Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0000533

Physical Location & Seq

Genomic Coordinates Forward (+)

34109782 .. 34112236

Gene Structure 2455 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1264200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1659 bp

ATGGTGAAATCAATGGTGTTTCGTGTGCTATTGGTGTTGCAACTTTGTGTCCATCTTCAATCAGAGGTTCAATCGTTGGCCACTCCTTCTGAGCATGATTTTAGCTACTCAAAATCTGTGGTTAATGCCACTGATCTTCCACAAGAAGAAGTATATGACTACATCGTAGTTGGAGGGGGTACCGCAGGGTGTCCATTGGCAGCAACTTTATCATTGAACTATTCGGTGCTCGTTTTAGAAAGGGGAAGTTTTCCTGCATCATATCCGAATGTCTTGACTCAAGATGGGTTTATTTATAATCTCCAGCAAGAAGATGATGGAGAGACACCAGTTCAAAGGGTCATGTCAGAAGATGGTATTCCTACTGTTAGAGGAAGGATCCTCGGCGGCACGAGCATAATCAATGCTGGCGTTTACGCAAGAGCTAACATTTCATTCTTTAGTCAATCAGGAGTTGAATGGAACATGGATTTGGTTAATGCAACATATGAGTGGATCGAAGACACTATTGTGTACAAGCCAAATGCATTCGCTTGGCAAACTATTACACAACAAGCATTTTTGGAGGCTGGTGTTTTACCAGACAATGGATTCAGTTTGGATCACGTACTGGGAACTAGAATCACGGGCTCAACTTTTGACAATAATGGAACGAGACATGCAGCTGATGAACTACTTAACAAAGGAGACCTAGACAACTTGCGAGTTGCGGTTCATGCCAACGTAGAGAAGATCCTCATCTCTTCTACTTTTGAATCAAATTTGTCAGCAAGAGGAGTCATATTTAAGGATTCTAATGGGATTTCTCATCGGTCATACGTACGCAATCAAGGAGAAGTTATATTGAGTGCAGGGACAATGGGGACTCCTCAACTTCTACTACTTAGTGGTGTTGGCCCGGAGTCCTACTTATCATCGCTTGGAATCCCAGTTGTTATTGACCATCCTTACGTTGGTCATTTTCTGTACGACAATCCTCGTAATTTCATAAACATTTTGCCCCCAAATCCAGTTGAAGCCTCAATTGTAACAGCTCTGGGCATTAGAAACAATTTCTGGCAATGTTCTATCTCGGGTGGGCCACTTACTGTTCCACCCTACAGTTTTTTCCCTAGTCAGTCTTACCCCCTACCAAATTCGACTTTCCTTCACATTCCTAACAAAGTTCCAGGACCTTTATCTCATGGTTCTCTCACGCTAAATTCATCTAATGATGTGACTGTTGGCCCAAACGTTGTATTCAATTACTATTCCAATGCCACAGACCTTGCTCATTGTGTTAGCGGCATGAAACAGTTCAGTGATTTATTAAGGACCGATGCATTTAAACCATATAAAACCGAAGATTTGCCAGGTGTAGAAGGCTTCACCTTTTTAGGAGTTCCTTTTCCAAATAACCAAACAGATGATGCACTCGAAACATTTTGTCATGATTCACTGGCCTCATATTGGCATTACCACGGTGGATGCATTGTTGGAAAGGTGGTTGACGGTGGTTTGCGTGTTAGTGGGATCGATGCATTACGCGTTGTTGATTCTTCCACATTCCCTGTCACACCGGCGAGCCACCCTATGGGCTTCTATCTGATGTTAGGCAGGTATATGGGTCTTCAAATTCTGCAAGAGAGATCACCTTGGATTGGTATATCATCCGCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0031410 GO:0031982 GO:0033095 GO:0043226 GO:0043227 GO:0043229 GO:0044424 GO:0044444 GO:0044464 GO:0097708

