MD00G1012800.v1.1

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000533
Physical Location & Seq
Reverse (-)
1583301 .. 1589619
6319 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1012800.v1.1.491

Sequence Viewer

Length: 1662 bp
ATGGAGAAATCAGCAATTGCTACTAGATTATTGCTTTTGTACTTTTTTGTCTTCTGCCCTCAATTAGGGGTTCACTCATTCGCCACTCCTTCTAACATTCAAGATTTTAGCTACTTGAAATTTGTACGTAACGCAACTGATCTACCATTGCAAGAAAAATATGACTACATTGTGGTTGGAGGGGGCACGGCAGGGTGCCCATTGGCAGCAACTTTATCCGCGAACTACTCCGTGCTCCTCCTAGAAAGGGGAGATATTCCCACAGCATATCCAAACGTGTTGAGTAACGCTGGGATTCTTGCAAATTTCATGCAAGAAGATGACGGTAAGACGCCAGCCCAGAGGTTCACATCAGAAGATGGTGTAGCTTTTGTAAGAGGACGGGTCCTAGGCGGGTCAAGTATGATCAATATTGGTCTTTACTCAAGGGCCGACAGTGAATTCCTCAAGAAATCAGGAATTAAATTGGACATGAACTTAGTCAATAATTCATATGAATGGGTTGAAAACACTCTAGTGTTCCGTCCGAATTTATCACACTGGCAATCTGTTGTGAAAGATGCATTGTTAGAAGCTGGTGTTCGTCCAGACAATGGACTTACTTTGGATCACATTCAGGGAACTAAAATCACGGGTACGATCTTTGACGATCGTGGAAGAAGACATGGTGCCGTGGAACTGCTAAATAAAGGACATCCAAAAAACCTGAGAGTTGCAATTCATGCCGCAGTAGAGAGGATCATTTTCTCTTCCAAGGCATCAGGTTTGTCAGCTAAAGGAATCATATACACTGATTCCTATGGGAGGTCTCATCAGGCATTGATACGTGGTAAAGGTGAGGTTATATTGAGTGCAGGGGCAATTGGGAGTCCTCAACTTCTGCTACTTAGTGGTGTTGGCCCGAAGTCCTACCTTTCATCTCTTAAAATCCCAGTTGTTCACCCACAACCTTACGTTGGGCAGTTTATGCGTGACAATCCTCGTAATTACGTTACCATTTTGCCCCCATCTGAACTAGAACCTTCTACTGCACAGATTGCTGGTATCACAAGTGATTACTATATAGAGACCGTCTCCGGGTTGCCATTTTCTACTATGGCCTTTAGTCTTTTCCCTAATCCAACTATTCCCATGACGATAAATTCAACTTTCGGGCACATTGTTACCAAAAATCCAGCACCCTTGTCCTATGGTTCTCTTAAACTGCAATCATCCTATGATGTCAAAGTCGGCCCAAATGTCAAATTCAACTACTTTGCAAACAAGGCAGACCTTTCTCGTTGTGTTAGTGCCGTGAGGAAAATGGGTGATTTATTAAAAACAAACGCATTGAAACCATTTAAGACTCAAGATTTGCCAGGCGAAGATGGTTTTAACCTTTTTGGACCGCCTTTACCAATGAACCGGTCAGATGTTGCATCCATTGAAACATTCTGTCGAGACACAGTAGCCACATTTTGGCACTACCATGGTGGATGCCTCGTCGGAAAGGTAGTTGATGGAGATTTCCGGGTCATGGGGATCAAGGCATTACGTGTTGTCGATGGATCTGTGTTCAAAAGTTTATCACCAGGGACCAATCCTCAAGCCACTCTTATGATGTTAGGCAGGTATGTTGGCCTTCGATTGCTGGAAGAAAGATCAGCTAGCAAGGGGCGTTGA

Protein Analysis

554

Amino Acids

60.27

Weight (kDa)

9.21

Isoelectric Point (pI)

