(R)-mandelonitrile lyase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 1141 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 356 |
| AciI | CCGC | 2 cut(s) | 925, 1113 |
| AclWI | GGATC | 2 cut(s) | 378, 496 |
| AcsI | RAATTY | 5 cut(s) | 101, 197, 286, 447, 1070 |
| AcuI | CTGAAG | 1 cut(s) | 902 |
| AfaI | GTAC | 4 cut(s) | 298, 555, 877, 1250 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 356, 686 |
| AgsI | TTSAA | 8 cut(s) | 98, 106, 263, 407, 655, 890, 931, 1198 |
| AluBI | AGCT | 4 cut(s) | 188, 401, 886, 1146 |
| AluI | AGCT | 4 cut(s) | 188, 401, 886, 1146 |
| Alw26I | GTCTC | 4 cut(s) | 280, 385, 543, 791 |
| AlwI | GGATC | 2 cut(s) | 378, 496 |
| Ama87I | CYCGRG | 1 cut(s) | 504 |
| AoxI | GGCC | 3 cut(s) | 628, 750, 1224 |
| ApeKI | GCWGC | 2 cut(s) | 1143, 1267 |
| ApoI | RAATTY | 5 cut(s) | 101, 197, 286, 447, 1070 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 521, 553 |
| AspS9I | GGNCC | 5 cut(s) | 347, 629, 914, 949, 1025 |
| AsuC2I | CCSGG | 1 cut(s) | 1209 |
| AsuHPI | GGTGA | 3 cut(s) | 523, 572, 662 |
| AsuII | TTCGAA | 1 cut(s) | 527 |
| AvaI | CYCGRG | 1 cut(s) | 504 |
| AvaII | GGWCC | 4 cut(s) | 347, 914, 949, 1025 |
| BbvI | GCAGC | 2 cut(s) | 1155, 1254 |
| BccI | CCATC | 2 cut(s) | 71, 110 |
| BceAI | ACGGC | 1 cut(s) | 1126 |
| BclI | TGATCA | 1 cut(s) | 162 |
| BcnI | CCSGG | 1 cut(s) | 1209 |
| BcoDI | GTCTC | 4 cut(s) | 280, 385, 543, 791 |
| BfaI | CTAG | 1 cut(s) | 807 |
| BfmI | CTRYAG | 2 cut(s) | 746, 832 |
| BfuAI | ACCTGC | 1 cut(s) | 1141 |
| BisI | GCNGC | 2 cut(s) | 1144, 1268 |
| BlsI | GCNGC | 2 cut(s) | 1145, 1269 |
| Bme1390I | CCNGG | 1 cut(s) | 1209 |
| Bme18I | GGWCC | 4 cut(s) | 347, 914, 949, 1025 |
| BmeT110I | CYCGRG | 1 cut(s) | 504 |
| BmgT120I | GGNCC | 5 cut(s) | 347, 629, 914, 949, 1025 |
| BmrFI | CCNGG | 1 cut(s) | 1209 |
| BmrI | ACTGGG | 2 cut(s) | 265, 656 |
| BmsI | GCATC | 6 cut(s) | 1045, 1067, 1106, 1156, 1196, 1254 |
| BmuI | ACTGGG | 2 cut(s) | 265, 656 |
| BoxI | GACNNNNGTC | 1 cut(s) | 865 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 788, 820 |
| Bpu14I | TTCGAA | 1 cut(s) | 527 |
| BpuEI | CTTGAG | 1 cut(s) | 139 |
| BpuMI | CCSGG | 1 cut(s) | 1209 |
| Bsa29I | ATCGAT | 2 cut(s) | 25, 1164 |
| BsaAI | YACGTR | 1 cut(s) | 557 |
| BsaI | GGTCTC | 2 cut(s) | 543, 791 |
