Prupe.1G092400_v2.0.a1

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000533
Physical Location & Seq
Reverse (-)
7009921 .. 7012260
2340 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G092400.1

Sequence Viewer

Length: 1653 bp
ATGGTGAAATCAACAATGTCAGCTATACTAGTATTGGTGCTGCACCTTTTTGTTCTTCATCTTCAATATTCAGAGATTCATTCGCTTGCCAATACCTCTGCTCATGATTTTAGCTACTTGGAAATTGTTTACGATGCCAATGACACAGAGTTGGAAGGAACATACGACTACATTATCGTTGGTGGAGGAACAGCAGGGTGTCCATTGGCAGCAACTTTGTCAGCAAACTACTCGGTGCTTGTTCTGGAAAGGGGCACTCTTCCTACAGAATATCCAAACCTGTTGACTTCAGATGGGTTTATATATAATCTGCAGCAAGAAGATGATGGACAGACACCAGTCGAAAGGTTCGTATCTGGAGATGGTATTGACAATGTAAGAGGGAGGGTCCTCGGTGGCACGAGCATGATCAATGCCGGGGTCTACGTAAGAGCTAACACCTCGTTCTTTAATCAAACAGGGATTGAATGGGACATGGATTTGGTTAATAAGACATATGACTGGGTTGAAGACACTATTGTGTTCAAGCCGGATTTCCAATTTTGGCAAAATCTTACACGAACTGCGTTCTTGGAGGTTGGTATTCTTCCAGACAATGGATTTAGTTTGGATCACATAGAAGGAACTAGACTCACCGGCTCAACTTTCGACAATAATGGAACCAGACATGCATCTGATGAACTGCTTAATAAAGGAGATCCAAACAACTTGCGAGTTGCAGTTCATGCCGCAGTAGAGAAGATCATCTTCTCTTCCAATTCATCAGGTGTGACAGCTATAGGAGTTATATATACTGATTCGAACGGAACGACTCATCAGGCATTTGTACGTGGTGAGGGAGAAGTTATATTGAGTGCAGGGCCAATTGGGTCCCCTCAACTTCTACTACTTAGTGGTGTTGGCCCAGAGTCTTACCTAACATCTCTCAACATTTCAGTTGTTGCTTCCCATCCATACGTCGGGCAGTATATATATGACAATCCTCGTAATTTCATTAACATTTTGCCCCCAAATCCAATAGAACCCTCAACTGTCACCGTTCTAGGCATTACAAGCGACTTCTACCAATGTTCTCTATCAAGCTTGCCATTTTCTATTGCACCCTTTAGTTTTTTTCCTAATCCAACTTATCCCCTGCCAAAAACGACTTTCGCTCACATTGTTAACAAAGTTCCGGGACCATTATCTTATGGTACTGTCACGCTGCAATCAACCTCTGACGTGAGAGTCGCTCCAAATGTCAAATTCAACTACTATTCGAATTCAACTGACCTTGCTCATTGTGTTAGCGGCATGAAGAAGATTGGTGAATTCTTGAGCTCAGACGCATTAAAGCCATATAAAGTGGAAGATTTGCCGGGCATAGAAGGTTTTGATATTTTGGGTATACCTTTGCCAGAGGACCAGACAGATGATGCAGCCTTCGAAACATTTTGCCAGGTGGCAGTAGCGTCATATTGGCATTACCACGGTGGATGCCTTGTCGGGGAGGTGCTTGATGATGATTTCCGTGTGACAGGGATCAACGCATTACGCGTTGTTGATGGCTCCACATTCCCTTCCACACCAGCAAGCCATCCACAGGGCTTCTATCTGATGTTAGGGAGGTACGTGGGCTCTAAAATTCTGCAAGAAAGATTAGCTTCAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko01110 map00460 map01110
Pfam Domains
Protein Families
GMC_oxred_C GMC_oxred_N

Protein Analysis

551

Amino Acids

59.95

Weight (kDa)

4.64

Isoelectric Point (pI)

