MD03G1090900.v1.1

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000533
Physical Location & Seq
Forward (+)
7527522 .. 7529143
1622 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1090900.v1.1.491

Sequence Viewer

Length: 1326 bp
ATGCAAGAAGATGACGGTAAGACGCCGGCCCAGAGGTTCACATCAGAAGATGGTGTAGCTAATCTAAGAGGACGGATCCTAGGCGGGTCAAGTATGATCAATATTGGTATATACTCAAGAGCCGACGGTGAATTTTACCAGAAATCAGGAATTAAATTGGACATGAACTTAGTCAATAATTCATATGAATGGGTTGAAAACACTGTAGCATTCCGTCCGAATGTAACACACTGGCAGTCTGTTGTGAAAGATGCAATGTTAGAAGCTGGTGTTCGTCCAGACAATGGACTGACTTTGGATCACATTCTGGGAAGTAAAGTCACGGCTACGCTCTTTGACGATCGTGGAAAAAGACATGGAGCTGTGGAACTACTAAATAAAGGACATCCAAAAAACCTGAGAGTTGCAATCCATGCCACAGTAGAGAGGATCATTTTCTCTTCCAAAGCATCAGGTTTGTCAGCTAAAGGAATCATATACAGTGATTCTAACGGGAGGTCTCATCGGGCATTGATACGTGGTAAGGGTGAGGTTATATTGAGTGCAGGTGCAATTGGAAGTCCTCAACTTCTACTACTTAGTGGTGTTGGCCCAAAATCTTACCTTTCATCTCGTAAAATCCCAGTTGTTCACCCACAACCTTACGTTGGGCAGTTTATGCGTGATAATCCTCGTAATTACATTACCATTTTGCCCCCGTTTCAAGTAGAAGCTTCTACTGCACAGGTCGTTGGTATCACAAGTGATTATTACATAGAGACTTTCTCCGGCTTGCCATTTTCTAGACAGGCCTTTAGTCTTTTCCCTAGTCCAACTATTCCCATGACCATAAATTCAAGTTTCGGGCACATTGTTGTCAAATTTCCAGGACCCTTGTCCTATGGTTCTCTTGATCTGCAATCATCCTATGATGTCAAAGTTGCTCCAAATGTCAAATTCAACTACTTTGCACAGGAGGCAGACCTTTCTCGTTGTGTTAGTGCCGTGAGGAAAATGGGTGATTTATTAAAAACAAACTCACTGAAACCGTATAAGGCTCAAGATTTGCCAGGTCTAGAAGGTTTCAACTTTTTTGGATTGCCTTTACCAGTGAATCAGTCAGACGATGCATCATTTGAAACCTTTTGTCGAGATACAGTAGCCACATTTTGGCACTACCATGGTGGATGCCTGGTCGGACAGGTAGTTGATGAAGATTTGCGGGTCATGGGGATCAAGGCATTACGTGTTGTTGATGGATCTGTATTCAATTTATCACCAGGGACAAATCCTCAAGCCACTATTATGATGTTGGGCAGGTATGCATGTTACATGTACTGCATTTAA

Protein Analysis

442

Amino Acids

48.36

Weight (kDa)

8.78

Isoelectric Point (pI)

