MD16G1265300.v1.1

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000533
Physical Location & Seq
Forward (+)
33733068 .. 33737935
4868 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1265300.v1.1.491

Sequence Viewer

Length: 1968 bp
ATGATGCATACTGCGTCCAATTCTCGCCATTCAGTAGCATACGGTTTCAAGATTATTGTGAGTTCTTCAGAGAAGTTTGAGAGTTCTTCAGAGAAGTTTGAGCTTGTTTTCCTTGAGGATACAAGTGCTGCTTCCACACCAAGTCCATCTTTGTACCTGCAGGAAACAGAGACTGACAAACCGAGAATACGTTTGCCTTTCGGAATTCAGAAAATGGAGAAATCAACAATGTTTGTTGTACTATTGGTGTTGCAACTTTTTGTCCATCTTCAATTAGAGGTTCACTCGTTGGCCACTCTTTCTGAACATGATTTTAGCTACTCAAAATCTGTGATTAATGCCACTGATCTTCCACTAGAAGAAGTGTATGACTACATTGTAGTTGGAGGGGGTACCGCAGGGTGTCCATTGGCAGCAACTTTATCATCAAAGTATTCGGTGCTCGTTTTAGAAAGAGGAAGTTTTCCTACATCATATCCGAATGTCTTGACTCAAGAGGGGTTTATATATAATCTCCAGCAAGAAGATGATGGAGAGACACCAGTTCAAAGGGTCATGTCAGAAGATGGCATTCCTACTGTTAGAGGAAGGATCCTTGGCGGCACGAGCATGATCAGTGCTGGCGTATACGCAAGAGCTAACATTTCATTCTTTAATGAATCGGGAGTTGAATGGGACATCAATTTGGTTAATGCGACATATGAGTGGATTGAAGACACTATTGTGTACAAGCCAAATGCATTCGCTTGGCAAACTGTTACACAAAAAGCTTTTTTGGAGGCTGGCGTTTTACCAGAAAATGGATTCAGTTTGGATCACGTACCTGAAACAAGAATCACTGGCTCAACTTTTGACAATAGTGGAACGAGACATGCGGCTGATGAACTACTTAACAGAGGAGACCTAGACAACTTGCGAGTTGCAGTTCATGCCAACGTAGAGAAGATCGTCTTCTCTTCTAGTGAATCAAGATTGTCAGCTACAGGAGTCATATATAAGGATTCTAACGGAATTTCTCATCGGGCATATGTACGCGATCAAGGTGAAGTTATATTGAGTGCAGGGACAATGGGGACCCCTCAACTTCTACTACTCAGTGGCGTTGGCCCAGAGTCTTATCTATCATCTCTTGGAATACCAGTTGTGCTTGACCATCCTTACGTTGGACAGTTTCTATACGATAATCCTCGTAATTTCATTAACATTTTGCCCCCAAATCCAATAGAACCCTCAATTGTAACAGTTCTAGGCATTAGAGACGATTTCTGGCAATGTTCTATCTCGTGCGGACCACTTACTGCTCCACCCTATAGTTTATTTCCTAGTCAGTCTTATCCCCTGCCAATAAATTCAACTTTCGCACACATTCCTAACAAAGTGCCAGGACCTCATTCTCATGGTTCTCTCACGCTAAATTCATCTTCTGATGTGACAATTGGTCCAAATGTTAGATTCAATTACTTTTCAAATGCGGCGGACCTTGCTCATTGTGTTAGCGGCATGAAACAAATTGGTTATTTATTAAGGACTAATGCATTTAAACCATATAAAACCCAAGATTTACCAGGTATAGAAGGTTTCACCTTTTTGGGACTACCTCTGCCAAATAATCAAACAGATGATGCACTCGAAACATTTTGCCGAGATTCATTGGCCTCATATTGGCATTACCACGGTGGATGCATTGTTGGAAAGGTGGTTGATGGTGGTTTGCATGTTAATGGAATTGATGCATTACGCATAGTTGATTCTTCCACATTCCCTGTCACACCGGCGAGCCACCCTATGGGCTTCTATCTGATGTTAGGCAGGTATATGGGTCTTCAAATTCTGCAAGAGAGATCAGCTTGGGTAGGTATGTCATGCATGGACGGGGCCATGCAAAGCCTACAGCGGGGCATCTGCCCCCACCCAATGAATTTTGAAGAAAACCTTGAAGGGGTCAATTGTCAATCAACTCTAATATAA