KEGG Pathways

Metabolic & Signaling

ko00460 ko01110 map00460 map01110

Pfam Domains

Protein Families

GMC_oxred_C GMC_oxred_N

Protein Analysis

553

Amino Acids

60.12

Weight (kDa)

5.02

Isoelectric Point (pI)

42.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GMC_oxred_N	PF00732	52 - 324	8.6e-19	GMC oxidoreductase
GMC_oxred_C	PF05199	392 - 532	1.3e-23	GMC oxidoreductase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000533)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g08950 FvH4_6g09340
malus_domestica	MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica	Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis	pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis	RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata	RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora	Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii	Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa	Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha	Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana	Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	297
Acc36I	ACCTGC	1 cut(s)	1587
Acc65I	GGTACC	1 cut(s)	179
AccB1I	GGYRCC	1 cut(s)	179
AccB7I	CCANNNNNTGG	2 cut(s)	587, 1573
AccII	CGCG	2 cut(s)	1527, 1655
AciI	CCGC	5 cut(s)	183, 387, 710, 1284, 1653
AclI	AACGTT	1 cut(s)	1233
AclWI	GGATC	6 cut(s)	373, 386, 503, 609, 727, 1520
AcoI	YGGCCR	1 cut(s)	78
AcsI	RAATTY	4 cut(s)	760, 1135, 1201, 1614
AfaI	GTAC	5 cut(s)	181, 515, 609, 822, 968
AfiI	CCNNNNNNNGG	5 cut(s)	587, 953, 1449, 1573, 1640
AflIII	ACRYGT	1 cut(s)	1525
AgsI	TTSAA	9 cut(s)	59, 71, 217, 335, 458, 755, 1016, 1243, 1613
AjnI	CCWGG	2 cut(s)	1168, 1351
AjuI	GAANNNNNNNTTGG	2 cut(s)	455, 487
AleI	CACNNNNGTG	1 cut(s)	509
AluBI	AGCT	4 cut(s)	105, 425, 665, 1034
AluI	AGCT	4 cut(s)	105, 425, 665, 1034
Alw21I	GWGCWC	1 cut(s)	231
Alw26I	GTCTC	3 cut(s)	317, 649, 681
AlwI	GGATC	6 cut(s)	373, 386, 503, 609, 727, 1520
Ama87I	CYCGRG	1 cut(s)	1072
AoxI	GGCC	5 cut(s)	78, 895, 1079, 1225, 1440
ApeKI	GCWGC	2 cut(s)	200, 662
ApoI	RAATTY	4 cut(s)	760, 1135, 1201, 1614
Asp700I	GAANNNNTTC	1 cut(s)	1295
Asp718I	GGTACC	1 cut(s)	179
