Rh4BG113900

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000533
Physical Location & Seq
Reverse (-)
19244475 .. 19261653
17179 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG113900.1

Sequence Viewer

Length: 1086 bp
ATGGAGCTGTGCTCCAATGATGAAGATTGCCTTGTATCTCCATTCCCAATAACTTTGAACCCAATGCCGGTGTTGGAGTGCATGACTACTGCAAGCCACTTGTCGATTCATTCATTCGCCTATGCATTTGCATCTGATCATGATTTGAGCTACATGAAGATTGTGAAGGATGCCACTGATCTATTGTTGGAAGAGAAATATGACTACATTGTAGTTGGGGGAGGAACCGCAGGGTGTCCATCAGCAGCAACATTATCAGCAAACTACTCGGTGCTTCTTCTTGAAAGGGGTGGTGCTCCTACTGCACATCCAAGTGTCTTACGAGAAGAAGGGTTTCTGAGTAATCTCATGCAAGAAGATGATGGTAAGACCACACCAACTCAAAAGTTTAAGTCAGAAGATGGTGTTGCTATTGTCAGAGGAAGGGTTCTCGGTGGCACAAGTATGATCAATGCTGGTTTCTACTCCAAAGCTGACAGCGAATTCTTTGTTAAATCTGAAATTAAATGGGACATGAATTTGGTTGATAAGGCATATCACTGGGTTGAAAACACTGTCGTCTCTCGCCCAAATCTGTCACCTACAAATTGGCAATTTGTTGTGAAAGAAGCATTGTTGGAGGCTGGTGTTGGTCCAAACAATGGATTCAGTTTGGATCACATTAAGGGAACTAAAGTTGGCGGTTCTACTTTTGATACACATGGAAGGAGACACGGAGCAGTTGAATTGCTTAACAGAGGAAACCTAAAGAACTTGCGAGTGGGAATTCATGCCACAGTGGAGAAAATCATCTTCTCTTCCAAAGAATCAGGTGTTTCGGCTCGAGGGGTGGTCTATATTGATTCCAATGGGAGGTCTTATCGGGCATTTGTACGTGGTAAGGGAGAGGTTATTCTGAGTGCAGGGGCAATTGGGAGTCCTCAGCTTCTACTTCTTAGCGGTGTTGGCCCTCAGTCCTATCTATCATCTTTGAAAATCCCAGTTGTTCACCCCCAACCTCACACTGGAAACTTTATGTATGACAATCCTCGTACATTGGCATTGAGATGCTGCAACAAAGATCTTCTTAAGCGATGCAGCGTATAA

Protein Analysis

361

Amino Acids

39.2

Weight (kDa)

6.83

Isoelectric Point (pI)

