Prupe.1G093200_v2.0.a1

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000533
Physical Location & Seq
Reverse (-)
7121592 .. 7125109
3518 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G093200.1

Sequence Viewer

Length: 1638 bp
ATGGAAAAGTCAAGAATTGGTGTTGTATTGTTTCTTTTGTACCTGTCTGTCTTCTATCCTCAATCTGAGGTTCAATCATTCGCCACTCCATCTGATCACGATTTAAGCTACATGAAATTTGTCTATAATTCCACTGATCTGCCATTAGAAGAAGTGTACGACTACATTGTAGTTGGAGGGGGTACAGCAGGCTGCCCATTAGCAGCAACTTTATCTGCAAAGTACTCGGTTCTCCTGCTAGAAAAGGGCAGTGTTCCAAGTGCATATCCAAGTGTTTTGCGCCAAGATGGGATTATTACAACTCTCGCGCTAGAAGACGATGGAAAGACGCCAGCTGAAAGGTTCACATCCGAAGATGGTGTGGCCAATGTAAGAGGCAGGGTTCTTGGTGGGTCAAGCATGATCAACACTGGTATATACTCTAGAGCCGAAAAAGAATTTTATGGAAACTCAGGAATTGAATGGGACATGGATTTGGTTAACAAGGCCTATCAATGGGTTGAAAATACTGTCATATCCCCCCAGAATGTGTCGCCTTGGCAATCTGTTGTAAAGGAAGGTTTGTTAGAGGCTGGTGTTTGTCCAGACAACGGATATAATTTAAATCACATTCCAGGAAGTAAGGTCACGGGTACTCTTTTTGACAATCAGGGAAGGAGACATGGAGCTGTGGAATTGCTTAATCTAGGAAACCCAAAGAACTTGCGAGTTGCAGTTCATGCCACAGTAGAGAGAATCATATTCTCTTCCAACGCACTAAGTTTGTCTGCTAGAGGAATCGTGTATAGTGATTCTAAAGGGAGGTCACATAAGGCCTTCATACATGGCAAGGGAGAGGTTATATTGAGTGCAGGAGCAATTGGAAGCACTCAACTCCTGCTACTTAGTGGTGTTGGCCCAGAGCCCTACCTTTCATCTATCAAAATCCCAGTTGTTCACCCCGAGCCTTACATTGGACAATTTATGCGTGATAATCCTCGTAATTACATCACAATTTTGCCTCCATTTCAACTGGATGCCTCTACTCCACAGATTGCTGGTATCACAAGCGATTTCTACATAGAGACTTTCTCGGGGTTACCATTTTCTACTCCACCATTTAGTGTTTTTCCTGATCCATCTTTCTCCACAAAGATAAATTCAACTTTCGGACAAATTGCGTACAAAATTCCAGGACCCTTGTCCCATGGTTCGCTTAGGCTGCAGTCGTCCTATGACGTCAAAGTTGGTCCAAATGTTCGCTTCAACTACTTTGCAAATCCGCTGGATCTTGCTCGTTGTGTTAGTGCCACAAGGAAGATCGGTGATTTATTAAGCACAAATTCGTTGAAACCATTTAAGGCTCAAGATTTGCCTGGTATAGATGGTTTCAACTTTTTCGGACCACCATTACCAATGAACCTTGCAGATACCGCATCCTTGGAAACATTTTGTCGAGAGACAGTAGCCACATTTTGGCACTACCATGGGGGATGCCTTGTGGGAAAGGTGGTTGATGGGGATTTACGAGTCAAGGGGATCAATGCCTTACGTGTTGTTGATGGATCCACATTCAAGTTCTCACCAGGGACCAATCCTCAGGCCACCCTTATGATGTTAGGAAGGTATATTGGCGTTAAGATGCTGAAAGAAAGATAA

Protein Analysis

546

Amino Acids

59.31

Weight (kDa)

6.8

Isoelectric Point (pI)

