(R)-mandelonitrile lyase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 1136 |
| AatII | GACGTC | 1 cut(s) | 1134 |
| Acc36I | ACCTGC | 1 cut(s) | 1509 |
| AccB1I | GGYRCC | 1 cut(s) | 105 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 503, 1368 |
| AciI | CCGC | 4 cut(s) | 129, 303, 636, 1298 |
| AclWI | GGATC | 3 cut(s) | 656, 1439, 1465 |
| AcsI | RAATTY | 5 cut(s) | 214, 350, 439, 1051, 1154 |
| AcuI | CTGAAG | 1 cut(s) | 705 |
| AcyI | GRCGYC | 1 cut(s) | 1131 |
| AfaI | GTAC | 2 cut(s) | 41, 547 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 65, 157, 503, 714, 866, 1368, 1426 |
| AflIII | ACRYGT | 2 cut(s) | 186, 1444 |
| AgsI | TTSAA | 7 cut(s) | 416, 1056, 1159, 1243, 1337, 1468, 1541 |
| AjiI | CACGTC | 1 cut(s) | 261 |
| AjnI | CCWGG | 2 cut(s) | 1267, 1480 |
| AleI | CACNNNNGTG | 1 cut(s) | 425 |
| AluBI | AGCT | 3 cut(s) | 278, 485, 683 |
| AluI | AGCT | 3 cut(s) | 278, 485, 683 |
| Alw21I | GWGCWC | 2 cut(s) | 147, 522 |
| Alw26I | GTCTC | 2 cut(s) | 723, 971 |
| AlwI | GGATC | 3 cut(s) | 656, 1439, 1465 |
| Ama87I | CYCGRG | 1 cut(s) | 1257 |
| AoxI | GGCC | 3 cut(s) | 246, 808, 1008 |
| ApeKI | GCWGC | 2 cut(s) | 20, 116 |
| ApoI | RAATTY | 5 cut(s) | 214, 350, 439, 1051, 1154 |
| AspA2I | CCTAGG | 1 cut(s) | 298 |
| AspS9I | GGNCC | 5 cut(s) | 247, 295, 809, 1295, 1485 |
| AsuC2I | CCSGG | 1 cut(s) | 1421 |
| AsuHPI | GGTGA | 4 cut(s) | 758, 842, 1229, 1470 |
| AvaI | CYCGRG | 1 cut(s) | 1257 |
| AvaII | GGWCC | 3 cut(s) | 295, 1295, 1485 |
| AvrII | CCTAGG | 1 cut(s) | 298 |
| BaeGI | GKGCMC | 1 cut(s) | 110 |
| BanI | GGYRCC | 1 cut(s) | 105 |
| BbsI | GAAGAC | 2 cut(s) | 43, 577 |
| Bbv12I | GWGCWC | 2 cut(s) | 147, 522 |
| BbvI | GCAGC | 2 cut(s) | 7, 128 |
| BccI | CCATC | 5 cut(s) | 263, 925, 1271, 1403, 1448 |
| BceAI | ACGGC | 1 cut(s) | 1187 |
| BciT130I | CCWGG | 2 cut(s) | 1269, 1482 |
| BclI | TGATCA | 1 cut(s) | 315 |
| BcnI | CCSGG | 1 cut(s) | 1421 |
| BcoDI | GTCTC | 2 cut(s) | 723, 971 |
| BfaI | CTAG | 5 cut(s) | 24, 152, 299, 425, 926 |
| BfuAI | ACCTGC | 1 cut(s) | 1509 |
| BisI | GCNGC | 3 cut(s) | 21, 117, 636 |
| BlnI | CCTAGG | 1 cut(s) | 298 |
| BlsI | GCNGC | 3 cut(s) | 22, 118, 637 |
| Bme1390I | CCNGG | 3 cut(s) | 1269, 1421, 1482 |
| Bme18I | GGWCC | 3 cut(s) | 295, 1295, 1485 |
| BmeT110I | CYCGRG | 1 cut(s) | 1257 |
| BmgBI | CACGTC | 1 cut(s) | 261 |
