pycom03g07320

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000533
Physical Location & Seq
Forward (+)
5852264 .. 5854596
2333 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g07320.3

Sequence Viewer

Length: 1821 bp
ATGGAGAAATCAACAACGGCTGCTATATTATTGCTCTTGTACTTTTTTGGGTTCTGCCCTCAACCAGGGGTTCCCTCGTTCGCCAATCCATCTGATGACCTTGAATATGACTACATTGTTGTGGGAGGGGGCACAGCAGGGTGCCCGTTGGCAACAACTTTATCTGCAAACTACTCCGTTCTCCTTCTAGAAAGGGGCAATATTCCATCTGCATATCCAAATGTGTTGCGTGCCAATGAGACTCTTGCAAATTTCATGCAAGAAGATGACGGTAAGATACCGGCCCAGAGGTTCACATCAGAAGATGGCGTGGCTAATTTAAGAGGACGGATCCTAGGCGGGTCAAGTATGATCAATATTGGCATATACTCAAGAGCCGACAGTGAATTCTACGAGAAATCAGGAATTAAATTGGACATGAACTTAGTCAATAATTCATATGAATGGGTTGAAAACACTGTGATATTCCGTCCGAATGTAACACGTTGGCAGTCTGTTGTGAAAGATGCGATGTTAGAAGCTGGTGTTCGTCCAGACAATGGACTGACTTTGGATCACATTCTGGGAAGTAAAGTCACGGGTACGCTTTTTGACAATCGTGGAAAAAGACATGGAGCTGTGGAACTGTTAAATAAAGGACATCCAAAAAATCTGAGAGTTGCAATTCATGCCACAGTAGAGAGGATAATTTTCTCTTCCGAAGCATCAGGTATCCAAACACATTTGATATGTTTGTCAGCTAAAGGAATCATATACAGTGATTCTAACGGGAGGTCTCATCGGGCATTGATACGTGGTAAGGGTGAGGTTATATTGAGTGCAGGTGCAATTGGAAGTCCTCAACTTCTACTACTTAGTGGTGTTGGCCCAAAATCGTACCTTTTATCTTGTAAAATCCCAGTTGTTCACCCACAACCTTACGTTGGGCAGTTTATGCGTGATAATCCTCGTAATTACATTACCATTTTGCCCCCATTTCAAGTAGAACCTTCTACTGCACAGGTTGTTGGTATCACAAGTGATTATTACATAGAGACTTTCTCCGGCTTGCCATATTCTACTCAGGCCTTTAGTCTTTTCCCTAGTCCAACTATTCCCATGACCATAAATTCAAGTTTCGGGCAAATTGTTGTCAAATTTCCAGGACCCTTGTCCTACGGTTCTCTTAAACTGCAATCATCCTATGATGTCAAAGTTGCTCCAAATGTCAAATTCAACTACTTTGCACAGGAGGCAGACCTTTCTCGTTGTGTTAGTGCTGTGAGGAAAATGGGTGATTTATTAAAAACAAATTCATTGAAACCGTATAAGGCTCAAGATTTGCCAGGTCTAGAAGGTTTCAACCTTTTTGGACCGCCCTTACCAGTGAACCAGTCAGACGATGCATCCTTTGAAACCTTTTGTCGAGATACAGTAGCCTCATTTTGGCACTACCATGGTGGATGCCTGGTCGGAAAGGTAGTTGATGAAGATTTGCGGGTCATGGGGATCAAGGCATTACGTGTTGTTGATGGATCTGTATTCAATTTATCACCAGGGACCAATCCTCAAGCCACTCTTATGATGTTAGGCAGGTATGCATGTTACATGCATTTAGTTAAGTACACAACCACAAGAAATTACCTGTACATAATACAACTAAGTAAATATTTAACTAAGTCCTTAATTTGCTTATTTATTTATTTATTTGTATGTTGGCGTTCAAATGCTGGAAGAAAGATCAGAGTGACACGTTTCTTCGAGATTATATTCAATTTGTCATACAATGGATTGAAGATTATTCAACAACCTTTAGTGTTATCGTGTTTTTACTTAAGATAA

