MD16G1265500.v1.1

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000533
Physical Location & Seq
Reverse (-)
33793039 .. 33794507
1469 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1265500.v1.1.491

Sequence Viewer

Length: 1038 bp
ATGCCTGGTACCATTTCAAATAGAATCACTGGCTCAACTTTTGACAATAATGGAACGAGACATGCAGCTGATGAACTACTTAACAAAAGAGACCTCCACAACTTGCGAGTTGCAGTTCATGCCAACGTAGAGAAGATCCTCTTCTCTTCTAGTGAATCAAGTACAAGTTATGTGCAGGGACAATGGGGACCCCACAATCTTGGAATCCTAGTTGTACTTGACCATCCATACGTTGGGCAGTTTCTGTACGACAATCCTCGTAATTTCATTAACATTTTGCCCCCAAATCCAATCGAAGCCTCAATCGTAACAGTTCTAGGCATTAGAAAAGATTTCTGGCAATGTTCTCTCTCCGGCGGACCACTTACTTCTCCGTCCTATAGTTTATTTCCTAGTGAGTCTCACCCCCTGCCAGTAAATTCAACTTTCGCTCACATTCCTAACAAAGTTCCTGGACCTTATTCTCATGGCTCTCTCACGCTAAATTCATCTTCTGATGTGACAATGGCCCCAATGACTAACGCATTTAAACCATATAAAACCCAAGATTTGCCAGGTATACAAGGTTTTACTTTTTTAGGAGTACCTCTGCCAAATAATCAAACAGATGATGCACTCGAAACATTTTGTCGAGATTCTTTGGGCTCATATTGGCATTACCACGGTGGATGCATTGATGGAAAGGTGGTTGATGGTGGTTTGCGTGTTAATGGAATTGATGCATTACGCATTGTTGATTCTTCCACATTCCCTGTCACACGGGCGAGCCACCCTATGGGCTTCTATCTGATGTTAGGCAGGTATATGGGTCTTCAAATTCTGCAAGAGAGATCAGCTTGGGTTGGTATATCATCCGCGTTATCCAACATTTCCAACGATATTATCCTTAATGATGCACTATCTTTTACTTCTCAATGCTTGATAATAAACCCCATTGTACATTGGGGATTAAGGTTTGGCTGCAAGAAGGGGACATGCTCTTTCTATGCCCCAGGCTTTCTGGTACCTCTTTATTTCATGTATAATATATTTCCTTAA

Protein Analysis

346

Amino Acids

37.92

Weight (kDa)

6.62

Isoelectric Point (pI)