34.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1226
AccB7I CCANNNNNTGG 1 cut(s) 596
AccI GTMKAC 2 cut(s) 423, 1387
AccII CGCG 1 cut(s) 1536
AciI CCGC 2 cut(s) 729, 1290
AclWI GGATC 3 cut(s) 618, 692, 1529
AcsI RAATTY 4 cut(s) 1244, 1261, 1310, 1623
AcuI CTGAAG 2 cut(s) 273, 1629
AfaI GTAC 3 cut(s) 828, 1195, 1610
AfiI CCNNNNNNNGG 4 cut(s) 596, 959, 1486, 1582
AflIII ACRYGT 1 cut(s) 1534
AgsI TTSAA 6 cut(s) 65, 467, 509, 526, 1249, 1266
AhlI ACTAGT 1 cut(s) 28
AjiI CACGTC 1 cut(s) 1222
AjnI CCWGG 1 cut(s) 1437
AleI CACNNNNGTG 1 cut(s) 518
AluBI AGCT 7 cut(s) 23, 114, 434, 776, 1083, 1320, 1643
AluI AGCT 7 cut(s) 23, 114, 434, 776, 1083, 1320, 1643
Alw21I GWGCWC 1 cut(s) 1322
AlwI GGATC 3 cut(s) 618, 692, 1529
AoxI GGCC 2 cut(s) 860, 901
ApeKI GCWGC 5 cut(s) 40, 209, 313, 1204, 1418
ApoI RAATTY 4 cut(s) 1244, 1261, 1310, 1623
AspS9I GGNCC 6 cut(s) 388, 860, 870, 902, 1178, 1402
AsuC2I CCSGG 3 cut(s) 418, 1176, 1359
AsuHPI GGTGA 5 cut(s) 16, 625, 845, 1027, 1319
AsuII TTCGAA 3 cut(s) 800, 1259, 1425
AvaII GGWCC 4 cut(s) 388, 870, 1178, 1402
BaeGI GKGCMC 1 cut(s) 257
BanII GRGCYC 2 cut(s) 1322, 1619
BauI CACGAG 1 cut(s) 400
BbsI GAAGAC 1 cut(s) 516
Bbv12I GWGCWC 1 cut(s) 1322
BbvI GCAGC 5 cut(s) 27, 221, 325, 1191, 1430
BccI CCATC 6 cut(s) 287, 320, 356, 957, 1538, 1584
BcgI CGANNNNNNTGC 2 cut(s) 213, 247
BciT130I CCWGG 1 cut(s) 1439
BclI TGATCA 1 cut(s) 408
BcnI CCSGG 3 cut(s) 418, 1176, 1359
BcuI ACTAGT 1 cut(s) 28
BfaI CTAG 3 cut(s) 29, 627, 1043
BfmI CTRYAG 3 cut(s) 264, 311, 777
BisI GCNGC 7 cut(s) 41, 210, 314, 729, 1205, 1291, 1419
BlsI GCNGC 7 cut(s) 42, 211, 315, 730, 1206, 1292, 1420
Bme1390I CCNGG 4 cut(s) 418, 1176, 1359, 1439
Bme18I GGWCC 4 cut(s) 388, 870, 1178, 1402
BmgBI CACGTC 1 cut(s) 1222
BmgT120I GGNCC 6 cut(s) 388, 860, 870, 902, 1178, 1402
BmiI GGNNCC 6 cut(s) 389, 661, 871, 872, 1179, 1549
BmrFI CCNGG 4 cut(s) 418, 1176, 1359, 1439
BmrI ACTGGG 1 cut(s) 511
BmsI GCATC 4 cut(s) 124, 680, 1405, 1466
BmuI ACTGGG 1 cut(s) 511
BoxI GACNNNNGTC 1 cut(s) 338
BpiI GAAGAC 1 cut(s) 516
BplI GAGNNNNNCTC 2 cut(s) 1216, 1248
BpmI CTGGAG 1 cut(s) 378
Bpu14I TTCGAA 3 cut(s) 800, 1259, 1425
BpuEI CTTGAG 1 cut(s) 1336
BpuMI CCSGG 3 cut(s) 418, 1176, 1359
BsaAI YACGTR 3 cut(s) 427, 830, 1612
