(R)-mandelonitrile lyase
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 1593 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 590, 1455 |
| AciI | CCGC | 5 cut(s) | 188, 1240, 1262, 1385, 1413 |
| AclWI | GGATC | 6 cut(s) | 625, 1109, 1275, 1526, 1539, 1552 |
| AcoI | YGGCCR | 1 cut(s) | 363 |
| AcsI | RAATTY | 6 cut(s) | 116, 437, 1138, 1167, 1321, 1556 |
| AcyI | GRCGYC | 1 cut(s) | 329 |
| AfaI | GTAC | 6 cut(s) | 41, 158, 184, 224, 634, 1163 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 495, 590, 953, 1455 |
| AflIII | ACRYGT | 1 cut(s) | 1531 |
| AgsI | TTSAA | 9 cut(s) | 74, 461, 503, 1010, 1143, 1246, 1330, 1372, 1555 |
| AjnI | CCWGG | 4 cut(s) | 613, 1171, 1354, 1564 |
| AluBI | AGCT | 3 cut(s) | 108, 335, 668 |
| AluI | AGCT | 3 cut(s) | 108, 335, 668 |
| Alw21I | GWGCWC | 2 cut(s) | 234, 1030 |
| Alw26I | GTCTC | 3 cut(s) | 652, 1058, 1433 |
| Alw44I | GTGCAC | 1 cut(s) | 1026 |
| AlwI | GGATC | 6 cut(s) | 625, 1109, 1275, 1526, 1539, 1552 |
| Ama87I | CYCGRG | 2 cut(s) | 941, 1072 |
| AoxI | GGCC | 4 cut(s) | 363, 486, 813, 895 |
| ApaLI | GTGCAC | 1 cut(s) | 1026 |
| ApeKI | GCWGC | 3 cut(s) | 192, 203, 1201 |
| ApoI | RAATTY | 6 cut(s) | 116, 437, 1138, 1167, 1321, 1556 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 915, 947 |
| AspLEI | GCGC | 1 cut(s) | 851 |
| AspS9I | GGNCC | 5 cut(s) | 896, 1175, 1229, 1382, 1569 |
| AsuC2I | CCSGG | 1 cut(s) | 853 |
| AsuHPI | GGTGA | 3 cut(s) | 929, 1316, 1554 |
| AvaI | CYCGRG | 2 cut(s) | 941, 1072 |
| AvaII | GGWCC | 4 cut(s) | 1175, 1229, 1382, 1569 |
| BaeGI | GKGCMC | 1 cut(s) | 1030 |
| BalI | TGGCCA | 1 cut(s) | 365 |
| BamHI | GGATCC | 1 cut(s) | 1544 |
| BbsI | GAAGAC | 2 cut(s) | 43, 321 |
| Bbv12I | GWGCWC | 2 cut(s) | 234, 1030 |
| BbvI | GCAGC | 3 cut(s) | 179, 215, 1188 |
| BccI | CCATC | 7 cut(s) | 97, 314, 350, 1126, 1358, 1490, 1535 |
| BceAI | ACGGC | 1 cut(s) | 127 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 207, 241 |
| BciT130I | CCWGG | 4 cut(s) | 615, 1173, 1356, 1566 |
| BclI | TGATCA | 2 cut(s) | 94, 402 |
| BcnI | CCSGG | 1 cut(s) | 853 |
| BcoDI | GTCTC | 3 cut(s) | 652, 1058, 1433 |
| BfaI | CTAG | 6 cut(s) | 239, 311, 423, 686, 771, 1023 |
| BfmI | CTRYAG | 1 cut(s) | 1202 |
| BfoI | RGCGCY | 1 cut(s) | 852 |
| BfuAI | ACCTGC | 1 cut(s) | 1593 |
| BisI | GCNGC | 3 cut(s) | 193, 204, 1202 |
| BlsI | GCNGC | 3 cut(s) | 194, 205, 1203 |
| BmcAI | AGTACT | 1 cut(s) | 224 |
| Bme1390I | CCNGG | 5 cut(s) | 615, 853, 1173, 1356, 1566 |
