RchiOBHm_Chr4g0403781

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000533
Physical Location & Seq
Reverse (-)
23244353 .. 23249056
4704 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37546

Sequence Viewer

Length: 1500 bp
ATGAAGTTTGTGCGCTATGCCACCGATCTACCGCTAGAAGAAGAATACGACTACATTGTAGTTGGGGGAGGAACAGCAGGCTGTCCATTGGCAACAACTTTATCCGAAAAGTATTCAGTATTGGTTCTAGAAAGAGGCAGTGTTCCTAGTGCATATCCAAGTGTCTTGCGTGAAGAAAAGTTTGCGAGTAATGTCATGCAGGAAGATGATGGTAAGATCCCAGCTCAAAGGTTCACCTCAGAAGATGGTGTTCGTAATGCAAGAGGCAGGATCTTGGGTGGGTCGAGTATGATCAATGCTGGTTTTTACTCCAGAGCCGAAAAGGAGTTCTATCTCACATCTGGAATTGAATGGGATATGGATATGGTTTATAAGGCATATACTTGGGTCGAAAACACCACCGTATTCCGCCCGGTTAATGTGGAAACTTGGCAATCTGTTGTGAAAGAAGCCTTATTGGAGGCTGGTGTTACTCCGGACAATGGATTTAATTTGGAACACATTCCAGGAGCTAAAGTTGGGGGTTCGATTTTTGACAGTAATGGAAGGAGGCATGGAGCTGTTGAACTGCTTAATAGAGCAGAGCCAAACAACTTGCAAGTTGCAGTTCATGCCACAGTAGAGAGGATCATCTTTTCTTCCAATGTGTCAAGTTGCTCTGCCACAGGAATTATATACAGTGATTCTGACGGGAGGTCACACCGGGCATTTGTACGTGGTAAAGGAGAGGTTATATTGAGTGCTGGAACGATTGGGAGCCCTCAACTTCTGCTACTCAGTGGTGTTGGTCCTCAATCCTACCTTTCGTCTCTTAAAATCCCGGTCATCTACCCTCAACCCAACGTTGGACAGTTTATGTTCGACAATCCTCGTAATTCCATAAACATTTTGCCCCCATTTCTACTTCAGCCTTCATTTCTACAAATTGCTGGAATTGCAACTGATTTCTACATAGAGGCTCTCTCTTATATCGTGCCAAATAATTCCAGCAATGTGACTTTGGCAAATATTGCTTCGAAAGTACCTGGACCCTTATCATATGGTTCTCTCCGGTTGCAATCATCTTCTGACGTGAGAGTCGGGCCGAATGTCAGCTTCAACTACTTTGCACATCCGGCCGACCTTGCTCGCTGCGTTAAGGGACTGAGAAAGATTGCAGACTTATTAAAGACAAACTCGTTGAAACCATTTAGGTCCCAAAATTCTTCGGGTACGGAAGGTTTTAACTTTTATGGACCATCTCTACCTACGAACCAAGCAGATGATGCTCCCTTTGAAACCTTCTGTCGAACTACAGTTGCCACATTTTGGCATTACCATGGCGGATGCCTTGTGGGAAAGGTGGTCGATAGCAGTTTGCGAGTCATTGGGATCAATTCATTACGTGTTGTTGATGGTTCCACATTCAATTTCTCACCAGGAACCAATCCCCAGGCCACCGTTATGATGTTAGGCAGGTATGTTGGCCTTAAGATGCTGCAAGAAAGAGAAGTTAATTGA

Protein Analysis

499

Amino Acids

54.41

Weight (kDa)

6.42

Isoelectric Point (pI)

