MD03G1091400.v1.1

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000533
Physical Location & Seq
Forward (+)
7593515 .. 7594710
1196 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1091400.v1.1.491

Sequence Viewer

Length: 882 bp
ATGCAAGAAGATGACGGTAAGACGCCGGCCCAGAGGTTCACATCAGAAGATGGTGTAGCTAATCTAAGAGGACGGATCCTAGGCGGGTCAAGTATGATCAATATTGGTTTTTACTCAAGAGCCGACGGTGAATTCTACCAGAAATCAGGAATTAAATTGGACATGAACTTAGTCAATAATTCATATGCATGGGTTGAAAACACTGTAGCATTCCGTCCGAATGTAACACACTGGCAGTCTGTTGTGAAAGATGCAATGTTAGAAGCTGGTGTTCGTCCAGACAATGGACTGACTTTGGATCACATTCTGGGAAGTAAAGTCTCGGCTACGCTCTTTGACGATCGTGGAAAAAGACATGGAGCTGTGGAACACCTAAATAAAGCACATCCGAAAAACCTGAGAGTTGCAATTCTTGCCACAGTAGAGAGGATCATTTTCTCTTCCAAAGCATCAGGTTTGTCGGCTAAAGGAATCATATACAGTGATTCTAACGGGAGGTCTCATCGGGCATTGATACGTGGTAAGGGTGAGGTTATATTGAGTGCAGATGCAATTGGAAGTTCTCAACTTCTACTACTTAGTGGTGTTGGCCCAAAATCTTACCTTTCATCTCGTAAAATCCCAGTTGTTCACCCACAACCTTACTTTGGGCAGTTTATGCGTGATAATCCTCGTAATTACATTACCATTGTGCCCCCGTTTCAAGTAGAACCTTCTACTGCACAGGTTGCTGGTATCACAAGTGATTATTACATAGAGACTTTCTCCGGCTTGCCATTTTCTACTCAGGCCTTTAGTCTTTTCCCTAGTCCAACTATTCCCATGATCATAAATTCAAGTTTCGGGCAAATTGCTGTCAAAATTCCAGGACCCTTGTCCTAA

Protein Analysis

294

Amino Acids

31.88

Weight (kDa)

9.44

Isoelectric Point (pI)

