MD16G1093000.v1.1

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000533
Physical Location & Seq
Reverse (-)
6415965 .. 6417024
1060 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1093000.v1.1.491

Sequence Viewer

Length: 939 bp
ATGCACTATTCTCTAATGCAGGTTTGTCAGCTACAGGAGTCATATACAGTGATTCTAACGGGACATCTCACCGGGGTATTTGCAAACGCAGAAGTTATATTGAGTGCAGGAACACTTGGGACCCCTCAACTTCTACTAATAAGTGGCATTGGCCCAGAATCTTATCTATCATCTTTGAATATCACAGTTGTTCGTGATCATCCTTTCGTTGGGCAATTTGTTTATGACAATCCTCGTAATTTCATTAACATTGTGCCTCCAAATCCAATAGAAGCCTCAATTGTTACGGTTCTAGGCATTACAGATTATTTCTACCAATGTTCTCTCTCGAGCATGCCACTTACTACTCCAGCCTACAGCCTTTTTCCCACTCCGTTGGTTGTAAATTCAACTTTCACCCACATTCCTAGCAAAATTCCAGGACCTTTGTCTCATGGTTCTCTCACGTTGAATTCATCCTCTGATGTGAGATTCAGTCCAAATGTCAGATTCAACTACTTCTTAGATCCAACGGACCTTGCTCATTGTGTTAGCGCCATGAAAAATACGGGTGATTTATTAATGACAGACGCATTAAAACCATATAAAGCCCAAGATTTGCCGGGCATGGAAGGCTTCAACTTTTTGGGACAACCATTGCCAAAGAACCAAACAGATGATGCATCCTTCGAAACATTTTGTCGAGATACAGTAGCCTCGTATTGGCATTACCACGGTGGATGCCTTGTCGGGAAGGTGGTTGACTGCAGTTTCCGCCTTAATGGGATCTACGGATTACGCGTTGCTGATGCCACCCTATTTCCTGCTGCACCAGCAAGCCACCCTCAGGTATTCTATTTGATGTTGGGAAGGTACGTGGGACTTCAAATTCTGAAAGAAAGGTCAACTTGGGGAAACTCACTAACTCGGCATCATCCTGTGCCTTTTAAACTTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko01110 map00460 map01110
Pfam Domains
Protein Families
GMC_oxred_C GMC_oxred_N

Protein Analysis

313

Amino Acids

34.37

Weight (kDa)

6.58

Isoelectric Point (pI)

