Prupe.1G093800_v2.0.a1

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000533
Physical Location & Seq
Reverse (-)
7212936 .. 7216720
3785 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G093800.1

Sequence Viewer

Length: 1638 bp
ATGGAGAAGTCAAGAATTGGTGTTGTACTATTTCTTTTGTACCTGTCTGTCTTCTATCCTCAATCTAAGGTTCAATCATTCGCCACTCCATCTGATCATGATTTAAGCTACATGAAATTTGTCTATAATTCCACTGATCTGCCATTAGAAGAAGTGTACGACTACATTGTAGTTGGAGGGGGTACAGCAGGCTGCCCATTAGCAGCAACTTTATCAGCGAAGTACTCGGTGCTCCTGCTAGAAAAGGGCAGTGTTCCAAGTGCATATCCAAGTGTTTTGCATCAAGATGAGATTCTTACAACTCTCATGCTAGAAGACGATGGAAAGACGCCAGCTGAAAGGTTCACATCCGAAGATGGTGTGGCCAATGTAAGAGGCAGGGTTCTTGGTGGGTCAAGCATGCTCAACTTTGGTTTCTACACTAGAGCCGATAAAGATTTTTATGGAAACTCAGGAATTGAATGGGACATGGATTTGGTTAGAAAGGCCTATCAATGGGTTGAAAATACTATCATAACCCCCCCGAATGTGTCACGTTGGCAATCTGTTGTGAAGGAAGGTTTGTTAGAGGCTGGTGTTCGTCCAGACAACGGATATAATTTAAATCACATTCTAGGAACTAAGGTCTCGGGTACTCTTTTCGACAATCAGGGATGGAGACATGGAGCTGTGGAATTGCTTAATCTAGGAAACCCGAAGAACTTGCGAGTTGCAGTTCATGCCACAGTAGAGAGAATCATATTCTCTTCCAACGCACCAAGTTTGTCTGCTAGAGGAATCGTGTATAGTGATTCTAAAGGGAGGTCACATAAGGCCTTCATACGTGGTAAGGGAGAGGTTATATTGAGTGCCGGGGCAATTGGAAGCACTCAACTCCTGCTACTTAGTGGTGTTGGCCCAGAGTCCTACCTTTCATCGATCAAAATCCCAGTTGTTCACCCCGAGCCTTACGTTGGACAGTTTATGAGTGATAATCCTCGTAATTACATCACAATATTGCCTCCATTTCAACTGGAATCCTCTAGTGTACAGATCGTTGGCATCACAAGAGATTACTTCATAGAGACTTTCTCGGGGTTGCCATTTTCTACTCCACCCTTTAGTATTTTTCCTGATCCTTCTTTCTCCGCAAAGATAAATTCAACTTACGGACAAATTTTCTACAAAGATCCAGGACCCTTGTCCTATGGTTCGCTTAGGCTGCAGTCGTCCTATGATGTCAAAGTTGGTCCAAATGTACGCTTCAACTACTTTTCAAATCCGCTGGATCTTGCTCGTTGTGTTAGTGCCACAAGGAAGATTGGTGATTTATTAAGCACAAATTCATTGAAGCCATTTAAGGTTCAAGATTTGCCTGGTACAGATGGTTTCAACTTTTTCGGACCACCATTACCAAAGAACCTTACAGATACCGCATCCGTGGAAACATTTTGTCGAGAGACAGTAGCCACATTTTGGCACTACCATGGGGGATGCCTTGTGGGAAAGGTGGTTGATGGGGATTTACGAGTCAAGGGGATCAATGCCTTACGTGTTGTTGATGGATCCACATTCAAGTTCTCACCAGGGACCAATCCTCAGGCCACTGTTATGATGTTAGGAAGGTATACTGGCGTTAAGATGCTGAAAGAAAGATAA

Protein Analysis

546

Amino Acids

59.83

Weight (kDa)

8.12

Isoelectric Point (pI)