BsaJI CCNNGG 3 cut(s) 391, 417, 1468
Bsc4I CCNNNNNNNGG 4 cut(s) 596, 959, 1486, 1582
Bse118I RCCGGY 1 cut(s) 635
Bse1I ACTGG 2 cut(s) 338, 506
BseBI CCWGG 1 cut(s) 1439
BseDI CCNNGG 3 cut(s) 391, 417, 1468
BseGI GGATG 3 cut(s) 949, 1481, 1576
BseLI CCNNNNNNNGG 4 cut(s) 596, 959, 1486, 1582
BseMII CTCAG 1 cut(s) 1335
BseNI ACTGG 2 cut(s) 338, 506
BseSI GKGCMC 1 cut(s) 257
BseXI GCAGC 5 cut(s) 27, 221, 325, 1191, 1430
BsgI GTGCAG 2 cut(s) 26, 876
Bsh1236I CGCG 1 cut(s) 1536
BshFI GGCC 2 cut(s) 862, 903
BsiHKAI GWGCWC 1 cut(s) 1322
BsiSI CCGG 5 cut(s) 417, 530, 636, 1175, 1358
BslFI GGGAC 3 cut(s) 485, 856, 1191
BslI CCNNNNNNNGG 4 cut(s) 596, 959, 1486, 1582
BsmFI GGGAC 3 cut(s) 485, 856, 1191
BsnI GGCC 2 cut(s) 862, 903
Bsp119I TTCGAA 3 cut(s) 800, 1259, 1425
Bsp1286I GDGCHC 3 cut(s) 257, 1322, 1619
Bsp143I GATC 5 cut(s) 408, 610, 697, 741, 1521
BspACI CCGC 2 cut(s) 729, 1290
BspANI GGCC 2 cut(s) 862, 903
BspCNI CTCAG 1 cut(s) 1334
BspFNI CGCG 1 cut(s) 1536
BspHI TCATGA 1 cut(s) 103
BspLI GGNNCC 6 cut(s) 389, 661, 871, 872, 1179, 1549
BspMAI CTGCAG 1 cut(s) 315
BspPI GGATC 3 cut(s) 618, 692, 1529
BspT104I TTCGAA 3 cut(s) 800, 1259, 1425
BsrFI RCCGGY 1 cut(s) 635
BsrI ACTGG 2 cut(s) 338, 506
BssAI RCCGGY 1 cut(s) 635
BssECI CCNNGG 3 cut(s) 391, 417, 1468
BssMI GATC 5 cut(s) 408, 610, 697, 741, 1521
BssNAI GTATAC 1 cut(s) 1388
BssSI CACGAG 1 cut(s) 400
Bst1107I GTATAC 1 cut(s) 1388
Bst2BI CACGAG 1 cut(s) 400
Bst2UI CCWGG 1 cut(s) 1439
Bst4CI ACNGT 4 cut(s) 1033, 1039, 1198, 1472
Bst6I CTCTTC 2 cut(s) 264, 757
BstAPI GCANNNNNTGC 1 cut(s) 725
BstBAI YACGTR 3 cut(s) 427, 830, 1612
BstBI TTCGAA 3 cut(s) 800, 1259, 1425
BstC8I GCNNGC 3 cut(s) 87, 1085, 1573
BstDEI CTNAG 2 cut(s) 890, 1321
BstDSI CCRYGG 1 cut(s) 1468
BstF5I GGATG 3 cut(s) 949, 1481, 1576
BstFNI CGCG 1 cut(s) 1536
BstKTI GATC 5 cut(s) 411, 613, 700, 744, 1524
BstMBI GATC 5 cut(s) 408, 610, 697, 741, 1521
BstMWI GCNNNNNNNGC 2 cut(s) 725, 1053
BstNI CCWGG 1 cut(s) 1439
BstNSI RCATGY 1 cut(s) 671
BstPAI GACNNNNGTC 1 cut(s) 338
BstSCI CCNGG 4 cut(s) 416, 1174, 1357, 1437
BstSFI CTRYAG 3 cut(s) 264, 311, 777
BstSLI GKGCMC 1 cut(s) 257
BstSNI TACGTA 1 cut(s) 427
BstUI CGCG 1 cut(s) 1536
BstV1I GCAGC 5 cut(s) 27, 221, 325, 1191, 1430
