Rw4G009830

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000533
Physical Location & Seq
Reverse (-)
22403807 .. 22406521
2715 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G009830.1

Sequence Viewer

Length: 1635 bp
ATGGAGAAATCGACAATAGGTGCTAGACTACTGCTCTTGAACCTTTTTGTACTTGGCTTTCTTGGTCAATCAACAGTTCATTCATTGGCCAATGCATATGCACCTAATCATGATTTTAGCTACATGAAGATTGTGAAGGATGCCACTGATCTACCGTTGGAAGATAAATATGACTATATTGTAGTTGGGGGAGGAACAGCAGGTTGTCCATTAGCAGCAACATTATCAGCAAGCTACTCGGTGCTTGTTCTTGAAAGGGGTAGTGCTCCTACTGCACATCCAAGTGTCTTGCGAGAAGAAGGGTTTCTGAGTAATCTCATGCAAGAAGATGATGGTAAGATCACACCAGCTCAAAAGTTTAAGTCTGAAGACGGTGTTGCTATTGTCAGAGGAAGGGTTCTTGGTGGCTCAAGCATGATCAATGCTGGTTTCTACTCCAAAGCTGACAGGGAATTCTTTGTTAAATCTGGAATTAAATGGGACATGGATTTGGTTGATAAGGCATATCACTGGGTTGAAAGCACTGTCGTCTCTCGTCCAAATCTGTCACGTTCAAATTGGCAATCTATTGTGAAAGAAGCATTGTTGGAGGCTGGTGTTGGTCCAAACAATGGATTCAGTTTGGATCACATTAAGGGAACTAAAGTTGGCGGTTCTACTTTTGATACACATGGAAGGAGATACGGAGCTAACTTGCGAGTGGGAATTCATGCCACAGTCGAGAAAATCATCTTCTCTTCCAAAGAATCATGTGTTTCGGCTCGAGGGGTGATATATATTGATTCTAATGGGAGGTCTCATCGGGCATTTGTACGTGGTAAGGGAGAGGTTATTCTGAGTGCAGGGGCAATTGGGAGTCCTCAGCTTCTACTTCTTAGCGGTGTTGGTCCTCAGTCCTATCTATCATCTTTGAAAATCCCAGTTGTTCACCCCCAACCTCACACTGGAAATTTTATGTATGACAATCCTCGTAATTTCATTAACATTTTGTCCCCGTTTCCACTACAGCCCTCAACTGTAAAGATTGTCGGCATTACCGACCATTTCTACATAGAGACCCTCTCTAGCATGCCCATTTCTACTACACCCTACAGTGTTTTTCCAGATCCGGCTAAACTTACAAAGATAAATTCAAGTTTAGGACTCATTGCCATCAAATTAGTAGGACCTTTGTCTTATGGTACTTTGAAGCTTCAATCATCATCTGATGTGAGAGTTGGTCCAAATGTCCGCTTCAACTACTTTGCACATCCCTTGGACCTTGCTCGTTGTGTTTCTGGCATGAAGAAGATTGGGGAGTTATTAAATACGAAGTCACTGAAAGCATTTAAGGTTCAAGCTTCGTCGAGCACAAAAGCTTACAATTTTTATGGTCCAACTTTACCTATGAACCAGACAGATGATGCATCTTTTGGAGAATTTTGTCGTGATACAGTATCAACAATTTGGCATTTCCACGGCGGATGCCTTGTCGGAAAGGTGGTCGATAGCAGCTTGCGGGTCATGGGAATCAATGCTTTGCGTGTTGTTGACGCATCCACATTCAATTTCACACCGGGGACAAATCCTCAGGCCACACTTATGATGTTGGGCAGGTACATTGGCATTAAGATGCTGCAACAAAGATCTTCTTAA

Protein Analysis

544

Amino Acids

59.0

Weight (kDa)

9.33

Isoelectric Point (pI)