| Bme18I | GGWCC | 4 cut(s) | 1175, 1229, 1382, 1569 |
| BmeT110I | CYCGRG | 2 cut(s) | 941, 1072 |
| BmgT120I | GGNCC | 5 cut(s) | 896, 1175, 1229, 1382, 1569 |
| BmiI | GGNNCC | 3 cut(s) | 1177, 1546, 1570 |
| BmrFI | CCNGG | 5 cut(s) | 615, 853, 1173, 1356, 1566 |
| BmrI | ACTGGG | 1 cut(s) | 923 |
| BmsI | GCATC | 4 cut(s) | 1006, 1424, 1463, 1611 |
| BmuI | ACTGGG | 1 cut(s) | 923 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1180 |
| BpiI | GAAGAC | 2 cut(s) | 43, 321 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1055, 1087 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1196 |
| BpuEI | CTTGAG | 2 cut(s) | 1329, 1563 |
| BpuMI | CCSGG | 1 cut(s) | 853 |
| BsaAI | YACGTR | 2 cut(s) | 824, 1532 |
| BsaHI | GRCGYC | 1 cut(s) | 329 |
| BsaJI | CCNNGG | 4 cut(s) | 852, 1419, 1465, 1565 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 495, 590, 953, 1455 |
| Bse1I | ACTGG | 2 cut(s) | 929, 1017 |
| BseBI | CCWGG | 4 cut(s) | 615, 1173, 1356, 1566 |
| BseDI | CCNNGG | 4 cut(s) | 852, 1419, 1465, 1565 |
| BseGI | GGATG | 4 cut(s) | 347, 1021, 1415, 1478 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 495, 590, 953, 1455 |
| BseMII | CTCAG | 2 cut(s) | 57, 465 |
| BseNI | ACTGG | 2 cut(s) | 929, 1017 |
| BseSI | GKGCMC | 1 cut(s) | 1030 |
| BseXI | GCAGC | 3 cut(s) | 179, 215, 1188 |
| BshFI | GGCC | 4 cut(s) | 365, 488, 815, 897 |
| BsiHKAI | GWGCWC | 2 cut(s) | 234, 1030 |
| BsiHKCI | CYCGRG | 2 cut(s) | 941, 1072 |
| BsiSI | CCGG | 1 cut(s) | 852 |
| BslFI | GGGAC | 2 cut(s) | 479, 1582 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 495, 590, 953, 1455 |
| BsmAI | GTCTC | 3 cut(s) | 652, 1058, 1433 |
| BsmFI | GGGAC | 2 cut(s) | 479, 1582 |
| BsnI | GGCC | 4 cut(s) | 365, 488, 815, 897 |
| BsoBI | CYCGRG | 2 cut(s) | 941, 1072 |
| Bsp1286I | GDGCHC | 2 cut(s) | 234, 1030 |
| Bsp1407I | TGTACA | 1 cut(s) | 1161 |
| Bsp143I | GATC | 7 cut(s) | 94, 402, 617, 1114, 1267, 1518, 1544 |
| Bsp19I | CCATGG | 1 cut(s) | 1465 |
| BspACI | CCGC | 5 cut(s) | 188, 1240, 1262, 1385, 1413 |
| BspANI | GGCC | 4 cut(s) | 365, 488, 815, 897 |
| BspCNI | CTCAG | 2 cut(s) | 58, 464 |
| BspHI | TCATGA | 2 cut(s) | 97, 1590 |
| BspLI | GGNNCC | 3 cut(s) | 1177, 1546, 1570 |
| BspMAI | CTGCAG | 1 cut(s) | 1206 |
| BspMI | ACCTGC | 1 cut(s) | 1593 |
| BspPI | GGATC | 6 cut(s) | 625, 1109, 1275, 1526, 1539, 1552 |
| BsrGI | TGTACA | 1 cut(s) | 1161 |
| BsrI | ACTGG | 2 cut(s) | 929, 1017 |