BstV2I GAAGAC 1 cut(s) 516
BstX2I RGATCY 1 cut(s) 697
BstYI RGATCY 1 cut(s) 697
BstZ17I GTATAC 1 cut(s) 1388
BsuRI GGCC 2 cut(s) 862, 903
BtgI CCRYGG 1 cut(s) 1468
BtrI CACGTC 1 cut(s) 1222
BtsCI GGATG 3 cut(s) 949, 1481, 1576
Cac8I GCNNGC 3 cut(s) 87, 1085, 1573
CciI TCATGA 1 cut(s) 103
Cfr10I RCCGGY 1 cut(s) 635
Cfr13I GGNCC 6 cut(s) 388, 860, 870, 902, 1178, 1402
CseI GACGC 2 cut(s) 1334, 1440
Csp6I GTAC 3 cut(s) 827, 1194, 1609
CviAII CATG 6 cut(s) 104, 406, 475, 668, 725, 1294
CviQI GTAC 3 cut(s) 827, 1194, 1609
DdeI CTNAG 2 cut(s) 890, 1321
DpnI GATC 5 cut(s) 410, 612, 699, 743, 1523
DpnII GATC 5 cut(s) 408, 610, 697, 741, 1521
DrdI GACNNNNNNGTC 1 cut(s) 1226
DseDI GACNNNNNNGTC 1 cut(s) 1226
Eam1104I CTCTTC 2 cut(s) 264, 757
EarI CTCTTC 2 cut(s) 264, 757
Ecl136II GAGCTC 1 cut(s) 1320
Eco105I TACGTA 1 cut(s) 427
Eco24I GRGCYC 2 cut(s) 1322, 1619
Eco47I GGWCC 4 cut(s) 388, 870, 1178, 1402
Eco53kI GAGCTC 1 cut(s) 1320
Eco57I CTGAAG 2 cut(s) 273, 1629
EcoICRI GAGCTC 1 cut(s) 1320
EcoO109I RGGNCCY 2 cut(s) 388, 870
EcoRI GAATTC 2 cut(s) 1261, 1310
EcoRII CCWGG 1 cut(s) 1437
EcoT22I ATGCAT 1 cut(s) 673
EcoT38I GRGCYC 2 cut(s) 1322, 1619
FaeI CATG 6 cut(s) 107, 409, 478, 671, 728, 1297
FalI AAGNNNNNCTT 3 cut(s) 731, 763, 1627
FaqI GGGAC 3 cut(s) 485, 856, 1191
FatI CATG 6 cut(s) 103, 405, 474, 667, 724, 1293
FauNDI CATATG 1 cut(s) 496
FbaI TGATCA 1 cut(s) 408
FblI GTMKAC 2 cut(s) 423, 1387
Fnu4HI GCNGC 7 cut(s) 41, 210, 314, 729, 1205, 1291, 1419
FokI GGATG 3 cut(s) 936, 1488, 1563
FriOI GRGCYC 2 cut(s) 1322, 1619
Fsp4HI GCNGC 7 cut(s) 41, 210, 314, 729, 1205, 1291, 1419
FspBI CTAG 3 cut(s) 29, 627, 1043
GluI GCNGC 7 cut(s) 41, 210, 314, 729, 1205, 1291, 1419
GsuI CTGGAG 1 cut(s) 378
HaeIII GGCC 2 cut(s) 862, 903
HapII CCGG 5 cut(s) 417, 530, 636, 1175, 1358
HgaI GACGC 2 cut(s) 1334, 1440
Hin1II CATG 6 cut(s) 107, 409, 478, 671, 728, 1297
HincII GTYRAC 2 cut(s) 285, 1165
HindII GTYRAC 2 cut(s) 285, 1165
HindIII AAGCTT 1 cut(s) 1081
HinfI GANTC 6 cut(s) 76, 630, 797, 811, 908, 1227
HpaI GTTAAC 1 cut(s) 1165
HpaII CCGG 5 cut(s) 417, 530, 636, 1175, 1358
HphI GGTGA 5 cut(s) 16, 625, 845, 1027, 1319
Hpy166II GTNNAC 5 cut(s) 130, 285, 424, 1165, 1388
Hpy188I TCNGA 7 cut(s) 73, 292, 676, 1219, 1324, 1596, 1648
