(R)-mandelonitrile lyase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 845 |
| Acc36I | ACCTGC | 2 cut(s) | 845, 1596 |
| AccB1I | GGYRCC | 1 cut(s) | 195 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 593, 1458 |
| AciI | CCGC | 2 cut(s) | 1388, 1510 |
| AclWI | GGATC | 4 cut(s) | 615, 746, 1529, 1555 |
| AcsI | RAATTY | 7 cut(s) | 119, 304, 440, 529, 1141, 1158, 1244 |
| AcyI | GRCGYC | 1 cut(s) | 332 |
| AfaI | GTAC | 2 cut(s) | 41, 126 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 65, 394, 593, 956, 1458 |
| AflIII | ACRYGT | 1 cut(s) | 1534 |
| AgsI | TTSAA | 9 cut(s) | 118, 506, 1013, 1146, 1249, 1333, 1375, 1558, 1622 |
| AjnI | CCWGG | 4 cut(s) | 64, 1174, 1357, 1567 |
| AluBI | AGCT | 5 cut(s) | 111, 368, 575, 671, 773 |
| AluI | AGCT | 5 cut(s) | 111, 368, 575, 671, 773 |
| Alw21I | GWGCWC | 2 cut(s) | 237, 862 |
| Alw26I | GTCTC | 2 cut(s) | 813, 1061 |
| Alw44I | GTGCAC | 1 cut(s) | 858 |
| AlwI | GGATC | 4 cut(s) | 615, 746, 1529, 1555 |
| Ama87I | CYCGRG | 1 cut(s) | 1347 |
| AoxI | GGCC | 3 cut(s) | 336, 391, 898 |
| ApaLI | GTGCAC | 1 cut(s) | 858 |
| ApeKI | GCWGC | 1 cut(s) | 20 |
| ApoI | RAATTY | 7 cut(s) | 119, 304, 440, 529, 1141, 1158, 1244 |
| AspA2I | CCTAGG | 1 cut(s) | 388 |
| AspS9I | GGNCC | 6 cut(s) | 337, 385, 899, 1178, 1385, 1572 |
| AsuC2I | CCSGG | 1 cut(s) | 395 |
| AsuHPI | GGTGA | 5 cut(s) | 449, 848, 932, 1319, 1557 |
| AvaI | CYCGRG | 1 cut(s) | 1347 |
| AvaII | GGWCC | 4 cut(s) | 385, 1178, 1385, 1572 |
| AvrII | CCTAGG | 1 cut(s) | 388 |
| BaeGI | GKGCMC | 3 cut(s) | 188, 200, 862 |
| BanI | GGYRCC | 1 cut(s) | 195 |
| Bbv12I | GWGCWC | 2 cut(s) | 237, 862 |
| BbvI | GCAGC | 1 cut(s) | 7 |
| BccI | CCATC | 5 cut(s) | 97, 268, 353, 559, 1538 |
| BceAI | ACGGC | 3 cut(s) | 204, 648, 1277 |
| BciT130I | CCWGG | 4 cut(s) | 66, 1176, 1359, 1569 |
| BclI | TGATCA | 1 cut(s) | 405 |
| BcnI | CCSGG | 1 cut(s) | 395 |
| BcoDI | GTCTC | 2 cut(s) | 813, 1061 |
| BfaI | CTAG | 4 cut(s) | 242, 389, 1116, 1364 |
| BfmI | CTRYAG | 1 cut(s) | 513 |
| BfuAI | ACCTGC | 2 cut(s) | 845, 1596 |
| BglII | AGATCT | 1 cut(s) | 1098 |
| BisI | GCNGC | 1 cut(s) | 21 |
| BlnI | CCTAGG | 1 cut(s) | 388 |
| BlsI | GCNGC | 1 cut(s) | 22 |
| Bme1390I | CCNGG | 5 cut(s) | 66, 395, 1176, 1359, 1569 |
| Bme18I | GGWCC | 4 cut(s) | 385, 1178, 1385, 1572 |
| BmeT110I | CYCGRG | 1 cut(s) | 1347 |
| BmgT120I | GGNCC | 6 cut(s) | 337, 385, 899, 1178, 1385, 1572 |