35.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1221
AccB7I CCANNNNNTGG 1 cut(s) 1455
AccII CGCG 1 cut(s) 308
AciI CCGC 2 cut(s) 1262, 1413
AclWI GGATC 5 cut(s) 1109, 1275, 1526, 1539, 1552
AcoI YGGCCR 1 cut(s) 363
AcsI RAATTY 5 cut(s) 116, 437, 1138, 1167, 1321
AcyI GRCGYC 2 cut(s) 329, 1218
AfaI GTAC 6 cut(s) 41, 158, 184, 224, 634, 1163
AfiI CCNNNNNNNGG 4 cut(s) 495, 590, 953, 1455
AflIII ACRYGT 1 cut(s) 1531
AgsI TTSAA 9 cut(s) 74, 461, 503, 1010, 1143, 1246, 1330, 1372, 1555
AjnI CCWGG 4 cut(s) 613, 1171, 1354, 1564
AluBI AGCT 3 cut(s) 108, 335, 668
AluI AGCT 3 cut(s) 108, 335, 668
Alw26I GTCTC 3 cut(s) 652, 1058, 1433
AlwI GGATC 5 cut(s) 1109, 1275, 1526, 1539, 1552
Ama87I CYCGRG 2 cut(s) 941, 1072
AoxI GGCC 5 cut(s) 363, 486, 813, 895, 1581
ApeKI GCWGC 3 cut(s) 192, 203, 1201
ApoI RAATTY 5 cut(s) 116, 437, 1138, 1167, 1321
AspLEI GCGC 2 cut(s) 282, 310
AspS9I GGNCC 5 cut(s) 896, 1175, 1229, 1382, 1569
AsuHPI GGTGA 3 cut(s) 929, 1316, 1554
AvaI CYCGRG 2 cut(s) 941, 1072
AvaII GGWCC 4 cut(s) 1175, 1229, 1382, 1569
AxyI CCTNAGG 1 cut(s) 1578
BalI TGGCCA 1 cut(s) 365
BamHI GGATCC 1 cut(s) 1544
BanII GRGCYC 1 cut(s) 906
BbsI GAAGAC 2 cut(s) 43, 321
BbvI GCAGC 3 cut(s) 179, 215, 1188
BccI CCATC 8 cut(s) 97, 281, 314, 350, 1126, 1358, 1490, 1535
BcgI CGANNNNNNTGC 2 cut(s) 207, 241
BciT130I CCWGG 4 cut(s) 615, 1173, 1356, 1566
BclI TGATCA 2 cut(s) 94, 402
BcoDI GTCTC 3 cut(s) 652, 1058, 1433
BfaI CTAG 5 cut(s) 239, 311, 423, 686, 771
BfmI CTRYAG 1 cut(s) 1202
BisI GCNGC 3 cut(s) 193, 204, 1202
BlsI GCNGC 3 cut(s) 194, 205, 1203
BmcAI AGTACT 1 cut(s) 224
Bme1390I CCNGG 4 cut(s) 615, 1173, 1356, 1566
Bme18I GGWCC 4 cut(s) 1175, 1229, 1382, 1569
BmeT110I CYCGRG 2 cut(s) 941, 1072
BmgT120I GGNCC 5 cut(s) 896, 1175, 1229, 1382, 1569
BmiI GGNNCC 3 cut(s) 1177, 1546, 1570
BmrFI CCNGG 4 cut(s) 615, 1173, 1356, 1566
BmrI ACTGGG 1 cut(s) 923
BmsI GCATC 4 cut(s) 1006, 1424, 1463, 1611
BmuI ACTGGG 1 cut(s) 923
BoxI GACNNNNGTC 1 cut(s) 1180
BpiI GAAGAC 2 cut(s) 43, 321
BplI GAGNNNNNCTC 2 cut(s) 1055, 1087
Bpu10I CCTNAGC 1 cut(s) 1196
BpuEI CTTGAG 1 cut(s) 1329
BsaAI YACGTR 1 cut(s) 1532
BsaHI GRCGYC 2 cut(s) 329, 1218
BsaJI CCNNGG 5 cut(s) 536, 1186, 1419, 1465, 1565
