MD03G1091700.v1.1

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000533
Physical Location & Seq
Forward (+)
7624691 .. 7626803
2113 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1091700.v1.1.491

Sequence Viewer

Length: 1263 bp
ATGGAGAAATCAACAACGGCTGCTATATTATTGCTCTTGTACTTTTTTGAGTTCTGCCCTCAACCAGGGGTTTCCTCATTCGCCAATCCATCTGATGACCTTGATTTTAGCTACTTGAAATTTGTATGTAACGCAACTGATCTACCATTACAAGAAGAATATGACTACATTGTTGTGGGAGTGGGCACGGCAGGGTGCCCGTTGGTAACAACTTTATCTGCAAACTACTCCGTTCTCCTTCTAGAAAGGGGCAATATTCCATCAGCATATCCAAATGTGTTGTGTCAAAATGAGACTCTTGCAAATTTCATGCAAGAAGATGACGGTAAGACGCCGGTCCAGAGGTTCACATCAGAAGATGGTGTAGCTAATGTAAGAGGACGGATCCTAGGCTGGTCAAGTATGATCAATATTGGTATATACTCAAGAGCCGACAGTGAATTCTACGAGAAATCAGGAATTAAATTGGACATGAACTTAGTGAATAATTCATATGAATGGGTTGAAAACACTGTAATATTCCGTCCGAATGTAACACGCTGGCAATCTGTTGTGAAAGATGCGATGTTAGAAGCTGGTGTTCGTCCAGACAATGGACTGACTTTGGATCACATTCTGGGAAGTAAAGTCACGGGTACGCTCTTAGACAATCGTGGAAAAAGACATGGAGCTGTGGAACACCTAAATAAAGGACATCCAAAAAACCTGAGAGTTGCAATTCATGCCACAGTAGAGAGGATCATTTTCTCTTCCAAAGCATCAGGTTTGTCAGCTAAAGGAATCATATACAGTGATTCTAACGGGAGGTCTCATCGGGCATTGATACGTGGTAAGGGTGAGGTTATATTGAGTGCAGGTGAAATTGGAAGTCCTCAACTTCTACTACTTAGTGGTGTTGGCCCAAAATCTTACCTTTCATCTCGTAAAATCCCAGTTGTTCACCCACAACCTTACGTTGGGCAGTTTATGCGTGATAATCCTCGTAATTACATTACCACTTTGGCCCCGTTTCAAGTAGAACCTTCTACTGCACAGTTTGTTGGTATCACAAGTGATTATTACATAGAGACTTTCTCCGGCTTGCCATTTTCTACTCAGGCCTTTAGTCTTTTCCCTAGTCCAACAATTCCCATGACCATAAATTCAAGTTTCGGGCACATTATTTTCAAATTTCCAGGACCCTTGTCCTATGGTTCTCTTAAGCTGCAATCATCCTATGATGTCAAAGTTGCTCCAAATGTCAAATTCAACTACTTTGCATAG

Protein Analysis

421

Amino Acids

46.24

Weight (kDa)

7.09

Isoelectric Point (pI)