33.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_N PF00732 54 - 321 1.8e-20 GMC oxidoreductase
GMC_oxred_C PF05199 394 - 536 3.3e-24 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1599
AccB1I GGYRCC 2 cut(s) 195, 668
AccB7I CCANNNNNTGG 2 cut(s) 593, 1458
AccII CGCG 1 cut(s) 221
AciI CCGC 4 cut(s) 219, 393, 726, 1388
AclWI GGATC 4 cut(s) 615, 746, 1529, 1555
AcsI RAATTY 6 cut(s) 119, 304, 440, 529, 1141, 1244
AcyI GRCGYC 1 cut(s) 332
AfaI GTAC 3 cut(s) 41, 126, 637
AfiI CCNNNNNNNGG 8 cut(s) 65, 247, 593, 804, 956, 1077, 1458, 1516
AflIII ACRYGT 2 cut(s) 276, 1534
AgeI ACCGGT 1 cut(s) 1404
AgsI TTSAA 8 cut(s) 101, 118, 506, 1146, 1249, 1333, 1427, 1558
AjnI CCWGG 2 cut(s) 1357, 1570
AleI CACNNNNGTG 1 cut(s) 515
AluBI AGCT 5 cut(s) 111, 368, 575, 773, 1646
AluI AGCT 5 cut(s) 111, 368, 575, 773, 1646
Alw21I GWGCWC 1 cut(s) 237
Alw26I GTCTC 4 cut(s) 813, 1061, 1078, 1434
AlwI GGATC 4 cut(s) 615, 746, 1529, 1555
AoxI GGCC 5 cut(s) 429, 898, 1098, 1231, 1618
ApeKI GCWGC 1 cut(s) 206
ApoI RAATTY 6 cut(s) 119, 304, 440, 529, 1141, 1244
ArsI GACNNNNNNTTYG 2 cut(s) 1336, 1368
AsiGI ACCGGT 1 cut(s) 1404
AspA2I CCTAGG 1 cut(s) 388
AspS9I GGNCC 6 cut(s) 385, 429, 899, 1232, 1385, 1575
AsuC2I CCSGG 2 cut(s) 1078, 1511
AsuHPI GGTGA 4 cut(s) 848, 932, 1319, 1560
AsuNHI GCTAGC 1 cut(s) 1646
AvaII GGWCC 3 cut(s) 385, 1385, 1575
AvrII CCTAGG 1 cut(s) 388
BaeGI GKGCMC 3 cut(s) 188, 200, 1158
BanI GGYRCC 2 cut(s) 195, 668
BbsI GAAGAC 2 cut(s) 43, 667
Bbv12I GWGCWC 1 cut(s) 237
BbvI GCAGC 1 cut(s) 218
BccI CCATC 5 cut(s) 353, 1015, 1361, 1493, 1538
BceAI ACGGC 3 cut(s) 204, 656, 1277
BciT130I CCWGG 2 cut(s) 1359, 1572
BclI TGATCA 1 cut(s) 405
BcnI CCSGG 2 cut(s) 1078, 1511
BcoDI GTCTC 4 cut(s) 813, 1061, 1078, 1434
BfaI CTAG 6 cut(s) 24, 242, 389, 515, 1016, 1647
BfuAI ACCTGC 1 cut(s) 1599
BisI GCNGC 2 cut(s) 207, 726
BlnI CCTAGG 1 cut(s) 388
BlsI GCNGC 2 cut(s) 208, 727
Bme1390I CCNGG 4 cut(s) 1078, 1359, 1511, 1572
Bme18I GGWCC 3 cut(s) 385, 1385, 1575
BmgT120I GGNCC 6 cut(s) 385, 429, 899, 1232, 1385, 1575
BmiI GGNNCC 4 cut(s) 197, 386, 670, 1576
BmrFI CCNGG 4 cut(s) 1078, 1359, 1511, 1572
BmrI ACTGGG 1 cut(s) 926
BmsI GCATC 4 cut(s) 550, 767, 1427, 1466
BmtI GCTAGC 1 cut(s) 1650
BmuI ACTGGG 1 cut(s) 926
BpiI GAAGAC 2 cut(s) 43, 667
BplI GAGNNNNNCTC 2 cut(s) 1058, 1090
