | 944 |
| BsiHKCI | CYCGRG | 1 cut(s) | 354 |
| BsiSI | CCGG | 3 cut(s) | 701, 985, 1148 |
| BslFI | GGGAC | 4 cut(s) | 73, 451, 568, 1165 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 173, 536 |
| BsmAI | GTCTC | 3 cut(s) | 114, 235, 393 |
| BsmBI | CGTCTC | 1 cut(s) | 114 |
| BsmFI | GGGAC | 4 cut(s) | 73, 451, 568, 1165 |
| BsnI | GGCC | 3 cut(s) | 480, 650, 1166 |
| Bso31I | GGTCTC | 1 cut(s) | 393 |
| BsoBI | CYCGRG | 1 cut(s) | 354 |
| Bsp1286I | GDGCHC | 1 cut(s) | 944 |
| Bsp143I | GATC | 3 cut(s) | 187, 697, 1217 |
| BspACI | CCGC | 5 cut(s) | 213, 471, 823, 1053, 1090 |
| BspANI | GGCC | 3 cut(s) | 480, 650, 1166 |
| BspCNI | CTCAG | 4 cut(s) | 420, 466, 496, 1174 |
| BspDI | ATCGAT | 1 cut(s) | 1104 |
| BspMI | ACCTGC | 1 cut(s) | 1176 |
| BspPI | GGATC | 2 cut(s) | 195, 692 |
| BspTNI | GGTCTC | 1 cut(s) | 393 |
| BsrDI | GCAATG | 1 cut(s) | 738 |
| BsrI | ACTGG | 3 cut(s) | 94, 512, 541 |
| BssECI | CCNNGG | 3 cut(s) | 846, 1048, 1148 |
| BssMI | GATC | 3 cut(s) | 187, 697, 1217 |
| BssT1I | CCWWGG | 1 cut(s) | 846 |
| Bst4CI | ACNGT | 5 cut(s) | 105, 310, 610, 686, 1027 |
| Bst6I | CTCTTC | 1 cut(s) | 334 |
| BstBAI | YACGTR | 1 cut(s) | 407 |
| BstC8I | GCNNGC | 2 cut(s) | 662, 1088 |
| BstDEI | CTNAG | 5 cut(s) | 428, 453, 467, 483, 1161 |
| BstDSI | CCRYGG | 1 cut(s) | 1048 |
| BstF5I | GGATG | 4 cut(s) | 791, 841, 1061, 1127 |
| BstKTI | GATC | 3 cut(s) | 190, 700, 1220 |
| BstMAI | GTCTC | 3 cut(s) | 114, 235, 393 |
| BstMBI | GATC | 3 cut(s) | 187, 697, 1217 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 477 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 763 |
| BstSCI | CCNGG | 1 cut(s) | 1147 |
| BstSFI | CTRYAG | 1 cut(s) | 682 |
| BstV1I | GCAGC | 1 cut(s) | 1194 |
| BstX2I | RGATCY | 2 cut(s) | 697, 1217 |
| BstYI | RGATCY | 2 cut(s) | 697, 1217 |
| Bsu15I | ATCGAT | 1 cut(s) | 1104 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1161 |
| BsuRI | GGCC | 3 cut(s) | 480, 650, 1166 |
| BsuTUI | ATCGAT | 1 cut(s) | 1104 |
| BtgI | CCRYGG | 1 cut(s) | 1048 |
| BtsCI | GGATG | 4 cut(s) | 791, 841, 1061, 1127 |
| BtsIMutI | CAGTG | 5 cut(s) | 87, 101, 534, 691, 908 |
| BveI | ACCTGC | 1 cut(s) | 1176 |
| Cac8I | GCNNGC | 2 cut(s) | 662, 1088 |
| Cfr13I | GGNCC | 7 cut(s) | 164, 479, 649, 758, 812, 850, 965 |
| ClaI | ATCGAT | 1 cut(s) | 1104 |
| CseI | GACGC | 1 cut(s) | 1133 |
| Csp6I | GTAC | 3 cut(s) | 404, 774, 1189 |
| CviAII | CATG | 5 cut(s) | 63, 233, 302, 874, 1096 |
| CviQI | GTAC | 3 cut(s) | 404, 774, 1189 |
| DdeI | CTNAG | 5 cut(s) | 428, 453, 467, 483, 1161 |
| DpnI | GATC | 3 cut(s) | 189, 699, 1219 |
| DpnII | GATC | 3 cut(s) | 187, 697, 1217 |
| Eam1104I | CTCTTC | 1 cut(s) | 334 |
| EarI | CTCTTC | 1 cut(s) | 334 |
| EciI | GGCGGA | 1 cut(s) | 1068 |
| Eco130I | CCWWGG | 1 cut(s) | 846 |
| Eco31I | GGTCTC | 1 cut(s) | 393 |
