MD13G1264100.v1.1

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000533
Physical Location & Seq
Forward (+)
34020114 .. 34023648
3535 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1264100.v1.1.491

Sequence Viewer

Length: 1014 bp
ATGGAAGATAATGGCAAGACACCAGCTCAAAAGTTCACATCAGAAGAAGGTGTTCCTAATGTCAGAGGCTGGGTCTTAGATGGGTCAAGCATGATCAATGCCGGCTTCTACACCACAGCTGACAAGGAATTCTTTATGAAATCAGGGATTGAGTGGGACATGTATTCAGTTTATAAGGCATATCGGTGGGTAGAAGAAACTATTGTGTTCCCCCCAACTATGGATCGCTGGCAATCTGCTGTAGCAAAAGCATTTATGGAGGCTGATGTTGGTCCAGACAATGGACTTAATTTGGATCACATTAAAGGAACTAAAATTGGGGGTTCGACGTTTGACAACAATGGGAAAAGACATGGAGCTGTGGAGCTGCTTAATAAAGGAGATCTGAACAAATTGCGAGTTGCAGTTCAGGCCACAGTAGAGAAGATCCTCTTCTCTTCCAAGGCATCAAGTTCGTCAGCTATTGGAATCATGTATAGTGATTCGAACGGGAGGTCCCATTGTGCATATGTACGCAGGAAATGGGAGGCTGTATTGAGTGCCGGTGCCATTGGGAGCCCTCAACTTCTACAACTTAGTGGTATTGGCCCAGAATCTTATCTGTCATCTCTTAAAATCCCAGTTGTTAGTCCCCATCCATACATTGGGCAGTTTATGTATGACAATCCTTGTAACTTAATTAACATTTTGCCCCCTATTCCACTAGAGCCTTCACCTGTGAAGATTGTAGGTATTACAAGCGATTTCTACATAGAGAGTCTCTCCACTTCTCCATTTTCTACTCCACCATTTAGCCTTTTTCTAGGGGGATCCATTGTTGGAAAGGTTGTGGATAGCGGTTTGCGTGTTTTGGGGATCAATGCATTGCGTGTTATGGATGCATCCACATTCAATTTCCCACCGGGGGCGAATCCTCAGGACACCCTTATGGATCCATTGTTGGCAGGTATGTTGGCCTTAAGATGCTGCAAGAAAGATCAGCCTGAAAGGAAAAGCTATTCAAAAGTCAAGTAG

Protein Analysis

338

Amino Acids

36.73

Weight (kDa)

7.62

Isoelectric Point (pI)