36.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_N PF00732 22 - 201 1.3e-15 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 284
AciI CCGC 1 cut(s) 84
AclWI GGATC 4 cut(s) 70, 83, 306, 437
AcsI RAATTY 3 cut(s) 131, 832, 861
AcyI GRCGYC 1 cut(s) 23
AfiI CCNNNNNNNGG 2 cut(s) 284, 647
AgsI TTSAA 3 cut(s) 197, 704, 837
AjnI CCWGG 1 cut(s) 865
AluBI AGCT 3 cut(s) 59, 266, 362
AluI AGCT 3 cut(s) 59, 266, 362
Alw26I GTCTC 3 cut(s) 325, 504, 752
AlwI GGATC 4 cut(s) 70, 83, 306, 437
AoxI GGCC 3 cut(s) 27, 589, 789
ApoI RAATTY 3 cut(s) 131, 832, 861
AspA2I CCTAGG 1 cut(s) 79
AspS9I GGNCC 3 cut(s) 28, 590, 869
AsuHPI GGTGA 3 cut(s) 140, 539, 623
AvaII GGWCC 1 cut(s) 869
AvrII CCTAGG 1 cut(s) 79
BaeGI GKGCMC 1 cut(s) 696
BamHI GGATCC 1 cut(s) 75
BccI CCATC 1 cut(s) 44
BciT130I CCWGG 1 cut(s) 867
BclI TGATCA 2 cut(s) 96, 825
BcoDI GTCTC 3 cut(s) 325, 504, 752
BfaI CTAG 2 cut(s) 80, 807
BfmI CTRYAG 1 cut(s) 204
BlnI CCTAGG 1 cut(s) 79
Bme1390I CCNGG 1 cut(s) 867
Bme18I GGWCC 1 cut(s) 869
BmgT120I GGNCC 3 cut(s) 28, 590, 869
BmiI GGNNCC 2 cut(s) 77, 871
BmrFI CCNGG 1 cut(s) 867
BmrI ACTGGG 1 cut(s) 617
BmsI GCATC 3 cut(s) 241, 458, 538
BmuI ACTGGG 1 cut(s) 617
BoxI GACNNNNGTC 1 cut(s) 874
BplI GAGNNNNNCTC 2 cut(s) 749, 781
BpuEI CTTGAG 1 cut(s) 100
BsaAI YACGTR 1 cut(s) 518
BsaHI GRCGYC 1 cut(s) 23
BsaI GGTCTC 1 cut(s) 504
BsaJI CCNNGG 1 cut(s) 79
Bsc4I CCNNNNNNNGG 2 cut(s) 284, 647
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 2 cut(s) 236, 623
Bse3DI GCAATG 1 cut(s) 261
BseBI CCWGG 1 cut(s) 867
BseDI CCNNGG 1 cut(s) 79
BseGI GGATG 1 cut(s) 385
BseLI CCNNNNNNNGG 2 cut(s) 284, 647
BseMI GCAATG 1 cut(s) 261
BseMII CTCAG 2 cut(s) 389, 800
BseNI ACTGG 2 cut(s) 236, 623
BseSI GKGCMC 1 cut(s) 696
BsgI GTGCAG 2 cut(s) 564, 705
Bsh1285I CGRYCG 1 cut(s) 343
BshFI GGCC 3 cut(s) 29, 591, 791
BsiEI CGRYCG 1 cut(s) 343
BsiSI CCGG 2 cut(s) 26, 768
BslI CCNNNNNNNGG 2 cut(s) 284, 647
BsmAI GTCTC 3 cut(s) 325, 504, 752
BsmI GAATGC 1 cut(s) 209
BsnI GGCC 3 cut(s) 29, 591, 791
Bso31I GGTCTC 1 cut(s) 504
Bsp1286I GDGCHC 1 cut(s) 696
Bsp143I GATC 6 cut(s) 75, 96, 298, 340, 429, 825
BspACI CCGC 1 cut(s) 84
BspANI GGCC 3 cut(s) 29, 591, 791
BspCNI CTCAG 2 cut(s) 390, 799
BspLI GGNNCC 2 cut(s) 77, 871
BspPI GGATC 4 cut(s) 70, 83, 306, 437
BspTNI GGTCTC 1 cut(s) 504
BsrDI GCAATG 1 cut(s) 261
BsrFI RCCGGY 1 cut(s) 25
BsrI ACTGG 2 cut(s) 236, 623
BssAI RCCGGY 1 cut(s) 25
BssECI CCNNGG 1 cut(s) 79
BssMI GATC 6 cut(s) 75, 96, 298, 340, 429, 825
BssNI GRCGYC 1 cut(s) 23