| BssECI | CCNNGG | 4 cut(s) | 852, 1419, 1465, 1565 |
| BssMI | GATC | 7 cut(s) | 94, 402, 617, 1114, 1267, 1518, 1544 |
| BssNI | GRCGYC | 1 cut(s) | 329 |
| BssT1I | CCWWGG | 1 cut(s) | 1465 |
| Bst2UI | CCWGG | 4 cut(s) | 615, 1173, 1356, 1566 |
| Bst4CI | ACNGT | 2 cut(s) | 727, 1444 |
| Bst6I | CTCTTC | 1 cut(s) | 751 |
| BstACI | GRCGYC | 1 cut(s) | 329 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 719 |
| BstAUI | TGTACA | 1 cut(s) | 1161 |
| BstBAI | YACGTR | 2 cut(s) | 824, 1532 |
| BstC8I | GCNNGC | 2 cut(s) | 190, 333 |
| BstDEI | CTNAG | 5 cut(s) | 66, 451, 621, 884, 1196 |
| BstDSI | CCRYGG | 2 cut(s) | 1419, 1465 |
| BstEII | GGTNACC | 1 cut(s) | 1077 |
| BstF5I | GGATG | 4 cut(s) | 347, 1021, 1415, 1478 |
| BstH2I | RGCGCY | 1 cut(s) | 852 |
| BstHHI | GCGC | 1 cut(s) | 851 |
| BstKTI | GATC | 7 cut(s) | 97, 405, 620, 1117, 1270, 1521, 1547 |
| BstMAI | GTCTC | 3 cut(s) | 652, 1058, 1433 |
| BstMBI | GATC | 7 cut(s) | 94, 402, 617, 1114, 1267, 1518, 1544 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 719, 1025, 1201 |
| BstNI | CCWGG | 4 cut(s) | 615, 1173, 1356, 1566 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1180 |
| BstPI | GGTNACC | 1 cut(s) | 1077 |
| BstSCI | CCNGG | 5 cut(s) | 613, 851, 1171, 1354, 1564 |
| BstSFI | CTRYAG | 1 cut(s) | 1202 |
| BstSLI | GKGCMC | 1 cut(s) | 1030 |
| BstV1I | GCAGC | 3 cut(s) | 179, 215, 1188 |
| BstV2I | GAAGAC | 2 cut(s) | 43, 321 |
| BstX2I | RGATCY | 3 cut(s) | 617, 1267, 1544 |
| BstYI | RGATCY | 3 cut(s) | 617, 1267, 1544 |
| BsuRI | GGCC | 4 cut(s) | 365, 488, 815, 897 |
| BtgI | CCRYGG | 2 cut(s) | 1419, 1465 |
| BtsCI | GGATG | 4 cut(s) | 347, 1021, 1415, 1478 |
| BtsI | GCAGTG | 1 cut(s) | 256 |
| BtsIMutI | CAGTG | 3 cut(s) | 132, 256, 732 |
| BveI | ACCTGC | 1 cut(s) | 1593 |
| Cac8I | GCNNGC | 2 cut(s) | 190, 333 |
| CciI | TCATGA | 2 cut(s) | 97, 1590 |
| CfoI | GCGC | 1 cut(s) | 851 |
| Cfr13I | GGNCC | 5 cut(s) | 896, 1175, 1229, 1382, 1569 |
| CpoI | CGGWCCG | 1 cut(s) | 1382 |
| CseI | GACGC | 2 cut(s) | 269, 337 |
| Csp6I | GTAC | 6 cut(s) | 40, 157, 183, 223, 633, 1162 |
| CspI | CGGWCCG | 1 cut(s) | 1382 |
| CviAII | CATG | 9 cut(s) | 98, 112, 307, 400, 469, 662, 719, 1466, 1591 |
| CviQI | GTAC | 6 cut(s) | 40, 157, 183, 223, 633, 1162 |
| DdeI | CTNAG | 5 cut(s) | 66, 451, 621, 884, 1196 |
| DpnI | GATC | 7 cut(s) | 96, 404, 619, 1116, 1269, 1520, 1546 |
| DpnII | GATC | 7 cut(s) | 94, 402, 617, 1114, 1267, 1518, 1544 |
