(R)-mandelonitrile lyase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 515 |
| Acc36I | ACCTGC | 1 cut(s) | 1596 |
| AccIII | TCCGGA | 1 cut(s) | 353 |
| AciI | CCGC | 4 cut(s) | 393, 633, 1004, 1287 |
| AclWI | GGATC | 2 cut(s) | 134, 1529 |
| AcoI | YGGCCR | 1 cut(s) | 958 |
| AcsI | RAATTY | 3 cut(s) | 119, 1167, 1430 |
| AcuI | CTGAAG | 2 cut(s) | 44, 505 |
| AfaI | GTAC | 3 cut(s) | 227, 375, 825 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 956, 1458 |
| AflIII | ACRYGT | 1 cut(s) | 1534 |
| AgsI | TTSAA | 6 cut(s) | 341, 506, 607, 1246, 1257, 1375 |
| AjnI | CCWGG | 2 cut(s) | 570, 616 |
| AluBI | AGCT | 4 cut(s) | 111, 431, 671, 1343 |
| AluI | AGCT | 4 cut(s) | 111, 431, 671, 1343 |
| Alw21I | GWGCWC | 2 cut(s) | 390, 1345 |
| AlwI | GGATC | 2 cut(s) | 134, 1529 |
| Aor13HI | TCCGGA | 1 cut(s) | 353 |
| AoxI | GGCC | 3 cut(s) | 524, 899, 958 |
| ApeKI | GCWGC | 2 cut(s) | 206, 668 |
| ApoI | RAATTY | 3 cut(s) | 119, 1167, 1430 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 763 |
| AspLEI | GCGC | 1 cut(s) | 254 |
| AspS9I | GGNCC | 2 cut(s) | 899, 1175 |
| AsuHPI | GGTGA | 2 cut(s) | 622, 1220 |
| AsuII | TTCGAA | 1 cut(s) | 1425 |
| AvaII | GGWCC | 1 cut(s) | 1175 |
| AxyI | CCTNAGG | 1 cut(s) | 1313 |
| BalI | TGGCCA | 1 cut(s) | 960 |
| BanII | GRGCYC | 2 cut(s) | 872, 1345 |
| BauI | CACGAG | 1 cut(s) | 397 |
| BbsI | GAAGAC | 2 cut(s) | 511, 513 |
| Bbv12I | GWGCWC | 2 cut(s) | 390, 1345 |
| BbvI | GCAGC | 2 cut(s) | 218, 680 |
| BccI | CCATC | 3 cut(s) | 353, 1129, 1361 |
| BceAI | ACGGC | 1 cut(s) | 758 |
| BciT130I | CCWGG | 2 cut(s) | 572, 618 |
| BclI | TGATCA | 1 cut(s) | 405 |
| BfmI | CTRYAG | 3 cut(s) | 261, 285, 1446 |
| BfoI | RGCGCY | 1 cut(s) | 255 |
| BfuAI | ACCTGC | 1 cut(s) | 1596 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 532 |
| BglII | AGATCT | 1 cut(s) | 547 |
| BisI | GCNGC | 4 cut(s) | 207, 394, 669, 1004 |
| BlsI | GCNGC | 4 cut(s) | 208, 395, 670, 1005 |
| BmcAI | AGTACT | 1 cut(s) | 227 |
| Bme1390I | CCNGG | 2 cut(s) | 572, 618 |
| Bme18I | GGWCC | 1 cut(s) | 1175 |
| BmgT120I | GGNCC | 2 cut(s) | 899, 1175 |
| BmiI | GGNNCC | 1 cut(s) | 869 |
| BmrFI | CCNGG | 2 cut(s) | 572, 618 |
| BmrI | ACTGGG | 3 cut(s) | 475, 1049, 1110 |
| BmsI | GCATC | 6 cut(s) | 784, 1207, 1405, 1427, 1466, 1534 |
| BmuI | ACTGGG | 3 cut(s) | 475, 1049, 1110 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1180 |