BpuEI CTTGAG 4 cut(s) 409, 431, 1332, 1569
BpuMI CCSGG 2 cut(s) 1078, 1511
BsaAI YACGTR 3 cut(s) 128, 827, 1535
BsaHI GRCGYC 1 cut(s) 332
BsaI GGTCTC 2 cut(s) 813, 1061
BsaJI CCNNGG 5 cut(s) 388, 672, 753, 1468, 1571
BsaWI WCCGGW 1 cut(s) 1404
Bsc4I CCNNNNNNNGG 8 cut(s) 65, 247, 593, 804, 956, 1077, 1458, 1516
Bse118I RCCGGY 1 cut(s) 1404
Bse1I ACTGG 2 cut(s) 545, 932
Bse3DI GCAATG 1 cut(s) 146
BseBI CCWGG 2 cut(s) 1359, 1572
BseDI CCNNGG 5 cut(s) 388, 672, 753, 1468, 1571
BseGI GGATG 4 cut(s) 694, 1211, 1418, 1481
BseLI CCNNNNNNNGG 8 cut(s) 65, 247, 593, 804, 956, 1077, 1458, 1516
BseMI GCAATG 1 cut(s) 146
BseMII CTCAG 1 cut(s) 698
BseNI ACTGG 2 cut(s) 545, 932
BseRI GAGGAG 1 cut(s) 227
BseSI GKGCMC 3 cut(s) 188, 200, 1158
BseXI GCAGC 1 cut(s) 218
BseYI CCCAGC 1 cut(s) 290
BsgI GTGCAG 2 cut(s) 873, 1014
Bsh1236I CGCG 1 cut(s) 221
Bsh1285I CGRYCG 1 cut(s) 652
BshFI GGCC 5 cut(s) 431, 900, 1100, 1233, 1620
BshNI GGYRCC 2 cut(s) 195, 668
BshTI ACCGGT 1 cut(s) 1404
BsiEI CGRYCG 1 cut(s) 652
BsiHKAI GWGCWC 1 cut(s) 237
BsiSI CCGG 3 cut(s) 1077, 1405, 1510
BslFI GGGAC 1 cut(s) 1588
BslI CCNNNNNNNGG 8 cut(s) 65, 247, 593, 804, 956, 1077, 1458, 1516
BsmAI GTCTC 4 cut(s) 813, 1061, 1078, 1434
BsmBI CGTCTC 1 cut(s) 1078
BsmFI GGGAC 1 cut(s) 1588
BsnI GGCC 5 cut(s) 431, 900, 1100, 1233, 1620
Bso31I GGTCTC 2 cut(s) 813, 1061
Bsp1286I GDGCHC 4 cut(s) 188, 200, 237, 1158
Bsp143I GATC 9 cut(s) 139, 405, 607, 639, 649, 738, 1521, 1547, 1640
Bsp19I CCATGG 1 cut(s) 1468
BspACI CCGC 4 cut(s) 219, 393, 726, 1388
BspANI GGCC 5 cut(s) 431, 900, 1100, 1233, 1620
BspCNI CTCAG 1 cut(s) 699
BspFNI CGCG 1 cut(s) 221
BspLI GGNNCC 4 cut(s) 197, 386, 670, 1576
BspMI ACCTGC 1 cut(s) 1599
BspOI GCTAGC 1 cut(s) 1650
BspPI GGATC 4 cut(s) 615, 746, 1529, 1555
BspT107I GGYRCC 2 cut(s) 195, 668
BspTNI GGTCTC 2 cut(s) 813, 1061
BsrDI GCAATG 1 cut(s) 146
BsrFI RCCGGY 1 cut(s) 1404
BsrI ACTGG 2 cut(s) 545, 932
BssAI RCCGGY 1 cut(s) 1404
BssECI CCNNGG 5 cut(s) 388, 672, 753, 1468, 1571
BssMI GATC 9 cut(s) 139, 405, 607, 639, 649, 738, 1521, 1547, 1640
BssNI GRCGYC 1 cut(s) 332
BssT1I CCWWGG 3 cut(s) 388, 753, 1468
Bst2UI CCWGG 2 cut(s) 1359, 1572
Bst4CI ACNGT 4 cut(s) 326, 437, 1072, 1447
Bst6I CTCTTC 1 cut(s) 754
BstACI GRCGYC 1 cut(s) 332
BstAPI GCANNNNNTGC 3 cut(s) 722, 967, 1037