44.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_N PF00732 16 - 286 4.2e-21 GMC oxidoreductase
GMC_oxred_C PF05199 344 - 485 3.9e-27 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 372
AasI GACNNNNNNGTC 1 cut(s) 1076
Acc36I ACCTGC 1 cut(s) 1446
AccB7I CCANNNNNTGG 1 cut(s) 1308
AccIII TCCGGA 1 cut(s) 475
AciI CCGC 3 cut(s) 32, 409, 1323
AclI AACGTT 1 cut(s) 843
AclWI GGATC 4 cut(s) 211, 278, 635, 1379
AcoI YGGCCR 1 cut(s) 1116
AcsI RAATTY 1 cut(s) 1201
AcuI CTGAAG 1 cut(s) 890
AfaI GTAC 3 cut(s) 714, 1023, 1213
AfiI CCNNNNNNNGG 3 cut(s) 482, 845, 1308
AflII CTTAAG 1 cut(s) 1469
AflIII ACRYGT 1 cut(s) 1384
AgsI TTSAA 6 cut(s) 350, 566, 1099, 1183, 1277, 1408
AhdI GACNNNNNGTC 1 cut(s) 694
AjiI CACGTC 1 cut(s) 1072
AjnI CCWGG 4 cut(s) 505, 1024, 1417, 1431
AluBI AGCT 4 cut(s) 224, 512, 560, 1095
AluI AGCT 4 cut(s) 224, 512, 560, 1095
Alw26I GTCTC 1 cut(s) 813
AlwI GGATC 4 cut(s) 211, 278, 635, 1379
Aor13HI TCCGGA 1 cut(s) 475
AoxI GGCC 4 cut(s) 1082, 1116, 1434, 1465
ApeKI GCWGC 2 cut(s) 1131, 1477
ApoI RAATTY 1 cut(s) 1201
Asp700I GAANNNNTTC 1 cut(s) 501
AspLEI GCGC 1 cut(s) 15
AspS9I GGNCC 5 cut(s) 788, 1028, 1082, 1194, 1235
AsuC2I CCSGG 3 cut(s) 413, 704, 821
AsuHPI GGTGA 2 cut(s) 226, 1407
AsuII TTCGAA 1 cut(s) 1016
AvaII GGWCC 4 cut(s) 788, 1028, 1194, 1235
BanII GRGCYC 1 cut(s) 761
BbvI GCAGC 2 cut(s) 1118, 1464
BccI CCATC 4 cut(s) 203, 239, 1246, 1388
BciT130I CCWGG 4 cut(s) 507, 1026, 1419, 1433
BclI TGATCA 1 cut(s) 291
BcnI CCSGG 3 cut(s) 413, 704, 821
BcoDI GTCTC 1 cut(s) 813
BfaI CTAG 3 cut(s) 35, 128, 147
BfmI CTRYAG 1 cut(s) 1293
BfrI CTTAAG 1 cut(s) 1469
BfuAI ACCTGC 1 cut(s) 1446
BisI GCNGC 2 cut(s) 1132, 1478
BlsI GCNGC 2 cut(s) 1133, 1479
Bme1390I CCNGG 7 cut(s) 413, 507, 704, 821, 1026, 1419, 1433
Bme18I GGWCC 4 cut(s) 788, 1028, 1194, 1235
BmeRI GACNNNNNGTC 1 cut(s) 694
BmgBI CACGTC 1 cut(s) 1072
BmgT120I GGNCC 5 cut(s) 788, 1028, 1082, 1194, 1235
BmiI GGNNCC 5 cut(s) 758, 1030, 1196, 1399, 1423
BmrFI CCNGG 7 cut(s) 413, 507, 704, 821, 1026, 1419, 1433
BmsI GCATC 3 cut(s) 1255, 1316, 1464
BplI GAGNNNNNCTC 2 cut(s) 947, 979
