RchiOBHm_Chr4g0403711

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000533
Physical Location & Seq
Reverse (-)
23171821 .. 23174701
2881 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37541

Sequence Viewer

Length: 1665 bp
ATGGAGAAATCGACAATAGGTGCTAGACTACTGCTCTTGAACCTTTTTGTACTTGGCTTTCTTGGTCAATCAACAGTTCATTCATTGGCCAATTCATCTGCACCTGATCATGATTTTAGCTACATGAAGATTGTGAAGGATGCCACTGATCTACCGTTGGAAGATAAATATGACTATATTGTAGTTGGGGGAGGAACAGCAGGTTGTCCATTAGCAGCAACATTATCAGCAAGCTACTCGGTGCTTGTTCTTGAAAGAGGTAGTGCTCCTACTGCACATCCAAGTGTCTTGCGAGAAGAAGGGTTTCTGAGTAATCTCATGCAAGAAGATGATGGTAAGATCACACCAGCTCAAAAGTTTAAGTCTGAAGACGGTGTTGCCATTGTCAGAGGAAGGGTTCTTGGTGGCTCAAGCATGATCAATGCTGGTTTCTACTCCAAAGCTGACAGGGAATTCTTTGTTAAATCTGGAATTAAATGGGACATGGATTTGGTTGATAAGGCATATCACTGGGTTGAAAGCACTGTCGTCTTTCGTCCAAATCTGTCACGTTCAAATTGGCAATCTATTGTGAAAGAAGCATTGTTGGAGGCTGGTGTTGGTCCAAACAATGGATTCAGTTTGGATCACATTAAGGGAACTAAAGTTGGCGGTTCTACTTTTGATACACATGGAAGGAGATACGGAGCTGTTGAATTGCTTAATAGAGGAAACCTAAAGAACTTGCGAGTGGGAATTCATGCCACAGTCGAGAAAATCATCTTCTCTTCCAAAGAATCAGGTGTTTCGGCTCGAGGGGTGGTATATATTGATTCTAATGGGAGGTCTCATCGGGCATTTGTTCGTGGTAAGGGAGAGGTTATTCTGAGTGCAGGGGCAATTGGGAGTCCTCAGCTTCTACTTCTTAGCGGTGTTGGTCCTCAGTCCTATCTATCATCTTTGAAAATCCCAGTTGTTCACCCCCAACCTCACACTGGAAACTTTATGTATGACAATCCTCGTAATTTCATTAACATTTTGTCCCCGTTTCCACTACAGCCCTCAACTGTAAAGATTGTCGGCATTACCGACCATTTCTACATAGAGACCCTCTCTAGCATGCCCATTTCTACTACACCCTACAGTGTTTTTCCTGATCCGGCTAAACTTACAAAGATAAATTCAAGTTTAGGACTCATTGCCATCAAATTAGTAGGACCTTTGTCTTATGGTACTTTGAAGCTTCAATCATCATCTGATGTGAGAGTTGGTCCAAATGTCCGCTTCAACTACTTTGCACATCCCTTGGACCTTGCTCGTTGTGTTTCTGGCATGAAGAAGATTGGGGAGTTATTAAATACGAAGTCACTGAAAGTATTTAAGGTTCAAGCTTCGTCGAGCACAAAAGCTTACAATTTTTATGGTCCAACTTTACCTATGAACCAGACAGATGATGCATCTTTTGGAGAATTTTGTCGTCATACAGTATCAACAATTTGGCATTTCCACGGCGGATGCCTTGTCGGAAAGGTGGTCGATAGTAGCTTGCGGGTCATGGGAATCAATGCTTTGCGTATTGTTGACGCATCCACATTCAATTTCACACCGGGGACAAATCCTCAGGCCACACTTATGATGTTGGGCAGGTACATTGGCATTAAGATGCTGCAACAAAGATCTTCTTAA

Protein Analysis

554

Amino Acids

60.15

Weight (kDa)

9.41

Isoelectric Point (pI)