Bsc4I CCNNNNNNNGG 4 cut(s) 495, 590, 953, 1455
Bse1I ACTGG 3 cut(s) 415, 929, 1017
Bse21I CCTNAGG 1 cut(s) 1578
BseBI CCWGG 4 cut(s) 615, 1173, 1356, 1566
BseDI CCNNGG 5 cut(s) 536, 1186, 1419, 1465, 1565
BseGI GGATG 4 cut(s) 347, 1021, 1415, 1478
BseLI CCNNNNNNNGG 4 cut(s) 495, 590, 953, 1455
BseMII CTCAG 3 cut(s) 57, 465, 1592
BseNI ACTGG 3 cut(s) 415, 929, 1017
BseXI GCAGC 3 cut(s) 179, 215, 1188
BsgI GTGCAG 1 cut(s) 870
Bsh1236I CGCG 1 cut(s) 308
BshFI GGCC 5 cut(s) 365, 488, 815, 897, 1583
BsiHKCI CYCGRG 2 cut(s) 941, 1072
BslFI GGGAC 3 cut(s) 479, 1168, 1582
BslI CCNNNNNNNGG 4 cut(s) 495, 590, 953, 1455
BsmAI GTCTC 3 cut(s) 652, 1058, 1433
BsmFI GGGAC 3 cut(s) 479, 1168, 1582
BsnI GGCC 5 cut(s) 365, 488, 815, 897, 1583
BsoBI CYCGRG 2 cut(s) 941, 1072
Bsp1286I GDGCHC 1 cut(s) 906
Bsp143I GATC 8 cut(s) 94, 136, 402, 1114, 1267, 1299, 1518, 1544
Bsp19I CCATGG 2 cut(s) 1186, 1465
BspACI CCGC 2 cut(s) 1262, 1413
BspANI GGCC 5 cut(s) 365, 488, 815, 897, 1583
BspCNI CTCAG 3 cut(s) 58, 464, 1591
BspFNI CGCG 1 cut(s) 308
BspLI GGNNCC 3 cut(s) 1177, 1546, 1570
BspMAI CTGCAG 1 cut(s) 1206
BspPI GGATC 5 cut(s) 1109, 1275, 1526, 1539, 1552
BsrI ACTGG 3 cut(s) 415, 929, 1017
BssECI CCNNGG 5 cut(s) 536, 1186, 1419, 1465, 1565
BssMI GATC 8 cut(s) 94, 136, 402, 1114, 1267, 1299, 1518, 1544
BssNI GRCGYC 2 cut(s) 329, 1218
BssT1I CCWWGG 4 cut(s) 536, 1186, 1419, 1465
Bst2UI CCWGG 4 cut(s) 615, 1173, 1356, 1566
Bst4CI ACNGT 3 cut(s) 511, 727, 1444
Bst6I CTCTTC 1 cut(s) 751
BstACI GRCGYC 2 cut(s) 329, 1218
BstAPI GCANNNNNTGC 1 cut(s) 719
BstBAI YACGTR 1 cut(s) 1532
BstC8I GCNNGC 2 cut(s) 190, 333
BstDEI CTNAG 6 cut(s) 66, 451, 758, 884, 1196, 1578
BstDSI CCRYGG 2 cut(s) 1186, 1465
BstEII GGTNACC 1 cut(s) 1077
BstF5I GGATG 4 cut(s) 347, 1021, 1415, 1478
BstFNI CGCG 1 cut(s) 308
BstHHI GCGC 2 cut(s) 282, 310
BstKTI GATC 8 cut(s) 97, 139, 405, 1117, 1270, 1302, 1521, 1547
BstMAI GTCTC 3 cut(s) 652, 1058, 1433
BstMBI GATC 8 cut(s) 94, 136, 402, 1114, 1267, 1299, 1518, 1544
BstMWI GCNNNNNNNGC 3 cut(s) 719, 1201, 1412
BstNI CCWGG 4 cut(s) 615, 1173, 1356, 1566
BstPAI GACNNNNGTC 1 cut(s) 1180
BstPI GGTNACC 1 cut(s) 1077
BstSCI CCNGG 4 cut(s) 613, 1171, 1354, 1564