Protein Analysis

656

Amino Acids

71.8

Weight (kDa)

5.1

Isoelectric Point (pI)

42.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_N PF00732 123 - 394 1.4e-16 GMC oxidoreductase
GMC_oxred_C PF05199 463 - 603 3.1e-23 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 165, 1800
Acc65I GGTACC 1 cut(s) 392
AccB1I GGYRCC 1 cut(s) 392
AccB7I CCANNNNNTGG 2 cut(s) 800, 1786
AccI GTMKAC 1 cut(s) 627
AccII CGCG 1 cut(s) 1035
AciI CCGC 8 cut(s) 396, 600, 875, 1287, 1472, 1475, 1497, 1894
AclWI GGATC 3 cut(s) 586, 599, 822
AcoI YGGCCR 1 cut(s) 291
AcsI RAATTY 6 cut(s) 204, 1011, 1348, 1414, 1827, 1918
AcuI CTGAAG 2 cut(s) 51, 72
AfaI GTAC 6 cut(s) 155, 240, 394, 728, 822, 1032
AfiI CCNNNNNNNGG 6 cut(s) 800, 1163, 1662, 1786, 1894, 1939
AhdI GACNNNNNGTC 1 cut(s) 1437
AjnI CCWGG 2 cut(s) 1381, 1564
AleI CACNNNNGTG 1 cut(s) 722
AluBI AGCT 6 cut(s) 103, 318, 638, 770, 980, 1847
AluI AGCT 6 cut(s) 103, 318, 638, 770, 980, 1847
Alw21I GWGCWC 1 cut(s) 444
Alw26I GTCTC 5 cut(s) 164, 530, 862, 894, 1251
AlwI GGATC 3 cut(s) 586, 599, 822
AlwNI CAGNNNCTG 1 cut(s) 173
AoxI GGCC 4 cut(s) 291, 1105, 1653, 1875
ApeKI GCWGC 2 cut(s) 128, 413
ApoI RAATTY 6 cut(s) 204, 1011, 1348, 1414, 1827, 1918
AseI ATTAAT 1 cut(s) 336
Asp718I GGTACC 1 cut(s) 392
AspS9I GGNCC 7 cut(s) 1074, 1106, 1289, 1385, 1439, 1477, 1875
AsuHPI GGTGA 2 cut(s) 1055, 1573
AvaII GGWCC 5 cut(s) 1074, 1289, 1385, 1439, 1477
BalI TGGCCA 1 cut(s) 293
BamHI GGATCC 1 cut(s) 591
BanI GGYRCC 1 cut(s) 392
BarI GAAGNNNNNNTAC 2 cut(s) 451, 483
BauI CACGAG 2 cut(s) 604, 1282
BbsI GAAGAC 3 cut(s) 720, 943, 1814
Bbv12I GWGCWC 1 cut(s) 444
BbvI GCAGC 2 cut(s) 115, 425
BccI CCATC 6 cut(s) 154, 273, 524, 560, 1161, 1697
BciT130I CCWGG 2 cut(s) 1383, 1566
BciVI GTATCC 1 cut(s) 112
BclI TGATCA 1 cut(s) 612
BcoDI GTCTC 5 cut(s) 164, 530, 862, 894, 1251
BfaI CTAG 5 cut(s) 356, 905, 960, 1247, 1323
BfmI CTRYAG 4 cut(s) 158, 981, 1309, 1889
BfuAI ACCTGC 2 cut(s) 165, 1800
BfuI GTATCC 1 cut(s) 112
BisI GCNGC 6 cut(s) 129, 414, 601, 876, 1473, 1498
BlsI GCNGC 6 cut(s) 130, 415, 602, 877, 1474, 1499
Bme1390I CCNGG 2 cut(s) 1383, 1566
Bme18I GGWCC 5 cut(s) 1074, 1289, 1385, 1439, 1477
BmeRI GACNNNNNGTC 1 cut(s) 1437
BmgT120I GGNCC 7 cut(s) 1074, 1106, 1289, 1385, 1439, 1477, 1875
BmiI GGNNCC 5 cut(s) 394, 593, 1075, 1076, 1876
BmrFI CCNGG 2 cut(s) 1383, 1566
BmsI GCATC 4 cut(s) 1612, 1670, 1720, 1908
