(R)-mandelonitrile lyase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 593 |
| AccI | GTMKAC | 1 cut(s) | 420 |
| AccII | CGCG | 2 cut(s) | 825, 1530 |
| AciI | CCGC | 4 cut(s) | 180, 825, 888, 1284 |
| AclWI | GGATC | 2 cut(s) | 163, 1523 |
| AcsI | RAATTY | 3 cut(s) | 1135, 1238, 1617 |
| AcuI | CTGAAG | 1 cut(s) | 1623 |
| AcyI | GRCGYC | 1 cut(s) | 132 |
| AfaI | GTAC | 4 cut(s) | 375, 521, 822, 1117 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 593, 953, 1100, 1452, 1576 |
| AflIII | ACRYGT | 1 cut(s) | 1528 |
| AgeI | ACCGGT | 1 cut(s) | 334 |
| AgsI | TTSAA | 6 cut(s) | 62, 1016, 1047, 1243, 1254, 1343 |
| AjnI | CCWGG | 1 cut(s) | 757 |
| AleI | CACNNNNGTG | 1 cut(s) | 515 |
| AluBI | AGCT | 6 cut(s) | 23, 111, 671, 770, 1077, 1637 |
| AluI | AGCT | 6 cut(s) | 23, 111, 671, 770, 1077, 1637 |
| Alw21I | GWGCWC | 3 cut(s) | 237, 390, 1316 |
| Alw26I | GTCTC | 1 cut(s) | 655 |
| AlwI | GGATC | 2 cut(s) | 163, 1523 |
| Ama87I | CYCGRG | 1 cut(s) | 1072 |
| AoxI | GGCC | 1 cut(s) | 896 |
| ApeKI | GCWGC | 4 cut(s) | 206, 668, 1412, 1673 |
| ApoI | RAATTY | 3 cut(s) | 1135, 1238, 1617 |
| AsiGI | ACCGGT | 1 cut(s) | 334 |
| AspS9I | GGNCC | 5 cut(s) | 73, 864, 896, 1172, 1396 |
| AsuC2I | CCSGG | 1 cut(s) | 1353 |
| AsuHPI | GGTGA | 3 cut(s) | 800, 1313, 1508 |
| AsuII | TTCGAA | 1 cut(s) | 1419 |
| AsuNHI | GCTAGC | 1 cut(s) | 1157 |
| AvaI | CYCGRG | 1 cut(s) | 1072 |
| AvaII | GGWCC | 4 cut(s) | 73, 864, 1172, 1396 |
| AxyI | CCTNAGG | 1 cut(s) | 1575 |
| BbsI | GAAGAC | 2 cut(s) | 248, 513 |
| Bbv12I | GWGCWC | 3 cut(s) | 237, 390, 1316 |
| BbvI | GCAGC | 4 cut(s) | 218, 680, 1424, 1685 |
| BccI | CCATC | 7 cut(s) | 63, 284, 317, 353, 951, 1487, 1532 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 370, 404 |
| BciT130I | CCWGG | 1 cut(s) | 759 |
| BclI | TGATCA | 2 cut(s) | 97, 405 |
| BcnI | CCSGG | 1 cut(s) | 1353 |
| BcoDI | GTCTC | 1 cut(s) | 655 |
| BfaI | CTAG | 5 cut(s) | 143, 624, 1037, 1113, 1158 |
| BfmI | CTRYAG | 3 cut(s) | 285, 445, 771 |
| BglII | AGATCT | 2 cut(s) | 1264, 1642 |
| BisI | GCNGC | 5 cut(s) | 207, 669, 1285, 1413, 1674 |
| BlsI | GCNGC | 5 cut(s) | 208, 670, 1286, 1414, 1675 |
| Bme1390I | CCNGG | 2 cut(s) | 759, 1353 |
| Bme18I | GGWCC | 4 cut(s) | 73, 864, 1172, 1396 |