36.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_N PF00732 54 - 321 9.7e-17 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 845
Acc36I ACCTGC 1 cut(s) 845
AccB1I GGYRCC 1 cut(s) 195
AccB7I CCANNNNNTGG 1 cut(s) 593
AclWI GGATC 4 cut(s) 379, 392, 615, 746
AcsI RAATTY 6 cut(s) 119, 304, 440, 1141, 1169, 1244
AcyI GRCGYC 1 cut(s) 332
AfaI GTAC 2 cut(s) 41, 637
AfiI CCNNNNNNNGG 3 cut(s) 65, 593, 956
AflII CTTAAG 1 cut(s) 1199
AgsI TTSAA 6 cut(s) 118, 506, 1013, 1146, 1168, 1249
AjnI CCWGG 2 cut(s) 64, 1174
AluBI AGCT 6 cut(s) 111, 368, 575, 671, 773, 1204
AluI AGCT 6 cut(s) 111, 368, 575, 671, 773, 1204
Alw26I GTCTC 3 cut(s) 287, 813, 1061
AlwI GGATC 4 cut(s) 379, 392, 615, 746
AoxI GGCC 3 cut(s) 898, 1002, 1098
ApeKI GCWGC 2 cut(s) 20, 1204
ApoI RAATTY 6 cut(s) 119, 304, 440, 1141, 1169, 1244
AspA2I CCTAGG 1 cut(s) 388
AspS9I GGNCC 4 cut(s) 337, 899, 1003, 1178
AsuHPI GGTGA 3 cut(s) 848, 869, 932
AvaII GGWCC 2 cut(s) 337, 1178
AvrII CCTAGG 1 cut(s) 388
BaeGI GKGCMC 3 cut(s) 188, 200, 1158
BamHI GGATCC 1 cut(s) 384
BanI GGYRCC 1 cut(s) 195
BbvI GCAGC 2 cut(s) 7, 1191
BccI CCATC 3 cut(s) 97, 268, 353
BceAI ACGGC 2 cut(s) 33, 204
BciT130I CCWGG 2 cut(s) 66, 1176
BclI TGATCA 1 cut(s) 405
BcoDI GTCTC 3 cut(s) 287, 813, 1061
BfaI CTAG 3 cut(s) 242, 389, 1116
BfrI CTTAAG 1 cut(s) 1199
BfuAI ACCTGC 1 cut(s) 845
BisI GCNGC 2 cut(s) 21, 1205
BlnI CCTAGG 1 cut(s) 388
BlsI GCNGC 2 cut(s) 22, 1206
Bme1390I CCNGG 2 cut(s) 66, 1176
Bme18I GGWCC 2 cut(s) 337, 1178
BmgT120I GGNCC 4 cut(s) 337, 899, 1003, 1178
BmiI GGNNCC 4 cut(s) 197, 386, 1005, 1180
BmrFI CCNGG 2 cut(s) 66, 1176
BmrI ACTGGG 1 cut(s) 926
BmsI GCATC 2 cut(s) 550, 767
BmuI ACTGGG 1 cut(s) 926
BoxI GACNNNNGTC 2 cut(s) 335, 1183
BplI GAGNNNNNCTC 2 cut(s) 1058, 1090
BpuEI CTTGAG 1 cut(s) 409
BsaAI YACGTR 1 cut(s) 827
BsaHI GRCGYC 1 cut(s) 332
BsaI GGTCTC 1 cut(s) 813
BsaJI CCNNGG 2 cut(s) 65, 388
Bsc4I CCNNNNNNNGG 3 cut(s) 65, 593, 956
Bse118I RCCGGY 1 cut(s) 334
Bse1I ACTGG 1 cut(s) 932
BseBI CCWGG 2 cut(s) 66, 1176
BseDI CCNNGG 2 cut(s) 65, 388
BseGI GGATG 2 cut(s) 694, 1211
BseLI CCNNNNNNNGG 3 cut(s) 65, 593, 956
BseMII CTCAG 2 cut(s) 698, 1109
BseNI ACTGG 1 cut(s) 932