BpiI GAAGAC 3 cut(s) 720, 943, 1814
BplI GAGNNNNNCTC 2 cut(s) 269, 301
BpmI CTGGAG 1 cut(s) 500
BpuEI CTTGAG 2 cut(s) 134, 477
BsaAI YACGTR 1 cut(s) 820
BsaI GGTCTC 1 cut(s) 894
BsaJI CCNNGG 2 cut(s) 595, 1672
Bsc4I CCNNNNNNNGG 6 cut(s) 800, 1163, 1662, 1786, 1894, 1939
Bse118I RCCGGY 1 cut(s) 1771
Bse1I ACTGG 3 cut(s) 542, 844, 1139
Bse3DI GCAATG 1 cut(s) 1277
BseBI CCWGG 2 cut(s) 1383, 1566
BseDI CCNNGG 2 cut(s) 595, 1672
BseGI GGATG 2 cut(s) 1153, 1685
BseLI CCNNNNNNNGG 6 cut(s) 800, 1163, 1662, 1786, 1894, 1939
BseMI GCAATG 1 cut(s) 1277
BseMII CTCAG 1 cut(s) 1108
BseNI ACTGG 3 cut(s) 542, 844, 1139
BseRI GAGGAG 1 cut(s) 912
BseXI GCAGC 2 cut(s) 115, 425
BsgI GTGCAG 1 cut(s) 1080
Bsh1236I CGCG 1 cut(s) 1035
BshFI GGCC 4 cut(s) 293, 1107, 1655, 1877
BshNI GGYRCC 1 cut(s) 392
BsiHKAI GWGCWC 1 cut(s) 444
BsiSI CCGG 1 cut(s) 1772
BslFI GGGAC 4 cut(s) 689, 1078, 1087, 1605
BslI CCNNNNNNNGG 6 cut(s) 800, 1163, 1662, 1786, 1894, 1939
BsmAI GTCTC 5 cut(s) 164, 530, 862, 894, 1251
BsmBI CGTCTC 1 cut(s) 1251
BsmFI GGGAC 4 cut(s) 689, 1078, 1087, 1605
BsmI GAATGC 2 cut(s) 570, 740
BsnI GGCC 4 cut(s) 293, 1107, 1655, 1877
Bso31I GGTCTC 1 cut(s) 894
Bsp1286I GDGCHC 1 cut(s) 444
Bsp1407I TGTACA 1 cut(s) 726
Bsp143I GATC 7 cut(s) 346, 591, 612, 814, 945, 1036, 1841
BspACI CCGC 8 cut(s) 396, 600, 875, 1287, 1472, 1475, 1497, 1894
BspANI GGCC 4 cut(s) 293, 1107, 1655, 1877
BspCNI CTCAG 1 cut(s) 1107
BspFNI CGCG 1 cut(s) 1035
BspLI GGNNCC 5 cut(s) 394, 593, 1075, 1076, 1876
BspMAI CTGCAG 1 cut(s) 162
BspMI ACCTGC 2 cut(s) 165, 1800
BspPI GGATC 3 cut(s) 586, 599, 822
BspT107I GGYRCC 1 cut(s) 392
BspTNI GGTCTC 1 cut(s) 894
BsrDI GCAATG 1 cut(s) 1277
BsrFI RCCGGY 1 cut(s) 1771
BsrGI TGTACA 1 cut(s) 726
BsrI ACTGG 3 cut(s) 542, 844, 1139
BssAI RCCGGY 1 cut(s) 1771
BssECI CCNNGG 2 cut(s) 595, 1672
BssMI GATC 7 cut(s) 346, 591, 612, 814, 945, 1036, 1841
BssNAI GTATAC 1 cut(s) 628
BssSI CACGAG 2 cut(s) 604, 1282
BssT1I CCWWGG 1 cut(s) 595
Bst1107I GTATAC 1 cut(s) 628
Bst2BI CACGAG 2 cut(s) 604, 1282
Bst2UI CCWGG 2 cut(s) 1383, 1566
Bst4CI ACNGT 6 cut(s) 44, 580, 757, 1170, 1243, 1676
Bst6I CTCTTC 1 cut(s) 961
BstAPI GCANNNNNTGC 1 cut(s) 929
BstAUI TGTACA 1 cut(s) 726
BstBAI YACGTR 1 cut(s) 820
BstC8I GCNNGC 3 cut(s) 622, 784, 1777
BstDEI CTNAG 1 cut(s) 1094
BstDSI CCRYGG 1 cut(s) 1672
BstF5I GGATG 2 cut(s) 1153, 1685
BstFNI CGCG 1 cut(s) 1035