| BmeT110I | CYCGRG | 1 cut(s) | 1072 |
| BmgT120I | GGNCC | 5 cut(s) | 73, 864, 896, 1172, 1396 |
| BmiI | GGNNCC | 2 cut(s) | 865, 866 |
| BmrFI | CCNGG | 2 cut(s) | 759, 1353 |
| BmsI | GCATC | 4 cut(s) | 461, 1399, 1460, 1685 |
| BmtI | GCTAGC | 1 cut(s) | 1161 |
| BoxI | GACNNNNGTC | 1 cut(s) | 335 |
| BpiI | GAAGAC | 2 cut(s) | 248, 513 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 62, 94, 1558, 1590 |
| BpmI | CTGGAG | 1 cut(s) | 293 |
| Bpu14I | TTCGAA | 1 cut(s) | 1419 |
| BpuEI | CTTGAG | 2 cut(s) | 1330, 1649 |
| BpuMI | CCSGG | 1 cut(s) | 1353 |
| BsaHI | GRCGYC | 1 cut(s) | 132 |
| BsaJI | CCNNGG | 2 cut(s) | 592, 1462 |
| BsaWI | WCCGGW | 1 cut(s) | 334 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 593, 953, 1100, 1452, 1576 |
| Bse118I | RCCGGY | 3 cut(s) | 334, 413, 632 |
| Bse1I | ACTGG | 1 cut(s) | 929 |
| Bse21I | CCTNAGG | 1 cut(s) | 1575 |
| BseBI | CCWGG | 1 cut(s) | 759 |
| BseDI | CCNNGG | 2 cut(s) | 592, 1462 |
| BseGI | GGATG | 4 cut(s) | 583, 943, 1475, 1676 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 593, 953, 1100, 1452, 1576 |
| BseMII | CTCAG | 3 cut(s) | 60, 741, 1589 |
| BseNI | ACTGG | 1 cut(s) | 929 |
| BseXI | GCAGC | 4 cut(s) | 218, 680, 1424, 1685 |
| BsgI | GTGCAG | 2 cut(s) | 870, 950 |
| Bsh1236I | CGCG | 2 cut(s) | 825, 1530 |
| Bsh1285I | CGRYCG | 1 cut(s) | 339 |
| BshFI | GGCC | 1 cut(s) | 898 |
| BshTI | ACCGGT | 1 cut(s) | 334 |
| BsiEI | CGRYCG | 1 cut(s) | 339 |
| BsiHKAI | GWGCWC | 3 cut(s) | 237, 390, 1316 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1072 |
| BsiSI | CCGG | 4 cut(s) | 335, 414, 633, 1352 |
| BslFI | GGGAC | 3 cut(s) | 482, 868, 877 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 593, 953, 1100, 1452, 1576 |
| BsmAI | GTCTC | 1 cut(s) | 655 |
| BsmFI | GGGAC | 3 cut(s) | 482, 868, 877 |
| BsnI | GGCC | 1 cut(s) | 898 |
| BsoBI | CYCGRG | 1 cut(s) | 1072 |
| Bsp119I | TTCGAA | 1 cut(s) | 1419 |
| Bsp1286I | GDGCHC | 3 cut(s) | 237, 390, 1316 |
| Bsp1407I | TGTACA | 1 cut(s) | 519 |
| Bsp19I | CCATGG | 1 cut(s) | 592 |
| BspACI | CCGC | 4 cut(s) | 180, 825, 888, 1284 |
| BspANI | GGCC | 1 cut(s) | 898 |
| BspCNI | CTCAG | 3 cut(s) | 61, 740, 1588 |
| BspFNI | CGCG | 2 cut(s) | 825, 1530 |
| BspHI | TCATGA | 2 cut(s) | 100, 610 |
