Rh4AG121000

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000533
Physical Location & Seq
Reverse (-)
26069965 .. 26092555
22591 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG121000.1

Sequence Viewer

Length: 1935 bp
ATGAGATTGTTTCCAATTCGGAAGGAACCGGAGGCAAGATCCCAGTTCAAACGCCTACTGAAGAACACCAATTTTGGGGCGGTGGGGAACCACAATTCAGCATGCTTCTTCTCAATAAGTACATATCATTTTATCTACACCCTCCATTTCTTTTGTTCTTTCTTCACTCAGCAACGGCAAGGACGTTTAATTGGCTTCCAAAACATGAAGAAATCTGCAATTGCTGCAGTAATACTCCTTCTGCACCTTTTTGTCATCTCTTATCGAACTGATCAGGTTCATTCTTTGGCAACTCCATCTGAGAATGATTTTAGCTACATGAAGTTTGTGCGCTATGCCACCGATCTACCGCTAGAAGAAGAATACGACTACATTGTAGTTGGGGGAGGAACAGCAGGCTGTCCATTGGCAACAACTTTATCCGAAAAGTATTCAGTATTGGTTCTAGAAAGAGGCAGTGTTCCTAGTGCATATCCAAGTGTCTTGCGTGAAGAAAAGTTTGCGAGTAATGTCATGCAGGAAGATGATGGTAAGATCCCAGCTCAAAGGTTCACCTCAGAAGAAGGTGTTCGTAATGCAAGAGGCAGGATCTTGGGTGGGTCGAGTATGATCAATGCTGGTTTTTACTCCAGAGCCGAAAAGGAGTTCTATCTCACATCTGGAATTGAATGGGATATGGATATGGTTTATGCATATACTTGGGTCGAAAACACCACCGTATTCCGCCCGGTTAATGTGGAAACTTGGCAATCTGTTGTGAAAGAAGCCTTATTGGAGGCTGGTGTTACTCCGGACAATGGATTTAATTTGGAACACATTCCAGGAGCTAAAGTTGGGGGTTCGATTTTTGACAGTAATGGAAGGAGGCATGGAGCTGTTGAACTGCTTAATAGAGCAGAGCCAAACAACTTGCAAGTTGCAGTTCATGCCACAGTAGAGAGGATCATCTTTTCTTCCAATGTGTCAAGTTGCTCTGCCACAGGAATTATATACAGTGATTCTGACGGGAGGTCACACCGGGTATTTGTACGTGGTAAAGGAGAGATTATATTGAGTGCTGGAACGATTGGGAGCCCTCAACTTCTGCTACTCAGTGGTGTTGGTCCTCAATCCTACCTTTCGTCTCTTAAAATCCCGGTCATCTACCCTCAACCCAACGTTGGACAGTTTATGTTCGACAATCCTCGTAATTCCATAAACATTTTGCCCCCATTTCTACTTCAGCCTTCATTTCTACAAATTGCTGGAATTGCAACTGATTTCTACATAGAGGCTCTCTCTTATATCGTGCCAAATAATTCCAGCAATGTGACTTTGGCAAATATTGATTCGAAAGTACCTGGACCCTTATCATATGGTTCTCTCCGGTTGCAATCATCTTCTGACGTGAGAGTCGGGCCGAATGTCAGCTTCAACTACTTTGCACATCCGGCCGACCTTGCTCGCTGCGTTAAGGGACTAAGAAAGGTTGCAGACTTATTAAAGACAAACTCGTTGAAACCATTTAGGTCTCAAAATTCTTCGGGTACGGAAGGTTTTAACTTTTATGGACCATCTCTACCTACGAACCAAGCAGATGATGCTCCCTTTGAAACCTTCTGTCGAACTACAGTTGCCACGTTTTGGCATTACCATGGCGGATGCCTTGTGGGAAAGGTGGTCGATAGCAGTTTGCGAGTCATTGGGATCAATTCATTACGTGTTGTTGATGGTTCCACATTCAATTTCTCACCAGGAACCAATCCCCAGGCCACCGTTATGATGTTAGGCAGAGCTCTGACTGTGTCTCTGTGCTTGCAGCAAGAGGATGAAGAGGTGCTGGTGCTGCATACGGATTTGGTCGAAGTCGTCAGCCGATGGAAGGGGACGATTACAAGATTAAAGTTTGGAACTACAAGATGCATAGATGTCTTTTTACTCTTCTCGGGCATCTAG