BstKTI GATC 7 cut(s) 349, 594, 615, 817, 948, 1039, 1844
BstMAI GTCTC 5 cut(s) 164, 530, 862, 894, 1251
BstMBI GATC 7 cut(s) 346, 591, 612, 814, 945, 1036, 1841
BstMWI GCNNNNNNNGC 3 cut(s) 606, 929, 1481
BstNI CCWGG 2 cut(s) 1383, 1566
BstNSI RCATGY 2 cut(s) 875, 1718
BstSCI CCNGG 2 cut(s) 1381, 1564
BstSFI CTRYAG 4 cut(s) 158, 981, 1309, 1889
BstUI CGCG 1 cut(s) 1035
BstV1I GCAGC 2 cut(s) 115, 425
BstV2I GAAGAC 3 cut(s) 720, 943, 1814
BstX2I RGATCY 1 cut(s) 591
BstYI RGATCY 1 cut(s) 591
BstZ17I GTATAC 1 cut(s) 628
BsuI GTATCC 1 cut(s) 112
BsuRI GGCC 4 cut(s) 293, 1107, 1655, 1877
BtgI CCRYGG 1 cut(s) 1672
BtsCI GGATG 2 cut(s) 1153, 1685
BtsIMutI CAGTG 4 cut(s) 342, 622, 837, 1102
BveI ACCTGC 2 cut(s) 165, 1800
Cac8I GCNNGC 3 cut(s) 622, 784, 1777
CaiI CAGNNNCTG 1 cut(s) 173
Cfr10I RCCGGY 1 cut(s) 1771
Cfr13I GGNCC 7 cut(s) 1074, 1106, 1289, 1385, 1439, 1477, 1875
CseI GACGC 1 cut(s) 3
CsiI ACCWGGT 1 cut(s) 1564
Csp6I GTAC 6 cut(s) 154, 239, 393, 727, 821, 1031
CviQI GTAC 6 cut(s) 154, 239, 393, 727, 821, 1031
DdeI CTNAG 1 cut(s) 1094
DpnI GATC 7 cut(s) 348, 593, 614, 816, 947, 1038, 1843
DpnII GATC 7 cut(s) 346, 591, 612, 814, 945, 1036, 1841
DraI TTTAAA 1 cut(s) 1540
DriI GACNNNNNGTC 1 cut(s) 1437
EaeI YGGCCR 1 cut(s) 291
Eam1104I CTCTTC 1 cut(s) 961
Eam1105I GACNNNNNGTC 1 cut(s) 1437
EarI CTCTTC 1 cut(s) 961
EciI GGCGGA 1 cut(s) 1490
Eco130I CCWWGG 1 cut(s) 595
Eco31I GGTCTC 1 cut(s) 894
Eco47I GGWCC 5 cut(s) 1074, 1289, 1385, 1439, 1477
Eco57I CTGAAG 2 cut(s) 51, 72
EcoO109I RGGNCCY 2 cut(s) 1074, 1385
EcoRI GAATTC 1 cut(s) 204
EcoRII CCWGG 2 cut(s) 1381, 1564
EcoT14I CCWWGG 1 cut(s) 595
EcoT22I ATGCAT 6 cut(s) 9, 742, 1537, 1685, 1735, 1868
ErhI CCWWGG 1 cut(s) 595
Esp3I CGTCTC 1 cut(s) 1251
FalI AAGNNNNNCTT 8 cut(s) 115, 147, 133, 165, 935, 967, 1917, 1949
FaqI GGGAC 4 cut(s) 689, 1078, 1087, 1605
FauI CCCGC 1 cut(s) 1887
FauNDI CATATG 2 cut(s) 700, 1027
FbaI TGATCA 1 cut(s) 612
FblI GTMKAC 1 cut(s) 627
Fnu4HI GCNGC 6 cut(s) 129, 414, 601, 876, 1473, 1498
FokI GGATG 2 cut(s) 1140, 1692
Fsp4HI GCNGC 6 cut(s) 129, 414, 601, 876, 1473, 1498
FspBI CTAG 5 cut(s) 356, 905, 960, 1247, 1323
GluI GCNGC 6 cut(s) 129, 414, 601, 876, 1473, 1498
GsuI CTGGAG 1 cut(s) 500
HaeIII GGCC 4 cut(s) 293, 1107, 1655, 1877
HapII CCGG 1 cut(s) 1772
HgaI GACGC 1 cut(s) 3
HindIII AAGCTT 1 cut(s) 768
HpaII CCGG 1 cut(s) 1772
HphI GGTGA 2 cut(s) 1055, 1573
