RLG00000008955

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000533
Physical Location & Seq
Forward (+)
43702860 .. 43716252
13393 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008955

Sequence Viewer

Length: 1548 bp
ATGGAGAAATCGACAATAGCTTCTAGACTATTGCTCTTGAACCTTTTTGTACTTGGATTTCATGCTCAATCAGTAGTTCATTCATTCGCCTATGCATCTGATCATGATTTGAGCTACATGAAGATTGTGAAGGATGCCACTGATCTATCGTTGGAAGATAAATATGACTACATTGTAGTTGGGGGAGGAACAGCAGGGTGTCCATTAGCAGCAACATTATCAGCAAACTACTCGGTGCTTGTTCTTGAAAGGGGTAGTGCTCCTACTGCACATCCAAGTGTCTTACGAGAAGAAAGGTTTCTGAGTAATCTCATGCAAGAAGATGATGGTAAGACCACACCAGCTCAAAAGTTTAAGTCTGAAGATGGTGTTGCTATTGTCAGAGGAAGGGTTCTCGGTGGCTCAAGCATGATCAATGCTGGTTTCTACTCCAAAGCTGACAGGGAATTCTTTGTTAAATATGGAATTAAATGGGACATGGATTTGGTTGATAAGGCATATCACTGGGTTGAAAGCACTATTGTCTCTCGTCCAAATTTGTCACGTTCAAATTGGCAATCTATTGTGAAAGAAGCATTGTTGGAGGCTGGTGTTGGTCCAAACAATGGATTCAGTTTAGATCACATCAAGGGAACTAAAGTTGGCGGTTCTATTTTTGATACATATGGAAGGAGACAAGGTGCTGTTGAATTGCTTAACAGAGGAGACCTAAAGAACTTACGAGTGGGAATTCATGCCACAGTTGAGAAAATCATCTTCTCTTCCAAAGAATCAGGTGTTTCGGCTCGAGGGGTGGTATATATTGATTCCAATGGGAGGTCTCATCGGGCATTTGTACATGGTAAGGGAGAGGTTATTCTGAGTGCAGGGGCAATTGGGAGTGCTCAGCTTCTACTTCTTAGCGGTGTTGGCCCTCAGTCCTATTTATCATCTTTGAAAATCCCAGTTGTTCACCCCCAACCTCACACTAGAAACTTTATGTATGACAATCCTCGTAATTTCATTAACATTTTGGCCTCGTTTCCACTACAGCCCTCAACTGTAAAGATTGTTGGCATTACCGACCATTTCTACATAGAGGCCCTCTCTAGCACACCCATTTCTACTACACCCTACAGTGTTTTTCCAGATCCGGCTAAACTTATAAAGATAAATTCAAGTTTAGCACTCATTGCCATCAAATTGGTAGGACCTTTGTCTTATGGTACTCTGAAGCTTCAATCATCATCTGATGTGACAGTTGGTCCAAATGTCTGCTTCAACTACTTTGCACATCCCTTGGACCTTGCTCGTTGTGTTTCTAGCATGAAGAAGATTGGGGAGTTATTAAATACCAAGTCACTGAAAGTATTTAAGGTTCAAGCTTCGTCGATATCAACAATTTGGCATTTTCACGGTGGATGCCTCGTCGGAAAGGTGGTCGATAGCAGCTTGCGAGTCATGGGAATCGATGCCTTGCGTGTTGTTGACGCATCCACATTCAATTTCACACCAGGGACAAATCCTCAGGCCACGATTATGATGTTGGGCAGGTACTATCTAGTTTAA

Protein Analysis

516

Amino Acids

55.98

Weight (kDa)

9.2

Isoelectric Point (pI)

