Prupe.1G092500_v2.0.a1

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000533
Physical Location & Seq
Reverse (-)
7027824 .. 7030070
2247 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G092500.1

Sequence Viewer

Length: 1731 bp
ATGGTGAAATCAACAATGTCAGCTATACTAGTATCGGTGCTGCACCTTTTTGTTCTTCATCTTCAATATTCAGAGGTTCATTCGCTTGCCAATACCTCTGCTCATGATTTTAGCTACTTGGAATTTGTTTACGATGCCAATGACACAGAGTTGGAAGGAACATACGACTACATTATTGTTGGTGGAGGAACAGCAGGGTGTCCATTGGCAGCAACTTTGTCAGCAAACTACTCGGTGCTTGTTCTGGAAAGGGGCACTCTTCCTACAGAATATCCAAACCTGTTGACTTCAGACGGGTTTATATATAATCTGCAGCAAGAAGATGATGGACAGACACCAGTCGAAAGGTTCGTGTCTGGAGATGGTATTGACAATGTAAGAGGGAGGGTCCTCGGTGGCACGAGCATGATCAATGCCGGGGTCTACGTAAGAGCTAACACCTCGTTCTTTAATCAAACAGGGATTGAATGGGATATGGATCTGGTTAATAAGACATATGACTGGGTTGAAGACACTATTGTGTTCAAGCCGGATTTCCAATTTTGGCAAAATCTTACAGGAACTGCGTTCTTGGAGGTTGGTATTCTTCCAGACAATGGATTTAGTTTGGATCACATAGAAGGAACTAGACTCACCGGCTCAACTTTCGACAATAACGGAACCAGACATGCATCTGATGAACTGCTTAATAAAGGAGACCCAAACAACTTGAAAGTTGCAGTTCATGCCGCAGTAGAGAAGATCATCTTCTCTTCCAATTCATCAGGTGTGACAGCTATAGGAGTAATATATACTGATTCGAACGGAACGACTCATCAGGCATTTGTACGTGTTGAGGGAGAAGTTATATTGAGTGCAGGGCCAATTGGGTCCCCTCAACTTCTACTACTTAGTGGTGTTGGCCCAGAGTCTTACCTAACATCTCTCAACATTTCAGTTGTTGCTTCCCATCCATACGTCGGGCAGTATATATATGACAATCCTCGTAATTTCATTAACATTTTGCCCCCAAATCCAATAGAACCCTCAACTGTAACCGTTCTAGGCATTACAAGCGACTTCTACCAATGTTCTCTATCAAGCTTGCCATTTTCTATTGCACCCTTTAGTTTTTTTCCTATTCCAACTTATCCCCTGCCAAATACGACTTTCGCTCACATTGTTAACAAAGTTCCGGGACCATTATCTCACGGTACTGTCACGCTGCAATCAACCTCTGATGTGAGAGTCGCTCCAAATGTCACATTCAACTACTATTCGAATTCAACTGACCTTGCTCATTGTGTTAGCGGCATGAAGAAGATTGGTGAATTCTTGAGCTCAGACGCATTAAAACCATATAAAGTGGAAGATTTGCCGGGCATAGAAGGTTTTGATATTTTGGGAATACCTTTGCCAGAGAACCAGACAGATGATGCAGCCTTCGAAACATTTTGCCAAGAGGCAGTAGCGTCATACTGGCATTACCACGGTGGATGCCTTGTCGGGGAGGTGCTTGATGATGATTTCCGTGTTACAGGGATCAACGCATTGCGTGTTGTTGATGGCTCCACCTTCCCTTCCACACCAGCGAGCCATCCACAGGGCTTCTATCTGATGTTAGGGAGGTACGTGGGCTCTAAAATTTTGCAAGAAAGATTAGCTTCAGAGGAGGCTCTTCATAAGTCAACAATCCAACCCAAAATCTTGGAGTCGCTTGAGTCAGCATTATCCTTTGCGTTTGATACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko01110 map00460 map01110
Pfam Domains
Protein Families
GMC_oxred_C GMC_oxred_N

Protein Analysis

577

Amino Acids

62.68

Weight (kDa)

4.58

Isoelectric Point (pI)