30.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_N PF00732 57 - 310 2.6e-21 GMC oxidoreductase
GMC_oxred_C PF05199 400 - 541 5.8e-25 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 191, 1614
AccB7I CCANNNNNTGG 1 cut(s) 611
AciI CCGC 5 cut(s) 651, 909, 1261, 1491, 1528
AclWI GGATC 2 cut(s) 633, 1130
AcoI YGGCCR 1 cut(s) 87
AcsI RAATTY 4 cut(s) 452, 735, 1159, 1448
AcuI CTGAAG 1 cut(s) 387
AfaI GTAC 3 cut(s) 51, 1213, 1628
AfiI CCNNNNNNNGG 2 cut(s) 611, 974
Alw21I GWGCWC 2 cut(s) 268, 1382
Alw26I GTCTC 2 cut(s) 831, 1079
AlwI GGATC 2 cut(s) 633, 1130
Ama87I CYCGRG 1 cut(s) 792
AoxI GGCC 2 cut(s) 87, 1602
ApeKI GCWGC 2 cut(s) 215, 1645
ApoI RAATTY 4 cut(s) 452, 735, 1159, 1448
Asp700I GAANNNNTTC 1 cut(s) 303
AspS9I GGNCC 6 cut(s) 602, 917, 1196, 1250, 1288, 1403
AsuC2I CCSGG 1 cut(s) 1587
AsuHPI GGTGA 1 cut(s) 950
AvaI CYCGRG 1 cut(s) 792
AvaII GGWCC 6 cut(s) 602, 917, 1196, 1250, 1288, 1403
AxyI CCTNAGG 1 cut(s) 1599
BalI TGGCCA 1 cut(s) 89
BbsI GAAGAC 1 cut(s) 375
Bbv12I GWGCWC 2 cut(s) 268, 1382
BbvCI CCTCAGC 1 cut(s) 891
BbvI GCAGC 2 cut(s) 227, 1632
BccI CCATC 2 cut(s) 326, 1190
BceAI ACGGC 1 cut(s) 1504
BcgI CGANNNNNNTGC 2 cut(s) 219, 253
BclI TGATCA 2 cut(s) 106, 417
BcnI CCSGG 1 cut(s) 1587
BcoDI GTCTC 2 cut(s) 831, 1079
BfaI CTAG 2 cut(s) 24, 1095
BfmI CTRYAG 2 cut(s) 1034, 1120
BfuAI ACCTGC 2 cut(s) 191, 1614
BglII AGATCT 1 cut(s) 1655
BisI GCNGC 2 cut(s) 216, 1646
BlsI GCNGC 2 cut(s) 217, 1647
Bme1390I CCNGG 1 cut(s) 1587
Bme18I GGWCC 6 cut(s) 602, 917, 1196, 1250, 1288, 1403
BmeT110I CYCGRG 1 cut(s) 792
BmgT120I GGNCC 6 cut(s) 602, 917, 1196, 1250, 1288, 1403
BmrFI CCNGG 1 cut(s) 1587
BmrI ACTGGG 2 cut(s) 520, 944
BmsI GCATC 6 cut(s) 130, 1423, 1445, 1484, 1574, 1632
BmuI ACTGGG 2 cut(s) 520, 944
BoxI GACNNNNGTC 1 cut(s) 1201
BpiI GAAGAC 1 cut(s) 375
BplI GAGNNNNNCTC 2 cut(s) 1076, 1108
Bpu10I CCTNAGC 1 cut(s) 891
BpuEI CTTGAG 1 cut(s) 394
BpuMI CCSGG 1 cut(s) 1587
BsaI GGTCTC 2 cut(s) 831, 1079
BsaJI CCNNGG 3 cut(s) 1284, 1486, 1586
Bsc4I CCNNNNNNNGG 2 cut(s) 611, 974
Bse1I ACTGG 3 cut(s) 515, 950, 979
Bse21I CCTNAGG 1 cut(s) 1599
Bse3DI GCAATG 1 cut(s) 1176
BseDI CCNNGG 3 cut(s) 1284, 1486, 1586
BseGI GGATG 5 cut(s) 145, 277, 1279, 1499, 1565
BseLI CCNNNNNNNGG 2 cut(s) 611, 974
BseMI GCAATG 1 cut(s) 1176
BseMII CTCAG 5 cut(s) 299, 857, 905, 935, 1613