Protein Analysis

607

Amino Acids

67.03

Weight (kDa)

8.96

Isoelectric Point (pI)

38.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 812
Acc36I ACCTGC 2 cut(s) 812, 1563
AccB1I GGYRCC 1 cut(s) 141
AccB7I CCANNNNNTGG 1 cut(s) 539
AciI CCGC 3 cut(s) 339, 1355, 1477
AclWI GGATC 5 cut(s) 325, 338, 561, 1496, 1522
AcsI RAATTY 6 cut(s) 250, 386, 1108, 1136, 1211, 1291
AfaI GTAC 5 cut(s) 41, 583, 878, 1604, 1628
AfiI CCNNNNNNNGG 4 cut(s) 65, 539, 923, 1425
AflII CTTAAG 1 cut(s) 1813
AflIII ACRYGT 3 cut(s) 482, 1501, 1730
AjnI CCWGG 5 cut(s) 64, 1141, 1324, 1446, 1534
AluBI AGCT 3 cut(s) 521, 617, 740
AluI AGCT 3 cut(s) 521, 617, 740
Alw26I GTCTC 3 cut(s) 233, 780, 1028
AlwI GGATC 5 cut(s) 325, 338, 561, 1496, 1522
AoxI GGCC 3 cut(s) 282, 865, 1065
ApeKI GCWGC 1 cut(s) 20
ApoI RAATTY 6 cut(s) 250, 386, 1108, 1136, 1211, 1291
AspA2I CCTAGG 1 cut(s) 334
AspS9I GGNCC 5 cut(s) 283, 866, 1145, 1352, 1539
AsuHPI GGTGA 4 cut(s) 815, 899, 1286, 1524
AvaII GGWCC 3 cut(s) 1145, 1352, 1539
AvrII CCTAGG 1 cut(s) 334
BaeGI GKGCMC 2 cut(s) 134, 146
BamHI GGATCC 1 cut(s) 330
BanI GGYRCC 1 cut(s) 141
BbvI GCAGC 1 cut(s) 7
BccI CCATC 4 cut(s) 97, 214, 299, 1505
BceAI ACGGC 1 cut(s) 33
BciT130I CCWGG 5 cut(s) 66, 1143, 1326, 1448, 1536
BciVI GTATCC 1 cut(s) 722
BclI TGATCA 1 cut(s) 351
BcoDI GTCTC 3 cut(s) 233, 780, 1028
BfaI CTAG 4 cut(s) 188, 335, 1083, 1331
BfrI CTTAAG 1 cut(s) 1813
BfuAI ACCTGC 2 cut(s) 812, 1563
BfuI GTATCC 1 cut(s) 722
BisI GCNGC 1 cut(s) 21
BlnI CCTAGG 1 cut(s) 334
BlsI GCNGC 1 cut(s) 22
Bme1390I CCNGG 5 cut(s) 66, 1143, 1326, 1448, 1536
Bme18I GGWCC 3 cut(s) 1145, 1352, 1539
BmgT120I GGNCC 5 cut(s) 283, 866, 1145, 1352, 1539
BmiI GGNNCC 5 cut(s) 72, 143, 332, 1147, 1540
BmrFI CCNGG 5 cut(s) 66, 1143, 1326, 1448, 1536
BmrI ACTGGG 1 cut(s) 893
BmsI GCATC 5 cut(s) 496, 713, 1372, 1394, 1433
BmuI ACTGGG 1 cut(s) 893
BoxI GACNNNNGTC 1 cut(s) 1150
BplI GAGNNNNNCTC 2 cut(s) 1025, 1057
BpuEI CTTGAG 3 cut(s) 355, 1299, 1533
BsaAI YACGTR 2 cut(s) 794, 1502
BsaI GGTCTC 1 cut(s) 780
BsaJI CCNNGG 4 cut(s) 65, 334, 1435, 1535
Bsc4I CCNNNNNNNGG 4 cut(s) 65, 539, 923, 1425