AspS9I	GGNCC	5 cut(s)	896, 1079, 1172, 1226, 1314
AsuC2I	CCSGG	1 cut(s)	899
AsuHPI	GGTGA	3 cut(s)	16, 1360, 1623
AvaI	CYCGRG	1 cut(s)	1072
AvaII	GGWCC	2 cut(s)	1172, 1314
BalI	TGGCCA	1 cut(s)	80
BamHI	GGATCC	1 cut(s)	378
BanI	GGYRCC	1 cut(s)	179
BanII	GRGCYC	1 cut(s)	632
BauI	CACGAG	1 cut(s)	391
BbsI	GAAGAC	2 cut(s)	507, 1601
Bbv12I	GWGCWC	1 cut(s)	231
BbvI	GCAGC	2 cut(s)	212, 674
BccI	CCATC	5 cut(s)	60, 278, 311, 347, 951
BciT130I	CCWGG	2 cut(s)	1170, 1353
BcnI	CCSGG	1 cut(s)	899
BcoDI	GTCTC	3 cut(s)	317, 649, 681
BfaI	CTAG	3 cut(s)	618, 692, 1113
BfmI	CTRYAG	1 cut(s)	1099
BfuAI	ACCTGC	1 cut(s)	1587
BisI	GCNGC	4 cut(s)	201, 388, 663, 1285
BlsI	GCNGC	4 cut(s)	202, 389, 664, 1286
Bme1390I	CCNGG	3 cut(s)	899, 1170, 1353
Bme18I	GGWCC	2 cut(s)	1172, 1314
BmeT110I	CYCGRG	1 cut(s)	1072
BmgT120I	GGNCC	5 cut(s)	896, 1079, 1172, 1226, 1314
BmiI	GGNNCC	2 cut(s)	181, 380
BmrFI	CCNGG	3 cut(s)	899, 1170, 1353
BmrI	ACTGGG	2 cut(s)	620, 923
BmsI	GCATC	5 cut(s)	266, 1309, 1399, 1457, 1507
BmuI	ACTGGG	2 cut(s)	620, 923
BpiI	GAAGAC	2 cut(s)	507, 1601
BpmI	CTGGAG	1 cut(s)	287
BpuEI	CTTGAG	1 cut(s)	264
BpuMI	CCSGG	1 cut(s)	899
Bsa29I	ATCGAT	1 cut(s)	1515
BsaAI	YACGTR	2 cut(s)	607, 820
BsaBI	GATNNNNATC	1 cut(s)	737
BsaI	GGTCTC	1 cut(s)	681
BsaJI	CCNNGG	3 cut(s)	382, 1459, 1634
Bsc4I	CCNNNNNNNGG	5 cut(s)	587, 953, 1449, 1573, 1640
Bse118I	RCCGGY	1 cut(s)	1558
Bse1I	ACTGG	5 cut(s)	329, 615, 929, 1010, 1443
Bse3DI	GCAATG	1 cut(s)	1067
Bse8I	GATNNNNATC	1 cut(s)	737
BseBI	CCWGG	2 cut(s)	1170, 1353
BseCI	ATCGAT	1 cut(s)	1515
BseDI	CCNNGG	3 cut(s)	382, 1459, 1634
BseGI	GGATG	3 cut(s)	943, 1472, 1649
BseJI	GATNNNNATC	1 cut(s)	737
BseLI	CCNNNNNNNGG	5 cut(s)	587, 953, 1449, 1573, 1640
BseMI	GCAATG	1 cut(s)	1067
BseMII	CTCAG	1 cut(s)	81
BseNI	ACTGG	5 cut(s)	329, 615, 929, 1010, 1443
BseRI	GAGGAG	2 cut(s)	789, 858
BseXI	GCAGC	2 cut(s)	212, 674
BsgI	GTGCAG	1 cut(s)	870
Bsh1236I	CGCG	2 cut(s)	1527, 1655
BshFI	GGCC	5 cut(s)	80, 897, 1081, 1227, 1442
BshNI	GGYRCC	1 cut(s)	179
BshVI	ATCGAT	1 cut(s)	1515
BsiHKAI	GWGCWC	1 cut(s)	231
BsiHKCI	CYCGRG	1 cut(s)	1072
BsiSI	CCGG	2 cut(s)	899, 1559
BsiWI	CGTACG	1 cut(s)	820