| BpiI | GAAGAC | 2 cut(s) | 511, 513 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 62, 94 |
| BpmI | CTGGAG | 1 cut(s) | 293 |
| Bpu14I | TTCGAA | 1 cut(s) | 1425 |
| BpuEI | CTTGAG | 1 cut(s) | 1566 |
| Bsa29I | ATCGAT | 2 cut(s) | 366, 595 |
| BsaAI | YACGTR | 1 cut(s) | 1535 |
| BsaJI | CCNNGG | 2 cut(s) | 774, 1468 |
| BsaWI | WCCGGW | 2 cut(s) | 353, 1357 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 956, 1458 |
| Bse1I | ACTGG | 7 cut(s) | 135, 335, 470, 753, 961, 1044, 1116 |
| Bse21I | CCTNAGG | 1 cut(s) | 1313 |
| BseAI | TCCGGA | 1 cut(s) | 353 |
| BseBI | CCWGG | 2 cut(s) | 572, 618 |
| BseCI | ATCGAT | 2 cut(s) | 366, 595 |
| BseDI | CCNNGG | 2 cut(s) | 774, 1468 |
| BseGI | GGATG | 4 cut(s) | 94, 1208, 1345, 1481 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 956, 1458 |
| BseNI | ACTGG | 7 cut(s) | 135, 335, 470, 753, 961, 1044, 1116 |
| BseXI | GCAGC | 2 cut(s) | 218, 680 |
| BsgI | GTGCAG | 1 cut(s) | 873 |
| BshFI | GGCC | 3 cut(s) | 526, 901, 960 |
| BshVI | ATCGAT | 2 cut(s) | 366, 595 |
| BsiHKAI | GWGCWC | 2 cut(s) | 390, 1345 |
| BsiSI | CCGG | 2 cut(s) | 354, 1358 |
| BslFI | GGGAC | 1 cut(s) | 482 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 956, 1458 |
| BsmFI | GGGAC | 1 cut(s) | 482 |
| BsnI | GGCC | 3 cut(s) | 526, 901, 960 |
| Bsp119I | TTCGAA | 1 cut(s) | 1425 |
| Bsp1286I | GDGCHC | 3 cut(s) | 390, 872, 1345 |
| Bsp13I | TCCGGA | 1 cut(s) | 353 |
| Bsp1407I | TGTACA | 1 cut(s) | 823 |
| Bsp143I | GATC | 5 cut(s) | 139, 405, 547, 1205, 1521 |
| Bsp19I | CCATGG | 1 cut(s) | 1468 |
| BspACI | CCGC | 4 cut(s) | 393, 633, 1004, 1287 |
| BspANI | GGCC | 3 cut(s) | 526, 901, 960 |
| BspDI | ATCGAT | 2 cut(s) | 366, 595 |
| BspEI | TCCGGA | 1 cut(s) | 353 |
| BspHI | TCATGA | 2 cut(s) | 100, 940 |
| BspLI | GGNNCC | 1 cut(s) | 869 |
| BspMI | ACCTGC | 1 cut(s) | 1596 |
| BspPI | GGATC | 2 cut(s) | 134, 1529 |
| BspT104I | TTCGAA | 1 cut(s) | 1425 |
| BsrGI | TGTACA | 1 cut(s) | 823 |
| BsrI | ACTGG | 7 cut(s) | 135, 335, 470, 753, 961, 1044, 1116 |
| BssECI | CCNNGG | 2 cut(s) | 774, 1468 |
| BssMI | GATC | 5 cut(s) | 139, 405, 547, 1205, 1521 |
| BssSI | CACGAG | 1 cut(s) | 397 |
| BssT1I | CCWWGG | 1 cut(s) | 1468 |
| Bst2BI | CACGAG | 1 cut(s) | 397 |
| Bst2UI | CCWGG | 2 cut(s) | 572, 618 |