BstSFI CTRYAG 1 cut(s) 1202
BstUI CGCG 1 cut(s) 308
BstV1I GCAGC 3 cut(s) 179, 215, 1188
BstV2I GAAGAC 2 cut(s) 43, 321
BstX2I RGATCY 2 cut(s) 1267, 1544
BstYI RGATCY 2 cut(s) 1267, 1544
Bsu36I CCTNAGG 1 cut(s) 1578
BsuRI GGCC 5 cut(s) 365, 488, 815, 897, 1583
BtgI CCRYGG 2 cut(s) 1186, 1465
BtsCI GGATG 4 cut(s) 347, 1021, 1415, 1478
BtsI GCAGTG 1 cut(s) 256
BtsIMutI CAGTG 3 cut(s) 132, 256, 408
Cac8I GCNNGC 2 cut(s) 190, 333
CfoI GCGC 2 cut(s) 282, 310
Cfr13I GGNCC 5 cut(s) 896, 1175, 1229, 1382, 1569
CseI GACGC 1 cut(s) 337
Csp6I GTAC 6 cut(s) 40, 157, 183, 223, 633, 1162
CviAII CATG 8 cut(s) 112, 400, 469, 662, 719, 824, 1187, 1466
CviQI GTAC 6 cut(s) 40, 157, 183, 223, 633, 1162
DdeI CTNAG 6 cut(s) 66, 451, 758, 884, 1196, 1578
DpnI GATC 8 cut(s) 96, 138, 404, 1116, 1269, 1301, 1520, 1546
DpnII GATC 8 cut(s) 94, 136, 402, 1114, 1267, 1299, 1518, 1544
DraI TTTAAA 1 cut(s) 603
EaeI YGGCCR 1 cut(s) 363
Eam1104I CTCTTC 1 cut(s) 751
EarI CTCTTC 1 cut(s) 751
Eco130I CCWWGG 4 cut(s) 536, 1186, 1419, 1465
Eco147I AGGCCT 2 cut(s) 488, 815
Eco24I GRGCYC 1 cut(s) 906
Eco47I GGWCC 4 cut(s) 1175, 1229, 1382, 1569
Eco81I CCTNAGG 1 cut(s) 1578
Eco88I CYCGRG 2 cut(s) 941, 1072
Eco91I GGTNACC 1 cut(s) 1077
EcoO109I RGGNCCY 1 cut(s) 1175
EcoO65I GGTNACC 1 cut(s) 1077
EcoRII CCWGG 4 cut(s) 613, 1171, 1354, 1564
EcoT14I CCWWGG 4 cut(s) 536, 1186, 1419, 1465
EcoT38I GRGCYC 1 cut(s) 906
ErhI CCWWGG 4 cut(s) 536, 1186, 1419, 1465
FaeI CATG 8 cut(s) 115, 403, 472, 665, 722, 827, 1190, 1469
FaqI GGGAC 3 cut(s) 479, 1168, 1582
FatI CATG 8 cut(s) 111, 399, 468, 661, 718, 823, 1186, 1465
FbaI TGATCA 2 cut(s) 94, 402
Fnu4HI GCNGC 3 cut(s) 193, 204, 1202
FokI GGATG 4 cut(s) 334, 1028, 1402, 1485
FriOI GRGCYC 1 cut(s) 906
Fsp4HI GCNGC 3 cut(s) 193, 204, 1202
FspBI CTAG 5 cut(s) 239, 311, 423, 686, 771
GlaI GCGC 2 cut(s) 281, 309
GluI GCNGC 3 cut(s) 193, 204, 1202
HaeIII GGCC 5 cut(s) 365, 488, 815, 897, 1583
HgaI GACGC 1 cut(s) 337
HhaI GCGC 2 cut(s) 282, 310
Hin1I GRCGYC 2 cut(s) 329, 1218
Hin1II CATG 8 cut(s) 115, 403, 472, 665, 722, 827, 1190, 1469
Hin6I GCGC 2 cut(s) 280, 308
HinP1I GCGC 2 cut(s) 280, 308
HincII GTYRAC 1 cut(s) 481
HindII GTYRAC 1 cut(s) 481
HinfI GANTC 4 cut(s) 735, 777, 791, 1509