| BspLI | GGNNCC | 2 cut(s) | 865, 866 |
| BspMAI | CTGCAG | 1 cut(s) | 289 |
| BspOI | GCTAGC | 1 cut(s) | 1161 |
| BspPI | GGATC | 2 cut(s) | 163, 1523 |
| BspT104I | TTCGAA | 1 cut(s) | 1419 |
| BsrFI | RCCGGY | 3 cut(s) | 334, 413, 632 |
| BsrGI | TGTACA | 1 cut(s) | 519 |
| BsrI | ACTGG | 1 cut(s) | 929 |
| BssAI | RCCGGY | 3 cut(s) | 334, 413, 632 |
| BssECI | CCNNGG | 2 cut(s) | 592, 1462 |
| BssNI | GRCGYC | 1 cut(s) | 132 |
| BssT1I | CCWWGG | 1 cut(s) | 592 |
| Bst2UI | CCWGG | 1 cut(s) | 759 |
| Bst4CI | ACNGT | 2 cut(s) | 1033, 1466 |
| BstACI | GRCGYC | 1 cut(s) | 132 |
| BstAUI | TGTACA | 1 cut(s) | 519 |
| BstBI | TTCGAA | 1 cut(s) | 1419 |
| BstC8I | GCNNGC | 4 cut(s) | 84, 1079, 1159, 1567 |
| BstDEI | CTNAG | 4 cut(s) | 69, 727, 884, 1575 |
| BstDSI | CCRYGG | 2 cut(s) | 592, 1462 |
| BstF5I | GGATG | 4 cut(s) | 583, 943, 1475, 1676 |
| BstFNI | CGCG | 2 cut(s) | 825, 1530 |
| BstMAI | GTCTC | 1 cut(s) | 655 |
| BstMCI | CGRYCG | 1 cut(s) | 339 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 131, 1154 |
| BstNI | CCWGG | 1 cut(s) | 759 |
| BstNSI | RCATGY | 1 cut(s) | 668 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 335 |
| BstSCI | CCNGG | 2 cut(s) | 757, 1351 |
| BstSFI | CTRYAG | 3 cut(s) | 285, 445, 771 |
| BstUI | CGCG | 2 cut(s) | 825, 1530 |
| BstV1I | GCAGC | 4 cut(s) | 218, 680, 1424, 1685 |
| BstV2I | GAAGAC | 2 cut(s) | 248, 513 |
| BstX2I | RGATCY | 2 cut(s) | 1264, 1642 |
| BstYI | RGATCY | 2 cut(s) | 1264, 1642 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1575 |
| BsuRI | GGCC | 1 cut(s) | 898 |
| BtgI | CCRYGG | 2 cut(s) | 592, 1462 |
| BtsCI | GGATG | 4 cut(s) | 583, 943, 1475, 1676 |
| BtsIMutI | CAGTG | 2 cut(s) | 135, 1196 |
| Cac8I | GCNNGC | 4 cut(s) | 84, 1079, 1159, 1567 |
| CciI | TCATGA | 2 cut(s) | 100, 610 |
| Cfr10I | RCCGGY | 3 cut(s) | 334, 413, 632 |
| Cfr13I | GGNCC | 5 cut(s) | 73, 864, 896, 1172, 1396 |
| CseI | GACGC | 2 cut(s) | 140, 1328 |
| CsiI | ACCWGGT | 1 cut(s) | 757 |
| Csp6I | GTAC | 4 cut(s) | 374, 520, 821, 1116 |
| CspAI | ACCGGT | 1 cut(s) | 334 |
| CviAII | CATG | 8 cut(s) | 101, 403, 472, 593, 611, 665, 722, 1288 |
| CviQI | GTAC | 4 cut(s) | 374, 520, 821, 1116 |
| DdeI | CTNAG | 4 cut(s) | 69, 727, 884, 1575 |
| EciI | GGCGGA | 1 cut(s) | 195 |
