Rmu_sc0004115.1_g000016

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004115.1
OG0000533
Physical Location & Seq
Reverse (-)
84184 .. 90042
5859 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004115.1_g000016.1.cds

Sequence Viewer

Length: 1806 bp
atgccgtttcctctagggtatcagaagcaactaggcctatcatctatggcagaagccgtggtagccaaatacctaagttcagtctcagaagaaggggccatgaccaccatgttaggtaaacaagccatgaaagttgtatcaaaggctccccaatgtggtggaccatctgcaccgacaaaactagccttgtctatagcaaatctcaccgggaccttttttcattcattggccaatgcatctgcacctaatcatgattttagctacatgaagattgtgaaggatgccactgatctaccgttggaagataaatatgactatattgtagttgggggaggaacagcaggttgtccattagcagcaacattatcagcaagctactcggttcttgttcttgaaaggggtagtgctcctactgcacatccaagtgtcttgcgagaagaagggtttctgagtaatctcatgcaagaagatgatggtaagatcacaccagctcaaaagtttaagtctgaagacggtgttgctattgtcagaggaagggttcttggtggctcaagcatgatcaatgctggtttctactccaaagctgacagggaattctttgttaaatctggaattaaatgggacatggatttggttgataaggcatatcactgggttgaaagcactgtcgtctctcgtccaaatctgtcacgttcaaattggcaatctattgtgaaagaagcattgttggaggctggtgttggtccaaacaatggattcagtttggatcacattaagggaactaaagttggcggttctacttttgatacacatggaaggagatacggagctgttgaattgcttaacagaggaaacctaaagaacttgcgagtgggaattcatgccacagtcgagaaaatcatcttctcttccaaagaatcaggtgtttcggctcgaggggtggtatatattgattctaatgggaggtctcatcgggcatttgtacgtggtaagggagaggttattctgagtgcaggggcaattgggagtcctcagcttctacttcttagcggtgttggtcctcagtcctatctatcatctttgaaaatcccagttgttcacccccaacctcacactggaaactttatgtatgacaatcctcgtaatttcattaacattttgtccccgtttccactacagccctcaactgtaaagattgtcggcattaccgaccatttctacatagagaccctctctagcatgcccatttctactacaccctacagtgtttttccagatccggctaaacttacaaagataaattcaagtttaggactcattgccatcaaattagtaggacctttgtcttatggtactttgaagcttcaatcatcatctgatgtgagagttggtccaaatgtccgcttcaactactttgcacatcccttggaccttgctcgttgtgtttctggcatgaagaagattggggagttattaaatacgaagtcactgaaagcatttaaggttcaagcttcgtcgagcacaaaagcttacaatttttatggtccaactttacctatgaaccagacagatgatgcatcttttggagaattttgtcgtgatacagtatcaacaatttggcatttccacggcggatgccttgtcggaaaggtggtcgatagcagcttgcgggtcatgggaatcaatgctttgcgtgttgttgacgcatccacattcaatttcacaccggggacaaatcctcaggccacacttatgatgttgggcaggtacattggcattaagatgctgcaacaaagatcttcttaa

Protein Analysis

601

Amino Acids

64.75

Weight (kDa)

9.41

Isoelectric Point (pI)