Hpy188III TCNNGA 5 cut(s) 104, 245, 357, 590, 1315
Hpy8I GTNNAC 5 cut(s) 130, 285, 424, 1165, 1388
Hpy99I CGWCG 1 cut(s) 962
HpyAV CCTTC 5 cut(s) 149, 614, 1361, 1432, 1569
HpyCH4III ACNGT 4 cut(s) 1033, 1039, 1198, 1472
HpyCH4IV ACGT 5 cut(s) 426, 829, 957, 1221, 1611
HpyCH4V TGCA 9 cut(s) 43, 313, 671, 719, 857, 1100, 1207, 1418, 1630
HpyF10VI GCNNNNNNNGC 2 cut(s) 725, 1053
HpyF3I CTNAG 2 cut(s) 890, 1321
HpySE526I ACGT 5 cut(s) 426, 829, 957, 1221, 1611
Hsp92II CATG 6 cut(s) 107, 409, 478, 671, 728, 1297
KflI GGGWCCC 1 cut(s) 870
Ksp22I TGATCA 1 cut(s) 408
KspAI GTTAAC 1 cut(s) 1165
Kzo9I GATC 5 cut(s) 408, 610, 697, 741, 1521
LmnI GCTCC 2 cut(s) 1237, 1553
Lsp1109I GCAGC 5 cut(s) 27, 221, 325, 1191, 1430
LweI GCATC 4 cut(s) 124, 680, 1405, 1466
MaeI CTAG 3 cut(s) 29, 627, 1043
MaeII ACGT 5 cut(s) 426, 829, 957, 1221, 1611
MaeIII GTNAC 4 cut(s) 769, 1033, 1198, 1513
MalI GATC 5 cut(s) 410, 612, 699, 743, 1523
MboI GATC 5 cut(s) 408, 610, 697, 741, 1521
MfeI CAATTG 1 cut(s) 864
MflI RGATCY 1 cut(s) 697
MhlI GDGCHC 3 cut(s) 257, 1322, 1619
MluCI AATT 9 cut(s) 123, 539, 757, 864, 988, 1244, 1261, 1310, 1623
MluI ACGCGT 1 cut(s) 1534
MlyI GAGTC 4 cut(s) 624, 805, 917, 1236
MmeI TCCRAC 2 cut(s) 132, 1148
Mph1103I ATGCAT 1 cut(s) 673
MseI TTAA 6 cut(s) 450, 486, 687, 996, 1164, 1331
MslI CAYNNNNRTG 2 cut(s) 404, 518
MspI CCGG 5 cut(s) 417, 530, 636, 1175, 1358
MspR9I CCNGG 4 cut(s) 418, 1176, 1359, 1439
MunI CAATTG 1 cut(s) 864
MvaI CCWGG 1 cut(s) 1439
MvnI CGCG 1 cut(s) 1536
MwoI GCNNNNNNNGC 2 cut(s) 725, 1053
NciI CCSGG 3 cut(s) 418, 1176, 1359
NdeI CATATG 1 cut(s) 496
NdeII GATC 5 cut(s) 408, 610, 697, 741, 1521
NlaIII CATG 6 cut(s) 107, 409, 478, 671, 728, 1297
NlaIV GGNNCC 6 cut(s) 389, 661, 871, 872, 1179, 1549
NmuCI GTSAC 4 cut(s) 769, 1033, 1198, 1513
NsiI ATGCAT 1 cut(s) 673
NspI RCATGY 1 cut(s) 671
NspV TTCGAA 3 cut(s) 800, 1259, 1425
OliI CACNNNNGTG 1 cut(s) 518
PagI TCATGA 1 cut(s) 103
PcsI WCGNNNNNNNCGW 3 cut(s) 348, 806, 1533
PfeI GAWTC 2 cut(s) 76, 797
PflMI CCANNNNNTGG 1 cut(s) 596
PfoI TCCNGGA 1 cut(s) 1174
PkrI GCNGC 7 cut(s) 42, 211, 315, 730, 1206, 1292, 1420
PleI GAGTC 4 cut(s) 624, 805, 916, 1235
PpsI GAGTC 4 cut(s) 624, 805, 916, 1235