BslFI	GGGAC	2 cut(s)	868, 877
BslI	CCNNNNNNNGG	5 cut(s)	587, 953, 1449, 1573, 1640
BsmAI	GTCTC	3 cut(s)	317, 649, 681
BsmFI	GGGAC	2 cut(s)	868, 877
BsmI	GAATGC	1 cut(s)	527
BsnI	GGCC	5 cut(s)	80, 897, 1081, 1227, 1442
Bso31I	GGTCTC	1 cut(s)	681
BsoBI	CYCGRG	1 cut(s)	1072
Bsp1286I	GDGCHC	2 cut(s)	231, 632
Bsp1407I	TGTACA	1 cut(s)	513
Bsp143I	GATC	7 cut(s)	133, 378, 495, 601, 732, 1512, 1628
BspACI	CCGC	5 cut(s)	183, 387, 710, 1284, 1653
BspANI	GGCC	5 cut(s)	80, 897, 1081, 1227, 1442
BspCNI	CTCAG	1 cut(s)	82
BspDI	ATCGAT	1 cut(s)	1515
BspFNI	CGCG	2 cut(s)	1527, 1655
BspHI	TCATGA	1 cut(s)	1429
BspLI	GGNNCC	2 cut(s)	181, 380
BspMI	ACCTGC	1 cut(s)	1587
BspPI	GGATC	6 cut(s)	373, 386, 503, 609, 727, 1520
BspT107I	GGYRCC	1 cut(s)	179
BspTNI	GGTCTC	1 cut(s)	681
BsrDI	GCAATG	1 cut(s)	1067
BsrFI	RCCGGY	1 cut(s)	1558
BsrGI	TGTACA	1 cut(s)	513
BsrI	ACTGG	5 cut(s)	329, 615, 929, 1010, 1443
BssAI	RCCGGY	1 cut(s)	1558
BssECI	CCNNGG	3 cut(s)	382, 1459, 1634
BssMI	GATC	7 cut(s)	133, 378, 495, 601, 732, 1512, 1628
BssSI	CACGAG	1 cut(s)	391
BssT1I	CCWWGG	1 cut(s)	1634
Bst2BI	CACGAG	1 cut(s)	391
Bst2UI	CCWGG	2 cut(s)	1170, 1353
Bst4CI	ACNGT	7 cut(s)	367, 1090, 1103, 1222, 1296, 1463, 1493
Bst6I	CTCTTC	1 cut(s)	748
BstAUI	TGTACA	1 cut(s)	513
BstBAI	YACGTR	2 cut(s)	607, 820
BstC8I	GCNNGC	2 cut(s)	409, 1564
BstDEI	CTNAG	2 cut(s)	90, 884
BstDSI	CCRYGG	1 cut(s)	1459
BstF5I	GGATG	3 cut(s)	943, 1472, 1649
BstFNI	CGCG	2 cut(s)	1527, 1655
BstKTI	GATC	7 cut(s)	136, 381, 498, 604, 735, 1515, 1631
BstMAI	GTCTC	3 cut(s)	317, 649, 681
BstMBI	GATC	7 cut(s)	133, 378, 495, 601, 732, 1512, 1628
BstMWI	GCNNNNNNNGC	2 cut(s)	393, 716
BstNI	CCWGG	2 cut(s)	1170, 1353
BstNSI	RCATGY	1 cut(s)	662
BstSCI	CCNGG	3 cut(s)	897, 1168, 1351
BstSFI	CTRYAG	1 cut(s)	1099
BstSNI	TACGTA	1 cut(s)	820
BstUI	CGCG	2 cut(s)	1527, 1655
BstV1I	GCAGC	2 cut(s)	212, 674
BstV2I	GAAGAC	2 cut(s)	507, 1601
BstX2I	RGATCY	2 cut(s)	378, 732
BstYI	RGATCY	2 cut(s)	378, 732
Bsu15I	ATCGAT	1 cut(s)	1515
BsuRI	GGCC	5 cut(s)	80, 897, 1081, 1227, 1442
BsuTUI	ATCGAT	1 cut(s)	1515
BtgI	CCRYGG	1 cut(s)	1459
BtgZI	GCGATG	1 cut(s)	900
BtsCI	GGATG	3 cut(s)	943, 1472, 1649