BseSI GKGCMC 3 cut(s) 188, 200, 1158
BseXI GCAGC 2 cut(s) 7, 1191
BsgI GTGCAG 2 cut(s) 873, 1014
BshFI GGCC 3 cut(s) 900, 1004, 1100
BshNI GGYRCC 1 cut(s) 195
BsiSI CCGG 2 cut(s) 335, 1077
BslI CCNNNNNNNGG 3 cut(s) 65, 593, 956
BsmAI GTCTC 3 cut(s) 287, 813, 1061
BsnI GGCC 3 cut(s) 900, 1004, 1100
Bso31I GGTCTC 1 cut(s) 813
Bsp1286I GDGCHC 3 cut(s) 188, 200, 1158
Bsp143I GATC 5 cut(s) 139, 384, 405, 607, 738
BspANI GGCC 3 cut(s) 900, 1004, 1100
BspCNI CTCAG 2 cut(s) 699, 1108
BspLI GGNNCC 4 cut(s) 197, 386, 1005, 1180
BspMI ACCTGC 1 cut(s) 845
BspPI GGATC 4 cut(s) 379, 392, 615, 746
BspT107I GGYRCC 1 cut(s) 195
BspTI CTTAAG 1 cut(s) 1199
BspTNI GGTCTC 1 cut(s) 813
BsrFI RCCGGY 1 cut(s) 334
BsrI ACTGG 1 cut(s) 932
BssAI RCCGGY 1 cut(s) 334
BssECI CCNNGG 2 cut(s) 65, 388
BssMI GATC 5 cut(s) 139, 384, 405, 607, 738
BssNI GRCGYC 1 cut(s) 332
BssT1I CCWWGG 1 cut(s) 388
Bst2UI CCWGG 2 cut(s) 66, 1176
Bst4CI ACNGT 6 cut(s) 326, 437, 514, 730, 791, 1035
Bst6I CTCTTC 1 cut(s) 754
BstACI GRCGYC 1 cut(s) 332
BstAFI CTTAAG 1 cut(s) 1199
BstAPI GCANNNNNTGC 2 cut(s) 722, 967
BstBAI YACGTR 1 cut(s) 827
BstC8I GCNNGC 2 cut(s) 542, 1082
BstDEI CTNAG 5 cut(s) 478, 643, 707, 887, 1095
BstENI CCTNNNNNAGG 1 cut(s) 63
BstF5I GGATG 2 cut(s) 694, 1211
BstKTI GATC 5 cut(s) 142, 387, 408, 610, 741
BstMAI GTCTC 3 cut(s) 287, 813, 1061
BstMBI GATC 5 cut(s) 139, 384, 405, 607, 738
BstMWI GCNNNNNNNGC 2 cut(s) 722, 967
BstNI CCWGG 2 cut(s) 66, 1176
BstPAI GACNNNNGTC 2 cut(s) 335, 1183
BstSCI CCNGG 2 cut(s) 64, 1174
BstSLI GKGCMC 3 cut(s) 188, 200, 1158
BstV1I GCAGC 2 cut(s) 7, 1191
BstX2I RGATCY 1 cut(s) 384
BstYI RGATCY 1 cut(s) 384
BsuRI GGCC 3 cut(s) 900, 1004, 1100
BtgZI GCGATG 1 cut(s) 578
BtsCI GGATG 2 cut(s) 694, 1211
BtsIMutI CAGTG 3 cut(s) 442, 510, 796
BveI ACCTGC 1 cut(s) 845
Cac8I GCNNGC 2 cut(s) 542, 1082
Cfr10I RCCGGY 1 cut(s) 334
Cfr13I GGNCC 4 cut(s) 337, 899, 1003, 1178
CseI GACGC 1 cut(s) 340
Csp6I GTAC 2 cut(s) 40, 636
CviAII CATG 5 cut(s) 310, 472, 665, 722, 1132
CviQI GTAC 2 cut(s) 40, 636
DdeI CTNAG 5 cut(s) 478, 643, 707, 887, 1095
DpnI GATC 5 cut(s) 141, 386, 407, 609, 740
DpnII GATC 5 cut(s) 139, 384, 405, 607, 738