32.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 332, 1755
AccB7I CCANNNNNTGG 1 cut(s) 752
AciI CCGC 5 cut(s) 792, 1050, 1402, 1632, 1669
AclWI GGATC 2 cut(s) 774, 1271
AcoI YGGCCR 1 cut(s) 228
AcsI RAATTY 4 cut(s) 593, 876, 1300, 1589
AcuI CTGAAG 1 cut(s) 528
AfaI GTAC 3 cut(s) 984, 1354, 1769
AfiI CCNNNNNNNGG 3 cut(s) 155, 752, 1115
Alw21I GWGCWC 2 cut(s) 409, 1523
Alw26I GTCTC 4 cut(s) 88, 676, 972, 1220
AlwI GGATC 2 cut(s) 774, 1271
Ama87I CYCGRG 1 cut(s) 933
AoxI GGCC 4 cut(s) 34, 96, 228, 1743
ApeKI GCWGC 3 cut(s) 356, 1662, 1786
ApoI RAATTY 4 cut(s) 593, 876, 1300, 1589
Asp700I GAANNNNTTC 1 cut(s) 444
AspS9I GGNCC 9 cut(s) 96, 161, 210, 743, 1058, 1337, 1391, 1429, 1544
AsuC2I CCSGG 2 cut(s) 208, 1728
AsuHPI GGTGA 2 cut(s) 196, 1091
AvaI CYCGRG 1 cut(s) 933
AvaII GGWCC 8 cut(s) 161, 210, 743, 1058, 1337, 1391, 1429, 1544
AxyI CCTNAGG 1 cut(s) 1740
BalI TGGCCA 1 cut(s) 230
BbsI GAAGAC 1 cut(s) 516
Bbv12I GWGCWC 2 cut(s) 409, 1523
BbvCI CCTCAGC 1 cut(s) 1032
BbvI GCAGC 3 cut(s) 368, 1674, 1773
BccI CCATC 3 cut(s) 172, 467, 1331
BceAI ACGGC 2 cut(s) 41, 1645
BcgI CGANNNNNNTGC 2 cut(s) 360, 394
BclI TGATCA 1 cut(s) 558
BcnI CCSGG 2 cut(s) 208, 1728
BcoDI GTCTC 4 cut(s) 88, 676, 972, 1220
BfaI CTAG 4 cut(s) 14, 32, 182, 1236
BfmI CTRYAG 3 cut(s) 192, 1175, 1261
BfuAI ACCTGC 2 cut(s) 332, 1755
BglII AGATCT 1 cut(s) 1796
BisI GCNGC 3 cut(s) 357, 1663, 1787
BlsI GCNGC 3 cut(s) 358, 1664, 1788
Bme1390I CCNGG 2 cut(s) 208, 1728
Bme18I GGWCC 8 cut(s) 161, 210, 743, 1058, 1337, 1391, 1429, 1544
BmeT110I CYCGRG 1 cut(s) 933
BmgT120I GGNCC 9 cut(s) 96, 161, 210, 743, 1058, 1337, 1391, 1429, 1544
BmiI GGNNCC 3 cut(s) 97, 147, 211
BmrFI CCNGG 2 cut(s) 208, 1728
BmrI ACTGGG 2 cut(s) 661, 1085
BmsI GCATC 7 cut(s) 245, 271, 1564, 1586, 1625, 1715, 1773
BmuI ACTGGG 2 cut(s) 661, 1085
BoxI GACNNNNGTC 1 cut(s) 1342
BpiI GAAGAC 1 cut(s) 516
BplI GAGNNNNNCTC 2 cut(s) 1217, 1249
Bpu10I CCTNAGC 1 cut(s) 1032
BpuEI CTTGAG 1 cut(s) 535
BpuMI CCSGG 2 cut(s) 208, 1728
BsaAI YACGTR 1 cut(s) 986
BsaI GGTCTC 2 cut(s) 972, 1220
BsaJI CCNNGG 4 cut(s) 57, 1425, 1627, 1727
Bsc4I CCNNNNNNNGG 3 cut(s) 155, 752, 1115
Bse1I ACTGG 3 cut(s) 656, 1091, 1120
Bse21I CCTNAGG 1 cut(s) 1740
Bse3DI GCAATG 1 cut(s) 1317
BseDI CCNNGG 4 cut(s) 57, 1425, 1627, 1727
BseGI GGATG 5 cut(s) 286, 418, 1420, 1640, 1706
BseLI CCNNNNNNNGG 3 cut(s) 155, 752, 1115
BseMI GCAATG 1 cut(s) 1317
BseMII CTCAG 6 cut(s) 99, 440, 998, 1046, 1076, 1754
BseNI ACTGG 3 cut(s) 656, 1091, 1120
BseXI GCAGC 3 cut(s) 368, 1674, 1773
BsgI GTGCAG 4 cut(s) 153, 225, 399, 1032