| BsaJI | CCNNGG | 3 cut(s) | 945, 1108, 1208 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 356, 686 |
| Bse1I | ACTGG | 4 cut(s) | 92, 260, 662, 691 |
| BseCI | ATCGAT | 2 cut(s) | 25, 1164 |
| BseDI | CCNNGG | 3 cut(s) | 945, 1108, 1208 |
| BseGI | GGATG | 3 cut(s) | 940, 1121, 1187 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 356, 686 |
| BseMII | CTCAG | 2 cut(s) | 569, 617 |
| BseNI | ACTGG | 4 cut(s) | 92, 260, 662, 691 |
| BseXI | GCAGC | 2 cut(s) | 1155, 1254 |
| BsgI | GTGCAG | 1 cut(s) | 603 |
| BshFI | GGCC | 3 cut(s) | 630, 752, 1226 |
| BshVI | ATCGAT | 2 cut(s) | 25, 1164 |
| BsiHKCI | CYCGRG | 1 cut(s) | 504 |
| BsiSI | CCGG | 1 cut(s) | 1208 |
| BslFI | GGGAC | 3 cut(s) | 239, 718, 1225 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 356, 686 |
| BsmAI | GTCTC | 4 cut(s) | 280, 385, 543, 791 |
| BsmBI | CGTCTC | 1 cut(s) | 280 |
| BsmFI | GGGAC | 3 cut(s) | 239, 718, 1225 |
| BsnI | GGCC | 3 cut(s) | 630, 752, 1226 |
| Bso31I | GGTCTC | 2 cut(s) | 543, 791 |
| BsoBI | CYCGRG | 1 cut(s) | 504 |
| Bsp119I | TTCGAA | 1 cut(s) | 527 |
| Bsp1407I | TGTACA | 1 cut(s) | 296 |
| Bsp143I | GATC | 4 cut(s) | 162, 370, 468, 488 |
| BspACI | CCGC | 2 cut(s) | 925, 1113 |
| BspANI | GGCC | 3 cut(s) | 630, 752, 1226 |
| BspCNI | CTCAG | 2 cut(s) | 570, 616 |
| BspDI | ATCGAT | 2 cut(s) | 25, 1164 |
| BspMI | ACCTGC | 1 cut(s) | 1141 |
| BspPI | GGATC | 2 cut(s) | 378, 496 |
| BspT104I | TTCGAA | 1 cut(s) | 527 |
| BspTNI | GGTCTC | 2 cut(s) | 543, 791 |
| BsrGI | TGTACA | 1 cut(s) | 296 |
| BsrI | ACTGG | 4 cut(s) | 92, 260, 662, 691 |
| BssECI | CCNNGG | 3 cut(s) | 945, 1108, 1208 |
| BssMI | GATC | 4 cut(s) | 162, 370, 468, 488 |
| BssT1I | CCWWGG | 1 cut(s) | 945 |
| Bst4CI | ACNGT | 3 cut(s) | 460, 760, 1087 |
| Bst6I | CTCTTC | 1 cut(s) | 484 |
| BstAUI | TGTACA | 1 cut(s) | 296 |
| BstBAI | YACGTR | 1 cut(s) | 557 |
| BstBI | TTCGAA | 1 cut(s) | 527 |
| BstC8I | GCNNGC | 3 cut(s) | 812, 958, 1148 |
| BstDEI | CTNAG | 3 cut(s) | 578, 603, 617 |
| BstDSI | CCRYGG | 1 cut(s) | 1108 |
| BstF5I | GGATG | 3 cut(s) | 940, 1121, 1187 |
| BstKTI | GATC | 4 cut(s) | 165, 373, 471, 491 |
| BstMAI | GTCTC | 4 cut(s) | 280, 385, 543, 791 |
| BstMBI | GATC | 4 cut(s) | 162, 370, 468, 488 |
| BstNSI | RCATGY | 1 cut(s) | 814 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 865 |