HpaI GTTAAC 1 cut(s) 481
HphI GGTGA 3 cut(s) 929, 1316, 1554
Hpy166II GTNNAC 4 cut(s) 157, 345, 481, 937
Hpy188I TCNGA 5 cut(s) 67, 94, 352, 1151, 1382
Hpy188III TCNNGA 8 cut(s) 12, 98, 423, 453, 584, 1112, 1346, 1436
Hpy8I GTNNAC 4 cut(s) 157, 345, 481, 937
HpyAV CCTTC 4 cut(s) 551, 648, 826, 1596
HpyCH4III ACNGT 3 cut(s) 511, 727, 1444
HpyCH4IV ACGT 2 cut(s) 1218, 1531
HpyCH4V TGCA 7 cut(s) 218, 263, 713, 851, 1204, 1256, 1406
HpyF10VI GCNNNNNNNGC 3 cut(s) 719, 1201, 1412
HpyF3I CTNAG 6 cut(s) 66, 451, 758, 884, 1196, 1578
HpySE526I ACGT 2 cut(s) 1218, 1531
Hsp92I GRCGYC 2 cut(s) 329, 1218
Hsp92II CATG 8 cut(s) 115, 403, 472, 665, 722, 827, 1190, 1469
HspAI GCGC 2 cut(s) 280, 308
Ksp22I TGATCA 2 cut(s) 94, 402
KspAI GTTAAC 1 cut(s) 481
Kzo9I GATC 8 cut(s) 94, 136, 402, 1114, 1267, 1299, 1518, 1544
LmnI GCTCC 2 cut(s) 665, 854
Lsp1109I GCAGC 3 cut(s) 179, 215, 1188
LweI GCATC 4 cut(s) 1006, 1424, 1463, 1611
MaeI CTAG 5 cut(s) 239, 311, 423, 686, 771
MaeII ACGT 2 cut(s) 1218, 1531
MaeIII GTNAC 3 cut(s) 625, 804, 1077
MalI GATC 8 cut(s) 96, 138, 404, 1116, 1269, 1301, 1520, 1546
MboI GATC 8 cut(s) 94, 136, 402, 1114, 1267, 1299, 1518, 1544
MboII GAAGA 6 cut(s) 43, 161, 326, 365, 738, 1309
MfeI CAATTG 1 cut(s) 858
MflI RGATCY 2 cut(s) 1267, 1544
MhlI GDGCHC 1 cut(s) 906
MlsI TGGCCA 1 cut(s) 365
MluNI TGGCCA 1 cut(s) 365
MlyI GAGTC 1 cut(s) 1518
MmeI TCCRAC 2 cut(s) 154, 774
Mox20I TGGCCA 1 cut(s) 365
MscI TGGCCA 1 cut(s) 365
MseI TTAA 7 cut(s) 104, 480, 602, 681, 1313, 1338, 1617
MslI CAYNNNNRTG 2 cut(s) 1464, 1589
Msp20I TGGCCA 1 cut(s) 365
MspA1I CMGCKG 2 cut(s) 335, 1264
MspR9I CCNGG 4 cut(s) 615, 1173, 1356, 1566
MunI CAATTG 1 cut(s) 858
MvaI CCWGG 4 cut(s) 615, 1173, 1356, 1566
MvnI CGCG 1 cut(s) 308
MwoI GCNNNNNNNGC 3 cut(s) 719, 1201, 1412
NcoI CCATGG 2 cut(s) 1186, 1465
NdeII GATC 8 cut(s) 94, 136, 402, 1114, 1267, 1299, 1518, 1544
NlaIII CATG 8 cut(s) 115, 403, 472, 665, 722, 827, 1190, 1469
NlaIV GGNNCC 3 cut(s) 1177, 1546, 1570
NmuCI GTSAC 2 cut(s) 625, 804
PceI AGGCCT 2 cut(s) 488, 815
PfeI GAWTC 3 cut(s) 735, 777, 791
PflMI CCANNNNNTGG 1 cut(s) 1455
PfoI TCCNGGA 2 cut(s) 613, 1171
PkrI GCNGC 3 cut(s) 194, 205, 1203
PleI GAGTC 1 cut(s) 1517
