(R)-mandelonitrile lyase
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 372 |
| AccI | GTMKAC | 1 cut(s) | 1158 |
| AccIII | TCCGGA | 1 cut(s) | 475 |
| AciI | CCGC | 2 cut(s) | 32, 409 |
| AclI | AACGTT | 1 cut(s) | 843 |
| AclWI | GGATC | 3 cut(s) | 211, 278, 635 |
| AcoI | YGGCCR | 1 cut(s) | 1116 |
| AcsI | RAATTY | 1 cut(s) | 1201 |
| AfaI | GTAC | 3 cut(s) | 714, 1023, 1213 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 482, 845 |
| AgsI | TTSAA | 6 cut(s) | 350, 566, 915, 1099, 1183, 1277 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 694 |
| AjiI | CACGTC | 1 cut(s) | 1072 |
| AjnI | CCWGG | 2 cut(s) | 505, 1024 |
| AluBI | AGCT | 4 cut(s) | 224, 512, 560, 1095 |
| AluI | AGCT | 4 cut(s) | 224, 512, 560, 1095 |
| Alw26I | GTCTC | 2 cut(s) | 813, 1200 |
| AlwI | GGATC | 3 cut(s) | 211, 278, 635 |
| Aor13HI | TCCGGA | 1 cut(s) | 475 |
| AoxI | GGCC | 2 cut(s) | 1082, 1116 |
| ApeKI | GCWGC | 1 cut(s) | 1131 |
| ApoI | RAATTY | 1 cut(s) | 1201 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 249, 501 |
| AspLEI | GCGC | 1 cut(s) | 15 |
| AspS9I | GGNCC | 3 cut(s) | 1028, 1082, 1235 |
| AsuC2I | CCSGG | 2 cut(s) | 413, 821 |
| AsuHPI | GGTGA | 1 cut(s) | 226 |
| AsuII | TTCGAA | 1 cut(s) | 1016 |
| AvaII | GGWCC | 2 cut(s) | 1028, 1235 |
| BbvI | GCAGC | 1 cut(s) | 1118 |
| BccI | CCATC | 2 cut(s) | 203, 1246 |
| BciT130I | CCWGG | 2 cut(s) | 507, 1026 |
| BclI | TGATCA | 1 cut(s) | 291 |
| BcnI | CCSGG | 2 cut(s) | 413, 821 |
| BcoDI | GTCTC | 2 cut(s) | 813, 1200 |
| BfaI | CTAG | 3 cut(s) | 35, 128, 147 |
| BisI | GCNGC | 1 cut(s) | 1132 |
| BlsI | GCNGC | 1 cut(s) | 1133 |
| Bme1390I | CCNGG | 4 cut(s) | 413, 507, 821, 1026 |
| Bme18I | GGWCC | 2 cut(s) | 1028, 1235 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 694 |
| BmgBI | CACGTC | 1 cut(s) | 1072 |
| BmgT120I | GGNCC | 3 cut(s) | 1028, 1082, 1235 |
| BmiI | GGNNCC | 1 cut(s) | 1030 |
| BmrFI | CCNGG | 4 cut(s) | 413, 507, 821, 1026 |
| BmsI | GCATC | 2 cut(s) | 644, 1255 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 947, 979 |
| BpmI | CTGGAG | 1 cut(s) | 295 |
| Bpu14I | TTCGAA | 1 cut(s) | 1016 |
| BpuEI | CTTGAG | 1 cut(s) | 926 |
| BpuMI | CCSGG | 2 cut(s) | 413, 821 |
| BsaAI | YACGTR | 1 cut(s) | 716 |
| BsaI | GGTCTC | 1 cut(s) | 1200 |
| BsaWI | WCCGGW | 2 cut(s) | 475, 1050 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 501, 531 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 482, 845 |
| Bse3DI | GCAATG | 1 cut(s) | 997 |
| BseAI | TCCGGA | 1 cut(s) | 475 |
| BseBI | CCWGG | 2 cut(s) | 507, 1026 |
| BseGI | GGATG | 1 cut(s) | 1111 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 482, 845 |
| BseMI | GCAATG | 1 cut(s) | 997 |
| BseMII | CTCAG | 3 cut(s) | 252, 790, 1136 |