35.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1455
AccI GTMKAC 1 cut(s) 1607
AciI CCGC 3 cut(s) 1128, 1262, 1413
AclWI GGATC 6 cut(s) 1109, 1163, 1275, 1526, 1539, 1552
AcoI YGGCCR 1 cut(s) 363
AcsI RAATTY 4 cut(s) 116, 1138, 1155, 1321
AcyI GRCGYC 1 cut(s) 329
AfaI GTAC 9 cut(s) 27, 41, 158, 184, 224, 634, 1029, 1239, 1360
AfiI CCNNNNNNNGG 4 cut(s) 495, 590, 953, 1455
AflIII ACRYGT 1 cut(s) 1531
AjnI CCWGG 3 cut(s) 1171, 1354, 1564
AluBI AGCT 3 cut(s) 108, 335, 668
AluI AGCT 3 cut(s) 108, 335, 668
Alw21I GWGCWC 1 cut(s) 234
Alw26I GTCTC 4 cut(s) 631, 652, 1058, 1433
AlwI GGATC 6 cut(s) 1109, 1163, 1275, 1526, 1539, 1552
Ama87I CYCGRG 3 cut(s) 628, 941, 1072
AoxI GGCC 5 cut(s) 363, 486, 813, 895, 1581
ApeKI GCWGC 3 cut(s) 192, 203, 1201
ApoI RAATTY 4 cut(s) 116, 1138, 1155, 1321
AspS9I GGNCC 5 cut(s) 896, 1175, 1229, 1382, 1569
AsuC2I CCSGG 1 cut(s) 853
AsuHPI GGTGA 3 cut(s) 929, 1316, 1554
AvaI CYCGRG 3 cut(s) 628, 941, 1072
AvaII GGWCC 4 cut(s) 1175, 1229, 1382, 1569
AxyI CCTNAGG 1 cut(s) 1578
BalI TGGCCA 1 cut(s) 365
BamHI GGATCC 1 cut(s) 1544
BbsI GAAGAC 2 cut(s) 43, 321
Bbv12I GWGCWC 1 cut(s) 234
BbvI GCAGC 3 cut(s) 179, 215, 1188
BccI CCATC 7 cut(s) 97, 314, 350, 648, 1358, 1490, 1535
BcgI CGANNNNNNTGC 2 cut(s) 685, 719
BciT130I CCWGG 3 cut(s) 1173, 1356, 1566
BclI TGATCA 1 cut(s) 94
BcnI CCSGG 1 cut(s) 853
BcoDI GTCTC 4 cut(s) 631, 652, 1058, 1433
BfaI CTAG 7 cut(s) 239, 311, 423, 614, 686, 771, 1023
BfmI CTRYAG 1 cut(s) 1202
BisI GCNGC 3 cut(s) 193, 204, 1202
BlsI GCNGC 3 cut(s) 194, 205, 1203
BmcAI AGTACT 1 cut(s) 224
Bme1390I CCNGG 4 cut(s) 853, 1173, 1356, 1566
Bme18I GGWCC 4 cut(s) 1175, 1229, 1382, 1569
BmeT110I CYCGRG 3 cut(s) 628, 941, 1072
BmgT120I GGNCC 5 cut(s) 896, 1175, 1229, 1382, 1569
BmiI GGNNCC 3 cut(s) 1177, 1546, 1570
BmrFI CCNGG 4 cut(s) 853, 1173, 1356, 1566
BmrI ACTGGG 1 cut(s) 923
BmsI GCATC 5 cut(s) 289, 1050, 1424, 1463, 1611
BmuI ACTGGG 1 cut(s) 923
BoxI GACNNNNGTC 1 cut(s) 1180
BpiI GAAGAC 2 cut(s) 43, 321
BplI GAGNNNNNCTC 2 cut(s) 1055, 1087
Bpu10I CCTNAGC 1 cut(s) 1196
BpuMI CCSGG 1 cut(s) 853
Bsa29I ATCGAT 1 cut(s) 917
BsaAI YACGTR 2 cut(s) 824, 1532
BsaBI GATNNNNATC 1 cut(s) 923
BsaHI GRCGYC 1 cut(s) 329
BsaI GGTCTC 1 cut(s) 631
BsaJI CCNNGG 4 cut(s) 852, 1419, 1465, 1565