BseNI ACTGG 3 cut(s) 515, 950, 979
BseXI GCAGC 2 cut(s) 227, 1632
BsgI GTGCAG 3 cut(s) 84, 258, 891
BshFI GGCC 2 cut(s) 89, 1604
BsiHKAI GWGCWC 2 cut(s) 268, 1382
BsiHKCI CYCGRG 1 cut(s) 792
BsiSI CCGG 2 cut(s) 1139, 1586
BslFI GGGAC 3 cut(s) 494, 1006, 1603
BslI CCNNNNNNNGG 2 cut(s) 611, 974
BsmAI GTCTC 2 cut(s) 831, 1079
BsmFI GGGAC 3 cut(s) 494, 1006, 1603
BsnI GGCC 2 cut(s) 89, 1604
Bso31I GGTCTC 2 cut(s) 831, 1079
BsoBI CYCGRG 1 cut(s) 792
Bsp1286I GDGCHC 2 cut(s) 268, 1382
Bsp143I GATC 7 cut(s) 106, 148, 339, 417, 625, 1135, 1655
BspACI CCGC 5 cut(s) 651, 909, 1261, 1491, 1528
BspANI GGCC 2 cut(s) 89, 1604
BspCNI CTCAG 5 cut(s) 300, 858, 904, 934, 1612
BspHI TCATGA 1 cut(s) 109
BspMI ACCTGC 2 cut(s) 191, 1614
BspPI GGATC 2 cut(s) 633, 1130
BspTNI GGTCTC 2 cut(s) 831, 1079
BsrDI GCAATG 1 cut(s) 1176
BsrI ACTGG 3 cut(s) 515, 950, 979
BssECI CCNNGG 3 cut(s) 1284, 1486, 1586
BssMI GATC 7 cut(s) 106, 148, 339, 417, 625, 1135, 1655
BssT1I CCWWGG 1 cut(s) 1284
Bst4CI ACNGT 8 cut(s) 76, 156, 374, 526, 748, 1048, 1124, 1465
Bst6I CTCTTC 1 cut(s) 772
BstC8I GCNNGC 3 cut(s) 232, 1100, 1526
BstDEI CTNAG 6 cut(s) 308, 866, 891, 905, 921, 1599
BstDSI CCRYGG 1 cut(s) 1486
BstF5I GGATG 5 cut(s) 145, 277, 1279, 1499, 1565
BstKTI GATC 7 cut(s) 109, 151, 342, 420, 628, 1138, 1658
BstMAI GTCTC 2 cut(s) 831, 1079
BstMBI GATC 7 cut(s) 106, 148, 339, 417, 625, 1135, 1655
BstMWI GCNNNNNNNGC 1 cut(s) 272
BstNSI RCATGY 1 cut(s) 1102
BstPAI GACNNNNGTC 1 cut(s) 1201
BstSCI CCNGG 1 cut(s) 1585
BstSFI CTRYAG 2 cut(s) 1034, 1120
BstV1I GCAGC 2 cut(s) 227, 1632
BstV2I GAAGAC 1 cut(s) 375
BstX2I RGATCY 1 cut(s) 1655
BstYI RGATCY 1 cut(s) 1655
Bsu36I CCTNAGG 1 cut(s) 1599
BsuRI GGCC 2 cut(s) 89, 1604
BtgI CCRYGG 1 cut(s) 1486
BtsCI GGATG 5 cut(s) 145, 277, 1279, 1499, 1565
BtsIMutI CAGTG 6 cut(s) 144, 508, 522, 972, 1129, 1346
BveI ACCTGC 2 cut(s) 191, 1614
Cac8I GCNNGC 3 cut(s) 232, 1100, 1526
CciI TCATGA 1 cut(s) 109
Cfr13I GGNCC 6 cut(s) 602, 917, 1196, 1250, 1288, 1403
CseI GACGC 1 cut(s) 1571
Csp6I GTAC 3 cut(s) 50, 1212, 1627
CviQI GTAC 3 cut(s) 50, 1212, 1627
DdeI CTNAG 6 cut(s) 308, 866, 891, 905, 921, 1599
DpnI GATC 7 cut(s) 108, 150, 341, 419, 627, 1137, 1657
DpnII GATC 7 cut(s) 106, 148, 339, 417, 625, 1135, 1655
EaeI YGGCCR 1 cut(s) 87
Eam1104I CTCTTC 1 cut(s) 772
EarI CTCTTC 1 cut(s) 772
EciI GGCGGA 1 cut(s) 1506
