Prupe.1G093700_v2.0.a1

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000533
Physical Location & Seq
Reverse (-)
7177115 .. 7179265
2151 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G093700.1

Sequence Viewer

Length: 1578 bp
ATGGAGAAATCAACAATTGCTGCTATACTGCTTCTGCATCTCTCTATCTTTCAGTCTCAATCAGCGGTTCATTCCTTGGCTACTCCATCTGATAATGATTTTAGCTACATGGAATTTGTGTTCAATGCCACGGATCTACCGTTGGAAGAGGAATATGACTACATTATCGTAGGAGGAGGAACAGCAGGCTGCCCATTGGCAGCAACATTATCAGCAAACTACTCAGTCCTTGTTGTTGAAAGGGGCAGCATTCTGATCGAATATCCAAGTGTTTTGCGTGAAGAAGGGTTTGTGACGAATCTCATGCAAGAAGATGATGGTAAGACAGCAGCTCAAAAGTTCACGTCCGACGATGGTGTTTCTAATGCAAGAGGCAGAGTCTTAGGTGGGTCAAGCATGATCAATGCCGGTTTCTACTCGAGAGCTGAAAAGGAATTCTATGCGCAATCAGGAATTGAATGGGACATGGCTTTGGTTGATAAGGCCTATAGTTGGGTGGAAGACACTATTGTGTTCCAGCCGAAATTGGAAACTTGGCAATCTGTTGTAAAAGAAGCATTGTTGGAGGCTGGCGTGGGTCCAGACAATGGATTTAGTTTGGATCACATCAAGGGAACTAAAGTTGGGGGATCAATTTTCGATGATCATGGAAGGAGACATGGAGCTGTGGAACTGCTTAATAGAGGAGAGCCAAAGAACTTGCGAGTTGCAGTTCATGCCACAGTAGAGAGTATCATCTTCTCTTCCAATTCAATAAGTTTGTCAGCTATAGGAATCATATATAGTGATTCTAACGGGAAGACTCATTGGGCAAATGTACATAATAAGGGGGAGGTTATATTGAGTGCTGGAGCAATCGGAAGCCCTCAACTTCTGCTACTTAGTGGTATCTCAGTTATCCATCCTCAACCGAATATTGGACAGTTTATGTATGATAATCCTCGGAATTTCATTATCATTTTGCCCCCATCTCCACTGGATCTGTCAAGTCTACAGATAGTTGGAATTACAAGTGATTTCTACATAGAGACACTTTCTTATCACGTACCAGCTAACCCCAAATACTTGATTTTGGCAAACATTGCTGCAAAAGTACCCGGACCTTTGTCTTATGGTTCCCTTCGCCTGCAATCATCTTTTGACGTGAAAATTATTCCAAATGTCAGCTTCAACTATTTTACACATCCGATGGACCTTGCTCGTTGTTTTAGTGGCATGAGGAAGCTTCGTGATTTATTGAAGACAGATTCATTAGAACCATTCAAGACTAATTCTTCGTCTGGTACAGAAGGTTTCAAGTTTTATGGACCATCTTTACCAGTAAATCAGACAGATAATGCATCATTTGAAACATTTTGTCGAAGCACCGTAGCAACATTTTGGCATTACCATGGCGGATGCTTGGTGGGAAAGGTGGTTGATGGACGTTTGCGGGTCATGGGGATCAATGCATTACCTGTTGTTGATGGATCCACATTCAATTTCTCACCAGGAACCAATCCTCAGGCTACTCTAATGATGTTAGGCAGGTATGTTGGCCTTGAGGTACTGCAAGAAGGAGAAGTTTGCGAGGGATGA

Protein Analysis

526

Amino Acids

57.12

Weight (kDa)

5.22

Isoelectric Point (pI)