Bsc4I CCNNNNNNNGG 4 cut(s) 495, 590, 953, 1455
Bse1I ACTGG 3 cut(s) 929, 1017, 1615
Bse21I CCTNAGG 1 cut(s) 1578
Bse8I GATNNNNATC 1 cut(s) 923
BseBI CCWGG 3 cut(s) 1173, 1356, 1566
BseCI ATCGAT 1 cut(s) 917
BseDI CCNNGG 4 cut(s) 852, 1419, 1465, 1565
BseGI GGATG 4 cut(s) 347, 659, 1415, 1478
BseJI GATNNNNATC 1 cut(s) 923
BseLI CCNNNNNNNGG 4 cut(s) 495, 590, 953, 1455
BseMII CTCAG 2 cut(s) 465, 1592
BseNI ACTGG 3 cut(s) 929, 1017, 1615
BseXI GCAGC 3 cut(s) 179, 215, 1188
BshFI GGCC 5 cut(s) 365, 488, 815, 897, 1583
BshVI ATCGAT 1 cut(s) 917
BsiHKAI GWGCWC 1 cut(s) 234
BsiHKCI CYCGRG 3 cut(s) 628, 941, 1072
BsiSI CCGG 1 cut(s) 852
BslFI GGGAC 2 cut(s) 479, 1582
BslI CCNNNNNNNGG 4 cut(s) 495, 590, 953, 1455
BsmAI GTCTC 4 cut(s) 631, 652, 1058, 1433
BsmFI GGGAC 2 cut(s) 479, 1582
BsnI GGCC 5 cut(s) 365, 488, 815, 897, 1583
Bso31I GGTCTC 1 cut(s) 631
BsoBI CYCGRG 3 cut(s) 628, 941, 1072
Bsp1286I GDGCHC 1 cut(s) 234
Bsp1407I TGTACA 1 cut(s) 1027
Bsp143I GATC 9 cut(s) 94, 136, 918, 1032, 1114, 1168, 1267, 1518, 1544
Bsp19I CCATGG 1 cut(s) 1465
BspACI CCGC 3 cut(s) 1128, 1262, 1413
BspANI GGCC 5 cut(s) 365, 488, 815, 897, 1583
BspCNI CTCAG 2 cut(s) 464, 1591
BspDI ATCGAT 1 cut(s) 917
BspHI TCATGA 1 cut(s) 97
BspLI GGNNCC 3 cut(s) 1177, 1546, 1570
BspMAI CTGCAG 1 cut(s) 1206
BspPI GGATC 6 cut(s) 1109, 1163, 1275, 1526, 1539, 1552
BspTNI GGTCTC 1 cut(s) 631
BsrGI TGTACA 1 cut(s) 1027
BsrI ACTGG 3 cut(s) 929, 1017, 1615
BssECI CCNNGG 4 cut(s) 852, 1419, 1465, 1565
BssMI GATC 9 cut(s) 94, 136, 918, 1032, 1114, 1168, 1267, 1518, 1544
BssNAI GTATAC 1 cut(s) 1608
BssNI GRCGYC 1 cut(s) 329
BssT1I CCWWGG 1 cut(s) 1465
Bst1107I GTATAC 1 cut(s) 1608
Bst2UI CCWGG 3 cut(s) 1173, 1356, 1566
Bst4CI ACNGT 4 cut(s) 727, 960, 1444, 1588
Bst6I CTCTTC 1 cut(s) 751
BstACI GRCGYC 1 cut(s) 329
BstAPI GCANNNNNTGC 1 cut(s) 719
BstAUI TGTACA 1 cut(s) 1027
BstBAI YACGTR 2 cut(s) 824, 1532
BstC8I GCNNGC 3 cut(s) 190, 333, 401
BstDEI CTNAG 6 cut(s) 66, 451, 621, 884, 1196, 1578
BstDSI CCRYGG 2 cut(s) 1419, 1465
BstF5I GGATG 4 cut(s) 347, 659, 1415, 1478
BstKTI GATC 9 cut(s) 97, 139, 921, 1035, 1117, 1171, 1270, 1521, 1547
BstMAI GTCTC 4 cut(s) 631, 652, 1058, 1433
BstMBI GATC 9 cut(s) 94, 136, 918, 1032, 1114, 1168, 1267, 1518, 1544
BstMWI GCNNNNNNNGC 2 cut(s) 719, 1201