Eco130I CCWWGG 1 cut(s) 1284
Eco31I GGTCTC 2 cut(s) 831, 1079
Eco47I GGWCC 6 cut(s) 602, 917, 1196, 1250, 1288, 1403
Eco57I CTGAAG 1 cut(s) 387
Eco81I CCTNAGG 1 cut(s) 1599
Eco88I CYCGRG 1 cut(s) 792
EcoO109I RGGNCCY 1 cut(s) 1196
EcoRI GAATTC 2 cut(s) 452, 735
EcoT14I CCWWGG 1 cut(s) 1284
EcoT22I ATGCAT 1 cut(s) 1438
ErhI CCWWGG 1 cut(s) 1284
FalI AAGNNNNNCTT 1 cut(s) 1645
FaqI GGGAC 3 cut(s) 494, 1006, 1603
FauI CCCGC 1 cut(s) 1521
FbaI TGATCA 2 cut(s) 106, 417
Fnu4HI GCNGC 2 cut(s) 216, 1646
FokI GGATG 5 cut(s) 152, 264, 1266, 1506, 1552
Fsp4HI GCNGC 2 cut(s) 216, 1646
FspBI CTAG 2 cut(s) 24, 1095
GluI GCNGC 2 cut(s) 216, 1646
HaeIII GGCC 2 cut(s) 89, 1604
HapII CCGG 2 cut(s) 1139, 1586
HgaI GACGC 1 cut(s) 1571
HincII GTYRAC 1 cut(s) 1561
HindII GTYRAC 1 cut(s) 1561
HindIII AAGCTT 3 cut(s) 1220, 1368, 1386
HinfI GANTC 6 cut(s) 615, 776, 812, 886, 1173, 1539
HpaII CCGG 2 cut(s) 1139, 1586
HphI GGTGA 1 cut(s) 950
Hpy166II GTNNAC 2 cut(s) 958, 1561
Hpy188I TCNGA 6 cut(s) 309, 367, 389, 867, 1237, 1505
Hpy188III TCNNGA 6 cut(s) 37, 110, 251, 468, 751, 1133
Hpy8I GTNNAC 2 cut(s) 958, 1561
Hpy99I CGWCG 1 cut(s) 1378
HpyAV CCTTC 4 cut(s) 130, 293, 387, 669
HpyCH4III ACNGT 8 cut(s) 76, 156, 374, 526, 748, 1048, 1124, 1465
HpyCH4IV ACGT 1 cut(s) 550
HpyCH4V TGCA 7 cut(s) 101, 275, 322, 872, 1277, 1436, 1648
HpyF10VI GCNNNNNNNGC 1 cut(s) 272
HpyF3I CTNAG 6 cut(s) 308, 866, 891, 905, 921, 1599
HpySE526I ACGT 1 cut(s) 550
Ksp22I TGATCA 2 cut(s) 106, 417
Kzo9I GATC 7 cut(s) 106, 148, 339, 417, 625, 1135, 1655
LmnI GCTCC 2 cut(s) 271, 686
Lsp1109I GCAGC 2 cut(s) 227, 1632
LweI GCATC 6 cut(s) 130, 1423, 1445, 1484, 1574, 1632
MaeI CTAG 2 cut(s) 24, 1095
MaeII ACGT 1 cut(s) 550
MaeIII GTNAC 2 cut(s) 546, 1344
MalI GATC 7 cut(s) 108, 150, 341, 419, 627, 1137, 1657
MboI GATC 7 cut(s) 106, 148, 339, 417, 625, 1135, 1655
MfeI CAATTG 1 cut(s) 879
MflI RGATCY 1 cut(s) 1655
MhlI GDGCHC 2 cut(s) 268, 1382
MlsI TGGCCA 1 cut(s) 89
MluNI TGGCCA 1 cut(s) 89
MlyI GAGTC 2 cut(s) 895, 1167
MmeI TCCRAC 4 cut(s) 138, 567, 1430, 1483
Mox20I TGGCCA 1 cut(s) 89
Mph1103I ATGCAT 1 cut(s) 1438
MroXI GAANNNNTTC 1 cut(s) 303
MscI TGGCCA 1 cut(s) 89
MslI CAYNNNNRTG 3 cut(s) 282, 1610, 1640
Msp20I TGGCCA 1 cut(s) 89
MspI CCGG 2 cut(s) 1139, 1586
MspR9I CCNGG 1 cut(s) 1587
MunI CAATTG 1 cut(s) 879
MwoI GCNNNNNNNGC 1 cut(s) 272
NciI CCSGG 1 cut(s) 1587