Eam1104I CTCTTC 1 cut(s) 754
EarI CTCTTC 1 cut(s) 754
Eco130I CCWWGG 1 cut(s) 388
Eco147I AGGCCT 1 cut(s) 1100
Eco31I GGTCTC 1 cut(s) 813
Eco47I GGWCC 2 cut(s) 337, 1178
EcoNI CCTNNNNNAGG 1 cut(s) 63
EcoO109I RGGNCCY 1 cut(s) 1178
EcoRI GAATTC 1 cut(s) 440
EcoRII CCWGG 2 cut(s) 64, 1174
EcoT14I CCWWGG 1 cut(s) 388
ErhI CCWWGG 1 cut(s) 388
FaeI CATG 5 cut(s) 313, 475, 668, 725, 1135
FatI CATG 5 cut(s) 309, 471, 664, 721, 1131
FauNDI CATATG 1 cut(s) 493
FbaI TGATCA 1 cut(s) 405
Fnu4HI GCNGC 2 cut(s) 21, 1205
FokI GGATG 2 cut(s) 681, 1198
Fsp4HI GCNGC 2 cut(s) 21, 1205
FspBI CTAG 3 cut(s) 242, 389, 1116
GluI GCNGC 2 cut(s) 21, 1205
HaeIII GGCC 3 cut(s) 900, 1004, 1100
HapII CCGG 2 cut(s) 335, 1077
HgaI GACGC 1 cut(s) 340
Hin1I GRCGYC 1 cut(s) 332
Hin1II CATG 5 cut(s) 313, 475, 668, 725, 1135
HinfI GANTC 3 cut(s) 295, 780, 794
HpaII CCGG 2 cut(s) 335, 1077
HphI GGTGA 3 cut(s) 848, 869, 932
Hpy166II GTNNAC 2 cut(s) 348, 940
Hpy188I TCNGA 3 cut(s) 94, 355, 528
Hpy188III TCNNGA 5 cut(s) 242, 340, 426, 456, 587
Hpy8I GTNNAC 2 cut(s) 348, 940
HpyAV CCTTC 2 cut(s) 248, 1032
HpyCH4III ACNGT 6 cut(s) 326, 437, 514, 730, 791, 1035
HpyCH4IV ACGT 2 cut(s) 826, 954
HpyCH4V TGCA 8 cut(s) 221, 302, 313, 716, 854, 1031, 1207, 1259
HpyF10VI GCNNNNNNNGC 2 cut(s) 722, 967
HpyF3I CTNAG 5 cut(s) 478, 643, 707, 887, 1095
HpySE526I ACGT 2 cut(s) 826, 954
Hsp92I GRCGYC 1 cut(s) 332
Hsp92II CATG 5 cut(s) 313, 475, 668, 725, 1135
Ksp22I TGATCA 1 cut(s) 405
Kzo9I GATC 5 cut(s) 139, 384, 405, 607, 738
LmnI GCTCC 2 cut(s) 668, 1237
Lsp1109I GCAGC 2 cut(s) 7, 1191
LweI GCATC 2 cut(s) 550, 767
MaeI CTAG 3 cut(s) 242, 389, 1116
MaeII ACGT 2 cut(s) 826, 954
MaeIII GTNAC 4 cut(s) 128, 205, 532, 628
MalI GATC 5 cut(s) 141, 386, 407, 609, 740
MboI GATC 5 cut(s) 139, 384, 405, 607, 738
MboII GAAGA 4 cut(s) 167, 329, 368, 741
MflI RGATCY 1 cut(s) 384
MhlI GDGCHC 3 cut(s) 188, 200, 1158
MlyI GAGTC 1 cut(s) 289
MmeI TCCRAC 1 cut(s) 1145
MnlI CCTC 9 cut(s) 69, 85, 336, 371, 729, 798, 832, 882, 990
MseI TTAA 2 cut(s) 462, 1200
MslI CAYNNNNRTG 2 cut(s) 173, 496
MspCI CTTAAG 1 cut(s) 1199
MspI CCGG 2 cut(s) 335, 1077
MspR9I CCNGG 2 cut(s) 66, 1176
MvaI CCWGG 2 cut(s) 66, 1176