| Eco47I | GGWCC | 5 cut(s) | 164, 758, 812, 850, 965 |
| Eco57I | CTGAAG | 1 cut(s) | 800 |
| Eco81I | CCTNAGG | 1 cut(s) | 1161 |
| Eco88I | CYCGRG | 1 cut(s) | 354 |
| EcoO109I | RGGNCCY | 2 cut(s) | 649, 758 |
| EcoRI | GAATTC | 2 cut(s) | 31, 297 |
| EcoT14I | CCWWGG | 1 cut(s) | 846 |
| EcoT22I | ATGCAT | 1 cut(s) | 1000 |
| ErhI | CCWWGG | 1 cut(s) | 846 |
| Esp3I | CGTCTC | 1 cut(s) | 114 |
| FaeI | CATG | 5 cut(s) | 66, 236, 305, 877, 1099 |
| FalI | AAGNNNNNCTT | 1 cut(s) | 1207 |
| FaqI | GGGAC | 4 cut(s) | 73, 451, 568, 1165 |
| FatI | CATG | 5 cut(s) | 62, 232, 301, 873, 1095 |
| FauI | CCCGC | 1 cut(s) | 1083 |
| Fnu4HI | GCNGC | 1 cut(s) | 1208 |
| FokI | GGATG | 4 cut(s) | 778, 828, 1068, 1114 |
| Fsp4HI | GCNGC | 1 cut(s) | 1208 |
| FspBI | CTAG | 1 cut(s) | 657 |
| GluI | GCNGC | 1 cut(s) | 1208 |
| HaeIII | GGCC | 3 cut(s) | 480, 650, 1166 |
| HapII | CCGG | 3 cut(s) | 701, 985, 1148 |
| HgaI | GACGC | 1 cut(s) | 1133 |
| Hin1II | CATG | 5 cut(s) | 66, 236, 305, 877, 1099 |
| HincII | GTYRAC | 1 cut(s) | 1123 |
| HindII | GTYRAC | 1 cut(s) | 1123 |
| HindIII | AAGCTT | 4 cut(s) | 782, 930, 948, 1084 |
| HinfI | GANTC | 6 cut(s) | 177, 338, 374, 448, 735, 1101 |
| HpaII | CCGG | 3 cut(s) | 701, 985, 1148 |
| HphI | GGTGA | 1 cut(s) | 512 |
| Hpy166II | GTNNAC | 2 cut(s) | 520, 1123 |
| Hpy188I | TCNGA | 4 cut(s) | 429, 780, 799, 1067 |
| Hpy188III | TCNNGA | 4 cut(s) | 47, 313, 695, 1019 |
| Hpy8I | GTNNAC | 2 cut(s) | 520, 1123 |
| Hpy99I | CGWCG | 1 cut(s) | 940 |
| HpyAV | CCTTC | 1 cut(s) | 231 |
| HpyCH4III | ACNGT | 5 cut(s) | 105, 310, 610, 686, 1027 |
| HpyCH4IV | ACGT | 1 cut(s) | 406 |
| HpyCH4V | TGCA | 4 cut(s) | 434, 839, 998, 1210 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 477 |
| HpyF3I | CTNAG | 5 cut(s) | 428, 453, 467, 483, 1161 |
| HpySE526I | ACGT | 1 cut(s) | 406 |
| Hsp92II | CATG | 5 cut(s) | 66, 236, 305, 877, 1099 |
| Kzo9I | GATC | 3 cut(s) | 187, 697, 1217 |
| LmnI | GCTCC | 2 cut(s) | 248, 889 |
| Lsp1109I | GCAGC | 1 cut(s) | 1194 |
| LweI | GCATC | 6 cut(s) | 985, 1007, 1046, 1096, 1136, 1194 |
| MaeI | CTAG | 1 cut(s) | 657 |
| MaeII | ACGT | 1 cut(s) | 406 |
| MaeIII | GTNAC | 1 cut(s) | 906 |
| MalI | GATC | 3 cut(s) | 189, 699, 1219 |
| MboI | GATC | 3 cut(s) | 187, 697, 1217 |
| MboII | GAAGA | 5 cut(s) | 316, 321, 889, 892, 1212 |
| MfeI | CAATTG | 1 cut(s) | 441 |
| MflI | RGATCY | 2 cut(s) | 697, 1217 |
| MhlI | GDGCHC | 1 cut(s) | 944 |
| MlyI | GAGTC | 2 cut(s) | 457, 729 |
| MmeI | TCCRAC | 3 cut(s) | 129, 992, 1045 |
| Mph1103I | ATGCAT | 1 cut(s) | 1000 |
| MseI | TTAA | 9 cut(s) | 41, 53, 195, 264, 573, 896, 921, 1200, 1225 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1172, 1202 |
| MspI | CCGG | 3 cut(s) | 701, 985, 1148 |
| MspR9I | CCNGG | 1 cut(s) | 1149 |
| MunI | CAATTG | 1 cut(s) | 441 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 477 |