BstBAI YACGTR 3 cut(s) 128, 827, 1535
BstC8I GCNNGC 2 cut(s) 336, 1648
BstDEI CTNAG 3 cut(s) 478, 707, 887
BstDSI CCRYGG 2 cut(s) 672, 1468
BstENI CCTNNNNNAGG 2 cut(s) 63, 802
BstF5I GGATG 4 cut(s) 694, 1211, 1418, 1481
BstFNI CGCG 1 cut(s) 221
BstKTI GATC 9 cut(s) 142, 408, 610, 642, 652, 741, 1524, 1550, 1643
BstMAI GTCTC 4 cut(s) 813, 1061, 1078, 1434
BstMBI GATC 9 cut(s) 139, 405, 607, 639, 649, 738, 1521, 1547, 1640
BstMCI CGRYCG 1 cut(s) 652
BstMWI GCNNNNNNNGC 4 cut(s) 722, 967, 1037, 1265
BstNI CCWGG 2 cut(s) 1359, 1572
BstSCI CCNGG 4 cut(s) 1076, 1357, 1509, 1570
BstSLI GKGCMC 3 cut(s) 188, 200, 1158
BstSNI TACGTA 1 cut(s) 128
BstUI CGCG 1 cut(s) 221
BstV1I GCAGC 1 cut(s) 218
BstV2I GAAGAC 2 cut(s) 43, 667
BstX2I RGATCY 1 cut(s) 1547
BstYI RGATCY 1 cut(s) 1547
BsuRI GGCC 5 cut(s) 431, 900, 1100, 1233, 1620
BtgI CCRYGG 2 cut(s) 672, 1468
BtsCI GGATG 4 cut(s) 694, 1211, 1418, 1481
BtsIMutI CAGTG 3 cut(s) 442, 538, 789
BveI ACCTGC 1 cut(s) 1599
Cac8I GCNNGC 2 cut(s) 336, 1648
Cfr10I RCCGGY 1 cut(s) 1404
Cfr13I GGNCC 6 cut(s) 385, 429, 899, 1232, 1385, 1575
CseI GACGC 1 cut(s) 340
Csp6I GTAC 3 cut(s) 40, 125, 636
CspAI ACCGGT 1 cut(s) 1404
CviAII CATG 7 cut(s) 310, 472, 665, 722, 1132, 1469, 1516
CviQI GTAC 3 cut(s) 40, 125, 636
DdeI CTNAG 3 cut(s) 478, 707, 887
DpnI GATC 9 cut(s) 141, 407, 609, 641, 651, 740, 1523, 1549, 1642
DpnII GATC 9 cut(s) 139, 405, 607, 639, 649, 738, 1521, 1547, 1640
Eam1104I CTCTTC 1 cut(s) 754
EarI CTCTTC 1 cut(s) 754
Eco105I TACGTA 1 cut(s) 128
Eco130I CCWWGG 3 cut(s) 388, 753, 1468
Eco31I GGTCTC 2 cut(s) 813, 1061
Eco47I GGWCC 3 cut(s) 385, 1385, 1575
EcoNI CCTNNNNNAGG 2 cut(s) 63, 802
EcoO109I RGGNCCY 1 cut(s) 385
EcoRI GAATTC 1 cut(s) 440
EcoRII CCWGG 2 cut(s) 1357, 1570
EcoT14I CCWWGG 3 cut(s) 388, 753, 1468
EcoT22I ATGCAT 1 cut(s) 565
ErhI CCWWGG 3 cut(s) 388, 753, 1468
Esp3I CGTCTC 1 cut(s) 1078
FaeI CATG 7 cut(s) 313, 475, 668, 725, 1135, 1472, 1519
FalI AAGNNNNNCTT 2 cut(s) 1578, 1610
FaqI GGGAC 1 cut(s) 1588
FatI CATG 7 cut(s) 309, 471, 664, 721, 1131, 1468, 1515
FauI CCCGC 1 cut(s) 386
FauNDI CATATG 1 cut(s) 493
FbaI TGATCA 1 cut(s) 405
Fnu4HI GCNGC 2 cut(s) 207, 726
FokI GGATG 4 cut(s) 681, 1198, 1405, 1488
Fsp4HI GCNGC 2 cut(s) 207, 726
FspBI CTAG 6 cut(s) 24, 242, 389, 515, 1016, 1647
GluI GCNGC 2 cut(s) 207, 726