BpmI CTGGAG 1 cut(s) 295
Bpu14I TTCGAA 1 cut(s) 1016
BpuMI CCSGG 3 cut(s) 413, 704, 821
BsaAI YACGTR 2 cut(s) 716, 1385
BsaJI CCNNGG 2 cut(s) 1318, 1431
BsaWI WCCGGW 2 cut(s) 475, 1050
BsaXI ACNNNNNCTCC 2 cut(s) 501, 531
Bsc4I CCNNNNNNNGG 3 cut(s) 482, 845, 1308
Bse3DI GCAATG 1 cut(s) 997
BseAI TCCGGA 1 cut(s) 475
BseBI CCWGG 4 cut(s) 507, 1026, 1419, 1433
BseDI CCNNGG 2 cut(s) 1318, 1431
BseGI GGATG 2 cut(s) 1111, 1331
BseLI CCNNNNNNNGG 3 cut(s) 482, 845, 1308
BseMI GCAATG 1 cut(s) 997
BseMII CTCAG 3 cut(s) 252, 790, 1136
BseX3I CGGCCG 1 cut(s) 1116
BseXI GCAGC 2 cut(s) 1118, 1464
BseYI CCCAGC 1 cut(s) 220
Bsh1285I CGRYCG 1 cut(s) 1119
BshFI GGCC 4 cut(s) 1084, 1118, 1436, 1467
BsiEI CGRYCG 1 cut(s) 1119
BsiSI CCGG 6 cut(s) 413, 476, 703, 821, 1051, 1115
BslFI GGGAC 2 cut(s) 1155, 1180
BslI CCNNNNNNNGG 3 cut(s) 482, 845, 1308
BsmAI GTCTC 1 cut(s) 813
BsmBI CGTCTC 1 cut(s) 813
BsmFI GGGAC 2 cut(s) 1155, 1180
BsnI GGCC 4 cut(s) 1084, 1118, 1436, 1467
Bsp119I TTCGAA 1 cut(s) 1016
Bsp1286I GDGCHC 1 cut(s) 761
Bsp13I TCCGGA 1 cut(s) 475
Bsp143I GATC 6 cut(s) 25, 216, 270, 291, 627, 1371
Bsp19I CCATGG 1 cut(s) 1318
BspACI CCGC 3 cut(s) 32, 409, 1323
BspANI GGCC 4 cut(s) 1084, 1118, 1436, 1467
BspCNI CTCAG 3 cut(s) 251, 789, 1137
BspEI TCCGGA 1 cut(s) 475
BspLI GGNNCC 5 cut(s) 758, 1030, 1196, 1399, 1423
BspMI ACCTGC 1 cut(s) 1446
BspPI GGATC 4 cut(s) 211, 278, 635, 1379
BspT104I TTCGAA 1 cut(s) 1016
BspTI CTTAAG 1 cut(s) 1469
BsrDI GCAATG 1 cut(s) 997
BssECI CCNNGG 2 cut(s) 1318, 1431
BssMI GATC 6 cut(s) 25, 216, 270, 291, 627, 1371
BssT1I CCWWGG 1 cut(s) 1318
Bst2UI CCWGG 4 cut(s) 507, 1026, 1419, 1433
Bst4CI ACNGT 7 cut(s) 403, 539, 619, 680, 852, 1297, 1441
BstAFI CTTAAG 1 cut(s) 1469
BstAPI GCANNNNNTGC 3 cut(s) 611, 1010, 1265
BstBAI YACGTR 2 cut(s) 716, 1385
BstBI TTCGAA 1 cut(s) 1016
BstC8I GCNNGC 2 cut(s) 79, 1129
BstDEI CTNAG 3 cut(s) 238, 776, 1145
BstDSI CCRYGG 1 cut(s) 1318
BstF5I GGATG 2 cut(s) 1111, 1331
BstHHI GCGC 1 cut(s) 15
BstKTI GATC 6 cut(s) 28, 219, 273, 294, 630, 1374
BstMAI GTCTC 1 cut(s) 813
BstMBI GATC 6 cut(s) 25, 216, 270, 291, 627, 1371