42.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 444
Acc36I ACCTGC 1 cut(s) 1518
AccB7I CCANNNNNTGG 1 cut(s) 587
AccI GTMKAC 1 cut(s) 991
AciI CCGC 3 cut(s) 65, 1395, 1432
AclWI GGATC 7 cut(s) 141, 609, 637, 987, 1451, 1464, 1477
AcsI RAATTY 3 cut(s) 113, 434, 946
AfaI GTAC 5 cut(s) 819, 1047, 1095, 1285, 1548
AfiI CCNNNNNNNGG 3 cut(s) 492, 587, 917
AjiI CACGTC 2 cut(s) 345, 1144
AjnI CCWGG 1 cut(s) 1489
AleI CACNNNNGTG 1 cut(s) 509
AloI GAACNNNNNNTCC 2 cut(s) 104, 136
AluBI AGCT 8 cut(s) 105, 332, 425, 665, 767, 1052, 1167, 1225
AluI AGCT 8 cut(s) 105, 332, 425, 665, 767, 1052, 1167, 1225
Alw26I GTCTC 3 cut(s) 60, 649, 1022
AlwI GGATC 7 cut(s) 141, 609, 637, 987, 1451, 1464, 1477
Ama87I CYCGRG 1 cut(s) 418
AoxI GGCC 2 cut(s) 483, 1537
ApeKI GCWGC 6 cut(s) 20, 189, 200, 246, 329, 1085
ApoI RAATTY 3 cut(s) 113, 434, 946
ArsI GACNNNNNNTTYG 2 cut(s) 1258, 1290
AspLEI GCGC 1 cut(s) 445
AspS9I GGNCC 4 cut(s) 578, 1100, 1192, 1307
AsuC2I CCSGG 1 cut(s) 1098
AsuHPI GGTGA 1 cut(s) 1479
AvaI CYCGRG 1 cut(s) 418
AvaII GGWCC 4 cut(s) 578, 1100, 1192, 1307
AxyI CCTNAGG 1 cut(s) 1503
BamHI GGATCC 1 cut(s) 1469
BbsI GAAGAC 3 cut(s) 507, 806, 1247
BbvI GCAGC 6 cut(s) 7, 176, 212, 258, 341, 1072
BccI CCATC 9 cut(s) 94, 311, 347, 909, 976, 1183, 1318, 1415, 1460
BcgI CGANNNNNNTGC 4 cut(s) 238, 272, 286, 320
BciT130I CCWGG 1 cut(s) 1491
BclI TGATCA 2 cut(s) 399, 643
BcnI CCSGG 1 cut(s) 1098
BcoDI GTCTC 3 cut(s) 60, 649, 1022
BfmI CTRYAG 3 cut(s) 487, 768, 992
BfuAI ACCTGC 1 cut(s) 1518
BisI GCNGC 6 cut(s) 21, 190, 201, 247, 330, 1086
BlsI GCNGC 6 cut(s) 22, 191, 202, 248, 331, 1087
Bme1390I CCNGG 2 cut(s) 1098, 1491
Bme18I GGWCC 4 cut(s) 578, 1100, 1192, 1307
BmeT110I CYCGRG 1 cut(s) 418
BmgBI CACGTC 2 cut(s) 345, 1144
BmgT120I GGNCC 4 cut(s) 578, 1100, 1192, 1307
BmiI GGNNCC 4 cut(s) 579, 1117, 1471, 1495
BmrFI CCNGG 2 cut(s) 1098, 1491
BmsI GCATC 3 cut(s) 46, 1349, 1388
BoxI GACNNNNGTC 1 cut(s) 1105
BpiI GAAGAC 3 cut(s) 507, 806, 1247
BpmI CTGGAG 1 cut(s) 870
BpuEI CTTGAG 1 cut(s) 1562
BpuMI CCSGG 1 cut(s) 1098
BsaAI YACGTR 1 cut(s) 1045
BsaJI CCNNGG 4 cut(s) 75, 129, 941, 1390
Bsc4I CCNNNNNNNGG 3 cut(s) 492, 587, 917
Bse118I RCCGGY 1 cut(s) 407
Bse1I ACTGG 2 cut(s) 981, 1319
Bse21I CCTNAGG 1 cut(s) 1503
Bse3DI GCAATG 1 cut(s) 1080
BseBI CCWGG 1 cut(s) 1491
BseDI CCNNGG 4 cut(s) 75, 129, 941, 1390
BseGI GGATG 3 cut(s) 901, 1183, 1403
BseLI CCNNNNNNNGG 3 cut(s) 492, 587, 917
BseMI GCAATG 1 cut(s) 1080