36.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_C PF05199 142 - 283 5.3e-23 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 10
AccII CGCG 1 cut(s) 780
AciI CCGC 1 cut(s) 754
AclWI GGATC 2 cut(s) 500, 773
AcsI RAATTY 4 cut(s) 385, 414, 451, 867
AfaI GTAC 1 cut(s) 854
AfiI CCNNNNNNNGG 3 cut(s) 209, 702, 826
AflII CTTAAG 1 cut(s) 932
AflIII ACRYGT 1 cut(s) 778
AgsI TTSAA 6 cut(s) 178, 390, 451, 493, 619, 866
AjnI CCWGG 1 cut(s) 418
AluBI AGCT 1 cut(s) 31
AluI AGCT 1 cut(s) 31
Alw26I GTCTC 1 cut(s) 435
AlwI GGATC 2 cut(s) 500, 773
Ama87I CYCGRG 1 cut(s) 328
AoxI GGCC 1 cut(s) 151
ApeKI GCWGC 1 cut(s) 806
ApoI RAATTY 4 cut(s) 385, 414, 451, 867
AseI ATTAAT 1 cut(s) 560
AspLEI GCGC 1 cut(s) 536
AspS9I GGNCC 4 cut(s) 120, 152, 422, 514
AsuC2I CCSGG 2 cut(s) 73, 603
AsuHPI GGTGA 3 cut(s) 61, 388, 563
AsuII TTCGAA 1 cut(s) 669
AvaI CYCGRG 1 cut(s) 328
AvaII GGWCC 3 cut(s) 120, 422, 514
AxyI CCTNAGG 1 cut(s) 825
BbvI GCAGC 1 cut(s) 793
BciT130I CCWGG 1 cut(s) 420
BclI TGATCA 1 cut(s) 196
BcnI CCSGG 2 cut(s) 73, 603
BcoDI GTCTC 1 cut(s) 435
BfaI CTAG 2 cut(s) 293, 408
BfmI CTRYAG 3 cut(s) 32, 355, 745
BfoI RGCGCY 1 cut(s) 537
BfrI CTTAAG 1 cut(s) 932
BfuAI ACCTGC 1 cut(s) 10
BisI GCNGC 1 cut(s) 807
BlsI GCNGC 1 cut(s) 808
Bme1390I CCNGG 3 cut(s) 73, 420, 603
Bme18I GGWCC 3 cut(s) 120, 422, 514
BmeT110I CYCGRG 1 cut(s) 328
BmgT120I GGNCC 4 cut(s) 120, 152, 422, 514
BmiI GGNNCC 2 cut(s) 121, 122
BmrFI CCNGG 3 cut(s) 73, 420, 603
BmsI GCATC 5 cut(s) 649, 671, 710, 778, 919
BoxI GACNNNNGTC 1 cut(s) 427
BpmI CTGGAG 1 cut(s) 333
Bpu14I TTCGAA 1 cut(s) 669
BpuMI CCSGG 2 cut(s) 73, 603
BsaAI YACGTR 1 cut(s) 856
BsaJI CCNNGG 2 cut(s) 72, 712
Bsc4I CCNNNNNNNGG 3 cut(s) 209, 702, 826
Bse21I CCTNAGG 1 cut(s) 825
Bse3DI GCAATG 1 cut(s) 635
BseBI CCWGG 1 cut(s) 420
BseDI CCNNGG 2 cut(s) 72, 712
BseGI GGATG 5 cut(s) 199, 455, 662, 725, 913
BseLI CCNNNNNNNGG 3 cut(s) 209, 702, 826
BseMI GCAATG 1 cut(s) 635
BseMII CTCAG 1 cut(s) 839
BseXI GCAGC 1 cut(s) 793
BsgI GTGCAG 2 cut(s) 126, 792
Bsh1236I CGCG 1 cut(s) 780
BshFI GGCC 1 cut(s) 153
BsiHKCI CYCGRG 1 cut(s) 328
BsiSI CCGG 2 cut(s) 72, 602
BslFI GGGAC 4 cut(s) 75, 133, 642, 873
BslI CCNNNNNNNGG 3 cut(s) 209, 702, 826
BsmAI GTCTC 1 cut(s) 435
BsmFI GGGAC 4 cut(s) 75, 133, 642, 873
BsnI GGCC 1 cut(s) 153
BsoBI CYCGRG 1 cut(s) 328
Bsp119I TTCGAA 1 cut(s) 669
Bsp143I GATC 3 cut(s) 196, 505, 765
BspACI CCGC 1 cut(s) 754
BspANI GGCC 1 cut(s) 153
BspCNI CTCAG 1 cut(s) 838
BspFNI CGCG 1 cut(s) 780
BspLI GGNNCC 2 cut(s) 121, 122
BspMAI CTGCAG 1 cut(s) 749
BspMI ACCTGC 1 cut(s) 10
BspPI GGATC 2 cut(s) 500, 773
BspT104I TTCGAA 1 cut(s) 669
BspTI CTTAAG 1 cut(s) 932
BsrDI GCAATG 1 cut(s) 635
BssECI CCNNGG 2 cut(s) 72, 712
BssMI GATC 3 cut(s) 196, 505, 765
Bst2UI CCWGG 1 cut(s) 420
Bst4CI ACNGT 5 cut(s) 49, 187, 289, 691, 716
BstAFI CTTAAG 1 cut(s) 932
BstBAI YACGTR 1 cut(s) 856
BstBI TTCGAA 1 cut(s) 669