| Bst4CI | ACNGT | 4 cut(s) | 540, 730, 1447, 1505 |
| Bst6I | CTCTTC | 1 cut(s) | 754 |
| BstAUI | TGTACA | 1 cut(s) | 823 |
| BstBAI | YACGTR | 1 cut(s) | 1535 |
| BstBI | TTCGAA | 1 cut(s) | 1425 |
| BstC8I | GCNNGC | 3 cut(s) | 415, 1569, 1573 |
| BstDEI | CTNAG | 2 cut(s) | 831, 1313 |
| BstDSI | CCRYGG | 2 cut(s) | 774, 1468 |
| BstF5I | GGATG | 4 cut(s) | 94, 1208, 1345, 1481 |
| BstH2I | RGCGCY | 1 cut(s) | 255 |
| BstHHI | GCGC | 1 cut(s) | 254 |
| BstKTI | GATC | 5 cut(s) | 142, 408, 550, 1208, 1524 |
| BstMBI | GATC | 5 cut(s) | 139, 405, 547, 1205, 1521 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 399, 532, 541, 1009 |
| BstNI | CCWGG | 2 cut(s) | 572, 618 |
| BstNSI | RCATGY | 1 cut(s) | 668 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1180 |
| BstSCI | CCNGG | 2 cut(s) | 570, 616 |
| BstSFI | CTRYAG | 3 cut(s) | 261, 285, 1446 |
| BstV1I | GCAGC | 2 cut(s) | 218, 680 |
| BstV2I | GAAGAC | 2 cut(s) | 511, 513 |
| BstX2I | RGATCY | 1 cut(s) | 547 |
| BstYI | RGATCY | 1 cut(s) | 547 |
| Bsu15I | ATCGAT | 2 cut(s) | 366, 595 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1313 |
| BsuRI | GGCC | 3 cut(s) | 526, 901, 960 |
| BsuTUI | ATCGAT | 2 cut(s) | 366, 595 |
| BtgI | CCRYGG | 2 cut(s) | 774, 1468 |
| BtsCI | GGATG | 4 cut(s) | 94, 1208, 1345, 1481 |
| BtsIMutI | CAGTG | 3 cut(s) | 142, 545, 789 |
| BveI | ACCTGC | 1 cut(s) | 1596 |
| Cac8I | GCNNGC | 3 cut(s) | 415, 1569, 1573 |
| CciI | TCATGA | 2 cut(s) | 100, 940 |
| CfoI | GCGC | 1 cut(s) | 254 |
| Cfr13I | GGNCC | 2 cut(s) | 899, 1175 |
| ClaI | ATCGAT | 2 cut(s) | 366, 595 |
| Csp6I | GTAC | 3 cut(s) | 226, 374, 824 |
| CviAII | CATG | 9 cut(s) | 101, 403, 472, 560, 665, 722, 941, 1469, 1564 |
| CviQI | GTAC | 3 cut(s) | 226, 374, 824 |
| DdeI | CTNAG | 2 cut(s) | 831, 1313 |
| DpnI | GATC | 5 cut(s) | 141, 407, 549, 1207, 1523 |
| DpnII | GATC | 5 cut(s) | 139, 405, 547, 1205, 1521 |
| DraI | TTTAAA | 1 cut(s) | 1329 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 515 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 515 |
| EaeI | YGGCCR | 1 cut(s) | 958 |
| Eam1104I | CTCTTC | 1 cut(s) | 754 |
| EarI | CTCTTC | 1 cut(s) | 754 |
| Ecl136II | GAGCTC | 1 cut(s) | 1343 |
| Eco130I | CCWWGG | 1 cut(s) | 1468 |
| Eco24I | GRGCYC | 2 cut(s) | 872, 1345 |
| Eco47I | GGWCC | 1 cut(s) | 1175 |
| Eco53kI | GAGCTC | 1 cut(s) | 1343 |