27.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_N PF00732 55 - 307 2.6e-20 GMC oxidoreductase
GMC_oxred_C PF05199 398 - 510 5.4e-21 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1145
Acc36I ACCTGC 1 cut(s) 1521
AccB7I CCANNNNNTGG 1 cut(s) 605
AciI CCGC 2 cut(s) 645, 903
AclWI GGATC 1 cut(s) 1124
AcsI RAATTY 4 cut(s) 446, 535, 729, 1153
AcuI CTGAAG 2 cut(s) 381, 1232
AfaI GTAC 4 cut(s) 51, 837, 1207, 1535
AfiI CCNNNNNNNGG 2 cut(s) 605, 816
AhdI GACNNNNNGTC 1 cut(s) 1242
AjnI CCWGG 1 cut(s) 1492
AluBI AGCT 8 cut(s) 20, 114, 344, 437, 889, 1216, 1364, 1431
AluI AGCT 8 cut(s) 20, 114, 344, 437, 889, 1216, 1364, 1431
Alw21I GWGCWC 2 cut(s) 262, 886
Alw26I GTCTC 4 cut(s) 529, 667, 699, 825
AlwI GGATC 1 cut(s) 1124
Ama87I CYCGRG 1 cut(s) 786
AoxI GGCC 4 cut(s) 910, 1014, 1080, 1509
ApeKI GCWGC 2 cut(s) 209, 1428
ApoI RAATTY 4 cut(s) 446, 535, 729, 1153
Asp700I GAANNNNTTC 1 cut(s) 297
AspS9I GGNCC 6 cut(s) 596, 911, 1081, 1190, 1244, 1282
AsuHPI GGTGA 1 cut(s) 944
AvaI CYCGRG 1 cut(s) 786
AvaII GGWCC 4 cut(s) 596, 1190, 1244, 1282
AxyI CCTNAGG 1 cut(s) 1506
Bbv12I GWGCWC 2 cut(s) 262, 886
BbvI GCAGC 2 cut(s) 221, 1440
BccI CCATC 3 cut(s) 320, 359, 1184
BcgI CGANNNNNNTGC 2 cut(s) 213, 247
BciT130I CCWGG 1 cut(s) 1494
BclI TGATCA 2 cut(s) 100, 411
BcoDI GTCTC 4 cut(s) 529, 667, 699, 825
BfaI CTAG 5 cut(s) 24, 969, 1089, 1302, 1541
BfmI CTRYAG 2 cut(s) 1028, 1114
BfuAI ACCTGC 1 cut(s) 1521
BisI GCNGC 2 cut(s) 210, 1429
BlpI GCTNAGC 1 cut(s) 885
BlsI GCNGC 2 cut(s) 211, 1430
Bme1390I CCNGG 1 cut(s) 1494
Bme18I GGWCC 4 cut(s) 596, 1190, 1244, 1282
BmeRI GACNNNNNGTC 1 cut(s) 1242
BmeT110I CYCGRG 1 cut(s) 786
BmgT120I GGNCC 6 cut(s) 596, 911, 1081, 1190, 1244, 1282
BmrFI CCNGG 1 cut(s) 1494
BmrI ACTGGG 2 cut(s) 514, 938
BmsI GCATC 5 cut(s) 104, 124, 1391, 1441, 1481
BmuI ACTGGG 2 cut(s) 514, 938
BoxI GACNNNNGTC 1 cut(s) 1195
BplI GAGNNNNNCTC 2 cut(s) 1070, 1102
Bpu1102I GCTNAGC 1 cut(s) 885
BpuEI CTTGAG 1 cut(s) 388
Bsa29I ATCGAT 1 cut(s) 1449
BsaI GGTCTC 2 cut(s) 699, 825
BsaJI CCNNGG 2 cut(s) 1278, 1493
BsaXI ACNNNNNCTCC 2 cut(s) 664, 694
Bsc4I CCNNNNNNNGG 2 cut(s) 605, 816
Bse1I ACTGG 2 cut(s) 509, 944
Bse21I CCTNAGG 1 cut(s) 1506
Bse3DI GCAATG 1 cut(s) 1170
BseBI CCWGG 1 cut(s) 1494
BseCI ATCGAT 1 cut(s) 1449
BseDI CCNNGG 2 cut(s) 1278, 1493
BseGI GGATG 5 cut(s) 139, 271, 1273, 1406, 1472
BseLI CCNNNNNNNGG 2 cut(s) 605, 816
BseMI GCAATG 1 cut(s) 1170
BseMII CTCAG 5 cut(s) 293, 851, 899, 929, 1520
