Rorug04G0045500

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000533
Physical Location & Seq
Forward (+)
7084065 .. 7084556
492 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0045500.1

Sequence Viewer

Length: 354 bp
ATGGAGTACTCTAAGTACGTTCACAAGTTAGGTGTCACTTTGTTTGAATTTCTATCTAAGGCGCTTGGGCTCAAACTTGATCACCTCAAAAACTTGGATAGCACGAAGGGGCATATGATTCTTAGTCATTACTATCCACCATGCCCGGAGCCTGAAGTAGCTATTGGCACTGTTGAACACTCAGATCCTGATTTTATGACCATCCTACTTCAAGACCATATTGGTGGACTTCAGGTTTTGTACCAAAATCAGTGTATTAATGTTCCTCCTGTACCTGGAGCTCTAGAGGTGAACATCGGAGATTTCTTACAGGCAAGTTTCTATATCATATATATTCACAAAGGGAATGGTTAA

Protein Analysis

117

Amino Acids

13.1

Weight (kDa)

5.7

Isoelectric Point (pI)

47.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 38 - 105 8.2e-20 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 179
AcsI RAATTY 1 cut(s) 47
AcuI CTGAAG 2 cut(s) 174, 215
AfaI GTAC 4 cut(s) 8, 17, 242, 273
AfiI CCNNNNNNNGG 1 cut(s) 275
AgsI TTSAA 3 cut(s) 47, 176, 212
AjnI CCWGG 1 cut(s) 274
AjuI GAANNNNNNNTTGG 2 cut(s) 147, 179
AluBI AGCT 2 cut(s) 161, 281
AluI AGCT 2 cut(s) 161, 281
Alw21I GWGCWC 1 cut(s) 283
AlwI GGATC 1 cut(s) 179
AlwNI CAGNNNCTG 1 cut(s) 188
ApoI RAATTY 1 cut(s) 47
AseI ATTAAT 1 cut(s) 258
AspLEI GCGC 1 cut(s) 64
AsuC2I CCSGG 1 cut(s) 146
AsuHPI GGTGA 2 cut(s) 74, 301
BanII GRGCYC 2 cut(s) 72, 283
Bbv12I GWGCWC 1 cut(s) 283
BccI CCATC 1 cut(s) 209
BciT130I CCWGG 1 cut(s) 276
BclI TGATCA 1 cut(s) 79
BcnI CCSGG 1 cut(s) 146
BfaI CTAG 1 cut(s) 284
BfoI RGCGCY 1 cut(s) 65
BmcAI AGTACT 1 cut(s) 8
Bme1390I CCNGG 2 cut(s) 146, 276
BmiI GGNNCC 1 cut(s) 150
BmrFI CCNGG 2 cut(s) 146, 276
BpmI CTGGAG 1 cut(s) 297
BpuMI CCSGG 1 cut(s) 146
Bsc4I CCNNNNNNNGG 1 cut(s) 275
BseBI CCWGG 1 cut(s) 276
BseGI GGATG 1 cut(s) 201
BseLI CCNNNNNNNGG 1 cut(s) 275
BseMII CTCAG 1 cut(s) 195
BsiHKAI GWGCWC 1 cut(s) 283
BsiSI CCGG 1 cut(s) 146
BslI CCNNNNNNNGG 1 cut(s) 275
Bsp1286I GDGCHC 2 cut(s) 72, 283
Bsp143I GATC 2 cut(s) 79, 184
BspCNI CTCAG 1 cut(s) 194
BspLI GGNNCC 1 cut(s) 150
BspPI GGATC 1 cut(s) 179
BssMI GATC 2 cut(s) 79, 184
Bst2UI CCWGG 1 cut(s) 276
Bst4CI ACNGT 1 cut(s) 172
BstDEI CTNAG 4 cut(s) 12, 57, 122, 181
BstF5I GGATG 1 cut(s) 201
BstH2I RGCGCY 1 cut(s) 65
BstHHI GCGC 1 cut(s) 64
BstKTI GATC 2 cut(s) 82, 187
BstMBI GATC 2 cut(s) 79, 184
BstNI CCWGG 1 cut(s) 276
BstSCI CCNGG 2 cut(s) 144, 274
BstX2I RGATCY 1 cut(s) 184
BstXI CCANNNNNNTGG 1 cut(s) 224
BstYI RGATCY 1 cut(s) 184
BtsCI GGATG 1 cut(s) 201
BtsIMutI CAGTG 2 cut(s) 168, 257
CaiI CAGNNNCTG 1 cut(s) 188
CfoI GCGC 1 cut(s) 64
Csp6I GTAC 4 cut(s) 7, 16, 241, 272
CviAII CATG 1 cut(s) 141
CviJI RGCY 4 cut(s) 70, 151, 161, 281
CviKI_1 RGCY 4 cut(s) 70, 151, 161, 281
CviQI GTAC 4 cut(s) 7, 16, 241, 272
DdeI CTNAG 4 cut(s) 12, 57, 122, 181
DpnI GATC 2 cut(s) 81, 186
DpnII GATC 2 cut(s) 79, 184
Ecl136II GAGCTC 1 cut(s) 281