| Eco130I | CCWWGG | 1 cut(s) | 592 |
| Eco47I | GGWCC | 4 cut(s) | 73, 864, 1172, 1396 |
| Eco57I | CTGAAG | 1 cut(s) | 1623 |
| Eco81I | CCTNAGG | 1 cut(s) | 1575 |
| Eco88I | CYCGRG | 1 cut(s) | 1072 |
| EcoO109I | RGGNCCY | 2 cut(s) | 864, 1172 |
| EcoRII | CCWGG | 1 cut(s) | 757 |
| EcoT14I | CCWWGG | 1 cut(s) | 592 |
| ErhI | CCWWGG | 1 cut(s) | 592 |
| FaeI | CATG | 8 cut(s) | 104, 406, 475, 596, 614, 668, 725, 1291 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 728, 760, 1621, 1653 |
| FaqI | GGGAC | 3 cut(s) | 482, 868, 877 |
| FatI | CATG | 8 cut(s) | 100, 402, 471, 592, 610, 664, 721, 1287 |
| FauNDI | CATATG | 2 cut(s) | 160, 493 |
| FbaI | TGATCA | 2 cut(s) | 97, 405 |
| FblI | GTMKAC | 1 cut(s) | 420 |
| Fnu4HI | GCNGC | 5 cut(s) | 207, 669, 1285, 1413, 1674 |
| FokI | GGATG | 4 cut(s) | 570, 930, 1482, 1663 |
| Fsp4HI | GCNGC | 5 cut(s) | 207, 669, 1285, 1413, 1674 |
| FspBI | CTAG | 5 cut(s) | 143, 624, 1037, 1113, 1158 |
| GluI | GCNGC | 5 cut(s) | 207, 669, 1285, 1413, 1674 |
| GsuI | CTGGAG | 1 cut(s) | 293 |
| HaeIII | GGCC | 1 cut(s) | 898 |
| HapII | CCGG | 4 cut(s) | 335, 414, 633, 1352 |
| HgaI | GACGC | 2 cut(s) | 140, 1328 |
| Hin1I | GRCGYC | 1 cut(s) | 132 |
| Hin1II | CATG | 8 cut(s) | 104, 406, 475, 596, 614, 668, 725, 1291 |
| HinfI | GANTC | 7 cut(s) | 777, 791, 902, 1221, 1380, 1436, 1658 |
| HpaII | CCGG | 4 cut(s) | 335, 414, 633, 1352 |
| HphI | GGTGA | 3 cut(s) | 800, 1313, 1508 |
| Hpy166II | GTNNAC | 4 cut(s) | 76, 421, 520, 1304 |
| Hpy8I | GTNNAC | 4 cut(s) | 76, 421, 520, 1304 |
| Hpy99I | CGWCG | 2 cut(s) | 134, 956 |
| HpyAV | CCTTC | 7 cut(s) | 146, 574, 611, 1108, 1355, 1426, 1477 |
| HpyCH4III | ACNGT | 2 cut(s) | 1033, 1466 |
| HpyCH4IV | ACGT | 3 cut(s) | 168, 276, 951 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 131, 1154 |
| HpyF3I | CTNAG | 4 cut(s) | 69, 727, 884, 1575 |
| HpySE526I | ACGT | 3 cut(s) | 168, 276, 951 |
| Hsp92I | GRCGYC | 1 cut(s) | 132 |
| Hsp92II | CATG | 8 cut(s) | 104, 406, 475, 596, 614, 668, 725, 1291 |
| KflI | GGGWCCC | 1 cut(s) | 864 |
| Ksp22I | TGATCA | 2 cut(s) | 97, 405 |
| Lsp1109I | GCAGC | 4 cut(s) | 218, 680, 1424, 1685 |
| LweI | GCATC | 4 cut(s) | 461, 1399, 1460, 1685 |
| MabI | ACCWGGT | 1 cut(s) | 757 |
| MaeI | CTAG | 5 cut(s) | 143, 624, 1037, 1113, 1158 |