NdeII GATC 7 cut(s) 106, 148, 339, 417, 625, 1135, 1655
NmuCI GTSAC 2 cut(s) 546, 1344
NsiI ATGCAT 1 cut(s) 1438
NspI RCATGY 1 cut(s) 1102
PaeI GCATGC 1 cut(s) 1102
PaeR7I CTCGAG 1 cut(s) 792
PagI TCATGA 1 cut(s) 109
PcsI WCGNNNNNNNCGW 1 cut(s) 1065
PdmI GAANNNNTTC 1 cut(s) 303
PfeI GAWTC 4 cut(s) 615, 776, 812, 1539
PflMI CCANNNNNTGG 1 cut(s) 611
PkrI GCNGC 2 cut(s) 217, 1647
PleI GAGTC 2 cut(s) 894, 1167
PpsI GAGTC 2 cut(s) 894, 1167
PpuMI RGGWCCY 1 cut(s) 1196
PshAI GACNNNNGTC 1 cut(s) 1201
Psp5II RGGWCCY 1 cut(s) 1196
PspPI GGNCC 6 cut(s) 602, 917, 1196, 1250, 1288, 1403
PspPPI RGGWCCY 1 cut(s) 1196
PspXI VCTCGAGB 1 cut(s) 792
PsrI GAACNNNNNNTAC 2 cut(s) 1347, 1379
PsuI RGATCY 1 cut(s) 1655
RsaI GTAC 3 cut(s) 51, 1213, 1628
RsaNI GTAC 3 cut(s) 50, 1212, 1627
RseI CAYNNNNRTG 3 cut(s) 282, 1610, 1640
SatI GCNGC 2 cut(s) 216, 1646
Sau3AI GATC 7 cut(s) 106, 148, 339, 417, 625, 1135, 1655
Sau96I GGNCC 6 cut(s) 602, 917, 1196, 1250, 1288, 1403
SchI GAGTC 2 cut(s) 895, 1167
ScrFI CCNGG 1 cut(s) 1587
SduI GDGCHC 2 cut(s) 268, 1382
SfaNI GCATC 6 cut(s) 130, 1423, 1445, 1484, 1574, 1632
SfcI CTRYAG 2 cut(s) 1034, 1120
Sfr274I CTCGAG 1 cut(s) 792
SinI GGWCC 6 cut(s) 602, 917, 1196, 1250, 1288, 1403
SlaI CTCGAG 1 cut(s) 792
SmiMI CAYNNNNRTG 3 cut(s) 282, 1610, 1640
SmlI CTYRAG 2 cut(s) 409, 792
SmoI CTYRAG 2 cut(s) 409, 792
SphI GCATGC 1 cut(s) 1102
SsiI CCGC 5 cut(s) 651, 909, 1261, 1491, 1528
SspMI CTAG 2 cut(s) 24, 1095
StyD4I CCNGG 1 cut(s) 1585
StyI CCWWGG 1 cut(s) 1284
TaaI ACNGT 8 cut(s) 76, 156, 374, 526, 748, 1048, 1124, 1465
TaiI ACGT 1 cut(s) 553
TaqI TCGA 5 cut(s) 11, 750, 793, 1376, 1515
TatI WGTACW 1 cut(s) 49
TfiI GAWTC 4 cut(s) 615, 776, 812, 1539
TscAI CASTG 6 cut(s) 151, 515, 529, 979, 1129, 1353
TseFI GTSAC 2 cut(s) 546, 1344
TseI GCWGC 2 cut(s) 215, 1645
Tsp45I GTSAC 2 cut(s) 546, 1344
TspDTI ATGAA 8 cut(s) 68, 72, 84, 140, 728, 997, 1328, 1433
TspGWI ACGGA 1 cut(s) 699
TspRI CASTG 6 cut(s) 151, 515, 529, 979, 1129, 1353
Van91I CCANNNNNTGG 1 cut(s) 611
VpaK11BI GGWCC 6 cut(s) 602, 917, 1196, 1250, 1288, 1403
XapI RAATTY 4 cut(s) 452, 735, 1159, 1448
XceI RCATGY 1 cut(s) 1102
XcmI CCANNNNNNNNNTGG 1 cut(s) 971
XhoI CTCGAG 1 cut(s) 792
XmnI GAANNNNTTC 1 cut(s) 303
XspI CTAG 2 cut(s) 24, 1095
Zsp2I ATGCAT 1 cut(s) 1438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.