Ppu21I YACGTR 3 cut(s) 427, 830, 1612
PpuMI RGGWCCY 2 cut(s) 388, 870
PshAI GACNNNNGTC 1 cut(s) 338
Psp124BI GAGCTC 1 cut(s) 1322
Psp5II RGGWCCY 2 cut(s) 388, 870
Psp6I CCWGG 1 cut(s) 1437
PspGI CCWGG 1 cut(s) 1437
PspN4I GGNNCC 6 cut(s) 389, 661, 871, 872, 1179, 1549
PspPI GGNCC 6 cut(s) 388, 860, 870, 902, 1178, 1402
PspPPI RGGWCCY 2 cut(s) 388, 870
PstI CTGCAG 1 cut(s) 315
PsuI RGATCY 1 cut(s) 697
RsaI GTAC 3 cut(s) 828, 1195, 1610
RsaNI GTAC 3 cut(s) 827, 1194, 1609
RseI CAYNNNNRTG 2 cut(s) 404, 518
SacI GAGCTC 1 cut(s) 1322
SaqAI TTAA 6 cut(s) 450, 486, 687, 996, 1164, 1331
SatI GCNGC 7 cut(s) 41, 210, 314, 729, 1205, 1291, 1419
Sau3AI GATC 5 cut(s) 408, 610, 697, 741, 1521
Sau96I GGNCC 6 cut(s) 388, 860, 870, 902, 1178, 1402
SchI GAGTC 4 cut(s) 624, 805, 917, 1236
ScrFI CCNGG 4 cut(s) 418, 1176, 1359, 1439
SduI GDGCHC 3 cut(s) 257, 1322, 1619
SfaNI GCATC 4 cut(s) 124, 680, 1405, 1466
SfcI CTRYAG 3 cut(s) 264, 311, 777
SfuI TTCGAA 3 cut(s) 800, 1259, 1425
SinI GGWCC 4 cut(s) 388, 870, 1178, 1402
SmiMI CAYNNNNRTG 2 cut(s) 404, 518
SmlI CTYRAG 1 cut(s) 1315
SmoI CTYRAG 1 cut(s) 1315
SnaBI TACGTA 1 cut(s) 427
SpeI ACTAGT 1 cut(s) 28
Sse9I AATT 9 cut(s) 123, 539, 757, 864, 988, 1244, 1261, 1310, 1623
SsiI CCGC 2 cut(s) 729, 1290
SspI AATATT 1 cut(s) 68
SspMI CTAG 3 cut(s) 29, 627, 1043
SstI GAGCTC 1 cut(s) 1322
StyD4I CCNGG 4 cut(s) 416, 1174, 1357, 1437
TaaI ACNGT 4 cut(s) 1033, 1039, 1198, 1472
TaiI ACGT 5 cut(s) 429, 832, 960, 1224, 1614
TaqI TCGA 5 cut(s) 342, 648, 800, 1259, 1425
TasI AATT 9 cut(s) 123, 539, 757, 864, 988, 1244, 1261, 1310, 1623
TauI GCSGC 2 cut(s) 731, 1293
TfiI GAWTC 2 cut(s) 76, 797
Tru1I TTAA 6 cut(s) 450, 486, 687, 996, 1164, 1331
Tru9I TTAA 6 cut(s) 450, 486, 687, 996, 1164, 1331
TseFI GTSAC 4 cut(s) 769, 1033, 1198, 1513
TseI GCWGC 5 cut(s) 40, 209, 313, 1204, 1418
Tsp45I GTSAC 4 cut(s) 769, 1033, 1198, 1513
TspDTI ATGAA 7 cut(s) 47, 68, 693, 713, 750, 982, 1310
TspGWI ACGGA 2 cut(s) 819, 1499
Van91I CCANNNNNTGG 1 cut(s) 596
VpaK11BI GGWCC 4 cut(s) 388, 870, 1178, 1402
XapI RAATTY 4 cut(s) 1244, 1261, 1310, 1623
XceI RCATGY 1 cut(s) 671
XmiI GTMKAC 2 cut(s) 423, 1387
XspI CTAG 3 cut(s) 29, 627, 1043
Zsp2I ATGCAT 1 cut(s) 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.