39.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_N PF00732 22 - 218 2.6e-18 GMC oxidoreductase
GMC_oxred_C PF05199 291 - 432 1.5e-26 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 536
Acc36I ACCTGC 2 cut(s) 536, 1287
AccB7I CCANNNNNTGG 2 cut(s) 284, 1149
AciI CCGC 2 cut(s) 84, 1201
AclWI GGATC 6 cut(s) 70, 83, 306, 437, 1220, 1246
AcsI RAATTY 4 cut(s) 131, 832, 860, 935
AcyI GRCGYC 1 cut(s) 23
AfaI GTAC 1 cut(s) 1316
AfiI CCNNNNNNNGG 3 cut(s) 284, 647, 1149
AflIII ACRYGT 2 cut(s) 1225, 1311
AgsI TTSAA 7 cut(s) 197, 704, 837, 940, 1066, 1118, 1249
AjnI CCWGG 4 cut(s) 865, 1048, 1170, 1258
AluBI AGCT 5 cut(s) 59, 266, 362, 464, 713
AluI AGCT 5 cut(s) 59, 266, 362, 464, 713
Alw26I GTCTC 2 cut(s) 504, 752
AlwI GGATC 6 cut(s) 70, 83, 306, 437, 1220, 1246
AoxI GGCC 3 cut(s) 27, 589, 789
ApoI RAATTY 4 cut(s) 131, 832, 860, 935
AspA2I CCTAGG 1 cut(s) 79
AspS9I GGNCC 3 cut(s) 28, 590, 869
AsuHPI GGTGA 5 cut(s) 140, 539, 623, 1010, 1248
AvaII GGWCC 1 cut(s) 869
AvrII CCTAGG 1 cut(s) 79
BaeGI GKGCMC 1 cut(s) 849
BamHI GGATCC 1 cut(s) 75
BccI CCATC 2 cut(s) 44, 1229
BceAI ACGGC 2 cut(s) 339, 968
BciT130I CCWGG 4 cut(s) 867, 1050, 1172, 1260
BclI TGATCA 1 cut(s) 96
BcoDI GTCTC 2 cut(s) 504, 752
BfaI CTAG 4 cut(s) 80, 783, 807, 1055
BfmI CTRYAG 1 cut(s) 204
BfuAI ACCTGC 2 cut(s) 536, 1287
BlnI CCTAGG 1 cut(s) 79
Bme1390I CCNGG 4 cut(s) 867, 1050, 1172, 1260
Bme18I GGWCC 1 cut(s) 869
BmgT120I GGNCC 3 cut(s) 28, 590, 869
BmiI GGNNCC 2 cut(s) 77, 871
BmrFI CCNGG 4 cut(s) 867, 1050, 1172, 1260
BmrI ACTGGG 1 cut(s) 617
BmsI GCATC 5 cut(s) 241, 458, 1096, 1118, 1157
BmuI ACTGGG 1 cut(s) 617
BoxI GACNNNNGTC 1 cut(s) 874
BplI GAGNNNNNCTC 2 cut(s) 749, 781
BpuEI CTTGAG 3 cut(s) 100, 1023, 1257
BsaAI YACGTR 2 cut(s) 518, 1226
BsaHI GRCGYC 1 cut(s) 23
BsaI GGTCTC 1 cut(s) 504
BsaJI CCNNGG 3 cut(s) 79, 1159, 1259
Bsc4I CCNNNNNNNGG 3 cut(s) 284, 647, 1149
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 3 cut(s) 236, 623, 1088
Bse3DI GCAATG 1 cut(s) 261
BseBI CCWGG 4 cut(s) 867, 1050, 1172, 1260
BseDI CCNNGG 3 cut(s) 79, 1159, 1259
BseGI GGATG 3 cut(s) 385, 902, 1172
BseLI CCNNNNNNNGG 3 cut(s) 284, 647, 1149
BseMI GCAATG 1 cut(s) 261
BseMII CTCAG 1 cut(s) 389
BseNI ACTGG 3 cut(s) 236, 623, 1088
BseSI GKGCMC 1 cut(s) 849
BsgI GTGCAG 2 cut(s) 564, 705
Bsh1285I CGRYCG 1 cut(s) 343
BshFI GGCC 3 cut(s) 29, 591, 791
BsiEI CGRYCG 1 cut(s) 343
BsiSI CCGG 2 cut(s) 26, 768