Protein Analysis

644

Amino Acids

71.12

Weight (kDa)

7.61

Isoelectric Point (pI)

42.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_N PF00732 122 - 391 2.6e-19 GMC oxidoreductase
GMC_oxred_C PF05199 449 - 590 4.7e-27 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1391
AccB7I CCANNNNNTGG 1 cut(s) 1623
AccIII TCCGGA 1 cut(s) 790
AciI CCGC 4 cut(s) 80, 350, 724, 1638
AclI AACGTT 1 cut(s) 1158
AclWI GGATC 5 cut(s) 33, 529, 596, 950, 1694
AcoI YGGCCR 1 cut(s) 1431
AcsI RAATTY 1 cut(s) 1516
AcuI CTGAAG 2 cut(s) 80, 1205
AfaI GTAC 4 cut(s) 121, 1029, 1338, 1528
AfiI CCNNNNNNNGG 5 cut(s) 75, 797, 1160, 1623, 1861
AflIII ACRYGT 1 cut(s) 1699
AgsI TTSAA 7 cut(s) 49, 668, 881, 1414, 1498, 1592, 1723
AhdI GACNNNNNGTC 1 cut(s) 1009
AjiI CACGTC 1 cut(s) 1387
AjnI CCWGG 4 cut(s) 820, 1339, 1732, 1746
AluBI AGCT 6 cut(s) 315, 542, 827, 875, 1410, 1775
AluI AGCT 6 cut(s) 315, 542, 827, 875, 1410, 1775
Alw21I GWGCWC 1 cut(s) 1777
Alw26I GTCTC 3 cut(s) 1128, 1515, 1791
AlwI GGATC 5 cut(s) 33, 529, 596, 950, 1694
Ama87I CYCGRG 1 cut(s) 1924
Aor13HI TCCGGA 1 cut(s) 790
AoxI GGCC 3 cut(s) 1397, 1431, 1749
ApeKI GCWGC 4 cut(s) 224, 1446, 1798, 1825
ApoI RAATTY 1 cut(s) 1516
Asp700I GAANNNNTTC 2 cut(s) 567, 816
AspLEI GCGC 1 cut(s) 333
AspS9I GGNCC 4 cut(s) 1103, 1343, 1397, 1550
AsuC2I CCSGG 3 cut(s) 728, 1019, 1136
AsuHPI GGTGA 2 cut(s) 544, 1722
AsuII TTCGAA 1 cut(s) 1331
AvaI CYCGRG 1 cut(s) 1924
AvaII GGWCC 3 cut(s) 1103, 1343, 1550
BanII GRGCYC 2 cut(s) 1076, 1777
BarI GAAGNNNNNNTAC 2 cut(s) 222, 254
Bbv12I GWGCWC 1 cut(s) 1777
BbvI GCAGC 4 cut(s) 211, 1433, 1810, 1812
BccI CCATC 5 cut(s) 304, 521, 1561, 1703, 1851
BceAI ACGGC 1 cut(s) 191
BciT130I CCWGG 4 cut(s) 822, 1341, 1734, 1748
BclI TGATCA 2 cut(s) 271, 609
BcnI CCSGG 3 cut(s) 728, 1019, 1136
BcoDI GTCTC 3 cut(s) 1128, 1515, 1791
BfaI CTAG 4 cut(s) 353, 446, 465, 1933
BfmI CTRYAG 2 cut(s) 225, 1608
BisI GCNGC 4 cut(s) 225, 1447, 1799, 1826
BlsI GCNGC 4 cut(s) 226, 1448, 1800, 1827