| BmgT120I | GGNCC | 5 cut(s) | 247, 295, 809, 1295, 1485 |
| BmiI | GGNNCC | 3 cut(s) | 107, 296, 1486 |
| BmrFI | CCNGG | 3 cut(s) | 1269, 1421, 1482 |
| BmrI | ACTGGG | 1 cut(s) | 836 |
| BmsI | GCATC | 4 cut(s) | 460, 677, 1337, 1376 |
| BmuI | ACTGGG | 1 cut(s) | 836 |
| BpiI | GAAGAC | 2 cut(s) | 43, 577 |
| BpuEI | CTTGAG | 3 cut(s) | 319, 341, 1479 |
| BpuMI | CCSGG | 1 cut(s) | 1421 |
| BsaAI | YACGTR | 2 cut(s) | 737, 1445 |
| BsaHI | GRCGYC | 1 cut(s) | 1131 |
| BsaI | GGTCTC | 1 cut(s) | 723 |
| BsaJI | CCNNGG | 5 cut(s) | 100, 298, 562, 1378, 1481 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 65, 157, 503, 714, 866, 1368, 1426 |
| Bse118I | RCCGGY | 1 cut(s) | 244 |
| Bse1I | ACTGG | 3 cut(s) | 455, 842, 1315 |
| BseBI | CCWGG | 2 cut(s) | 1269, 1482 |
| BseDI | CCNNGG | 5 cut(s) | 100, 298, 562, 1378, 1481 |
| BseGI | GGATG | 5 cut(s) | 96, 604, 1121, 1328, 1391 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 65, 157, 503, 714, 866, 1368, 1426 |
| BseMII | CTCAG | 1 cut(s) | 608 |
| BseNI | ACTGG | 3 cut(s) | 455, 842, 1315 |
| BseRI | GAGGAG | 1 cut(s) | 137 |
| BseSI | GKGCMC | 1 cut(s) | 110 |
| BseXI | GCAGC | 2 cut(s) | 7, 128 |
| BseYI | CCCAGC | 1 cut(s) | 200 |
| BsgI | GTGCAG | 2 cut(s) | 783, 924 |
| Bsh1285I | CGRYCG | 1 cut(s) | 562 |
| BshFI | GGCC | 3 cut(s) | 248, 810, 1010 |
| BshNI | GGYRCC | 1 cut(s) | 105 |
| BsiEI | CGRYCG | 1 cut(s) | 562 |
| BsiHKAI | GWGCWC | 2 cut(s) | 147, 522 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1257 |
| BsiSI | CCGG | 3 cut(s) | 245, 987, 1420 |
| BslFI | GGGAC | 2 cut(s) | 781, 1498 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 65, 157, 503, 714, 866, 1368, 1426 |
| BsmAI | GTCTC | 2 cut(s) | 723, 971 |
| BsmFI | GGGAC | 2 cut(s) | 781, 1498 |
| BsmI | GAATGC | 1 cut(s) | 1536 |
| BsnI | GGCC | 3 cut(s) | 248, 810, 1010 |
| Bso31I | GGTCTC | 1 cut(s) | 723 |
| BsoBI | CYCGRG | 1 cut(s) | 1257 |
| Bsp1286I | GDGCHC | 3 cut(s) | 110, 147, 522 |
| Bsp143I | GATC | 5 cut(s) | 315, 549, 648, 1431, 1457 |
| Bsp19I | CCATGG | 1 cut(s) | 1378 |
| BspACI | CCGC | 4 cut(s) | 129, 303, 636, 1298 |
| BspANI | GGCC | 3 cut(s) | 248, 810, 1010 |
| BspCNI | CTCAG | 1 cut(s) | 609 |
| BspLI | GGNNCC | 3 cut(s) | 107, 296, 1486 |
| BspMI | ACCTGC | 1 cut(s) | 1509 |
| BspPI | GGATC | 3 cut(s) | 656, 1439, 1465 |
| BspT107I | GGYRCC | 1 cut(s) | 105 |
