(R)-mandelonitrile lyase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 201 |
| Acc36I | ACCTGC | 1 cut(s) | 888 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 750 |
| AccIII | TCCGGA | 1 cut(s) | 304 |
| AclWI | GGATC | 3 cut(s) | 110, 464, 821 |
| AcsI | RAATTY | 1 cut(s) | 643 |
| AfaI | GTAC | 3 cut(s) | 80, 543, 655 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 311, 633, 750 |
| AflII | CTTAAG | 1 cut(s) | 911 |
| AflIII | ACRYGT | 1 cut(s) | 826 |
| AgsI | TTSAA | 5 cut(s) | 56, 179, 395, 719, 850 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 523 |
| AjnI | CCWGG | 2 cut(s) | 334, 873 |
| AluBI | AGCT | 3 cut(s) | 22, 341, 389 |
| AluI | AGCT | 3 cut(s) | 22, 341, 389 |
| Alw26I | GTCTC | 1 cut(s) | 642 |
| AlwI | GGATC | 3 cut(s) | 110, 464, 821 |
| Aor13HI | TCCGGA | 1 cut(s) | 304 |
| AoxI | GGCC | 2 cut(s) | 876, 907 |
| ApeKI | GCWGC | 1 cut(s) | 919 |
| ApoI | RAATTY | 1 cut(s) | 643 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 330 |
| AspS9I | GGNCC | 2 cut(s) | 677, 864 |
| AsuC2I | CCSGG | 2 cut(s) | 242, 861 |
| AsuHPI | GGTGA | 2 cut(s) | 122, 849 |
| AvaII | GGWCC | 2 cut(s) | 677, 864 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 62, 94 |
| BbsI | GAAGAC | 1 cut(s) | 592 |
| BbvI | GCAGC | 1 cut(s) | 906 |
| BccI | CCATC | 2 cut(s) | 688, 830 |
| BciT130I | CCWGG | 2 cut(s) | 336, 875 |
| BcnI | CCSGG | 2 cut(s) | 242, 861 |
| BcoDI | GTCTC | 1 cut(s) | 642 |
| BfmI | CTRYAG | 1 cut(s) | 735 |
| BfrI | CTTAAG | 1 cut(s) | 911 |
| BfuAI | ACCTGC | 1 cut(s) | 888 |
| BisI | GCNGC | 1 cut(s) | 920 |
| BlsI | GCNGC | 1 cut(s) | 921 |
| Bme1390I | CCNGG | 4 cut(s) | 242, 336, 861, 875 |
| Bme18I | GGWCC | 2 cut(s) | 677, 864 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 523 |
| BmgT120I | GGNCC | 2 cut(s) | 677, 864 |
| BmiI | GGNNCC | 2 cut(s) | 841, 865 |
| BmrFI | CCNGG | 4 cut(s) | 242, 336, 861, 875 |
| BmsI | GCATC | 3 cut(s) | 697, 758, 906 |
| BpiI | GAAGAC | 1 cut(s) | 592 |
| BpmI | CTGGAG | 1 cut(s) | 124 |
| BpuMI | CCSGG | 2 cut(s) | 242, 861 |
| BsaAI | YACGTR | 2 cut(s) | 545, 827 |
| BsaI | GGTCTC | 1 cut(s) | 642 |
| BsaJI | CCNNGG | 3 cut(s) | 760, 860, 873 |
| BsaWI | WCCGGW | 1 cut(s) | 304 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 330, 360 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 311, 633, 750 |
| BseAI | TCCGGA | 1 cut(s) | 304 |