BseMII CTCAG 3 cut(s) 237, 906, 1517
BseNI ACTGG 2 cut(s) 981, 1319
BseRI GAGGAG 2 cut(s) 189, 699
BseXI GCAGC 6 cut(s) 7, 176, 212, 258, 341, 1072
BshFI GGCC 2 cut(s) 485, 1539
BsiHKCI CYCGRG 1 cut(s) 418
BsiSI CCGG 2 cut(s) 408, 1098
BslFI GGGAC 1 cut(s) 476
BslI CCNNNNNNNGG 3 cut(s) 492, 587, 917
BsmAI GTCTC 3 cut(s) 60, 649, 1022
BsmFI GGGAC 1 cut(s) 476
BsmI GAATGC 1 cut(s) 249
BsnI GGCC 2 cut(s) 485, 1539
BsoBI CYCGRG 1 cut(s) 418
Bsp1407I TGTACA 1 cut(s) 817
Bsp143I GATC 9 cut(s) 133, 255, 399, 601, 629, 643, 979, 1443, 1469
Bsp19I CCATGG 1 cut(s) 1390
BspACI CCGC 3 cut(s) 65, 1395, 1432
BspANI GGCC 2 cut(s) 485, 1539
BspCNI CTCAG 3 cut(s) 236, 905, 1516
BspLI GGNNCC 4 cut(s) 579, 1117, 1471, 1495
BspMI ACCTGC 1 cut(s) 1518
BspPI GGATC 7 cut(s) 141, 609, 637, 987, 1451, 1464, 1477
BsrDI GCAATG 1 cut(s) 1080
BsrFI RCCGGY 1 cut(s) 407
BsrGI TGTACA 1 cut(s) 817
BsrI ACTGG 2 cut(s) 981, 1319
BssAI RCCGGY 1 cut(s) 407
BssECI CCNNGG 4 cut(s) 75, 129, 941, 1390
BssMI GATC 9 cut(s) 133, 255, 399, 601, 629, 643, 979, 1443, 1469
BssT1I CCWWGG 2 cut(s) 75, 1390
Bst2UI CCWGG 1 cut(s) 1491
Bst4CI ACNGT 4 cut(s) 141, 724, 924, 1369
Bst6I CTCTTC 2 cut(s) 141, 748
BstAPI GCANNNNNTGC 2 cut(s) 716, 1082
BstAUI TGTACA 1 cut(s) 817
BstBAI YACGTR 1 cut(s) 1045
BstC8I GCNNGC 3 cut(s) 187, 571, 1127
BstDEI CTNAG 5 cut(s) 223, 382, 881, 892, 1503
BstDSI CCRYGG 2 cut(s) 129, 1390
BstF5I GGATG 3 cut(s) 901, 1183, 1403
BstHHI GCGC 1 cut(s) 445
BstKTI GATC 9 cut(s) 136, 258, 402, 604, 632, 646, 982, 1446, 1472
BstMAI GTCTC 3 cut(s) 60, 649, 1022
BstMBI GATC 9 cut(s) 133, 255, 399, 601, 629, 643, 979, 1443, 1469
BstMWI GCNNNNNNNGC 2 cut(s) 716, 1082
BstNI CCWGG 1 cut(s) 1491
BstPAI GACNNNNGTC 1 cut(s) 1105
BstSCI CCNGG 2 cut(s) 1096, 1489
BstSFI CTRYAG 3 cut(s) 487, 768, 992
BstV1I GCAGC 6 cut(s) 7, 176, 212, 258, 341, 1072
BstV2I GAAGAC 3 cut(s) 507, 806, 1247
BstX2I RGATCY 3 cut(s) 133, 979, 1469
BstYI RGATCY 3 cut(s) 133, 979, 1469
Bsu36I CCTNAGG 1 cut(s) 1503
BsuRI GGCC 2 cut(s) 485, 1539
BtgI CCRYGG 2 cut(s) 129, 1390
BtrI CACGTC 2 cut(s) 345, 1144
BtsCI GGATG 3 cut(s) 901, 1183, 1403
BtsIMutI CAGTG 1 cut(s) 974
BveI ACCTGC 1 cut(s) 1518
Cac8I GCNNGC 3 cut(s) 187, 571, 1127
CfoI GCGC 1 cut(s) 445
Cfr10I RCCGGY 1 cut(s) 407
Cfr13I GGNCC 4 cut(s) 578, 1100, 1192, 1307
Csp6I GTAC 5 cut(s) 818, 1046, 1094, 1284, 1547
CviQI GTAC 5 cut(s) 818, 1046, 1094, 1284, 1547
DdeI CTNAG 5 cut(s) 223, 382, 881, 892, 1503
DpnI GATC 9 cut(s) 135, 257, 401, 603, 631, 645, 981, 1445, 1471