32.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_N PF00732 57 - 300 6.7e-17 GMC oxidoreductase
GMC_oxred_C PF05199 390 - 531 2e-25 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 191, 1584
AccB7I CCANNNNNTGG 1 cut(s) 611
AciI CCGC 5 cut(s) 651, 879, 1231, 1461, 1498
AclWI GGATC 2 cut(s) 633, 1100
AcoI YGGCCR 1 cut(s) 87
AcsI RAATTY 5 cut(s) 452, 705, 949, 1129, 1418
AcuI CTGAAG 1 cut(s) 387
AfaI GTAC 4 cut(s) 51, 813, 1183, 1598
AfiI CCNNNNNNNGG 2 cut(s) 611, 944
Alw21I GWGCWC 2 cut(s) 268, 1352
Alw26I GTCTC 3 cut(s) 535, 801, 1049
AlwI GGATC 2 cut(s) 633, 1100
Ama87I CYCGRG 1 cut(s) 762
AoxI GGCC 2 cut(s) 87, 1572
ApeKI GCWGC 3 cut(s) 215, 1491, 1615
ApoI RAATTY 5 cut(s) 452, 705, 949, 1129, 1418
Asp700I GAANNNNTTC 1 cut(s) 303
AspS9I GGNCC 6 cut(s) 602, 887, 1166, 1220, 1258, 1373
AsuC2I CCSGG 1 cut(s) 1557
AsuHPI GGTGA 2 cut(s) 781, 920
AvaI CYCGRG 1 cut(s) 762
AvaII GGWCC 6 cut(s) 602, 887, 1166, 1220, 1258, 1373
AxyI CCTNAGG 1 cut(s) 1569
BalI TGGCCA 1 cut(s) 89
BbsI GAAGAC 1 cut(s) 375
Bbv12I GWGCWC 2 cut(s) 268, 1352
BbvCI CCTCAGC 1 cut(s) 861
BbvI GCAGC 3 cut(s) 227, 1503, 1602
BccI CCATC 2 cut(s) 326, 1160
BceAI ACGGC 1 cut(s) 1474
BcgI CGANNNNNNTGC 2 cut(s) 219, 253
BclI TGATCA 1 cut(s) 417
BcnI CCSGG 1 cut(s) 1557
BcoDI GTCTC 3 cut(s) 535, 801, 1049
BfaI CTAG 2 cut(s) 24, 1065
BfmI CTRYAG 2 cut(s) 1004, 1090
BfuAI ACCTGC 2 cut(s) 191, 1584
BglII AGATCT 1 cut(s) 1625
BisI GCNGC 3 cut(s) 216, 1492, 1616
BlsI GCNGC 3 cut(s) 217, 1493, 1617
Bme1390I CCNGG 1 cut(s) 1557
Bme18I GGWCC 6 cut(s) 602, 887, 1166, 1220, 1258, 1373
BmeT110I CYCGRG 1 cut(s) 762
BmgT120I GGNCC 6 cut(s) 602, 887, 1166, 1220, 1258, 1373
BmrFI CCNGG 1 cut(s) 1557
BmrI ACTGGG 2 cut(s) 520, 914
BmsI GCATC 6 cut(s) 130, 1393, 1415, 1454, 1544, 1602
BmuI ACTGGG 2 cut(s) 520, 914
BoxI GACNNNNGTC 1 cut(s) 1171
BpiI GAAGAC 1 cut(s) 375
BplI GAGNNNNNCTC 2 cut(s) 1046, 1078
Bpu10I CCTNAGC 1 cut(s) 861
BpuEI CTTGAG 1 cut(s) 394
BpuMI CCSGG 1 cut(s) 1557
BsaAI YACGTR 1 cut(s) 815
BsaI GGTCTC 2 cut(s) 801, 1049
BsaJI CCNNGG 3 cut(s) 1254, 1456, 1556
Bsc4I CCNNNNNNNGG 2 cut(s) 611, 944
Bse1I ACTGG 3 cut(s) 515, 920, 949
Bse21I CCTNAGG 1 cut(s) 1569
Bse3DI GCAATG 1 cut(s) 1146
BseDI CCNNGG 3 cut(s) 1254, 1456, 1556
BseGI GGATG 5 cut(s) 145, 277, 1249, 1469, 1535
BseLI CCNNNNNNNGG 2 cut(s) 611, 944
BseMI GCAATG 1 cut(s) 1146
BseMII CTCAG 5 cut(s) 299, 827, 875, 905, 1583
BseNI ACTGG 3 cut(s) 515, 920, 949
BseXI GCAGC 3 cut(s) 227, 1503, 1602