47.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_N PF00732 22 - 218 1.1e-13 GMC oxidoreductase
GMC_oxred_C PF05199 269 - 310 7.4e-07 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 174
Acc36I ACCTGC 1 cut(s) 935
AccB1I GGYRCC 1 cut(s) 545
AccB7I CCANNNNNTGG 2 cut(s) 281, 644
AciI CCGC 1 cut(s) 837
AclWI GGATC 8 cut(s) 231, 303, 421, 804, 817, 863, 926, 939
AcsI RAATTY 1 cut(s) 128
AfaI GTAC 1 cut(s) 513
AfiI CCNNNNNNNGG 4 cut(s) 220, 281, 644, 904
AflII CTTAAG 1 cut(s) 958
AflIII ACRYGT 1 cut(s) 159
AgsI TTSAA 2 cut(s) 892, 1002
AloI GAACNNNNNNTCC 2 cut(s) 479, 511
AluBI AGCT 6 cut(s) 26, 119, 359, 367, 461, 996
AluI AGCT 6 cut(s) 26, 119, 359, 367, 461, 996
Alw26I GTCTC 1 cut(s) 764
AlwI GGATC 8 cut(s) 231, 303, 421, 804, 817, 863, 926, 939
AlwNI CAGNNNCTG 1 cut(s) 69
AoxI GGCC 3 cut(s) 411, 586, 954
ApeKI GCWGC 2 cut(s) 367, 966
ApoI RAATTY 1 cut(s) 128
ArsI GACNNNNNNTTYG 2 cut(s) 479, 511
Asp700I GAANNNNTTC 1 cut(s) 51
AspS9I GGNCC 3 cut(s) 272, 495, 587
AsuC2I CCSGG 1 cut(s) 903
AsuHPI GGTGA 1 cut(s) 705
AsuII TTCGAA 1 cut(s) 485
AvaII GGWCC 2 cut(s) 272, 495
AxyI CCTNAGG 1 cut(s) 915
BamHI GGATCC 2 cut(s) 809, 931
BanI GGYRCC 1 cut(s) 545
BanII GRGCYC 1 cut(s) 560
BbvI GCAGC 2 cut(s) 354, 953
BccI CCATC 2 cut(s) 74, 642
BcgI CGANNNNNNTGC 2 cut(s) 435, 469
BclI TGATCA 1 cut(s) 93
BcnI CCSGG 1 cut(s) 903
BcoDI GTCTC 1 cut(s) 764
BfaI CTAG 2 cut(s) 704, 803
BfmI CTRYAG 1 cut(s) 240
BfrI CTTAAG 1 cut(s) 958
BfuAI ACCTGC 1 cut(s) 935
BglII AGATCT 1 cut(s) 382
BisI GCNGC 2 cut(s) 368, 967
BlsI GCNGC 2 cut(s) 369, 968
Bme1390I CCNGG 1 cut(s) 903
Bme18I GGWCC 2 cut(s) 272, 495
BmgT120I GGNCC 3 cut(s) 272, 495, 587
BmiI GGNNCC 5 cut(s) 497, 547, 557, 811, 933
BmrFI CCNGG 1 cut(s) 903
BmrI ACTGGG 1 cut(s) 614
BmsI GCATC 4 cut(s) 455, 868, 890, 953
BmuI ACTGGG 1 cut(s) 614
BplI GAGNNNNNCTC 2 cut(s) 746, 778
Bpu14I TTCGAA 1 cut(s) 485
BpuMI CCSGG 1 cut(s) 903
BsaJI CCNNGG 2 cut(s) 441, 902
Bsc4I CCNNNNNNNGG 4 cut(s) 220, 281, 644, 904
Bse118I RCCGGY 2 cut(s) 101, 542
Bse1I ACTGG 1 cut(s) 620
Bse21I CCTNAGG 1 cut(s) 915
Bse3DI GCAATG 1 cut(s) 863
BseDI CCNNGG 2 cut(s) 441, 902
BseGI GGATG 3 cut(s) 634, 881, 883
BseLI CCNNNNNNNGG 4 cut(s) 220, 281, 644, 904
BseMI GCAATG 1 cut(s) 863
BseMII CTCAG 1 cut(s) 929