BstMCI CGRYCG 1 cut(s) 1119
BstMWI GCNNNNNNNGC 6 cut(s) 611, 935, 1010, 1115, 1124, 1265
BstNI CCWGG 4 cut(s) 507, 1026, 1419, 1433
BstSCI CCNGG 7 cut(s) 411, 505, 702, 819, 1024, 1417, 1431
BstSFI CTRYAG 1 cut(s) 1293
BstV1I GCAGC 2 cut(s) 1118, 1464
BstX2I RGATCY 2 cut(s) 216, 270
BstYI RGATCY 2 cut(s) 216, 270
BstZI CGGCCG 1 cut(s) 1116
BsuRI GGCC 4 cut(s) 1084, 1118, 1436, 1467
BtgI CCRYGG 1 cut(s) 1318
BtrI CACGTC 1 cut(s) 1072
BtsCI GGATG 2 cut(s) 1111, 1331
BtsI GCAGTG 1 cut(s) 145
BtsIMutI CAGTG 3 cut(s) 145, 685, 784
BveI ACCTGC 1 cut(s) 1446
Cac8I GCNNGC 2 cut(s) 79, 1129
CfoI GCGC 1 cut(s) 15
Cfr13I GGNCC 5 cut(s) 788, 1028, 1082, 1194, 1235
Csp6I GTAC 3 cut(s) 713, 1022, 1212
CviAII CATG 4 cut(s) 196, 554, 611, 1319
CviQI GTAC 3 cut(s) 713, 1022, 1212
DdeI CTNAG 3 cut(s) 238, 776, 1145
DpnI GATC 6 cut(s) 27, 218, 272, 293, 629, 1373
DpnII GATC 6 cut(s) 25, 216, 270, 291, 627, 1371
DrdI GACNNNNNNGTC 1 cut(s) 1076
DriI GACNNNNNGTC 1 cut(s) 694
DseDI GACNNNNNNGTC 1 cut(s) 1076
EaeI YGGCCR 1 cut(s) 1116
EagI CGGCCG 1 cut(s) 1116
Eam1105I GACNNNNNGTC 1 cut(s) 694
EciI GGCGGA 2 cut(s) 398, 1338
EclXI CGGCCG 1 cut(s) 1116
Eco130I CCWWGG 1 cut(s) 1318
Eco24I GRGCYC 1 cut(s) 761
Eco47I GGWCC 4 cut(s) 788, 1028, 1194, 1235
Eco52I CGGCCG 1 cut(s) 1116
Eco57I CTGAAG 1 cut(s) 890
EcoO109I RGGNCCY 1 cut(s) 1194
EcoRII CCWGG 4 cut(s) 505, 1024, 1417, 1431
EcoT14I CCWWGG 1 cut(s) 1318
EcoT38I GRGCYC 1 cut(s) 761
ErhI CCWWGG 1 cut(s) 1318
Esp3I CGTCTC 1 cut(s) 813
FaeI CATG 4 cut(s) 199, 557, 614, 1322
FaqI GGGAC 2 cut(s) 1155, 1180
FatI CATG 4 cut(s) 195, 553, 610, 1318
FauNDI CATATG 1 cut(s) 1039
FbaI TGATCA 1 cut(s) 291
Fnu4HI GCNGC 2 cut(s) 1132, 1478
FokI GGATG 2 cut(s) 1098, 1338
FriOI GRGCYC 1 cut(s) 761
Fsp4HI GCNGC 2 cut(s) 1132, 1478
FspBI CTAG 3 cut(s) 35, 128, 147
GlaI GCGC 1 cut(s) 14
GluI GCNGC 2 cut(s) 1132, 1478
GsaI CCCAGC 1 cut(s) 224
GsuI CTGGAG 1 cut(s) 295
HaeIII GGCC 4 cut(s) 1084, 1118, 1436, 1467
HapII CCGG 6 cut(s) 413, 476, 703, 821, 1051, 1115
HhaI GCGC 1 cut(s) 15
Hin1II CATG 4 cut(s) 199, 557, 614, 1322
Hin6I GCGC 1 cut(s) 13