DpnII GATC 9 cut(s) 133, 255, 399, 601, 629, 643, 979, 1443, 1469
Eam1104I CTCTTC 2 cut(s) 141, 748
EarI CTCTTC 2 cut(s) 141, 748
EciI GGCGGA 1 cut(s) 1410
Eco130I CCWWGG 2 cut(s) 75, 1390
Eco147I AGGCCT 1 cut(s) 485
Eco47I GGWCC 4 cut(s) 578, 1100, 1192, 1307
Eco81I CCTNAGG 1 cut(s) 1503
Eco88I CYCGRG 1 cut(s) 418
EcoRI GAATTC 1 cut(s) 434
EcoRII CCWGG 1 cut(s) 1489
EcoT14I CCWWGG 2 cut(s) 75, 1390
EcoT22I ATGCAT 2 cut(s) 1342, 1453
ErhI CCWWGG 2 cut(s) 75, 1390
FaqI GGGAC 1 cut(s) 476
FauI CCCGC 1 cut(s) 1425
FbaI TGATCA 2 cut(s) 399, 643
FblI GTMKAC 1 cut(s) 991
Fnu4HI GCNGC 6 cut(s) 21, 190, 201, 247, 330, 1086
FokI GGATG 3 cut(s) 888, 1170, 1410
Fsp4HI GCNGC 6 cut(s) 21, 190, 201, 247, 330, 1086
FspI TGCGCA 1 cut(s) 444
GlaI GCGC 1 cut(s) 444
GluI GCNGC 6 cut(s) 21, 190, 201, 247, 330, 1086
GsuI CTGGAG 1 cut(s) 870
HaeIII GGCC 2 cut(s) 485, 1539
HapII CCGG 2 cut(s) 408, 1098
HhaI GCGC 1 cut(s) 445
Hin6I GCGC 1 cut(s) 443
HinP1I GCGC 1 cut(s) 443
HindIII AAGCTT 1 cut(s) 1223
HinfI GANTC 6 cut(s) 298, 378, 774, 788, 802, 1247
HpaII CCGG 2 cut(s) 408, 1098
HphI GGTGA 1 cut(s) 1479
Hpy166II GTNNAC 2 cut(s) 342, 992
Hpy188I TCNGA 7 cut(s) 91, 255, 349, 860, 945, 1188, 1329
Hpy188III TCNNGA 5 cut(s) 420, 450, 581, 1229, 1264
Hpy8I GTNNAC 2 cut(s) 342, 992
Hpy99I CGWCG 1 cut(s) 353
HpyAV CCTTC 5 cut(s) 278, 645, 1130, 1283, 1550
HpyCH4III ACNGT 4 cut(s) 141, 724, 924, 1369
HpyCH4IV ACGT 4 cut(s) 344, 1044, 1143, 1426
HpyCH4V TGCA 9 cut(s) 37, 307, 368, 710, 1088, 1129, 1340, 1451, 1552
HpyF10VI GCNNNNNNNGC 2 cut(s) 716, 1082
HpyF3I CTNAG 5 cut(s) 223, 382, 881, 892, 1503
HpySE526I ACGT 4 cut(s) 344, 1044, 1143, 1426
HspAI GCGC 1 cut(s) 443
Ksp22I TGATCA 2 cut(s) 399, 643
Kzo9I GATC 9 cut(s) 133, 255, 399, 601, 629, 643, 979, 1443, 1469
LmnI GCTCC 2 cut(s) 662, 851
Lsp1109I GCAGC 6 cut(s) 7, 176, 212, 258, 341, 1072
LweI GCATC 3 cut(s) 46, 1349, 1388
MaeII ACGT 4 cut(s) 344, 1044, 1143, 1426
MaeIII GTNAC 1 cut(s) 292
MalI GATC 9 cut(s) 135, 257, 401, 603, 631, 645, 981, 1445, 1471
MboI GATC 9 cut(s) 133, 255, 399, 601, 629, 643, 979, 1443, 1469
MboII GAAGA 9 cut(s) 158, 293, 323, 512, 730, 735, 811, 1252, 1266
MfeI CAATTG 1 cut(s) 15
MflI RGATCY 3 cut(s) 133, 979, 1469
MlyI GAGTC 2 cut(s) 387, 796
MmeI TCCRAC 4 cut(s) 123, 372, 543, 982
Mph1103I ATGCAT 2 cut(s) 1342, 1453
MseI TTAA 1 cut(s) 678
MslI CAYNNNNRTG 2 cut(s) 509, 1389
MspA1I CMGCKG 1 cut(s) 65
MspI CCGG 2 cut(s) 408, 1098