BstNI CCWGG 3 cut(s) 1173, 1356, 1566
BstNSI RCATGY 1 cut(s) 403
BstPAI GACNNNNGTC 1 cut(s) 1180
BstSCI CCNGG 4 cut(s) 851, 1171, 1354, 1564
BstSFI CTRYAG 1 cut(s) 1202
BstV1I GCAGC 3 cut(s) 179, 215, 1188
BstV2I GAAGAC 2 cut(s) 43, 321
BstX2I RGATCY 3 cut(s) 1168, 1267, 1544
BstYI RGATCY 3 cut(s) 1168, 1267, 1544
BstZ17I GTATAC 1 cut(s) 1608
Bsu15I ATCGAT 1 cut(s) 917
Bsu36I CCTNAGG 1 cut(s) 1578
BsuRI GGCC 5 cut(s) 365, 488, 815, 897, 1583
BsuTUI ATCGAT 1 cut(s) 917
BtgI CCRYGG 2 cut(s) 1419, 1465
BtsCI GGATG 4 cut(s) 347, 659, 1415, 1478
BtsI GCAGTG 1 cut(s) 256
BtsIMutI CAGTG 3 cut(s) 132, 256, 1584
Cac8I GCNNGC 3 cut(s) 190, 333, 401
CciI TCATGA 1 cut(s) 97
Cfr13I GGNCC 5 cut(s) 896, 1175, 1229, 1382, 1569
ClaI ATCGAT 1 cut(s) 917
CseI GACGC 1 cut(s) 337
Csp6I GTAC 9 cut(s) 26, 40, 157, 183, 223, 633, 1028, 1238, 1359
CviAII CATG 8 cut(s) 98, 112, 307, 400, 469, 662, 719, 1466
CviQI GTAC 9 cut(s) 26, 40, 157, 183, 223, 633, 1028, 1238, 1359
DdeI CTNAG 6 cut(s) 66, 451, 621, 884, 1196, 1578
DpnI GATC 9 cut(s) 96, 138, 920, 1034, 1116, 1170, 1269, 1520, 1546
DpnII GATC 9 cut(s) 94, 136, 918, 1032, 1114, 1168, 1267, 1518, 1544
DraI TTTAAA 1 cut(s) 603
EaeI YGGCCR 1 cut(s) 363
Eam1104I CTCTTC 1 cut(s) 751
EarI CTCTTC 1 cut(s) 751
Eco130I CCWWGG 1 cut(s) 1465
Eco147I AGGCCT 2 cut(s) 488, 815
Eco31I GGTCTC 1 cut(s) 631
Eco47I GGWCC 4 cut(s) 1175, 1229, 1382, 1569
Eco81I CCTNAGG 1 cut(s) 1578
Eco88I CYCGRG 3 cut(s) 628, 941, 1072
EcoO109I RGGNCCY 1 cut(s) 1175
EcoRII CCWGG 3 cut(s) 1171, 1354, 1564
EcoT14I CCWWGG 1 cut(s) 1465
ErhI CCWWGG 1 cut(s) 1465
FaeI CATG 8 cut(s) 101, 115, 310, 403, 472, 665, 722, 1469
FaqI GGGAC 2 cut(s) 479, 1582
FatI CATG 8 cut(s) 97, 111, 306, 399, 468, 661, 718, 1465
FbaI TGATCA 1 cut(s) 94
FblI GTMKAC 1 cut(s) 1607
Fnu4HI GCNGC 3 cut(s) 193, 204, 1202
FokI GGATG 4 cut(s) 334, 666, 1402, 1485
Fsp4HI GCNGC 3 cut(s) 193, 204, 1202
FspBI CTAG 7 cut(s) 239, 311, 423, 614, 686, 771, 1023
GluI GCNGC 3 cut(s) 193, 204, 1202
HaeIII GGCC 5 cut(s) 365, 488, 815, 897, 1583
HapII CCGG 1 cut(s) 852
HgaI GACGC 1 cut(s) 337
Hin1I GRCGYC 1 cut(s) 329
Hin1II CATG 8 cut(s) 101, 115, 310, 403, 472, 665, 722, 1469
HinfI GANTC 7 cut(s) 292, 735, 777, 791, 902, 1016, 1509
HpaII CCGG 1 cut(s) 852