BssT1I CCWWGG 1 cut(s) 79
Bst2UI CCWGG 1 cut(s) 867
Bst4CI ACNGT 5 cut(s) 17, 128, 205, 421, 482
Bst6I CTCTTC 1 cut(s) 445
BstACI GRCGYC 1 cut(s) 23
BstAPI GCANNNNNTGC 3 cut(s) 413, 658, 728
BstBAI YACGTR 1 cut(s) 518
BstC8I GCNNGC 2 cut(s) 27, 773
BstDEI CTNAG 5 cut(s) 65, 169, 398, 578, 786
BstF5I GGATG 1 cut(s) 385
BstKTI GATC 6 cut(s) 78, 99, 301, 343, 432, 828
BstMAI GTCTC 3 cut(s) 325, 504, 752
BstMBI GATC 6 cut(s) 75, 96, 298, 340, 429, 825
BstMCI CGRYCG 1 cut(s) 343
BstMWI GCNNNNNNNGC 3 cut(s) 413, 658, 728
BstNI CCWGG 1 cut(s) 867
BstPAI GACNNNNGTC 1 cut(s) 874
BstSCI CCNGG 1 cut(s) 865
BstSFI CTRYAG 1 cut(s) 204
BstSLI GKGCMC 1 cut(s) 696
BstX2I RGATCY 1 cut(s) 75
BstYI RGATCY 1 cut(s) 75
BsuRI GGCC 3 cut(s) 29, 591, 791
BtsCI GGATG 1 cut(s) 385
BtsIMutI CAGTG 3 cut(s) 201, 229, 487
Cac8I GCNNGC 2 cut(s) 27, 773
Cfr10I RCCGGY 1 cut(s) 25
Cfr13I GGNCC 3 cut(s) 28, 590, 869
CseI GACGC 1 cut(s) 31
CviAII CATG 4 cut(s) 163, 189, 356, 823
DdeI CTNAG 5 cut(s) 65, 169, 398, 578, 786
DpnI GATC 6 cut(s) 77, 98, 300, 342, 431, 827
DpnII GATC 6 cut(s) 75, 96, 298, 340, 429, 825
Eam1104I CTCTTC 1 cut(s) 445
EarI CTCTTC 1 cut(s) 445
Eco130I CCWWGG 1 cut(s) 79
Eco147I AGGCCT 1 cut(s) 791
Eco31I GGTCTC 1 cut(s) 504
Eco47I GGWCC 1 cut(s) 869
EcoO109I RGGNCCY 1 cut(s) 869
EcoRI GAATTC 1 cut(s) 131
EcoRII CCWGG 1 cut(s) 865
EcoT14I CCWWGG 1 cut(s) 79
EcoT22I ATGCAT 1 cut(s) 190
ErhI CCWWGG 1 cut(s) 79
FaeI CATG 4 cut(s) 166, 192, 359, 826
FatI CATG 4 cut(s) 162, 188, 355, 822
FauI CCCGC 1 cut(s) 77
FauNDI CATATG 1 cut(s) 184
FbaI TGATCA 2 cut(s) 96, 825
FokI GGATG 1 cut(s) 372
FspBI CTAG 2 cut(s) 80, 807
HaeIII GGCC 3 cut(s) 29, 591, 791
HapII CCGG 2 cut(s) 26, 768
HgaI GACGC 1 cut(s) 31
Hin1I GRCGYC 1 cut(s) 23
Hin1II CATG 4 cut(s) 166, 192, 359, 826
HinfI GANTC 2 cut(s) 471, 485
HpaII CCGG 2 cut(s) 26, 768
HphI GGTGA 3 cut(s) 140, 539, 623
Hpy166II GTNNAC 2 cut(s) 39, 631
Hpy188I TCNGA 3 cut(s) 46, 219, 390
Hpy188III TCNNGA 3 cut(s) 117, 147, 278
Hpy8I GTNNAC 2 cut(s) 39, 631
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 1 cut(s) 723
HpyCH4III ACNGT 5 cut(s) 17, 128, 205, 421, 482
HpyCH4IV ACGT 1 cut(s) 517
HpyCH4V TGCA 7 cut(s) 4, 188, 254, 407, 545, 551, 722
HpyF10VI GCNNNNNNNGC 3 cut(s) 413, 658, 728
HpyF3I CTNAG 5 cut(s) 65, 169, 398, 578, 786
HpySE526I ACGT 1 cut(s) 517
Hsp92I GRCGYC 1 cut(s) 23
Hsp92II CATG 4 cut(s) 166, 192, 359, 826
KroI GCCGGC 1 cut(s) 25
KroNI GCCGGC 1 cut(s) 27
Ksp22I TGATCA 2 cut(s) 96, 825