47.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_C PF05199 196 - 266 1.7e-12 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 789
Acc65I GGTACC 2 cut(s) 8, 1003
AccB1I GGYRCC 2 cut(s) 8, 1003
AccB7I CCANNNNNTGG 2 cut(s) 233, 775
AccI GTMKAC 1 cut(s) 559
AccII CGCG 1 cut(s) 857
AciI CCGC 2 cut(s) 357, 855
AclWI GGATC 1 cut(s) 130
AcsI RAATTY 3 cut(s) 418, 484, 816
AfaI GTAC 7 cut(s) 10, 163, 216, 248, 585, 939, 1005
AfiI CCNNNNNNNGG 2 cut(s) 233, 775
AgsI TTSAA 3 cut(s) 18, 423, 815
AjnI CCWGG 4 cut(s) 4, 451, 553, 991
AluBI AGCT 2 cut(s) 68, 836
AluI AGCT 2 cut(s) 68, 836
Alw26I GTCTC 3 cut(s) 52, 84, 405
AlwI GGATC 1 cut(s) 130
AlwNI CAGNNNCTG 1 cut(s) 244
AoxI GGCC 1 cut(s) 507
ApeKI GCWGC 2 cut(s) 65, 960
ApoI RAATTY 3 cut(s) 418, 484, 816
Asp718I GGTACC 2 cut(s) 8, 1003
AspS9I GGNCC 4 cut(s) 188, 359, 455, 508
AsuHPI GGTGA 1 cut(s) 395
AvaII GGWCC 3 cut(s) 188, 359, 455
BanI GGYRCC 2 cut(s) 8, 1003
BanII GRGCYC 1 cut(s) 647
BbsI GAAGAC 1 cut(s) 803
BbvI GCAGC 2 cut(s) 77, 947
BccI CCATC 3 cut(s) 231, 671, 686
BciT130I CCWGG 4 cut(s) 6, 453, 555, 993
BcoDI GTCTC 3 cut(s) 52, 84, 405
BfaI CTAG 4 cut(s) 150, 209, 317, 393
BfmI CTRYAG 1 cut(s) 379
BfuAI ACCTGC 1 cut(s) 789
BisI GCNGC 2 cut(s) 66, 961
BlsI GCNGC 2 cut(s) 67, 962
Bme1390I CCNGG 4 cut(s) 6, 453, 555, 993
Bme18I GGWCC 3 cut(s) 188, 359, 455
BmgT120I GGNCC 4 cut(s) 188, 359, 455, 508
BmiI GGNNCC 5 cut(s) 10, 189, 190, 510, 1005
BmrFI CCNGG 4 cut(s) 6, 453, 555, 993
BmsI GCATC 4 cut(s) 601, 659, 709, 883
BpiI GAAGAC 1 cut(s) 803
BsaI GGTCTC 1 cut(s) 84
BsaJI CCNNGG 2 cut(s) 661, 991
Bsc4I CCNNNNNNNGG 2 cut(s) 233, 775
Bse1I ACTGG 2 cut(s) 34, 413
Bse3DI GCAATG 1 cut(s) 347
BseBI CCWGG 4 cut(s) 6, 453, 555, 993
BseDI CCNNGG 2 cut(s) 661, 991
BseGI GGATG 3 cut(s) 223, 674, 851
BseLI CCNNNNNNNGG 2 cut(s) 233, 775
BseMI GCAATG 1 cut(s) 347
BseNI ACTGG 2 cut(s) 34, 413
BseXI GCAGC 2 cut(s) 77, 947
BsgI GTGCAG 1 cut(s) 194
Bsh1236I CGCG 1 cut(s) 857
BshFI GGCC 1 cut(s) 509
BshNI GGYRCC 2 cut(s) 8, 1003
BsiSI CCGG 1 cut(s) 354
BslFI GGGAC 3 cut(s) 192, 201, 985
BslI CCNNNNNNNGG 2 cut(s) 233, 775
BsmAI GTCTC 3 cut(s) 52, 84, 405
BsmFI GGGAC 3 cut(s) 192, 201, 985
BsnI GGCC 1 cut(s) 509
Bso31I GGTCTC 1 cut(s) 84
Bsp1286I GDGCHC 1 cut(s) 647
Bsp1407I TGTACA 1 cut(s) 937
Bsp143I GATC 2 cut(s) 135, 830
BspACI CCGC 2 cut(s) 357, 855
BspANI GGCC 1 cut(s) 509
BspFNI CGCG 1 cut(s) 857
BspLI GGNNCC 5 cut(s) 10, 189, 190, 510, 1005
BspMI ACCTGC 1 cut(s) 789
BspPI GGATC 1 cut(s) 130
BspT107I GGYRCC 2 cut(s) 8, 1003
BspTNI GGTCTC 1 cut(s) 84
BsrDI GCAATG 1 cut(s) 347
BsrGI TGTACA 1 cut(s) 937
BsrI ACTGG 2 cut(s) 34, 413
BssECI CCNNGG 2 cut(s) 661, 991
BssMI GATC 2 cut(s) 135, 830
BssNAI GTATAC 1 cut(s) 560