| MaeII | ACGT | 3 cut(s) | 168, 276, 951 |
| MaeIII | GTNAC | 3 cut(s) | 550, 1027, 1507 |
| MfeI | CAATTG | 3 cut(s) | 858, 1011, 1023 |
| MflI | RGATCY | 2 cut(s) | 1264, 1642 |
| MhlI | GDGCHC | 3 cut(s) | 237, 390, 1316 |
| MluCI | AATT | 9 cut(s) | 627, 858, 982, 1011, 1023, 1135, 1238, 1243, 1617 |
| MluI | ACGCGT | 1 cut(s) | 1528 |
| MlyI | GAGTC | 3 cut(s) | 786, 911, 1230 |
| MmeI | TCCRAC | 5 cut(s) | 129, 284, 694, 934, 1283 |
| MseI | TTAA | 6 cut(s) | 483, 684, 990, 1325, 1368, 1690 |
| MslI | CAYNNNNRTG | 4 cut(s) | 193, 401, 496, 515 |
| MspA1I | CMGCKG | 1 cut(s) | 671 |
| MspI | CCGG | 4 cut(s) | 335, 414, 633, 1352 |
| MspR9I | CCNGG | 2 cut(s) | 759, 1353 |
| MunI | CAATTG | 3 cut(s) | 858, 1011, 1023 |
| MvaI | CCWGG | 1 cut(s) | 759 |
| MvnI | CGCG | 2 cut(s) | 825, 1530 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 131, 1154 |
| NciI | CCSGG | 1 cut(s) | 1353 |
| NcoI | CCATGG | 1 cut(s) | 592 |
| NdeI | CATATG | 2 cut(s) | 160, 493 |
| NheI | GCTAGC | 1 cut(s) | 1157 |
| NlaIII | CATG | 8 cut(s) | 104, 406, 475, 596, 614, 668, 725, 1291 |
| NlaIV | GGNNCC | 2 cut(s) | 865, 866 |
| NmeAIII | GCCGAG | 1 cut(s) | 1457 |
| NspI | RCATGY | 1 cut(s) | 668 |
| NspV | TTCGAA | 1 cut(s) | 1419 |
| OliI | CACNNNNGTG | 1 cut(s) | 515 |
| PaeR7I | CTCGAG | 1 cut(s) | 1072 |
| PagI | TCATGA | 2 cut(s) | 100, 610 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 86, 345, 1527 |
| PfeI | GAWTC | 4 cut(s) | 791, 1380, 1436, 1658 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 593 |
| PinAI | ACCGGT | 1 cut(s) | 334 |
| PkrI | GCNGC | 5 cut(s) | 208, 670, 1286, 1414, 1675 |
| PleI | GAGTC | 3 cut(s) | 785, 910, 1229 |
| PpsI | GAGTC | 3 cut(s) | 785, 910, 1229 |
| PpuMI | RGGWCCY | 2 cut(s) | 864, 1172 |
| PshAI | GACNNNNGTC | 1 cut(s) | 335 |
| Psp5II | RGGWCCY | 2 cut(s) | 864, 1172 |
| Psp6I | CCWGG | 1 cut(s) | 757 |
| PspGI | CCWGG | 1 cut(s) | 757 |
| PspN4I | GGNNCC | 2 cut(s) | 865, 866 |
| PspPI | GGNCC | 5 cut(s) | 73, 864, 896, 1172, 1396 |
| PspPPI | RGGWCCY | 2 cut(s) | 864, 1172 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 613, 645 |
| PstI | CTGCAG | 1 cut(s) | 289 |
| PsuI | RGATCY | 2 cut(s) | 1264, 1642 |
| PvuII | CAGCTG | 1 cut(s) | 671 |