Eco24I GRGCYC 2 cut(s) 72, 283
Eco53kI GAGCTC 1 cut(s) 281
Eco57I CTGAAG 2 cut(s) 174, 215
EcoICRI GAGCTC 1 cut(s) 281
EcoRII CCWGG 1 cut(s) 274
EcoT38I GRGCYC 2 cut(s) 72, 283
FaeI CATG 1 cut(s) 144
FaiI YATR 9 cut(s) 114, 116, 142, 197, 219, 324, 329, 331, 333
FatI CATG 1 cut(s) 140
FauNDI CATATG 1 cut(s) 114
FbaI TGATCA 1 cut(s) 79
FokI GGATG 1 cut(s) 188
FriOI GRGCYC 2 cut(s) 72, 283
FspBI CTAG 1 cut(s) 284
GlaI GCGC 1 cut(s) 63
GsuI CTGGAG 1 cut(s) 297
HaeII RGCGCY 1 cut(s) 65
HapII CCGG 1 cut(s) 146
HhaI GCGC 1 cut(s) 64
Hin1II CATG 1 cut(s) 144
Hin6I GCGC 1 cut(s) 62
HinP1I GCGC 1 cut(s) 62
HinfI GANTC 1 cut(s) 118
HpaII CCGG 1 cut(s) 146
HphI GGTGA 2 cut(s) 74, 301
Hpy166II GTNNAC 3 cut(s) 22, 227, 292
Hpy188I TCNGA 2 cut(s) 184, 299
Hpy188III TCNNGA 3 cut(s) 188, 212, 284
Hpy8I GTNNAC 3 cut(s) 22, 227, 292
HpyAV CCTTC 1 cut(s) 100
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4IV ACGT 1 cut(s) 18
HpyF3I CTNAG 4 cut(s) 12, 57, 122, 181
HpySE526I ACGT 1 cut(s) 18
Hsp92II CATG 1 cut(s) 144
HspAI GCGC 1 cut(s) 62
Ksp22I TGATCA 1 cut(s) 79
Kzo9I GATC 2 cut(s) 79, 184
LmnI GCTCC 2 cut(s) 148, 278
LpnPI CCDG 8 cut(s) 159, 165, 201, 218, 261, 282, 288, 296
MaeI CTAG 1 cut(s) 284
MaeII ACGT 1 cut(s) 18
MaeIII GTNAC 1 cut(s) 34
MalI GATC 2 cut(s) 81, 186
MboI GATC 2 cut(s) 79, 184
MflI RGATCY 1 cut(s) 184
MhlI GDGCHC 2 cut(s) 72, 283
MluCI AATT 1 cut(s) 47
MnlI CCTC 3 cut(s) 95, 276, 280
MseI TTAA 2 cut(s) 258, 352
MslI CAYNNNNRTG 1 cut(s) 222
MspI CCGG 1 cut(s) 146
MspR9I CCNGG 2 cut(s) 146, 276
MvaI CCWGG 1 cut(s) 276
NciI CCSGG 1 cut(s) 146
NdeI CATATG 1 cut(s) 114
NdeII GATC 2 cut(s) 79, 184
NlaIII CATG 1 cut(s) 144
NlaIV GGNNCC 1 cut(s) 150
NmuCI GTSAC 1 cut(s) 34
PfeI GAWTC 1 cut(s) 118
PshBI ATTAAT 1 cut(s) 258
Psp124BI GAGCTC 1 cut(s) 283
Psp6I CCWGG 1 cut(s) 274
PspGI CCWGG 1 cut(s) 274
PspN4I GGNNCC 1 cut(s) 150
PstNI CAGNNNCTG 1 cut(s) 188
PsuI RGATCY 1 cut(s) 184
RsaI GTAC 4 cut(s) 8, 17, 242, 273
RsaNI GTAC 4 cut(s) 7, 16, 241, 272
RseI CAYNNNNRTG 1 cut(s) 222
SacI GAGCTC 1 cut(s) 283
SaqAI TTAA 2 cut(s) 258, 352
Sau3AI GATC 2 cut(s) 79, 184
ScaI AGTACT 1 cut(s) 8
ScrFI CCNGG 2 cut(s) 146, 276
SduI GDGCHC 2 cut(s) 72, 283
SetI ASST 8 cut(s) 21, 34, 87, 163, 237, 277, 283, 291
SmiMI CAYNNNNRTG 1 cut(s) 222
Sse9I AATT 1 cut(s) 47
SspMI CTAG 1 cut(s) 284
SstI GAGCTC 1 cut(s) 283
StyD4I CCNGG 2 cut(s) 144, 274
TaaI ACNGT 1 cut(s) 172
TaiI ACGT 1 cut(s) 21
TasI AATT 1 cut(s) 47
TatI WGTACW 1 cut(s) 6
TfiI GAWTC 1 cut(s) 118
Tru1I TTAA 2 cut(s) 258, 352
Tru9I TTAA 2 cut(s) 258, 352
TscAI CASTG 2 cut(s) 175, 257
TseFI GTSAC 1 cut(s) 34
Tsp45I GTSAC 1 cut(s) 34
TspRI CASTG 2 cut(s) 175, 257
VspI ATTAAT 1 cut(s) 258
XapI RAATTY 1 cut(s) 47
XbaI TCTAGA 1 cut(s) 283
XspI CTAG 1 cut(s) 284
ZrmI AGTACT 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.