| BspTNI | GGTCTC | 1 cut(s) | 723 |
| BsrFI | RCCGGY | 1 cut(s) | 244 |
| BsrI | ACTGG | 3 cut(s) | 455, 842, 1315 |
| BssAI | RCCGGY | 1 cut(s) | 244 |
| BssECI | CCNNGG | 5 cut(s) | 100, 298, 562, 1378, 1481 |
| BssMI | GATC | 5 cut(s) | 315, 549, 648, 1431, 1457 |
| BssNI | GRCGYC | 1 cut(s) | 1131 |
| BssT1I | CCWWGG | 3 cut(s) | 100, 298, 1378 |
| Bst2UI | CCWGG | 2 cut(s) | 1269, 1482 |
| Bst4CI | ACNGT | 5 cut(s) | 236, 347, 563, 982, 1357 |
| Bst6I | CTCTTC | 1 cut(s) | 664 |
| BstACI | GRCGYC | 1 cut(s) | 1131 |
| BstAPI | GCANNNNNTGC | 4 cut(s) | 20, 632, 877, 947 |
| BstBAI | YACGTR | 2 cut(s) | 737, 1445 |
| BstC8I | GCNNGC | 1 cut(s) | 992 |
| BstDEI | CTNAG | 3 cut(s) | 388, 617, 797 |
| BstDSI | CCRYGG | 2 cut(s) | 562, 1378 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 63, 712 |
| BstF5I | GGATG | 5 cut(s) | 96, 604, 1121, 1328, 1391 |
| BstKTI | GATC | 5 cut(s) | 318, 552, 651, 1434, 1460 |
| BstMAI | GTCTC | 2 cut(s) | 723, 971 |
| BstMBI | GATC | 5 cut(s) | 315, 549, 648, 1431, 1457 |
| BstMCI | CGRYCG | 1 cut(s) | 562 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 20, 632, 877, 947, 1175 |
| BstNI | CCWGG | 2 cut(s) | 1269, 1482 |
| BstNSI | RCATGY | 1 cut(s) | 1530 |
| BstSCI | CCNGG | 3 cut(s) | 1267, 1419, 1480 |
| BstSLI | GKGCMC | 1 cut(s) | 110 |
| BstV1I | GCAGC | 2 cut(s) | 7, 128 |
| BstV2I | GAAGAC | 2 cut(s) | 43, 577 |
| BstX2I | RGATCY | 1 cut(s) | 1457 |
| BstYI | RGATCY | 1 cut(s) | 1457 |
| BsuRI | GGCC | 3 cut(s) | 248, 810, 1010 |
| BtgI | CCRYGG | 2 cut(s) | 562, 1378 |
| BtrI | CACGTC | 1 cut(s) | 261 |
| BtsCI | GGATG | 5 cut(s) | 96, 604, 1121, 1328, 1391 |
| BtsIMutI | CAGTG | 3 cut(s) | 352, 448, 699 |
| BveI | ACCTGC | 1 cut(s) | 1509 |
| Cac8I | GCNNGC | 1 cut(s) | 992 |
| Cfr10I | RCCGGY | 1 cut(s) | 244 |
| Cfr13I | GGNCC | 5 cut(s) | 247, 295, 809, 1295, 1485 |
| Csp6I | GTAC | 2 cut(s) | 40, 546 |
| CviAII | CATG | 8 cut(s) | 220, 382, 575, 632, 1042, 1379, 1527, 1533 |
| CviQI | GTAC | 2 cut(s) | 40, 546 |
| DdeI | CTNAG | 3 cut(s) | 388, 617, 797 |
| DpnI | GATC | 5 cut(s) | 317, 551, 650, 1433, 1459 |
| DpnII | GATC | 5 cut(s) | 315, 549, 648, 1431, 1457 |
| DraI | TTTAAA | 1 cut(s) | 1252 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1136 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1136 |
| Eam1104I | CTCTTC | 1 cut(s) | 664 |