HphI GGTGA 3 cut(s) 929, 1316, 1554
Hpy166II GTNNAC 5 cut(s) 157, 345, 937, 1028, 1608
Hpy188I TCNGA 3 cut(s) 94, 352, 1382
Hpy188III TCNNGA 8 cut(s) 12, 98, 284, 453, 584, 1112, 1346, 1436
Hpy8I GTNNAC 5 cut(s) 157, 345, 937, 1028, 1608
HpyAV CCTTC 5 cut(s) 547, 551, 826, 1128, 1596
HpyCH4III ACNGT 4 cut(s) 727, 960, 1444, 1588
HpyCH4IV ACGT 4 cut(s) 535, 823, 951, 1531
HpyCH4V TGCA 4 cut(s) 263, 280, 713, 1204
HpyF10VI GCNNNNNNNGC 2 cut(s) 719, 1201
HpyF3I CTNAG 6 cut(s) 66, 451, 621, 884, 1196, 1578
HpySE526I ACGT 4 cut(s) 535, 823, 951, 1531
Hsp92I GRCGYC 1 cut(s) 329
Hsp92II CATG 8 cut(s) 101, 115, 310, 403, 472, 665, 722, 1469
Ksp22I TGATCA 1 cut(s) 94
Kzo9I GATC 9 cut(s) 94, 136, 918, 1032, 1114, 1168, 1267, 1518, 1544
LmnI GCTCC 2 cut(s) 237, 665
Lsp1109I GCAGC 3 cut(s) 179, 215, 1188
LweI GCATC 5 cut(s) 289, 1050, 1424, 1463, 1611
MaeI CTAG 7 cut(s) 239, 311, 423, 614, 686, 771, 1023
MaeII ACGT 4 cut(s) 535, 823, 951, 1531
MaeIII GTNAC 2 cut(s) 531, 804
MalI GATC 9 cut(s) 96, 138, 920, 1034, 1116, 1170, 1269, 1520, 1546
MboI GATC 9 cut(s) 94, 136, 918, 1032, 1114, 1168, 1267, 1518, 1544
MboII GAAGA 7 cut(s) 43, 161, 326, 365, 709, 738, 1309
MfeI CAATTG 1 cut(s) 858
MflI RGATCY 3 cut(s) 1168, 1267, 1544
MhlI GDGCHC 1 cut(s) 234
MlsI TGGCCA 1 cut(s) 365
MluNI TGGCCA 1 cut(s) 365
MlyI GAGTC 2 cut(s) 911, 1518
MmeI TCCRAC 3 cut(s) 154, 774, 934
Mox20I TGGCCA 1 cut(s) 365
MscI TGGCCA 1 cut(s) 365
MseI TTAA 6 cut(s) 104, 602, 681, 1313, 1338, 1617
MslI CAYNNNNRTG 3 cut(s) 285, 1464, 1589
Msp20I TGGCCA 1 cut(s) 365
MspA1I CMGCKG 2 cut(s) 335, 1264
MspI CCGG 1 cut(s) 852
MspR9I CCNGG 4 cut(s) 853, 1173, 1356, 1566
MunI CAATTG 1 cut(s) 858
MvaI CCWGG 3 cut(s) 1173, 1356, 1566
MwoI GCNNNNNNNGC 2 cut(s) 719, 1201
NciI CCSGG 1 cut(s) 853
NcoI CCATGG 1 cut(s) 1465
NdeII GATC 9 cut(s) 94, 136, 918, 1032, 1114, 1168, 1267, 1518, 1544
NlaIII CATG 8 cut(s) 101, 115, 310, 403, 472, 665, 722, 1469
NlaIV GGNNCC 3 cut(s) 1177, 1546, 1570
NmuCI GTSAC 2 cut(s) 531, 804
NspI RCATGY 1 cut(s) 403
PaeI GCATGC 1 cut(s) 403
PagI TCATGA 1 cut(s) 97
PceI AGGCCT 2 cut(s) 488, 815
PfeI GAWTC 5 cut(s) 292, 735, 777, 791, 1016
PflMI CCANNNNNTGG 1 cut(s) 1455
PfoI TCCNGGA 1 cut(s) 1171
PkrI GCNGC 3 cut(s) 194, 205, 1203
PleI GAGTC 2 cut(s) 910, 1517