BseNI ACTGG 1 cut(s) 620
BseXI GCAGC 2 cut(s) 354, 953
BseYI CCCAGC 1 cut(s) 69
BshFI GGCC 3 cut(s) 413, 588, 956
BshNI GGYRCC 1 cut(s) 545
BsiSI CCGG 3 cut(s) 102, 543, 902
BslFI GGGAC 3 cut(s) 170, 481, 615
BslI CCNNNNNNNGG 4 cut(s) 220, 281, 644, 904
BsmAI GTCTC 1 cut(s) 764
BsmFI GGGAC 3 cut(s) 170, 481, 615
BsnI GGCC 3 cut(s) 413, 588, 956
Bsp119I TTCGAA 1 cut(s) 485
Bsp1286I GDGCHC 1 cut(s) 560
Bsp143I GATC 9 cut(s) 93, 223, 295, 382, 426, 809, 855, 931, 976
BspACI CCGC 1 cut(s) 837
BspANI GGCC 3 cut(s) 413, 588, 956
BspCNI CTCAG 1 cut(s) 928
BspLI GGNNCC 5 cut(s) 497, 547, 557, 811, 933
BspMI ACCTGC 1 cut(s) 935
BspPI GGATC 8 cut(s) 231, 303, 421, 804, 817, 863, 926, 939
BspT104I TTCGAA 1 cut(s) 485
BspT107I GGYRCC 1 cut(s) 545
BspTI CTTAAG 1 cut(s) 958
BsrDI GCAATG 1 cut(s) 863
BsrFI RCCGGY 2 cut(s) 101, 542
BsrI ACTGG 1 cut(s) 620
BssAI RCCGGY 2 cut(s) 101, 542
BssECI CCNNGG 2 cut(s) 441, 902
BssMI GATC 9 cut(s) 93, 223, 295, 382, 426, 809, 855, 931, 976
BssT1I CCWWGG 1 cut(s) 441
Bst4CI ACNGT 1 cut(s) 418
Bst6I CTCTTC 2 cut(s) 437, 442
BstAFI CTTAAG 1 cut(s) 958
BstBI TTCGAA 1 cut(s) 485
BstC8I GCNNGC 2 cut(s) 103, 230
BstDEI CTNAG 3 cut(s) 76, 575, 915
BstF5I GGATG 3 cut(s) 634, 881, 883
BstKTI GATC 9 cut(s) 96, 226, 298, 385, 429, 812, 858, 934, 979
BstMAI GTCTC 1 cut(s) 764
BstMBI GATC 9 cut(s) 93, 223, 295, 382, 426, 809, 855, 931, 976
BstMWI GCNNNNNNNGC 1 cut(s) 410
BstNSI RCATGY 1 cut(s) 163
BstSCI CCNGG 1 cut(s) 901
BstSFI CTRYAG 1 cut(s) 240
BstV1I GCAGC 2 cut(s) 354, 953
BstX2I RGATCY 4 cut(s) 382, 426, 809, 931
BstYI RGATCY 4 cut(s) 382, 426, 809, 931
Bsu36I CCTNAGG 1 cut(s) 915
BsuRI GGCC 3 cut(s) 413, 588, 956
BtsCI GGATG 3 cut(s) 634, 881, 883
BveI ACCTGC 1 cut(s) 935
Cac8I GCNNGC 2 cut(s) 103, 230
CaiI CAGNNNCTG 1 cut(s) 69
Cfr10I RCCGGY 2 cut(s) 101, 542
Cfr13I GGNCC 3 cut(s) 272, 495, 587
Csp6I GTAC 1 cut(s) 512
CviAII CATG 4 cut(s) 91, 160, 353, 472
CviQI GTAC 1 cut(s) 512
DdeI CTNAG 3 cut(s) 76, 575, 915
DpnI GATC 9 cut(s) 95, 225, 297, 384, 428, 811, 857, 933, 978
DpnII GATC 9 cut(s) 93, 223, 295, 382, 426, 809, 855, 931, 976
Eam1104I CTCTTC 2 cut(s) 437, 442
EarI CTCTTC 2 cut(s) 437, 442
Eco130I CCWWGG 1 cut(s) 441
Eco24I GRGCYC 1 cut(s) 560
Eco47I GGWCC 2 cut(s) 272, 495
Eco81I CCTNAGG 1 cut(s) 915