BtsIMutI	CAGTG	3 cut(s)	129, 1306, 1436
BveI	ACCTGC	1 cut(s)	1587
Cac8I	GCNNGC	2 cut(s)	409, 1564
CciI	TCATGA	1 cut(s)	1429
Cfr10I	RCCGGY	1 cut(s)	1558
Cfr13I	GGNCC	5 cut(s)	896, 1079, 1172, 1226, 1314
ClaI	ATCGAT	1 cut(s)	1515
Csp6I	GTAC	5 cut(s)	180, 514, 608, 821, 967
CspCI	CAANNNNNGTGG	4 cut(s)	99, 134, 1249, 1284
CviAII	CATG	8 cut(s)	95, 343, 466, 659, 716, 1184, 1288, 1430
CviQI	GTAC	5 cut(s)	180, 514, 608, 821, 967
DdeI	CTNAG	2 cut(s)	90, 884
DpnI	GATC	7 cut(s)	135, 380, 497, 603, 734, 1514, 1630
DpnII	GATC	7 cut(s)	133, 378, 495, 601, 732, 1512, 1628
DraI	TTTAAA	1 cut(s)	1327
EaeI	YGGCCR	1 cut(s)	78
Eam1104I	CTCTTC	1 cut(s)	748
EarI	CTCTTC	1 cut(s)	748
Eco105I	TACGTA	1 cut(s)	820
Eco130I	CCWWGG	1 cut(s)	1634
Eco24I	GRGCYC	1 cut(s)	632
Eco31I	GGTCTC	1 cut(s)	681
Eco47I	GGWCC	2 cut(s)	1172, 1314
Eco88I	CYCGRG	1 cut(s)	1072
EcoO109I	RGGNCCY	1 cut(s)	1172
EcoRII	CCWGG	2 cut(s)	1168, 1351
EcoT14I	CCWWGG	1 cut(s)	1634
EcoT22I	ATGCAT	4 cut(s)	529, 1324, 1472, 1522
EcoT38I	GRGCYC	1 cut(s)	632
ErhI	CCWWGG	1 cut(s)	1634
FaeI	CATG	8 cut(s)	98, 346, 469, 662, 719, 1187, 1291, 1433
FaqI	GGGAC	2 cut(s)	868, 877
FatI	CATG	8 cut(s)	94, 342, 465, 658, 715, 1183, 1287, 1429
FauNDI	CATATG	1 cut(s)	487
Fnu4HI	GCNGC	4 cut(s)	201, 388, 663, 1285
FokI	GGATG	3 cut(s)	930, 1479, 1636
FriOI	GRGCYC	1 cut(s)	632
Fsp4HI	GCNGC	4 cut(s)	201, 388, 663, 1285
FspBI	CTAG	3 cut(s)	618, 692, 1113
GluI	GCNGC	4 cut(s)	201, 388, 663, 1285
GsuI	CTGGAG	1 cut(s)	287
HaeIII	GGCC	5 cut(s)	80, 897, 1081, 1227, 1442
HapII	CCGG	2 cut(s)	899, 1559
Hin1II	CATG	8 cut(s)	98, 346, 469, 662, 719, 1187, 1291, 1433
HincII	GTYRAC	1 cut(s)	1489
HindII	GTYRAC	1 cut(s)	1489
HpaII	CCGG	2 cut(s)	899, 1559
HphI	GGTGA	3 cut(s)	16, 1360, 1623
Hpy166II	GTNNAC	3 cut(s)	415, 514, 1489
Hpy188I	TCNGA	5 cut(s)	64, 91, 267, 349, 1587
Hpy188III	TCNNGA	4 cut(s)	274, 281, 450, 1430
Hpy8I	GTNNAC	3 cut(s)	415, 514, 1489
HpyAV	CCTTC	4 cut(s)	96, 369, 1157, 1355
HpyCH4III	ACNGT	7 cut(s)	367, 1090, 1103, 1222, 1296, 1463, 1493
HpyCH4IV	ACGT	5 cut(s)	606, 723, 819, 951, 1233
HpyF10VI	GCNNNNNNNGC	2 cut(s)	393, 716
HpyF3I	CTNAG	2 cut(s)	90, 884
HpySE526I	ACGT	5 cut(s)	606, 723, 819, 951, 1233
