Rmu_co8470309.1_g000001

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8470309.1
OG0000533
Physical Location & Seq
Forward (+)
321 .. 1662
1342 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8470309.1_g000001.1.cds

Sequence Viewer

Length: 1032 bp
atgggaactaaatttggtggttctaattttgatagacatggaaggagacacggagctgttgaatttcttaacagaggaaacgtaaagaacttgcgagtaggaattcatgccatagtcgagaagatcatcttctcttccaaaggatcaagtatttcggctcgaggggtgatatatactgattccaatgggaggtctcacggggcatttgtacgtggtgagggagaggttattctgagtgcaggggcaattgggagtcctcagcttctacttcttagcggtgttggccctcagtcctatctatcatctttgaaaatcctagttgttcacccccaacctcacagtggaaactttatgtatgacaatcctcttaattttattaccattttatccccgtttccactacaggcctcaactgtaaagattgtcggcattaccgaccatttctacgtagagaccttctctagcatgcccatttctactacaccctatagtctttttccagatccggctaaacttataaggataaattcaagtttaggactcatttacatcaaattggtaggaccttcgtcttatggtactctgaagcttcaatcatcatctgatgtgagagttggtccaaatgtccgcctcaactactttgcacatcccttggaccttgctcgctgtgtttctggcatgaagaagattggggagttattaaataccaagtcactgaaagcatttaagtttctagcttcgtcgagcacaaaagcttacaagttttatggtccaactttacctatgaaccaaacagatgatgcatcttttggagaattttgtcgtcatacagtatcaacactttggcatttccatggaggatgcctggtcggaaaggtggtcgatagcagcttgcaggtcatgggaatcgatgctttgcgtgttgttgacgcatccacattcaatttcacaccggggacaaatccacaggccacacttatgatgttgggcaggtacattggcattaagatgctgcaacaaagatcttcttaa

Protein Analysis

343

Amino Acids

37.25

Weight (kDa)

9.81

Isoelectric Point (pI)

