Rroxscaffold_5G00348400

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000533
Physical Location & Seq
Reverse (-)
20515610 .. 20517895
2286 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00348400.1

Sequence Viewer

Length: 1281 bp
ATGAAGTTTGTGCACAATGCCACCGATCTACCGTTAGAAGAAGAATACGACTACATTGTAGTTGGGGGAGGAACAGCAGGTTGTCCATTGGCAACAACTTTATCCGAAAAGTATTCAGTATTGGTTCTAGAAAGAGGCAGTGTTCCTAGTGCATATCCAAGTGTCTTGCGTGAAGAAAAGTTTACGAGTAATCTCATGCAGGAAGATGATGGTAAGATCCCAGCTCAAAGAGCCGAAAAGGAGTTCTATCTCACATCTGGAATCGAATGGGATATGGATATGGTTTATAAGGCATATACTTGGGTCGAAAACACCATCGTATTCCGCCCGGTTAATGTGGAAACTTGGCAATCTGTTGTGAAAGAAGCCTTATTGGAGGCTGGTGTTACTCCGGACAATGGATTTAATTTGGAACACATTCCAGGAGCTAAAGGTTGCTCTGCCACAGGAATTATATATAGTGATTCTGACGGGCGGTCACATCGGGCATTTGTACGTGGTAAAGGAGAGGTTATATTGAGTGCTGGAACGATTGGAAGCCCTCAACTTCTGCTACTCAGTGGTGTTGGTTCTCAATCCTACCTTTCGTCTCTTAAAATCCCGGTCATCTACCCTCAACCCAACGTTGGACAGTTTATGTTCGACAATCCTCGTAATTTCATTAACATTTTGCCCCCATTTCTACTTGAACCTTCAATCCTACAAATTGCGGGAATTACAACTGATTTCTACATAGAGGCTCTCTCTTATATCGTGCCAAATAATTCCAGCAACGTGACTTTGGCAAATATTGCTTCGAAAGTACCTGGACCCTTATCATATGGTTCTCTCCGGCTGCAATCATCTTCTGACGTGAGAGTCGGGCCGAATGTCAGCTTCAACTACTTTGCACATCCGGCCGACCTTGCTCGCTGCGTTAAGGGACTGAGAAAGGTTGCAGACTTATTAAAGACAAACTCGTTGAAACCATTTAGGTCTCAAAATTCTTCGGGTACGGAAGGTTTTAACTTTTATGGACCATCTTTACCTACGAACCAAACAGATGATGCTCCGTTTGAAACCTTCTGTCGAACTACAGTTGCCACATTTTGGCATTACCATGGCGGATGCCTTGTGGGAAAGGTGGTCGATAGCAGTTTGCGAGTCATTGGGATCAATTCATTACGTGTTGTTGATGGTTCCACATTCAATTTCTCACCGGGGACCAATCCCCAGGCCACCATTATGATGTTAGGCAGGTATGTTGGCCTTAAGATGCTACAAGAAAGAGAAGTCAATTGA

Protein Analysis

426

Amino Acids

46.63

Weight (kDa)

5.63

Isoelectric Point (pI)