BshFI GGCC 4 cut(s) 36, 98, 230, 1745
BsiHKAI GWGCWC 2 cut(s) 409, 1523
BsiHKCI CYCGRG 1 cut(s) 933
BsiSI CCGG 3 cut(s) 207, 1280, 1727
BslFI GGGAC 4 cut(s) 223, 635, 1147, 1744
BslI CCNNNNNNNGG 3 cut(s) 155, 752, 1115
BsmAI GTCTC 4 cut(s) 88, 676, 972, 1220
BsmBI CGTCTC 1 cut(s) 676
BsmFI GGGAC 4 cut(s) 223, 635, 1147, 1744
BsnI GGCC 4 cut(s) 36, 98, 230, 1745
Bso31I GGTCTC 2 cut(s) 972, 1220
BsoBI CYCGRG 1 cut(s) 933
Bsp1286I GDGCHC 2 cut(s) 409, 1523
Bsp143I GATC 6 cut(s) 289, 480, 558, 766, 1276, 1796
BspACI CCGC 5 cut(s) 792, 1050, 1402, 1632, 1669
BspANI GGCC 4 cut(s) 36, 98, 230, 1745
BspCNI CTCAG 6 cut(s) 98, 441, 999, 1045, 1075, 1753
BspHI TCATGA 1 cut(s) 250
BspLI GGNNCC 3 cut(s) 97, 147, 211
BspMI ACCTGC 2 cut(s) 332, 1755
BspPI GGATC 2 cut(s) 774, 1271
BspTNI GGTCTC 2 cut(s) 972, 1220
BsrDI GCAATG 1 cut(s) 1317
BsrI ACTGG 3 cut(s) 656, 1091, 1120
BssECI CCNNGG 4 cut(s) 57, 1425, 1627, 1727
BssMI GATC 6 cut(s) 289, 480, 558, 766, 1276, 1796
BssT1I CCWWGG 1 cut(s) 1425
Bst4CI ACNGT 7 cut(s) 297, 515, 667, 889, 1189, 1265, 1606
Bst6I CTCTTC 1 cut(s) 913
BstBAI YACGTR 1 cut(s) 986
BstC8I GCNNGC 3 cut(s) 373, 1241, 1667
BstDEI CTNAG 8 cut(s) 74, 85, 449, 1007, 1032, 1046, 1062, 1740
BstDSI CCRYGG 2 cut(s) 57, 1627
BstF5I GGATG 5 cut(s) 286, 418, 1420, 1640, 1706
BstKTI GATC 6 cut(s) 292, 483, 561, 769, 1279, 1799
BstMAI GTCTC 4 cut(s) 88, 676, 972, 1220
BstMBI GATC 6 cut(s) 289, 480, 558, 766, 1276, 1796
BstMWI GCNNNNNNNGC 2 cut(s) 62, 413
BstNSI RCATGY 1 cut(s) 1243
BstPAI GACNNNNGTC 1 cut(s) 1342
BstSCI CCNGG 2 cut(s) 206, 1726
BstSFI CTRYAG 3 cut(s) 192, 1175, 1261
BstV1I GCAGC 3 cut(s) 368, 1674, 1773
BstV2I GAAGAC 1 cut(s) 516
BstX2I RGATCY 2 cut(s) 1276, 1796
BstXI CCANNNNNNTGG 1 cut(s) 158
BstYI RGATCY 2 cut(s) 1276, 1796
Bsu36I CCTNAGG 1 cut(s) 1740
BsuRI GGCC 4 cut(s) 36, 98, 230, 1745
BtgI CCRYGG 2 cut(s) 57, 1627
BtsCI GGATG 5 cut(s) 286, 418, 1420, 1640, 1706
BtsIMutI CAGTG 6 cut(s) 285, 649, 663, 1113, 1270, 1487
BveI ACCTGC 2 cut(s) 332, 1755
Cac8I GCNNGC 3 cut(s) 373, 1241, 1667
CciI TCATGA 1 cut(s) 250
Cfr13I GGNCC 9 cut(s) 96, 161, 210, 743, 1058, 1337, 1391, 1429, 1544
CseI GACGC 1 cut(s) 1712
Csp6I GTAC 3 cut(s) 983, 1353, 1768
CviQI GTAC 3 cut(s) 983, 1353, 1768
DdeI CTNAG 8 cut(s) 74, 85, 449, 1007, 1032, 1046, 1062, 1740
DpnI GATC 6 cut(s) 291, 482, 560, 768, 1278, 1798
DpnII GATC 6 cut(s) 289, 480, 558, 766, 1276, 1796
EaeI YGGCCR 1 cut(s) 228
Eam1104I CTCTTC 1 cut(s) 913
EarI CTCTTC 1 cut(s) 913
EciI GGCGGA 1 cut(s) 1647
Eco130I CCWWGG 1 cut(s) 1425
Eco147I AGGCCT 1 cut(s) 36