| BmiI | GGNNCC | 5 cut(s) | 72, 197, 386, 1180, 1573 |
| BmrFI | CCNGG | 5 cut(s) | 66, 395, 1176, 1359, 1569 |
| BmrI | ACTGGG | 1 cut(s) | 926 |
| BmsI | GCATC | 5 cut(s) | 550, 767, 1405, 1427, 1466 |
| BmuI | ACTGGG | 1 cut(s) | 926 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1058, 1090 |
| BpmI | CTGGAG | 1 cut(s) | 1158 |
| BpuEI | CTTGAG | 4 cut(s) | 409, 868, 1033, 1566 |
| BpuMI | CCSGG | 1 cut(s) | 395 |
| BsaAI | YACGTR | 3 cut(s) | 128, 827, 1535 |
| BsaHI | GRCGYC | 1 cut(s) | 332 |
| BsaI | GGTCTC | 1 cut(s) | 813 |
| BsaJI | CCNNGG | 5 cut(s) | 65, 388, 394, 1468, 1568 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1464, 1494 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 65, 394, 593, 956, 1458 |
| Bse118I | RCCGGY | 1 cut(s) | 334 |
| Bse1I | ACTGG | 3 cut(s) | 545, 932, 1405 |
| BseBI | CCWGG | 4 cut(s) | 66, 1176, 1359, 1569 |
| BseDI | CCNNGG | 5 cut(s) | 65, 388, 394, 1468, 1568 |
| BseGI | GGATG | 4 cut(s) | 694, 1211, 1418, 1481 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 65, 394, 593, 956, 1458 |
| BseMII | CTCAG | 2 cut(s) | 698, 1109 |
| BseNI | ACTGG | 3 cut(s) | 545, 932, 1405 |
| BseSI | GKGCMC | 3 cut(s) | 188, 200, 862 |
| BseXI | GCAGC | 1 cut(s) | 7 |
| BsgI | GTGCAG | 1 cut(s) | 873 |
| Bsh1285I | CGRYCG | 1 cut(s) | 652 |
| BshFI | GGCC | 3 cut(s) | 338, 393, 900 |
| BshNI | GGYRCC | 1 cut(s) | 195 |
| BsiEI | CGRYCG | 1 cut(s) | 652 |
| BsiHKAI | GWGCWC | 2 cut(s) | 237, 862 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1347 |
| BsiSI | CCGG | 3 cut(s) | 335, 394, 1077 |
| BslFI | GGGAC | 2 cut(s) | 307, 1585 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 65, 394, 593, 956, 1458 |
| BsmAI | GTCTC | 2 cut(s) | 813, 1061 |
| BsmFI | GGGAC | 2 cut(s) | 307, 1585 |
| BsmI | GAATGC | 1 cut(s) | 518 |
| BsnI | GGCC | 3 cut(s) | 338, 393, 900 |
| Bso31I | GGTCTC | 1 cut(s) | 813 |
| BsoBI | CYCGRG | 1 cut(s) | 1347 |
| Bsp1286I | GDGCHC | 4 cut(s) | 188, 200, 237, 862 |
| Bsp1407I | TGTACA | 1 cut(s) | 39 |
| Bsp143I | GATC | 9 cut(s) | 139, 405, 607, 649, 738, 1098, 1521, 1547, 1637 |
| Bsp19I | CCATGG | 1 cut(s) | 1468 |
| BspACI | CCGC | 2 cut(s) | 1388, 1510 |
| BspANI | GGCC | 3 cut(s) | 338, 393, 900 |
| BspCNI | CTCAG | 2 cut(s) | 699, 1108 |
| BspLI | GGNNCC | 5 cut(s) | 72, 197, 386, 1180, 1573 |
| BspMI | ACCTGC | 2 cut(s) | 845, 1596 |
| BspPI | GGATC | 4 cut(s) | 615, 746, 1529, 1555 |