BsgI GTGCAG 2 cut(s) 258, 861
BshFI GGCC 2 cut(s) 89, 1574
BsiHKAI GWGCWC 2 cut(s) 268, 1352
BsiHKCI CYCGRG 1 cut(s) 762
BsiSI CCGG 2 cut(s) 1109, 1556
BslFI GGGAC 3 cut(s) 494, 976, 1573
BslI CCNNNNNNNGG 2 cut(s) 611, 944
BsmAI GTCTC 3 cut(s) 535, 801, 1049
BsmBI CGTCTC 1 cut(s) 535
BsmFI GGGAC 3 cut(s) 494, 976, 1573
BsnI GGCC 2 cut(s) 89, 1574
Bso31I GGTCTC 2 cut(s) 801, 1049
BsoBI CYCGRG 1 cut(s) 762
Bsp1286I GDGCHC 2 cut(s) 268, 1352
Bsp143I GATC 6 cut(s) 148, 339, 417, 625, 1105, 1625
BspACI CCGC 5 cut(s) 651, 879, 1231, 1461, 1498
BspANI GGCC 2 cut(s) 89, 1574
BspCNI CTCAG 5 cut(s) 300, 828, 874, 904, 1582
BspHI TCATGA 1 cut(s) 109
BspMI ACCTGC 2 cut(s) 191, 1584
BspPI GGATC 2 cut(s) 633, 1100
BspTNI GGTCTC 2 cut(s) 801, 1049
BsrDI GCAATG 1 cut(s) 1146
BsrI ACTGG 3 cut(s) 515, 920, 949
BssECI CCNNGG 3 cut(s) 1254, 1456, 1556
BssMI GATC 6 cut(s) 148, 339, 417, 625, 1105, 1625
BssT1I CCWWGG 1 cut(s) 1254
Bst4CI ACNGT 8 cut(s) 76, 156, 374, 526, 718, 1018, 1094, 1435
Bst6I CTCTTC 1 cut(s) 742
BstBAI YACGTR 1 cut(s) 815
BstC8I GCNNGC 3 cut(s) 232, 1070, 1496
BstDEI CTNAG 6 cut(s) 308, 836, 861, 875, 891, 1569
BstDSI CCRYGG 1 cut(s) 1456
BstF5I GGATG 5 cut(s) 145, 277, 1249, 1469, 1535
BstKTI GATC 6 cut(s) 151, 342, 420, 628, 1108, 1628
BstMAI GTCTC 3 cut(s) 535, 801, 1049
BstMBI GATC 6 cut(s) 148, 339, 417, 625, 1105, 1625
BstMWI GCNNNNNNNGC 1 cut(s) 272
BstNSI RCATGY 1 cut(s) 1072
BstPAI GACNNNNGTC 1 cut(s) 1171
BstSCI CCNGG 1 cut(s) 1555
BstSFI CTRYAG 2 cut(s) 1004, 1090
BstV1I GCAGC 3 cut(s) 227, 1503, 1602
BstV2I GAAGAC 1 cut(s) 375
BstX2I RGATCY 2 cut(s) 1105, 1625
BstYI RGATCY 2 cut(s) 1105, 1625
Bsu36I CCTNAGG 1 cut(s) 1569
BsuRI GGCC 2 cut(s) 89, 1574
BtgI CCRYGG 1 cut(s) 1456
BtsCI GGATG 5 cut(s) 145, 277, 1249, 1469, 1535
BtsIMutI CAGTG 6 cut(s) 144, 508, 522, 942, 1099, 1316
BveI ACCTGC 2 cut(s) 191, 1584
Cac8I GCNNGC 3 cut(s) 232, 1070, 1496
CciI TCATGA 1 cut(s) 109
Cfr13I GGNCC 6 cut(s) 602, 887, 1166, 1220, 1258, 1373
CseI GACGC 1 cut(s) 1541
Csp6I GTAC 4 cut(s) 50, 812, 1182, 1597
CviQI GTAC 4 cut(s) 50, 812, 1182, 1597
DdeI CTNAG 6 cut(s) 308, 836, 861, 875, 891, 1569
DpnI GATC 6 cut(s) 150, 341, 419, 627, 1107, 1627
DpnII GATC 6 cut(s) 148, 339, 417, 625, 1105, 1625
EaeI YGGCCR 1 cut(s) 87
Eam1104I CTCTTC 1 cut(s) 742
EarI CTCTTC 1 cut(s) 742
EciI GGCGGA 1 cut(s) 1476
Eco130I CCWWGG 1 cut(s) 1254
Eco31I GGTCTC 2 cut(s) 801, 1049
Eco47I GGWCC 6 cut(s) 602, 887, 1166, 1220, 1258, 1373
Eco57I CTGAAG 1 cut(s) 387
