Prupe.1G093500_v2.0.a1

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000533
Physical Location & Seq
Reverse (-)
7148502 .. 7149951
1450 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G093500.1

Sequence Viewer

Length: 423 bp
ATGGTAAATCAATATCTCCTTAAGGCATTTGTGCGTGGAAAGGGAGAAGTTATATTGAGTGCCGGGACAATTGGAAGCCCTCAACCTCTGCTACTTAGTGGTGTTGGCCCAAAGTCCTACCTTTCATCTATCAAAATCCCAGTTGTTCACCATGAGCCTAACATTGGGCAGTCTATGCGTGATAATCCTCGCTATTACATTACAATTTTGCCCCCATCTCCACTGGTTCCCTCTGGTGGACAGACTGTTAGTATCACAAAGGATTTCTACGTAGAGACTCTCGCCGGCCCGCCATTTTCTTCCACGCCCTTTAGTCTTTTTCCTCATCCATCTGTTCGCATAAAAATAGATTCAACTTTCGGACATATTGTAGGCAAATTTCCAGGACCCTCGTCCTATGGTTCTCTTACATTGCAATCATGA

Protein Analysis

141

Amino Acids

15.01

Weight (kDa)

9.65

Isoelectric Point (pI)

53.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 290
AcsI RAATTY 1 cut(s) 377
AfiI CCNNNNNNNGG 2 cut(s) 164, 236
AflII CTTAAG 1 cut(s) 20
AgsI TTSAA 1 cut(s) 354
AjnI CCWGG 1 cut(s) 382
Alw26I GTCTC 1 cut(s) 269
AoxI GGCC 2 cut(s) 106, 286
ApoI RAATTY 1 cut(s) 377
AspS9I GGNCC 3 cut(s) 107, 287, 386
AsuC2I CCSGG 1 cut(s) 64
AsuHPI GGTGA 1 cut(s) 140
AvaII GGWCC 1 cut(s) 386
BccI CCATC 2 cut(s) 223, 337
BciT130I CCWGG 1 cut(s) 384
BcnI CCSGG 1 cut(s) 64
BcoDI GTCTC 1 cut(s) 269
BfrI CTTAAG 1 cut(s) 20
Bme1390I CCNGG 2 cut(s) 64, 384
Bme18I GGWCC 1 cut(s) 386
BmgT120I GGNCC 3 cut(s) 107, 287, 386
BmiI GGNNCC 2 cut(s) 228, 388
BmrFI CCNGG 2 cut(s) 64, 384
BmrI ACTGGG 1 cut(s) 134
BmuI ACTGGG 1 cut(s) 134
BoxI GACNNNNGTC 1 cut(s) 391
BpuMI CCSGG 1 cut(s) 64
BsaAI YACGTR 1 cut(s) 271
Bsc4I CCNNNNNNNGG 2 cut(s) 164, 236
Bse118I RCCGGY 1 cut(s) 284
Bse1I ACTGG 2 cut(s) 140, 228
Bse3DI GCAATG 1 cut(s) 410
BseBI CCWGG 1 cut(s) 384
BseGI GGATG 1 cut(s) 325
BseLI CCNNNNNNNGG 2 cut(s) 164, 236
BseMI GCAATG 1 cut(s) 410
BseNI ACTGG 2 cut(s) 140, 228
BshFI GGCC 2 cut(s) 108, 288
BsiSI CCGG 2 cut(s) 63, 285
BslFI GGGAC 1 cut(s) 79
BslI CCNNNNNNNGG 2 cut(s) 164, 236
BsmAI GTCTC 1 cut(s) 269
BsmFI GGGAC 1 cut(s) 79
BsnI GGCC 2 cut(s) 108, 288
BspACI CCGC 1 cut(s) 290
BspANI GGCC 2 cut(s) 108, 288
BspHI TCATGA 1 cut(s) 419
BspLI GGNNCC 2 cut(s) 228, 388
BspTI CTTAAG 1 cut(s) 20
BsrDI GCAATG 1 cut(s) 410
BsrFI RCCGGY 1 cut(s) 284
BsrI ACTGG 2 cut(s) 140, 228
BssAI RCCGGY 1 cut(s) 284
Bst2UI CCWGG 1 cut(s) 384
Bst4CI ACNGT 1 cut(s) 247
BstAFI CTTAAG 1 cut(s) 20
BstAPI GCANNNNNTGC 1 cut(s) 175
BstBAI YACGTR 1 cut(s) 271
BstC8I GCNNGC 2 cut(s) 286, 290
BstDEI CTNAG 1 cut(s) 95
BstF5I GGATG 1 cut(s) 325
BstMAI GTCTC 1 cut(s) 269
BstMWI GCNNNNNNNGC 1 cut(s) 175
BstNI CCWGG 1 cut(s) 384
BstPAI GACNNNNGTC 1 cut(s) 391
BstSCI CCNGG 2 cut(s) 62, 382
BstSNI TACGTA 1 cut(s) 271
BsuRI GGCC 2 cut(s) 108, 288
BtsCI GGATG 1 cut(s) 325
BtsIMutI CAGTG 1 cut(s) 221