BstC8I GCNNGC 2 cut(s) 335, 817
BstDEI CTNAG 2 cut(s) 502, 825
BstDSI CCRYGG 1 cut(s) 712
BstF5I GGATG 5 cut(s) 199, 455, 662, 725, 913
BstFNI CGCG 1 cut(s) 780
BstH2I RGCGCY 1 cut(s) 537
BstHHI GCGC 1 cut(s) 536
BstKTI GATC 3 cut(s) 199, 508, 768
BstMAI GTCTC 1 cut(s) 435
BstMBI GATC 3 cut(s) 196, 505, 765
BstMWI GCNNNNNNNGC 3 cut(s) 612, 753, 812
BstNI CCWGG 1 cut(s) 420
BstNSI RCATGY 1 cut(s) 337
BstPAI GACNNNNGTC 1 cut(s) 427
BstSCI CCNGG 3 cut(s) 71, 418, 601
BstSFI CTRYAG 3 cut(s) 32, 355, 745
BstUI CGCG 1 cut(s) 780
BstV1I GCAGC 1 cut(s) 793
BstX2I RGATCY 2 cut(s) 505, 765
BstXI CCANNNNNNTGG 1 cut(s) 376
BstYI RGATCY 2 cut(s) 505, 765
Bsu36I CCTNAGG 1 cut(s) 825
BsuRI GGCC 1 cut(s) 153
BtgI CCRYGG 1 cut(s) 712
BtsCI GGATG 5 cut(s) 199, 455, 662, 725, 913
BtsIMutI CAGTG 1 cut(s) 54
BveI ACCTGC 1 cut(s) 10
Cac8I GCNNGC 2 cut(s) 335, 817
CfoI GCGC 1 cut(s) 536
Cfr13I GGNCC 4 cut(s) 120, 152, 422, 514
CseI GACGC 1 cut(s) 578
Csp6I GTAC 1 cut(s) 853
CviAII CATG 4 cut(s) 334, 434, 538, 607
CviJI RGCY 9 cut(s) 31, 153, 275, 353, 360, 590, 615, 695, 819
CviKI_1 RGCY 9 cut(s) 31, 153, 275, 353, 360, 590, 615, 695, 819
CviQI GTAC 1 cut(s) 853
DdeI CTNAG 2 cut(s) 502, 825
DpnI GATC 3 cut(s) 198, 507, 767
DpnII GATC 3 cut(s) 196, 505, 765
DraI TTTAAA 1 cut(s) 928
EciI GGCGGA 1 cut(s) 743
Eco47I GGWCC 3 cut(s) 120, 422, 514
Eco81I CCTNAGG 1 cut(s) 825
Eco88I CYCGRG 1 cut(s) 328
EcoO109I RGGNCCY 2 cut(s) 120, 422
EcoRI GAATTC 1 cut(s) 451
EcoRII CCWGG 1 cut(s) 418
EcoT22I ATGCAT 1 cut(s) 664
FaeI CATG 4 cut(s) 337, 437, 541, 610
FalI AAGNNNNNCTT 2 cut(s) 871, 903
FaqI GGGAC 4 cut(s) 75, 133, 642, 873
FatI CATG 4 cut(s) 333, 433, 537, 606
FbaI TGATCA 1 cut(s) 196
Fnu4HI GCNGC 1 cut(s) 807
FokI GGATG 5 cut(s) 186, 442, 649, 732, 900
Fsp4HI GCNGC 1 cut(s) 807
FspBI CTAG 2 cut(s) 293, 408
GlaI GCGC 1 cut(s) 535
GluI GCNGC 1 cut(s) 807
GsuI CTGGAG 1 cut(s) 333
HaeII RGCGCY 1 cut(s) 537
HaeIII GGCC 1 cut(s) 153
HapII CCGG 2 cut(s) 72, 602
HgaI GACGC 1 cut(s) 578
HhaI GCGC 1 cut(s) 536
Hin1II CATG 4 cut(s) 337, 437, 541, 610
Hin6I GCGC 1 cut(s) 534
HinP1I GCGC 1 cut(s) 534
HincII GTYRAC 2 cut(s) 742, 885
HindII GTYRAC 2 cut(s) 742, 885
HinfI GANTC 5 cut(s) 38, 52, 158, 471, 489
HpaII CCGG 2 cut(s) 72, 602
HphI GGTGA 3 cut(s) 61, 388, 563
Hpy166II GTNNAC 2 cut(s) 742, 885
Hpy188I TCNGA 3 cut(s) 463, 488, 873
Hpy188III TCNNGA 4 cut(s) 194, 328, 683, 730
Hpy8I GTNNAC 2 cut(s) 742, 885
HpyAV CCTTC 4 cut(s) 605, 676, 727, 843
HpyCH4III ACNGT 5 cut(s) 49, 187, 289, 691, 716
HpyCH4IV ACGT 2 cut(s) 446, 855
HpyCH4V TGCA 7 cut(s) 4, 19, 83, 107, 662, 747, 809
HpyF10VI GCNNNNNNNGC 3 cut(s) 612, 753, 812
HpyF3I CTNAG 2 cut(s) 502, 825
HpySE526I ACGT 2 cut(s) 446, 855
Hsp92II CATG 4 cut(s) 337, 437, 541, 610
HspAI GCGC 1 cut(s) 534
KflI GGGWCCC 1 cut(s) 120
Ksp22I TGATCA 1 cut(s) 196
Kzo9I GATC 3 cut(s) 196, 505, 765
Lsp1109I GCAGC 1 cut(s) 793
LweI GCATC 5 cut(s) 649, 671, 710, 778, 919