Bme1390I CCNGG 7 cut(s) 728, 822, 1019, 1136, 1341, 1734, 1748
Bme18I GGWCC 3 cut(s) 1103, 1343, 1550
BmeRI GACNNNNNGTC 1 cut(s) 1009
BmeT110I CYCGRG 1 cut(s) 1924
BmgBI CACGTC 1 cut(s) 1387
BmgT120I GGNCC 4 cut(s) 1103, 1343, 1397, 1550
BmiI GGNNCC 6 cut(s) 27, 89, 1073, 1345, 1714, 1738
BmrFI CCNGG 7 cut(s) 728, 822, 1019, 1136, 1341, 1734, 1748
BmrI ACTGGG 1 cut(s) 37
BmsI GCATC 3 cut(s) 1570, 1631, 1889
BmuI ACTGGG 1 cut(s) 37
BplI GAGNNNNNCTC 2 cut(s) 1262, 1294
BpmI CTGGAG 1 cut(s) 613
Bpu14I TTCGAA 1 cut(s) 1331
BpuMI CCSGG 3 cut(s) 728, 1019, 1136
BsaAI YACGTR 2 cut(s) 1031, 1700
BsaI GGTCTC 1 cut(s) 1515
BsaJI CCNNGG 2 cut(s) 1633, 1746
BsaWI WCCGGW 3 cut(s) 28, 790, 1365
BsaXI ACNNNNNCTCC 2 cut(s) 816, 846
Bsc4I CCNNNNNNNGG 5 cut(s) 75, 797, 1160, 1623, 1861
Bse1I ACTGG 1 cut(s) 43
Bse3DI GCAATG 1 cut(s) 1312
BseAI TCCGGA 1 cut(s) 790
BseBI CCWGG 4 cut(s) 822, 1341, 1734, 1748
BseDI CCNNGG 2 cut(s) 1633, 1746
BseGI GGATG 3 cut(s) 1426, 1646, 1813
BseLI CCNNNNNNNGG 5 cut(s) 75, 797, 1160, 1623, 1861
BseMI GCAATG 1 cut(s) 1312
BseMII CTCAG 4 cut(s) 182, 291, 570, 1105
BseNI ACTGG 1 cut(s) 43
BseX3I CGGCCG 1 cut(s) 1431
BseXI GCAGC 4 cut(s) 211, 1433, 1810, 1812
BseYI CCCAGC 1 cut(s) 538
BsgI GTGCAG 1 cut(s) 227
Bsh1285I CGRYCG 1 cut(s) 1434
BshFI GGCC 3 cut(s) 1399, 1433, 1751
BsiEI CGRYCG 1 cut(s) 1434
BsiHKAI GWGCWC 1 cut(s) 1777
BsiHKCI CYCGRG 1 cut(s) 1924
BsiSI CCGG 7 cut(s) 29, 728, 791, 1018, 1136, 1366, 1430
BslFI GGGAC 2 cut(s) 1470, 1879
BslI CCNNNNNNNGG 5 cut(s) 75, 797, 1160, 1623, 1861
BsmAI GTCTC 3 cut(s) 1128, 1515, 1791
BsmBI CGTCTC 1 cut(s) 1128
BsmFI GGGAC 2 cut(s) 1470, 1879
BsnI GGCC 3 cut(s) 1399, 1433, 1751
Bso31I GGTCTC 1 cut(s) 1515
BsoBI CYCGRG 1 cut(s) 1924
Bsp119I TTCGAA 1 cut(s) 1331
Bsp1286I GDGCHC 2 cut(s) 1076, 1777
Bsp13I TCCGGA 1 cut(s) 790
Bsp143I GATC 8 cut(s) 38, 271, 343, 534, 588, 609, 942, 1686
Bsp19I CCATGG 1 cut(s) 1633
BspACI CCGC 4 cut(s) 80, 350, 724, 1638
BspANI GGCC 3 cut(s) 1399, 1433, 1751
BspCNI CTCAG 4 cut(s) 181, 292, 569, 1104
BspEI TCCGGA 1 cut(s) 790
