Rorug04G0045600

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000533
Physical Location & Seq
Reverse (-)
7086519 .. 7094150
7632 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0045600.1

Sequence Viewer

Length: 372 bp
ATGAACAGTGATACAACTCTTTTTTTCCTTTCCGGATTTGCCCTCAAAACAGATAAACATGGGGTTTTATACTGTTCTGCTGTGACGCTTTTGTCTCTTCTGCCGCTTTGGTCGGTTTCGTCAGAGAAGAGAAAGAAGAAGGAGGAGGAGGAGGAGACGGGAAGCTCTCTTTCCTTTCAGAGGTCCGCTCACTGCTCGGGTTTTAATTGGAAAAATGGTGGTGTAGACTCTGCATGCAGTCCCAGCGCTGCCTCACCTTCCCCGTCATACTCCGGCGAGCCATTAAAACGTACTCAGGAGTCAGATCAAGAGGAGCCTCACATGAAAAGGAAGGTGCTTGCTGAGGCCAAATTGAGGATGGGGATTGACTGA

Protein Analysis

123

Amino Acids

13.52

Weight (kDa)

6.83

Isoelectric Point (pI)

61.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 91
AccBSI CCGCTC 1 cut(s) 188
AccI GTMKAC 1 cut(s) 225
AccIII TCCGGA 1 cut(s) 32
AciI CCGC 2 cut(s) 104, 186
AfaI GTAC 1 cut(s) 292
AfeI AGCGCT 1 cut(s) 247
AfiI CCNNNNNNNGG 2 cut(s) 180, 354
AluBI AGCT 1 cut(s) 165
AluI AGCT 1 cut(s) 165
Alw26I GTCTC 2 cut(s) 99, 149
Ama87I CYCGRG 1 cut(s) 196
Aor13HI TCCGGA 1 cut(s) 32
Aor51HI AGCGCT 1 cut(s) 247
AoxI GGCC 1 cut(s) 345
ApeKI GCWGC 1 cut(s) 248
AspLEI GCGC 1 cut(s) 248
AspS9I GGNCC 1 cut(s) 183
AsuHPI GGTGA 1 cut(s) 246
AvaI CYCGRG 1 cut(s) 196
AvaII GGWCC 1 cut(s) 183
BbvCI CCTCAGC 1 cut(s) 342
BbvI GCAGC 1 cut(s) 235
BccI CCATC 1 cut(s) 352
BcoDI GTCTC 2 cut(s) 99, 149
BfoI RGCGCY 1 cut(s) 249
BisI GCNGC 2 cut(s) 104, 249
BlsI GCNGC 2 cut(s) 105, 250
Bme18I GGWCC 1 cut(s) 183
BmeT110I CYCGRG 1 cut(s) 196
BmgT120I GGNCC 1 cut(s) 183
BmiI GGNNCC 1 cut(s) 315
BplI GAGNNNNNCTC 2 cut(s) 172, 204
Bpu10I CCTNAGC 1 cut(s) 342
BsaWI WCCGGW 1 cut(s) 32
Bsc4I CCNNNNNNNGG 2 cut(s) 180, 354
BseAI TCCGGA 1 cut(s) 32
BseGI GGATG 1 cut(s) 363
BseLI CCNNNNNNNGG 2 cut(s) 180, 354
BseMII CTCAG 2 cut(s) 308, 333
BseRI GAGGAG 5 cut(s) 158, 161, 164, 167, 326
BseXI GCAGC 1 cut(s) 235
BseYI CCCAGC 1 cut(s) 242
BshFI GGCC 1 cut(s) 347
BsiHKCI CYCGRG 1 cut(s) 196
BsiSI CCGG 2 cut(s) 33, 273
BslFI GGGAC 1 cut(s) 225
BslI CCNNNNNNNGG 2 cut(s) 180, 354
