Rh4CG129200

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000533
Physical Location & Seq
Reverse (-)
25321560 .. 25323659
2100 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG129200.1

Sequence Viewer

Length: 1170 bp
ATGGACAAATCAACAATAGGTGCTAGACTACTGCTCTTGAACCTTTTTGTACTTGGCTTTCTTGGTCAATCAACAGTTCATTCATTGGCCAATGCATCTACACCTAATCATGATTTTAGCTACATGAAGATTGTGAAGGATGCCACTGATCTACCGTTGGAAGATAAATATGACTATATTGTAGTTGGGGGAGGAACAGCAGGTTGTCCATTAGCAGCAACATTATCAGCAAGCTACTCGGTGCTTGTTCTTGAAAGGGGTAGTGCTCCTACTGCACATCCAAGTGTCTTGCGAGAAGAAGGGTTTCTGAGTAATCTCATGCAAGAAGATGATGGTAAGATCACACCAGCTCAAAAGTTTAAGTCTGAAGACGGTGTTGCTATTGTCAGAGGAAGGGTTCTTGGTGGCTCAAGCATGATCAATGCTGGTTTCTACTCCAAAGCTGACAGGGAATTCTTTGTTAAATCTGGAATTAAATGGGACATGGATTTGGTTGATAAGGCATATCACTGGGTTGAAAGCACTGTCGTCTCTCGTCCAAATCTGTCACGTTCAAATTGGCAATCTATTGTGAAAGAAGCATTGTTGGAGGCTGGTGTTGGTCCAAACAATGGATTCAGTTTGGATCACATTAAGGGAACTAAAGTTGGCGGTTCTACTTTTGATACACATGGAAGGAGATACGGAGCTGTTGAATTGCTTAATAGAGGAAACCTAAAGAACTTGCGAGTGGGAATTCATGCCACAGTCGAGAAAATCATCTTCTCTTCCAAAGAATCAGGTGTTTCGGCTCGAGGGGTGGTATATATTGATTCTAATGGGAGGTCTCATCGGGCATTTGTACGTGGTAAGGGAGAGGTTATTCTGAGTGCAGGGGCAATTGGGAGTCCTCAGCTTCTACTTCTTAGCGGTGTTGGTCCTCAATCCTATCTATCATCTTTGAAAATCCCAGTTGTTCACCCCCAACCTCACACTGGAAATTTTATGTATGACAATCCTCGTAATTTCATTAACATTTTGTCCCCGTTTCCACTACAGCCCTCAACTGTAAAGATTGTCGGCATTACCGACCATTTCTACATAGAGACCCTCTCTAGCATGCCCATTTCTACTACACCCTACAGTGTTTTTCCAGATCCGGCTAAACTTACAAAGATAAATTCAAGTTAG

Protein Analysis

389

Amino Acids

42.04

Weight (kDa)

9.06

Isoelectric Point (pI)