| EarI | CTCTTC | 1 cut(s) | 664 |
| Eco130I | CCWWGG | 3 cut(s) | 100, 298, 1378 |
| Eco31I | GGTCTC | 1 cut(s) | 723 |
| Eco47I | GGWCC | 3 cut(s) | 295, 1295, 1485 |
| Eco57I | CTGAAG | 1 cut(s) | 705 |
| Eco88I | CYCGRG | 1 cut(s) | 1257 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 63, 712 |
| EcoO109I | RGGNCCY | 1 cut(s) | 295 |
| EcoRI | GAATTC | 1 cut(s) | 350 |
| EcoRII | CCWGG | 2 cut(s) | 1267, 1480 |
| EcoT14I | CCWWGG | 3 cut(s) | 100, 298, 1378 |
| EcoT22I | ATGCAT | 3 cut(s) | 475, 1528, 1538 |
| ErhI | CCWWGG | 3 cut(s) | 100, 298, 1378 |
| FaeI | CATG | 8 cut(s) | 223, 385, 578, 635, 1045, 1382, 1530, 1536 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1488, 1520 |
| FaqI | GGGAC | 2 cut(s) | 781, 1498 |
| FatI | CATG | 8 cut(s) | 219, 381, 574, 631, 1041, 1378, 1526, 1532 |
| FauI | CCCGC | 1 cut(s) | 296 |
| FauNDI | CATATG | 1 cut(s) | 403 |
| FbaI | TGATCA | 1 cut(s) | 315 |
| Fnu4HI | GCNGC | 3 cut(s) | 21, 117, 636 |
| FokI | GGATG | 5 cut(s) | 83, 591, 1108, 1315, 1398 |
| Fsp4HI | GCNGC | 3 cut(s) | 21, 117, 636 |
| FspBI | CTAG | 5 cut(s) | 24, 152, 299, 425, 926 |
| GluI | GCNGC | 3 cut(s) | 21, 117, 636 |
| GsaI | CCCAGC | 1 cut(s) | 204 |
| HaeIII | GGCC | 3 cut(s) | 248, 810, 1010 |
| HapII | CCGG | 3 cut(s) | 245, 987, 1420 |
| Hin1I | GRCGYC | 1 cut(s) | 1131 |
| Hin1II | CATG | 8 cut(s) | 223, 385, 578, 635, 1045, 1382, 1530, 1536 |
| HinfI | GANTC | 4 cut(s) | 205, 690, 704, 778 |
| HpaII | CCGG | 3 cut(s) | 245, 987, 1420 |
| HphI | GGTGA | 4 cut(s) | 758, 842, 1229, 1470 |
| Hpy166II | GTNNAC | 4 cut(s) | 73, 258, 566, 850 |
| Hpy188I | TCNGA | 5 cut(s) | 265, 438, 922, 1321, 1397 |
| Hpy188III | TCNNGA | 6 cut(s) | 336, 358, 366, 497, 1259, 1349 |
| Hpy8I | GTNNAC | 4 cut(s) | 73, 258, 566, 850 |
| HpyAV | CCTTC | 4 cut(s) | 99, 680, 942, 1168 |
| HpyCH4III | ACNGT | 5 cut(s) | 236, 347, 563, 982, 1357 |
| HpyCH4IV | ACGT | 7 cut(s) | 186, 260, 736, 864, 900, 1131, 1444 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 20, 632, 877, 947, 1175 |
| HpyF3I | CTNAG | 3 cut(s) | 388, 617, 797 |
| HpySE526I | ACGT | 7 cut(s) | 186, 260, 736, 864, 900, 1131, 1444 |
| Hsp92I | GRCGYC | 1 cut(s) | 1131 |
| Hsp92II | CATG | 8 cut(s) | 223, 385, 578, 635, 1045, 1382, 1530, 1536 |
| Ksp22I | TGATCA | 1 cut(s) | 315 |
| Kzo9I | GATC | 5 cut(s) | 315, 549, 648, 1431, 1457 |