34.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 596
AccI GTMKAC 1 cut(s) 423
AciI CCGC 2 cut(s) 729, 1290
AclWI GGATC 3 cut(s) 486, 618, 1529
AcsI RAATTY 4 cut(s) 122, 1261, 1310, 1623
AcuI CTGAAG 2 cut(s) 273, 1629
AfaI GTAC 3 cut(s) 828, 1195, 1610
AfiI CCNNNNNNNGG 4 cut(s) 596, 959, 1486, 1582
AflIII ACRYGT 1 cut(s) 829
AgsI TTSAA 7 cut(s) 65, 467, 509, 526, 712, 1249, 1266
AhlI ACTAGT 1 cut(s) 28
AleI CACNNNNGTG 1 cut(s) 518
AluBI AGCT 7 cut(s) 23, 114, 434, 776, 1083, 1320, 1643
AluI AGCT 7 cut(s) 23, 114, 434, 776, 1083, 1320, 1643
Alw21I GWGCWC 1 cut(s) 1322
Alw26I GTCTC 1 cut(s) 690
AlwI GGATC 3 cut(s) 486, 618, 1529
AlwNI CAGNNNCTG 1 cut(s) 563
AoxI GGCC 2 cut(s) 860, 901
ApeKI GCWGC 5 cut(s) 40, 209, 313, 1204, 1418
ApoI RAATTY 4 cut(s) 122, 1261, 1310, 1623
AspS9I GGNCC 5 cut(s) 388, 860, 870, 902, 1178
AsuC2I CCSGG 3 cut(s) 418, 1176, 1359
AsuHPI GGTGA 3 cut(s) 16, 625, 1319
AsuII TTCGAA 3 cut(s) 800, 1259, 1425
AvaII GGWCC 3 cut(s) 388, 870, 1178
BaeGI GKGCMC 1 cut(s) 257
BanII GRGCYC 2 cut(s) 1322, 1619
BauI CACGAG 1 cut(s) 400
BbsI GAAGAC 1 cut(s) 516
Bbv12I GWGCWC 1 cut(s) 1322
BbvI GCAGC 5 cut(s) 27, 221, 325, 1191, 1430
BccI CCATC 5 cut(s) 320, 356, 957, 1538, 1584
BcgI CGANNNNNNTGC 2 cut(s) 213, 247
BclI TGATCA 1 cut(s) 408
BcnI CCSGG 3 cut(s) 418, 1176, 1359
BcoDI GTCTC 1 cut(s) 690
BcuI ACTAGT 1 cut(s) 28
BfaI CTAG 3 cut(s) 29, 627, 1043
BfmI CTRYAG 3 cut(s) 264, 311, 777
BisI GCNGC 7 cut(s) 41, 210, 314, 729, 1205, 1291, 1419
BlsI GCNGC 7 cut(s) 42, 211, 315, 730, 1206, 1292, 1420
Bme1390I CCNGG 3 cut(s) 418, 1176, 1359
Bme18I GGWCC 3 cut(s) 388, 870, 1178
BmgT120I GGNCC 5 cut(s) 388, 860, 870, 902, 1178
BmiI GGNNCC 6 cut(s) 389, 661, 871, 872, 1179, 1549
BmrFI CCNGG 3 cut(s) 418, 1176, 1359
BmrI ACTGGG 1 cut(s) 511
BmsI GCATC 4 cut(s) 124, 680, 1405, 1466
BmuI ACTGGG 1 cut(s) 511
BoxI GACNNNNGTC 1 cut(s) 338
BpiI GAAGAC 1 cut(s) 516
BplI GAGNNNNNCTC 2 cut(s) 1216, 1248
BpmI CTGGAG 1 cut(s) 378
Bpu14I TTCGAA 3 cut(s) 800, 1259, 1425
BpuEI CTTGAG 2 cut(s) 1336, 1718
BpuMI CCSGG 3 cut(s) 418, 1176, 1359
BsaAI YACGTR 3 cut(s) 427, 830, 1612
BsaBI GATNNNNATC 1 cut(s) 477
BsaI GGTCTC 1 cut(s) 690
BsaJI CCNNGG 3 cut(s) 391, 417, 1468