Bse118I RCCGGY 1 cut(s) 280
Bse1I ACTGG 3 cut(s) 899, 1364, 1372
BseBI CCWGG 5 cut(s) 66, 1143, 1326, 1448, 1536
BseDI CCNNGG 4 cut(s) 65, 334, 1435, 1535
BseGI GGATG 4 cut(s) 640, 1178, 1385, 1448
BseLI CCNNNNNNNGG 4 cut(s) 65, 539, 923, 1425
BseMII CTCAG 2 cut(s) 644, 1076
BseNI ACTGG 3 cut(s) 899, 1364, 1372
BseSI GKGCMC 2 cut(s) 134, 146
BseXI GCAGC 1 cut(s) 7
BsgI GTGCAG 2 cut(s) 840, 981
BshFI GGCC 3 cut(s) 284, 867, 1067
BshNI GGYRCC 1 cut(s) 141
BsiSI CCGG 2 cut(s) 281, 1044
BslFI GGGAC 1 cut(s) 1552
BslI CCNNNNNNNGG 4 cut(s) 65, 539, 923, 1425
BsmAI GTCTC 3 cut(s) 233, 780, 1028
BsmFI GGGAC 1 cut(s) 1552
BsnI GGCC 3 cut(s) 284, 867, 1067
Bso31I GGTCTC 1 cut(s) 780
Bsp1286I GDGCHC 2 cut(s) 134, 146
Bsp1407I TGTACA 1 cut(s) 1626
Bsp143I GATC 6 cut(s) 330, 351, 553, 1488, 1514, 1719
Bsp19I CCATGG 1 cut(s) 1435
BspACI CCGC 3 cut(s) 339, 1355, 1477
BspANI GGCC 3 cut(s) 284, 867, 1067
BspCNI CTCAG 2 cut(s) 645, 1075
BspLI GGNNCC 5 cut(s) 72, 143, 332, 1147, 1540
BspMI ACCTGC 2 cut(s) 812, 1563
BspPI GGATC 5 cut(s) 325, 338, 561, 1496, 1522
BspT107I GGYRCC 1 cut(s) 141
BspTI CTTAAG 1 cut(s) 1813
BspTNI GGTCTC 1 cut(s) 780
BsrFI RCCGGY 1 cut(s) 280
BsrGI TGTACA 1 cut(s) 1626
BsrI ACTGG 3 cut(s) 899, 1364, 1372
BssAI RCCGGY 1 cut(s) 280
BssECI CCNNGG 4 cut(s) 65, 334, 1435, 1535
BssMI GATC 6 cut(s) 330, 351, 553, 1488, 1514, 1719
BssT1I CCWWGG 2 cut(s) 334, 1435
Bst2UI CCWGG 5 cut(s) 66, 1143, 1326, 1448, 1536
Bst4CI ACNGT 9 cut(s) 272, 383, 460, 627, 676, 758, 1160, 1305, 1414
Bst6I CTCTTC 1 cut(s) 700
BstAFI CTTAAG 1 cut(s) 1813
BstAPI GCANNNNNTGC 2 cut(s) 668, 934
BstAUI TGTACA 1 cut(s) 1626
BstBAI YACGTR 2 cut(s) 794, 1502
BstC8I GCNNGC 2 cut(s) 231, 1049
BstDEI CTNAG 6 cut(s) 424, 653, 854, 1062, 1640, 1656
BstDSI CCRYGG 1 cut(s) 1435
BstENI CCTNNNNNAGG 1 cut(s) 63
BstF5I GGATG 4 cut(s) 640, 1178, 1385, 1448
BstKTI GATC 6 cut(s) 333, 354, 556, 1491, 1517, 1722
BstMAI GTCTC 3 cut(s) 233, 780, 1028
BstMBI GATC 6 cut(s) 330, 351, 553, 1488, 1514, 1719
BstMWI GCNNNNNNNGC 3 cut(s) 668, 934, 1232
BstNI CCWGG 5 cut(s) 66, 1143, 1326, 1448, 1536
BstNSI RCATGY 2 cut(s) 1584, 1591