Hsp92II	CATG	8 cut(s)	98, 346, 469, 662, 719, 1187, 1291, 1433
KpnI	GGTACC	1 cut(s)	183
Kzo9I	GATC	7 cut(s)	133, 378, 495, 601, 732, 1512, 1628
Lsp1109I	GCAGC	2 cut(s)	212, 674
LweI	GCATC	5 cut(s)	266, 1309, 1399, 1457, 1507
MaeI	CTAG	3 cut(s)	618, 692, 1113
MaeII	ACGT	5 cut(s)	606, 723, 819, 951, 1233
MaeIII	GTNAC	3 cut(s)	1027, 1216, 1552
MalI	GATC	7 cut(s)	135, 380, 497, 603, 734, 1514, 1630
MboI	GATC	7 cut(s)	133, 378, 495, 601, 732, 1512, 1628
MfeI	CAATTG	1 cut(s)	1023
MflI	RGATCY	2 cut(s)	378, 732
MhlI	GDGCHC	2 cut(s)	231, 632
MlsI	TGGCCA	1 cut(s)	80
MluCI	AATT	8 cut(s)	760, 982, 1023, 1051, 1135, 1201, 1243, 1614
MluI	ACGCGT	1 cut(s)	1525
MluNI	TGGCCA	1 cut(s)	80
MlyI	GAGTC	4 cut(s)	271, 786, 859, 911
MmeI	TCCRAC	2 cut(s)	151, 1456
Mox20I	TGGCCA	1 cut(s)	80
Mph1103I	ATGCAT	4 cut(s)	529, 1324, 1472, 1522
MroXI	GAANNNNTTC	1 cut(s)	1295
MscI	TGGCCA	1 cut(s)	80
MseI	TTAA	6 cut(s)	123, 477, 678, 786, 1310, 1326
MslI	CAYNNNNRTG	3 cut(s)	490, 509, 1654
Msp20I	TGGCCA	1 cut(s)	80
MspA1I	CMGCKG	1 cut(s)	665
MspI	CCGG	2 cut(s)	899, 1559
MspR9I	CCNGG	3 cut(s)	899, 1170, 1353
MunI	CAATTG	1 cut(s)	1023
Mva1269I	GAATGC	1 cut(s)	527
MvaI	CCWGG	2 cut(s)	1170, 1353
MvnI	CGCG	2 cut(s)	1527, 1655
MwoI	GCNNNNNNNGC	2 cut(s)	393, 716
NciI	CCSGG	1 cut(s)	899
NdeI	CATATG	1 cut(s)	487
NdeII	GATC	7 cut(s)	133, 378, 495, 601, 732, 1512, 1628
NlaIII	CATG	8 cut(s)	98, 346, 469, 662, 719, 1187, 1291, 1433
NlaIV	GGNNCC	2 cut(s)	181, 380
NmeAIII	GCCGAG	1 cut(s)	363
NmuCI	GTSAC	2 cut(s)	1216, 1552
NsiI	ATGCAT	4 cut(s)	529, 1324, 1472, 1522
NspI	RCATGY	1 cut(s)	662
OliI	CACNNNNGTG	1 cut(s)	509
PagI	TCATGA	1 cut(s)	1429
PctI	GAATGC	1 cut(s)	527
PdmI	GAANNNNTTC	1 cut(s)	1295
PfeI	GAWTC	7 cut(s)	591, 621, 755, 791, 924, 1433, 1535
Pfl23II	CGTACG	1 cut(s)	820
PflMI	CCANNNNNTGG	2 cut(s)	587, 1573
PfoI	TCCNGGA	1 cut(s)	1168
PkrI	GCNGC	4 cut(s)	202, 389, 664, 1286
PleI	GAGTC	4 cut(s)	271, 785, 859, 910
PpsI	GAGTC	4 cut(s)	271, 785, 859, 910
Ppu21I	YACGTR	2 cut(s)	607, 820
PpuMI	RGGWCCY	1 cut(s)	1172
PsiI	TTATAA	1 cut(s)	297
Psp1406I	AACGTT	1 cut(s)	1233
Psp5II	RGGWCCY	1 cut(s)	1172
Psp6I	CCWGG	2 cut(s)	1168, 1351