MwoI GCNNNNNNNGC 2 cut(s) 722, 967
NdeI CATATG 1 cut(s) 493
NdeII GATC 5 cut(s) 139, 384, 405, 607, 738
NlaIII CATG 5 cut(s) 313, 475, 668, 725, 1135
NlaIV GGNNCC 4 cut(s) 197, 386, 1005, 1180
NmuCI GTSAC 1 cut(s) 628
PaqCI CACCTGC 1 cut(s) 845
PceI AGGCCT 1 cut(s) 1100
PfeI GAWTC 2 cut(s) 780, 794
PflMI CCANNNNNTGG 1 cut(s) 593
PfoI TCCNGGA 1 cut(s) 1174
PkrI GCNGC 2 cut(s) 22, 1206
PleI GAGTC 1 cut(s) 289
PpsI GAGTC 1 cut(s) 289
Ppu21I YACGTR 1 cut(s) 827
PpuMI RGGWCCY 1 cut(s) 1178
PshAI GACNNNNGTC 2 cut(s) 335, 1183
Psp5II RGGWCCY 1 cut(s) 1178
Psp6I CCWGG 2 cut(s) 64, 1174
PspGI CCWGG 2 cut(s) 64, 1174
PspN4I GGNNCC 4 cut(s) 197, 386, 1005, 1180
PspPI GGNCC 4 cut(s) 337, 899, 1003, 1178
PspPPI RGGWCCY 1 cut(s) 1178
PsuI RGATCY 1 cut(s) 384
RsaI GTAC 2 cut(s) 41, 637
RsaNI GTAC 2 cut(s) 40, 636
RseI CAYNNNNRTG 2 cut(s) 173, 496
SaqAI TTAA 2 cut(s) 462, 1200
SatI GCNGC 2 cut(s) 21, 1205
Sau3AI GATC 5 cut(s) 139, 384, 405, 607, 738
Sau96I GGNCC 4 cut(s) 337, 899, 1003, 1178
SchI GAGTC 1 cut(s) 289
ScrFI CCNGG 2 cut(s) 66, 1176
SduI GDGCHC 3 cut(s) 188, 200, 1158
SfaNI GCATC 2 cut(s) 550, 767
SinI GGWCC 2 cut(s) 337, 1178
SmiMI CAYNNNNRTG 2 cut(s) 173, 496
SmlI CTYRAG 2 cut(s) 424, 1199
SmoI CTYRAG 2 cut(s) 424, 1199
SseBI AGGCCT 1 cut(s) 1100
SspI AATATT 3 cut(s) 256, 412, 519
SspMI CTAG 3 cut(s) 242, 389, 1116
StuI AGGCCT 1 cut(s) 1100
StyD4I CCNGG 2 cut(s) 64, 1174
StyI CCWWGG 1 cut(s) 388
TaaI ACNGT 6 cut(s) 326, 437, 514, 730, 791, 1035
TaiI ACGT 2 cut(s) 829, 957
TatI WGTACW 1 cut(s) 39
TfiI GAWTC 2 cut(s) 780, 794
Tru1I TTAA 2 cut(s) 462, 1200
Tru9I TTAA 2 cut(s) 462, 1200
TscAI CASTG 3 cut(s) 442, 517, 796
TseFI GTSAC 1 cut(s) 628
TseI GCWGC 2 cut(s) 20, 1204
Tsp45I GTSAC 1 cut(s) 628
TspDTI ATGAA 6 cut(s) 298, 480, 488, 510, 710, 906
TspGWI ACGGA 3 cut(s) 220, 397, 512
TspRI CASTG 3 cut(s) 442, 517, 796
Van91I CCANNNNNTGG 1 cut(s) 593
Vha464I CTTAAG 1 cut(s) 1199
VpaK11BI GGWCC 2 cut(s) 337, 1178
XagI CCTNNNNNAGG 1 cut(s) 63
XapI RAATTY 6 cut(s) 119, 304, 440, 1141, 1169, 1244
XbaI TCTAGA 1 cut(s) 241
XmaJI CCTAGG 1 cut(s) 388
XspI CTAG 3 cut(s) 242, 389, 1116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.