| BspT107I | GGYRCC | 1 cut(s) | 195 |
| BspTNI | GGTCTC | 1 cut(s) | 813 |
| BsrFI | RCCGGY | 1 cut(s) | 334 |
| BsrGI | TGTACA | 1 cut(s) | 39 |
| BsrI | ACTGG | 3 cut(s) | 545, 932, 1405 |
| BssAI | RCCGGY | 1 cut(s) | 334 |
| BssECI | CCNNGG | 5 cut(s) | 65, 388, 394, 1468, 1568 |
| BssMI | GATC | 9 cut(s) | 139, 405, 607, 649, 738, 1098, 1521, 1547, 1637 |
| BssNI | GRCGYC | 1 cut(s) | 332 |
| BssT1I | CCWWGG | 2 cut(s) | 388, 1468 |
| Bst2UI | CCWGG | 4 cut(s) | 66, 1176, 1359, 1569 |
| Bst4CI | ACNGT | 9 cut(s) | 326, 437, 514, 604, 730, 791, 1338, 1370, 1447 |
| Bst6I | CTCTTC | 1 cut(s) | 754 |
| BstACI | GRCGYC | 1 cut(s) | 332 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 722, 967 |
| BstAUI | TGTACA | 1 cut(s) | 39 |
| BstBAI | YACGTR | 3 cut(s) | 128, 827, 1535 |
| BstC8I | GCNNGC | 4 cut(s) | 285, 336, 577, 1082 |
| BstDEI | CTNAG | 4 cut(s) | 478, 707, 887, 1095 |
| BstDSI | CCRYGG | 1 cut(s) | 1468 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 63 |
| BstF5I | GGATG | 4 cut(s) | 694, 1211, 1418, 1481 |
| BstKTI | GATC | 9 cut(s) | 142, 408, 610, 652, 741, 1101, 1524, 1550, 1640 |
| BstMAI | GTCTC | 2 cut(s) | 813, 1061 |
| BstMBI | GATC | 9 cut(s) | 139, 405, 607, 649, 738, 1098, 1521, 1547, 1637 |
| BstMCI | CGRYCG | 1 cut(s) | 652 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 722, 967, 1265 |
| BstNI | CCWGG | 4 cut(s) | 66, 1176, 1359, 1569 |
| BstSCI | CCNGG | 5 cut(s) | 64, 393, 1174, 1357, 1567 |
| BstSFI | CTRYAG | 1 cut(s) | 513 |
| BstSLI | GKGCMC | 3 cut(s) | 188, 200, 862 |
| BstSNI | TACGTA | 1 cut(s) | 128 |
| BstV1I | GCAGC | 1 cut(s) | 7 |
| BstX2I | RGATCY | 2 cut(s) | 1098, 1547 |
| BstYI | RGATCY | 2 cut(s) | 1098, 1547 |
| BsuRI | GGCC | 3 cut(s) | 338, 393, 900 |
| BtgI | CCRYGG | 1 cut(s) | 1468 |
| BtgZI | GCGATG | 1 cut(s) | 578 |
| BtsCI | GGATG | 4 cut(s) | 694, 1211, 1418, 1481 |
| BtsIMutI | CAGTG | 3 cut(s) | 510, 538, 796 |
| BveI | ACCTGC | 2 cut(s) | 845, 1596 |
| Cac8I | GCNNGC | 4 cut(s) | 285, 336, 577, 1082 |
| Cfr10I | RCCGGY | 1 cut(s) | 334 |
| Cfr13I | GGNCC | 6 cut(s) | 337, 385, 899, 1178, 1385, 1572 |
| CseI | GACGC | 1 cut(s) | 340 |
| Csp6I | GTAC | 2 cut(s) | 40, 125 |
| CviAII | CATG | 5 cut(s) | 310, 472, 665, 1469, 1516 |
| CviQI | GTAC | 2 cut(s) | 40, 125 |
| DdeI | CTNAG | 4 cut(s) | 478, 707, 887, 1095 |
| DpnI | GATC | 9 cut(s) | 141, 407, 609, 651, 740, 1100, 1523, 1549, 1639 |