| LmnI | GCTCC | 2 cut(s) | 150, 517 |
| Lsp1109I | GCAGC | 2 cut(s) | 7, 128 |
| LweI | GCATC | 4 cut(s) | 460, 677, 1337, 1376 |
| MaeI | CTAG | 5 cut(s) | 24, 152, 299, 425, 926 |
| MaeII | ACGT | 7 cut(s) | 186, 260, 736, 864, 900, 1131, 1444 |
| MaeIII | GTNAC | 4 cut(s) | 194, 881, 901, 1072 |
| MalI | GATC | 5 cut(s) | 317, 551, 650, 1433, 1459 |
| MboI | GATC | 5 cut(s) | 315, 549, 648, 1431, 1457 |
| MboII | GAAGA | 6 cut(s) | 43, 239, 278, 582, 651, 1286 |
| MfeI | CAATTG | 2 cut(s) | 15, 771 |
| MflI | RGATCY | 1 cut(s) | 1457 |
| MhlI | GDGCHC | 3 cut(s) | 110, 147, 522 |
| MlyI | GAGTC | 1 cut(s) | 787 |
| MmeI | TCCRAC | 2 cut(s) | 1055, 1375 |
| Mph1103I | ATGCAT | 3 cut(s) | 475, 1528, 1538 |
| MseI | TTAA | 6 cut(s) | 372, 834, 1110, 1226, 1251, 1284 |
| MslI | CAYNNNNRTG | 5 cut(s) | 406, 425, 1377, 1505, 1531 |
| MspA1I | CMGCKG | 1 cut(s) | 683 |
| MspI | CCGG | 3 cut(s) | 245, 987, 1420 |
| MspR9I | CCNGG | 3 cut(s) | 1269, 1421, 1482 |
| MunI | CAATTG | 2 cut(s) | 15, 771 |
| Mva1269I | GAATGC | 1 cut(s) | 1536 |
| MvaI | CCWGG | 2 cut(s) | 1269, 1482 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 20, 632, 877, 947, 1175 |
| NciI | CCSGG | 1 cut(s) | 1421 |
| NcoI | CCATGG | 1 cut(s) | 1378 |
| NdeI | CATATG | 1 cut(s) | 403 |
| NdeII | GATC | 5 cut(s) | 315, 549, 648, 1431, 1457 |
| NlaIII | CATG | 8 cut(s) | 223, 385, 578, 635, 1045, 1382, 1530, 1536 |
| NlaIV | GGNNCC | 3 cut(s) | 107, 296, 1486 |
| NmuCI | GTSAC | 1 cut(s) | 881 |
| NsiI | ATGCAT | 3 cut(s) | 475, 1528, 1538 |
| NspI | RCATGY | 1 cut(s) | 1530 |
| OliI | CACNNNNGTG | 1 cut(s) | 425 |
| PaeR7I | CTCGAG | 1 cut(s) | 1257 |
| PctI | GAATGC | 1 cut(s) | 1536 |
| PfeI | GAWTC | 3 cut(s) | 205, 690, 704 |
| PflFI | GACNNNGTC | 1 cut(s) | 982 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 503, 1368 |
| PkrI | GCNGC | 3 cut(s) | 22, 118, 637 |
| PleI | GAGTC | 1 cut(s) | 786 |
| PpsI | GAGTC | 1 cut(s) | 786 |
| Ppu21I | YACGTR | 2 cut(s) | 737, 1445 |
| PpuMI | RGGWCCY | 1 cut(s) | 295 |
| Psp5II | RGGWCCY | 1 cut(s) | 295 |
| Psp6I | CCWGG | 2 cut(s) | 1267, 1480 |
| PspFI | CCCAGC | 1 cut(s) | 200 |
| PspGI | CCWGG | 2 cut(s) | 1267, 1480 |
| PspN4I | GGNNCC | 3 cut(s) | 107, 296, 1486 |
| PspPI | GGNCC | 5 cut(s) | 247, 295, 809, 1295, 1485 |
| PspPPI | RGGWCCY | 1 cut(s) | 295 |
| PsuI | RGATCY | 1 cut(s) | 1457 |
| PsyI | GACNNNGTC | 1 cut(s) | 982 |