BstPAI GACNNNNGTC 1 cut(s) 1150
BstSCI CCNGG 5 cut(s) 64, 1141, 1324, 1446, 1534
BstSLI GKGCMC 2 cut(s) 134, 146
BstV1I GCAGC 1 cut(s) 7
BstX2I RGATCY 2 cut(s) 330, 1514
BstYI RGATCY 2 cut(s) 330, 1514
BsuI GTATCC 1 cut(s) 722
BsuRI GGCC 3 cut(s) 284, 867, 1067
BtgI CCRYGG 1 cut(s) 1435
BtgZI GCGATG 1 cut(s) 524
BtsCI GGATG 4 cut(s) 640, 1178, 1385, 1448
BtsIMutI CAGTG 4 cut(s) 388, 456, 763, 1371
BveI ACCTGC 2 cut(s) 812, 1563
Cac8I GCNNGC 2 cut(s) 231, 1049
Cfr10I RCCGGY 1 cut(s) 280
Cfr13I GGNCC 5 cut(s) 283, 866, 1145, 1352, 1539
Csp6I GTAC 5 cut(s) 40, 582, 877, 1603, 1627
CviAII CATG 9 cut(s) 256, 418, 611, 668, 1099, 1436, 1483, 1581, 1588
CviQI GTAC 5 cut(s) 40, 582, 877, 1603, 1627
DdeI CTNAG 6 cut(s) 424, 653, 854, 1062, 1640, 1656
DpnI GATC 6 cut(s) 332, 353, 555, 1490, 1516, 1721
DpnII GATC 6 cut(s) 330, 351, 553, 1488, 1514, 1719
Eam1104I CTCTTC 1 cut(s) 700
EarI CTCTTC 1 cut(s) 700
Eco130I CCWWGG 2 cut(s) 334, 1435
Eco147I AGGCCT 1 cut(s) 1067
Eco31I GGTCTC 1 cut(s) 780
Eco47I GGWCC 3 cut(s) 1145, 1352, 1539
EcoNI CCTNNNNNAGG 1 cut(s) 63
EcoO109I RGGNCCY 1 cut(s) 1145
EcoRI GAATTC 1 cut(s) 386
EcoRII CCWGG 5 cut(s) 64, 1141, 1324, 1446, 1534
EcoT14I CCWWGG 2 cut(s) 334, 1435
EcoT22I ATGCAT 3 cut(s) 1387, 1582, 1593
ErhI CCWWGG 2 cut(s) 334, 1435
FaeI CATG 9 cut(s) 259, 421, 614, 671, 1102, 1439, 1486, 1584, 1591
FalI AAGNNNNNCTT 2 cut(s) 1542, 1574
FaqI GGGAC 1 cut(s) 1552
FatI CATG 9 cut(s) 255, 417, 610, 667, 1098, 1435, 1482, 1580, 1587
FauI CCCGC 2 cut(s) 332, 1470
FauNDI CATATG 1 cut(s) 439
FbaI TGATCA 1 cut(s) 351
Fnu4HI GCNGC 1 cut(s) 21
FokI GGATG 4 cut(s) 627, 1165, 1372, 1455
Fsp4HI GCNGC 1 cut(s) 21
FspBI CTAG 4 cut(s) 188, 335, 1083, 1331
GluI GCNGC 1 cut(s) 21
HaeIII GGCC 3 cut(s) 284, 867, 1067
HapII CCGG 2 cut(s) 281, 1044
Hin1II CATG 9 cut(s) 259, 421, 614, 671, 1102, 1439, 1486, 1584, 1591
HinfI GANTC 3 cut(s) 241, 747, 761
HpaII CCGG 2 cut(s) 281, 1044
HphI GGTGA 4 cut(s) 815, 899, 1286, 1524
Hpy166II GTNNAC 4 cut(s) 294, 907, 1369, 1605
Hpy188I TCNGA 8 cut(s) 94, 301, 474, 654, 700, 1378, 1454, 1724
Hpy188III TCNNGA 8 cut(s) 188, 372, 402, 533, 1316, 1331, 1406, 1741