Hpy166II GTNNAC 3 cut(s) 283, 628, 727
Hpy188I TCNGA 9 cut(s) 70, 91, 203, 210, 304, 480, 562, 1426, 1800
Hpy188III TCNNGA 5 cut(s) 49, 487, 494, 663, 969
Hpy8I GTNNAC 3 cut(s) 283, 628, 727
HpyAV CCTTC 3 cut(s) 582, 1568, 1931
HpyCH4III ACNGT 6 cut(s) 44, 580, 757, 1170, 1243, 1676
HpyCH4IV ACGT 4 cut(s) 190, 819, 936, 1161
HpyF10VI GCNNNNNNNGC 3 cut(s) 606, 929, 1481
HpyF3I CTNAG 1 cut(s) 1094
HpySE526I ACGT 4 cut(s) 190, 819, 936, 1161
KflI GGGWCCC 1 cut(s) 1074
KpnI GGTACC 1 cut(s) 396
Ksp22I TGATCA 1 cut(s) 612
Kzo9I GATC 7 cut(s) 346, 591, 612, 814, 945, 1036, 1841
LmnI GCTCC 1 cut(s) 1306
Lsp1109I GCAGC 2 cut(s) 115, 425
LweI GCATC 4 cut(s) 1612, 1670, 1720, 1908
MabI ACCWGGT 1 cut(s) 1564
MaeI CTAG 5 cut(s) 356, 905, 960, 1247, 1323
MaeII ACGT 4 cut(s) 190, 819, 936, 1161
MaeIII GTNAC 4 cut(s) 757, 1237, 1429, 1765
MalI GATC 7 cut(s) 348, 593, 614, 816, 947, 1038, 1843
MboI GATC 7 cut(s) 346, 591, 612, 814, 945, 1036, 1841
MfeI CAATTG 3 cut(s) 1233, 1434, 1945
MflI RGATCY 1 cut(s) 591
MhlI GDGCHC 1 cut(s) 444
MlsI TGGCCA 1 cut(s) 293
MluNI TGGCCA 1 cut(s) 293
MlyI GAGTC 3 cut(s) 484, 996, 1121
MmeI TCCRAC 3 cut(s) 364, 1144, 1669
Mox20I TGGCCA 1 cut(s) 293
Mph1103I ATGCAT 6 cut(s) 9, 742, 1537, 1685, 1735, 1868
MscI TGGCCA 1 cut(s) 293
MseI TTAA 8 cut(s) 336, 654, 690, 891, 1200, 1523, 1539, 1719
MslI CAYNNNNRTG 5 cut(s) 608, 703, 722, 1395, 1719
Msp20I TGGCCA 1 cut(s) 293
MspA1I CMGCKG 1 cut(s) 1894
MspI CCGG 1 cut(s) 1772
MspR9I CCNGG 2 cut(s) 1383, 1566
MunI CAATTG 3 cut(s) 1233, 1434, 1945
Mva1269I GAATGC 2 cut(s) 570, 740
MvaI CCWGG 2 cut(s) 1383, 1566
MvnI CGCG 1 cut(s) 1035
MwoI GCNNNNNNNGC 3 cut(s) 606, 929, 1481
NdeI CATATG 2 cut(s) 700, 1027
NdeII GATC 7 cut(s) 346, 591, 612, 814, 945, 1036, 1841
NlaIV GGNNCC 5 cut(s) 394, 593, 1075, 1076, 1876
NmeAIII GCCGAG 1 cut(s) 1667
NmuCI GTSAC 2 cut(s) 1429, 1765
NsiI ATGCAT 6 cut(s) 9, 742, 1537, 1685, 1735, 1868
NspI RCATGY 2 cut(s) 875, 1718
OliI CACNNNNGTG 1 cut(s) 722
PctI GAATGC 2 cut(s) 570, 740
PfeI GAWTC 8 cut(s) 659, 804, 834, 965, 1001, 1452, 1646, 1748
PflMI CCANNNNNTGG 2 cut(s) 800, 1786
PkrI GCNGC 6 cut(s) 130, 415, 602, 877, 1474, 1499
PleI GAGTC 3 cut(s) 484, 995, 1120
PpsI GAGTC 3 cut(s) 484, 995, 1120
Ppu21I YACGTR 1 cut(s) 820
PpuMI RGGWCCY 2 cut(s) 1074, 1385
PshBI ATTAAT 1 cut(s) 336
Psp5II RGGWCCY 2 cut(s) 1074, 1385
Psp6I CCWGG 2 cut(s) 1381, 1564
