FvH4_6g09340

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000533
Physical Location & Seq
Forward (+)
5555101 .. 5555567
467 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g09340.t1

Sequence Viewer

Length: 354 bp
ATGAAAAAGACTGGAGAGTTGTTAAACACCAAGGCACTGCAAGCATTTAAGGTTGAAGCTTCGTCGAGCACAAAAGCTTACAAGTTTTCTGGTCCAACTTTATCCGTGAACCAGACAAACGATGCATCTTTCGGAGAATTTTGCCGTGATACAGTATCAACAATTTGGCATTACCACGGCGGATGCCTCGTCGGAAAGGTGGTCGATAGCAGCCTGCGGGTCATGGGAATCGATACGTTACGTGTTGTTGACGCATCCACATTCAATTTCACACCGGGGACCAATCGTCAGGCCACCCTTATGATGCTGGGCAGGTACATTGGTATTAAGATGTTGCAACAAAGATCTTCATAA

Protein Analysis

118

Amino Acids

12.85

Weight (kDa)

9.64

Isoelectric Point (pI)

26.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_C PF05199 32 - 104 5.2e-17 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 303
AciI CCGC 2 cut(s) 180, 217
AcsI RAATTY 1 cut(s) 137
AfaI GTAC 1 cut(s) 317
AflIII ACRYGT 1 cut(s) 241
AgsI TTSAA 2 cut(s) 56, 265
AhdI GACNNNNNGTC 1 cut(s) 285
AluBI AGCT 2 cut(s) 59, 77
AluI AGCT 2 cut(s) 59, 77
Alw21I GWGCWC 1 cut(s) 71
AoxI GGCC 1 cut(s) 291
ApeKI GCWGC 1 cut(s) 210
ApoI RAATTY 1 cut(s) 137
AspS9I GGNCC 2 cut(s) 92, 279
AsuC2I CCSGG 1 cut(s) 276
AvaII GGWCC 2 cut(s) 92, 279
BaeI ACNNNNGTAYC 2 cut(s) 307, 340
Bbv12I GWGCWC 1 cut(s) 71
BbvI GCAGC 1 cut(s) 222
BceAI ACGGC 2 cut(s) 129, 193
BcnI CCSGG 1 cut(s) 276
BfuAI ACCTGC 1 cut(s) 303
BglII AGATCT 1 cut(s) 344
BisI GCNGC 1 cut(s) 211
BlsI GCNGC 1 cut(s) 212
Bme1390I CCNGG 1 cut(s) 276
Bme18I GGWCC 2 cut(s) 92, 279
BmeRI GACNNNNNGTC 1 cut(s) 285
BmgT120I GGNCC 2 cut(s) 92, 279
BmiI GGNNCC 1 cut(s) 280
BmrFI CCNGG 1 cut(s) 276
BmsI GCATC 5 cut(s) 112, 134, 173, 263, 294
BpmI CTGGAG 1 cut(s) 33
BpuMI CCSGG 1 cut(s) 276
Bsa29I ATCGAT 1 cut(s) 231
BsaAI YACGTR 1 cut(s) 242
BsaJI CCNNGG 3 cut(s) 30, 175, 275
Bse1I ACTGG 1 cut(s) 16
BseCI ATCGAT 1 cut(s) 231
BseDI CCNNGG 3 cut(s) 30, 175, 275
BseGI GGATG 2 cut(s) 188, 254
BseNI ACTGG 1 cut(s) 16
BseXI GCAGC 1 cut(s) 222
BseYI CCCAGC 1 cut(s) 307
BshFI GGCC 1 cut(s) 293
BshVI ATCGAT 1 cut(s) 231
BsiHKAI GWGCWC 1 cut(s) 71