32.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 518
Acc36I ACCTGC 2 cut(s) 886, 981
AciI CCGC 2 cut(s) 276, 628
AclWI GGATC 2 cut(s) 151, 497
AcsI RAATTY 5 cut(s) 11, 62, 102, 526, 815
AcuI CTGAAG 1 cut(s) 605
AfaI GTAC 3 cut(s) 210, 580, 995
AfiI CCNNNNNNNGG 2 cut(s) 189, 341
AgsI TTSAA 5 cut(s) 62, 310, 531, 593, 943
AjnI CCWGG 1 cut(s) 864
AluBI AGCT 6 cut(s) 56, 262, 589, 737, 755, 891
AluI AGCT 6 cut(s) 56, 262, 589, 737, 755, 891
Alw21I GWGCWC 1 cut(s) 749
Alw26I GTCTC 3 cut(s) 40, 198, 446
AlwI GGATC 2 cut(s) 151, 497
Ama87I CYCGRG 1 cut(s) 159
AoxI GGCC 3 cut(s) 283, 405, 969
ApeKI GCWGC 2 cut(s) 888, 1012
ApoI RAATTY 5 cut(s) 11, 62, 102, 526, 815
AspS9I GGNCC 5 cut(s) 284, 563, 617, 655, 770
AsuC2I CCSGG 1 cut(s) 954
AsuHPI GGTGA 3 cut(s) 178, 227, 317
AvaI CYCGRG 1 cut(s) 159
AvaII GGWCC 4 cut(s) 563, 617, 655, 770
Bbv12I GWGCWC 1 cut(s) 749
BbvCI CCTCAGC 1 cut(s) 258
BbvI GCAGC 2 cut(s) 900, 999
BciT130I CCWGG 1 cut(s) 866
BcnI CCSGG 1 cut(s) 954
BcoDI GTCTC 3 cut(s) 40, 198, 446
BfaI CTAG 3 cut(s) 317, 462, 734
BfmI CTRYAG 2 cut(s) 401, 487
BfuAI ACCTGC 2 cut(s) 886, 981
BglII AGATCT 1 cut(s) 1022
BisI GCNGC 2 cut(s) 889, 1013
BlsI GCNGC 2 cut(s) 890, 1014
Bme1390I CCNGG 2 cut(s) 866, 954
Bme18I GGWCC 4 cut(s) 563, 617, 655, 770
BmeT110I CYCGRG 1 cut(s) 159
BmgT120I GGNCC 5 cut(s) 284, 563, 617, 655, 770
BmrFI CCNGG 2 cut(s) 866, 954
BmsI GCATC 6 cut(s) 790, 812, 851, 901, 941, 999
BoxI GACNNNNGTC 1 cut(s) 568
BplI GAGNNNNNCTC 2 cut(s) 443, 475
Bpu10I CCTNAGC 1 cut(s) 258
BpuMI CCSGG 1 cut(s) 954
Bsa29I ATCGAT 1 cut(s) 909
BsaAI YACGTR 2 cut(s) 212, 448
BsaI GGTCTC 2 cut(s) 198, 446
BsaJI CCNNGG 3 cut(s) 651, 853, 953
Bsc4I CCNNNNNNNGG 2 cut(s) 189, 341
BseBI CCWGG 1 cut(s) 866
BseCI ATCGAT 1 cut(s) 909
BseDI CCNNGG 3 cut(s) 651, 853, 953
BseGI GGATG 3 cut(s) 646, 866, 932
BseLI CCNNNNNNNGG 2 cut(s) 189, 341
BseMII CTCAG 3 cut(s) 224, 272, 302
BseXI GCAGC 2 cut(s) 900, 999
BsgI GTGCAG 1 cut(s) 258
BshFI GGCC 3 cut(s) 285, 407, 971
BshVI ATCGAT 1 cut(s) 909
BsiHKAI GWGCWC 1 cut(s) 749
BsiHKCI CYCGRG 1 cut(s) 159
BsiSI CCGG 2 cut(s) 506, 953
BslFI GGGAC 1 cut(s) 970
BslI CCNNNNNNNGG 2 cut(s) 189, 341
BsmAI GTCTC 3 cut(s) 40, 198, 446
BsmFI GGGAC 1 cut(s) 970
BsnI GGCC 3 cut(s) 285, 407, 971
Bso31I GGTCTC 2 cut(s) 198, 446
BsoBI CYCGRG 1 cut(s) 159
Bsp1286I GDGCHC 1 cut(s) 749
Bsp143I GATC 4 cut(s) 123, 143, 502, 1022
Bsp19I CCATGG 1 cut(s) 853
BspACI CCGC 2 cut(s) 276, 628
BspANI GGCC 3 cut(s) 285, 407, 971
BspCNI CTCAG 3 cut(s) 225, 271, 301
BspDI ATCGAT 1 cut(s) 909
BspMI ACCTGC 2 cut(s) 886, 981
BspPI GGATC 2 cut(s) 151, 497
BspTNI GGTCTC 2 cut(s) 198, 446
BssECI CCNNGG 3 cut(s) 651, 853, 953
BssMI GATC 4 cut(s) 123, 143, 502, 1022
BssT1I CCWWGG 2 cut(s) 651, 853
Bst2UI CCWGG 1 cut(s) 866
Bst4CI ACNGT 3 cut(s) 341, 415, 832
Bst6I CTCTTC 1 cut(s) 139
BstBAI YACGTR 2 cut(s) 212, 448
BstC8I GCNNGC 3 cut(s) 467, 664, 893