42.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_N PF00732 143 - 213 6.6e-07 GMC oxidoreductase
GMC_oxred_C PF05199 271 - 412 7.7e-28 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 288
AasI GACNNNNNNGTC 1 cut(s) 857
Acc36I ACCTGC 2 cut(s) 68, 1227
AccB7I CCANNNNNTGG 1 cut(s) 1089
AccIII TCCGGA 1 cut(s) 391
AciI CCGC 4 cut(s) 325, 475, 710, 1104
AclI AACGTT 1 cut(s) 624
AclWI GGATC 2 cut(s) 211, 1160
AcoI YGGCCR 1 cut(s) 897
AcsI RAATTY 1 cut(s) 982
AfaI GTAC 3 cut(s) 495, 804, 994
AfiI CCNNNNNNNGG 3 cut(s) 398, 626, 1089
AflII CTTAAG 1 cut(s) 1250
AflIII ACRYGT 1 cut(s) 1165
AgsI TTSAA 6 cut(s) 689, 696, 880, 964, 1058, 1189
AhdI GACNNNNNGTC 1 cut(s) 475
AjiI CACGTC 1 cut(s) 853
AjnI CCWGG 3 cut(s) 421, 805, 1212
AloI GAACNNNNNNTCC 2 cut(s) 681, 713
AluBI AGCT 3 cut(s) 224, 428, 876
AluI AGCT 3 cut(s) 224, 428, 876
Alw21I GWGCWC 1 cut(s) 15
Alw26I GTCTC 2 cut(s) 594, 981
Alw44I GTGCAC 1 cut(s) 11
AlwI GGATC 2 cut(s) 211, 1160
Aor13HI TCCGGA 1 cut(s) 391
AoxI GGCC 4 cut(s) 863, 897, 1215, 1246
ApaLI GTGCAC 1 cut(s) 11
ApeKI GCWGC 2 cut(s) 835, 912
ApoI RAATTY 1 cut(s) 982
Asp700I GAANNNNTTC 1 cut(s) 417
AspS9I GGNCC 4 cut(s) 809, 863, 1016, 1203
AsuC2I CCSGG 3 cut(s) 329, 602, 1200
AsuHPI GGTGA 1 cut(s) 1188
AsuII TTCGAA 1 cut(s) 797
AvaII GGWCC 3 cut(s) 809, 1016, 1203
BaeGI GKGCMC 1 cut(s) 15
Bbv12I GWGCWC 1 cut(s) 15
BbvI GCAGC 2 cut(s) 822, 899
BccI CCATC 4 cut(s) 203, 323, 1027, 1169
BciT130I CCWGG 3 cut(s) 423, 807, 1214
BcnI CCSGG 3 cut(s) 329, 602, 1200
BcoDI GTCTC 2 cut(s) 594, 981
BfaI CTAG 2 cut(s) 128, 147
BfmI CTRYAG 1 cut(s) 1074
BfrI CTTAAG 1 cut(s) 1250
BfuAI ACCTGC 2 cut(s) 68, 1227
BisI GCNGC 2 cut(s) 836, 913
BlsI GCNGC 2 cut(s) 837, 914
Bme1390I CCNGG 6 cut(s) 329, 423, 602, 807, 1200, 1214
Bme18I GGWCC 3 cut(s) 809, 1016, 1203
BmeRI GACNNNNNGTC 1 cut(s) 475
BmgBI CACGTC 1 cut(s) 853
BmgT120I GGNCC 4 cut(s) 809, 863, 1016, 1203
BmiI GGNNCC 3 cut(s) 811, 1180, 1204
BmrFI CCNGG 6 cut(s) 329, 423, 602, 807, 1200, 1214
BmsI GCATC 3 cut(s) 1036, 1097, 1245
BplI GAGNNNNNCTC 2 cut(s) 728, 760
Bpu14I TTCGAA 1 cut(s) 797
BpuMI CCSGG 3 cut(s) 329, 602, 1200
BsaAI YACGTR 2 cut(s) 497, 1166
BsaI GGTCTC 1 cut(s) 981
BsaJI CCNNGG 3 cut(s) 1099, 1199, 1212
BsaWI WCCGGW 1 cut(s) 391
Bsc4I CCNNNNNNNGG 3 cut(s) 398, 626, 1089
BseAI TCCGGA 1 cut(s) 391
BseBI CCWGG 3 cut(s) 423, 807, 1214
BseDI CCNNGG 3 cut(s) 1099, 1199, 1212