| Eco57I | CTGAAG | 2 cut(s) | 44, 505 |
| Eco81I | CCTNAGG | 1 cut(s) | 1313 |
| EcoICRI | GAGCTC | 1 cut(s) | 1343 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1175 |
| EcoRII | CCWGG | 2 cut(s) | 570, 616 |
| EcoT14I | CCWWGG | 1 cut(s) | 1468 |
| EcoT22I | ATGCAT | 1 cut(s) | 1420 |
| EcoT38I | GRGCYC | 2 cut(s) | 872, 1345 |
| ErhI | CCWWGG | 1 cut(s) | 1468 |
| FaeI | CATG | 9 cut(s) | 104, 406, 475, 563, 668, 725, 944, 1472, 1567 |
| FaqI | GGGAC | 1 cut(s) | 482 |
| FatI | CATG | 9 cut(s) | 100, 402, 471, 559, 664, 721, 940, 1468, 1563 |
| FauNDI | CATATG | 3 cut(s) | 160, 1027, 1186 |
| FbaI | TGATCA | 1 cut(s) | 405 |
| Fnu4HI | GCNGC | 4 cut(s) | 207, 394, 669, 1004 |
| FokI | GGATG | 4 cut(s) | 81, 1195, 1332, 1488 |
| FriOI | GRGCYC | 2 cut(s) | 872, 1345 |
| Fsp4HI | GCNGC | 4 cut(s) | 207, 394, 669, 1004 |
| GlaI | GCGC | 1 cut(s) | 253 |
| GluI | GCNGC | 4 cut(s) | 207, 394, 669, 1004 |
| GsuI | CTGGAG | 1 cut(s) | 293 |
| HaeII | RGCGCY | 1 cut(s) | 255 |
| HaeIII | GGCC | 3 cut(s) | 526, 901, 960 |
| HapII | CCGG | 2 cut(s) | 354, 1358 |
| HhaI | GCGC | 1 cut(s) | 254 |
| Hin1II | CATG | 9 cut(s) | 104, 406, 475, 563, 668, 725, 944, 1472, 1567 |
| Hin6I | GCGC | 1 cut(s) | 252 |
| HinP1I | GCGC | 1 cut(s) | 252 |
| HincII | GTYRAC | 3 cut(s) | 282, 994, 1162 |
| HindII | GTYRAC | 3 cut(s) | 282, 994, 1162 |
| HinfI | GANTC | 6 cut(s) | 597, 607, 627, 780, 905, 1526 |
| HpaI | GTTAAC | 2 cut(s) | 994, 1162 |
| HpaII | CCGG | 2 cut(s) | 354, 1358 |
| HphI | GGTGA | 2 cut(s) | 622, 1220 |
| Hpy166II | GTNNAC | 7 cut(s) | 76, 127, 282, 826, 994, 1162, 1228 |
| Hpy188I | TCNGA | 6 cut(s) | 70, 220, 1263, 1385, 1411, 1440 |
| Hpy188III | TCNNGA | 9 cut(s) | 101, 143, 242, 354, 456, 748, 941, 1075, 1173 |
| Hpy8I | GTNNAC | 7 cut(s) | 76, 127, 282, 826, 994, 1162, 1228 |
| HpyAV | CCTTC | 5 cut(s) | 62, 146, 375, 1483, 1569 |
| HpyCH4III | ACNGT | 4 cut(s) | 540, 730, 1447, 1505 |
| HpyCH4IV | ACGT | 2 cut(s) | 1199, 1534 |
| HpyCH4V | TGCA | 5 cut(s) | 668, 854, 925, 1418, 1630 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 399, 532, 541, 1009 |
| HpyF3I | CTNAG | 2 cut(s) | 831, 1313 |
| HpySE526I | ACGT | 2 cut(s) | 1199, 1534 |
| Hsp92II | CATG | 9 cut(s) | 104, 406, 475, 563, 668, 725, 944, 1472, 1567 |
| HspAI | GCGC | 1 cut(s) | 252 |
| Kpn2I | TCCGGA | 1 cut(s) | 353 |
| Ksp22I | TGATCA | 1 cut(s) | 405 |