| BseBI | CCWGG | 2 cut(s) | 336, 875 |
| BseDI | CCNNGG | 3 cut(s) | 760, 860, 873 |
| BseGI | GGATG | 1 cut(s) | 773 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 311, 633, 750 |
| BseMII | CTCAG | 1 cut(s) | 619 |
| BseXI | GCAGC | 1 cut(s) | 906 |
| BshFI | GGCC | 2 cut(s) | 878, 909 |
| BsiSI | CCGG | 3 cut(s) | 242, 305, 860 |
| BslFI | GGGAC | 1 cut(s) | 877 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 311, 633, 750 |
| BsmAI | GTCTC | 1 cut(s) | 642 |
| BsmFI | GGGAC | 1 cut(s) | 877 |
| BsnI | GGCC | 2 cut(s) | 878, 909 |
| Bso31I | GGTCTC | 1 cut(s) | 642 |
| Bsp13I | TCCGGA | 1 cut(s) | 304 |
| Bsp143I | GATC | 3 cut(s) | 102, 456, 813 |
| Bsp19I | CCATGG | 1 cut(s) | 760 |
| BspANI | GGCC | 2 cut(s) | 878, 909 |
| BspCNI | CTCAG | 1 cut(s) | 618 |
| BspEI | TCCGGA | 1 cut(s) | 304 |
| BspLI | GGNNCC | 2 cut(s) | 841, 865 |
| BspMI | ACCTGC | 1 cut(s) | 888 |
| BspPI | GGATC | 3 cut(s) | 110, 464, 821 |
| BspTI | CTTAAG | 1 cut(s) | 911 |
| BspTNI | GGTCTC | 1 cut(s) | 642 |
| BssECI | CCNNGG | 3 cut(s) | 760, 860, 873 |
| BssMI | GATC | 3 cut(s) | 102, 456, 813 |
| BssT1I | CCWWGG | 1 cut(s) | 760 |
| Bst2UI | CCWGG | 2 cut(s) | 336, 875 |
| Bst4CI | ACNGT | 4 cut(s) | 232, 368, 448, 739 |
| BstAFI | CTTAAG | 1 cut(s) | 911 |
| BstBAI | YACGTR | 2 cut(s) | 545, 827 |
| BstDEI | CTNAG | 1 cut(s) | 605 |
| BstDSI | CCRYGG | 1 cut(s) | 760 |
| BstF5I | GGATG | 1 cut(s) | 773 |
| BstKTI | GATC | 3 cut(s) | 105, 459, 816 |
| BstMAI | GTCTC | 1 cut(s) | 642 |
| BstMBI | GATC | 3 cut(s) | 102, 456, 813 |
| BstNI | CCWGG | 2 cut(s) | 336, 875 |
| BstSCI | CCNGG | 4 cut(s) | 240, 334, 859, 873 |
| BstSFI | CTRYAG | 1 cut(s) | 735 |
| BstV1I | GCAGC | 1 cut(s) | 906 |
| BstV2I | GAAGAC | 1 cut(s) | 592 |
| BstX2I | RGATCY | 1 cut(s) | 102 |
| BstYI | RGATCY | 1 cut(s) | 102 |
| BsuRI | GGCC | 2 cut(s) | 878, 909 |
| BtgI | CCRYGG | 1 cut(s) | 760 |
| BtsCI | GGATG | 1 cut(s) | 773 |
| BtsIMutI | CAGTG | 1 cut(s) | 613 |
| BveI | ACCTGC | 1 cut(s) | 888 |
| Cfr13I | GGNCC | 2 cut(s) | 677, 864 |
| Csp6I | GTAC | 3 cut(s) | 79, 542, 654 |
| CviAII | CATG | 3 cut(s) | 383, 440, 761 |
| CviQI | GTAC | 3 cut(s) | 79, 542, 654 |
| DdeI | CTNAG | 1 cut(s) | 605 |
| DpnI | GATC | 3 cut(s) | 104, 458, 815 |
| DpnII | GATC | 3 cut(s) | 102, 456, 813 |
| DriI | GACNNNNNGTC | 1 cut(s) | 523 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 523 |
| Eco130I | CCWWGG | 1 cut(s) | 760 |