BsiSI CCGG 1 cut(s) 275
BslFI GGGAC 1 cut(s) 292
BsmFI GGGAC 1 cut(s) 292
BsnI GGCC 1 cut(s) 293
Bsp1286I GDGCHC 1 cut(s) 71
Bsp143I GATC 1 cut(s) 344
BspACI CCGC 2 cut(s) 180, 217
BspANI GGCC 1 cut(s) 293
BspDI ATCGAT 1 cut(s) 231
BspLI GGNNCC 1 cut(s) 280
BspMI ACCTGC 1 cut(s) 303
BsrI ACTGG 1 cut(s) 16
BssECI CCNNGG 3 cut(s) 30, 175, 275
BssMI GATC 1 cut(s) 344
BssT1I CCWWGG 1 cut(s) 30
Bst4CI ACNGT 1 cut(s) 154
BstBAI YACGTR 1 cut(s) 242
BstC8I GCNNGC 2 cut(s) 42, 215
BstDSI CCRYGG 1 cut(s) 175
BstF5I GGATG 2 cut(s) 188, 254
BstKTI GATC 1 cut(s) 347
BstMBI GATC 1 cut(s) 344
BstMWI GCNNNNNNNGC 1 cut(s) 41
BstSCI CCNGG 1 cut(s) 274
BstV1I GCAGC 1 cut(s) 222
BstX2I RGATCY 1 cut(s) 344
BstYI RGATCY 1 cut(s) 344
Bsu15I ATCGAT 1 cut(s) 231
BsuRI GGCC 1 cut(s) 293
BsuTUI ATCGAT 1 cut(s) 231
BtgI CCRYGG 1 cut(s) 175
BtsCI GGATG 2 cut(s) 188, 254
BtsI GCAGTG 1 cut(s) 35
BtsIMutI CAGTG 1 cut(s) 35
BveI ACCTGC 1 cut(s) 303
Cac8I GCNNGC 2 cut(s) 42, 215
Cfr13I GGNCC 2 cut(s) 92, 279
ClaI ATCGAT 1 cut(s) 231
CseI GACGC 1 cut(s) 260
Csp6I GTAC 1 cut(s) 316
CviAII CATG 1 cut(s) 223
CviJI RGCY 4 cut(s) 59, 77, 213, 293
CviKI_1 RGCY 4 cut(s) 59, 77, 213, 293
CviQI GTAC 1 cut(s) 316
DpnI GATC 1 cut(s) 346
DpnII GATC 1 cut(s) 344
DriI GACNNNNNGTC 1 cut(s) 285
Eam1105I GACNNNNNGTC 1 cut(s) 285
EciI GGCGGA 1 cut(s) 195
Eco130I CCWWGG 1 cut(s) 30
Eco47I GGWCC 2 cut(s) 92, 279
EcoT14I CCWWGG 1 cut(s) 30
EcoT22I ATGCAT 1 cut(s) 127
ErhI CCWWGG 1 cut(s) 30
FaeI CATG 1 cut(s) 226
FaiI YATR 3 cut(s) 224, 302, 352
FaqI GGGAC 1 cut(s) 292
FatI CATG 1 cut(s) 222
FauI CCCGC 1 cut(s) 210
Fnu4HI GCNGC 1 cut(s) 211
FokI GGATG 2 cut(s) 195, 241
Fsp4HI GCNGC 1 cut(s) 211
GluI GCNGC 1 cut(s) 211
GsaI CCCAGC 1 cut(s) 311
GsuI CTGGAG 1 cut(s) 33
HaeIII GGCC 1 cut(s) 293
HapII CCGG 1 cut(s) 275
HgaI GACGC 1 cut(s) 260
Hin1II CATG 1 cut(s) 226
HincII GTYRAC 1 cut(s) 250
HindII GTYRAC 1 cut(s) 250
HindIII AAGCTT 2 cut(s) 57, 75
HinfI GANTC 1 cut(s) 228
HpaII CCGG 1 cut(s) 275
Hpy166II GTNNAC 2 cut(s) 109, 250
Hpy188I TCNGA 2 cut(s) 134, 194
Hpy8I GTNNAC 2 cut(s) 109, 250