BslFI GGGAC 1 cut(s) 1276
BslI CCNNNNNNNGG 3 cut(s) 284, 647, 1149
BsmAI GTCTC 2 cut(s) 504, 752
BsmFI GGGAC 1 cut(s) 1276
BsmI GAATGC 1 cut(s) 209
BsnI GGCC 3 cut(s) 29, 591, 791
Bso31I GGTCTC 1 cut(s) 504
Bsp1286I GDGCHC 1 cut(s) 849
Bsp143I GATC 8 cut(s) 75, 96, 298, 340, 429, 892, 1212, 1238
Bsp19I CCATGG 1 cut(s) 1159
BspACI CCGC 2 cut(s) 84, 1201
BspANI GGCC 3 cut(s) 29, 591, 791
BspCNI CTCAG 1 cut(s) 390
BspLI GGNNCC 2 cut(s) 77, 871
BspMI ACCTGC 2 cut(s) 536, 1287
BspPI GGATC 6 cut(s) 70, 83, 306, 437, 1220, 1246
BspTNI GGTCTC 1 cut(s) 504
BsrDI GCAATG 1 cut(s) 261
BsrFI RCCGGY 1 cut(s) 25
BsrI ACTGG 3 cut(s) 236, 623, 1088
BssAI RCCGGY 1 cut(s) 25
BssECI CCNNGG 3 cut(s) 79, 1159, 1259
BssMI GATC 8 cut(s) 75, 96, 298, 340, 429, 892, 1212, 1238
BssNI GRCGYC 1 cut(s) 23
BssT1I CCWWGG 2 cut(s) 79, 1159
Bst2UI CCWGG 4 cut(s) 867, 1050, 1172, 1260
Bst4CI ACNGT 7 cut(s) 17, 128, 205, 421, 482, 1029, 1138
Bst6I CTCTTC 1 cut(s) 445
BstACI GRCGYC 1 cut(s) 23
BstAPI GCANNNNNTGC 2 cut(s) 413, 658
BstBAI YACGTR 2 cut(s) 518, 1226
BstC8I GCNNGC 2 cut(s) 27, 773
BstDEI CTNAG 4 cut(s) 65, 169, 398, 578
BstDSI CCRYGG 1 cut(s) 1159
BstF5I GGATG 3 cut(s) 385, 902, 1172
BstKTI GATC 8 cut(s) 78, 99, 301, 343, 432, 895, 1215, 1241
BstMAI GTCTC 2 cut(s) 504, 752
BstMBI GATC 8 cut(s) 75, 96, 298, 340, 429, 892, 1212, 1238
BstMCI CGRYCG 1 cut(s) 343
BstMWI GCNNNNNNNGC 4 cut(s) 413, 658, 719, 956
BstNI CCWGG 4 cut(s) 867, 1050, 1172, 1260
BstNSI RCATGY 2 cut(s) 1308, 1315
BstPAI GACNNNNGTC 1 cut(s) 874
BstSCI CCNGG 4 cut(s) 865, 1048, 1170, 1258
BstSFI CTRYAG 1 cut(s) 204
BstSLI GKGCMC 1 cut(s) 849
BstX2I RGATCY 2 cut(s) 75, 1238
BstYI RGATCY 2 cut(s) 75, 1238
BsuRI GGCC 3 cut(s) 29, 591, 791
BtgI CCRYGG 1 cut(s) 1159
BtsCI GGATG 3 cut(s) 385, 902, 1172
BtsIMutI CAGTG 5 cut(s) 201, 229, 487, 1019, 1095
BveI ACCTGC 2 cut(s) 536, 1287
Cac8I GCNNGC 2 cut(s) 27, 773
Cfr10I RCCGGY 1 cut(s) 25
Cfr13I GGNCC 3 cut(s) 28, 590, 869
CseI GACGC 1 cut(s) 31
Csp6I GTAC 1 cut(s) 1315
CviAII CATG 8 cut(s) 163, 356, 413, 823, 1160, 1207, 1305, 1312
CviQI GTAC 1 cut(s) 1315
DdeI CTNAG 4 cut(s) 65, 169, 398, 578
DpnI GATC 8 cut(s) 77, 98, 300, 342, 431, 894, 1214, 1240
DpnII GATC 8 cut(s) 75, 96, 298, 340, 429, 892, 1212, 1238
Eam1104I CTCTTC 1 cut(s) 445
EarI CTCTTC 1 cut(s) 445
Eco130I CCWWGG 2 cut(s) 79, 1159
Eco147I AGGCCT 1 cut(s) 791
Eco31I GGTCTC 1 cut(s) 504