BstDEI CTNAG 4 cut(s) 233, 258, 272, 288
BstDSI CCRYGG 1 cut(s) 853
BstF5I GGATG 3 cut(s) 646, 866, 932
BstKTI GATC 4 cut(s) 126, 146, 505, 1025
BstMAI GTCTC 3 cut(s) 40, 198, 446
BstMBI GATC 4 cut(s) 123, 143, 502, 1022
BstMWI GCNNNNNNNGC 1 cut(s) 282
BstNI CCWGG 1 cut(s) 866
BstNSI RCATGY 1 cut(s) 469
BstPAI GACNNNNGTC 1 cut(s) 568
BstSCI CCNGG 2 cut(s) 864, 952
BstSFI CTRYAG 2 cut(s) 401, 487
BstSNI TACGTA 1 cut(s) 448
BstV1I GCAGC 2 cut(s) 900, 999
BstX2I RGATCY 2 cut(s) 502, 1022
BstYI RGATCY 2 cut(s) 502, 1022
Bsu15I ATCGAT 1 cut(s) 909
BsuRI GGCC 3 cut(s) 285, 407, 971
BsuTUI ATCGAT 1 cut(s) 909
BtgI CCRYGG 1 cut(s) 853
BtsCI GGATG 3 cut(s) 646, 866, 932
BtsIMutI CAGTG 2 cut(s) 346, 713
BveI ACCTGC 2 cut(s) 886, 981
Cac8I GCNNGC 3 cut(s) 467, 664, 893
Cfr13I GGNCC 5 cut(s) 284, 563, 617, 655, 770
ClaI ATCGAT 1 cut(s) 909
CseI GACGC 1 cut(s) 938
Csp6I GTAC 3 cut(s) 209, 579, 994
CviAII CATG 6 cut(s) 38, 107, 466, 679, 854, 901
CviQI GTAC 3 cut(s) 209, 579, 994
DdeI CTNAG 4 cut(s) 233, 258, 272, 288
DpnI GATC 4 cut(s) 125, 145, 504, 1024
DpnII GATC 4 cut(s) 123, 143, 502, 1022
Eam1104I CTCTTC 1 cut(s) 139
EarI CTCTTC 1 cut(s) 139
EciI GGCGGA 1 cut(s) 617
Eco105I TACGTA 1 cut(s) 448
Eco130I CCWWGG 2 cut(s) 651, 853
Eco147I AGGCCT 1 cut(s) 407
Eco31I GGTCTC 2 cut(s) 198, 446
Eco47I GGWCC 4 cut(s) 563, 617, 655, 770
Eco57I CTGAAG 1 cut(s) 605
Eco88I CYCGRG 1 cut(s) 159
EcoO109I RGGNCCY 1 cut(s) 563
EcoRI GAATTC 1 cut(s) 102
EcoRII CCWGG 1 cut(s) 864
EcoT14I CCWWGG 2 cut(s) 651, 853
EcoT22I ATGCAT 1 cut(s) 805
ErhI CCWWGG 2 cut(s) 651, 853
FaeI CATG 6 cut(s) 41, 110, 469, 682, 857, 904
FalI AAGNNNNNCTT 3 cut(s) 113, 145, 1012
FaqI GGGAC 1 cut(s) 970
FatI CATG 6 cut(s) 37, 106, 465, 678, 853, 900
Fnu4HI GCNGC 2 cut(s) 889, 1013
FokI GGATG 3 cut(s) 633, 873, 919
Fsp4HI GCNGC 2 cut(s) 889, 1013
FspBI CTAG 3 cut(s) 317, 462, 734
GluI GCNGC 2 cut(s) 889, 1013
HaeIII GGCC 3 cut(s) 285, 407, 971
HapII CCGG 2 cut(s) 506, 953
HgaI GACGC 1 cut(s) 938
Hin1II CATG 6 cut(s) 41, 110, 469, 682, 857, 904
HincII GTYRAC 1 cut(s) 928
HindII GTYRAC 1 cut(s) 928
HindIII AAGCTT 2 cut(s) 587, 753
HinfI GANTC 4 cut(s) 179, 253, 540, 906
HpaII CCGG 2 cut(s) 506, 953
HphI GGTGA 3 cut(s) 178, 227, 317
Hpy166II GTNNAC 2 cut(s) 325, 928
Hpy188I TCNGA 4 cut(s) 234, 585, 604, 872
Hpy188III TCNNGA 2 cut(s) 118, 500
Hpy8I GTNNAC 2 cut(s) 325, 928
Hpy99I CGWCG 1 cut(s) 745
HpyAV CCTTC 3 cut(s) 36, 466, 576
HpyCH4III ACNGT 3 cut(s) 341, 415, 832
HpyCH4IV ACGT 3 cut(s) 81, 211, 447
HpyCH4V TGCA 5 cut(s) 239, 644, 803, 895, 1015
HpyF10VI GCNNNNNNNGC 1 cut(s) 282
HpyF3I CTNAG 4 cut(s) 233, 258, 272, 288
HpySE526I ACGT 3 cut(s) 81, 211, 447
Hsp92II CATG 6 cut(s) 41, 110, 469, 682, 857, 904
Kzo9I GATC 4 cut(s) 123, 143, 502, 1022
LmnI GCTCC 1 cut(s) 53
Lsp1109I GCAGC 2 cut(s) 900, 999
LweI GCATC 6 cut(s) 790, 812, 851, 901, 941, 999
MaeI CTAG 3 cut(s) 317, 462, 734
MaeII ACGT 3 cut(s) 81, 211, 447