BsmAI GTCTC 2 cut(s) 99, 149
BsmBI CGTCTC 1 cut(s) 149
BsmFI GGGAC 1 cut(s) 225
BsnI GGCC 1 cut(s) 347
BsoBI CYCGRG 1 cut(s) 196
Bsp13I TCCGGA 1 cut(s) 32
Bsp143I GATC 1 cut(s) 304
BspACI CCGC 2 cut(s) 104, 186
BspANI GGCC 1 cut(s) 347
BspCNI CTCAG 2 cut(s) 307, 334
BspEI TCCGGA 1 cut(s) 32
BspLI GGNNCC 1 cut(s) 315
BsrBI CCGCTC 1 cut(s) 188
BssMI GATC 1 cut(s) 304
Bst4CI ACNGT 2 cut(s) 8, 74
Bst6I CTCTTC 2 cut(s) 102, 122
BstC8I GCNNGC 3 cut(s) 235, 278, 339
BstDEI CTNAG 2 cut(s) 294, 342
BstENI CCTNNNNNAGG 1 cut(s) 178
BstF5I GGATG 1 cut(s) 363
BstH2I RGCGCY 1 cut(s) 249
BstHHI GCGC 1 cut(s) 248
BstKTI GATC 1 cut(s) 307
BstMAI GTCTC 2 cut(s) 99, 149
BstMBI GATC 1 cut(s) 304
BstMWI GCNNNNNNNGC 1 cut(s) 243
BstNSI RCATGY 1 cut(s) 237
BstV1I GCAGC 1 cut(s) 235
BsuRI GGCC 1 cut(s) 347
BtsCI GGATG 1 cut(s) 363
BtsI GCAGTG 1 cut(s) 190
BtsIMutI CAGTG 2 cut(s) 13, 190
Cac8I GCNNGC 3 cut(s) 235, 278, 339
CfoI GCGC 1 cut(s) 248
Cfr13I GGNCC 1 cut(s) 183
CseI GACGC 1 cut(s) 94
Csp6I GTAC 1 cut(s) 291
CviAII CATG 3 cut(s) 59, 234, 322
CviJI RGCY 4 cut(s) 165, 280, 316, 347
CviKI_1 RGCY 4 cut(s) 165, 280, 316, 347
CviQI GTAC 1 cut(s) 291
DdeI CTNAG 2 cut(s) 294, 342
DpnI GATC 1 cut(s) 306
DpnII GATC 1 cut(s) 304
DrdI GACNNNNNNGTC 1 cut(s) 91
DseDI GACNNNNNNGTC 1 cut(s) 91
Eam1104I CTCTTC 2 cut(s) 102, 122
EarI CTCTTC 2 cut(s) 102, 122
Eco47I GGWCC 1 cut(s) 183
Eco47III AGCGCT 1 cut(s) 247
Eco88I CYCGRG 1 cut(s) 196
EcoNI CCTNNNNNAGG 1 cut(s) 178
Esp3I CGTCTC 1 cut(s) 149
FaeI CATG 3 cut(s) 62, 237, 325
FaiI YATR 5 cut(s) 60, 70, 235, 268, 323
FaqI GGGAC 1 cut(s) 225
FatI CATG 3 cut(s) 58, 233, 321
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 2 cut(s) 104, 249
Fsp4HI GCNGC 2 cut(s) 104, 249
GlaI GCGC 1 cut(s) 247
GluI GCNGC 2 cut(s) 104, 249
GsaI CCCAGC 1 cut(s) 246
HaeII RGCGCY 1 cut(s) 249
HaeIII GGCC 1 cut(s) 347
HapII CCGG 2 cut(s) 33, 273
HgaI GACGC 1 cut(s) 94
HhaI GCGC 1 cut(s) 248
Hin1II CATG 3 cut(s) 62, 237, 325
Hin6I GCGC 1 cut(s) 246
HinP1I GCGC 1 cut(s) 246
HinfI GANTC 2 cut(s) 227, 299
HpaII CCGG 2 cut(s) 33, 273