| BstSCI | CCNGG | 1 cut(s) | 1207 |
| BstSFI | CTRYAG | 2 cut(s) | 746, 832 |
| BstV1I | GCAGC | 2 cut(s) | 1155, 1254 |
| Bsu15I | ATCGAT | 2 cut(s) | 25, 1164 |
| BsuRI | GGCC | 3 cut(s) | 630, 752, 1226 |
| BsuTUI | ATCGAT | 2 cut(s) | 25, 1164 |
| BtgI | CCRYGG | 1 cut(s) | 1108 |
| BtsCI | GGATG | 3 cut(s) | 940, 1121, 1187 |
| BtsIMutI | CAGTG | 4 cut(s) | 253, 465, 684, 1007 |
| BveI | ACCTGC | 1 cut(s) | 1141 |
| Cac8I | GCNNGC | 3 cut(s) | 812, 958, 1148 |
| Cfr13I | GGNCC | 5 cut(s) | 347, 629, 914, 949, 1025 |
| ClaI | ATCGAT | 2 cut(s) | 25, 1164 |
| CseI | GACGC | 1 cut(s) | 1193 |
| Csp6I | GTAC | 4 cut(s) | 297, 554, 876, 1249 |
| CviAII | CATG | 8 cut(s) | 64, 160, 229, 294, 395, 452, 811, 973 |
| CviJI | RGCY | 9 cut(s) | 153, 188, 401, 503, 630, 752, 886, 1146, 1226 |
| CviKI_1 | RGCY | 9 cut(s) | 153, 188, 401, 503, 630, 752, 886, 1146, 1226 |
| CviQI | GTAC | 4 cut(s) | 297, 554, 876, 1249 |
| DdeI | CTNAG | 3 cut(s) | 578, 603, 617 |
| DpnI | GATC | 4 cut(s) | 164, 372, 470, 490 |
| DpnII | GATC | 4 cut(s) | 162, 370, 468, 488 |
| Eam1104I | CTCTTC | 1 cut(s) | 484 |
| EarI | CTCTTC | 1 cut(s) | 484 |
| EciI | GGCGGA | 1 cut(s) | 1128 |
| Eco130I | CCWWGG | 1 cut(s) | 945 |
| Eco147I | AGGCCT | 1 cut(s) | 752 |
| Eco31I | GGTCTC | 2 cut(s) | 543, 791 |
| Eco47I | GGWCC | 4 cut(s) | 347, 914, 949, 1025 |
| Eco57I | CTGAAG | 1 cut(s) | 902 |
| Eco88I | CYCGRG | 1 cut(s) | 504 |
| EcoRI | GAATTC | 3 cut(s) | 197, 447, 1070 |
| EcoT14I | CCWWGG | 1 cut(s) | 945 |
| EcoT22I | ATGCAT | 1 cut(s) | 1060 |
| ErhI | CCWWGG | 1 cut(s) | 945 |
| Esp3I | CGTCTC | 1 cut(s) | 280 |
| FaeI | CATG | 8 cut(s) | 67, 163, 232, 297, 398, 455, 814, 976 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 458, 490 |
| FaqI | GGGAC | 3 cut(s) | 239, 718, 1225 |
| FatI | CATG | 8 cut(s) | 63, 159, 228, 293, 394, 451, 810, 972 |
| FbaI | TGATCA | 1 cut(s) | 162 |
| Fnu4HI | GCNGC | 2 cut(s) | 1144, 1268 |
| FokI | GGATG | 3 cut(s) | 927, 1128, 1174 |
| Fsp4HI | GCNGC | 2 cut(s) | 1144, 1268 |
| FspBI | CTAG | 1 cut(s) | 807 |
| GluI | GCNGC | 2 cut(s) | 1144, 1268 |
| HaeIII | GGCC | 3 cut(s) | 630, 752, 1226 |
| HapII | CCGG | 1 cut(s) | 1208 |
| HgaI | GACGC | 1 cut(s) | 1193 |
| Hin1II | CATG | 8 cut(s) | 67, 163, 232, 297, 398, 455, 814, 976 |
| HincII | GTYRAC | 1 cut(s) | 1183 |