Bsc4I CCNNNNNNNGG 4 cut(s) 596, 959, 1486, 1582
Bse118I RCCGGY 1 cut(s) 635
Bse1I ACTGG 3 cut(s) 338, 506, 1463
Bse3DI GCAATG 1 cut(s) 1529
Bse8I GATNNNNATC 1 cut(s) 477
BseDI CCNNGG 3 cut(s) 391, 417, 1468
BseGI GGATG 3 cut(s) 949, 1481, 1576
BseJI GATNNNNATC 1 cut(s) 477
BseLI CCNNNNNNNGG 4 cut(s) 596, 959, 1486, 1582
BseMI GCAATG 1 cut(s) 1529
BseMII CTCAG 1 cut(s) 1335
BseNI ACTGG 3 cut(s) 338, 506, 1463
BseRI GAGGAG 1 cut(s) 1664
BseSI GKGCMC 1 cut(s) 257
BseXI GCAGC 5 cut(s) 27, 221, 325, 1191, 1430
BsgI GTGCAG 2 cut(s) 26, 876
BshFI GGCC 2 cut(s) 862, 903
BsiHKAI GWGCWC 1 cut(s) 1322
BsiSI CCGG 5 cut(s) 417, 530, 636, 1175, 1358
BslFI GGGAC 2 cut(s) 856, 1191
BslI CCNNNNNNNGG 4 cut(s) 596, 959, 1486, 1582
BsmAI GTCTC 1 cut(s) 690
BsmFI GGGAC 2 cut(s) 856, 1191
BsnI GGCC 2 cut(s) 862, 903
Bso31I GGTCTC 1 cut(s) 690
Bsp119I TTCGAA 3 cut(s) 800, 1259, 1425
Bsp1286I GDGCHC 3 cut(s) 257, 1322, 1619
Bsp143I GATC 5 cut(s) 408, 478, 610, 741, 1521
BspACI CCGC 2 cut(s) 729, 1290
BspANI GGCC 2 cut(s) 862, 903
BspCNI CTCAG 1 cut(s) 1334
BspHI TCATGA 1 cut(s) 103
BspLI GGNNCC 6 cut(s) 389, 661, 871, 872, 1179, 1549
BspMAI CTGCAG 1 cut(s) 315
BspPI GGATC 3 cut(s) 486, 618, 1529
BspQI GCTCTTC 1 cut(s) 1662
BspT104I TTCGAA 3 cut(s) 800, 1259, 1425
BspTNI GGTCTC 1 cut(s) 690
BsrDI GCAATG 1 cut(s) 1529
BsrFI RCCGGY 1 cut(s) 635
BsrI ACTGG 3 cut(s) 338, 506, 1463
BssAI RCCGGY 1 cut(s) 635
BssECI CCNNGG 3 cut(s) 391, 417, 1468
BssMI GATC 5 cut(s) 408, 478, 610, 741, 1521
BssSI CACGAG 1 cut(s) 400
Bst2BI CACGAG 1 cut(s) 400
Bst4CI ACNGT 5 cut(s) 1033, 1039, 1193, 1198, 1472
Bst6I CTCTTC 3 cut(s) 264, 757, 1662
BstAPI GCANNNNNTGC 1 cut(s) 725
BstBAI YACGTR 3 cut(s) 427, 830, 1612
BstBI TTCGAA 3 cut(s) 800, 1259, 1425
BstC8I GCNNGC 3 cut(s) 87, 1085, 1573
BstDEI CTNAG 3 cut(s) 890, 1321, 1728
BstDSI CCRYGG 1 cut(s) 1468
BstF5I GGATG 3 cut(s) 949, 1481, 1576
BstKTI GATC 5 cut(s) 411, 481, 613, 744, 1524
BstMAI GTCTC 1 cut(s) 690
BstMBI GATC 5 cut(s) 408, 478, 610, 741, 1521
BstMWI GCNNNNNNNGC 2 cut(s) 725, 1053
BstNSI RCATGY 1 cut(s) 671
BstPAI GACNNNNGTC 1 cut(s) 338
BstSCI CCNGG 3 cut(s) 416, 1174, 1357
BstSFI CTRYAG 3 cut(s) 264, 311, 777
BstSLI GKGCMC 1 cut(s) 257
BstSNI TACGTA 1 cut(s) 427