BseNI ACTGG 2 cut(s) 509, 944
BseRI GAGGAG 1 cut(s) 717
BseXI GCAGC 2 cut(s) 221, 1440
BsgI GTGCAG 2 cut(s) 252, 885
BshFI GGCC 4 cut(s) 912, 1016, 1082, 1511
BshVI ATCGAT 1 cut(s) 1449
BsiHKAI GWGCWC 2 cut(s) 262, 886
BsiHKCI CYCGRG 1 cut(s) 786
BsiSI CCGG 1 cut(s) 1133
BslFI GGGAC 2 cut(s) 488, 1510
BslI CCNNNNNNNGG 2 cut(s) 605, 816
BsmAI GTCTC 4 cut(s) 529, 667, 699, 825
BsmFI GGGAC 2 cut(s) 488, 1510
BsnI GGCC 4 cut(s) 912, 1016, 1082, 1511
Bso31I GGTCTC 2 cut(s) 699, 825
BsoBI CYCGRG 1 cut(s) 786
Bsp1286I GDGCHC 2 cut(s) 262, 886
Bsp1407I TGTACA 1 cut(s) 835
Bsp143I GATC 5 cut(s) 100, 142, 411, 619, 1129
Bsp1720I GCTNAGC 1 cut(s) 885
BspACI CCGC 2 cut(s) 645, 903
BspANI GGCC 4 cut(s) 912, 1016, 1082, 1511
BspCNI CTCAG 5 cut(s) 294, 852, 898, 928, 1519
BspDI ATCGAT 1 cut(s) 1449
BspHI TCATGA 1 cut(s) 103
BspMI ACCTGC 1 cut(s) 1521
BspPI GGATC 1 cut(s) 1124
BspTNI GGTCTC 2 cut(s) 699, 825
BsrDI GCAATG 1 cut(s) 1170
BsrGI TGTACA 1 cut(s) 835
BsrI ACTGG 2 cut(s) 509, 944
BssECI CCNNGG 2 cut(s) 1278, 1493
BssMI GATC 5 cut(s) 100, 142, 411, 619, 1129
BssT1I CCWWGG 1 cut(s) 1278
Bst2UI CCWGG 1 cut(s) 1494
Bst4CI ACNGT 5 cut(s) 742, 1042, 1118, 1240, 1397
Bst6I CTCTTC 1 cut(s) 766
BstAPI GCANNNNNTGC 1 cut(s) 1172
BstAUI TGTACA 1 cut(s) 835
BstC8I GCNNGC 1 cut(s) 1433
BstDEI CTNAG 6 cut(s) 302, 860, 885, 899, 915, 1506
BstF5I GGATG 5 cut(s) 139, 271, 1273, 1406, 1472
BstKTI GATC 5 cut(s) 103, 145, 414, 622, 1132
BstMAI GTCTC 4 cut(s) 529, 667, 699, 825
BstMBI GATC 5 cut(s) 100, 142, 411, 619, 1129
BstMWI GCNNNNNNNGC 3 cut(s) 266, 909, 1172
BstNI CCWGG 1 cut(s) 1494
BstPAI GACNNNNGTC 1 cut(s) 1195
BstSCI CCNGG 1 cut(s) 1492
BstSFI CTRYAG 2 cut(s) 1028, 1114
BstV1I GCAGC 2 cut(s) 221, 1440
BstX2I RGATCY 1 cut(s) 1129
BstXI CCANNNNNNTGG 1 cut(s) 1183
BstYI RGATCY 1 cut(s) 1129
Bsu15I ATCGAT 1 cut(s) 1449
Bsu36I CCTNAGG 1 cut(s) 1506
BsuRI GGCC 4 cut(s) 912, 1016, 1082, 1511
BsuTUI ATCGAT 1 cut(s) 1449
BtsCI GGATG 5 cut(s) 139, 271, 1273, 1406, 1472
BtsIMutI CAGTG 4 cut(s) 138, 502, 1123, 1340
BveI ACCTGC 1 cut(s) 1521
Cac8I GCNNGC 1 cut(s) 1433
CciI TCATGA 1 cut(s) 103
Cfr13I GGNCC 6 cut(s) 596, 911, 1081, 1190, 1244, 1282
ClaI ATCGAT 1 cut(s) 1449
CseI GACGC 1 cut(s) 1478
Csp6I GTAC 4 cut(s) 50, 836, 1206, 1534
CviQI GTAC 4 cut(s) 50, 836, 1206, 1534
DdeI CTNAG 6 cut(s) 302, 860, 885, 899, 915, 1506
DpnI GATC 5 cut(s) 102, 144, 413, 621, 1131
DpnII GATC 5 cut(s) 100, 142, 411, 619, 1129