GsaI CCCAGC 1 cut(s) 294
HaeIII GGCC 5 cut(s) 431, 900, 1100, 1233, 1620
HapII CCGG 3 cut(s) 1077, 1405, 1510
HgaI GACGC 1 cut(s) 340
Hin1I GRCGYC 1 cut(s) 332
Hin1II CATG 7 cut(s) 313, 475, 668, 725, 1135, 1472, 1519
HinfI GANTC 5 cut(s) 295, 780, 794, 868, 1345
HpaII CCGG 3 cut(s) 1077, 1405, 1510
HphI GGTGA 4 cut(s) 848, 932, 1319, 1560
Hpy166II GTNNAC 3 cut(s) 73, 348, 940
Hpy188I TCNGA 5 cut(s) 355, 528, 1012, 1411, 1487
Hpy188III TCNNGA 6 cut(s) 101, 448, 456, 587, 1349, 1439
Hpy8I GTNNAC 3 cut(s) 73, 348, 940
Hpy99I CGWCG 1 cut(s) 1487
HpyAV CCTTC 3 cut(s) 99, 1032, 1631
HpyCH4III ACNGT 4 cut(s) 326, 437, 1072, 1447
HpyCH4IV ACGT 6 cut(s) 127, 276, 826, 954, 990, 1534
HpyF10VI GCNNNNNNNGC 4 cut(s) 722, 967, 1037, 1265
HpyF3I CTNAG 3 cut(s) 478, 707, 887
HpySE526I ACGT 6 cut(s) 127, 276, 826, 954, 990, 1534
Hsp92I GRCGYC 1 cut(s) 332
Hsp92II CATG 7 cut(s) 313, 475, 668, 725, 1135, 1472, 1519
Ksp22I TGATCA 1 cut(s) 405
Kzo9I GATC 9 cut(s) 139, 405, 607, 639, 649, 738, 1521, 1547, 1640
LmnI GCTCC 1 cut(s) 240
Lsp1109I GCAGC 1 cut(s) 218
LweI GCATC 4 cut(s) 550, 767, 1427, 1466
MaeI CTAG 6 cut(s) 24, 242, 389, 515, 1016, 1647
MaeII ACGT 6 cut(s) 127, 276, 826, 954, 990, 1534
MaeIII GTNAC 5 cut(s) 128, 284, 971, 991, 1162
MalI GATC 9 cut(s) 141, 407, 609, 641, 651, 740, 1523, 1549, 1642
MboI GATC 9 cut(s) 139, 405, 607, 639, 649, 738, 1521, 1547, 1640
MboII GAAGA 8 cut(s) 43, 329, 368, 669, 672, 741, 1376, 1646
MfeI CAATTG 2 cut(s) 15, 861
MflI RGATCY 1 cut(s) 1547
MhlI GDGCHC 4 cut(s) 188, 200, 237, 1158
MlyI GAGTC 2 cut(s) 877, 1339
MmeI TCCRAC 3 cut(s) 157, 1145, 1465
Mph1103I ATGCAT 1 cut(s) 565
MseI TTAA 6 cut(s) 462, 924, 1200, 1316, 1341, 1374
MslI CAYNNNNRTG 4 cut(s) 496, 515, 1467, 1595
MspI CCGG 3 cut(s) 1077, 1405, 1510
MspR9I CCNGG 4 cut(s) 1078, 1359, 1511, 1572
MunI CAATTG 2 cut(s) 15, 861
MvaI CCWGG 2 cut(s) 1359, 1572
MvnI CGCG 1 cut(s) 221
MwoI GCNNNNNNNGC 4 cut(s) 722, 967, 1037, 1265
NciI CCSGG 2 cut(s) 1078, 1511
NcoI CCATGG 1 cut(s) 1468
NdeI CATATG 1 cut(s) 493
NdeII GATC 9 cut(s) 139, 405, 607, 639, 649, 738, 1521, 1547, 1640
NheI GCTAGC 1 cut(s) 1646
NlaIII CATG 7 cut(s) 313, 475, 668, 725, 1135, 1472, 1519
NlaIV GGNNCC 4 cut(s) 197, 386, 670, 1576
NmuCI GTSAC 1 cut(s) 971
NsiI ATGCAT 1 cut(s) 565
OliI CACNNNNGTG 1 cut(s) 515