HinP1I GCGC 1 cut(s) 13
HinfI GANTC 3 cut(s) 683, 1077, 1362
HpaII CCGG 6 cut(s) 413, 476, 703, 821, 1051, 1115
HphI GGTGA 2 cut(s) 226, 1407
Hpy166II GTNNAC 1 cut(s) 234
Hpy188I TCNGA 4 cut(s) 106, 241, 688, 1069
Hpy188III TCNNGA 4 cut(s) 128, 312, 342, 476
Hpy8I GTNNAC 1 cut(s) 234
HpyAV CCTTC 4 cut(s) 540, 921, 1211, 1291
HpyCH4III ACNGT 7 cut(s) 403, 539, 619, 680, 852, 1297, 1441
HpyCH4IV ACGT 4 cut(s) 715, 843, 1071, 1384
HpyF10VI GCNNNNNNNGC 6 cut(s) 611, 935, 1010, 1115, 1124, 1265
HpyF3I CTNAG 3 cut(s) 238, 776, 1145
HpySE526I ACGT 4 cut(s) 715, 843, 1071, 1384
Hsp92II CATG 4 cut(s) 199, 557, 614, 1322
HspAI GCGC 1 cut(s) 13
Kpn2I TCCGGA 1 cut(s) 475
Ksp22I TGATCA 1 cut(s) 291
Kzo9I GATC 6 cut(s) 25, 216, 270, 291, 627, 1371
LmnI GCTCC 4 cut(s) 509, 557, 756, 1273
Lsp1109I GCAGC 2 cut(s) 1118, 1464
LweI GCATC 3 cut(s) 1255, 1316, 1464
MaeI CTAG 3 cut(s) 35, 128, 147
MaeII ACGT 4 cut(s) 715, 843, 1071, 1384
MaeIII GTNAC 3 cut(s) 469, 696, 994
MalI GATC 6 cut(s) 27, 218, 272, 293, 629, 1373
MboI GATC 6 cut(s) 25, 216, 270, 291, 627, 1371
MboII GAAGA 8 cut(s) 50, 53, 185, 215, 254, 630, 1056, 1197
MflI RGATCY 2 cut(s) 216, 270
MhlI GDGCHC 1 cut(s) 761
MlyI GAGTC 2 cut(s) 1086, 1371
MmeI TCCRAC 1 cut(s) 826
MroI TCCGGA 1 cut(s) 475
MroXI GAANNNNTTC 1 cut(s) 501
MseI TTAA 9 cut(s) 417, 489, 573, 813, 1137, 1166, 1224, 1470, 1494
MslI CAYNNNNRTG 2 cut(s) 1317, 1442
MspCI CTTAAG 1 cut(s) 1469
MspI CCGG 6 cut(s) 413, 476, 703, 821, 1051, 1115
MspR9I CCNGG 7 cut(s) 413, 507, 704, 821, 1026, 1419, 1433
MvaI CCWGG 4 cut(s) 507, 1026, 1419, 1433
MwoI GCNNNNNNNGC 6 cut(s) 611, 935, 1010, 1115, 1124, 1265
NciI CCSGG 3 cut(s) 413, 704, 821
NcoI CCATGG 1 cut(s) 1318
NdeI CATATG 1 cut(s) 1039
NdeII GATC 6 cut(s) 25, 216, 270, 291, 627, 1371
NlaIII CATG 4 cut(s) 199, 557, 614, 1322
NlaIV GGNNCC 5 cut(s) 758, 1030, 1196, 1399, 1423
NmuCI GTSAC 2 cut(s) 696, 994
NspV TTCGAA 1 cut(s) 1016
PdmI GAANNNNTTC 1 cut(s) 501
PfeI GAWTC 1 cut(s) 683
PflMI CCANNNNNTGG 1 cut(s) 1308
PfoI TCCNGGA 1 cut(s) 505
PkrI GCNGC 2 cut(s) 1133, 1479
PleI GAGTC 2 cut(s) 1085, 1370