BspLI GGNNCC 6 cut(s) 27, 89, 1073, 1345, 1714, 1738
BspMAI CTGCAG 1 cut(s) 229
BspPI GGATC 5 cut(s) 33, 529, 596, 950, 1694
BspT104I TTCGAA 1 cut(s) 1331
BspTNI GGTCTC 1 cut(s) 1515
BsrDI GCAATG 1 cut(s) 1312
BsrI ACTGG 1 cut(s) 43
BssECI CCNNGG 2 cut(s) 1633, 1746
BssMI GATC 8 cut(s) 38, 271, 343, 534, 588, 609, 942, 1686
BssT1I CCWWGG 1 cut(s) 1633
Bst2UI CCWGG 4 cut(s) 822, 1341, 1734, 1748
Bst4CI ACNGT 8 cut(s) 718, 854, 934, 995, 1167, 1612, 1756, 1783
Bst6I CTCTTC 2 cut(s) 1806, 1925
BstAPI GCANNNNNTGC 3 cut(s) 224, 926, 1580
BstBAI YACGTR 2 cut(s) 1031, 1700
BstBI TTCGAA 1 cut(s) 1331
BstC8I GCNNGC 4 cut(s) 103, 397, 1444, 1796
BstDEI CTNAG 5 cut(s) 168, 300, 556, 1091, 1460
BstDSI CCRYGG 1 cut(s) 1633
BstF5I GGATG 3 cut(s) 1426, 1646, 1813
BstHHI GCGC 1 cut(s) 333
BstKTI GATC 8 cut(s) 41, 274, 346, 537, 591, 612, 945, 1689
BstMAI GTCTC 3 cut(s) 1128, 1515, 1791
BstMBI GATC 8 cut(s) 38, 271, 343, 534, 588, 609, 942, 1686
BstMCI CGRYCG 1 cut(s) 1434
BstMWI GCNNNNNNNGC 7 cut(s) 224, 926, 1250, 1430, 1439, 1580, 1825
BstNI CCWGG 4 cut(s) 822, 1341, 1734, 1748
BstNSI RCATGY 1 cut(s) 105
BstSCI CCNGG 7 cut(s) 726, 820, 1017, 1134, 1339, 1732, 1746
BstSFI CTRYAG 2 cut(s) 225, 1608
BstV1I GCAGC 4 cut(s) 211, 1433, 1810, 1812
BstX2I RGATCY 3 cut(s) 38, 534, 588
BstYI RGATCY 3 cut(s) 38, 534, 588
BstZI CGGCCG 1 cut(s) 1431
BsuRI GGCC 3 cut(s) 1399, 1433, 1751
BtgI CCRYGG 1 cut(s) 1633
BtrI CACGTC 1 cut(s) 1387
BtsCI GGATG 3 cut(s) 1426, 1646, 1813
BtsI GCAGTG 1 cut(s) 463
BtsIMutI CAGTG 3 cut(s) 463, 1000, 1099
Cac8I GCNNGC 4 cut(s) 103, 397, 1444, 1796
CfoI GCGC 1 cut(s) 333
Cfr13I GGNCC 4 cut(s) 1103, 1343, 1397, 1550
Csp6I GTAC 4 cut(s) 120, 1028, 1337, 1527
CviAII CATG 7 cut(s) 102, 205, 319, 514, 869, 926, 1634
CviQI GTAC 4 cut(s) 120, 1028, 1337, 1527
DdeI CTNAG 5 cut(s) 168, 300, 556, 1091, 1460
DpnI GATC 8 cut(s) 40, 273, 345, 536, 590, 611, 944, 1688
DpnII GATC 8 cut(s) 38, 271, 343, 534, 588, 609, 942, 1686
DrdI GACNNNNNNGTC 1 cut(s) 1391
DriI GACNNNNNGTC 1 cut(s) 1009
DseDI GACNNNNNNGTC 1 cut(s) 1391
EaeI YGGCCR 1 cut(s) 1431