| HindII | GTYRAC | 1 cut(s) | 1183 |
| HindIII | AAGCTT | 1 cut(s) | 884 |
| HinfI | GANTC | 3 cut(s) | 598, 837, 1161 |
| HpaII | CCGG | 1 cut(s) | 1208 |
| HphI | GGTGA | 3 cut(s) | 523, 572, 662 |
| Hpy166II | GTNNAC | 2 cut(s) | 670, 1183 |
| Hpy188I | TCNGA | 6 cut(s) | 112, 134, 579, 882, 901, 1127 |
| Hpy188III | TCNNGA | 2 cut(s) | 213, 1079 |
| Hpy8I | GTNNAC | 2 cut(s) | 670, 1183 |
| Hpy99I | CGWCG | 1 cut(s) | 1127 |
| HpyAV | CCTTC | 2 cut(s) | 557, 811 |
| HpyCH4III | ACNGT | 3 cut(s) | 460, 760, 1087 |
| HpyCH4IV | ACGT | 2 cut(s) | 426, 556 |
| HpyCH4V | TGCA | 6 cut(s) | 67, 584, 938, 1058, 1150, 1270 |
| HpyF3I | CTNAG | 3 cut(s) | 578, 603, 617 |
| HpySE526I | ACGT | 2 cut(s) | 426, 556 |
| Hsp92II | CATG | 8 cut(s) | 67, 163, 232, 297, 398, 455, 814, 976 |
| Ksp22I | TGATCA | 1 cut(s) | 162 |
| Kzo9I | GATC | 4 cut(s) | 162, 370, 468, 488 |
| LmnI | GCTCC | 1 cut(s) | 398 |
| Lsp1109I | GCAGC | 2 cut(s) | 1155, 1254 |
| LweI | GCATC | 6 cut(s) | 1045, 1067, 1106, 1156, 1196, 1254 |
| MaeI | CTAG | 1 cut(s) | 807 |
| MaeII | ACGT | 2 cut(s) | 426, 556 |
| MaeIII | GTNAC | 2 cut(s) | 55, 1005 |
| MalI | GATC | 4 cut(s) | 164, 372, 470, 490 |
| MboI | GATC | 4 cut(s) | 162, 370, 468, 488 |
| MboII | GAAGA | 7 cut(s) | 83, 125, 466, 471, 478, 988, 991 |
| MfeI | CAATTG | 1 cut(s) | 591 |
| MlyI | GAGTC | 2 cut(s) | 607, 831 |
| MmeI | TCCRAC | 3 cut(s) | 312, 1052, 1105 |
| Mph1103I | ATGCAT | 1 cut(s) | 1060 |
| MseI | TTAA | 8 cut(s) | 41, 207, 219, 362, 378, 414, 995, 1260 |
| MslI | CAYNNNNRTG | 3 cut(s) | 789, 1232, 1262 |
| MspI | CCGG | 1 cut(s) | 1208 |
| MspR9I | CCNGG | 1 cut(s) | 1209 |
| MunI | CAATTG | 1 cut(s) | 591 |
| NciI | CCSGG | 1 cut(s) | 1209 |
| NdeII | GATC | 4 cut(s) | 162, 370, 468, 488 |
| NlaIII | CATG | 8 cut(s) | 67, 163, 232, 297, 398, 455, 814, 976 |
| NmuCI | GTSAC | 2 cut(s) | 55, 1005 |
| NsiI | ATGCAT | 1 cut(s) | 1060 |
| NspI | RCATGY | 1 cut(s) | 814 |
| NspV | TTCGAA | 1 cut(s) | 527 |
| PaeI | GCATGC | 1 cut(s) | 814 |
| PaeR7I | CTCGAG | 1 cut(s) | 504 |
| PceI | AGGCCT | 1 cut(s) | 752 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 777 |
| PfeI | GAWTC | 1 cut(s) | 1161 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 356 |
| PkrI | GCNGC | 2 cut(s) | 1145, 1269 |
| PleI | GAGTC | 2 cut(s) | 606, 831 |