Hpy99I CGWCG 2 cut(s) 67, 194
HpyCH4III ACNGT 1 cut(s) 154
HpyCH4IV ACGT 2 cut(s) 236, 241
HpyCH4V TGCA 3 cut(s) 40, 125, 337
HpyF10VI GCNNNNNNNGC 1 cut(s) 41
HpySE526I ACGT 2 cut(s) 236, 241
Hsp92II CATG 1 cut(s) 226
Kzo9I GATC 1 cut(s) 344
LpnPI CCDG 7 cut(s) 75, 125, 227, 275, 288, 293, 298
Lsp1109I GCAGC 1 cut(s) 222
LweI GCATC 5 cut(s) 112, 134, 173, 263, 294
MaeII ACGT 2 cut(s) 236, 241
MaeIII GTNAC 1 cut(s) 237
MalI GATC 1 cut(s) 346
MboI GATC 1 cut(s) 344
MboII GAAGA 1 cut(s) 339
MflI RGATCY 1 cut(s) 344
MhlI GDGCHC 1 cut(s) 71
MluCI AATT 3 cut(s) 137, 162, 265
MmeI TCCRAC 2 cut(s) 119, 172
MnlI CCTC 1 cut(s) 197
Mph1103I ATGCAT 1 cut(s) 127
MseI TTAA 3 cut(s) 23, 48, 327
MslI CAYNNNNRTG 1 cut(s) 299
MspI CCGG 1 cut(s) 275
MspR9I CCNGG 1 cut(s) 276
MwoI GCNNNNNNNGC 1 cut(s) 41
NciI CCSGG 1 cut(s) 276
NdeII GATC 1 cut(s) 344
NlaIII CATG 1 cut(s) 226
NlaIV GGNNCC 1 cut(s) 280
NsiI ATGCAT 1 cut(s) 127
PfeI GAWTC 1 cut(s) 228
PkrI GCNGC 1 cut(s) 212
Ppu21I YACGTR 1 cut(s) 242
PspFI CCCAGC 1 cut(s) 307
PspN4I GGNNCC 1 cut(s) 280
PspPI GGNCC 2 cut(s) 92, 279
PsuI RGATCY 1 cut(s) 344
RsaI GTAC 1 cut(s) 317
RsaNI GTAC 1 cut(s) 316
RseI CAYNNNNRTG 1 cut(s) 299
SaqAI TTAA 3 cut(s) 23, 48, 327
SatI GCNGC 1 cut(s) 211
Sau3AI GATC 1 cut(s) 344
Sau96I GGNCC 2 cut(s) 92, 279
ScrFI CCNGG 1 cut(s) 276
SduI GDGCHC 1 cut(s) 71
SetI ASST 7 cut(s) 54, 61, 79, 201, 239, 244, 317
SfaNI GCATC 5 cut(s) 112, 134, 173, 263, 294
SinI GGWCC 2 cut(s) 92, 279
SmiMI CAYNNNNRTG 1 cut(s) 299
Sse9I AATT 3 cut(s) 137, 162, 265
SsiI CCGC 2 cut(s) 180, 217
StyD4I CCNGG 1 cut(s) 274
StyI CCWWGG 1 cut(s) 30
TaaI ACNGT 1 cut(s) 154
TaiI ACGT 2 cut(s) 239, 244
TaqI TCGA 3 cut(s) 65, 204, 231
TasI AATT 3 cut(s) 137, 162, 265
TfiI GAWTC 1 cut(s) 228
Tru1I TTAA 3 cut(s) 23, 48, 327
Tru9I TTAA 3 cut(s) 23, 48, 327
TscAI CASTG 1 cut(s) 42
TseI GCWGC 1 cut(s) 210
TspDTI ATGAA 2 cut(s) 17, 339
TspGWI ACGGA 1 cut(s) 94
TspRI CASTG 1 cut(s) 42
VpaK11BI GGWCC 2 cut(s) 92, 279
XapI RAATTY 1 cut(s) 137
Zsp2I ATGCAT 1 cut(s) 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.