EagI CGGCCG 1 cut(s) 1431
Eam1104I CTCTTC 2 cut(s) 1806, 1925
Eam1105I GACNNNNNGTC 1 cut(s) 1009
EarI CTCTTC 2 cut(s) 1806, 1925
EciI GGCGGA 2 cut(s) 713, 1653
Ecl136II GAGCTC 1 cut(s) 1775
EclXI CGGCCG 1 cut(s) 1431
Eco130I CCWWGG 1 cut(s) 1633
Eco24I GRGCYC 2 cut(s) 1076, 1777
Eco31I GGTCTC 1 cut(s) 1515
Eco47I GGWCC 3 cut(s) 1103, 1343, 1550
Eco52I CGGCCG 1 cut(s) 1431
Eco53kI GAGCTC 1 cut(s) 1775
Eco57I CTGAAG 2 cut(s) 80, 1205
Eco88I CYCGRG 1 cut(s) 1924
EcoICRI GAGCTC 1 cut(s) 1775
EcoRII CCWGG 4 cut(s) 820, 1339, 1732, 1746
EcoT14I CCWWGG 1 cut(s) 1633
EcoT22I ATGCAT 2 cut(s) 694, 1904
EcoT38I GRGCYC 2 cut(s) 1076, 1777
ErhI CCWWGG 1 cut(s) 1633
Esp3I CGTCTC 1 cut(s) 1128
FaeI CATG 7 cut(s) 105, 208, 322, 517, 872, 929, 1637
FaqI GGGAC 2 cut(s) 1470, 1879
FatI CATG 7 cut(s) 101, 204, 318, 513, 868, 925, 1633
FauNDI CATATG 1 cut(s) 1354
FbaI TGATCA 2 cut(s) 271, 609
Fnu4HI GCNGC 4 cut(s) 225, 1447, 1799, 1826
FokI GGATG 3 cut(s) 1413, 1653, 1820
FriOI GRGCYC 2 cut(s) 1076, 1777
Fsp4HI GCNGC 4 cut(s) 225, 1447, 1799, 1826
FspBI CTAG 4 cut(s) 353, 446, 465, 1933
GlaI GCGC 1 cut(s) 332
GluI GCNGC 4 cut(s) 225, 1447, 1799, 1826
GsaI CCCAGC 1 cut(s) 542
GsuI CTGGAG 1 cut(s) 613
HaeIII GGCC 3 cut(s) 1399, 1433, 1751
HapII CCGG 7 cut(s) 29, 728, 791, 1018, 1136, 1366, 1430
HhaI GCGC 1 cut(s) 333
Hin1II CATG 7 cut(s) 105, 208, 322, 517, 872, 929, 1637
Hin6I GCGC 1 cut(s) 331
HinP1I GCGC 1 cut(s) 331
HinfI GANTC 4 cut(s) 998, 1328, 1392, 1677
HpaII CCGG 7 cut(s) 29, 728, 791, 1018, 1136, 1366, 1430
HphI GGTGA 2 cut(s) 544, 1722
Hpy166II GTNNAC 1 cut(s) 552
Hpy188I TCNGA 7 cut(s) 21, 301, 424, 559, 1003, 1384, 1779
Hpy188III TCNNGA 4 cut(s) 446, 630, 660, 791
Hpy8I GTNNAC 1 cut(s) 552
HpyAV CCTTC 8 cut(s) 16, 248, 557, 855, 1236, 1526, 1606, 1855
HpyCH4III ACNGT 8 cut(s) 718, 854, 934, 995, 1167, 1612, 1756, 1783
HpyCH4IV ACGT 6 cut(s) 184, 1030, 1158, 1386, 1619, 1699
HpyF10VI GCNNNNNNNGC 7 cut(s) 224, 926, 1250, 1430, 1439, 1580, 1825
HpyF3I CTNAG 5 cut(s) 168, 300, 556, 1091, 1460
HpySE526I ACGT 6 cut(s) 184, 1030, 1158, 1386, 1619, 1699