PfeI GAWTC 3 cut(s) 295, 780, 794
PflMI CCANNNNNTGG 2 cut(s) 593, 1458
PinAI ACCGGT 1 cut(s) 1404
PkrI GCNGC 2 cut(s) 208, 727
Ple19I CGATCG 1 cut(s) 652
PleI GAGTC 2 cut(s) 876, 1339
PpsI GAGTC 2 cut(s) 876, 1339
Ppu21I YACGTR 3 cut(s) 128, 827, 1535
PpuMI RGGWCCY 1 cut(s) 385
Psp5II RGGWCCY 1 cut(s) 385
Psp6I CCWGG 2 cut(s) 1357, 1570
PspFI CCCAGC 1 cut(s) 290
PspGI CCWGG 2 cut(s) 1357, 1570
PspN4I GGNNCC 4 cut(s) 197, 386, 670, 1576
PspPI GGNCC 6 cut(s) 385, 429, 899, 1232, 1385, 1575
PspPPI RGGWCCY 1 cut(s) 385
PsuI RGATCY 1 cut(s) 1547
PvuI CGATCG 1 cut(s) 652
RsaI GTAC 3 cut(s) 41, 126, 637
RsaNI GTAC 3 cut(s) 40, 125, 636
RseI CAYNNNNRTG 4 cut(s) 496, 515, 1467, 1595
SaqAI TTAA 6 cut(s) 462, 924, 1200, 1316, 1341, 1374
SatI GCNGC 2 cut(s) 207, 726
Sau3AI GATC 9 cut(s) 139, 405, 607, 639, 649, 738, 1521, 1547, 1640
Sau96I GGNCC 6 cut(s) 385, 429, 899, 1232, 1385, 1575
SchI GAGTC 2 cut(s) 877, 1339
ScrFI CCNGG 4 cut(s) 1078, 1359, 1511, 1572
SduI GDGCHC 4 cut(s) 188, 200, 237, 1158
SfaNI GCATC 4 cut(s) 550, 767, 1427, 1466
SinI GGWCC 3 cut(s) 385, 1385, 1575
SmiMI CAYNNNNRTG 4 cut(s) 496, 515, 1467, 1595
SmlI CTYRAG 4 cut(s) 424, 446, 1347, 1584
SmoI CTYRAG 4 cut(s) 424, 446, 1347, 1584
SnaBI TACGTA 1 cut(s) 128
SsiI CCGC 4 cut(s) 219, 393, 726, 1388
SspI AATATT 1 cut(s) 412
SspMI CTAG 6 cut(s) 24, 242, 389, 515, 1016, 1647
StyD4I CCNGG 4 cut(s) 1076, 1357, 1509, 1570
StyI CCWWGG 3 cut(s) 388, 753, 1468
TaaI ACNGT 4 cut(s) 326, 437, 1072, 1447
TaiI ACGT 6 cut(s) 130, 279, 829, 957, 993, 1537
TaqI TCGA 3 cut(s) 1438, 1542, 1624
TatI WGTACW 1 cut(s) 39
TauI GCSGC 1 cut(s) 728
TfiI GAWTC 3 cut(s) 295, 780, 794
Tru1I TTAA 6 cut(s) 462, 924, 1200, 1316, 1341, 1374
Tru9I TTAA 6 cut(s) 462, 924, 1200, 1316, 1341, 1374
TscAI CASTG 3 cut(s) 442, 545, 796
TseFI GTSAC 1 cut(s) 971
TseI GCWGC 1 cut(s) 206
Tsp45I GTSAC 1 cut(s) 971
TspDTI ATGAA 7 cut(s) 298, 480, 488, 510, 710, 906, 1415
TspGWI ACGGA 2 cut(s) 220, 512
TspRI CASTG 3 cut(s) 442, 545, 796
Van91I CCANNNNNTGG 2 cut(s) 593, 1458
VpaK11BI GGWCC 3 cut(s) 385, 1385, 1575
XagI CCTNNNNNAGG 2 cut(s) 63, 802
XapI RAATTY 6 cut(s) 119, 304, 440, 529, 1141, 1244
XmaJI CCTAGG 1 cut(s) 388
XspI CTAG 6 cut(s) 24, 242, 389, 515, 1016, 1647
Zsp2I ATGCAT 1 cut(s) 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.