DriI GACNNNNNGTC 1 cut(s) 1242
Eam1104I CTCTTC 1 cut(s) 766
Eam1105I GACNNNNNGTC 1 cut(s) 1242
EarI CTCTTC 1 cut(s) 766
Eco130I CCWWGG 1 cut(s) 1278
Eco31I GGTCTC 2 cut(s) 699, 825
Eco32I GATATC 1 cut(s) 1374
Eco47I GGWCC 4 cut(s) 596, 1190, 1244, 1282
Eco57I CTGAAG 2 cut(s) 381, 1232
Eco81I CCTNAGG 1 cut(s) 1506
Eco88I CYCGRG 1 cut(s) 786
EcoO109I RGGNCCY 2 cut(s) 1081, 1190
EcoRI GAATTC 2 cut(s) 446, 729
EcoRII CCWGG 1 cut(s) 1492
EcoRV GATATC 1 cut(s) 1374
EcoT14I CCWWGG 1 cut(s) 1278
EcoT22I ATGCAT 1 cut(s) 97
ErhI CCWWGG 1 cut(s) 1278
FaqI GGGAC 2 cut(s) 488, 1510
FauNDI CATATG 1 cut(s) 664
FbaI TGATCA 2 cut(s) 100, 411
Fnu4HI GCNGC 2 cut(s) 210, 1429
FokI GGATG 5 cut(s) 146, 258, 1260, 1413, 1459
Fsp4HI GCNGC 2 cut(s) 210, 1429
FspBI CTAG 5 cut(s) 24, 969, 1089, 1302, 1541
GluI GCNGC 2 cut(s) 210, 1429
HaeIII GGCC 4 cut(s) 912, 1016, 1082, 1511
HapII CCGG 1 cut(s) 1133
HgaI GACGC 1 cut(s) 1478
HincII GTYRAC 1 cut(s) 1468
HindII GTYRAC 1 cut(s) 1468
HindIII AAGCTT 2 cut(s) 1214, 1362
HinfI GANTC 5 cut(s) 609, 770, 806, 1437, 1446
HpaII CCGG 1 cut(s) 1133
HphI GGTGA 1 cut(s) 944
Hpy166II GTNNAC 2 cut(s) 952, 1468
Hpy188I TCNGA 8 cut(s) 100, 303, 361, 383, 861, 1212, 1231, 1412
Hpy188III TCNNGA 5 cut(s) 24, 37, 104, 245, 1127
Hpy8I GTNNAC 2 cut(s) 952, 1468
Hpy99I CGWCG 2 cut(s) 1372, 1412
HpyAV CCTTC 3 cut(s) 124, 381, 663
HpyCH4III ACNGT 5 cut(s) 742, 1042, 1118, 1240, 1397
HpyCH4IV ACGT 1 cut(s) 544
HpyCH4V TGCA 5 cut(s) 95, 269, 316, 866, 1271
HpyF10VI GCNNNNNNNGC 3 cut(s) 266, 909, 1172
HpyF3I CTNAG 6 cut(s) 302, 860, 885, 899, 915, 1506
HpySE526I ACGT 1 cut(s) 544
Ksp22I TGATCA 2 cut(s) 100, 411
Kzo9I GATC 5 cut(s) 100, 142, 411, 619, 1129
LmnI GCTCC 1 cut(s) 265
Lsp1109I GCAGC 2 cut(s) 221, 1440
LweI GCATC 5 cut(s) 104, 124, 1391, 1441, 1481
MaeI CTAG 5 cut(s) 24, 969, 1089, 1302, 1541
MaeII ACGT 1 cut(s) 544
MaeIII GTNAC 3 cut(s) 540, 1234, 1338
MalI GATC 5 cut(s) 102, 144, 413, 621, 1131
MboI GATC 5 cut(s) 100, 142, 411, 619, 1129
MboII GAAGA 9 cut(s) 133, 167, 302, 332, 374, 748, 753, 1321, 1324
MfeI CAATTG 1 cut(s) 873
MflI RGATCY 1 cut(s) 1129
MhlI GDGCHC 2 cut(s) 262, 886
MlyI GAGTC 1 cut(s) 1446
MmeI TCCRAC 3 cut(s) 132, 561, 1390
Mph1103I ATGCAT 1 cut(s) 97
MroXI GAANNNNTTC 1 cut(s) 297
MseI TTAA 8 cut(s) 354, 456, 468, 696, 1005, 1328, 1353, 1546
MslI CAYNNNNRTG 2 cut(s) 276, 1517
MspI CCGG 1 cut(s) 1133
MspR9I CCNGG 1 cut(s) 1494
MunI CAATTG 1 cut(s) 873