PspGI CCWGG 2 cut(s) 1381, 1564
PspN4I GGNNCC 5 cut(s) 394, 593, 1075, 1076, 1876
PspPI GGNCC 7 cut(s) 1074, 1106, 1289, 1385, 1439, 1477, 1875
PspPPI RGGWCCY 2 cut(s) 1074, 1385
PstI CTGCAG 1 cut(s) 162
PstNI CAGNNNCTG 1 cut(s) 173
PsuI RGATCY 1 cut(s) 591
RsaI GTAC 6 cut(s) 155, 240, 394, 728, 822, 1032
RsaNI GTAC 6 cut(s) 154, 239, 393, 727, 821, 1031
RseI CAYNNNNRTG 5 cut(s) 608, 703, 722, 1395, 1719
SaqAI TTAA 8 cut(s) 336, 654, 690, 891, 1200, 1523, 1539, 1719
SatI GCNGC 6 cut(s) 129, 414, 601, 876, 1473, 1498
Sau3AI GATC 7 cut(s) 346, 591, 612, 814, 945, 1036, 1841
Sau96I GGNCC 7 cut(s) 1074, 1106, 1289, 1385, 1439, 1477, 1875
SbfI CCTGCAGG 1 cut(s) 162
SchI GAGTC 3 cut(s) 484, 996, 1121
ScrFI CCNGG 2 cut(s) 1383, 1566
SdaI CCTGCAGG 1 cut(s) 162
SduI GDGCHC 1 cut(s) 444
SexAI ACCWGGT 1 cut(s) 1564
SfaNI GCATC 4 cut(s) 1612, 1670, 1720, 1908
SfcI CTRYAG 4 cut(s) 158, 981, 1309, 1889
SgrAI CRCCGGYG 1 cut(s) 1771
SinI GGWCC 5 cut(s) 1074, 1289, 1385, 1439, 1477
SmiMI CAYNNNNRTG 5 cut(s) 608, 703, 722, 1395, 1719
SmlI CTYRAG 2 cut(s) 113, 492
SmoI CTYRAG 2 cut(s) 113, 492
Sse8387I CCTGCAGG 1 cut(s) 162
SsiI CCGC 8 cut(s) 396, 600, 875, 1287, 1472, 1475, 1497, 1894
SspMI CTAG 5 cut(s) 356, 905, 960, 1247, 1323
StyD4I CCNGG 2 cut(s) 1381, 1564
StyI CCWWGG 1 cut(s) 595
TaaI ACNGT 6 cut(s) 44, 580, 757, 1170, 1243, 1676
TaiI ACGT 4 cut(s) 193, 822, 939, 1164
TaqI TCGA 1 cut(s) 1629
TatI WGTACW 2 cut(s) 238, 726
TauI GCSGC 4 cut(s) 603, 878, 1475, 1500
TfiI GAWTC 8 cut(s) 659, 804, 834, 965, 1001, 1452, 1646, 1748
Tru1I TTAA 8 cut(s) 336, 654, 690, 891, 1200, 1523, 1539, 1719
Tru9I TTAA 8 cut(s) 336, 654, 690, 891, 1200, 1523, 1539, 1719
TscAI CASTG 4 cut(s) 349, 622, 844, 1102
TseFI GTSAC 2 cut(s) 1429, 1765
TseI GCWGC 2 cut(s) 128, 413
Tsp45I GTSAC 2 cut(s) 1429, 1765
TspDTI ATGAA 9 cut(s) 636, 672, 897, 917, 1186, 1407, 1517, 1638, 1931
TspGWI ACGGA 1 cut(s) 1023
TspRI CASTG 4 cut(s) 349, 622, 844, 1102
Van91I CCANNNNNTGG 2 cut(s) 800, 1786
VpaK11BI GGWCC 5 cut(s) 1074, 1289, 1385, 1439, 1477
VspI ATTAAT 1 cut(s) 336
XapI RAATTY 6 cut(s) 204, 1011, 1348, 1414, 1827, 1918
XceI RCATGY 2 cut(s) 875, 1718
XcmI CCANNNNNNNNNTGG 1 cut(s) 1160
XmiI GTMKAC 1 cut(s) 627
XspI CTAG 5 cut(s) 356, 905, 960, 1247, 1323
Zsp2I ATGCAT 6 cut(s) 9, 742, 1537, 1685, 1735, 1868
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.