| PvuII | CAGCTG | 1 cut(s) | 683 |
| RsaI | GTAC | 2 cut(s) | 41, 547 |
| RsaNI | GTAC | 2 cut(s) | 40, 546 |
| RseI | CAYNNNNRTG | 5 cut(s) | 406, 425, 1377, 1505, 1531 |
| SaqAI | TTAA | 6 cut(s) | 372, 834, 1110, 1226, 1251, 1284 |
| SatI | GCNGC | 3 cut(s) | 21, 117, 636 |
| Sau3AI | GATC | 5 cut(s) | 315, 549, 648, 1431, 1457 |
| Sau96I | GGNCC | 5 cut(s) | 247, 295, 809, 1295, 1485 |
| SchI | GAGTC | 1 cut(s) | 787 |
| ScrFI | CCNGG | 3 cut(s) | 1269, 1421, 1482 |
| SduI | GDGCHC | 3 cut(s) | 110, 147, 522 |
| SfaNI | GCATC | 4 cut(s) | 460, 677, 1337, 1376 |
| Sfr274I | CTCGAG | 1 cut(s) | 1257 |
| SinI | GGWCC | 3 cut(s) | 295, 1295, 1485 |
| SlaI | CTCGAG | 1 cut(s) | 1257 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 406, 425, 1377, 1505, 1531 |
| SmlI | CTYRAG | 4 cut(s) | 334, 356, 1257, 1494 |
| SmoI | CTYRAG | 4 cut(s) | 334, 356, 1257, 1494 |
| SsiI | CCGC | 4 cut(s) | 129, 303, 636, 1298 |
| SspI | AATATT | 1 cut(s) | 322 |
| SspMI | CTAG | 5 cut(s) | 24, 152, 299, 425, 926 |
| StyD4I | CCNGG | 3 cut(s) | 1267, 1419, 1480 |
| StyI | CCWWGG | 3 cut(s) | 100, 298, 1378 |
| TaaI | ACNGT | 5 cut(s) | 236, 347, 563, 982, 1357 |
| TaiI | ACGT | 7 cut(s) | 189, 263, 739, 867, 903, 1134, 1447 |
| TaqI | TCGA | 3 cut(s) | 1258, 1348, 1434 |
| TatI | WGTACW | 1 cut(s) | 39 |
| TauI | GCSGC | 1 cut(s) | 638 |
| TfiI | GAWTC | 3 cut(s) | 205, 690, 704 |
| Tru1I | TTAA | 6 cut(s) | 372, 834, 1110, 1226, 1251, 1284 |
| Tru9I | TTAA | 6 cut(s) | 372, 834, 1110, 1226, 1251, 1284 |
| TscAI | CASTG | 3 cut(s) | 352, 455, 706 |
| TseFI | GTSAC | 1 cut(s) | 881 |
| TseI | GCWGC | 2 cut(s) | 20, 116 |
| Tsp45I | GTSAC | 1 cut(s) | 881 |
| TspDTI | ATGAA | 8 cut(s) | 208, 390, 398, 420, 620, 816, 1325, 1521 |
| TspGWI | ACGGA | 2 cut(s) | 130, 422 |
| TspRI | CASTG | 3 cut(s) | 352, 455, 706 |
| Tth111I | GACNNNGTC | 1 cut(s) | 982 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 503, 1368 |
| VpaK11BI | GGWCC | 3 cut(s) | 295, 1295, 1485 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 63, 712 |
| XapI | RAATTY | 5 cut(s) | 214, 350, 439, 1051, 1154 |
| XceI | RCATGY | 1 cut(s) | 1530 |
| XhoI | CTCGAG | 1 cut(s) | 1257 |
| XmaJI | CCTAGG | 1 cut(s) | 298 |
| XspI | CTAG | 5 cut(s) | 24, 152, 299, 425, 926 |
| ZraI | GACGTC | 1 cut(s) | 1132 |
| Zsp2I | ATGCAT | 3 cut(s) | 475, 1528, 1538 |