| BseX3I | CGGCCG | 1 cut(s) | 1116 |
| BseXI | GCAGC | 1 cut(s) | 1118 |
| BseYI | CCCAGC | 1 cut(s) | 220 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1119 |
| BshFI | GGCC | 2 cut(s) | 1084, 1118 |
| BsiEI | CGRYCG | 1 cut(s) | 1119 |
| BsiSI | CCGG | 5 cut(s) | 413, 476, 821, 1051, 1115 |
| BslFI | GGGAC | 1 cut(s) | 1155 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 482, 845 |
| BsmAI | GTCTC | 2 cut(s) | 813, 1200 |
| BsmBI | CGTCTC | 1 cut(s) | 813 |
| BsmFI | GGGAC | 1 cut(s) | 1155 |
| BsnI | GGCC | 2 cut(s) | 1084, 1118 |
| Bso31I | GGTCTC | 1 cut(s) | 1200 |
| Bsp119I | TTCGAA | 1 cut(s) | 1016 |
| Bsp13I | TCCGGA | 1 cut(s) | 475 |
| Bsp143I | GATC | 5 cut(s) | 25, 216, 270, 291, 627 |
| BspACI | CCGC | 2 cut(s) | 32, 409 |
| BspANI | GGCC | 2 cut(s) | 1084, 1118 |
| BspCNI | CTCAG | 3 cut(s) | 251, 789, 1137 |
| BspEI | TCCGGA | 1 cut(s) | 475 |
| BspLI | GGNNCC | 1 cut(s) | 1030 |
| BspPI | GGATC | 3 cut(s) | 211, 278, 635 |
| BspT104I | TTCGAA | 1 cut(s) | 1016 |
| BspTNI | GGTCTC | 1 cut(s) | 1200 |
| BsrDI | GCAATG | 1 cut(s) | 997 |
| BssMI | GATC | 5 cut(s) | 25, 216, 270, 291, 627 |
| Bst2UI | CCWGG | 2 cut(s) | 507, 1026 |
| Bst4CI | ACNGT | 5 cut(s) | 403, 539, 619, 680, 852 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 611, 1010, 1265 |
| BstBAI | YACGTR | 1 cut(s) | 716 |
| BstBI | TTCGAA | 1 cut(s) | 1016 |
| BstC8I | GCNNGC | 2 cut(s) | 79, 1129 |
| BstDEI | CTNAG | 3 cut(s) | 238, 776, 1145 |
| BstF5I | GGATG | 1 cut(s) | 1111 |
| BstHHI | GCGC | 1 cut(s) | 15 |
| BstKTI | GATC | 5 cut(s) | 28, 219, 273, 294, 630 |
| BstMAI | GTCTC | 2 cut(s) | 813, 1200 |
| BstMBI | GATC | 5 cut(s) | 25, 216, 270, 291, 627 |
| BstMCI | CGRYCG | 1 cut(s) | 1119 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 611, 1010, 1115, 1124, 1265 |
| BstNI | CCWGG | 2 cut(s) | 507, 1026 |
| BstSCI | CCNGG | 4 cut(s) | 411, 505, 819, 1024 |
| BstV1I | GCAGC | 1 cut(s) | 1118 |
| BstX2I | RGATCY | 2 cut(s) | 216, 270 |
| BstYI | RGATCY | 2 cut(s) | 216, 270 |
| BstZI | CGGCCG | 1 cut(s) | 1116 |
| BsuRI | GGCC | 2 cut(s) | 1084, 1118 |
| BtrI | CACGTC | 1 cut(s) | 1072 |
| BtsCI | GGATG | 1 cut(s) | 1111 |
| BtsI | GCAGTG | 1 cut(s) | 145 |
| BtsIMutI | CAGTG | 3 cut(s) | 145, 685, 784 |
| Cac8I | GCNNGC | 2 cut(s) | 79, 1129 |
| CfoI | GCGC | 1 cut(s) | 15 |
| Cfr13I | GGNCC | 3 cut(s) | 1028, 1082, 1235 |
| Csp6I | GTAC | 3 cut(s) | 713, 1022, 1212 |
| CviAII | CATG | 3 cut(s) | 196, 554, 611 |
| CviQI | GTAC | 3 cut(s) | 713, 1022, 1212 |
| DdeI | CTNAG | 3 cut(s) | 238, 776, 1145 |
| DpnI | GATC | 5 cut(s) | 27, 218, 272, 293, 629 |
| DpnII | GATC | 5 cut(s) | 25, 216, 270, 291, 627 |
| DriI | GACNNNNNGTC | 1 cut(s) | 694 |
| EaeI | YGGCCR | 1 cut(s) | 1116 |