| PpsI | GAGTC | 2 cut(s) | 606, 831 |
| Ppu21I | YACGTR | 1 cut(s) | 557 |
| PshAI | GACNNNNGTC | 1 cut(s) | 865 |
| PspPI | GGNCC | 5 cut(s) | 347, 629, 914, 949, 1025 |
| PspXI | VCTCGAGB | 1 cut(s) | 504 |
| RsaI | GTAC | 4 cut(s) | 298, 555, 877, 1250 |
| RsaNI | GTAC | 4 cut(s) | 297, 554, 876, 1249 |
| RseI | CAYNNNNRTG | 3 cut(s) | 789, 1232, 1262 |
| SaqAI | TTAA | 8 cut(s) | 41, 207, 219, 362, 378, 414, 995, 1260 |
| SatI | GCNGC | 2 cut(s) | 1144, 1268 |
| Sau3AI | GATC | 4 cut(s) | 162, 370, 468, 488 |
| Sau96I | GGNCC | 5 cut(s) | 347, 629, 914, 949, 1025 |
| SchI | GAGTC | 2 cut(s) | 607, 831 |
| ScrFI | CCNGG | 1 cut(s) | 1209 |
| SfaNI | GCATC | 6 cut(s) | 1045, 1067, 1106, 1156, 1196, 1254 |
| SfcI | CTRYAG | 2 cut(s) | 746, 832 |
| Sfr274I | CTCGAG | 1 cut(s) | 504 |
| SfuI | TTCGAA | 1 cut(s) | 527 |
| SinI | GGWCC | 4 cut(s) | 347, 914, 949, 1025 |
| SlaI | CTCGAG | 1 cut(s) | 504 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 789, 1232, 1262 |
| SmlI | CTYRAG | 2 cut(s) | 154, 504 |
| SmoI | CTYRAG | 2 cut(s) | 154, 504 |
| SphI | GCATGC | 1 cut(s) | 814 |
| SseBI | AGGCCT | 1 cut(s) | 752 |
| SsiI | CCGC | 2 cut(s) | 925, 1113 |
| SspMI | CTAG | 1 cut(s) | 807 |
| StuI | AGGCCT | 1 cut(s) | 752 |
| StyD4I | CCNGG | 1 cut(s) | 1207 |
| StyI | CCWWGG | 1 cut(s) | 945 |
| TaaI | ACNGT | 3 cut(s) | 460, 760, 1087 |
| TaiI | ACGT | 2 cut(s) | 429, 559 |
| TaqI | TCGA | 5 cut(s) | 25, 505, 527, 1137, 1164 |
| TatI | WGTACW | 1 cut(s) | 296 |
| TfiI | GAWTC | 1 cut(s) | 1161 |
| Tru1I | TTAA | 8 cut(s) | 41, 207, 219, 362, 378, 414, 995, 1260 |
| Tru9I | TTAA | 8 cut(s) | 41, 207, 219, 362, 378, 414, 995, 1260 |
| TscAI | CASTG | 4 cut(s) | 260, 465, 691, 1014 |
| TseFI | GTSAC | 2 cut(s) | 55, 1005 |
| TseI | GCWGC | 2 cut(s) | 1143, 1267 |
| Tsp45I | GTSAC | 2 cut(s) | 55, 1005 |
| TspDTI | ATGAA | 4 cut(s) | 440, 709, 835, 989 |
| TspGWI | ACGGA | 1 cut(s) | 1163 |
| TspRI | CASTG | 4 cut(s) | 260, 465, 691, 1014 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 356 |
| VpaK11BI | GGWCC | 4 cut(s) | 347, 914, 949, 1025 |
| XapI | RAATTY | 5 cut(s) | 101, 197, 286, 447, 1070 |
| XceI | RCATGY | 1 cut(s) | 814 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 683 |
| XhoI | CTCGAG | 1 cut(s) | 504 |
| XspI | CTAG | 1 cut(s) | 807 |
| Zsp2I | ATGCAT | 1 cut(s) | 1060 |