EcoO109I RGGNCCY 1 cut(s) 495
EcoRI GAATTC 1 cut(s) 128
EcoT14I CCWWGG 1 cut(s) 441
EcoT22I ATGCAT 2 cut(s) 865, 883
EcoT38I GRGCYC 1 cut(s) 560
ErhI CCWWGG 1 cut(s) 441
FaeI CATG 4 cut(s) 94, 163, 356, 475
FalI AAGNNNNNCTT 4 cut(s) 116, 148, 416, 448
FaqI GGGAC 3 cut(s) 170, 481, 615
FatI CATG 4 cut(s) 90, 159, 352, 471
FauNDI CATATG 1 cut(s) 508
FbaI TGATCA 1 cut(s) 93
Fnu4HI GCNGC 2 cut(s) 368, 967
FokI GGATG 3 cut(s) 621, 868, 890
FriOI GRGCYC 1 cut(s) 560
Fsp4HI GCNGC 2 cut(s) 368, 967
FspBI CTAG 2 cut(s) 704, 803
GluI GCNGC 2 cut(s) 368, 967
GsaI CCCAGC 1 cut(s) 73
HaeIII GGCC 3 cut(s) 413, 588, 956
HapII CCGG 3 cut(s) 102, 543, 902
Hin1II CATG 4 cut(s) 94, 163, 356, 475
HinfI GANTC 5 cut(s) 468, 482, 593, 757, 910
HpaII CCGG 3 cut(s) 102, 543, 902
HphI GGTGA 1 cut(s) 705
Hpy166II GTNNAC 1 cut(s) 36
Hpy188I TCNGA 3 cut(s) 43, 65, 387
Hpy188III TCNNGA 2 cut(s) 275, 917
Hpy8I GTNNAC 1 cut(s) 36
Hpy99I CGWCG 1 cut(s) 331
HpyAV CCTTC 2 cut(s) 41, 720
HpyCH4III ACNGT 1 cut(s) 418
HpyCH4IV ACGT 1 cut(s) 329
HpyCH4V TGCA 5 cut(s) 404, 506, 863, 881, 969
HpyF10VI GCNNNNNNNGC 1 cut(s) 410
HpyF3I CTNAG 3 cut(s) 76, 575, 915
HpySE526I ACGT 1 cut(s) 329
Hsp92II CATG 4 cut(s) 94, 163, 356, 475
KroI GCCGGC 1 cut(s) 101
KroNI GCCGGC 1 cut(s) 103
Ksp22I TGATCA 1 cut(s) 93
Kzo9I GATC 9 cut(s) 93, 223, 295, 382, 426, 809, 855, 931, 976
LmnI GCTCC 3 cut(s) 356, 364, 555
Lsp1109I GCAGC 2 cut(s) 354, 953
LweI GCATC 4 cut(s) 455, 868, 890, 953
MaeI CTAG 2 cut(s) 704, 803
MaeII ACGT 1 cut(s) 329
MaeIII GTNAC 1 cut(s) 671
MalI GATC 9 cut(s) 95, 225, 297, 384, 428, 811, 857, 933, 978
MboI GATC 9 cut(s) 93, 223, 295, 382, 426, 809, 855, 931, 976
MboII GAAGA 7 cut(s) 17, 56, 206, 424, 429, 436, 733
MflI RGATCY 4 cut(s) 382, 426, 809, 931
MhlI GDGCHC 1 cut(s) 560
MluCI AATT 6 cut(s) 128, 289, 315, 392, 678, 892
MlyI GAGTC 1 cut(s) 766
MmeI TCCRAC 1 cut(s) 799
MnlI CCTC 7 cut(s) 59, 253, 440, 486, 520, 570, 924
Mph1103I ATGCAT 2 cut(s) 865, 883
MroNI GCCGGC 1 cut(s) 101
MroXI GAANNNNTTC 1 cut(s) 51
MseI TTAA 7 cut(s) 288, 303, 372, 612, 677, 681, 959
MslI CAYNNNNRTG 2 cut(s) 184, 926
MspA1I CMGCKG 1 cut(s) 119
MspCI CTTAAG 1 cut(s) 958
MspI CCGG 3 cut(s) 102, 543, 902
MspR9I CCNGG 1 cut(s) 903
MwoI GCNNNNNNNGC 1 cut(s) 410