| RsaI | GTAC | 4 cut(s) | 375, 521, 822, 1117 |
| RsaNI | GTAC | 4 cut(s) | 374, 520, 821, 1116 |
| RseI | CAYNNNNRTG | 4 cut(s) | 193, 401, 496, 515 |
| SaqAI | TTAA | 6 cut(s) | 483, 684, 990, 1325, 1368, 1690 |
| SatI | GCNGC | 5 cut(s) | 207, 669, 1285, 1413, 1674 |
| Sau96I | GGNCC | 5 cut(s) | 73, 864, 896, 1172, 1396 |
| SchI | GAGTC | 3 cut(s) | 786, 911, 1230 |
| ScrFI | CCNGG | 2 cut(s) | 759, 1353 |
| SduI | GDGCHC | 3 cut(s) | 237, 390, 1316 |
| SexAI | ACCWGGT | 1 cut(s) | 757 |
| SfaNI | GCATC | 4 cut(s) | 461, 1399, 1460, 1685 |
| SfcI | CTRYAG | 3 cut(s) | 285, 445, 771 |
| Sfr274I | CTCGAG | 1 cut(s) | 1072 |
| SfuI | TTCGAA | 1 cut(s) | 1419 |
| SinI | GGWCC | 4 cut(s) | 73, 864, 1172, 1396 |
| SlaI | CTCGAG | 1 cut(s) | 1072 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 193, 401, 496, 515 |
| SmlI | CTYRAG | 3 cut(s) | 1072, 1309, 1664 |
| SmoI | CTYRAG | 3 cut(s) | 1072, 1309, 1664 |
| Sse9I | AATT | 9 cut(s) | 627, 858, 982, 1011, 1023, 1135, 1238, 1243, 1617 |
| SsiI | CCGC | 4 cut(s) | 180, 825, 888, 1284 |
| SspI | AATATT | 3 cut(s) | 65, 925, 1372 |
| SspMI | CTAG | 5 cut(s) | 143, 624, 1037, 1113, 1158 |
| StyD4I | CCNGG | 2 cut(s) | 757, 1351 |
| StyI | CCWWGG | 1 cut(s) | 592 |
| TaaI | ACNGT | 2 cut(s) | 1033, 1466 |
| TaiI | ACGT | 3 cut(s) | 171, 279, 954 |
| TaqI | TCGA | 5 cut(s) | 243, 339, 1073, 1139, 1419 |
| TasI | AATT | 9 cut(s) | 627, 858, 982, 1011, 1023, 1135, 1238, 1243, 1617 |
| TatI | WGTACW | 2 cut(s) | 519, 1115 |
| TauI | GCSGC | 1 cut(s) | 1287 |
| TfiI | GAWTC | 4 cut(s) | 791, 1380, 1436, 1658 |
| Tru1I | TTAA | 6 cut(s) | 483, 684, 990, 1325, 1368, 1690 |
| Tru9I | TTAA | 6 cut(s) | 483, 684, 990, 1325, 1368, 1690 |
| TscAI | CASTG | 2 cut(s) | 142, 1203 |
| TseI | GCWGC | 4 cut(s) | 206, 668, 1412, 1673 |
| TspDTI | ATGAA | 6 cut(s) | 572, 627, 690, 710, 976, 1304 |
| TspGWI | ACGGA | 2 cut(s) | 813, 1493 |
| TspRI | CASTG | 2 cut(s) | 142, 1203 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 593 |
| VpaK11BI | GGWCC | 4 cut(s) | 73, 864, 1172, 1396 |
| XapI | RAATTY | 3 cut(s) | 1135, 1238, 1617 |
| XbaI | TCTAGA | 1 cut(s) | 142 |
| XceI | RCATGY | 1 cut(s) | 668 |
| XhoI | CTCGAG | 1 cut(s) | 1072 |
| XmiI | GTMKAC | 1 cut(s) | 420 |
| XspI | CTAG | 5 cut(s) | 143, 624, 1037, 1113, 1158 |