| DpnII | GATC | 9 cut(s) | 139, 405, 607, 649, 738, 1098, 1521, 1547, 1637 |
| Eam1104I | CTCTTC | 1 cut(s) | 754 |
| EarI | CTCTTC | 1 cut(s) | 754 |
| Eco105I | TACGTA | 1 cut(s) | 128 |
| Eco130I | CCWWGG | 2 cut(s) | 388, 1468 |
| Eco31I | GGTCTC | 1 cut(s) | 813 |
| Eco47I | GGWCC | 4 cut(s) | 385, 1178, 1385, 1572 |
| Eco88I | CYCGRG | 1 cut(s) | 1347 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 63 |
| EcoO109I | RGGNCCY | 2 cut(s) | 385, 1178 |
| EcoRI | GAATTC | 1 cut(s) | 440 |
| EcoRII | CCWGG | 4 cut(s) | 64, 1174, 1357, 1567 |
| EcoT14I | CCWWGG | 2 cut(s) | 388, 1468 |
| EcoT22I | ATGCAT | 1 cut(s) | 1420 |
| ErhI | CCWWGG | 2 cut(s) | 388, 1468 |
| FaeI | CATG | 5 cut(s) | 313, 475, 668, 1472, 1519 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1575, 1607 |
| FaqI | GGGAC | 2 cut(s) | 307, 1585 |
| FatI | CATG | 5 cut(s) | 309, 471, 664, 1468, 1515 |
| FauI | CCCGC | 1 cut(s) | 1503 |
| FauNDI | CATATG | 1 cut(s) | 493 |
| FbaI | TGATCA | 1 cut(s) | 405 |
| Fnu4HI | GCNGC | 1 cut(s) | 21 |
| FokI | GGATG | 4 cut(s) | 681, 1198, 1405, 1488 |
| Fsp4HI | GCNGC | 1 cut(s) | 21 |
| FspBI | CTAG | 4 cut(s) | 242, 389, 1116, 1364 |
| GluI | GCNGC | 1 cut(s) | 21 |
| GsuI | CTGGAG | 1 cut(s) | 1158 |
| HaeIII | GGCC | 3 cut(s) | 338, 393, 900 |
| HapII | CCGG | 3 cut(s) | 335, 394, 1077 |
| HgaI | GACGC | 1 cut(s) | 340 |
| Hin1I | GRCGYC | 1 cut(s) | 332 |
| Hin1II | CATG | 5 cut(s) | 313, 475, 668, 1472, 1519 |
| HinfI | GANTC | 2 cut(s) | 780, 794 |
| HpaII | CCGG | 3 cut(s) | 335, 394, 1077 |
| HphI | GGTGA | 5 cut(s) | 449, 848, 932, 1319, 1557 |
| Hpy166II | GTNNAC | 3 cut(s) | 348, 860, 940 |
| Hpy188I | TCNGA | 7 cut(s) | 94, 355, 528, 1098, 1411, 1487, 1642 |
| Hpy188III | TCNNGA | 8 cut(s) | 242, 426, 456, 587, 847, 1349, 1364, 1439 |
| Hpy8I | GTNNAC | 3 cut(s) | 348, 860, 940 |
| Hpy99I | CGWCG | 1 cut(s) | 437 |
| HpyAV | CCTTC | 1 cut(s) | 248 |
| HpyCH4III | ACNGT | 9 cut(s) | 326, 437, 514, 604, 730, 791, 1338, 1370, 1447 |
| HpyCH4IV | ACGT | 4 cut(s) | 127, 826, 990, 1534 |
| HpyCH4V | TGCA | 9 cut(s) | 221, 302, 313, 716, 854, 860, 1207, 1259, 1418 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 722, 967, 1265 |
| HpyF3I | CTNAG | 4 cut(s) | 478, 707, 887, 1095 |
| HpySE526I | ACGT | 4 cut(s) | 127, 826, 990, 1534 |
| Hsp92I | GRCGYC | 1 cut(s) | 332 |
| Hsp92II | CATG | 5 cut(s) | 313, 475, 668, 1472, 1519 |