BseGI GGATG 2 cut(s) 892, 1112
BseLI CCNNNNNNNGG 3 cut(s) 398, 626, 1089
BseMII CTCAG 2 cut(s) 571, 917
BseSI GKGCMC 1 cut(s) 15
BseX3I CGGCCG 1 cut(s) 897
BseXI GCAGC 2 cut(s) 822, 899
BseYI CCCAGC 1 cut(s) 220
Bsh1285I CGRYCG 1 cut(s) 900
BshFI GGCC 4 cut(s) 865, 899, 1217, 1248
BsiEI CGRYCG 1 cut(s) 900
BsiHKAI GWGCWC 1 cut(s) 15
BsiSI CCGG 6 cut(s) 329, 392, 602, 832, 896, 1199
BslFI GGGAC 2 cut(s) 936, 1216
BslI CCNNNNNNNGG 3 cut(s) 398, 626, 1089
BsmAI GTCTC 2 cut(s) 594, 981
BsmBI CGTCTC 1 cut(s) 594
BsmFI GGGAC 2 cut(s) 936, 1216
BsnI GGCC 4 cut(s) 865, 899, 1217, 1248
Bso31I GGTCTC 1 cut(s) 981
Bsp119I TTCGAA 1 cut(s) 797
Bsp1286I GDGCHC 1 cut(s) 15
Bsp13I TCCGGA 1 cut(s) 391
Bsp143I GATC 3 cut(s) 25, 216, 1152
Bsp19I CCATGG 1 cut(s) 1099
BspACI CCGC 4 cut(s) 325, 475, 710, 1104
BspANI GGCC 4 cut(s) 865, 899, 1217, 1248
BspCNI CTCAG 2 cut(s) 570, 918
BspEI TCCGGA 1 cut(s) 391
BspLI GGNNCC 3 cut(s) 811, 1180, 1204
BspMI ACCTGC 2 cut(s) 68, 1227
BspPI GGATC 2 cut(s) 211, 1160
BspT104I TTCGAA 1 cut(s) 797
BspTI CTTAAG 1 cut(s) 1250
BspTNI GGTCTC 1 cut(s) 981
BssECI CCNNGG 3 cut(s) 1099, 1199, 1212
BssMI GATC 3 cut(s) 25, 216, 1152
BssT1I CCWWGG 1 cut(s) 1099
Bst2UI CCWGG 3 cut(s) 423, 807, 1214
Bst4CI ACNGT 3 cut(s) 33, 633, 1078
BstAFI CTTAAG 1 cut(s) 1250
BstAPI GCANNNNNTGC 1 cut(s) 791
BstBAI YACGTR 2 cut(s) 497, 1166
BstBI TTCGAA 1 cut(s) 797
BstC8I GCNNGC 1 cut(s) 910
BstDEI CTNAG 2 cut(s) 557, 926
BstDSI CCRYGG 1 cut(s) 1099
BstF5I GGATG 2 cut(s) 892, 1112
BstKTI GATC 3 cut(s) 28, 219, 1155
BstMAI GTCTC 2 cut(s) 594, 981
BstMBI GATC 3 cut(s) 25, 216, 1152
BstMCI CGRYCG 1 cut(s) 900
BstMWI GCNNNNNNNGC 4 cut(s) 230, 791, 896, 905
BstNI CCWGG 3 cut(s) 423, 807, 1214
BstSCI CCNGG 6 cut(s) 327, 421, 600, 805, 1198, 1212
BstSFI CTRYAG 1 cut(s) 1074
BstSLI GKGCMC 1 cut(s) 15
BstV1I GCAGC 2 cut(s) 822, 899
BstX2I RGATCY 1 cut(s) 216
BstYI RGATCY 1 cut(s) 216
BstZI CGGCCG 1 cut(s) 897
BsuRI GGCC 4 cut(s) 865, 899, 1217, 1248
BtgI CCRYGG 1 cut(s) 1099
BtrI CACGTC 1 cut(s) 853
BtsCI GGATG 2 cut(s) 892, 1112
BtsI GCAGTG 1 cut(s) 145
BtsIMutI CAGTG 2 cut(s) 145, 565
BveI ACCTGC 2 cut(s) 68, 1227
Cac8I GCNNGC 1 cut(s) 910
Cfr13I GGNCC 4 cut(s) 809, 863, 1016, 1203
Csp6I GTAC 3 cut(s) 494, 803, 993
CviAII CATG 2 cut(s) 196, 1100
CviQI GTAC 3 cut(s) 494, 803, 993
DdeI CTNAG 2 cut(s) 557, 926
DpnI GATC 3 cut(s) 27, 218, 1154
DpnII GATC 3 cut(s) 25, 216, 1152