MaeI CTAG 2 cut(s) 293, 408
MaeII ACGT 2 cut(s) 446, 855
MaeIII GTNAC 1 cut(s) 283
MalI GATC 3 cut(s) 198, 507, 767
MboI GATC 3 cut(s) 196, 505, 765
MfeI CAATTG 1 cut(s) 279
MflI RGATCY 2 cut(s) 505, 765
MluCI AATT 7 cut(s) 215, 238, 279, 385, 414, 451, 867
MluI ACGCGT 1 cut(s) 778
MlyI GAGTC 1 cut(s) 47
MmeI TCCRAC 1 cut(s) 533
MnlI CCTC 7 cut(s) 135, 243, 267, 286, 469, 706, 834
Mph1103I ATGCAT 1 cut(s) 664
MseI TTAA 6 cut(s) 246, 560, 575, 759, 927, 933
MspCI CTTAAG 1 cut(s) 932
MspI CCGG 2 cut(s) 72, 602
MspR9I CCNGG 3 cut(s) 73, 420, 603
MunI CAATTG 1 cut(s) 279
MvaI CCWGG 1 cut(s) 420
MvnI CGCG 1 cut(s) 780
MwoI GCNNNNNNNGC 3 cut(s) 612, 753, 812
NciI CCSGG 2 cut(s) 73, 603
NdeII GATC 3 cut(s) 196, 505, 765
NlaIII CATG 4 cut(s) 337, 437, 541, 610
NlaIV GGNNCC 2 cut(s) 121, 122
NmeAIII GCCGAG 1 cut(s) 886
NsiI ATGCAT 1 cut(s) 664
NspI RCATGY 1 cut(s) 337
NspV TTCGAA 1 cut(s) 669
PaeI GCATGC 1 cut(s) 337
PaeR7I CTCGAG 1 cut(s) 328
PcsI WCGNNNNNNNCGW 1 cut(s) 777
PfeI GAWTC 4 cut(s) 52, 158, 471, 489
PfoI TCCNGGA 1 cut(s) 418
PkrI GCNGC 1 cut(s) 808
PleI GAGTC 1 cut(s) 46
PpsI GAGTC 1 cut(s) 46
Ppu21I YACGTR 1 cut(s) 856
PpuMI RGGWCCY 2 cut(s) 120, 422
PshAI GACNNNNGTC 1 cut(s) 427
PshBI ATTAAT 1 cut(s) 560
Psp5II RGGWCCY 2 cut(s) 120, 422
Psp6I CCWGG 1 cut(s) 418
PspGI CCWGG 1 cut(s) 418
PspN4I GGNNCC 2 cut(s) 121, 122
PspPI GGNCC 4 cut(s) 120, 152, 422, 514
PspPPI RGGWCCY 2 cut(s) 120, 422
PstI CTGCAG 1 cut(s) 749
PsuI RGATCY 2 cut(s) 505, 765
RsaI GTAC 1 cut(s) 854
RsaNI GTAC 1 cut(s) 853
SaqAI TTAA 6 cut(s) 246, 560, 575, 759, 927, 933
SatI GCNGC 1 cut(s) 807
Sau3AI GATC 3 cut(s) 196, 505, 765
Sau96I GGNCC 4 cut(s) 120, 152, 422, 514
SchI GAGTC 1 cut(s) 47
ScrFI CCNGG 3 cut(s) 73, 420, 603
SfaNI GCATC 5 cut(s) 649, 671, 710, 778, 919
SfcI CTRYAG 3 cut(s) 32, 355, 745
Sfr274I CTCGAG 1 cut(s) 328
SfuI TTCGAA 1 cut(s) 669
SinI GGWCC 3 cut(s) 120, 422, 514
SlaI CTCGAG 1 cut(s) 328
SmlI CTYRAG 2 cut(s) 328, 932
SmoI CTYRAG 2 cut(s) 328, 932
SphI GCATGC 1 cut(s) 337
Sse9I AATT 7 cut(s) 215, 238, 279, 385, 414, 451, 867
SsiI CCGC 1 cut(s) 754
SspMI CTAG 2 cut(s) 293, 408
StyD4I CCNGG 3 cut(s) 71, 418, 601
TaaI ACNGT 5 cut(s) 49, 187, 289, 691, 716
TaiI ACGT 2 cut(s) 449, 858
TaqI TCGA 3 cut(s) 329, 669, 682
TasI AATT 7 cut(s) 215, 238, 279, 385, 414, 451, 867
TfiI GAWTC 4 cut(s) 52, 158, 471, 489
Tru1I TTAA 6 cut(s) 246, 560, 575, 759, 927, 933
Tru9I TTAA 6 cut(s) 246, 560, 575, 759, 927, 933
TscAI CASTG 1 cut(s) 54
TseI GCWGC 1 cut(s) 806
TspDTI ATGAA 3 cut(s) 232, 444, 554
TspGWI ACGGA 3 cut(s) 363, 527, 786
TspRI CASTG 1 cut(s) 54
Vha464I CTTAAG 1 cut(s) 932
VpaK11BI GGWCC 3 cut(s) 120, 422, 514
VspI ATTAAT 1 cut(s) 560
XapI RAATTY 4 cut(s) 385, 414, 451, 867
XceI RCATGY 1 cut(s) 337
XhoI CTCGAG 1 cut(s) 328
XspI CTAG 2 cut(s) 293, 408
Zsp2I ATGCAT 1 cut(s) 664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.