| EagI | CGGCCG | 1 cut(s) | 1116 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 694 |
| EciI | GGCGGA | 1 cut(s) | 398 |
| EclXI | CGGCCG | 1 cut(s) | 1116 |
| Eco31I | GGTCTC | 1 cut(s) | 1200 |
| Eco47I | GGWCC | 2 cut(s) | 1028, 1235 |
| Eco52I | CGGCCG | 1 cut(s) | 1116 |
| EcoRII | CCWGG | 2 cut(s) | 505, 1024 |
| Esp3I | CGTCTC | 1 cut(s) | 813 |
| FaeI | CATG | 3 cut(s) | 199, 557, 614 |
| FaqI | GGGAC | 1 cut(s) | 1155 |
| FatI | CATG | 3 cut(s) | 195, 553, 610 |
| FauNDI | CATATG | 1 cut(s) | 1039 |
| FbaI | TGATCA | 1 cut(s) | 291 |
| FblI | GTMKAC | 1 cut(s) | 1158 |
| Fnu4HI | GCNGC | 1 cut(s) | 1132 |
| FokI | GGATG | 1 cut(s) | 1098 |
| Fsp4HI | GCNGC | 1 cut(s) | 1132 |
| FspBI | CTAG | 3 cut(s) | 35, 128, 147 |
| GlaI | GCGC | 1 cut(s) | 14 |
| GluI | GCNGC | 1 cut(s) | 1132 |
| GsaI | CCCAGC | 1 cut(s) | 224 |
| GsuI | CTGGAG | 1 cut(s) | 295 |
| HaeIII | GGCC | 2 cut(s) | 1084, 1118 |
| HapII | CCGG | 5 cut(s) | 413, 476, 821, 1051, 1115 |
| HhaI | GCGC | 1 cut(s) | 15 |
| Hin1II | CATG | 3 cut(s) | 199, 557, 614 |
| Hin6I | GCGC | 1 cut(s) | 13 |
| HinP1I | GCGC | 1 cut(s) | 13 |
| HinfI | GANTC | 1 cut(s) | 683 |
| HpaII | CCGG | 5 cut(s) | 413, 476, 821, 1051, 1115 |
| HphI | GGTGA | 1 cut(s) | 226 |
| Hpy166II | GTNNAC | 3 cut(s) | 183, 234, 1159 |
| Hpy188I | TCNGA | 5 cut(s) | 106, 241, 688, 1069, 1286 |
| Hpy188III | TCNNGA | 5 cut(s) | 128, 312, 342, 476, 651 |
| Hpy8I | GTNNAC | 3 cut(s) | 183, 234, 1159 |
| HpyAV | CCTTC | 4 cut(s) | 239, 540, 921, 1211 |
| HpyCH4III | ACNGT | 5 cut(s) | 403, 539, 619, 680, 852 |
| HpyCH4IV | ACGT | 3 cut(s) | 715, 843, 1071 |
| HpyCH4V | TGCA | 7 cut(s) | 152, 199, 260, 598, 605, 1057, 1109 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 611, 1010, 1115, 1124, 1265 |
| HpyF3I | CTNAG | 3 cut(s) | 238, 776, 1145 |
| HpySE526I | ACGT | 3 cut(s) | 715, 843, 1071 |
| Hsp92II | CATG | 3 cut(s) | 199, 557, 614 |
| HspAI | GCGC | 1 cut(s) | 13 |
| Kpn2I | TCCGGA | 1 cut(s) | 475 |
| Ksp22I | TGATCA | 1 cut(s) | 291 |
| Kzo9I | GATC | 5 cut(s) | 25, 216, 270, 291, 627 |
| LmnI | GCTCC | 3 cut(s) | 509, 557, 1273 |
| Lsp1109I | GCAGC | 1 cut(s) | 1118 |
| LweI | GCATC | 2 cut(s) | 644, 1255 |
| MaeI | CTAG | 3 cut(s) | 35, 128, 147 |
| MaeII | ACGT | 3 cut(s) | 715, 843, 1071 |
| MaeIII | GTNAC | 3 cut(s) | 469, 696, 994 |
| MalI | GATC | 5 cut(s) | 27, 218, 272, 293, 629 |
| MboI | GATC | 5 cut(s) | 25, 216, 270, 291, 627 |
| MboII | GAAGA | 7 cut(s) | 50, 53, 185, 215, 254, 630, 1056 |
| MflI | RGATCY | 2 cut(s) | 216, 270 |
| MluCI | AATT | 8 cut(s) | 345, 490, 669, 874, 924, 933, 982, 1201 |
| MmeI | TCCRAC | 1 cut(s) | 826 |
| MroI | TCCGGA | 1 cut(s) | 475 |
| MroXI | GAANNNNTTC | 2 cut(s) | 249, 501 |
| MseI | TTAA | 8 cut(s) | 417, 489, 573, 813, 882, 1137, 1166, 1224 |