BstV1I GCAGC 5 cut(s) 27, 221, 325, 1191, 1430
BstV2I GAAGAC 1 cut(s) 516
BstX2I RGATCY 1 cut(s) 478
BstXI CCANNNNNNTGG 1 cut(s) 1687
BstYI RGATCY 1 cut(s) 478
BsuRI GGCC 2 cut(s) 862, 903
BtgI CCRYGG 1 cut(s) 1468
BtsCI GGATG 3 cut(s) 949, 1481, 1576
Cac8I GCNNGC 3 cut(s) 87, 1085, 1573
CaiI CAGNNNCTG 1 cut(s) 563
CciI TCATGA 1 cut(s) 103
Cfr10I RCCGGY 1 cut(s) 635
Cfr13I GGNCC 5 cut(s) 388, 860, 870, 902, 1178
CseI GACGC 2 cut(s) 1334, 1440
Csp6I GTAC 3 cut(s) 827, 1194, 1609
CviAII CATG 5 cut(s) 104, 406, 668, 725, 1294
CviQI GTAC 3 cut(s) 827, 1194, 1609
DdeI CTNAG 3 cut(s) 890, 1321, 1728
DpnI GATC 5 cut(s) 410, 480, 612, 743, 1523
DpnII GATC 5 cut(s) 408, 478, 610, 741, 1521
Eam1104I CTCTTC 3 cut(s) 264, 757, 1662
EarI CTCTTC 3 cut(s) 264, 757, 1662
Ecl136II GAGCTC 1 cut(s) 1320
Eco105I TACGTA 1 cut(s) 427
Eco24I GRGCYC 2 cut(s) 1322, 1619
Eco31I GGTCTC 1 cut(s) 690
Eco47I GGWCC 3 cut(s) 388, 870, 1178
Eco53kI GAGCTC 1 cut(s) 1320
Eco57I CTGAAG 2 cut(s) 273, 1629
EcoICRI GAGCTC 1 cut(s) 1320
EcoO109I RGGNCCY 2 cut(s) 388, 870
EcoRI GAATTC 2 cut(s) 1261, 1310
EcoT22I ATGCAT 1 cut(s) 673
EcoT38I GRGCYC 2 cut(s) 1322, 1619
FaeI CATG 5 cut(s) 107, 409, 671, 728, 1297
FalI AAGNNNNNCTT 4 cut(s) 731, 763, 1627, 1659
FaqI GGGAC 2 cut(s) 856, 1191
FatI CATG 5 cut(s) 103, 405, 667, 724, 1293
FauNDI CATATG 1 cut(s) 496
FbaI TGATCA 1 cut(s) 408
FblI GTMKAC 1 cut(s) 423
Fnu4HI GCNGC 7 cut(s) 41, 210, 314, 729, 1205, 1291, 1419
FokI GGATG 3 cut(s) 936, 1488, 1563
FriOI GRGCYC 2 cut(s) 1322, 1619
Fsp4HI GCNGC 7 cut(s) 41, 210, 314, 729, 1205, 1291, 1419
FspBI CTAG 3 cut(s) 29, 627, 1043
GluI GCNGC 7 cut(s) 41, 210, 314, 729, 1205, 1291, 1419
GsuI CTGGAG 1 cut(s) 378
HaeIII GGCC 2 cut(s) 862, 903
HapII CCGG 5 cut(s) 417, 530, 636, 1175, 1358
HgaI GACGC 2 cut(s) 1334, 1440
Hin1II CATG 5 cut(s) 107, 409, 671, 728, 1297
HincII GTYRAC 3 cut(s) 285, 1165, 1668
HindII GTYRAC 3 cut(s) 285, 1165, 1668
HindIII AAGCTT 1 cut(s) 1081
HinfI GANTC 7 cut(s) 630, 797, 811, 908, 1227, 1691, 1700
HpaI GTTAAC 1 cut(s) 1165
HpaII CCGG 5 cut(s) 417, 530, 636, 1175, 1358
HphI GGTGA 3 cut(s) 16, 625, 1319
Hpy166II GTNNAC 5 cut(s) 130, 285, 424, 1165, 1668
Hpy188I TCNGA 7 cut(s) 73, 292, 676, 1219, 1324, 1596, 1648