| KspAI | GTTAAC | 2 cut(s) | 994, 1162 |
| Kzo9I | GATC | 5 cut(s) | 139, 405, 547, 1205, 1521 |
| LmnI | GCTCC | 1 cut(s) | 867 |
| Lsp1109I | GCAGC | 2 cut(s) | 218, 680 |
| LweI | GCATC | 6 cut(s) | 784, 1207, 1405, 1427, 1466, 1534 |
| MaeII | ACGT | 2 cut(s) | 1199, 1534 |
| MaeIII | GTNAC | 3 cut(s) | 1030, 1195, 1200 |
| MalI | GATC | 5 cut(s) | 141, 407, 549, 1207, 1523 |
| MboI | GATC | 5 cut(s) | 139, 405, 547, 1205, 1521 |
| MboII | GAAGA | 7 cut(s) | 50, 329, 511, 518, 736, 771, 1372 |
| MfeI | CAATTG | 1 cut(s) | 861 |
| MflI | RGATCY | 1 cut(s) | 547 |
| MhlI | GDGCHC | 3 cut(s) | 390, 872, 1345 |
| MlsI | TGGCCA | 1 cut(s) | 960 |
| MluNI | TGGCCA | 1 cut(s) | 960 |
| MlyI | GAGTC | 4 cut(s) | 621, 789, 914, 1520 |
| MmeI | TCCRAC | 4 cut(s) | 111, 694, 1286, 1418 |
| Mox20I | TGGCCA | 1 cut(s) | 960 |
| Mph1103I | ATGCAT | 1 cut(s) | 1420 |
| MroI | TCCGGA | 1 cut(s) | 353 |
| MroXI | GAANNNNTTC | 1 cut(s) | 763 |
| MscI | TGGCCA | 1 cut(s) | 960 |
| MseI | TTAA | 7 cut(s) | 483, 684, 936, 993, 1161, 1328, 1620 |
| MslI | CAYNNNNRTG | 6 cut(s) | 99, 193, 401, 496, 1233, 1467 |
| Msp20I | TGGCCA | 1 cut(s) | 960 |
| MspA1I | CMGCKG | 1 cut(s) | 671 |
| MspI | CCGG | 2 cut(s) | 354, 1358 |
| MspR9I | CCNGG | 2 cut(s) | 572, 618 |
| MunI | CAATTG | 1 cut(s) | 861 |
| MvaI | CCWGG | 2 cut(s) | 572, 618 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 399, 532, 541, 1009 |
| NcoI | CCATGG | 1 cut(s) | 1468 |
| NdeI | CATATG | 3 cut(s) | 160, 1027, 1186 |
| NdeII | GATC | 5 cut(s) | 139, 405, 547, 1205, 1521 |
| NlaIII | CATG | 9 cut(s) | 104, 406, 475, 563, 668, 725, 944, 1472, 1567 |
| NlaIV | GGNNCC | 1 cut(s) | 869 |
| NmeAIII | GCCGAG | 1 cut(s) | 369 |
| NmuCI | GTSAC | 1 cut(s) | 1195 |
| NsiI | ATGCAT | 1 cut(s) | 1420 |
| NspI | RCATGY | 1 cut(s) | 668 |
| NspV | TTCGAA | 1 cut(s) | 1425 |
| PagI | TCATGA | 2 cut(s) | 100, 940 |
| PdmI | GAANNNNTTC | 1 cut(s) | 763 |
| PfeI | GAWTC | 2 cut(s) | 597, 607 |
| PkrI | GCNGC | 4 cut(s) | 208, 395, 670, 1005 |
| PleI | GAGTC | 4 cut(s) | 621, 788, 913, 1520 |
| PpsI | GAGTC | 4 cut(s) | 621, 788, 913, 1520 |
| Ppu21I | YACGTR | 1 cut(s) | 1535 |
| PpuMI | RGGWCCY | 1 cut(s) | 1175 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1180 |
| Psp124BI | GAGCTC | 1 cut(s) | 1345 |
| Psp5II | RGGWCCY | 1 cut(s) | 1175 |