MvaI CCWGG 1 cut(s) 1494
MwoI GCNNNNNNNGC 3 cut(s) 266, 909, 1172
NdeI CATATG 1 cut(s) 664
NdeII GATC 5 cut(s) 100, 142, 411, 619, 1129
NmuCI GTSAC 3 cut(s) 540, 1234, 1338
NsiI ATGCAT 1 cut(s) 97
PaeR7I CTCGAG 1 cut(s) 786
PagI TCATGA 1 cut(s) 103
PdmI GAANNNNTTC 1 cut(s) 297
PfeI GAWTC 4 cut(s) 609, 770, 806, 1446
PflMI CCANNNNNTGG 1 cut(s) 605
PkrI GCNGC 2 cut(s) 211, 1430
PleI GAGTC 1 cut(s) 1445
PpsI GAGTC 1 cut(s) 1445
PpuMI RGGWCCY 1 cut(s) 1190
PshAI GACNNNNGTC 1 cut(s) 1195
PsiI TTATAA 1 cut(s) 1145
Psp5II RGGWCCY 1 cut(s) 1190
Psp6I CCWGG 1 cut(s) 1492
PspGI CCWGG 1 cut(s) 1492
PspPI GGNCC 6 cut(s) 596, 911, 1081, 1190, 1244, 1282
PspPPI RGGWCCY 1 cut(s) 1190
PspXI VCTCGAGB 1 cut(s) 786
PsrI GAACNNNNNNTAC 2 cut(s) 1341, 1373
PsuI RGATCY 1 cut(s) 1129
RsaI GTAC 4 cut(s) 51, 837, 1207, 1535
RsaNI GTAC 4 cut(s) 50, 836, 1206, 1534
RseI CAYNNNNRTG 2 cut(s) 276, 1517
SaqAI TTAA 8 cut(s) 354, 456, 468, 696, 1005, 1328, 1353, 1546
SatI GCNGC 2 cut(s) 210, 1429
Sau3AI GATC 5 cut(s) 100, 142, 411, 619, 1129
Sau96I GGNCC 6 cut(s) 596, 911, 1081, 1190, 1244, 1282
SchI GAGTC 1 cut(s) 1446
ScrFI CCNGG 1 cut(s) 1494
SduI GDGCHC 2 cut(s) 262, 886
SfaNI GCATC 5 cut(s) 104, 124, 1391, 1441, 1481
SfcI CTRYAG 2 cut(s) 1028, 1114
Sfr274I CTCGAG 1 cut(s) 786
SinI GGWCC 4 cut(s) 596, 1190, 1244, 1282
SlaI CTCGAG 1 cut(s) 786
SmiMI CAYNNNNRTG 2 cut(s) 276, 1517
SmlI CTYRAG 2 cut(s) 403, 786
SmoI CTYRAG 2 cut(s) 403, 786
SsiI CCGC 2 cut(s) 645, 903
SspMI CTAG 5 cut(s) 24, 969, 1089, 1302, 1541
StyD4I CCNGG 1 cut(s) 1492
StyI CCWWGG 1 cut(s) 1278
TaaI ACNGT 5 cut(s) 742, 1042, 1118, 1240, 1397
TaiI ACGT 1 cut(s) 547
TaqI TCGA 5 cut(s) 11, 787, 1370, 1422, 1449
TatI WGTACW 2 cut(s) 49, 835
TfiI GAWTC 4 cut(s) 609, 770, 806, 1446
Tru1I TTAA 8 cut(s) 354, 456, 468, 696, 1005, 1328, 1353, 1546
Tru9I TTAA 8 cut(s) 354, 456, 468, 696, 1005, 1328, 1353, 1546
TscAI CASTG 4 cut(s) 145, 509, 1123, 1347
TseFI GTSAC 3 cut(s) 540, 1234, 1338
TseI GCWGC 2 cut(s) 209, 1428
Tsp45I GTSAC 3 cut(s) 540, 1234, 1338
TspDTI ATGAA 7 cut(s) 50, 68, 72, 134, 722, 991, 1322
TspRI CASTG 4 cut(s) 145, 509, 1123, 1347
Van91I CCANNNNNTGG 1 cut(s) 605
VpaK11BI GGWCC 4 cut(s) 596, 1190, 1244, 1282
XapI RAATTY 4 cut(s) 446, 535, 729, 1153
XbaI TCTAGA 1 cut(s) 23
XhoI CTCGAG 1 cut(s) 786
XmnI GAANNNNTTC 1 cut(s) 297
XspI CTAG 5 cut(s) 24, 969, 1089, 1302, 1541
Zsp2I ATGCAT 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.