Hpy188III TCNNGA 5 cut(s) 104, 245, 357, 590, 1315
Hpy8I GTNNAC 5 cut(s) 130, 285, 424, 1165, 1668
Hpy99I CGWCG 1 cut(s) 962
HpyAV CCTTC 6 cut(s) 149, 614, 1361, 1432, 1564, 1569
HpyCH4III ACNGT 5 cut(s) 1033, 1039, 1193, 1198, 1472
HpyCH4IV ACGT 4 cut(s) 426, 829, 957, 1611
HpyCH4V TGCA 9 cut(s) 43, 313, 671, 719, 857, 1100, 1207, 1418, 1630
HpyF10VI GCNNNNNNNGC 2 cut(s) 725, 1053
HpyF3I CTNAG 3 cut(s) 890, 1321, 1728
HpySE526I ACGT 4 cut(s) 426, 829, 957, 1611
Hsp92II CATG 5 cut(s) 107, 409, 671, 728, 1297
KflI GGGWCCC 1 cut(s) 870
Ksp22I TGATCA 1 cut(s) 408
KspAI GTTAAC 1 cut(s) 1165
Kzo9I GATC 5 cut(s) 408, 478, 610, 741, 1521
LguI GCTCTTC 1 cut(s) 1662
LmnI GCTCC 2 cut(s) 1237, 1553
Lsp1109I GCAGC 5 cut(s) 27, 221, 325, 1191, 1430
LweI GCATC 4 cut(s) 124, 680, 1405, 1466
MaeI CTAG 3 cut(s) 29, 627, 1043
MaeII ACGT 4 cut(s) 426, 829, 957, 1611
MaeIII GTNAC 5 cut(s) 769, 1033, 1198, 1240, 1513
MalI GATC 5 cut(s) 410, 480, 612, 743, 1523
MboI GATC 5 cut(s) 408, 478, 610, 741, 1521
MfeI CAATTG 1 cut(s) 864
MflI RGATCY 1 cut(s) 478
MhlI GDGCHC 3 cut(s) 257, 1322, 1619
MluCI AATT 8 cut(s) 122, 539, 757, 864, 988, 1261, 1310, 1623
MlyI GAGTC 6 cut(s) 624, 805, 917, 1236, 1700, 1709
MmeI TCCRAC 3 cut(s) 132, 1148, 1699
Mph1103I ATGCAT 1 cut(s) 673
MseI TTAA 6 cut(s) 450, 486, 687, 996, 1164, 1331
MslI CAYNNNNRTG 2 cut(s) 404, 518
MspI CCGG 5 cut(s) 417, 530, 636, 1175, 1358
MspR9I CCNGG 3 cut(s) 418, 1176, 1359
MunI CAATTG 1 cut(s) 864
MwoI GCNNNNNNNGC 2 cut(s) 725, 1053
NciI CCSGG 3 cut(s) 418, 1176, 1359
NdeI CATATG 1 cut(s) 496
NdeII GATC 5 cut(s) 408, 478, 610, 741, 1521
NlaIII CATG 5 cut(s) 107, 409, 671, 728, 1297
NlaIV GGNNCC 6 cut(s) 389, 661, 871, 872, 1179, 1549
NmuCI GTSAC 3 cut(s) 769, 1198, 1240
NsiI ATGCAT 1 cut(s) 673
NspI RCATGY 1 cut(s) 671
NspV TTCGAA 3 cut(s) 800, 1259, 1425
OliI CACNNNNGTG 1 cut(s) 518
PagI TCATGA 1 cut(s) 103
PciSI GCTCTTC 1 cut(s) 1662
PcsI WCGNNNNNNNCGW 2 cut(s) 348, 806
PfeI GAWTC 1 cut(s) 797
PflMI CCANNNNNTGG 1 cut(s) 596
PfoI TCCNGGA 1 cut(s) 1174
PkrI GCNGC 7 cut(s) 42, 211, 315, 730, 1206, 1292, 1420
PleI GAGTC 6 cut(s) 624, 805, 916, 1235, 1699, 1708
PpsI GAGTC 6 cut(s) 624, 805, 916, 1235, 1699, 1708