PspGI	CCWGG	2 cut(s)	1168, 1351
PspLI	CGTACG	1 cut(s)	820
PspN4I	GGNNCC	2 cut(s)	181, 380
PspPI	GGNCC	5 cut(s)	896, 1079, 1172, 1226, 1314
PspPPI	RGGWCCY	1 cut(s)	1172
PsuI	RGATCY	2 cut(s)	378, 732
PvuII	CAGCTG	1 cut(s)	665
RsaI	GTAC	5 cut(s)	181, 515, 609, 822, 968
RsaNI	GTAC	5 cut(s)	180, 514, 608, 821, 967
RseI	CAYNNNNRTG	3 cut(s)	490, 509, 1654
SaqAI	TTAA	6 cut(s)	123, 477, 678, 786, 1310, 1326
SatI	GCNGC	4 cut(s)	201, 388, 663, 1285
Sau3AI	GATC	7 cut(s)	133, 378, 495, 601, 732, 1512, 1628
Sau96I	GGNCC	5 cut(s)	896, 1079, 1172, 1226, 1314
SchI	GAGTC	4 cut(s)	271, 786, 859, 911
ScrFI	CCNGG	3 cut(s)	899, 1170, 1353
SduI	GDGCHC	2 cut(s)	231, 632
SfaNI	GCATC	5 cut(s)	266, 1309, 1399, 1457, 1507
SfcI	CTRYAG	1 cut(s)	1099
SgrAI	CRCCGGYG	1 cut(s)	1558
SinI	GGWCC	2 cut(s)	1172, 1314
SmiMI	CAYNNNNRTG	3 cut(s)	490, 509, 1654
SmlI	CTYRAG	1 cut(s)	279
SmoI	CTYRAG	1 cut(s)	279
SnaBI	TACGTA	1 cut(s)	820
Sse9I	AATT	8 cut(s)	760, 982, 1023, 1051, 1135, 1201, 1243, 1614
SsiI	CCGC	5 cut(s)	183, 387, 710, 1284, 1653
SspMI	CTAG	3 cut(s)	618, 692, 1113
StyD4I	CCNGG	3 cut(s)	897, 1168, 1351
StyI	CCWWGG	1 cut(s)	1634
TaaI	ACNGT	7 cut(s)	367, 1090, 1103, 1222, 1296, 1463, 1493
TaiI	ACGT	5 cut(s)	609, 726, 822, 954, 1236
TaqI	TCGA	4 cut(s)	498, 1139, 1416, 1515
TaqII	GACCGA	2 cut(s)	801, 1331
TasI	AATT	8 cut(s)	760, 982, 1023, 1051, 1135, 1201, 1243, 1614
TatI	WGTACW	1 cut(s)	513
TauI	GCSGC	2 cut(s)	390, 1287
TfiI	GAWTC	7 cut(s)	591, 621, 755, 791, 924, 1433, 1535
Tru1I	TTAA	6 cut(s)	123, 477, 678, 786, 1310, 1326
Tru9I	TTAA	6 cut(s)	123, 477, 678, 786, 1310, 1326
TscAI	CASTG	3 cut(s)	136, 1306, 1443
TseFI	GTSAC	2 cut(s)	1216, 1552
TseI	GCWGC	2 cut(s)	200, 662
Tsp45I	GTSAC	2 cut(s)	1216, 1552
TspDTI	ATGAA	6 cut(s)	423, 684, 704, 976, 1194, 1304
TspRI	CASTG	3 cut(s)	136, 1306, 1443
Van91I	CCANNNNNTGG	2 cut(s)	587, 1573
VpaK11BI	GGWCC	2 cut(s)	1172, 1314
XapI	RAATTY	4 cut(s)	760, 1135, 1201, 1614
XceI	RCATGY	1 cut(s)	662
XcmI	CCANNNNNNNNNTGG	1 cut(s)	950
XmnI	GAANNNNTTC	1 cut(s)	1295
XspI	CTAG	3 cut(s)	618, 692, 1113
Zsp2I	ATGCAT	4 cut(s)	529, 1324, 1472, 1522

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.