34.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_N PF00732 57 - 310 1.1e-21 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 191
AccB7I CCANNNNNTGG 1 cut(s) 611
AciI CCGC 2 cut(s) 651, 909
AclWI GGATC 2 cut(s) 633, 1130
AcoI YGGCCR 1 cut(s) 87
AcsI RAATTY 4 cut(s) 452, 735, 979, 1159
AcuI CTGAAG 1 cut(s) 387
AfaI GTAC 2 cut(s) 51, 843
AfiI CCNNNNNNNGG 2 cut(s) 611, 974
AgsI TTSAA 7 cut(s) 40, 254, 518, 555, 695, 943, 1164
AluBI AGCT 6 cut(s) 120, 234, 350, 443, 689, 895
AluI AGCT 6 cut(s) 120, 234, 350, 443, 689, 895
Alw21I GWGCWC 1 cut(s) 268
Alw26I GTCTC 3 cut(s) 535, 831, 1079
AlwI GGATC 2 cut(s) 633, 1130
Ama87I CYCGRG 1 cut(s) 792
AoxI GGCC 1 cut(s) 87
ApeKI GCWGC 1 cut(s) 215
ApoI RAATTY 4 cut(s) 452, 735, 979, 1159
Asp700I GAANNNNTTC 1 cut(s) 303
AspS9I GGNCC 2 cut(s) 602, 917
AsuHPI GGTGA 1 cut(s) 950
AvaI CYCGRG 1 cut(s) 792
AvaII GGWCC 2 cut(s) 602, 917
BalI TGGCCA 1 cut(s) 89
BbsI GAAGAC 1 cut(s) 375
Bbv12I GWGCWC 1 cut(s) 268
BbvCI CCTCAGC 1 cut(s) 891
BbvI GCAGC 1 cut(s) 227
BccI CCATC 1 cut(s) 326
BcgI CGANNNNNNTGC 2 cut(s) 219, 253
BclI TGATCA 1 cut(s) 417
BcoDI GTCTC 3 cut(s) 535, 831, 1079
BfaI CTAG 2 cut(s) 24, 1095
BfmI CTRYAG 2 cut(s) 1034, 1120
BfuAI ACCTGC 1 cut(s) 191
BisI GCNGC 1 cut(s) 216
BlsI GCNGC 1 cut(s) 217
Bme18I GGWCC 2 cut(s) 602, 917
BmeT110I CYCGRG 1 cut(s) 792
BmgT120I GGNCC 2 cut(s) 602, 917
BmrI ACTGGG 2 cut(s) 520, 944
BmsI GCATC 2 cut(s) 104, 130
BmuI ACTGGG 2 cut(s) 520, 944
BpiI GAAGAC 1 cut(s) 375
BplI GAGNNNNNCTC 2 cut(s) 1076, 1108
Bpu10I CCTNAGC 1 cut(s) 891
BpuEI CTTGAG 1 cut(s) 394
BsaAI YACGTR 1 cut(s) 845
BsaI GGTCTC 2 cut(s) 831, 1079
Bsc4I CCNNNNNNNGG 2 cut(s) 611, 974
Bse1I ACTGG 3 cut(s) 515, 950, 979
BseGI GGATG 2 cut(s) 145, 277
BseLI CCNNNNNNNGG 2 cut(s) 611, 974
BseMII CTCAG 3 cut(s) 299, 857, 905
BseNI ACTGG 3 cut(s) 515, 950, 979
BseXI GCAGC 1 cut(s) 227
BsgI GTGCAG 2 cut(s) 258, 891
BshFI GGCC 1 cut(s) 89
BsiHKAI GWGCWC 1 cut(s) 268
BsiHKCI CYCGRG 1 cut(s) 792
BsiSI CCGG 1 cut(s) 1139
BslFI GGGAC 2 cut(s) 494, 1006
BslI CCNNNNNNNGG 2 cut(s) 611, 974
BsmAI GTCTC 3 cut(s) 535, 831, 1079
BsmBI CGTCTC 1 cut(s) 535
BsmFI GGGAC 2 cut(s) 494, 1006
BsnI GGCC 1 cut(s) 89
Bso31I GGTCTC 2 cut(s) 831, 1079
BsoBI CYCGRG 1 cut(s) 792
Bsp1286I GDGCHC 1 cut(s) 268
Bsp143I GATC 5 cut(s) 148, 339, 417, 625, 1135
BspACI CCGC 2 cut(s) 651, 909
BspANI GGCC 1 cut(s) 89
BspCNI CTCAG 3 cut(s) 300, 858, 904
BspHI TCATGA 1 cut(s) 109
BspMI ACCTGC 1 cut(s) 191
BspPI GGATC 2 cut(s) 633, 1130
BspTNI GGTCTC 2 cut(s) 831, 1079
BsrI ACTGG 3 cut(s) 515, 950, 979
BssMI GATC 5 cut(s) 148, 339, 417, 625, 1135
Bst4CI ACNGT 7 cut(s) 76, 156, 374, 526, 748, 1048, 1124