DrdI GACNNNNNNGTC 1 cut(s) 857
DriI GACNNNNNGTC 1 cut(s) 475
DseDI GACNNNNNNGTC 1 cut(s) 857
EaeI YGGCCR 1 cut(s) 897
EagI CGGCCG 1 cut(s) 897
Eam1105I GACNNNNNGTC 1 cut(s) 475
EciI GGCGGA 2 cut(s) 314, 1119
EclXI CGGCCG 1 cut(s) 897
Eco130I CCWWGG 1 cut(s) 1099
Eco31I GGTCTC 1 cut(s) 981
Eco47I GGWCC 3 cut(s) 809, 1016, 1203
Eco52I CGGCCG 1 cut(s) 897
EcoRII CCWGG 3 cut(s) 421, 805, 1212
EcoT14I CCWWGG 1 cut(s) 1099
ErhI CCWWGG 1 cut(s) 1099
Esp3I CGTCTC 1 cut(s) 594
FaeI CATG 2 cut(s) 199, 1103
FaqI GGGAC 2 cut(s) 936, 1216
FatI CATG 2 cut(s) 195, 1099
FauI CCCGC 1 cut(s) 703
FauNDI CATATG 1 cut(s) 820
Fnu4HI GCNGC 2 cut(s) 836, 913
FokI GGATG 2 cut(s) 879, 1119
Fsp4HI GCNGC 2 cut(s) 836, 913
FspBI CTAG 2 cut(s) 128, 147
GluI GCNGC 2 cut(s) 836, 913
GsaI CCCAGC 1 cut(s) 224
HaeIII GGCC 4 cut(s) 865, 899, 1217, 1248
HapII CCGG 6 cut(s) 329, 392, 602, 832, 896, 1199
Hin1II CATG 2 cut(s) 199, 1103
HinfI GANTC 4 cut(s) 261, 464, 858, 1143
HpaII CCGG 6 cut(s) 329, 392, 602, 832, 896, 1199
HphI GGTGA 1 cut(s) 1188
Hpy166II GTNNAC 2 cut(s) 13, 183
Hpy188I TCNGA 3 cut(s) 106, 469, 850
Hpy188III TCNNGA 3 cut(s) 128, 258, 392
Hpy8I GTNNAC 2 cut(s) 13, 183
HpyAV CCTTC 3 cut(s) 702, 992, 1072
HpyCH4III ACNGT 3 cut(s) 33, 633, 1078
HpyCH4IV ACGT 5 cut(s) 496, 624, 774, 852, 1165
HpyCH4V TGCA 6 cut(s) 13, 152, 199, 838, 890, 938
HpyF10VI GCNNNNNNNGC 4 cut(s) 230, 791, 896, 905
HpyF3I CTNAG 2 cut(s) 557, 926
HpySE526I ACGT 5 cut(s) 496, 624, 774, 852, 1165
Hsp92II CATG 2 cut(s) 199, 1103
Kpn2I TCCGGA 1 cut(s) 391
Kzo9I GATC 3 cut(s) 25, 216, 1152
LmnI GCTCC 2 cut(s) 425, 1054
Lsp1109I GCAGC 2 cut(s) 822, 899
LweI GCATC 3 cut(s) 1036, 1097, 1245
MaeI CTAG 2 cut(s) 128, 147
MaeII ACGT 5 cut(s) 496, 624, 774, 852, 1165
MaeIII GTNAC 3 cut(s) 385, 477, 775
MalI GATC 3 cut(s) 27, 218, 1154
MboI GATC 3 cut(s) 25, 216, 1152
MboII GAAGA 6 cut(s) 50, 53, 185, 215, 837, 978
MfeI CAATTG 1 cut(s) 1276
MflI RGATCY 1 cut(s) 216
MhlI GDGCHC 1 cut(s) 15
MlyI GAGTC 2 cut(s) 867, 1152
MmeI TCCRAC 1 cut(s) 607
MnlI CCTC 8 cut(s) 62, 128, 370, 502, 552, 624, 660, 730
MroI TCCGGA 1 cut(s) 391
MroXI GAANNNNTTC 1 cut(s) 417
MseI TTAA 8 cut(s) 333, 405, 594, 663, 918, 947, 1005, 1251
MslI CAYNNNNRTG 3 cut(s) 1098, 1223, 1226
MspCI CTTAAG 1 cut(s) 1250
MspI CCGG 6 cut(s) 329, 392, 602, 832, 896, 1199
MspR9I CCNGG 6 cut(s) 329, 423, 602, 807, 1200, 1214
MunI CAATTG 1 cut(s) 1276