| NciI | CCSGG | 1 cut(s) | 1149 |
| NdeII | GATC | 3 cut(s) | 187, 697, 1217 |
| NlaIII | CATG | 5 cut(s) | 66, 236, 305, 877, 1099 |
| NmuCI | GTSAC | 1 cut(s) | 906 |
| NsiI | ATGCAT | 1 cut(s) | 1000 |
| PaeR7I | CTCGAG | 1 cut(s) | 354 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 627 |
| PfeI | GAWTC | 4 cut(s) | 177, 338, 374, 1101 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 173 |
| PkrI | GCNGC | 1 cut(s) | 1209 |
| PleI | GAGTC | 2 cut(s) | 456, 729 |
| PpsI | GAGTC | 2 cut(s) | 456, 729 |
| Ppu21I | YACGTR | 1 cut(s) | 407 |
| PpuMI | RGGWCCY | 1 cut(s) | 758 |
| PshAI | GACNNNNGTC | 1 cut(s) | 763 |
| Psp5II | RGGWCCY | 1 cut(s) | 758 |
| PspPI | GGNCC | 7 cut(s) | 164, 479, 649, 758, 812, 850, 965 |
| PspPPI | RGGWCCY | 1 cut(s) | 758 |
| PspXI | VCTCGAGB | 1 cut(s) | 354 |
| PsuI | RGATCY | 2 cut(s) | 697, 1217 |
| RsaI | GTAC | 3 cut(s) | 405, 775, 1190 |
| RsaNI | GTAC | 3 cut(s) | 404, 774, 1189 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1172, 1202 |
| SaqAI | TTAA | 9 cut(s) | 41, 53, 195, 264, 573, 896, 921, 1200, 1225 |
| SatI | GCNGC | 1 cut(s) | 1208 |
| Sau3AI | GATC | 3 cut(s) | 187, 697, 1217 |
| Sau96I | GGNCC | 7 cut(s) | 164, 479, 649, 758, 812, 850, 965 |
| SchI | GAGTC | 2 cut(s) | 457, 729 |
| ScrFI | CCNGG | 1 cut(s) | 1149 |
| SduI | GDGCHC | 1 cut(s) | 944 |
| SfaNI | GCATC | 6 cut(s) | 985, 1007, 1046, 1096, 1136, 1194 |
| SfcI | CTRYAG | 1 cut(s) | 682 |
| Sfr274I | CTCGAG | 1 cut(s) | 354 |
| SinI | GGWCC | 5 cut(s) | 164, 758, 812, 850, 965 |
| SlaI | CTCGAG | 1 cut(s) | 354 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1172, 1202 |
| SmlI | CTYRAG | 1 cut(s) | 354 |
| SmoI | CTYRAG | 1 cut(s) | 354 |
| SsiI | CCGC | 5 cut(s) | 213, 471, 823, 1053, 1090 |
| SspMI | CTAG | 1 cut(s) | 657 |
| StyD4I | CCNGG | 1 cut(s) | 1147 |
| StyI | CCWWGG | 1 cut(s) | 846 |
| TaaI | ACNGT | 5 cut(s) | 105, 310, 610, 686, 1027 |
| TaiI | ACGT | 1 cut(s) | 409 |
| TaqI | TCGA | 5 cut(s) | 312, 355, 938, 1077, 1104 |
| TfiI | GAWTC | 4 cut(s) | 177, 338, 374, 1101 |
| Tru1I | TTAA | 9 cut(s) | 41, 53, 195, 264, 573, 896, 921, 1200, 1225 |
| Tru9I | TTAA | 9 cut(s) | 41, 53, 195, 264, 573, 896, 921, 1200, 1225 |
| TscAI | CASTG | 5 cut(s) | 94, 108, 541, 691, 915 |
| TseFI | GTSAC | 1 cut(s) | 906 |
| TseI | GCWGC | 1 cut(s) | 1207 |
| Tsp45I | GTSAC | 1 cut(s) | 906 |
| TspDTI | ATGAA | 4 cut(s) | 290, 559, 890, 995 |
| TspGWI | ACGGA | 1 cut(s) | 261 |
| TspRI | CASTG | 5 cut(s) | 94, 108, 541, 691, 915 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 173 |
| VpaK11BI | GGWCC | 5 cut(s) | 164, 758, 812, 850, 965 |
| XapI | RAATTY | 4 cut(s) | 31, 297, 721, 1010 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 533 |
| XhoI | CTCGAG | 1 cut(s) | 354 |
| XspI | CTAG | 1 cut(s) | 657 |
| Zsp2I | ATGCAT | 1 cut(s) | 1000 |