| Eco31I | GGTCTC | 1 cut(s) | 642 |
| Eco32I | GATATC | 1 cut(s) | 37 |
| Eco47I | GGWCC | 2 cut(s) | 677, 864 |
| EcoRII | CCWGG | 2 cut(s) | 334, 873 |
| EcoRV | GATATC | 1 cut(s) | 37 |
| EcoT14I | CCWWGG | 1 cut(s) | 760 |
| ErhI | CCWWGG | 1 cut(s) | 760 |
| FaeI | CATG | 3 cut(s) | 386, 443, 764 |
| FaqI | GGGAC | 1 cut(s) | 877 |
| FatI | CATG | 3 cut(s) | 382, 439, 760 |
| Fnu4HI | GCNGC | 1 cut(s) | 920 |
| FokI | GGATG | 1 cut(s) | 780 |
| Fsp4HI | GCNGC | 1 cut(s) | 920 |
| GluI | GCNGC | 1 cut(s) | 920 |
| GsuI | CTGGAG | 1 cut(s) | 124 |
| HaeIII | GGCC | 2 cut(s) | 878, 909 |
| HapII | CCGG | 3 cut(s) | 242, 305, 860 |
| Hin1II | CATG | 3 cut(s) | 386, 443, 764 |
| HinfI | GANTC | 4 cut(s) | 62, 113, 512, 804 |
| HpaII | CCGG | 3 cut(s) | 242, 305, 860 |
| HphI | GGTGA | 2 cut(s) | 122, 849 |
| Hpy166II | GTNNAC | 1 cut(s) | 79 |
| Hpy188I | TCNGA | 1 cut(s) | 517 |
| Hpy188III | TCNNGA | 3 cut(s) | 141, 171, 305 |
| Hpy8I | GTNNAC | 1 cut(s) | 79 |
| HpyAV | CCTTC | 6 cut(s) | 22, 50, 67, 369, 653, 733 |
| HpyCH4III | ACNGT | 4 cut(s) | 232, 368, 448, 739 |
| HpyCH4IV | ACGT | 3 cut(s) | 81, 544, 826 |
| HpyCH4V | TGCA | 4 cut(s) | 92, 427, 434, 922 |
| HpyF3I | CTNAG | 1 cut(s) | 605 |
| HpySE526I | ACGT | 3 cut(s) | 81, 544, 826 |
| Hsp92II | CATG | 3 cut(s) | 386, 443, 764 |
| Kpn2I | TCCGGA | 1 cut(s) | 304 |
| Kzo9I | GATC | 3 cut(s) | 102, 456, 813 |
| LmnI | GCTCC | 3 cut(s) | 338, 386, 715 |
| Lsp1109I | GCAGC | 1 cut(s) | 906 |
| LweI | GCATC | 3 cut(s) | 697, 758, 906 |
| MaeII | ACGT | 3 cut(s) | 81, 544, 826 |
| MaeIII | GTNAC | 2 cut(s) | 298, 525 |
| MalI | GATC | 3 cut(s) | 104, 458, 815 |
| MboI | GATC | 3 cut(s) | 102, 456, 813 |
| MboII | GAAGA | 3 cut(s) | 82, 597, 639 |
| MfeI | CAATTG | 1 cut(s) | 937 |
| MflI | RGATCY | 1 cut(s) | 102 |
| MluCI | AATT | 8 cut(s) | 40, 174, 319, 498, 643, 817, 850, 937 |
| MlyI | GAGTC | 3 cut(s) | 56, 122, 813 |
| MnlI | CCTC | 7 cut(s) | 89, 283, 372, 447, 516, 550, 600 |
| MroI | TCCGGA | 1 cut(s) | 304 |
| MroXI | GAANNNNTTC | 1 cut(s) | 330 |
| MseI | TTAA | 5 cut(s) | 246, 318, 402, 666, 912 |
| MslI | CAYNNNNRTG | 3 cut(s) | 759, 884, 887 |
| MspCI | CTTAAG | 1 cut(s) | 911 |
| MspI | CCGG | 3 cut(s) | 242, 305, 860 |
| MspR9I | CCNGG | 4 cut(s) | 242, 336, 861, 875 |
| MunI | CAATTG | 1 cut(s) | 937 |
| MvaI | CCWGG | 2 cut(s) | 336, 875 |
| NciI | CCSGG | 2 cut(s) | 242, 861 |