Ppu21I YACGTR 3 cut(s) 427, 830, 1612
PpuMI RGGWCCY 2 cut(s) 388, 870
PshAI GACNNNNGTC 1 cut(s) 338
Psp124BI GAGCTC 1 cut(s) 1322
Psp5II RGGWCCY 2 cut(s) 388, 870
PspN4I GGNNCC 6 cut(s) 389, 661, 871, 872, 1179, 1549
PspPI GGNCC 5 cut(s) 388, 860, 870, 902, 1178
PspPPI RGGWCCY 2 cut(s) 388, 870
PstI CTGCAG 1 cut(s) 315
PstNI CAGNNNCTG 1 cut(s) 563
PsuI RGATCY 1 cut(s) 478
RsaI GTAC 3 cut(s) 828, 1195, 1610
RsaNI GTAC 3 cut(s) 827, 1194, 1609
RseI CAYNNNNRTG 2 cut(s) 404, 518
SacI GAGCTC 1 cut(s) 1322
SapI GCTCTTC 1 cut(s) 1662
SaqAI TTAA 6 cut(s) 450, 486, 687, 996, 1164, 1331
SatI GCNGC 7 cut(s) 41, 210, 314, 729, 1205, 1291, 1419
Sau3AI GATC 5 cut(s) 408, 478, 610, 741, 1521
Sau96I GGNCC 5 cut(s) 388, 860, 870, 902, 1178
SchI GAGTC 6 cut(s) 624, 805, 917, 1236, 1700, 1709
ScrFI CCNGG 3 cut(s) 418, 1176, 1359
SduI GDGCHC 3 cut(s) 257, 1322, 1619
SfaNI GCATC 4 cut(s) 124, 680, 1405, 1466
SfcI CTRYAG 3 cut(s) 264, 311, 777
SfuI TTCGAA 3 cut(s) 800, 1259, 1425
SinI GGWCC 3 cut(s) 388, 870, 1178
SmiMI CAYNNNNRTG 2 cut(s) 404, 518
SmlI CTYRAG 2 cut(s) 1315, 1697
SmoI CTYRAG 2 cut(s) 1315, 1697
SnaBI TACGTA 1 cut(s) 427
SpeI ACTAGT 1 cut(s) 28
Sse9I AATT 8 cut(s) 122, 539, 757, 864, 988, 1261, 1310, 1623
SsiI CCGC 2 cut(s) 729, 1290
SspI AATATT 1 cut(s) 68
SspMI CTAG 3 cut(s) 29, 627, 1043
SstI GAGCTC 1 cut(s) 1322
StyD4I CCNGG 3 cut(s) 416, 1174, 1357
TaaI ACNGT 5 cut(s) 1033, 1039, 1193, 1198, 1472
TaiI ACGT 4 cut(s) 429, 832, 960, 1614
TaqI TCGA 5 cut(s) 342, 648, 800, 1259, 1425
TasI AATT 8 cut(s) 122, 539, 757, 864, 988, 1261, 1310, 1623
TauI GCSGC 2 cut(s) 731, 1293
TfiI GAWTC 1 cut(s) 797
Tru1I TTAA 6 cut(s) 450, 486, 687, 996, 1164, 1331
Tru9I TTAA 6 cut(s) 450, 486, 687, 996, 1164, 1331
TseFI GTSAC 3 cut(s) 769, 1198, 1240
TseI GCWGC 5 cut(s) 40, 209, 313, 1204, 1418
Tsp45I GTSAC 3 cut(s) 769, 1198, 1240
TspDTI ATGAA 8 cut(s) 47, 68, 693, 713, 750, 982, 1310, 1649
TspGWI ACGGA 3 cut(s) 672, 819, 1499
Van91I CCANNNNNTGG 1 cut(s) 596
VpaK11BI GGWCC 3 cut(s) 388, 870, 1178
XapI RAATTY 4 cut(s) 122, 1261, 1310, 1623
XceI RCATGY 1 cut(s) 671
XmiI GTMKAC 1 cut(s) 423
XspI CTAG 3 cut(s) 29, 627, 1043
Zsp2I ATGCAT 1 cut(s) 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.