MvaI CCWGG 3 cut(s) 423, 807, 1214
MwoI GCNNNNNNNGC 4 cut(s) 230, 791, 896, 905
NciI CCSGG 3 cut(s) 329, 602, 1200
NcoI CCATGG 1 cut(s) 1099
NdeI CATATG 1 cut(s) 820
NdeII GATC 3 cut(s) 25, 216, 1152
NlaIII CATG 2 cut(s) 199, 1103
NlaIV GGNNCC 3 cut(s) 811, 1180, 1204
NmuCI GTSAC 2 cut(s) 477, 775
NspV TTCGAA 1 cut(s) 797
PdmI GAANNNNTTC 1 cut(s) 417
PfeI GAWTC 2 cut(s) 261, 464
PflMI CCANNNNNTGG 1 cut(s) 1089
PfoI TCCNGGA 1 cut(s) 421
PkrI GCNGC 2 cut(s) 837, 914
PleI GAGTC 2 cut(s) 866, 1151
PpsI GAGTC 2 cut(s) 866, 1151
Ppu21I YACGTR 2 cut(s) 497, 1166
PsiI TTATAA 1 cut(s) 288
Psp1406I AACGTT 1 cut(s) 624
Psp6I CCWGG 3 cut(s) 421, 805, 1212
PspFI CCCAGC 1 cut(s) 220
PspGI CCWGG 3 cut(s) 421, 805, 1212
PspN4I GGNNCC 3 cut(s) 811, 1180, 1204
PspPI GGNCC 4 cut(s) 809, 863, 1016, 1203
PsuI RGATCY 1 cut(s) 216
RsaI GTAC 3 cut(s) 495, 804, 994
RsaNI GTAC 3 cut(s) 494, 803, 993
RseI CAYNNNNRTG 3 cut(s) 1098, 1223, 1226
SaqAI TTAA 8 cut(s) 333, 405, 594, 663, 918, 947, 1005, 1251
SatI GCNGC 2 cut(s) 836, 913
Sau3AI GATC 3 cut(s) 25, 216, 1152
Sau96I GGNCC 4 cut(s) 809, 863, 1016, 1203
SchI GAGTC 2 cut(s) 867, 1152
ScrFI CCNGG 6 cut(s) 329, 423, 602, 807, 1200, 1214
SduI GDGCHC 1 cut(s) 15
SfaNI GCATC 3 cut(s) 1036, 1097, 1245
SfcI CTRYAG 1 cut(s) 1074
SfuI TTCGAA 1 cut(s) 797
SinI GGWCC 3 cut(s) 809, 1016, 1203
SmiMI CAYNNNNRTG 3 cut(s) 1098, 1223, 1226
SmlI CTYRAG 1 cut(s) 1250
SmoI CTYRAG 1 cut(s) 1250
SsiI CCGC 4 cut(s) 325, 475, 710, 1104
SspI AATATT 1 cut(s) 790
SspMI CTAG 2 cut(s) 128, 147
StyD4I CCNGG 6 cut(s) 327, 421, 600, 805, 1198, 1212
StyI CCWWGG 1 cut(s) 1099
TaaI ACNGT 3 cut(s) 33, 633, 1078
TaiI ACGT 5 cut(s) 499, 627, 777, 855, 1168
TaqI TCGA 6 cut(s) 264, 306, 642, 797, 1069, 1128
TfiI GAWTC 2 cut(s) 261, 464
Tru1I TTAA 8 cut(s) 333, 405, 594, 663, 918, 947, 1005, 1251
Tru9I TTAA 8 cut(s) 333, 405, 594, 663, 918, 947, 1005, 1251
TscAI CASTG 2 cut(s) 145, 565
TseFI GTSAC 2 cut(s) 477, 775
TseI GCWGC 2 cut(s) 835, 912
Tsp45I GTSAC 2 cut(s) 477, 775
TspDTI ATGAA 3 cut(s) 17, 649, 1149
TspGWI ACGGA 2 cut(s) 1010, 1041
TspRI CASTG 2 cut(s) 145, 565
Van91I CCANNNNNTGG 1 cut(s) 1089
Vha464I CTTAAG 1 cut(s) 1250
VneI GTGCAC 1 cut(s) 11
VpaK11BI GGWCC 3 cut(s) 809, 1016, 1203
XapI RAATTY 1 cut(s) 982
XbaI TCTAGA 1 cut(s) 127
XmnI GAANNNNTTC 1 cut(s) 417
XspI CTAG 2 cut(s) 128, 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.