Hsp92II CATG 7 cut(s) 105, 208, 322, 517, 872, 929, 1637
HspAI GCGC 1 cut(s) 331
Kpn2I TCCGGA 1 cut(s) 790
Ksp22I TGATCA 2 cut(s) 271, 609
Kzo9I GATC 8 cut(s) 38, 271, 343, 534, 588, 609, 942, 1686
LmnI GCTCC 4 cut(s) 824, 872, 1071, 1588
Lsp1109I GCAGC 4 cut(s) 211, 1433, 1810, 1812
LweI GCATC 3 cut(s) 1570, 1631, 1889
MaeI CTAG 4 cut(s) 353, 446, 465, 1933
MaeII ACGT 6 cut(s) 184, 1030, 1158, 1386, 1619, 1699
MaeIII GTNAC 3 cut(s) 784, 1011, 1309
MalI GATC 8 cut(s) 40, 273, 345, 536, 590, 611, 944, 1688
MboI GATC 8 cut(s) 38, 271, 343, 534, 588, 609, 942, 1686
MfeI CAATTG 1 cut(s) 219
MflI RGATCY 3 cut(s) 38, 534, 588
MhlI GDGCHC 2 cut(s) 1076, 1777
MlyI GAGTC 2 cut(s) 1401, 1686
MmeI TCCRAC 1 cut(s) 1141
Mph1103I ATGCAT 2 cut(s) 694, 1904
MroI TCCGGA 1 cut(s) 790
MroXI GAANNNNTTC 2 cut(s) 567, 816
MseI TTAA 9 cut(s) 188, 732, 804, 888, 1128, 1452, 1481, 1539, 1880
MslI CAYNNNNRTG 2 cut(s) 1632, 1757
MspI CCGG 7 cut(s) 29, 728, 791, 1018, 1136, 1366, 1430
MspR9I CCNGG 7 cut(s) 728, 822, 1019, 1136, 1341, 1734, 1748
MunI CAATTG 1 cut(s) 219
MvaI CCWGG 4 cut(s) 822, 1341, 1734, 1748
MwoI GCNNNNNNNGC 7 cut(s) 224, 926, 1250, 1430, 1439, 1580, 1825
NciI CCSGG 3 cut(s) 728, 1019, 1136
NcoI CCATGG 1 cut(s) 1633
NdeI CATATG 1 cut(s) 1354
NdeII GATC 8 cut(s) 38, 271, 343, 534, 588, 609, 942, 1686
NlaIII CATG 7 cut(s) 105, 208, 322, 517, 872, 929, 1637
NlaIV GGNNCC 6 cut(s) 27, 89, 1073, 1345, 1714, 1738
NmuCI GTSAC 2 cut(s) 1011, 1309
NsiI ATGCAT 2 cut(s) 694, 1904
NspI RCATGY 1 cut(s) 105
NspV TTCGAA 1 cut(s) 1331
PaeI GCATGC 1 cut(s) 105
PcsI WCGNNNNNNNCGW 1 cut(s) 181
PdmI GAANNNNTTC 2 cut(s) 567, 816
PfeI GAWTC 2 cut(s) 998, 1328
PflFI GACNNNGTC 1 cut(s) 1783
PflMI CCANNNNNTGG 1 cut(s) 1623
PfoI TCCNGGA 1 cut(s) 820
PkrI GCNGC 4 cut(s) 226, 1448, 1800, 1827
PleI GAGTC 2 cut(s) 1400, 1685
PpsI GAGTC 2 cut(s) 1400, 1685
Ppu21I YACGTR 2 cut(s) 1031, 1700
Psp124BI GAGCTC 1 cut(s) 1777
Psp1406I AACGTT 1 cut(s) 1158
Psp6I CCWGG 4 cut(s) 820, 1339, 1732, 1746
PspFI CCCAGC 1 cut(s) 538
PspGI CCWGG 4 cut(s) 820, 1339, 1732, 1746