| KroI | GCCGGC | 1 cut(s) | 334 |
| KroNI | GCCGGC | 1 cut(s) | 336 |
| Ksp22I | TGATCA | 1 cut(s) | 405 |
| Kzo9I | GATC | 9 cut(s) | 139, 405, 607, 649, 738, 1098, 1521, 1547, 1637 |
| LmnI | GCTCC | 3 cut(s) | 240, 668, 1237 |
| Lsp1109I | GCAGC | 1 cut(s) | 7 |
| LweI | GCATC | 5 cut(s) | 550, 767, 1405, 1427, 1466 |
| MaeI | CTAG | 4 cut(s) | 242, 389, 1116, 1364 |
| MaeII | ACGT | 4 cut(s) | 127, 826, 990, 1534 |
| MaeIII | GTNAC | 3 cut(s) | 128, 628, 991 |
| MalI | GATC | 9 cut(s) | 141, 407, 609, 651, 740, 1100, 1523, 1549, 1639 |
| MboI | GATC | 9 cut(s) | 139, 405, 607, 649, 738, 1098, 1521, 1547, 1637 |
| MboII | GAAGA | 6 cut(s) | 167, 329, 368, 741, 1514, 1643 |
| MflI | RGATCY | 2 cut(s) | 1098, 1547 |
| MhlI | GDGCHC | 4 cut(s) | 188, 200, 237, 862 |
| MmeI | TCCRAC | 2 cut(s) | 1145, 1465 |
| Mph1103I | ATGCAT | 1 cut(s) | 1420 |
| MroNI | GCCGGC | 1 cut(s) | 334 |
| MseI | TTAA | 6 cut(s) | 374, 462, 924, 1200, 1316, 1425 |
| MslI | CAYNNNNRTG | 4 cut(s) | 173, 496, 1467, 1592 |
| MspI | CCGG | 3 cut(s) | 335, 394, 1077 |
| MspR9I | CCNGG | 5 cut(s) | 66, 395, 1176, 1359, 1569 |
| Mva1269I | GAATGC | 1 cut(s) | 518 |
| MvaI | CCWGG | 4 cut(s) | 66, 1176, 1359, 1569 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 722, 967, 1265 |
| NaeI | GCCGGC | 1 cut(s) | 336 |
| NciI | CCSGG | 1 cut(s) | 395 |
| NcoI | CCATGG | 1 cut(s) | 1468 |
| NdeI | CATATG | 1 cut(s) | 493 |
| NdeII | GATC | 9 cut(s) | 139, 405, 607, 649, 738, 1098, 1521, 1547, 1637 |
| NgoMIV | GCCGGC | 1 cut(s) | 334 |
| NlaIII | CATG | 5 cut(s) | 313, 475, 668, 1472, 1519 |
| NlaIV | GGNNCC | 5 cut(s) | 72, 197, 386, 1180, 1573 |
| NmuCI | GTSAC | 1 cut(s) | 628 |
| NsiI | ATGCAT | 1 cut(s) | 1420 |
| PaeR7I | CTCGAG | 1 cut(s) | 1347 |
| PaqCI | CACCTGC | 1 cut(s) | 845 |
| PctI | GAATGC | 1 cut(s) | 518 |
| PdiI | GCCGGC | 1 cut(s) | 336 |
| PfeI | GAWTC | 2 cut(s) | 780, 794 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 593, 1458 |
| PfoI | TCCNGGA | 1 cut(s) | 1174 |
| PkrI | GCNGC | 1 cut(s) | 22 |
| Ple19I | CGATCG | 1 cut(s) | 652 |
| Ppu21I | YACGTR | 3 cut(s) | 128, 827, 1535 |
| PpuMI | RGGWCCY | 2 cut(s) | 385, 1178 |
| Psp5II | RGGWCCY | 2 cut(s) | 385, 1178 |
| Psp6I | CCWGG | 4 cut(s) | 64, 1174, 1357, 1567 |
| PspGI | CCWGG | 4 cut(s) | 64, 1174, 1357, 1567 |
| PspN4I | GGNNCC | 5 cut(s) | 72, 197, 386, 1180, 1573 |
| PspPI | GGNCC | 6 cut(s) | 337, 385, 899, 1178, 1385, 1572 |