MaeIII GTNAC 1 cut(s) 711
MalI GATC 4 cut(s) 125, 145, 504, 1024
MboI GATC 4 cut(s) 123, 143, 502, 1022
MboII GAAGA 6 cut(s) 121, 126, 133, 694, 697, 1017
MfeI CAATTG 1 cut(s) 246
MflI RGATCY 2 cut(s) 502, 1022
MhlI GDGCHC 1 cut(s) 749
MlyI GAGTC 2 cut(s) 262, 534
MmeI TCCRAC 2 cut(s) 797, 850
Mph1103I ATGCAT 1 cut(s) 805
MseI TTAA 6 cut(s) 69, 369, 701, 726, 1005, 1030
MslI CAYNNNNRTG 3 cut(s) 852, 977, 1007
MspI CCGG 2 cut(s) 506, 953
MspR9I CCNGG 2 cut(s) 866, 954
MunI CAATTG 1 cut(s) 246
MvaI CCWGG 1 cut(s) 866
MwoI GCNNNNNNNGC 1 cut(s) 282
NciI CCSGG 1 cut(s) 954
NcoI CCATGG 1 cut(s) 853
NdeII GATC 4 cut(s) 123, 143, 502, 1022
NlaIII CATG 6 cut(s) 41, 110, 469, 682, 857, 904
NmuCI GTSAC 1 cut(s) 711
NsiI ATGCAT 1 cut(s) 805
NspI RCATGY 1 cut(s) 469
PaeI GCATGC 1 cut(s) 469
PaeR7I CTCGAG 1 cut(s) 159
PceI AGGCCT 1 cut(s) 407
PcsI WCGNNNNNNNCGW 1 cut(s) 432
PfeI GAWTC 2 cut(s) 179, 906
PkrI GCNGC 2 cut(s) 890, 1014
PleI GAGTC 2 cut(s) 261, 534
PpsI GAGTC 2 cut(s) 261, 534
Ppu21I YACGTR 2 cut(s) 212, 448
PpuMI RGGWCCY 1 cut(s) 563
PshAI GACNNNNGTC 1 cut(s) 568
PsiI TTATAA 1 cut(s) 518
Psp5II RGGWCCY 1 cut(s) 563
Psp6I CCWGG 1 cut(s) 864
PspGI CCWGG 1 cut(s) 864
PspPI GGNCC 5 cut(s) 284, 563, 617, 655, 770
PspPPI RGGWCCY 1 cut(s) 563
PspXI VCTCGAGB 1 cut(s) 159
PsuI RGATCY 2 cut(s) 502, 1022
RsaI GTAC 3 cut(s) 210, 580, 995
RsaNI GTAC 3 cut(s) 209, 579, 994
RseI CAYNNNNRTG 3 cut(s) 852, 977, 1007
SaqAI TTAA 6 cut(s) 69, 369, 701, 726, 1005, 1030
SatI GCNGC 2 cut(s) 889, 1013
Sau3AI GATC 4 cut(s) 123, 143, 502, 1022
Sau96I GGNCC 5 cut(s) 284, 563, 617, 655, 770
SchI GAGTC 2 cut(s) 262, 534
ScrFI CCNGG 2 cut(s) 866, 954
SduI GDGCHC 1 cut(s) 749
SfaNI GCATC 6 cut(s) 790, 812, 851, 901, 941, 999
SfcI CTRYAG 2 cut(s) 401, 487
Sfr274I CTCGAG 1 cut(s) 159
SinI GGWCC 4 cut(s) 563, 617, 655, 770
SlaI CTCGAG 1 cut(s) 159
SmiMI CAYNNNNRTG 3 cut(s) 852, 977, 1007
SmlI CTYRAG 1 cut(s) 159
SmoI CTYRAG 1 cut(s) 159
SnaBI TACGTA 1 cut(s) 448
SphI GCATGC 1 cut(s) 469
SseBI AGGCCT 1 cut(s) 407
SsiI CCGC 2 cut(s) 276, 628
SspMI CTAG 3 cut(s) 317, 462, 734
StuI AGGCCT 1 cut(s) 407
StyD4I CCNGG 2 cut(s) 864, 952
StyI CCWWGG 2 cut(s) 651, 853
TaaI ACNGT 3 cut(s) 341, 415, 832
TaiI ACGT 3 cut(s) 84, 214, 450
TaqI TCGA 5 cut(s) 117, 160, 743, 882, 909
TfiI GAWTC 2 cut(s) 179, 906
Tru1I TTAA 6 cut(s) 69, 369, 701, 726, 1005, 1030
Tru9I TTAA 6 cut(s) 69, 369, 701, 726, 1005, 1030
TscAI CASTG 2 cut(s) 346, 720
TseFI GTSAC 1 cut(s) 711
TseI GCWGC 2 cut(s) 888, 1012
Tsp45I GTSAC 1 cut(s) 711
TspDTI ATGAA 3 cut(s) 95, 695, 800
TspGWI ACGGA 1 cut(s) 66
TspRI CASTG 2 cut(s) 346, 720
VpaK11BI GGWCC 4 cut(s) 563, 617, 655, 770
XapI RAATTY 5 cut(s) 11, 62, 102, 526, 815
XceI RCATGY 1 cut(s) 469
XcmI CCANNNNNNNNNTGG 1 cut(s) 338
XhoI CTCGAG 1 cut(s) 159
XspI CTAG 3 cut(s) 317, 462, 734
Zsp2I ATGCAT 1 cut(s) 805
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.