PpsI GAGTC 1 cut(s) 1517
Ppu21I YACGTR 1 cut(s) 1532
PpuMI RGGWCCY 1 cut(s) 1175
PshAI GACNNNNGTC 1 cut(s) 1180
Psp5II RGGWCCY 1 cut(s) 1175
Psp6I CCWGG 4 cut(s) 613, 1171, 1354, 1564
PspEI GGTNACC 1 cut(s) 1077
PspGI CCWGG 4 cut(s) 613, 1171, 1354, 1564
PspN4I GGNNCC 3 cut(s) 1177, 1546, 1570
PspPI GGNCC 5 cut(s) 896, 1175, 1229, 1382, 1569
PspPPI RGGWCCY 1 cut(s) 1175
PstI CTGCAG 1 cut(s) 1206
PsuI RGATCY 2 cut(s) 1267, 1544
PvuII CAGCTG 1 cut(s) 335
RsaI GTAC 6 cut(s) 41, 158, 184, 224, 634, 1163
RsaNI GTAC 6 cut(s) 40, 157, 183, 223, 633, 1162
RseI CAYNNNNRTG 2 cut(s) 1464, 1589
SaqAI TTAA 7 cut(s) 104, 480, 602, 681, 1313, 1338, 1617
SatI GCNGC 3 cut(s) 193, 204, 1202
Sau3AI GATC 8 cut(s) 94, 136, 402, 1114, 1267, 1299, 1518, 1544
Sau96I GGNCC 5 cut(s) 896, 1175, 1229, 1382, 1569
ScaI AGTACT 1 cut(s) 224
SchI GAGTC 1 cut(s) 1518
ScrFI CCNGG 4 cut(s) 615, 1173, 1356, 1566
SduI GDGCHC 1 cut(s) 906
SfaNI GCATC 4 cut(s) 1006, 1424, 1463, 1611
SfcI CTRYAG 1 cut(s) 1202
SinI GGWCC 4 cut(s) 1175, 1229, 1382, 1569
SmiI ATTTAAAT 1 cut(s) 603
SmiMI CAYNNNNRTG 2 cut(s) 1464, 1589
SmlI CTYRAG 1 cut(s) 1344
SmoI CTYRAG 1 cut(s) 1344
SseBI AGGCCT 2 cut(s) 488, 815
SsiI CCGC 2 cut(s) 1262, 1413
SspMI CTAG 5 cut(s) 239, 311, 423, 686, 771
StuI AGGCCT 2 cut(s) 488, 815
StyD4I CCNGG 4 cut(s) 613, 1171, 1354, 1564
StyI CCWWGG 4 cut(s) 536, 1186, 1419, 1465
SwaI ATTTAAAT 1 cut(s) 603
TaaI ACNGT 3 cut(s) 511, 727, 1444
TaiI ACGT 2 cut(s) 1221, 1534
TaqI TCGA 1 cut(s) 1435
TatI WGTACW 1 cut(s) 222
TfiI GAWTC 3 cut(s) 735, 777, 791
Tru1I TTAA 7 cut(s) 104, 480, 602, 681, 1313, 1338, 1617
Tru9I TTAA 7 cut(s) 104, 480, 602, 681, 1313, 1338, 1617
TscAI CASTG 3 cut(s) 139, 256, 415
TseFI GTSAC 2 cut(s) 625, 804
TseI GCWGC 3 cut(s) 192, 203, 1201
Tsp45I GTSAC 2 cut(s) 625, 804
TspDTI ATGAA 5 cut(s) 128, 707, 808, 903, 1412
TspGWI ACGGA 1 cut(s) 606
TspRI CASTG 3 cut(s) 139, 256, 415
Van91I CCANNNNNTGG 1 cut(s) 1455
VpaK11BI GGWCC 4 cut(s) 1175, 1229, 1382, 1569
XapI RAATTY 5 cut(s) 116, 437, 1138, 1167, 1321
XbaI TCTAGA 1 cut(s) 422
XspI CTAG 5 cut(s) 239, 311, 423, 686, 771
ZraI GACGTC 1 cut(s) 1219
ZrmI AGTACT 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.