| DraI | TTTAAA | 1 cut(s) | 603 |
| EaeI | YGGCCR | 1 cut(s) | 363 |
| Eam1104I | CTCTTC | 1 cut(s) | 751 |
| EarI | CTCTTC | 1 cut(s) | 751 |
| Eco130I | CCWWGG | 1 cut(s) | 1465 |
| Eco147I | AGGCCT | 2 cut(s) | 488, 815 |
| Eco47I | GGWCC | 4 cut(s) | 1175, 1229, 1382, 1569 |
| Eco88I | CYCGRG | 2 cut(s) | 941, 1072 |
| Eco91I | GGTNACC | 1 cut(s) | 1077 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1175 |
| EcoO65I | GGTNACC | 1 cut(s) | 1077 |
| EcoRII | CCWGG | 4 cut(s) | 613, 1171, 1354, 1564 |
| EcoT14I | CCWWGG | 1 cut(s) | 1465 |
| ErhI | CCWWGG | 1 cut(s) | 1465 |
| FaeI | CATG | 9 cut(s) | 101, 115, 310, 403, 472, 665, 722, 1469, 1594 |
| FaqI | GGGAC | 2 cut(s) | 479, 1582 |
| FatI | CATG | 9 cut(s) | 97, 111, 306, 399, 468, 661, 718, 1465, 1590 |
| FauI | CCCGC | 1 cut(s) | 181 |
| FbaI | TGATCA | 2 cut(s) | 94, 402 |
| Fnu4HI | GCNGC | 3 cut(s) | 193, 204, 1202 |
| FokI | GGATG | 4 cut(s) | 334, 1028, 1402, 1485 |
| Fsp4HI | GCNGC | 3 cut(s) | 193, 204, 1202 |
| FspBI | CTAG | 6 cut(s) | 239, 311, 423, 686, 771, 1023 |
| GlaI | GCGC | 1 cut(s) | 850 |
| GluI | GCNGC | 3 cut(s) | 193, 204, 1202 |
| HaeII | RGCGCY | 1 cut(s) | 852 |
| HaeIII | GGCC | 4 cut(s) | 365, 488, 815, 897 |
| HapII | CCGG | 1 cut(s) | 852 |
| HgaI | GACGC | 2 cut(s) | 269, 337 |
| HhaI | GCGC | 1 cut(s) | 851 |
| Hin1I | GRCGYC | 1 cut(s) | 329 |
| Hin1II | CATG | 9 cut(s) | 101, 115, 310, 403, 472, 665, 722, 1469, 1594 |
| Hin6I | GCGC | 1 cut(s) | 849 |
| HinP1I | GCGC | 1 cut(s) | 849 |
| HinfI | GANTC | 6 cut(s) | 292, 735, 777, 791, 902, 1509 |
| HpaII | CCGG | 1 cut(s) | 852 |
| HphI | GGTGA | 3 cut(s) | 929, 1316, 1554 |
| Hpy166II | GTNNAC | 5 cut(s) | 157, 345, 937, 1028, 1162 |
| Hpy188I | TCNGA | 5 cut(s) | 67, 94, 352, 1151, 1382 |
| Hpy8I | GTNNAC | 5 cut(s) | 157, 345, 937, 1028, 1162 |
| HpyAV | CCTTC | 4 cut(s) | 547, 551, 648, 826 |
| HpyCH4III | ACNGT | 2 cut(s) | 727, 1444 |
| HpyCH4IV | ACGT | 3 cut(s) | 535, 823, 1531 |
| HpyCH4V | TGCA | 6 cut(s) | 26, 263, 713, 1028, 1204, 1256 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 719, 1025, 1201 |
| HpyF3I | CTNAG | 5 cut(s) | 66, 451, 621, 884, 1196 |
| HpySE526I | ACGT | 3 cut(s) | 535, 823, 1531 |
| Hsp92I | GRCGYC | 1 cut(s) | 329 |
| Hsp92II | CATG | 9 cut(s) | 101, 115, 310, 403, 472, 665, 722, 1469, 1594 |
| HspAI | GCGC | 1 cut(s) | 849 |
| Ksp22I | TGATCA | 2 cut(s) | 94, 402 |
| Kzo9I | GATC | 7 cut(s) | 94, 402, 617, 1114, 1267, 1518, 1544 |