HphI GGTGA 1 cut(s) 246
Hpy166II GTNNAC 1 cut(s) 226
Hpy188I TCNGA 3 cut(s) 124, 180, 304
Hpy188III TCNNGA 3 cut(s) 33, 296, 308
Hpy8I GTNNAC 1 cut(s) 226
HpyAV CCTTC 3 cut(s) 133, 267, 325
HpyCH4III ACNGT 2 cut(s) 8, 74
HpyCH4IV ACGT 1 cut(s) 289
HpyCH4V TGCA 2 cut(s) 233, 237
HpyF10VI GCNNNNNNNGC 1 cut(s) 243
HpyF3I CTNAG 2 cut(s) 294, 342
HpySE526I ACGT 1 cut(s) 289
Hsp92II CATG 3 cut(s) 62, 237, 325
HspAI GCGC 1 cut(s) 246
Kpn2I TCCGGA 1 cut(s) 32
Kzo9I GATC 1 cut(s) 304
LmnI GCTCC 1 cut(s) 313
LpnPI CCDG 4 cut(s) 46, 256, 281, 286
Lsp1109I GCAGC 1 cut(s) 235
MaeII ACGT 1 cut(s) 289
MaeIII GTNAC 1 cut(s) 82
MalI GATC 1 cut(s) 306
MbiI CCGCTC 1 cut(s) 188
MboI GATC 1 cut(s) 304
MboII GAAGA 3 cut(s) 89, 139, 148
MluCI AATT 2 cut(s) 205, 350
MlyI GAGTC 2 cut(s) 221, 308
MroI TCCGGA 1 cut(s) 32
MseI TTAA 2 cut(s) 204, 284
MspI CCGG 2 cut(s) 33, 273
MwoI GCNNNNNNNGC 1 cut(s) 243
NdeII GATC 1 cut(s) 304
NlaIII CATG 3 cut(s) 62, 237, 325
NlaIV GGNNCC 1 cut(s) 315
NmuCI GTSAC 1 cut(s) 82
NspI RCATGY 1 cut(s) 237
PaeI GCATGC 1 cut(s) 237
PkrI GCNGC 2 cut(s) 105, 250
PleI GAGTC 2 cut(s) 221, 307
PpsI GAGTC 2 cut(s) 221, 307
PspFI CCCAGC 1 cut(s) 242
PspN4I GGNNCC 1 cut(s) 315
PspPI GGNCC 1 cut(s) 183
RsaI GTAC 1 cut(s) 292
RsaNI GTAC 1 cut(s) 291
SaqAI TTAA 2 cut(s) 204, 284
SatI GCNGC 2 cut(s) 104, 249
Sau3AI GATC 1 cut(s) 304
Sau96I GGNCC 1 cut(s) 183
SchI GAGTC 2 cut(s) 221, 308
SetI ASST 5 cut(s) 167, 185, 259, 292, 336
SinI GGWCC 1 cut(s) 183
SphI GCATGC 1 cut(s) 237
Sse9I AATT 2 cut(s) 205, 350
SsiI CCGC 2 cut(s) 104, 186
TaaI ACNGT 2 cut(s) 8, 74
TaiI ACGT 1 cut(s) 292
TasI AATT 2 cut(s) 205, 350
TauI GCSGC 1 cut(s) 106
Tru1I TTAA 2 cut(s) 204, 284
Tru9I TTAA 2 cut(s) 204, 284
TscAI CASTG 2 cut(s) 13, 197
TseFI GTSAC 1 cut(s) 82
TseI GCWGC 1 cut(s) 248
Tsp45I GTSAC 1 cut(s) 82
TspDTI ATGAA 2 cut(s) 17, 338
TspRI CASTG 2 cut(s) 13, 197
VpaK11BI GGWCC 1 cut(s) 183
XagI CCTNNNNNAGG 1 cut(s) 178
XceI RCATGY 1 cut(s) 237
XcmI CCANNNNNNNNNTGG 1 cut(s) 355
XmiI GTMKAC 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.