PpsI GAGTC 2 cut(s) 910, 1517
Ppu21I YACGTR 2 cut(s) 824, 1532
PpuMI RGGWCCY 1 cut(s) 1175
PshAI GACNNNNGTC 1 cut(s) 1180
Psp5II RGGWCCY 1 cut(s) 1175
Psp6I CCWGG 3 cut(s) 1171, 1354, 1564
PspGI CCWGG 3 cut(s) 1171, 1354, 1564
PspN4I GGNNCC 3 cut(s) 1177, 1546, 1570
PspPI GGNCC 5 cut(s) 896, 1175, 1229, 1382, 1569
PspPPI RGGWCCY 1 cut(s) 1175
PstI CTGCAG 1 cut(s) 1206
PsuI RGATCY 3 cut(s) 1168, 1267, 1544
PvuII CAGCTG 1 cut(s) 335
RsaI GTAC 9 cut(s) 27, 41, 158, 184, 224, 634, 1029, 1239, 1360
RsaNI GTAC 9 cut(s) 26, 40, 157, 183, 223, 633, 1028, 1238, 1359
RseI CAYNNNNRTG 3 cut(s) 285, 1464, 1589
SaqAI TTAA 6 cut(s) 104, 602, 681, 1313, 1338, 1617
SatI GCNGC 3 cut(s) 193, 204, 1202
Sau3AI GATC 9 cut(s) 94, 136, 918, 1032, 1114, 1168, 1267, 1518, 1544
Sau96I GGNCC 5 cut(s) 896, 1175, 1229, 1382, 1569
ScaI AGTACT 1 cut(s) 224
SchI GAGTC 2 cut(s) 911, 1518
ScrFI CCNGG 4 cut(s) 853, 1173, 1356, 1566
SduI GDGCHC 1 cut(s) 234
SfaNI GCATC 5 cut(s) 289, 1050, 1424, 1463, 1611
SfcI CTRYAG 1 cut(s) 1202
SinI GGWCC 4 cut(s) 1175, 1229, 1382, 1569
SmiI ATTTAAAT 1 cut(s) 603
SmiMI CAYNNNNRTG 3 cut(s) 285, 1464, 1589
SphI GCATGC 1 cut(s) 403
SseBI AGGCCT 2 cut(s) 488, 815
SsiI CCGC 3 cut(s) 1128, 1262, 1413
SspI AATATT 1 cut(s) 996
SspMI CTAG 7 cut(s) 239, 311, 423, 614, 686, 771, 1023
StuI AGGCCT 2 cut(s) 488, 815
StyD4I CCNGG 4 cut(s) 851, 1171, 1354, 1564
StyI CCWWGG 1 cut(s) 1465
SwaI ATTTAAAT 1 cut(s) 603
TaaI ACNGT 4 cut(s) 727, 960, 1444, 1588
TaiI ACGT 4 cut(s) 538, 826, 954, 1534
TaqI TCGA 3 cut(s) 642, 917, 1435
TatI WGTACW 3 cut(s) 25, 222, 1027
TfiI GAWTC 5 cut(s) 292, 735, 777, 791, 1016
Tru1I TTAA 6 cut(s) 104, 602, 681, 1313, 1338, 1617
Tru9I TTAA 6 cut(s) 104, 602, 681, 1313, 1338, 1617
TscAI CASTG 3 cut(s) 139, 256, 1591
TseFI GTSAC 2 cut(s) 531, 804
TseI GCWGC 3 cut(s) 192, 203, 1201
Tsp45I GTSAC 2 cut(s) 531, 804
TspDTI ATGAA 6 cut(s) 128, 707, 808, 903, 1048, 1314
TspGWI ACGGA 3 cut(s) 606, 1164, 1408
TspRI CASTG 3 cut(s) 139, 256, 1591
Van91I CCANNNNNTGG 1 cut(s) 1455
VpaK11BI GGWCC 4 cut(s) 1175, 1229, 1382, 1569
XapI RAATTY 4 cut(s) 116, 1138, 1155, 1321
XceI RCATGY 1 cut(s) 403
XmiI GTMKAC 1 cut(s) 1607
XspI CTAG 7 cut(s) 239, 311, 423, 614, 686, 771, 1023
ZrmI AGTACT 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.