| LmnI | GCTCC | 2 cut(s) | 237, 665 |
| Lsp1109I | GCAGC | 3 cut(s) | 179, 215, 1188 |
| LweI | GCATC | 4 cut(s) | 1006, 1424, 1463, 1611 |
| MaeI | CTAG | 6 cut(s) | 239, 311, 423, 686, 771, 1023 |
| MaeII | ACGT | 3 cut(s) | 535, 823, 1531 |
| MaeIII | GTNAC | 4 cut(s) | 531, 625, 804, 1077 |
| MalI | GATC | 7 cut(s) | 96, 404, 619, 1116, 1269, 1520, 1546 |
| MboI | GATC | 7 cut(s) | 94, 402, 617, 1114, 1267, 1518, 1544 |
| MboII | GAAGA | 6 cut(s) | 43, 161, 326, 365, 738, 1309 |
| MfeI | CAATTG | 1 cut(s) | 858 |
| MflI | RGATCY | 3 cut(s) | 617, 1267, 1544 |
| MhlI | GDGCHC | 2 cut(s) | 234, 1030 |
| MlsI | TGGCCA | 1 cut(s) | 365 |
| MluNI | TGGCCA | 1 cut(s) | 365 |
| MlyI | GAGTC | 2 cut(s) | 911, 1518 |
| MmeI | TCCRAC | 2 cut(s) | 154, 774 |
| Mox20I | TGGCCA | 1 cut(s) | 365 |
| MscI | TGGCCA | 1 cut(s) | 365 |
| MseI | TTAA | 6 cut(s) | 104, 602, 681, 1313, 1338, 1617 |
| MslI | CAYNNNNRTG | 3 cut(s) | 137, 1464, 1589 |
| Msp20I | TGGCCA | 1 cut(s) | 365 |
| MspA1I | CMGCKG | 3 cut(s) | 188, 335, 1264 |
| MspI | CCGG | 1 cut(s) | 852 |
| MspR9I | CCNGG | 5 cut(s) | 615, 853, 1173, 1356, 1566 |
| MunI | CAATTG | 1 cut(s) | 858 |
| MvaI | CCWGG | 4 cut(s) | 615, 1173, 1356, 1566 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 719, 1025, 1201 |
| NciI | CCSGG | 1 cut(s) | 853 |
| NcoI | CCATGG | 1 cut(s) | 1465 |
| NdeII | GATC | 7 cut(s) | 94, 402, 617, 1114, 1267, 1518, 1544 |
| NlaIII | CATG | 9 cut(s) | 101, 115, 310, 403, 472, 665, 722, 1469, 1594 |
| NlaIV | GGNNCC | 3 cut(s) | 1177, 1546, 1570 |
| NmuCI | GTSAC | 3 cut(s) | 531, 625, 804 |
| PagI | TCATGA | 2 cut(s) | 97, 1590 |
| PceI | AGGCCT | 2 cut(s) | 488, 815 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 939 |
| PfeI | GAWTC | 4 cut(s) | 292, 735, 777, 791 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 590, 1455 |
| PfoI | TCCNGGA | 2 cut(s) | 613, 1171 |
| PkrI | GCNGC | 3 cut(s) | 194, 205, 1203 |
| PleI | GAGTC | 2 cut(s) | 910, 1517 |
| PpsI | GAGTC | 2 cut(s) | 910, 1517 |
| Ppu21I | YACGTR | 2 cut(s) | 824, 1532 |
| PpuMI | RGGWCCY | 1 cut(s) | 1175 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1180 |
| Psp5II | RGGWCCY | 1 cut(s) | 1175 |
| Psp6I | CCWGG | 4 cut(s) | 613, 1171, 1354, 1564 |
| PspEI | GGTNACC | 1 cut(s) | 1077 |
| PspGI | CCWGG | 4 cut(s) | 613, 1171, 1354, 1564 |
| PspN4I | GGNNCC | 3 cut(s) | 1177, 1546, 1570 |
| PspPI | GGNCC | 5 cut(s) | 896, 1175, 1229, 1382, 1569 |
| PspPPI | RGGWCCY | 1 cut(s) | 1175 |