Eco47I GGWCC 1 cut(s) 869
EcoO109I RGGNCCY 1 cut(s) 869
EcoRII CCWGG 4 cut(s) 865, 1048, 1170, 1258
EcoT14I CCWWGG 2 cut(s) 79, 1159
EcoT22I ATGCAT 2 cut(s) 1111, 1306
ErhI CCWWGG 2 cut(s) 79, 1159
FaeI CATG 8 cut(s) 166, 359, 416, 826, 1163, 1210, 1308, 1315
FaqI GGGAC 1 cut(s) 1276
FatI CATG 8 cut(s) 162, 355, 412, 822, 1159, 1206, 1304, 1311
FauI CCCGC 2 cut(s) 77, 1194
FauNDI CATATG 1 cut(s) 184
FbaI TGATCA 1 cut(s) 96
FokI GGATG 3 cut(s) 372, 889, 1179
FspBI CTAG 4 cut(s) 80, 783, 807, 1055
HaeIII GGCC 3 cut(s) 29, 591, 791
HapII CCGG 2 cut(s) 26, 768
HgaI GACGC 1 cut(s) 31
Hin1I GRCGYC 1 cut(s) 23
Hin1II CATG 8 cut(s) 166, 359, 416, 826, 1163, 1210, 1308, 1315
HindIII AAGCTT 1 cut(s) 711
HinfI GANTC 3 cut(s) 471, 485, 1093
HpaII CCGG 2 cut(s) 26, 768
HphI GGTGA 5 cut(s) 140, 539, 623, 1010, 1248
Hpy166II GTNNAC 2 cut(s) 39, 631
Hpy188I TCNGA 4 cut(s) 46, 219, 1102, 1178
Hpy188III TCNNGA 8 cut(s) 117, 147, 278, 783, 890, 1040, 1055, 1130
Hpy8I GTNNAC 2 cut(s) 39, 631
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 1 cut(s) 1052
HpyCH4III ACNGT 7 cut(s) 17, 128, 205, 421, 482, 1029, 1138
HpyCH4IV ACGT 3 cut(s) 517, 645, 1225
HpyF10VI GCNNNNNNNGC 4 cut(s) 413, 658, 719, 956
HpyF3I CTNAG 4 cut(s) 65, 169, 398, 578
HpySE526I ACGT 3 cut(s) 517, 645, 1225
Hsp92I GRCGYC 1 cut(s) 23
Hsp92II CATG 8 cut(s) 166, 359, 416, 826, 1163, 1210, 1308, 1315
KroI GCCGGC 1 cut(s) 25
KroNI GCCGGC 1 cut(s) 27
Ksp22I TGATCA 1 cut(s) 96
Kzo9I GATC 8 cut(s) 75, 96, 298, 340, 429, 892, 1212, 1238
LmnI GCTCC 2 cut(s) 359, 928
LweI GCATC 5 cut(s) 241, 458, 1096, 1118, 1157
MaeI CTAG 4 cut(s) 80, 783, 807, 1055
MaeII ACGT 3 cut(s) 517, 645, 1225
MaeIII GTNAC 3 cut(s) 223, 319, 1307
MalI GATC 8 cut(s) 77, 98, 300, 342, 431, 894, 1214, 1240
MboI GATC 8 cut(s) 75, 96, 298, 340, 429, 892, 1212, 1238
MboII GAAGA 4 cut(s) 20, 59, 432, 1205
MfeI CAATTG 1 cut(s) 552
MflI RGATCY 2 cut(s) 75, 1238
MhlI GDGCHC 1 cut(s) 849
MmeI TCCRAC 2 cut(s) 836, 1156
Mph1103I ATGCAT 2 cut(s) 1111, 1306
MroNI GCCGGC 1 cut(s) 25
MseI TTAA 3 cut(s) 153, 1007, 1324
MslI CAYNNNNRTG 3 cut(s) 187, 1158, 1283
MspI CCGG 2 cut(s) 26, 768
MspR9I CCNGG 4 cut(s) 867, 1050, 1172, 1260
MunI CAATTG 1 cut(s) 552
Mva1269I GAATGC 1 cut(s) 209
MvaI CCWGG 4 cut(s) 867, 1050, 1172, 1260
MwoI GCNNNNNNNGC 4 cut(s) 413, 658, 719, 956
NaeI GCCGGC 1 cut(s) 27
NcoI CCATGG 1 cut(s) 1159
NdeI CATATG 1 cut(s) 184