Eco31I GGTCTC 2 cut(s) 972, 1220
Eco47I GGWCC 8 cut(s) 161, 210, 743, 1058, 1337, 1391, 1429, 1544
Eco57I CTGAAG 1 cut(s) 528
Eco81I CCTNAGG 1 cut(s) 1740
Eco88I CYCGRG 1 cut(s) 933
EcoO109I RGGNCCY 2 cut(s) 210, 1337
EcoRI GAATTC 2 cut(s) 593, 876
EcoT14I CCWWGG 1 cut(s) 1425
EcoT22I ATGCAT 2 cut(s) 238, 1579
ErhI CCWWGG 1 cut(s) 1425
Esp3I CGTCTC 1 cut(s) 676
FalI AAGNNNNNCTT 1 cut(s) 1786
FaqI GGGAC 4 cut(s) 223, 635, 1147, 1744
FauI CCCGC 1 cut(s) 1662
FbaI TGATCA 1 cut(s) 558
Fnu4HI GCNGC 3 cut(s) 357, 1663, 1787
FokI GGATG 5 cut(s) 293, 405, 1407, 1647, 1693
Fsp4HI GCNGC 3 cut(s) 357, 1663, 1787
FspBI CTAG 4 cut(s) 14, 32, 182, 1236
GluI GCNGC 3 cut(s) 357, 1663, 1787
HaeIII GGCC 4 cut(s) 36, 98, 230, 1745
HapII CCGG 3 cut(s) 207, 1280, 1727
HgaI GACGC 1 cut(s) 1712
HincII GTYRAC 1 cut(s) 1702
HindII GTYRAC 1 cut(s) 1702
HindIII AAGCTT 3 cut(s) 1361, 1509, 1527
HinfI GANTC 6 cut(s) 756, 917, 953, 1027, 1314, 1680
HpaII CCGG 3 cut(s) 207, 1280, 1727
HphI GGTGA 2 cut(s) 196, 1091
Hpy166II GTNNAC 4 cut(s) 119, 161, 1099, 1702
Hpy188I TCNGA 8 cut(s) 24, 88, 450, 508, 530, 1008, 1378, 1646
Hpy188III TCNNGA 6 cut(s) 251, 392, 609, 892, 1274, 1598
Hpy8I GTNNAC 4 cut(s) 119, 161, 1099, 1702
Hpy99I CGWCG 1 cut(s) 1519
HpyAV CCTTC 5 cut(s) 86, 271, 434, 528, 810
HpyCH4III ACNGT 7 cut(s) 297, 515, 667, 889, 1189, 1265, 1606
HpyCH4IV ACGT 2 cut(s) 691, 985
HpyCH4V TGCA 9 cut(s) 170, 236, 242, 416, 463, 1013, 1418, 1577, 1789
HpyF10VI GCNNNNNNNGC 2 cut(s) 62, 413
HpyF3I CTNAG 8 cut(s) 74, 85, 449, 1007, 1032, 1046, 1062, 1740
HpySE526I ACGT 2 cut(s) 691, 985
Ksp22I TGATCA 1 cut(s) 558
Kzo9I GATC 6 cut(s) 289, 480, 558, 766, 1276, 1796
LmnI GCTCC 3 cut(s) 151, 412, 827
Lsp1109I GCAGC 3 cut(s) 368, 1674, 1773
LweI GCATC 7 cut(s) 245, 271, 1564, 1586, 1625, 1715, 1773
MaeI CTAG 4 cut(s) 14, 32, 182, 1236
MaeII ACGT 2 cut(s) 691, 985
MaeIII GTNAC 2 cut(s) 687, 1485
MalI GATC 6 cut(s) 291, 482, 560, 768, 1278, 1798
MboI GATC 6 cut(s) 289, 480, 558, 766, 1276, 1796
MfeI CAATTG 1 cut(s) 1020
MflI RGATCY 2 cut(s) 1276, 1796
MhlI GDGCHC 2 cut(s) 409, 1523
MlsI TGGCCA 1 cut(s) 230
MluNI TGGCCA 1 cut(s) 230
MlyI GAGTC 2 cut(s) 1036, 1308
MmeI TCCRAC 4 cut(s) 279, 708, 1571, 1624
Mox20I TGGCCA 1 cut(s) 230
Mph1103I ATGCAT 2 cut(s) 238, 1579
MroXI GAANNNNTTC 1 cut(s) 444
MscI TGGCCA 1 cut(s) 230
MslI CAYNNNNRTG 3 cut(s) 423, 1751, 1781
Msp20I TGGCCA 1 cut(s) 230
MspI CCGG 3 cut(s) 207, 1280, 1727
MspR9I CCNGG 2 cut(s) 208, 1728
MunI CAATTG 1 cut(s) 1020
MwoI GCNNNNNNNGC 2 cut(s) 62, 413
NciI CCSGG 2 cut(s) 208, 1728