MspR9I CCNGG 2 cut(s) 1098, 1491
MunI CAATTG 1 cut(s) 15
Mva1269I GAATGC 1 cut(s) 249
MvaI CCWGG 1 cut(s) 1491
MwoI GCNNNNNNNGC 2 cut(s) 716, 1082
NciI CCSGG 1 cut(s) 1098
NcoI CCATGG 1 cut(s) 1390
NdeII GATC 9 cut(s) 133, 255, 399, 601, 629, 643, 979, 1443, 1469
NlaIV GGNNCC 4 cut(s) 579, 1117, 1471, 1495
NmuCI GTSAC 1 cut(s) 292
NsbI TGCGCA 1 cut(s) 444
NsiI ATGCAT 2 cut(s) 1342, 1453
OliI CACNNNNGTG 1 cut(s) 509
PaeR7I CTCGAG 1 cut(s) 418
PceI AGGCCT 1 cut(s) 485
PcsI WCGNNNNNNNCGW 1 cut(s) 137
PctI GAATGC 1 cut(s) 249
PfeI GAWTC 4 cut(s) 298, 774, 788, 1247
PflMI CCANNNNNTGG 1 cut(s) 587
PkrI GCNGC 6 cut(s) 22, 191, 202, 248, 331, 1087
PleI GAGTC 2 cut(s) 386, 796
PpsI GAGTC 2 cut(s) 386, 796
Ppu21I YACGTR 1 cut(s) 1045
PshAI GACNNNNGTC 1 cut(s) 1105
Psp6I CCWGG 1 cut(s) 1489
PspGI CCWGG 1 cut(s) 1489
PspN4I GGNNCC 4 cut(s) 579, 1117, 1471, 1495
PspPI GGNCC 4 cut(s) 578, 1100, 1192, 1307
PsuI RGATCY 3 cut(s) 133, 979, 1469
RsaI GTAC 5 cut(s) 819, 1047, 1095, 1285, 1548
RsaNI GTAC 5 cut(s) 818, 1046, 1094, 1284, 1547
RseI CAYNNNNRTG 2 cut(s) 509, 1389
SaqAI TTAA 1 cut(s) 678
SatI GCNGC 6 cut(s) 21, 190, 201, 247, 330, 1086
Sau3AI GATC 9 cut(s) 133, 255, 399, 601, 629, 643, 979, 1443, 1469
Sau96I GGNCC 4 cut(s) 578, 1100, 1192, 1307
SchI GAGTC 2 cut(s) 387, 796
ScrFI CCNGG 2 cut(s) 1098, 1491
SfaNI GCATC 3 cut(s) 46, 1349, 1388
SfcI CTRYAG 3 cut(s) 487, 768, 992
Sfr274I CTCGAG 1 cut(s) 418
SinI GGWCC 4 cut(s) 578, 1100, 1192, 1307
SlaI CTCGAG 1 cut(s) 418
SmiMI CAYNNNNRTG 2 cut(s) 509, 1389
SmlI CTYRAG 2 cut(s) 418, 1541
SmoI CTYRAG 2 cut(s) 418, 1541
SseBI AGGCCT 1 cut(s) 485
SsiI CCGC 3 cut(s) 65, 1395, 1432
SspI AATATT 1 cut(s) 916
StuI AGGCCT 1 cut(s) 485
StyD4I CCNGG 2 cut(s) 1096, 1489
StyI CCWWGG 2 cut(s) 75, 1390
TaaI ACNGT 4 cut(s) 141, 724, 924, 1369
TaiI ACGT 4 cut(s) 347, 1047, 1146, 1429
TaqI TCGA 4 cut(s) 258, 419, 639, 1360
TatI WGTACW 1 cut(s) 817
TfiI GAWTC 4 cut(s) 298, 774, 788, 1247
Tru1I TTAA 1 cut(s) 678
Tru9I TTAA 1 cut(s) 678
TscAI CASTG 1 cut(s) 981
TseFI GTSAC 1 cut(s) 292
TseI GCWGC 6 cut(s) 20, 189, 200, 246, 329, 1085
Tsp45I GTSAC 1 cut(s) 292
TspDTI ATGAA 4 cut(s) 59, 704, 940, 1239
TspGWI ACGGA 1 cut(s) 146
TspRI CASTG 1 cut(s) 981
Van91I CCANNNNNTGG 1 cut(s) 587
VpaK11BI GGWCC 4 cut(s) 578, 1100, 1192, 1307
XapI RAATTY 3 cut(s) 113, 434, 946
XhoI CTCGAG 1 cut(s) 418
XmiI GTMKAC 1 cut(s) 991
Zsp2I ATGCAT 2 cut(s) 1342, 1453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.