Hpy8I GTNNAC 4 cut(s) 294, 907, 1369, 1605
HpyAV CCTTC 3 cut(s) 194, 999, 1328
HpyCH4III ACNGT 9 cut(s) 272, 383, 460, 627, 676, 758, 1160, 1305, 1414
HpyCH4IV ACGT 5 cut(s) 484, 793, 921, 1501, 1732
HpyF10VI GCNNNNNNNGC 3 cut(s) 668, 934, 1232
HpyF3I CTNAG 6 cut(s) 424, 653, 854, 1062, 1640, 1656
HpySE526I ACGT 5 cut(s) 484, 793, 921, 1501, 1732
Hsp92II CATG 9 cut(s) 259, 421, 614, 671, 1102, 1439, 1486, 1584, 1591
Ksp22I TGATCA 1 cut(s) 351
Kzo9I GATC 6 cut(s) 330, 351, 553, 1488, 1514, 1719
LmnI GCTCC 2 cut(s) 614, 1204
Lsp1109I GCAGC 1 cut(s) 7
LweI GCATC 5 cut(s) 496, 713, 1372, 1394, 1433
MaeI CTAG 4 cut(s) 188, 335, 1083, 1331
MaeII ACGT 5 cut(s) 484, 793, 921, 1501, 1732
MaeIII GTNAC 4 cut(s) 478, 574, 1583, 1726
MalI GATC 6 cut(s) 332, 353, 555, 1490, 1516, 1721
MboI GATC 6 cut(s) 330, 351, 553, 1488, 1514, 1719
MboII GAAGA 7 cut(s) 275, 314, 687, 1481, 1725, 1729, 1786
MfeI CAATTG 1 cut(s) 828
MflI RGATCY 2 cut(s) 330, 1514
MhlI GDGCHC 2 cut(s) 134, 146
MlyI GAGTC 1 cut(s) 235
MmeI TCCRAC 2 cut(s) 1112, 1432
Mph1103I ATGCAT 3 cut(s) 1387, 1582, 1593
MseI TTAA 9 cut(s) 320, 408, 629, 1167, 1283, 1599, 1652, 1664, 1814
MslI CAYNNNNRTG 4 cut(s) 119, 442, 1434, 1559
MspCI CTTAAG 1 cut(s) 1813
MspI CCGG 2 cut(s) 281, 1044
MspR9I CCNGG 5 cut(s) 66, 1143, 1326, 1448, 1536
MunI CAATTG 1 cut(s) 828
MvaI CCWGG 5 cut(s) 66, 1143, 1326, 1448, 1536
MwoI GCNNNNNNNGC 3 cut(s) 668, 934, 1232
NcoI CCATGG 1 cut(s) 1435
NdeI CATATG 1 cut(s) 439
NdeII GATC 6 cut(s) 330, 351, 553, 1488, 1514, 1719
NlaIII CATG 9 cut(s) 259, 421, 614, 671, 1102, 1439, 1486, 1584, 1591
NlaIV GGNNCC 5 cut(s) 72, 143, 332, 1147, 1540
NmuCI GTSAC 2 cut(s) 574, 1726
NsiI ATGCAT 3 cut(s) 1387, 1582, 1593
NspI RCATGY 2 cut(s) 1584, 1591
PaqCI CACCTGC 1 cut(s) 812
PceI AGGCCT 1 cut(s) 1067
PfeI GAWTC 2 cut(s) 747, 761
PflMI CCANNNNNTGG 1 cut(s) 539
PfoI TCCNGGA 1 cut(s) 1141
PkrI GCNGC 1 cut(s) 22
PleI GAGTC 1 cut(s) 235
PpsI GAGTC 1 cut(s) 235
Ppu21I YACGTR 2 cut(s) 794, 1502
PpuMI RGGWCCY 1 cut(s) 1145
PshAI GACNNNNGTC 1 cut(s) 1150
Psp5II RGGWCCY 1 cut(s) 1145
Psp6I CCWGG 5 cut(s) 64, 1141, 1324, 1446, 1534