NdeII GATC 8 cut(s) 75, 96, 298, 340, 429, 892, 1212, 1238
NgoMIV GCCGGC 1 cut(s) 25
NlaIII CATG 8 cut(s) 166, 359, 416, 826, 1163, 1210, 1308, 1315
NlaIV GGNNCC 2 cut(s) 77, 871
NmuCI GTSAC 1 cut(s) 319
NsiI ATGCAT 2 cut(s) 1111, 1306
NspI RCATGY 2 cut(s) 1308, 1315
PaqCI CACCTGC 1 cut(s) 536
PceI AGGCCT 1 cut(s) 791
PciI ACATGT 1 cut(s) 1311
PctI GAATGC 1 cut(s) 209
PdiI GCCGGC 1 cut(s) 27
PfeI GAWTC 3 cut(s) 471, 485, 1093
PflMI CCANNNNNTGG 2 cut(s) 284, 1149
PfoI TCCNGGA 1 cut(s) 865
Ple19I CGATCG 1 cut(s) 343
Ppu21I YACGTR 2 cut(s) 518, 1226
PpuMI RGGWCCY 1 cut(s) 869
PscI ACATGT 1 cut(s) 1311
PshAI GACNNNNGTC 1 cut(s) 874
Psp5II RGGWCCY 1 cut(s) 869
Psp6I CCWGG 4 cut(s) 865, 1048, 1170, 1258
PspGI CCWGG 4 cut(s) 865, 1048, 1170, 1258
PspN4I GGNNCC 2 cut(s) 77, 871
PspPI GGNCC 3 cut(s) 28, 590, 869
PspPPI RGGWCCY 1 cut(s) 869
PsuI RGATCY 2 cut(s) 75, 1238
PvuI CGATCG 1 cut(s) 343
RsaI GTAC 1 cut(s) 1316
RsaNI GTAC 1 cut(s) 1315
RseI CAYNNNNRTG 3 cut(s) 187, 1158, 1283
SaqAI TTAA 3 cut(s) 153, 1007, 1324
Sau3AI GATC 8 cut(s) 75, 96, 298, 340, 429, 892, 1212, 1238
Sau96I GGNCC 3 cut(s) 28, 590, 869
ScrFI CCNGG 4 cut(s) 867, 1050, 1172, 1260
SduI GDGCHC 1 cut(s) 849
SfaNI GCATC 5 cut(s) 241, 458, 1096, 1118, 1157
SfcI CTRYAG 1 cut(s) 204
SinI GGWCC 1 cut(s) 869
SmiMI CAYNNNNRTG 3 cut(s) 187, 1158, 1283
SmlI CTYRAG 3 cut(s) 115, 1038, 1272
SmoI CTYRAG 3 cut(s) 115, 1038, 1272
SseBI AGGCCT 1 cut(s) 791
SsiI CCGC 2 cut(s) 84, 1201
SspI AATATT 1 cut(s) 103
SspMI CTAG 4 cut(s) 80, 783, 807, 1055
StuI AGGCCT 1 cut(s) 791
StyD4I CCNGG 4 cut(s) 865, 1048, 1170, 1258
StyI CCWWGG 2 cut(s) 79, 1159
TaaI ACNGT 7 cut(s) 17, 128, 205, 421, 482, 1029, 1138
TaiI ACGT 3 cut(s) 520, 648, 1228
TaqI TCGA 1 cut(s) 1129
TatI WGTACW 1 cut(s) 1314
TfiI GAWTC 3 cut(s) 471, 485, 1093
Tru1I TTAA 3 cut(s) 153, 1007, 1324
Tru9I TTAA 3 cut(s) 153, 1007, 1324
TscAI CASTG 5 cut(s) 208, 236, 487, 1026, 1095
TseFI GTSAC 1 cut(s) 319
Tsp45I GTSAC 1 cut(s) 319
TspDTI ATGAA 5 cut(s) 171, 179, 201, 597, 1206
TspGWI ACGGA 2 cut(s) 88, 203
TspRI CASTG 5 cut(s) 208, 236, 487, 1026, 1095
Van91I CCANNNNNTGG 2 cut(s) 284, 1149
VpaK11BI GGWCC 1 cut(s) 869
XapI RAATTY 4 cut(s) 131, 832, 860, 935
XbaI TCTAGA 2 cut(s) 782, 1054
XceI RCATGY 2 cut(s) 1308, 1315
XmaJI CCTAGG 1 cut(s) 79
XspI CTAG 4 cut(s) 80, 783, 807, 1055
Zsp2I ATGCAT 2 cut(s) 1111, 1306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.