NaeI GCCGGC 1 cut(s) 103
NciI CCSGG 1 cut(s) 903
NdeI CATATG 1 cut(s) 508
NdeII GATC 9 cut(s) 93, 223, 295, 382, 426, 809, 855, 931, 976
NgoMIV GCCGGC 1 cut(s) 101
NlaIII CATG 4 cut(s) 94, 163, 356, 475
NlaIV GGNNCC 5 cut(s) 497, 547, 557, 811, 933
NsiI ATGCAT 2 cut(s) 865, 883
NspI RCATGY 1 cut(s) 163
NspV TTCGAA 1 cut(s) 485
PacI TTAATTAA 1 cut(s) 681
PciI ACATGT 1 cut(s) 159
PdiI GCCGGC 1 cut(s) 103
PdmI GAANNNNTTC 1 cut(s) 51
PfeI GAWTC 4 cut(s) 468, 482, 593, 910
PflMI CCANNNNNTGG 2 cut(s) 281, 644
PkrI GCNGC 2 cut(s) 369, 968
PleI GAGTC 1 cut(s) 765
PpsI GAGTC 1 cut(s) 765
PpuMI RGGWCCY 1 cut(s) 495
PscI ACATGT 1 cut(s) 159
PsiI TTATAA 1 cut(s) 174
Psp5II RGGWCCY 1 cut(s) 495
PspFI CCCAGC 1 cut(s) 69
PspN4I GGNNCC 5 cut(s) 497, 547, 557, 811, 933
PspPI GGNCC 3 cut(s) 272, 495, 587
PspPPI RGGWCCY 1 cut(s) 495
PstNI CAGNNNCTG 1 cut(s) 69
PsuI RGATCY 4 cut(s) 382, 426, 809, 931
PvuII CAGCTG 1 cut(s) 119
RsaI GTAC 1 cut(s) 513
RsaNI GTAC 1 cut(s) 512
RseI CAYNNNNRTG 2 cut(s) 184, 926
SaqAI TTAA 7 cut(s) 288, 303, 372, 612, 677, 681, 959
SatI GCNGC 2 cut(s) 368, 967
Sau3AI GATC 9 cut(s) 93, 223, 295, 382, 426, 809, 855, 931, 976
Sau96I GGNCC 3 cut(s) 272, 495, 587
SchI GAGTC 1 cut(s) 766
ScrFI CCNGG 1 cut(s) 903
SduI GDGCHC 1 cut(s) 560
SfaNI GCATC 4 cut(s) 455, 868, 890, 953
SfcI CTRYAG 1 cut(s) 240
SfuI TTCGAA 1 cut(s) 485
SinI GGWCC 2 cut(s) 272, 495
SmiMI CAYNNNNRTG 2 cut(s) 184, 926
SmlI CTYRAG 1 cut(s) 958
SmoI CTYRAG 1 cut(s) 958
Sse9I AATT 6 cut(s) 128, 289, 315, 392, 678, 892
SsiI CCGC 1 cut(s) 837
SspMI CTAG 2 cut(s) 704, 803
StyD4I CCNGG 1 cut(s) 901
StyI CCWWGG 1 cut(s) 441
TaaI ACNGT 1 cut(s) 418
TaiI ACGT 1 cut(s) 332
TaqI TCGA 2 cut(s) 326, 485
TasI AATT 6 cut(s) 128, 289, 315, 392, 678, 892
TfiI GAWTC 4 cut(s) 468, 482, 593, 910
Tru1I TTAA 7 cut(s) 288, 303, 372, 612, 677, 681, 959
Tru9I TTAA 7 cut(s) 288, 303, 372, 612, 677, 681, 959
TseI GCWGC 2 cut(s) 367, 966
TspDTI ATGAA 1 cut(s) 152
Van91I CCANNNNNTGG 2 cut(s) 281, 644
Vha464I CTTAAG 1 cut(s) 958
VpaK11BI GGWCC 2 cut(s) 272, 495
XapI RAATTY 1 cut(s) 128
XceI RCATGY 1 cut(s) 163
XcmI CCANNNNNNNNNTGG 1 cut(s) 641
XmnI GAANNNNTTC 1 cut(s) 51
XspI CTAG 2 cut(s) 704, 803
Zsp2I ATGCAT 2 cut(s) 865, 883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.