NdeII GATC 6 cut(s) 289, 480, 558, 766, 1276, 1796
NlaIV GGNNCC 3 cut(s) 97, 147, 211
NmuCI GTSAC 2 cut(s) 687, 1485
NsiI ATGCAT 2 cut(s) 238, 1579
NspI RCATGY 1 cut(s) 1243
PaeI GCATGC 1 cut(s) 1243
PaeR7I CTCGAG 1 cut(s) 933
PagI TCATGA 1 cut(s) 250
PceI AGGCCT 1 cut(s) 36
PcsI WCGNNNNNNNCGW 1 cut(s) 1206
PdmI GAANNNNTTC 1 cut(s) 444
PfeI GAWTC 4 cut(s) 756, 917, 953, 1680
PflMI CCANNNNNTGG 1 cut(s) 752
PkrI GCNGC 3 cut(s) 358, 1664, 1788
PleI GAGTC 2 cut(s) 1035, 1308
PpsI GAGTC 2 cut(s) 1035, 1308
Ppu21I YACGTR 1 cut(s) 986
PpuMI RGGWCCY 2 cut(s) 210, 1337
PshAI GACNNNNGTC 1 cut(s) 1342
Psp5II RGGWCCY 2 cut(s) 210, 1337
PspN4I GGNNCC 3 cut(s) 97, 147, 211
PspPI GGNCC 9 cut(s) 96, 161, 210, 743, 1058, 1337, 1391, 1429, 1544
PspPPI RGGWCCY 2 cut(s) 210, 1337
PspXI VCTCGAGB 1 cut(s) 933
PsuI RGATCY 2 cut(s) 1276, 1796
RsaI GTAC 3 cut(s) 984, 1354, 1769
RsaNI GTAC 3 cut(s) 983, 1353, 1768
RseI CAYNNNNRTG 3 cut(s) 423, 1751, 1781
SatI GCNGC 3 cut(s) 357, 1663, 1787
Sau3AI GATC 6 cut(s) 289, 480, 558, 766, 1276, 1796
Sau96I GGNCC 9 cut(s) 96, 161, 210, 743, 1058, 1337, 1391, 1429, 1544
SchI GAGTC 2 cut(s) 1036, 1308
ScrFI CCNGG 2 cut(s) 208, 1728
SduI GDGCHC 2 cut(s) 409, 1523
SfaNI GCATC 7 cut(s) 245, 271, 1564, 1586, 1625, 1715, 1773
SfcI CTRYAG 3 cut(s) 192, 1175, 1261
Sfr274I CTCGAG 1 cut(s) 933
SinI GGWCC 8 cut(s) 161, 210, 743, 1058, 1337, 1391, 1429, 1544
SlaI CTCGAG 1 cut(s) 933
SmiMI CAYNNNNRTG 3 cut(s) 423, 1751, 1781
SmlI CTYRAG 2 cut(s) 550, 933
SmoI CTYRAG 2 cut(s) 550, 933
SphI GCATGC 1 cut(s) 1243
SseBI AGGCCT 1 cut(s) 36
SsiI CCGC 5 cut(s) 792, 1050, 1402, 1632, 1669
SspMI CTAG 4 cut(s) 14, 32, 182, 1236
StuI AGGCCT 1 cut(s) 36
StyD4I CCNGG 2 cut(s) 206, 1726
StyI CCWWGG 1 cut(s) 1425
TaaI ACNGT 7 cut(s) 297, 515, 667, 889, 1189, 1265, 1606
TaiI ACGT 2 cut(s) 694, 988
TaqI TCGA 4 cut(s) 891, 934, 1517, 1656
TfiI GAWTC 4 cut(s) 756, 917, 953, 1680
TscAI CASTG 6 cut(s) 292, 656, 670, 1120, 1270, 1494
TseFI GTSAC 2 cut(s) 687, 1485
TseI GCWGC 3 cut(s) 356, 1662, 1786
Tsp45I GTSAC 2 cut(s) 687, 1485
TspDTI ATGAA 8 cut(s) 143, 209, 213, 281, 869, 1138, 1469, 1574
TspGWI ACGGA 1 cut(s) 840
TspRI CASTG 6 cut(s) 292, 656, 670, 1120, 1270, 1494
Van91I CCANNNNNTGG 1 cut(s) 752
VpaK11BI GGWCC 8 cut(s) 161, 210, 743, 1058, 1337, 1391, 1429, 1544
XapI RAATTY 4 cut(s) 593, 876, 1300, 1589
XceI RCATGY 1 cut(s) 1243
XcmI CCANNNNNNNNNTGG 1 cut(s) 1112
XhoI CTCGAG 1 cut(s) 933
XmnI GAANNNNTTC 1 cut(s) 444
XspI CTAG 4 cut(s) 14, 32, 182, 1236
Zsp2I ATGCAT 2 cut(s) 238, 1579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.