34.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_N PF00732 67 - 320 2e-20 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 641
AciI CCGC 3 cut(s) 228, 681, 939
AclWI GGATC 1 cut(s) 663
AcsI RAATTY 3 cut(s) 482, 517, 765
AfaI GTAC 2 cut(s) 873, 1033
AfiI CCNNNNNNNGG 4 cut(s) 67, 641, 852, 1004
AflII CTTAAG 1 cut(s) 1067
AgsI TTSAA 5 cut(s) 58, 284, 548, 725, 973
AluBI AGCT 4 cut(s) 7, 150, 473, 925
AluI AGCT 4 cut(s) 7, 150, 473, 925
Alw21I GWGCWC 2 cut(s) 14, 298
Alw26I GTCTC 2 cut(s) 565, 703
AlwI GGATC 1 cut(s) 663
Ama87I CYCGRG 1 cut(s) 822
AoxI GGCC 1 cut(s) 946
ApeKI GCWGC 3 cut(s) 245, 1050, 1077
ApoI RAATTY 3 cut(s) 482, 517, 765
Asp700I GAANNNNTTC 1 cut(s) 333
AspS9I GGNCC 2 cut(s) 632, 947
AsuHPI GGTGA 2 cut(s) 570, 980
AvaI CYCGRG 1 cut(s) 822
AvaII GGWCC 1 cut(s) 632
Bbv12I GWGCWC 2 cut(s) 14, 298
BbvCI CCTCAGC 1 cut(s) 921
BbvI GCAGC 2 cut(s) 257, 1037
BccI CCATC 3 cut(s) 247, 356, 395
BcgI CGANNNNNNTGC 2 cut(s) 249, 283
BclI TGATCA 2 cut(s) 136, 447
BcoDI GTCTC 2 cut(s) 565, 703
BfrI CTTAAG 1 cut(s) 1067
BglII AGATCT 1 cut(s) 1060
BisI GCNGC 3 cut(s) 246, 1051, 1078
BlsI GCNGC 3 cut(s) 247, 1052, 1079
Bme18I GGWCC 1 cut(s) 632
BmeT110I CYCGRG 1 cut(s) 822
BmgT120I GGNCC 2 cut(s) 632, 947
BmiI GGNNCC 1 cut(s) 226
BmrI ACTGGG 2 cut(s) 550, 974
BmsI GCATC 4 cut(s) 140, 160, 1037, 1064
BmuI ACTGGG 2 cut(s) 550, 974
BplI GAGNNNNNCTC 1 cut(s) 28
Bpu10I CCTNAGC 1 cut(s) 921
BsaAI YACGTR 1 cut(s) 875
Bsc4I CCNNNNNNNGG 4 cut(s) 67, 641, 852, 1004
Bse118I RCCGGY 1 cut(s) 67
Bse1I ACTGG 3 cut(s) 545, 980, 1009
BseGI GGATG 2 cut(s) 175, 307
BseLI CCNNNNNNNGG 4 cut(s) 67, 641, 852, 1004
BseMII CTCAG 4 cut(s) 329, 887, 935, 965
BseNI ACTGG 3 cut(s) 545, 980, 1009
BseXI GCAGC 2 cut(s) 257, 1037
BsgI GTGCAG 2 cut(s) 288, 921
BshFI GGCC 1 cut(s) 948
BsiHKAI GWGCWC 2 cut(s) 14, 298
BsiHKCI CYCGRG 1 cut(s) 822
BsiSI CCGG 1 cut(s) 68
BslFI GGGAC 1 cut(s) 524
BslI CCNNNNNNNGG 4 cut(s) 67, 641, 852, 1004
BsmAI GTCTC 2 cut(s) 565, 703
BsmBI CGTCTC 1 cut(s) 565
BsmFI GGGAC 1 cut(s) 524
BsnI GGCC 1 cut(s) 948
BsoBI CYCGRG 1 cut(s) 822
Bsp1286I GDGCHC 2 cut(s) 14, 298
Bsp143I GATC 5 cut(s) 136, 178, 447, 655, 1060
BspACI CCGC 3 cut(s) 228, 681, 939
BspANI GGCC 1 cut(s) 948
BspCNI CTCAG 4 cut(s) 330, 888, 934, 964
BspHI TCATGA 1 cut(s) 139
BspLI GGNNCC 1 cut(s) 226
BspPI GGATC 1 cut(s) 663
BspTI CTTAAG 1 cut(s) 1067
BsrFI RCCGGY 1 cut(s) 67
BsrI ACTGG 3 cut(s) 545, 980, 1009
BssAI RCCGGY 1 cut(s) 67
BssMI GATC 5 cut(s) 136, 178, 447, 655, 1060
Bst4CI ACNGT 2 cut(s) 556, 778