| PstI | CTGCAG | 1 cut(s) | 1206 |
| PsuI | RGATCY | 3 cut(s) | 617, 1267, 1544 |
| PvuII | CAGCTG | 1 cut(s) | 335 |
| RsaI | GTAC | 6 cut(s) | 41, 158, 184, 224, 634, 1163 |
| RsaNI | GTAC | 6 cut(s) | 40, 157, 183, 223, 633, 1162 |
| RseI | CAYNNNNRTG | 3 cut(s) | 137, 1464, 1589 |
| Rsr2I | CGGWCCG | 1 cut(s) | 1382 |
| RsrII | CGGWCCG | 1 cut(s) | 1382 |
| SaqAI | TTAA | 6 cut(s) | 104, 602, 681, 1313, 1338, 1617 |
| SatI | GCNGC | 3 cut(s) | 193, 204, 1202 |
| Sau3AI | GATC | 7 cut(s) | 94, 402, 617, 1114, 1267, 1518, 1544 |
| Sau96I | GGNCC | 5 cut(s) | 896, 1175, 1229, 1382, 1569 |
| ScaI | AGTACT | 1 cut(s) | 224 |
| SchI | GAGTC | 2 cut(s) | 911, 1518 |
| ScrFI | CCNGG | 5 cut(s) | 615, 853, 1173, 1356, 1566 |
| SduI | GDGCHC | 2 cut(s) | 234, 1030 |
| SfaNI | GCATC | 4 cut(s) | 1006, 1424, 1463, 1611 |
| SfcI | CTRYAG | 1 cut(s) | 1202 |
| SinI | GGWCC | 4 cut(s) | 1175, 1229, 1382, 1569 |
| SmiI | ATTTAAAT | 1 cut(s) | 603 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 137, 1464, 1589 |
| SmlI | CTYRAG | 2 cut(s) | 1344, 1578 |
| SmoI | CTYRAG | 2 cut(s) | 1344, 1578 |
| SseBI | AGGCCT | 2 cut(s) | 488, 815 |
| SsiI | CCGC | 5 cut(s) | 188, 1240, 1262, 1385, 1413 |
| SspMI | CTAG | 6 cut(s) | 239, 311, 423, 686, 771, 1023 |
| StuI | AGGCCT | 2 cut(s) | 488, 815 |
| StyD4I | CCNGG | 5 cut(s) | 613, 851, 1171, 1354, 1564 |
| StyI | CCWWGG | 1 cut(s) | 1465 |
| SwaI | ATTTAAAT | 1 cut(s) | 603 |
| TaaI | ACNGT | 2 cut(s) | 727, 1444 |
| TaiI | ACGT | 3 cut(s) | 538, 826, 1534 |
| TaqI | TCGA | 2 cut(s) | 642, 1435 |
| TaqII | GACCGA | 1 cut(s) | 1217 |
| TatI | WGTACW | 2 cut(s) | 222, 1161 |
| TfiI | GAWTC | 4 cut(s) | 292, 735, 777, 791 |
| Tru1I | TTAA | 6 cut(s) | 104, 602, 681, 1313, 1338, 1617 |
| Tru9I | TTAA | 6 cut(s) | 104, 602, 681, 1313, 1338, 1617 |
| TscAI | CASTG | 3 cut(s) | 139, 256, 732 |
| TseFI | GTSAC | 3 cut(s) | 531, 625, 804 |
| TseI | GCWGC | 3 cut(s) | 192, 203, 1201 |
| Tsp45I | GTSAC | 3 cut(s) | 531, 625, 804 |
| TspDTI | ATGAA | 5 cut(s) | 128, 707, 808, 903, 1412 |
| TspGWI | ACGGA | 2 cut(s) | 459, 1408 |
| TspRI | CASTG | 3 cut(s) | 139, 256, 732 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 590, 1455 |
| VneI | GTGCAC | 1 cut(s) | 1026 |
| VpaK11BI | GGWCC | 4 cut(s) | 1175, 1229, 1382, 1569 |
| XapI | RAATTY | 6 cut(s) | 116, 437, 1138, 1167, 1321, 1556 |
| XbaI | TCTAGA | 1 cut(s) | 422 |
| XspI | CTAG | 6 cut(s) | 239, 311, 423, 686, 771, 1023 |
| ZrmI | AGTACT | 1 cut(s) | 224 |