Bst6I CTCTTC 1 cut(s) 772
BstBAI YACGTR 1 cut(s) 845
BstC8I GCNNGC 2 cut(s) 232, 1100
BstDEI CTNAG 4 cut(s) 308, 866, 891, 905
BstF5I GGATG 2 cut(s) 145, 277
BstKTI GATC 5 cut(s) 151, 342, 420, 628, 1138
BstMAI GTCTC 3 cut(s) 535, 831, 1079
BstMBI GATC 5 cut(s) 148, 339, 417, 625, 1135
BstMWI GCNNNNNNNGC 1 cut(s) 272
BstNSI RCATGY 1 cut(s) 1102
BstSFI CTRYAG 2 cut(s) 1034, 1120
BstV1I GCAGC 1 cut(s) 227
BstV2I GAAGAC 1 cut(s) 375
BstX2I RGATCY 1 cut(s) 1135
BstYI RGATCY 1 cut(s) 1135
BsuRI GGCC 1 cut(s) 89
BtsCI GGATG 2 cut(s) 145, 277
BtsIMutI CAGTG 5 cut(s) 144, 508, 522, 972, 1129
BveI ACCTGC 1 cut(s) 191
Cac8I GCNNGC 2 cut(s) 232, 1100
CciI TCATGA 1 cut(s) 109
Cfr13I GGNCC 2 cut(s) 602, 917
Csp6I GTAC 2 cut(s) 50, 842
CviAII CATG 8 cut(s) 110, 124, 319, 415, 484, 671, 740, 1099
CviQI GTAC 2 cut(s) 50, 842
DdeI CTNAG 4 cut(s) 308, 866, 891, 905
DpnI GATC 5 cut(s) 150, 341, 419, 627, 1137
DpnII GATC 5 cut(s) 148, 339, 417, 625, 1135
EaeI YGGCCR 1 cut(s) 87
Eam1104I CTCTTC 1 cut(s) 772
EarI CTCTTC 1 cut(s) 772
Eco31I GGTCTC 2 cut(s) 831, 1079
Eco47I GGWCC 2 cut(s) 602, 917
Eco57I CTGAAG 1 cut(s) 387
Eco88I CYCGRG 1 cut(s) 792
EcoRI GAATTC 2 cut(s) 452, 735
EcoT22I ATGCAT 1 cut(s) 97
Esp3I CGTCTC 1 cut(s) 535
FaeI CATG 8 cut(s) 113, 127, 322, 418, 487, 674, 743, 1102
FaqI GGGAC 2 cut(s) 494, 1006
FatI CATG 8 cut(s) 109, 123, 318, 414, 483, 670, 739, 1098
FbaI TGATCA 1 cut(s) 417
Fnu4HI GCNGC 1 cut(s) 216
FokI GGATG 2 cut(s) 152, 264
Fsp4HI GCNGC 1 cut(s) 216
FspBI CTAG 2 cut(s) 24, 1095
GluI GCNGC 1 cut(s) 216
HaeIII GGCC 1 cut(s) 89
HapII CCGG 1 cut(s) 1139
Hin1II CATG 8 cut(s) 113, 127, 322, 418, 487, 674, 743, 1102
HinfI GANTC 4 cut(s) 615, 776, 812, 886
HpaII CCGG 1 cut(s) 1139
HphI GGTGA 1 cut(s) 950
Hpy166II GTNNAC 1 cut(s) 958
Hpy188I TCNGA 4 cut(s) 309, 367, 389, 867
Hpy188III TCNNGA 6 cut(s) 37, 110, 251, 468, 751, 1133
Hpy8I GTNNAC 1 cut(s) 958
HpyAV CCTTC 4 cut(s) 130, 293, 387, 669
HpyCH4III ACNGT 7 cut(s) 76, 156, 374, 526, 748, 1048, 1124
HpyCH4IV ACGT 2 cut(s) 550, 844
HpyCH4V TGCA 4 cut(s) 95, 275, 322, 872
HpyF10VI GCNNNNNNNGC 1 cut(s) 272
HpyF3I CTNAG 4 cut(s) 308, 866, 891, 905
HpySE526I ACGT 2 cut(s) 550, 844
Hsp92II CATG 8 cut(s) 113, 127, 322, 418, 487, 674, 743, 1102
Ksp22I TGATCA 1 cut(s) 417
Kzo9I GATC 5 cut(s) 148, 339, 417, 625, 1135
LmnI GCTCC 2 cut(s) 271, 686
Lsp1109I GCAGC 1 cut(s) 227
LweI GCATC 2 cut(s) 104, 130
MaeI CTAG 2 cut(s) 24, 1095
MaeII ACGT 2 cut(s) 550, 844
MaeIII GTNAC 1 cut(s) 546
MalI GATC 5 cut(s) 150, 341, 419, 627, 1137
MboI GATC 5 cut(s) 148, 339, 417, 625, 1135
MboII GAAGA 7 cut(s) 139, 173, 308, 338, 380, 754, 759
MfeI CAATTG 1 cut(s) 879