Kzo9I GATC 6 cut(s) 75, 96, 298, 340, 429, 825
LmnI GCTCC 1 cut(s) 359
LweI GCATC 3 cut(s) 241, 458, 538
MaeI CTAG 2 cut(s) 80, 807
MaeII ACGT 1 cut(s) 517
MaeIII GTNAC 1 cut(s) 223
MalI GATC 6 cut(s) 77, 98, 300, 342, 431, 827
MboI GATC 6 cut(s) 75, 96, 298, 340, 429, 825
MboII GAAGA 3 cut(s) 20, 59, 432
MfeI CAATTG 1 cut(s) 552
MflI RGATCY 1 cut(s) 75
MhlI GDGCHC 1 cut(s) 696
MmeI TCCRAC 1 cut(s) 836
MnlI CCTC 6 cut(s) 27, 62, 420, 489, 523, 681
Mph1103I ATGCAT 1 cut(s) 190
MroNI GCCGGC 1 cut(s) 25
MseI TTAA 1 cut(s) 153
MslI CAYNNNNRTG 1 cut(s) 187
MspI CCGG 2 cut(s) 26, 768
MspR9I CCNGG 1 cut(s) 867
MunI CAATTG 1 cut(s) 552
Mva1269I GAATGC 1 cut(s) 209
MvaI CCWGG 1 cut(s) 867
MwoI GCNNNNNNNGC 3 cut(s) 413, 658, 728
NaeI GCCGGC 1 cut(s) 27
NdeI CATATG 1 cut(s) 184
NdeII GATC 6 cut(s) 75, 96, 298, 340, 429, 825
NgoMIV GCCGGC 1 cut(s) 25
NlaIII CATG 4 cut(s) 166, 192, 359, 826
NlaIV GGNNCC 2 cut(s) 77, 871
NmeAIII GCCGAG 1 cut(s) 302
NsiI ATGCAT 1 cut(s) 190
PceI AGGCCT 1 cut(s) 791
PctI GAATGC 1 cut(s) 209
PdiI GCCGGC 1 cut(s) 27
PfeI GAWTC 2 cut(s) 471, 485
PflMI CCANNNNNTGG 1 cut(s) 284
PfoI TCCNGGA 1 cut(s) 865
Ple19I CGATCG 1 cut(s) 343
Ppu21I YACGTR 1 cut(s) 518
PpuMI RGGWCCY 1 cut(s) 869
PshAI GACNNNNGTC 1 cut(s) 874
Psp5II RGGWCCY 1 cut(s) 869
Psp6I CCWGG 1 cut(s) 865
PspGI CCWGG 1 cut(s) 865
PspN4I GGNNCC 2 cut(s) 77, 871
PspPI GGNCC 3 cut(s) 28, 590, 869
PspPPI RGGWCCY 1 cut(s) 869
PsuI RGATCY 1 cut(s) 75
PvuI CGATCG 1 cut(s) 343
RseI CAYNNNNRTG 1 cut(s) 187
SaqAI TTAA 1 cut(s) 153
Sau3AI GATC 6 cut(s) 75, 96, 298, 340, 429, 825
Sau96I GGNCC 3 cut(s) 28, 590, 869
ScrFI CCNGG 1 cut(s) 867
SduI GDGCHC 1 cut(s) 696
SfaNI GCATC 3 cut(s) 241, 458, 538
SfcI CTRYAG 1 cut(s) 204
SinI GGWCC 1 cut(s) 869
SmiMI CAYNNNNRTG 1 cut(s) 187
SmlI CTYRAG 1 cut(s) 115
SmoI CTYRAG 1 cut(s) 115
SseBI AGGCCT 1 cut(s) 791
SsiI CCGC 1 cut(s) 84
SspI AATATT 1 cut(s) 103
SspMI CTAG 2 cut(s) 80, 807
StuI AGGCCT 1 cut(s) 791
StyD4I CCNGG 1 cut(s) 865
StyI CCWWGG 1 cut(s) 79
TaaI ACNGT 5 cut(s) 17, 128, 205, 421, 482
TaiI ACGT 1 cut(s) 520
TfiI GAWTC 2 cut(s) 471, 485
Tru1I TTAA 1 cut(s) 153
Tru9I TTAA 1 cut(s) 153
TscAI CASTG 3 cut(s) 208, 236, 487
TspDTI ATGAA 3 cut(s) 171, 179, 597
TspGWI ACGGA 2 cut(s) 88, 203
TspRI CASTG 3 cut(s) 208, 236, 487
Van91I CCANNNNNTGG 1 cut(s) 284
VpaK11BI GGWCC 1 cut(s) 869
XapI RAATTY 3 cut(s) 131, 832, 861
XmaJI CCTAGG 1 cut(s) 79
XspI CTAG 2 cut(s) 80, 807
Zsp2I ATGCAT 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.