Bst6I CTCTTC 2 cut(s) 186, 802
BstAFI CTTAAG 1 cut(s) 1067
BstBAI YACGTR 1 cut(s) 875
BstC8I GCNNGC 1 cut(s) 94
BstDEI CTNAG 5 cut(s) 338, 896, 921, 935, 951
BstF5I GGATG 2 cut(s) 175, 307
BstKTI GATC 5 cut(s) 139, 181, 450, 658, 1063
BstMAI GTCTC 2 cut(s) 565, 703
BstMBI GATC 5 cut(s) 136, 178, 447, 655, 1060
BstMWI GCNNNNNNNGC 2 cut(s) 302, 945
BstV1I GCAGC 2 cut(s) 257, 1037
BstX2I RGATCY 1 cut(s) 1060
BstYI RGATCY 1 cut(s) 1060
BsuRI GGCC 1 cut(s) 948
BtsCI GGATG 2 cut(s) 175, 307
BtsIMutI CAGTG 5 cut(s) 174, 538, 552, 783, 1002
Cac8I GCNNGC 1 cut(s) 94
CciI TCATGA 1 cut(s) 139
Cfr10I RCCGGY 1 cut(s) 67
Cfr13I GGNCC 2 cut(s) 632, 947
Csp6I GTAC 2 cut(s) 872, 1032
CviAII CATG 7 cut(s) 82, 140, 154, 349, 514, 701, 770
CviJI RGCY 8 cut(s) 7, 96, 150, 473, 623, 821, 925, 948
CviKI_1 RGCY 8 cut(s) 7, 96, 150, 473, 623, 821, 925, 948
CviQI GTAC 2 cut(s) 872, 1032
DdeI CTNAG 5 cut(s) 338, 896, 921, 935, 951
DpnI GATC 5 cut(s) 138, 180, 449, 657, 1062
DpnII GATC 5 cut(s) 136, 178, 447, 655, 1060
Eam1104I CTCTTC 2 cut(s) 186, 802
EarI CTCTTC 2 cut(s) 186, 802
Eco47I GGWCC 1 cut(s) 632
Eco88I CYCGRG 1 cut(s) 822
EcoRI GAATTC 2 cut(s) 482, 765
EcoT22I ATGCAT 1 cut(s) 127
Esp3I CGTCTC 1 cut(s) 565
FaeI CATG 7 cut(s) 85, 143, 157, 352, 517, 704, 773
FalI AAGNNNNNCTT 4 cut(s) 15, 47, 1050, 1082
FaqI GGGAC 1 cut(s) 524
FatI CATG 7 cut(s) 81, 139, 153, 348, 513, 700, 769
FbaI TGATCA 2 cut(s) 136, 447
Fnu4HI GCNGC 3 cut(s) 246, 1051, 1078
FokI GGATG 2 cut(s) 182, 294
Fsp4HI GCNGC 3 cut(s) 246, 1051, 1078
GluI GCNGC 3 cut(s) 246, 1051, 1078
HaeIII GGCC 1 cut(s) 948
HapII CCGG 1 cut(s) 68
Hin1II CATG 7 cut(s) 85, 143, 157, 352, 517, 704, 773
HinfI GANTC 5 cut(s) 106, 645, 806, 842, 916
HpaII CCGG 1 cut(s) 68
HphI GGTGA 2 cut(s) 570, 980
Hpy166II GTNNAC 1 cut(s) 988
Hpy188I TCNGA 6 cut(s) 136, 339, 397, 419, 499, 897
Hpy188III TCNNGA 2 cut(s) 140, 281
Hpy8I GTNNAC 1 cut(s) 988
HpyAV CCTTC 4 cut(s) 160, 323, 417, 699
HpyCH4III ACNGT 2 cut(s) 556, 778
HpyCH4IV ACGT 1 cut(s) 874
HpyCH4V TGCA 9 cut(s) 81, 92, 125, 131, 305, 352, 902, 1053, 1077
HpyF10VI GCNNNNNNNGC 2 cut(s) 302, 945
HpyF3I CTNAG 5 cut(s) 338, 896, 921, 935, 951
HpySE526I ACGT 1 cut(s) 874
Hsp92II CATG 7 cut(s) 85, 143, 157, 352, 517, 704, 773
Ksp22I TGATCA 2 cut(s) 136, 447
Kzo9I GATC 5 cut(s) 136, 178, 447, 655, 1060
LmnI GCTCC 4 cut(s) 4, 17, 301, 716
LpnPI CCDG 9 cut(s) 81, 216, 441, 526, 609, 795, 888, 990, 993
Lsp1109I GCAGC 2 cut(s) 257, 1037
LweI GCATC 4 cut(s) 140, 160, 1037, 1064
MaeII ACGT 1 cut(s) 874
MaeIII GTNAC 1 cut(s) 576