Bst1107I GTATAC 1 cut(s) 560
Bst2UI CCWGG 4 cut(s) 6, 453, 555, 993
Bst4CI ACNGT 2 cut(s) 313, 665
Bst6I CTCTTC 2 cut(s) 146, 151
BstAPI GCANNNNNTGC 1 cut(s) 119
BstAUI TGTACA 1 cut(s) 937
BstC8I GCNNGC 1 cut(s) 766
BstDSI CCRYGG 1 cut(s) 661
BstF5I GGATG 3 cut(s) 223, 674, 851
BstFNI CGCG 1 cut(s) 857
BstKTI GATC 2 cut(s) 138, 833
BstMAI GTCTC 3 cut(s) 52, 84, 405
BstMBI GATC 2 cut(s) 135, 830
BstMWI GCNNNNNNNGC 1 cut(s) 119
BstNI CCWGG 4 cut(s) 6, 453, 555, 993
BstNSI RCATGY 2 cut(s) 65, 978
BstSCI CCNGG 4 cut(s) 4, 451, 553, 991
BstSFI CTRYAG 1 cut(s) 379
BstUI CGCG 1 cut(s) 857
BstV1I GCAGC 2 cut(s) 77, 947
BstV2I GAAGAC 1 cut(s) 803
BstX2I RGATCY 1 cut(s) 135
BstXI CCANNNNNNTGG 1 cut(s) 200
BstYI RGATCY 1 cut(s) 135
BstZ17I GTATAC 1 cut(s) 560
BsuRI GGCC 1 cut(s) 509
BtgI CCRYGG 1 cut(s) 661
BtsCI GGATG 3 cut(s) 223, 674, 851
BtsIMutI CAGTG 1 cut(s) 27
BveI ACCTGC 1 cut(s) 789
Cac8I GCNNGC 1 cut(s) 766
CaiI CAGNNNCTG 1 cut(s) 244
Cfr13I GGNCC 4 cut(s) 188, 359, 455, 508
Csp6I GTAC 7 cut(s) 9, 162, 215, 247, 584, 938, 1004
CviAII CATG 5 cut(s) 62, 119, 467, 975, 1018
CviQI GTAC 7 cut(s) 9, 162, 215, 247, 584, 938, 1004
DpnI GATC 2 cut(s) 137, 832
DpnII GATC 2 cut(s) 135, 830
DraI TTTAAA 1 cut(s) 529
Eam1104I CTCTTC 2 cut(s) 146, 151
EarI CTCTTC 2 cut(s) 146, 151
EciI GGCGGA 1 cut(s) 372
Eco24I GRGCYC 1 cut(s) 647
Eco31I GGTCTC 1 cut(s) 84
Eco47I GGWCC 3 cut(s) 188, 359, 455
EcoO109I RGGNCCY 1 cut(s) 188
EcoRII CCWGG 4 cut(s) 4, 451, 553, 991
EcoT22I ATGCAT 2 cut(s) 674, 724
EcoT38I GRGCYC 1 cut(s) 647
FaeI CATG 5 cut(s) 65, 122, 470, 978, 1021
FalI AAGNNNNNCTT 2 cut(s) 125, 157
FaqI GGGAC 3 cut(s) 192, 201, 985
FatI CATG 5 cut(s) 61, 118, 466, 974, 1017
FblI GTMKAC 1 cut(s) 559
Fnu4HI GCNGC 2 cut(s) 66, 961
FokI GGATG 3 cut(s) 210, 681, 838
FriOI GRGCYC 1 cut(s) 647
Fsp4HI GCNGC 2 cut(s) 66, 961
FspBI CTAG 4 cut(s) 150, 209, 317, 393
GluI GCNGC 2 cut(s) 66, 961
HaeIII GGCC 1 cut(s) 509
HapII CCGG 1 cut(s) 354
Hin1II CATG 5 cut(s) 65, 122, 470, 978, 1021
HinfI GANTC 6 cut(s) 24, 155, 204, 398, 635, 737
HpaII CCGG 1 cut(s) 354
HphI GGTGA 1 cut(s) 395
Hpy166II GTNNAC 1 cut(s) 560
Hpy188I TCNGA 2 cut(s) 496, 789
Hpy188III TCNNGA 1 cut(s) 632
Hpy8I GTNNAC 1 cut(s) 560
HpyAV CCTTC 1 cut(s) 961
HpyCH4III ACNGT 2 cut(s) 313, 665
HpyCH4IV ACGT 2 cut(s) 126, 231
HpyCH4V TGCA 9 cut(s) 65, 113, 175, 614, 672, 722, 823, 896, 963
HpyF10VI GCNNNNNNNGC 1 cut(s) 119
HpySE526I ACGT 2 cut(s) 126, 231
Hsp92II CATG 5 cut(s) 65, 122, 470, 978, 1021
KflI GGGWCCC 1 cut(s) 188
KpnI GGTACC 2 cut(s) 12, 1007
Kzo9I GATC 2 cut(s) 135, 830
Lsp1109I GCAGC 2 cut(s) 77, 947
LweI GCATC 4 cut(s) 601, 659, 709, 883
MaeI CTAG 4 cut(s) 150, 209, 317, 393
MaeII ACGT 2 cut(s) 126, 231
MaeIII GTNAC 3 cut(s) 307, 499, 754