Eco81I CCTNAGG 1 cut(s) 1569
Eco88I CYCGRG 1 cut(s) 762
EcoO109I RGGNCCY 1 cut(s) 1166
EcoRI GAATTC 2 cut(s) 452, 705
EcoT14I CCWWGG 1 cut(s) 1254
EcoT22I ATGCAT 2 cut(s) 97, 1408
ErhI CCWWGG 1 cut(s) 1254
Esp3I CGTCTC 1 cut(s) 535
FalI AAGNNNNNCTT 1 cut(s) 1615
FaqI GGGAC 3 cut(s) 494, 976, 1573
FauI CCCGC 1 cut(s) 1491
FauNDI CATATG 1 cut(s) 97
FbaI TGATCA 1 cut(s) 417
Fnu4HI GCNGC 3 cut(s) 216, 1492, 1616
FokI GGATG 5 cut(s) 152, 264, 1236, 1476, 1522
Fsp4HI GCNGC 3 cut(s) 216, 1492, 1616
FspBI CTAG 2 cut(s) 24, 1065
GluI GCNGC 3 cut(s) 216, 1492, 1616
HaeIII GGCC 2 cut(s) 89, 1574
HapII CCGG 2 cut(s) 1109, 1556
HgaI GACGC 1 cut(s) 1541
HincII GTYRAC 1 cut(s) 1531
HindII GTYRAC 1 cut(s) 1531
HindIII AAGCTT 3 cut(s) 1190, 1338, 1356
HinfI GANTC 6 cut(s) 615, 746, 782, 856, 1143, 1509
HpaII CCGG 2 cut(s) 1109, 1556
HphI GGTGA 2 cut(s) 781, 920
Hpy166II GTNNAC 2 cut(s) 928, 1531
Hpy188I TCNGA 6 cut(s) 309, 367, 389, 837, 1207, 1475
Hpy188III TCNNGA 7 cut(s) 37, 110, 251, 468, 721, 1103, 1427
Hpy8I GTNNAC 2 cut(s) 928, 1531
Hpy99I CGWCG 1 cut(s) 1348
HpyAV CCTTC 4 cut(s) 130, 293, 387, 669
HpyCH4III ACNGT 8 cut(s) 76, 156, 374, 526, 718, 1018, 1094, 1435
HpyCH4IV ACGT 2 cut(s) 550, 814
HpyCH4V TGCA 8 cut(s) 95, 101, 275, 322, 842, 1247, 1406, 1618
HpyF10VI GCNNNNNNNGC 1 cut(s) 272
HpyF3I CTNAG 6 cut(s) 308, 836, 861, 875, 891, 1569
HpySE526I ACGT 2 cut(s) 550, 814
Ksp22I TGATCA 1 cut(s) 417
Kzo9I GATC 6 cut(s) 148, 339, 417, 625, 1105, 1625
LmnI GCTCC 2 cut(s) 271, 686
Lsp1109I GCAGC 3 cut(s) 227, 1503, 1602
LweI GCATC 6 cut(s) 130, 1393, 1415, 1454, 1544, 1602
MaeI CTAG 2 cut(s) 24, 1065
MaeII ACGT 2 cut(s) 550, 814
MaeIII GTNAC 2 cut(s) 546, 1314
MalI GATC 6 cut(s) 150, 341, 419, 627, 1107, 1627
MboI GATC 6 cut(s) 148, 339, 417, 625, 1105, 1625
MfeI CAATTG 1 cut(s) 849
MflI RGATCY 2 cut(s) 1105, 1625
MhlI GDGCHC 2 cut(s) 268, 1352
MlsI TGGCCA 1 cut(s) 89
MluNI TGGCCA 1 cut(s) 89
MlyI GAGTC 2 cut(s) 865, 1137
MmeI TCCRAC 4 cut(s) 138, 567, 1400, 1453
Mox20I TGGCCA 1 cut(s) 89
Mph1103I ATGCAT 2 cut(s) 97, 1408
MroXI GAANNNNTTC 1 cut(s) 303
MscI TGGCCA 1 cut(s) 89
MseI TTAA 9 cut(s) 360, 462, 474, 633, 981, 1304, 1329, 1608, 1633
MslI CAYNNNNRTG 3 cut(s) 282, 1580, 1610
Msp20I TGGCCA 1 cut(s) 89
MspI CCGG 2 cut(s) 1109, 1556
MspR9I CCNGG 1 cut(s) 1557
MunI CAATTG 1 cut(s) 849
MwoI GCNNNNNNNGC 1 cut(s) 272
NciI CCSGG 1 cut(s) 1557
NdeI CATATG 1 cut(s) 97
NdeII GATC 6 cut(s) 148, 339, 417, 625, 1105, 1625
NmuCI GTSAC 2 cut(s) 546, 1314