PspGI CCWGG 5 cut(s) 64, 1141, 1324, 1446, 1534
PspN4I GGNNCC 5 cut(s) 72, 143, 332, 1147, 1540
PspPI GGNCC 5 cut(s) 283, 866, 1145, 1352, 1539
PspPPI RGGWCCY 1 cut(s) 1145
PsuI RGATCY 2 cut(s) 330, 1514
RsaI GTAC 5 cut(s) 41, 583, 878, 1604, 1628
RsaNI GTAC 5 cut(s) 40, 582, 877, 1603, 1627
RseI CAYNNNNRTG 4 cut(s) 119, 442, 1434, 1559
SaqAI TTAA 9 cut(s) 320, 408, 629, 1167, 1283, 1599, 1652, 1664, 1814
SatI GCNGC 1 cut(s) 21
Sau3AI GATC 6 cut(s) 330, 351, 553, 1488, 1514, 1719
Sau96I GGNCC 5 cut(s) 283, 866, 1145, 1352, 1539
SchI GAGTC 1 cut(s) 235
ScrFI CCNGG 5 cut(s) 66, 1143, 1326, 1448, 1536
SduI GDGCHC 2 cut(s) 134, 146
SfaNI GCATC 5 cut(s) 496, 713, 1372, 1394, 1433
SinI GGWCC 3 cut(s) 1145, 1352, 1539
SmiMI CAYNNNNRTG 4 cut(s) 119, 442, 1434, 1559
SmlI CTYRAG 4 cut(s) 370, 1314, 1548, 1813
SmoI CTYRAG 4 cut(s) 370, 1314, 1548, 1813
SseBI AGGCCT 1 cut(s) 1067
SsiI CCGC 3 cut(s) 339, 1355, 1477
SspI AATATT 3 cut(s) 202, 358, 1649
SspMI CTAG 4 cut(s) 188, 335, 1083, 1331
StuI AGGCCT 1 cut(s) 1067
StyD4I CCNGG 5 cut(s) 64, 1141, 1324, 1446, 1534
StyI CCWWGG 2 cut(s) 334, 1435
TaaI ACNGT 9 cut(s) 272, 383, 460, 627, 676, 758, 1160, 1305, 1414
TaiI ACGT 5 cut(s) 487, 796, 924, 1504, 1735
TaqI TCGA 2 cut(s) 1405, 1740
TatI WGTACW 3 cut(s) 39, 1602, 1626
TfiI GAWTC 2 cut(s) 747, 761
Tru1I TTAA 9 cut(s) 320, 408, 629, 1167, 1283, 1599, 1652, 1664, 1814
Tru9I TTAA 9 cut(s) 320, 408, 629, 1167, 1283, 1599, 1652, 1664, 1814
TscAI CASTG 4 cut(s) 388, 463, 763, 1371
TseFI GTSAC 2 cut(s) 574, 1726
TseI GCWGC 1 cut(s) 20
Tsp45I GTSAC 2 cut(s) 574, 1726
TspDTI ATGAA 7 cut(s) 244, 426, 434, 456, 656, 1284, 1482
TspGWI ACGGA 3 cut(s) 166, 343, 458
TspRI CASTG 4 cut(s) 388, 463, 763, 1371
Van91I CCANNNNNTGG 1 cut(s) 539
Vha464I CTTAAG 1 cut(s) 1813
VpaK11BI GGWCC 3 cut(s) 1145, 1352, 1539
XagI CCTNNNNNAGG 1 cut(s) 63
XapI RAATTY 6 cut(s) 250, 386, 1108, 1136, 1211, 1291
XbaI TCTAGA 2 cut(s) 187, 1330
XceI RCATGY 2 cut(s) 1584, 1591
XmaJI CCTAGG 1 cut(s) 334
XspI CTAG 4 cut(s) 188, 335, 1083, 1331
Zsp2I ATGCAT 3 cut(s) 1387, 1582, 1593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.