MalI GATC 5 cut(s) 138, 180, 449, 657, 1062
MboI GATC 5 cut(s) 136, 178, 447, 655, 1060
MfeI CAATTG 1 cut(s) 909
MflI RGATCY 1 cut(s) 1060
MhlI GDGCHC 2 cut(s) 14, 298
MluCI AATT 8 cut(s) 482, 501, 517, 586, 593, 725, 765, 909
MlyI GAGTC 1 cut(s) 925
MmeI TCCRAC 3 cut(s) 54, 168, 597
Mph1103I ATGCAT 1 cut(s) 127
MroXI GAANNNNTTC 1 cut(s) 333
MseI TTAA 6 cut(s) 390, 492, 504, 663, 732, 1068
MslI CAYNNNNRTG 3 cut(s) 312, 443, 1045
MspCI CTTAAG 1 cut(s) 1067
MspI CCGG 1 cut(s) 68
MunI CAATTG 1 cut(s) 909
MwoI GCNNNNNNNGC 2 cut(s) 302, 945
NdeII GATC 5 cut(s) 136, 178, 447, 655, 1060
NlaIII CATG 7 cut(s) 85, 143, 157, 352, 517, 704, 773
NlaIV GGNNCC 1 cut(s) 226
NmuCI GTSAC 1 cut(s) 576
NsiI ATGCAT 1 cut(s) 127
PaeR7I CTCGAG 1 cut(s) 822
PagI TCATGA 1 cut(s) 139
PdmI GAANNNNTTC 1 cut(s) 333
PfeI GAWTC 4 cut(s) 106, 645, 806, 842
PflMI CCANNNNNTGG 1 cut(s) 641
PkrI GCNGC 3 cut(s) 247, 1052, 1079
PleI GAGTC 1 cut(s) 924
PpsI GAGTC 1 cut(s) 924
Ppu21I YACGTR 1 cut(s) 875
PspN4I GGNNCC 1 cut(s) 226
PspPI GGNCC 2 cut(s) 632, 947
PspXI VCTCGAGB 1 cut(s) 822
PsuI RGATCY 1 cut(s) 1060
RsaI GTAC 2 cut(s) 873, 1033
RsaNI GTAC 2 cut(s) 872, 1032
RseI CAYNNNNRTG 3 cut(s) 312, 443, 1045
SaqAI TTAA 6 cut(s) 390, 492, 504, 663, 732, 1068
SatI GCNGC 3 cut(s) 246, 1051, 1078
Sau3AI GATC 5 cut(s) 136, 178, 447, 655, 1060
Sau96I GGNCC 2 cut(s) 632, 947
SchI GAGTC 1 cut(s) 925
SduI GDGCHC 2 cut(s) 14, 298
SfaNI GCATC 4 cut(s) 140, 160, 1037, 1064
Sfr274I CTCGAG 1 cut(s) 822
SinI GGWCC 1 cut(s) 632
SlaI CTCGAG 1 cut(s) 822
SmiMI CAYNNNNRTG 3 cut(s) 312, 443, 1045
SmlI CTYRAG 2 cut(s) 822, 1067
SmoI CTYRAG 2 cut(s) 822, 1067
Sse9I AATT 8 cut(s) 482, 501, 517, 586, 593, 725, 765, 909
SsiI CCGC 3 cut(s) 228, 681, 939
TaaI ACNGT 2 cut(s) 556, 778
TaiI ACGT 1 cut(s) 877
TaqI TCGA 2 cut(s) 104, 823
TasI AATT 8 cut(s) 482, 501, 517, 586, 593, 725, 765, 909
TfiI GAWTC 4 cut(s) 106, 645, 806, 842
Tru1I TTAA 6 cut(s) 390, 492, 504, 663, 732, 1068
Tru9I TTAA 6 cut(s) 390, 492, 504, 663, 732, 1068
TscAI CASTG 5 cut(s) 181, 545, 559, 783, 1009
TseFI GTSAC 1 cut(s) 576
TseI GCWGC 3 cut(s) 245, 1050, 1077
Tsp45I GTSAC 1 cut(s) 576
TspDTI ATGAA 6 cut(s) 36, 98, 102, 170, 530, 758
TspGWI ACGGA 1 cut(s) 729
TspRI CASTG 5 cut(s) 181, 545, 559, 783, 1009
Van91I CCANNNNNTGG 1 cut(s) 641
Vha464I CTTAAG 1 cut(s) 1067
VpaK11BI GGWCC 1 cut(s) 632
XapI RAATTY 3 cut(s) 482, 517, 765
XcmI CCANNNNNNNNNTGG 1 cut(s) 1001
XhoI CTCGAG 1 cut(s) 822
XmnI GAANNNNTTC 1 cut(s) 333
Zsp2I ATGCAT 1 cut(s) 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.