MflI RGATCY 1 cut(s) 1135
MhlI GDGCHC 1 cut(s) 268
MlsI TGGCCA 1 cut(s) 89
MluCI AATT 9 cut(s) 452, 471, 556, 695, 735, 879, 979, 1003, 1159
MluNI TGGCCA 1 cut(s) 89
MlyI GAGTC 1 cut(s) 895
MmeI TCCRAC 2 cut(s) 138, 567
Mox20I TGGCCA 1 cut(s) 89
Mph1103I ATGCAT 1 cut(s) 97
MroXI GAANNNNTTC 1 cut(s) 303
MscI TGGCCA 1 cut(s) 89
MseI TTAA 6 cut(s) 360, 462, 474, 633, 702, 1011
MslI CAYNNNNRTG 1 cut(s) 282
Msp20I TGGCCA 1 cut(s) 89
MspI CCGG 1 cut(s) 1139
MunI CAATTG 1 cut(s) 879
MwoI GCNNNNNNNGC 1 cut(s) 272
NdeII GATC 5 cut(s) 148, 339, 417, 625, 1135
NlaIII CATG 8 cut(s) 113, 127, 322, 418, 487, 674, 743, 1102
NmuCI GTSAC 1 cut(s) 546
NsiI ATGCAT 1 cut(s) 97
NspI RCATGY 1 cut(s) 1102
PaeI GCATGC 1 cut(s) 1102
PaeR7I CTCGAG 1 cut(s) 792
PagI TCATGA 1 cut(s) 109
PcsI WCGNNNNNNNCGW 1 cut(s) 1065
PdmI GAANNNNTTC 1 cut(s) 303
PfeI GAWTC 3 cut(s) 615, 776, 812
PflMI CCANNNNNTGG 1 cut(s) 611
PkrI GCNGC 1 cut(s) 217
PleI GAGTC 1 cut(s) 894
PpsI GAGTC 1 cut(s) 894
Ppu21I YACGTR 1 cut(s) 845
PspPI GGNCC 2 cut(s) 602, 917
PspXI VCTCGAGB 1 cut(s) 792
PsuI RGATCY 1 cut(s) 1135
RsaI GTAC 2 cut(s) 51, 843
RsaNI GTAC 2 cut(s) 50, 842
RseI CAYNNNNRTG 1 cut(s) 282
SaqAI TTAA 6 cut(s) 360, 462, 474, 633, 702, 1011
SatI GCNGC 1 cut(s) 216
Sau3AI GATC 5 cut(s) 148, 339, 417, 625, 1135
Sau96I GGNCC 2 cut(s) 602, 917
SchI GAGTC 1 cut(s) 895
SduI GDGCHC 1 cut(s) 268
SfaNI GCATC 2 cut(s) 104, 130
SfcI CTRYAG 2 cut(s) 1034, 1120
Sfr274I CTCGAG 1 cut(s) 792
SinI GGWCC 2 cut(s) 602, 917
SlaI CTCGAG 1 cut(s) 792
SmiMI CAYNNNNRTG 1 cut(s) 282
SmlI CTYRAG 2 cut(s) 409, 792
SmoI CTYRAG 2 cut(s) 409, 792
SphI GCATGC 1 cut(s) 1102
Sse9I AATT 9 cut(s) 452, 471, 556, 695, 735, 879, 979, 1003, 1159
SsiI CCGC 2 cut(s) 651, 909
SspMI CTAG 2 cut(s) 24, 1095
TaaI ACNGT 7 cut(s) 76, 156, 374, 526, 748, 1048, 1124
TaiI ACGT 2 cut(s) 553, 847
TaqI TCGA 2 cut(s) 750, 793
TasI AATT 9 cut(s) 452, 471, 556, 695, 735, 879, 979, 1003, 1159
TatI WGTACW 1 cut(s) 49
TfiI GAWTC 3 cut(s) 615, 776, 812
Tru1I TTAA 6 cut(s) 360, 462, 474, 633, 702, 1011
Tru9I TTAA 6 cut(s) 360, 462, 474, 633, 702, 1011
TscAI CASTG 5 cut(s) 151, 515, 529, 979, 1129
TseFI GTSAC 1 cut(s) 546
TseI GCWGC 1 cut(s) 215
Tsp45I GTSAC 1 cut(s) 546
TspDTI ATGAA 5 cut(s) 68, 72, 140, 728, 997
TspGWI ACGGA 1 cut(s) 699
TspRI CASTG 5 cut(s) 151, 515, 529, 979, 1129
Van91I CCANNNNNTGG 1 cut(s) 611
VpaK11BI GGWCC 2 cut(s) 602, 917
XapI RAATTY 4 cut(s) 452, 735, 979, 1159
XceI RCATGY 1 cut(s) 1102
XcmI CCANNNNNNNNNTGG 1 cut(s) 971
XhoI CTCGAG 1 cut(s) 792
XmnI GAANNNNTTC 1 cut(s) 303
XspI CTAG 2 cut(s) 24, 1095
Zsp2I ATGCAT 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.