Cac8I GCNNGC 2 cut(s) 286, 290
CciI TCATGA 1 cut(s) 419
Cfr10I RCCGGY 1 cut(s) 284
Cfr13I GGNCC 3 cut(s) 107, 287, 386
CviAII CATG 2 cut(s) 152, 420
CviJI RGCY 4 cut(s) 78, 108, 157, 288
CviKI_1 RGCY 4 cut(s) 78, 108, 157, 288
DdeI CTNAG 1 cut(s) 95
Eco105I TACGTA 1 cut(s) 271
Eco47I GGWCC 1 cut(s) 386
EcoO109I RGGNCCY 1 cut(s) 386
EcoRII CCWGG 1 cut(s) 382
FaeI CATG 2 cut(s) 155, 423
FaiI YATR 7 cut(s) 53, 153, 176, 341, 366, 399, 421
FaqI GGGAC 1 cut(s) 79
FatI CATG 2 cut(s) 151, 419
FauI CCCGC 1 cut(s) 297
FokI GGATG 1 cut(s) 312
HaeIII GGCC 2 cut(s) 108, 288
HapII CCGG 2 cut(s) 63, 285
Hin1II CATG 2 cut(s) 155, 423
HinfI GANTC 2 cut(s) 277, 350
HpaII CCGG 2 cut(s) 63, 285
HphI GGTGA 1 cut(s) 140
Hpy166II GTNNAC 2 cut(s) 148, 239
Hpy188I TCNGA 1 cut(s) 362
Hpy188III TCNNGA 1 cut(s) 420
Hpy8I GTNNAC 2 cut(s) 148, 239
HpyCH4III ACNGT 1 cut(s) 247
HpyCH4IV ACGT 1 cut(s) 270
HpyCH4V TGCA 1 cut(s) 415
HpyF10VI GCNNNNNNNGC 1 cut(s) 175
HpyF3I CTNAG 1 cut(s) 95
HpySE526I ACGT 1 cut(s) 270
Hsp92II CATG 2 cut(s) 155, 423
KroI GCCGGC 1 cut(s) 284
KroNI GCCGGC 1 cut(s) 286
LpnPI CCDG 7 cut(s) 76, 153, 209, 219, 298, 369, 396
MaeII ACGT 1 cut(s) 270
MboII GAAGA 1 cut(s) 291
MfeI CAATTG 1 cut(s) 69
MluCI AATT 3 cut(s) 69, 204, 377
MlyI GAGTC 1 cut(s) 271
MnlI CCTC 6 cut(s) 90, 96, 198, 241, 333, 400
MroNI GCCGGC 1 cut(s) 284
MseI TTAA 1 cut(s) 21
MspCI CTTAAG 1 cut(s) 20
MspI CCGG 2 cut(s) 63, 285
MspR9I CCNGG 2 cut(s) 64, 384
MunI CAATTG 1 cut(s) 69
MvaI CCWGG 1 cut(s) 384
MwoI GCNNNNNNNGC 1 cut(s) 175
NaeI GCCGGC 1 cut(s) 286
NciI CCSGG 1 cut(s) 64
NgoMIV GCCGGC 1 cut(s) 284
NlaIII CATG 2 cut(s) 155, 423
NlaIV GGNNCC 2 cut(s) 228, 388
PagI TCATGA 1 cut(s) 419
PdiI GCCGGC 1 cut(s) 286
PfeI GAWTC 1 cut(s) 350
PfoI TCCNGGA 1 cut(s) 382
PleI GAGTC 1 cut(s) 271
PpsI GAGTC 1 cut(s) 271
Ppu21I YACGTR 1 cut(s) 271
PpuMI RGGWCCY 1 cut(s) 386
PshAI GACNNNNGTC 1 cut(s) 391
Psp5II RGGWCCY 1 cut(s) 386
Psp6I CCWGG 1 cut(s) 382
PspGI CCWGG 1 cut(s) 382
PspN4I GGNNCC 2 cut(s) 228, 388
PspPI GGNCC 3 cut(s) 107, 287, 386
PspPPI RGGWCCY 1 cut(s) 386
SaqAI TTAA 1 cut(s) 21
Sau96I GGNCC 3 cut(s) 107, 287, 386
SchI GAGTC 1 cut(s) 271
ScrFI CCNGG 2 cut(s) 64, 384
SetI ASST 3 cut(s) 88, 123, 273
SinI GGWCC 1 cut(s) 386
SmlI CTYRAG 1 cut(s) 20
SmoI CTYRAG 1 cut(s) 20
SnaBI TACGTA 1 cut(s) 271
Sse9I AATT 3 cut(s) 69, 204, 377
SsiI CCGC 1 cut(s) 290
StyD4I CCNGG 2 cut(s) 62, 382
TaaI ACNGT 1 cut(s) 247
TaiI ACGT 1 cut(s) 273
TasI AATT 3 cut(s) 69, 204, 377
TfiI GAWTC 1 cut(s) 350
Tru1I TTAA 1 cut(s) 21
Tru9I TTAA 1 cut(s) 21
TscAI CASTG 1 cut(s) 228
TspDTI ATGAA 1 cut(s) 114
TspRI CASTG 1 cut(s) 228
Vha464I CTTAAG 1 cut(s) 20
VpaK11BI GGWCC 1 cut(s) 386
XapI RAATTY 1 cut(s) 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.