| Psp6I | CCWGG | 2 cut(s) | 570, 616 |
| PspGI | CCWGG | 2 cut(s) | 570, 616 |
| PspN4I | GGNNCC | 1 cut(s) | 869 |
| PspPI | GGNCC | 2 cut(s) | 899, 1175 |
| PspPPI | RGGWCCY | 1 cut(s) | 1175 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 748, 780 |
| PsuI | RGATCY | 1 cut(s) | 547 |
| PvuII | CAGCTG | 1 cut(s) | 671 |
| RsaI | GTAC | 3 cut(s) | 227, 375, 825 |
| RsaNI | GTAC | 3 cut(s) | 226, 374, 824 |
| RseI | CAYNNNNRTG | 6 cut(s) | 99, 193, 401, 496, 1233, 1467 |
| SacI | GAGCTC | 1 cut(s) | 1345 |
| SaqAI | TTAA | 7 cut(s) | 483, 684, 936, 993, 1161, 1328, 1620 |
| SatI | GCNGC | 4 cut(s) | 207, 394, 669, 1004 |
| Sau3AI | GATC | 5 cut(s) | 139, 405, 547, 1205, 1521 |
| Sau96I | GGNCC | 2 cut(s) | 899, 1175 |
| ScaI | AGTACT | 1 cut(s) | 227 |
| SchI | GAGTC | 4 cut(s) | 621, 789, 914, 1520 |
| ScrFI | CCNGG | 2 cut(s) | 572, 618 |
| SduI | GDGCHC | 3 cut(s) | 390, 872, 1345 |
| SfaNI | GCATC | 6 cut(s) | 784, 1207, 1405, 1427, 1466, 1534 |
| SfcI | CTRYAG | 3 cut(s) | 261, 285, 1446 |
| SfuI | TTCGAA | 1 cut(s) | 1425 |
| SinI | GGWCC | 1 cut(s) | 1175 |
| SmiMI | CAYNNNNRTG | 6 cut(s) | 99, 193, 401, 496, 1233, 1467 |
| SmlI | CTYRAG | 1 cut(s) | 1581 |
| SmoI | CTYRAG | 1 cut(s) | 1581 |
| SsiI | CCGC | 4 cut(s) | 393, 633, 1004, 1287 |
| SspI | AATATT | 1 cut(s) | 1300 |
| SstI | GAGCTC | 1 cut(s) | 1345 |
| StyD4I | CCNGG | 2 cut(s) | 570, 616 |
| StyI | CCWWGG | 1 cut(s) | 1468 |
| TaaI | ACNGT | 4 cut(s) | 540, 730, 1447, 1505 |
| TaiI | ACGT | 2 cut(s) | 1202, 1537 |
| TaqI | TCGA | 8 cut(s) | 366, 595, 645, 1076, 1082, 1142, 1425, 1524 |
| TatI | WGTACW | 2 cut(s) | 225, 823 |
| TauI | GCSGC | 2 cut(s) | 396, 1006 |
| TfiI | GAWTC | 2 cut(s) | 597, 607 |
| Tru1I | TTAA | 7 cut(s) | 483, 684, 936, 993, 1161, 1328, 1620 |
| Tru9I | TTAA | 7 cut(s) | 483, 684, 936, 993, 1161, 1328, 1620 |
| TscAI | CASTG | 3 cut(s) | 142, 545, 796 |
| TseFI | GTSAC | 1 cut(s) | 1195 |
| TseI | GCWGC | 2 cut(s) | 206, 668 |
| Tsp45I | GTSAC | 1 cut(s) | 1195 |
| TspDTI | ATGAA | 6 cut(s) | 44, 189, 429, 690, 710, 1307 |
| TspGWI | ACGGA | 2 cut(s) | 339, 1049 |
| TspRI | CASTG | 3 cut(s) | 142, 545, 796 |
| VpaK11BI | GGWCC | 1 cut(s) | 1175 |
| XapI | RAATTY | 3 cut(s) | 119, 1167, 1430 |
| XceI | RCATGY | 1 cut(s) | 668 |
| XmnI | GAANNNNTTC | 1 cut(s) | 763 |
| ZrmI | AGTACT | 1 cut(s) | 227 |
| Zsp2I | ATGCAT | 1 cut(s) | 1420 |