PspN4I GGNNCC 6 cut(s) 27, 89, 1073, 1345, 1714, 1738
PspPI GGNCC 4 cut(s) 1103, 1343, 1397, 1550
PstI CTGCAG 1 cut(s) 229
PsuI RGATCY 3 cut(s) 38, 534, 588
PsyI GACNNNGTC 1 cut(s) 1783
RsaI GTAC 4 cut(s) 121, 1029, 1338, 1528
RsaNI GTAC 4 cut(s) 120, 1028, 1337, 1527
RseI CAYNNNNRTG 2 cut(s) 1632, 1757
SacI GAGCTC 1 cut(s) 1777
SaqAI TTAA 9 cut(s) 188, 732, 804, 888, 1128, 1452, 1481, 1539, 1880
SatI GCNGC 4 cut(s) 225, 1447, 1799, 1826
Sau3AI GATC 8 cut(s) 38, 271, 343, 534, 588, 609, 942, 1686
Sau96I GGNCC 4 cut(s) 1103, 1343, 1397, 1550
SchI GAGTC 2 cut(s) 1401, 1686
ScrFI CCNGG 7 cut(s) 728, 822, 1019, 1136, 1341, 1734, 1748
SduI GDGCHC 2 cut(s) 1076, 1777
SfaNI GCATC 3 cut(s) 1570, 1631, 1889
SfcI CTRYAG 2 cut(s) 225, 1608
SfuI TTCGAA 1 cut(s) 1331
SinI GGWCC 3 cut(s) 1103, 1343, 1550
SmiMI CAYNNNNRTG 2 cut(s) 1632, 1757
SphI GCATGC 1 cut(s) 105
SsiI CCGC 4 cut(s) 80, 350, 724, 1638
SspI AATATT 1 cut(s) 1324
SspMI CTAG 4 cut(s) 353, 446, 465, 1933
SstI GAGCTC 1 cut(s) 1777
StyD4I CCNGG 7 cut(s) 726, 820, 1017, 1134, 1339, 1732, 1746
StyI CCWWGG 1 cut(s) 1633
TaaI ACNGT 8 cut(s) 718, 854, 934, 995, 1167, 1612, 1756, 1783
TaiI ACGT 6 cut(s) 187, 1033, 1161, 1389, 1622, 1702
TaqI TCGA 9 cut(s) 265, 602, 705, 842, 1176, 1331, 1603, 1662, 1842
TatI WGTACW 1 cut(s) 119
TfiI GAWTC 2 cut(s) 998, 1328
Tru1I TTAA 9 cut(s) 188, 732, 804, 888, 1128, 1452, 1481, 1539, 1880
Tru9I TTAA 9 cut(s) 188, 732, 804, 888, 1128, 1452, 1481, 1539, 1880
TscAI CASTG 3 cut(s) 463, 1000, 1099
TseFI GTSAC 2 cut(s) 1011, 1309
TseI GCWGC 4 cut(s) 224, 1446, 1798, 1825
Tsp45I GTSAC 2 cut(s) 1011, 1309
TspDTI ATGAA 7 cut(s) 221, 269, 335, 914, 1218, 1683, 1824
TspGWI ACGGA 2 cut(s) 1544, 1847
TspRI CASTG 3 cut(s) 463, 1000, 1099
Tth111I GACNNNGTC 1 cut(s) 1783
Van91I CCANNNNNTGG 1 cut(s) 1623
VpaK11BI GGWCC 3 cut(s) 1103, 1343, 1550
XapI RAATTY 1 cut(s) 1516
XbaI TCTAGA 1 cut(s) 445
XceI RCATGY 1 cut(s) 105
XmnI GAANNNNTTC 2 cut(s) 567, 816
XspI CTAG 4 cut(s) 353, 446, 465, 1933
Zsp2I ATGCAT 2 cut(s) 694, 1904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.