| MspI | CCGG | 5 cut(s) | 413, 476, 821, 1051, 1115 |
| MspR9I | CCNGG | 4 cut(s) | 413, 507, 821, 1026 |
| MvaI | CCWGG | 2 cut(s) | 507, 1026 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 611, 1010, 1115, 1124, 1265 |
| NciI | CCSGG | 2 cut(s) | 413, 821 |
| NdeI | CATATG | 1 cut(s) | 1039 |
| NdeII | GATC | 5 cut(s) | 25, 216, 270, 291, 627 |
| NlaIII | CATG | 3 cut(s) | 199, 557, 614 |
| NlaIV | GGNNCC | 1 cut(s) | 1030 |
| NmuCI | GTSAC | 2 cut(s) | 696, 994 |
| NspV | TTCGAA | 1 cut(s) | 1016 |
| PdmI | GAANNNNTTC | 2 cut(s) | 249, 501 |
| PfeI | GAWTC | 1 cut(s) | 683 |
| PfoI | TCCNGGA | 1 cut(s) | 505 |
| PkrI | GCNGC | 1 cut(s) | 1133 |
| Ppu21I | YACGTR | 1 cut(s) | 716 |
| PsiI | TTATAA | 1 cut(s) | 372 |
| Psp1406I | AACGTT | 1 cut(s) | 843 |
| Psp6I | CCWGG | 2 cut(s) | 505, 1024 |
| PspFI | CCCAGC | 1 cut(s) | 220 |
| PspGI | CCWGG | 2 cut(s) | 505, 1024 |
| PspN4I | GGNNCC | 1 cut(s) | 1030 |
| PspPI | GGNCC | 3 cut(s) | 1028, 1082, 1235 |
| PsuI | RGATCY | 2 cut(s) | 216, 270 |
| RsaI | GTAC | 3 cut(s) | 714, 1023, 1213 |
| RsaNI | GTAC | 3 cut(s) | 713, 1022, 1212 |
| SaqAI | TTAA | 8 cut(s) | 417, 489, 573, 813, 882, 1137, 1166, 1224 |
| SatI | GCNGC | 1 cut(s) | 1132 |
| Sau3AI | GATC | 5 cut(s) | 25, 216, 270, 291, 627 |
| Sau96I | GGNCC | 3 cut(s) | 1028, 1082, 1235 |
| ScrFI | CCNGG | 4 cut(s) | 413, 507, 821, 1026 |
| SfaNI | GCATC | 2 cut(s) | 644, 1255 |
| SfuI | TTCGAA | 1 cut(s) | 1016 |
| SinI | GGWCC | 2 cut(s) | 1028, 1235 |
| SmlI | CTYRAG | 1 cut(s) | 905 |
| SmoI | CTYRAG | 1 cut(s) | 905 |
| Sse9I | AATT | 8 cut(s) | 345, 490, 669, 874, 924, 933, 982, 1201 |
| SsiI | CCGC | 2 cut(s) | 32, 409 |
| SspI | AATATT | 1 cut(s) | 1009 |
| SspMI | CTAG | 3 cut(s) | 35, 128, 147 |
| StyD4I | CCNGG | 4 cut(s) | 411, 505, 819, 1024 |
| TaaI | ACNGT | 5 cut(s) | 403, 539, 619, 680, 852 |
| TaiI | ACGT | 3 cut(s) | 718, 846, 1074 |
| TaqI | TCGA | 5 cut(s) | 284, 390, 527, 861, 1016 |
| TasI | AATT | 8 cut(s) | 345, 490, 669, 874, 924, 933, 982, 1201 |
| TfiI | GAWTC | 1 cut(s) | 683 |
| Tru1I | TTAA | 8 cut(s) | 417, 489, 573, 813, 882, 1137, 1166, 1224 |
| Tru9I | TTAA | 8 cut(s) | 417, 489, 573, 813, 882, 1137, 1166, 1224 |
| TscAI | CASTG | 3 cut(s) | 145, 685, 784 |
| TseFI | GTSAC | 2 cut(s) | 696, 994 |
| TseI | GCWGC | 1 cut(s) | 1131 |
| Tsp45I | GTSAC | 2 cut(s) | 696, 994 |
| TspDTI | ATGAA | 3 cut(s) | 17, 599, 868 |
| TspGWI | ACGGA | 1 cut(s) | 1229 |
| TspRI | CASTG | 3 cut(s) | 145, 685, 784 |
| VpaK11BI | GGWCC | 2 cut(s) | 1028, 1235 |
| XapI | RAATTY | 1 cut(s) | 1201 |
| XbaI | TCTAGA | 1 cut(s) | 127 |
| XmiI | GTMKAC | 1 cut(s) | 1158 |
| XmnI | GAANNNNTTC | 2 cut(s) | 249, 501 |
| XspI | CTAG | 3 cut(s) | 35, 128, 147 |