| NcoI | CCATGG | 1 cut(s) | 760 |
| NdeII | GATC | 3 cut(s) | 102, 456, 813 |
| NlaIII | CATG | 3 cut(s) | 386, 443, 764 |
| NlaIV | GGNNCC | 2 cut(s) | 841, 865 |
| NmuCI | GTSAC | 1 cut(s) | 525 |
| PdmI | GAANNNNTTC | 1 cut(s) | 330 |
| PfeI | GAWTC | 1 cut(s) | 512 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 750 |
| PfoI | TCCNGGA | 1 cut(s) | 334 |
| PkrI | GCNGC | 1 cut(s) | 921 |
| PleI | GAGTC | 3 cut(s) | 56, 121, 812 |
| PpsI | GAGTC | 3 cut(s) | 56, 121, 812 |
| Ppu21I | YACGTR | 2 cut(s) | 545, 827 |
| PsiI | TTATAA | 1 cut(s) | 201 |
| Psp6I | CCWGG | 2 cut(s) | 334, 873 |
| PspGI | CCWGG | 2 cut(s) | 334, 873 |
| PspN4I | GGNNCC | 2 cut(s) | 841, 865 |
| PspPI | GGNCC | 2 cut(s) | 677, 864 |
| PsuI | RGATCY | 1 cut(s) | 102 |
| RsaI | GTAC | 3 cut(s) | 80, 543, 655 |
| RsaNI | GTAC | 3 cut(s) | 79, 542, 654 |
| RseI | CAYNNNNRTG | 3 cut(s) | 759, 884, 887 |
| SaqAI | TTAA | 5 cut(s) | 246, 318, 402, 666, 912 |
| SatI | GCNGC | 1 cut(s) | 920 |
| Sau3AI | GATC | 3 cut(s) | 102, 456, 813 |
| Sau96I | GGNCC | 2 cut(s) | 677, 864 |
| SchI | GAGTC | 3 cut(s) | 56, 122, 813 |
| ScrFI | CCNGG | 4 cut(s) | 242, 336, 861, 875 |
| SfaNI | GCATC | 3 cut(s) | 697, 758, 906 |
| SfcI | CTRYAG | 1 cut(s) | 735 |
| SinI | GGWCC | 2 cut(s) | 677, 864 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 759, 884, 887 |
| SmlI | CTYRAG | 1 cut(s) | 911 |
| SmoI | CTYRAG | 1 cut(s) | 911 |
| Sse9I | AATT | 8 cut(s) | 40, 174, 319, 498, 643, 817, 850, 937 |
| StyD4I | CCNGG | 4 cut(s) | 240, 334, 859, 873 |
| StyI | CCWWGG | 1 cut(s) | 760 |
| TaaI | ACNGT | 4 cut(s) | 232, 368, 448, 739 |
| TaiI | ACGT | 3 cut(s) | 84, 547, 829 |
| TaqI | TCGA | 5 cut(s) | 116, 219, 356, 730, 789 |
| TasI | AATT | 8 cut(s) | 40, 174, 319, 498, 643, 817, 850, 937 |
| TfiI | GAWTC | 1 cut(s) | 512 |
| Tru1I | TTAA | 5 cut(s) | 246, 318, 402, 666, 912 |
| Tru9I | TTAA | 5 cut(s) | 246, 318, 402, 666, 912 |
| TscAI | CASTG | 1 cut(s) | 613 |
| TseFI | GTSAC | 1 cut(s) | 525 |
| TseI | GCWGC | 1 cut(s) | 919 |
| Tsp45I | GTSAC | 1 cut(s) | 525 |
| TspDTI | ATGAA | 2 cut(s) | 428, 810 |
| TspGWI | ACGGA | 2 cut(s) | 671, 702 |
| TspRI | CASTG | 1 cut(s) | 613 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 750 |
| Vha464I | CTTAAG | 1 cut(s) | 911 |
| VpaK11BI | GGWCC | 2 cut(s) | 677, 864 |
| XapI | RAATTY | 1 cut(s) | 643 |
| XmnI | GAANNNNTTC | 1 cut(s) | 330 |