| PspPPI | RGGWCCY | 2 cut(s) | 385, 1178 |
| PsuI | RGATCY | 2 cut(s) | 1098, 1547 |
| PvuI | CGATCG | 1 cut(s) | 652 |
| RsaI | GTAC | 2 cut(s) | 41, 126 |
| RsaNI | GTAC | 2 cut(s) | 40, 125 |
| RseI | CAYNNNNRTG | 4 cut(s) | 173, 496, 1467, 1592 |
| SaqAI | TTAA | 6 cut(s) | 374, 462, 924, 1200, 1316, 1425 |
| SatI | GCNGC | 1 cut(s) | 21 |
| Sau3AI | GATC | 9 cut(s) | 139, 405, 607, 649, 738, 1098, 1521, 1547, 1637 |
| Sau96I | GGNCC | 6 cut(s) | 337, 385, 899, 1178, 1385, 1572 |
| ScrFI | CCNGG | 5 cut(s) | 66, 395, 1176, 1359, 1569 |
| SduI | GDGCHC | 4 cut(s) | 188, 200, 237, 862 |
| SfaNI | GCATC | 5 cut(s) | 550, 767, 1405, 1427, 1466 |
| SfcI | CTRYAG | 1 cut(s) | 513 |
| Sfr274I | CTCGAG | 1 cut(s) | 1347 |
| SinI | GGWCC | 4 cut(s) | 385, 1178, 1385, 1572 |
| SlaI | CTCGAG | 1 cut(s) | 1347 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 173, 496, 1467, 1592 |
| SmlI | CTYRAG | 5 cut(s) | 424, 847, 1048, 1347, 1581 |
| SmoI | CTYRAG | 5 cut(s) | 424, 847, 1048, 1347, 1581 |
| SnaBI | TACGTA | 1 cut(s) | 128 |
| SsiI | CCGC | 2 cut(s) | 1388, 1510 |
| SspI | AATATT | 1 cut(s) | 256 |
| SspMI | CTAG | 4 cut(s) | 242, 389, 1116, 1364 |
| StyD4I | CCNGG | 5 cut(s) | 64, 393, 1174, 1357, 1567 |
| StyI | CCWWGG | 2 cut(s) | 388, 1468 |
| TaaI | ACNGT | 9 cut(s) | 326, 437, 514, 604, 730, 791, 1338, 1370, 1447 |
| TaiI | ACGT | 4 cut(s) | 130, 829, 993, 1537 |
| TaqI | TCGA | 2 cut(s) | 1348, 1438 |
| TatI | WGTACW | 1 cut(s) | 39 |
| TfiI | GAWTC | 2 cut(s) | 780, 794 |
| Tru1I | TTAA | 6 cut(s) | 374, 462, 924, 1200, 1316, 1425 |
| Tru9I | TTAA | 6 cut(s) | 374, 462, 924, 1200, 1316, 1425 |
| TscAI | CASTG | 3 cut(s) | 517, 545, 796 |
| TseFI | GTSAC | 1 cut(s) | 628 |
| TseI | GCWGC | 1 cut(s) | 20 |
| Tsp45I | GTSAC | 1 cut(s) | 628 |
| TspDTI | ATGAA | 7 cut(s) | 298, 480, 488, 510, 906, 1415, 1515 |
| TspGWI | ACGGA | 2 cut(s) | 220, 512 |
| TspRI | CASTG | 3 cut(s) | 517, 545, 796 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 593, 1458 |
| VneI | GTGCAC | 1 cut(s) | 858 |
| VpaK11BI | GGWCC | 4 cut(s) | 385, 1178, 1385, 1572 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 63 |
| XapI | RAATTY | 7 cut(s) | 119, 304, 440, 529, 1141, 1158, 1244 |
| XbaI | TCTAGA | 2 cut(s) | 241, 1363 |
| XhoI | CTCGAG | 1 cut(s) | 1347 |
| XmaJI | CCTAGG | 1 cut(s) | 388 |
| XspI | CTAG | 4 cut(s) | 242, 389, 1116, 1364 |
| Zsp2I | ATGCAT | 1 cut(s) | 1420 |