NsiI ATGCAT 2 cut(s) 97, 1408
NspI RCATGY 1 cut(s) 1072
PaeI GCATGC 1 cut(s) 1072
PaeR7I CTCGAG 1 cut(s) 762
PagI TCATGA 1 cut(s) 109
PcsI WCGNNNNNNNCGW 1 cut(s) 1035
PdmI GAANNNNTTC 1 cut(s) 303
PfeI GAWTC 4 cut(s) 615, 746, 782, 1509
PflMI CCANNNNNTGG 1 cut(s) 611
PkrI GCNGC 3 cut(s) 217, 1493, 1617
PleI GAGTC 2 cut(s) 864, 1137
PpsI GAGTC 2 cut(s) 864, 1137
Ppu21I YACGTR 1 cut(s) 815
PpuMI RGGWCCY 1 cut(s) 1166
PshAI GACNNNNGTC 1 cut(s) 1171
Psp5II RGGWCCY 1 cut(s) 1166
PspPI GGNCC 6 cut(s) 602, 887, 1166, 1220, 1258, 1373
PspPPI RGGWCCY 1 cut(s) 1166
PspXI VCTCGAGB 1 cut(s) 762
PsuI RGATCY 2 cut(s) 1105, 1625
RsaI GTAC 4 cut(s) 51, 813, 1183, 1598
RsaNI GTAC 4 cut(s) 50, 812, 1182, 1597
RseI CAYNNNNRTG 3 cut(s) 282, 1580, 1610
SaqAI TTAA 9 cut(s) 360, 462, 474, 633, 981, 1304, 1329, 1608, 1633
SatI GCNGC 3 cut(s) 216, 1492, 1616
Sau3AI GATC 6 cut(s) 148, 339, 417, 625, 1105, 1625
Sau96I GGNCC 6 cut(s) 602, 887, 1166, 1220, 1258, 1373
SchI GAGTC 2 cut(s) 865, 1137
ScrFI CCNGG 1 cut(s) 1557
SduI GDGCHC 2 cut(s) 268, 1352
SfaNI GCATC 6 cut(s) 130, 1393, 1415, 1454, 1544, 1602
SfcI CTRYAG 2 cut(s) 1004, 1090
Sfr274I CTCGAG 1 cut(s) 762
SinI GGWCC 6 cut(s) 602, 887, 1166, 1220, 1258, 1373
SlaI CTCGAG 1 cut(s) 762
SmiMI CAYNNNNRTG 3 cut(s) 282, 1580, 1610
SmlI CTYRAG 2 cut(s) 409, 762
SmoI CTYRAG 2 cut(s) 409, 762
SphI GCATGC 1 cut(s) 1072
SsiI CCGC 5 cut(s) 651, 879, 1231, 1461, 1498
SspMI CTAG 2 cut(s) 24, 1065
StyD4I CCNGG 1 cut(s) 1555
StyI CCWWGG 1 cut(s) 1254
TaaI ACNGT 8 cut(s) 76, 156, 374, 526, 718, 1018, 1094, 1435
TaiI ACGT 2 cut(s) 553, 817
TaqI TCGA 5 cut(s) 11, 720, 763, 1346, 1485
TatI WGTACW 1 cut(s) 49
TfiI GAWTC 4 cut(s) 615, 746, 782, 1509
Tru1I TTAA 9 cut(s) 360, 462, 474, 633, 981, 1304, 1329, 1608, 1633
Tru9I TTAA 9 cut(s) 360, 462, 474, 633, 981, 1304, 1329, 1608, 1633
TscAI CASTG 6 cut(s) 151, 515, 529, 949, 1099, 1323
TseFI GTSAC 2 cut(s) 546, 1314
TseI GCWGC 3 cut(s) 215, 1491, 1615
Tsp45I GTSAC 2 cut(s) 546, 1314
TspDTI ATGAA 7 cut(s) 68, 72, 140, 698, 967, 1298, 1403
TspGWI ACGGA 1 cut(s) 699
TspRI CASTG 6 cut(s) 151, 515, 529, 949, 1099, 1323
Van91I CCANNNNNTGG 1 cut(s) 611
VpaK11BI GGWCC 6 cut(s) 602, 887, 1166, 1220, 1258, 1373
XapI RAATTY 5 cut(s) 452, 705, 949, 1129, 1418
XceI RCATGY 1 cut(s) 1072
XcmI CCANNNNNNNNNTGG 1 cut(s) 941
XhoI CTCGAG 1 cut(s) 762
XmnI GAANNNNTTC 1 cut(s) 303
XspI CTAG 2 cut(s) 24, 1065
Zsp2I ATGCAT 2 cut(s) 97, 1408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.