PpsI GAGTC 2 cut(s) 1085, 1370
Ppu21I YACGTR 2 cut(s) 716, 1385
PpuMI RGGWCCY 1 cut(s) 1194
PsiI TTATAA 1 cut(s) 372
Psp1406I AACGTT 1 cut(s) 843
Psp5II RGGWCCY 1 cut(s) 1194
Psp6I CCWGG 4 cut(s) 505, 1024, 1417, 1431
PspFI CCCAGC 1 cut(s) 220
PspGI CCWGG 4 cut(s) 505, 1024, 1417, 1431
PspN4I GGNNCC 5 cut(s) 758, 1030, 1196, 1399, 1423
PspPI GGNCC 5 cut(s) 788, 1028, 1082, 1194, 1235
PspPPI RGGWCCY 1 cut(s) 1194
PsuI RGATCY 2 cut(s) 216, 270
RsaI GTAC 3 cut(s) 714, 1023, 1213
RsaNI GTAC 3 cut(s) 713, 1022, 1212
RseI CAYNNNNRTG 2 cut(s) 1317, 1442
SaqAI TTAA 9 cut(s) 417, 489, 573, 813, 1137, 1166, 1224, 1470, 1494
SatI GCNGC 2 cut(s) 1132, 1478
Sau3AI GATC 6 cut(s) 25, 216, 270, 291, 627, 1371
Sau96I GGNCC 5 cut(s) 788, 1028, 1082, 1194, 1235
SchI GAGTC 2 cut(s) 1086, 1371
ScrFI CCNGG 7 cut(s) 413, 507, 704, 821, 1026, 1419, 1433
SduI GDGCHC 1 cut(s) 761
SfaNI GCATC 3 cut(s) 1255, 1316, 1464
SfcI CTRYAG 1 cut(s) 1293
SfuI TTCGAA 1 cut(s) 1016
SinI GGWCC 4 cut(s) 788, 1028, 1194, 1235
SmiMI CAYNNNNRTG 2 cut(s) 1317, 1442
SmlI CTYRAG 1 cut(s) 1469
SmoI CTYRAG 1 cut(s) 1469
SsiI CCGC 3 cut(s) 32, 409, 1323
SspI AATATT 1 cut(s) 1009
SspMI CTAG 3 cut(s) 35, 128, 147
StyD4I CCNGG 7 cut(s) 411, 505, 702, 819, 1024, 1417, 1431
StyI CCWWGG 1 cut(s) 1318
TaaI ACNGT 7 cut(s) 403, 539, 619, 680, 852, 1297, 1441
TaiI ACGT 4 cut(s) 718, 846, 1074, 1387
TaqI TCGA 7 cut(s) 284, 390, 527, 861, 1016, 1288, 1347
TfiI GAWTC 1 cut(s) 683
Tru1I TTAA 9 cut(s) 417, 489, 573, 813, 1137, 1166, 1224, 1470, 1494
Tru9I TTAA 9 cut(s) 417, 489, 573, 813, 1137, 1166, 1224, 1470, 1494
TscAI CASTG 3 cut(s) 145, 685, 784
TseFI GTSAC 2 cut(s) 696, 994
TseI GCWGC 2 cut(s) 1131, 1477
Tsp45I GTSAC 2 cut(s) 696, 994
TspDTI ATGAA 4 cut(s) 17, 599, 903, 1368
TspGWI ACGGA 1 cut(s) 1229
TspRI CASTG 3 cut(s) 145, 685, 784
Van91I CCANNNNNTGG 1 cut(s) 1308
Vha464I CTTAAG 1 cut(s) 1469
VpaK11BI GGWCC 4 cut(s) 788, 1028, 1194, 1235
XapI RAATTY 1 cut(s) 1201
XbaI TCTAGA 1 cut(s) 127
XmnI GAANNNNTTC 1 cut(s) 501
XspI CTAG 3 cut(s) 35, 128, 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.