MalI GATC 2 cut(s) 137, 832
MboI GATC 2 cut(s) 135, 830
MboII GAAGA 6 cut(s) 133, 138, 145, 483, 732, 803
MflI RGATCY 1 cut(s) 135
MhlI GDGCHC 1 cut(s) 647
MluCI AATT 5 cut(s) 262, 418, 484, 714, 816
MlyI GAGTC 1 cut(s) 407
MmeI TCCRAC 2 cut(s) 888, 897
MnlI CCTC 6 cut(s) 104, 149, 267, 310, 597, 1017
Mph1103I ATGCAT 2 cut(s) 674, 724
MseI TTAA 7 cut(s) 81, 270, 528, 708, 888, 950, 1036
MspA1I CMGCKG 1 cut(s) 68
MspI CCGG 1 cut(s) 354
MspR9I CCNGG 4 cut(s) 6, 453, 555, 993
MvaI CCWGG 4 cut(s) 6, 453, 555, 993
MvnI CGCG 1 cut(s) 857
MwoI GCNNNNNNNGC 1 cut(s) 119
NdeII GATC 2 cut(s) 135, 830
NlaIII CATG 5 cut(s) 65, 122, 470, 978, 1021
NlaIV GGNNCC 5 cut(s) 10, 189, 190, 510, 1005
NmuCI GTSAC 2 cut(s) 499, 754
NsiI ATGCAT 2 cut(s) 674, 724
NspI RCATGY 2 cut(s) 65, 978
PfeI GAWTC 5 cut(s) 24, 155, 204, 635, 737
PflMI CCANNNNNTGG 2 cut(s) 233, 775
PfoI TCCNGGA 1 cut(s) 451
PkrI GCNGC 2 cut(s) 67, 962
PleI GAGTC 1 cut(s) 406
PpsI GAGTC 1 cut(s) 406
PpuMI RGGWCCY 1 cut(s) 188
Psp5II RGGWCCY 1 cut(s) 188
Psp6I CCWGG 4 cut(s) 4, 451, 553, 991
PspGI CCWGG 4 cut(s) 4, 451, 553, 991
PspN4I GGNNCC 5 cut(s) 10, 189, 190, 510, 1005
PspPI GGNCC 4 cut(s) 188, 359, 455, 508
PspPPI RGGWCCY 1 cut(s) 188
PstNI CAGNNNCTG 1 cut(s) 244
PsuI RGATCY 1 cut(s) 135
PvuII CAGCTG 1 cut(s) 68
RsaI GTAC 7 cut(s) 10, 163, 216, 248, 585, 939, 1005
RsaNI GTAC 7 cut(s) 9, 162, 215, 247, 584, 938, 1004
SaqAI TTAA 7 cut(s) 81, 270, 528, 708, 888, 950, 1036
SatI GCNGC 2 cut(s) 66, 961
Sau3AI GATC 2 cut(s) 135, 830
Sau96I GGNCC 4 cut(s) 188, 359, 455, 508
SchI GAGTC 1 cut(s) 407
ScrFI CCNGG 4 cut(s) 6, 453, 555, 993
SduI GDGCHC 1 cut(s) 647
SfaNI GCATC 4 cut(s) 601, 659, 709, 883
SfcI CTRYAG 1 cut(s) 379
SinI GGWCC 3 cut(s) 188, 359, 455
Sse9I AATT 5 cut(s) 262, 418, 484, 714, 816
SsiI CCGC 2 cut(s) 357, 855
SspMI CTAG 4 cut(s) 150, 209, 317, 393
StyD4I CCNGG 4 cut(s) 4, 451, 553, 991
TaaI ACNGT 2 cut(s) 313, 665
TaiI ACGT 2 cut(s) 129, 234
TaqI TCGA 3 cut(s) 294, 618, 631
TasI AATT 5 cut(s) 262, 418, 484, 714, 816
TatI WGTACW 3 cut(s) 161, 214, 937
TfiI GAWTC 5 cut(s) 24, 155, 204, 635, 737
Tru1I TTAA 7 cut(s) 81, 270, 528, 708, 888, 950, 1036
Tru9I TTAA 7 cut(s) 81, 270, 528, 708, 888, 950, 1036
TscAI CASTG 1 cut(s) 34
TseFI GTSAC 2 cut(s) 499, 754
TseI GCWGC 2 cut(s) 65, 960
Tsp45I GTSAC 2 cut(s) 499, 754
TspDTI ATGAA 5 cut(s) 87, 107, 256, 477, 1006
TspGWI ACGGA 1 cut(s) 363
TspRI CASTG 1 cut(s) 34
Van91I CCANNNNNTGG 2 cut(s) 233, 775
VpaK11BI GGWCC 3 cut(s) 188, 359, 455
XapI RAATTY 3 cut(s) 418, 484, 816
XceI RCATGY 2 cut(s) 65, 978
XcmI CCANNNNNNNNNTGG 1 cut(s) 230
XmiI GTMKAC 1 cut(s) 559
XspI CTAG 4 cut(s) 150, 209, 317, 393
Zsp2I ATGCAT 2 cut(s) 674, 724
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.